CN103650895A - Cutting breeding method of pseudostellaria heterophylla - Google Patents
Cutting breeding method of pseudostellaria heterophylla Download PDFInfo
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- CN103650895A CN103650895A CN201310742279.6A CN201310742279A CN103650895A CN 103650895 A CN103650895 A CN 103650895A CN 201310742279 A CN201310742279 A CN 201310742279A CN 103650895 A CN103650895 A CN 103650895A
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Abstract
The invention relates to a cutting breeding method of pseudostellaria heterophylla. The cutting breeding method of pseudostellaria heterophylla comprises the following steps that in a production greenhouse of virus-free pseudostellaria heterophylla protospecies, strong seedlings with the height being 12-20cm and the ground stem being larger than 1mm and with more than six joints are selected, and a stem section which grows for 4-6cm and has more than four joints is cut to serve as a cutting twig; a seedling bed is prepared; the matrix of the seedling bed is humus soil and perlite, and the ratio between the humus soil and the perlite is 3:1; an cutting operation is carried out, cutting is carried out in the direction perpendicular to the moving direction of a box surface on the seedling bed with the disinfected matrix; a small shed is built after cutting is finished, the humidity is controlled to be about 90%, the temperature is controlled to be 20+/-2 DEG C, the small shed is dismounted after one week, an insect-prevention facility is built, and then common field management on water, fertilizer and the like is carried out. The cutting breeding method of pseudostellaria heterophylla is convenient to operate, high in survival rate, short in period, and suitable for facility planting.
Description
Technical field
The present invention relates to planting technology field, relate in particular to a kind of cuttage breeding method of radix pseudostellariae.
Background technology
Radix pseudostellariae is pinkwort caryophyllaceous ginseng
pseudostellaria heterophylla(Miq.) the piece root of Pax ex Pax et Hoffm..When summer, cauline leaf was most of withered, excavate piece root and clean, remove fibrous root, put after slightly scalding in boiling water and dry or directly dry rear hyoscine.Radix pseudostellariae is gone through an edition < < Pharmacopoeia of People's Republic of China > > and is all given and recording.Radix pseudostellariae has and replenishes qi to invigorate the spleen, promoting production of body fluid and nourishing the lung function; Clinical tired for insufficiency of the spleen body, poor appetite, weak after being ill, deficiency of both vital energy and Yin, the disease such as spontaneous perspiration day is thirsty, dryness of the lung dry cough.Radix pseudostellariae consumption cumulative year after year, market prospects are wide.
The mating system of radix pseudostellariae mainly contains sexual reproduction (seminal propagation) and vegetative propagation (piece root propagation) at present.
Since the wild change of radix pseudostellariae man plants, Production of Large Fields mainly adopts piece root to carry out vegetative propagation, and this has caused radix pseudostellariae kind constantly to be degenerated, and sick worm harm constantly aggravates and then has a strong impact on radix pseudostellariae per unit area yield and quality.In addition, seminal propagation is also one of mode of producing offspring of radix pseudostellariae, because its Capsule maturity is inconsistent, Chang Ziran is scattered, if artificially collect seed by time and effort consuming, still lack at present the treatment technology of radix pseudostellariae seed being abolished to dormancy, this cause the germination rate of radix pseudostellariae seed and germination vigor all lower, then affect output, at present only after commodity ginsengs is gathered or during asexual cultivation, with regard to the seed naturally landing, adopt the method for covering railway carriage or compartment to plant, as auxiliary cultivation step.Therefore, find a kind ofly make the quality of radix pseudostellariae high, propagation method stable yield, breeding on a large scale is current urgent problem.
Summary of the invention
The object of the invention is to overcome above-mentioned shortcoming and provide a kind of easy to operate, survival rate is high, the cycle is short, is applicable to the radix pseudostellariae cuttage breeding method of facility cultivation.
The cuttage breeding method of a kind of radix pseudostellariae of the present invention, comprises the following steps:
(1) in detoxification radix pseudostellariae seed production booth, the high 12 ~ 20cm of roguing, ground stem is greater than 1 ㎜, 6 healthy and strong plant more than joint, with scissors cut into be about 4 ~ 6cm with 4 stem section more than joint, be positioned in the container of sterilization.Seedling by under shearing, is trimmed to lower end otch and pitch 0.3cm, cuts off the blade of 1～2 joint in lower end simultaneously, and cuttage bar is classified according to clip position, is bundled into wisp, lower end otch alignment, smooth; Cuttage bar is soaked to cuttage bar end 1 minute with the carbendazim (75% wetting powder) of 1000 times of liquid, be filtered dry and be positioned over clean containers for future use;
(2) preparation in seedbed: medium of seedling bed is humus soil and perlite, ratio is 3:1, first by matrix scarifying, every square metre must speed goes out after fine granule 10-15g adds water 3L dilution and sprays with 98%-100%, then the matrix of stirring 7-10cm, medicament is fully mixed with matrix, and covered with plastic film, got final product cuttage after scarifying matrix 2 times after one week;
(3) carry out cuttage operation, Shang Yan railway carriage or compartment, the seedbed face of having sterilized in matrix moves towards vertical direction and carries out cuttage, and cottage method is:
Rod with diameter 0.3 ~ 0.5cm on seedbed bores dark 3 ~ 4cm aperture, in methyl α-naphthyl acetate (NAA) solution that is 1000ppm in concentration by the cuttage bar of handling well, dip in and put into afterwards aperture, limit insert edge compacting, control spacing in the rows 6 ~ 8cm, line-spacing 8 ~ 10cm, irrigates with 1/10MS minimal medium solution after cuttage;
Or on seedbed, open the shallow ridges of dark 3 ~ 4cm, in the NAA solution that is 1000ppm in concentration by the cuttage bar of handling well, dip in and by spacing in the rows 6 ~ 8cm, be placed in shallow ridges afterwards, earthing compacting, then opens shallow ridges by line-spacing 8 ~ 10cm, carry out successively, after cuttage, with 1/10MS minimal medium solution, irrigate;
(4) after cuttage, build little shed, humidity control is in 90% left and right, and temperature is controlled at 20 ± 2 ℃ of left and right, removes little shed after one week, sets up insect protected facility, then carries out the field management such as daily water, fertilizer.
In order to verify the exercising result of said method to the cultivation of radix pseudostellariae cottage propagation, carried out series of experimental research, specific as follows:
Separate sources cuttage bar has been infected by the virus and has contacted directly radix pseudostellariae piece root, it is research object that this test adopts conventional piece root propagation branch, group training detoxic seedling branch, radix pseudostellariae seed seedling branch, according to identical cutting density, every group of cuttage 3 ㎡ are a processing, repeat for 3 times, cuttage is front by slotting machine matrix sterilization, after cuttage, set up insect protected facility, and added up survival rate after 20 days, in mid-May, observe blade performance, late July surveys and to produce and to detect the radix pseudostellariae piece root situation that is infected by the virus, and statistical conditions are as following table:
By above data, can be found out, cuttage survival rate aspect, detoxification radix pseudostellariae is all high compared with other two kinds compared with branch, reaches 98%, and other branches in survival rate in 90% left and right; Conventional piece root propagation branch, radix pseudostellariae seed seedling branch plant growth blade in mid-term all part shows floral leaf, mottled floral leaf, shrinkage, distortion distorts and have on the phenomenon of curling trend, this is the result being infected by the virus, and by determination block root Virus Infection, has obtained further confirmation; Owing to endangered by virus disease, the growth of plant has been subject to having a strong impact on, and then affects the growth of underground root, and the output of detoxification radix pseudostellariae group training seedling branch is the highest, reaches 689.78g/ ㎡, and the output of radix pseudostellariae seed seedling branch is minimum, at 498.25g/ ㎡.
The present invention compared with prior art, has obvious beneficial effect, as can be known from the above technical solutions: the present invention is easy to operate, cutting survival, survival rate is high, and reproduction coefficient reaches 6 ~ 10 times, and speed is fast, cutting propagation is applicable to radix pseudostellariae facility cultivation, can output produce with seed ginseng and medicinal material commodity then.The heavy 0.45g of the average simple grain of radix pseudostellariae piece root that uses the present invention to produce.Can be for radix pseudostellariae Production of Large Fields provides good seed ginseng, the seed ginseng that uses the present invention to breed, land for growing field crops growth coefficient reaches 10 ~ 15 times.
Embodiment
embodiment 1:
A cuttage breeding method for radix pseudostellariae, comprises the following steps:
(1) in detoxification radix pseudostellariae seed production booth, the high 12 ~ 20cm of roguing, ground stem is greater than 1 ㎜, 6 healthy and strong plant more than joint, with scissors cut into be about 4 ~ 6cm with 4 stem section more than joint, be positioned in the container of sterilization.Seedling by under shearing, is trimmed to lower end otch and pitch 0.3cm, cuts off the blade of 1～2 joint in lower end simultaneously, and cuttage bar is classified according to clip position, is bundled into wisp, lower end otch alignment, smooth; Cuttage bar is soaked to cuttage bar end 1 minute with the carbendazim (75% wetting powder) of 1000 times of liquid, be filtered dry and be positioned over clean containers for future use;
(2) preparation in seedbed: medium of seedling bed is humus soil and perlite, ratio is 3:1, first by matrix scarifying, every square metre must speed goes out after fine granule 10-15g adds water 3L dilution and sprays with 98%-100%, and the matrix of then stirring 7-10cm, makes medicament fully mix with matrix, covered with plastic film, got final product cuttage after scarifying matrix 2 times after 1 week;
(3) carry out cuttage operation, Shang Yan railway carriage or compartment, the seedbed face of having sterilized in matrix moves towards vertical direction and carries out cuttage, and cottage method is:
Rod with diameter 0.3 ~ 0.5cm on seedbed bores dark 3 ~ 4cm aperture, in methyl α-naphthyl acetate (NAA) solution that is 1000ppm in concentration by the cuttage bar of handling well, dip in and put into afterwards aperture, limit insert edge compacting, control spacing in the rows 6 ~ 8cm, line-spacing 8 ~ 10cm, irrigates with 1/10MS minimal medium solution after cuttage;
(4) after cuttage, build little shed, humidity control is in 90% left and right, and temperature is controlled at 20 ± 2 ℃ of left and right, removes little shed after one week, sets up insect protected facility, then carries out the field management such as daily water, fertilizer.
embodiment 2:
A cuttage breeding method for radix pseudostellariae, comprises the following steps:
(1) in detoxification radix pseudostellariae seed production booth, the high 12 ~ 20cm of roguing, ground stem is greater than 1 ㎜, 6 healthy and strong plant more than joint, with scissors cut into be about 4 ~ 6cm with 4 stem section more than joint, be positioned in the container of sterilization.Seedling by under shearing, is trimmed to lower end otch and pitch 0.3cm, cuts off the blade of 1～2 joint in lower end simultaneously, and cuttage bar is classified according to clip position, is bundled into wisp, lower end otch alignment, smooth; Cuttage bar is soaked to cuttage bar end 1 minute with the carbendazim (75% wetting powder) of 1000 times of liquid, be filtered dry and be positioned over clean containers for future use;
(2) preparation in seedbed: medium of seedling bed is humus soil and perlite, ratio is 3:1, first by matrix scarifying, every square metre must speed goes out after fine granule 10-15g adds water 3L dilution and sprays with 98%-100%, and the matrix of then stirring 7-10cm, makes medicament fully mix with matrix, covered with plastic film, got final product cuttage after scarifying matrix 2 times after 1 week;
(3) carry out cuttage operation, Shang Yan railway carriage or compartment, the seedbed face of having sterilized in matrix moves towards vertical direction and carries out cuttage, cottage method is: the shallow ridges of opening dark 3 ~ 4cm on seedbed, in methyl α-naphthyl acetate (NAA) solution that is 1000ppm in concentration by the cuttage bar of handling well, dip in and by spacing in the rows 6 ~ 8cm, be placed in shallow ridges afterwards, earthing compacting, then by line-spacing 8 ~ 10cm, open shallow ridges, carry out successively, after cuttage, with 1/10MS minimal medium solution, irrigate;
(4) after cuttage, build little shed, humidity control is in 90% left and right, and temperature is controlled at 20 ± 2 ℃ of left and right, removes little shed after one week, sets up insect protected facility, then carries out the field management such as daily water, fertilizer.
Claims (2)
1. a cuttage breeding method for radix pseudostellariae, comprises the following steps:
(1) in detoxification radix pseudostellariae seed production booth, high 12 ~ the 20cm of roguing, ground stem is greater than 1 ㎜, 6 healthy and strong plant more than joint, cut the 4 ~ 6cm that grows up with 4 stem section more than joint, be positioned in the container of sterilization, the seedling by under shearing, is trimmed to lower end otch and pitch 0.3cm, cut off the blade of 1～2 joint in lower end simultaneously, cuttage bar is classified according to clip position, be bundled into wisp, lower end otch alignment, smooth; Cuttage bar is soaked to cuttage bar end 1 minute with carbendazim 75% wetting powder of 1000 times of liquid, be filtered dry and be positioned over clean containers for future use;
(2) preparation in seedbed: medium of seedling bed is humus soil and perlite, ratio is 3:1, first by matrix scarifying, every square metre must speed goes out after fine granule 10-15g adds water 3L dilution and sprays with 98%-100%, then the matrix of stirring 7-10cm, medicament is fully mixed with matrix, and covered with plastic film, got final product cuttage after scarifying matrix 2 times after 1 week;
(3) carry out cuttage operation, Shang Yan railway carriage or compartment, the seedbed face of having sterilized in matrix moves towards vertical direction and carries out cuttage, cottage method is: the rod with diameter 0.3 ~ 0.5cm on seedbed bores dark 3 ~ 4cm aperture, in the naphthalene acid solution that is 1000ppm in concentration by the cuttage bar of handling well, dip in and put into afterwards aperture, limit insert edge compacting, control spacing in the rows 6 ~ 8cm, line-spacing 8 ~ 10cm, irrigates with 1/10MS minimal medium solution after cuttage;
(4) after cuttage, build little shed, humidity control is in 90% left and right, and temperature is controlled at 20 ± 2 ℃, removes little shed after one week, sets up insect protected facility, then carries out daily water, fertile field management.
2. the cuttage breeding method of a kind of radix pseudostellariae as claimed in claim 1, wherein cottage method is: the shallow ridges of opening dark 3 ~ 4cm on seedbed, in the naphthalene acid solution that is 1000ppm in concentration by the cuttage bar of handling well, dip in and by spacing in the rows 6 ~ 8cm, be placed in shallow ridges afterwards, earthing compacting, then by line-spacing 8 ~ 10cm, open shallow ridges, carry out successively, after cuttage, with 1/10MS minimal medium solution, irrigate.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104380965A (en) * | 2014-11-03 | 2015-03-04 | 贵州省植物园 | Production method for radix pseudostellariae roots |
| CN105123199A (en) * | 2015-08-23 | 2015-12-09 | 林平 | Cutting seedling growing method for stems below top shoots of stevia rebaudiana |
| CN106258295A (en) * | 2015-06-24 | 2017-01-04 | 黄保成 | A kind of method for culturing seedlings of Brachystemma calycina D. Don. |
| CN108377772A (en) * | 2018-02-09 | 2018-08-10 | 贵州兴康农业科技有限公司 | A kind of cuttage breeding method of Chinese mesona herb |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104380965A (en) * | 2014-11-03 | 2015-03-04 | 贵州省植物园 | Production method for radix pseudostellariae roots |
| CN106258295A (en) * | 2015-06-24 | 2017-01-04 | 黄保成 | A kind of method for culturing seedlings of Brachystemma calycina D. Don. |
| CN105123199A (en) * | 2015-08-23 | 2015-12-09 | 林平 | Cutting seedling growing method for stems below top shoots of stevia rebaudiana |
| CN108377772A (en) * | 2018-02-09 | 2018-08-10 | 贵州兴康农业科技有限公司 | A kind of cuttage breeding method of Chinese mesona herb |
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Effective date of registration: 20160810 Address after: 557500, Guizhou Town, Danzhai County, Xingren province camp village Patentee after: Chang Huang Huang (Guizhou) Chinese Medicine Co., Ltd. Address before: 556013 box 204, Qiandongnan Miao and Dong Autonomous Prefecture, Guizhou, Kaili Patentee before: Guizhou Changhao Traditional Chinses Medicine Development Co.,Ltd. |
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