CN103642658B - A kind of establishing-Yang Composite fermentation type Epimedium health wine and production technique thereof - Google Patents

A kind of establishing-Yang Composite fermentation type Epimedium health wine and production technique thereof Download PDF

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CN103642658B
CN103642658B CN201310604612.7A CN201310604612A CN103642658B CN 103642658 B CN103642658 B CN 103642658B CN 201310604612 A CN201310604612 A CN 201310604612A CN 103642658 B CN103642658 B CN 103642658B
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health wine
epimedium
bark
liquid
saccharification
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CN103642658A (en
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董书阁
段智岗
王金波
侯文杰
王志伟
李佳
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Li Meihuan
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Qingdao Jiarui Biological Technology Co Ltd
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Abstract

The present invention discloses a kind of establishing-Yang Composite fermentation type Epimedium health wine and production technique thereof, this invention selects Herba Epimedii, hawthorn, bark of eucommia W-Gum to be that raw material prepares Epimedium health wine by complex ferment production technique, this product vinosity gentleness is tasty and refreshing, unique flavor, be of high nutritive value, natural nutrient component in basic maintenance Herba Epimedii, and be rich in the icarin of needed by human body, flavones, multiple amino acids, VITAMIN and mineral substance, there is kidney-replenishing, the effect such as the stasis of blood, reducing blood-fat is fallen apart in strengthening the bones and muscles, wind-damp dispelling, food digesting stomach fortifying, promoting the circulation of qi.Advantage of the present invention is: one is the technique adopting ultrasonic-assisted extraction, and extracts the biologically active substance such as icarin, flavones by fermentable, and active substance extraction yield is high, improves the nutritive value of Epimedium health wine; Two is adopt extraction ofHerba Epimedii, the bark of eucommia, Sucus Crataegi and W-Gum saccharification liquid complex ferment, improves the trophic level of Epimedium health wine; Three is the technique adopting micro-pore-film filtration, and product clarity is high, degerming, good impurity removing effect, instead of traditional pasteurization, and product Middle nutrition component damages is little.

Description

A kind of establishing-Yang Composite fermentation type Epimedium health wine and production technique thereof
Technical field
The present invention relates to a kind of preparation method of establishing-Yang Composite fermentation type Epimedium health wine, belong to herbal health care field of food.
Background technology
Herba Epimedii ( herba Epimedii) be Berberidaceae per nnial herb, all there is distribution in provinces and regions such as China Shaanxi, Hubei.Herba Epimedii is classified as nourishing class herbal medicine apart from the Shennong's Herbal of modern more than 2,000 year; " Chinese Pharmacopoeia " (version in 2010) is pointed out, Herba Epimedii can " kidney-replenishing, strengthening the bones and muscles, wind-damp dispelling, rheumatic arthralgia soft for kidney yang deficiency, impotence and seminal emission, muscles and bones impotence etc. ".Modern nutriology research shows, Herba Epimedii contains the several physiological active substances such as flavonoid compound, polysaccharide, mineral substance, and wherein flavonoid compound is considered to the main functional component playing the health protection effect such as immunity moderation, preventing osteoporosis.Toxicologic study shows, Herba Epimedii does not have acute toxicity, cytotoxicity and genotoxicity, belongs to nontoxic level material.2002, Herba Epimedii was classified as the herbal medicine that can be used for protective foods by health ministry.China's conventional Herba Epimedii among the people makes health tea, but has no the report of the Herba Epimedii health care health promoting wine mass-producing market development.Exploitation Epimedium health wine, contributes to bringing this for the Herba Epimedii medicine food dual purpose plant having health value into daily life, for the scientific utilization of China's Herba Epimedii resource opens up new approach.
Hawthorn, has another name called Fruit of Pashi Pear, is rosaceous plant, and it is a kind of food is also a kind of medicine.Measure according to science, it is mainly containing the number of chemical such as flavonoid compound, unsaturated fatty acids composition.The function that it has food digesting stomach fortifying, the stasis of blood is fallen apart in promoting the circulation of qi, also has the effect of certain antiphlogistic antibacterial and reduction blood fat in addition.Therefore, hawthorn both had nutritive value, also had certain health care and pharmacological action, was a kind of dark ace-high suitable to people of all ages food.
In recent years, along with the raising day by day of standard of living, the health perception of people is more and more stronger, consumption and the purchasing power of health-product market also constantly increase, particularly herbal health care product contains extremely abundant nutritive ingredient, and not only wherein amino acid classes is complete, is rich in the various trace elements such as potassium, calcium, and VITAMIN, the dark favor by human consumer.Fermented type health promoting wine is not only containing the active nutrient component in vegetable Chinese herbal medicine, human body being had to health-care effect, and produce a large amount of various nutritive ingredients (as sugar, protein, essential amino acid etc.) during the fermentation, appropriateness is drunk and can be played good health-care effect, becomes the product of herbal health care field of food and even whole field of health care food most development prospect.
The present invention take Semen Cassiae as prescription basis, compatibility hawthorn, the bark of eucommia, be aided with W-Gum, high reactivity fermentation by saccharomyces cerevisiae is utilized to have the herbal medicine of health-care effect, prepare a kind of newly there is Herba Epimedii, hawthorn, the health promoting wine of the herbal health care effects such as the bark of eucommia, both Herba Epimedii was remained, hawthorn, the medical health care effect of the herbal medicine such as the bark of eucommia, expand the application approach of Chinese herbal health care wine, maintain again the sweet-smelling of wine, a kind of nutritious, instant edible, the characteristic Chinese herbal health care wine of unique flavor, be applicable to multiple drunk by people, meet people to green, the demand of safe Yang-establishing health food.
Summary of the invention
The object of the present invention is to provide a kind of establishing-Yang Composite fermentation type Epimedium health wine and production technique thereof, this invention selects Herba Epimedii, hawthorn, bark of eucommia W-Gum to be that raw material prepares Epimedium health wine by complex ferment production technique, this product vinosity gentleness is tasty and refreshing, unique flavor, be of high nutritive value, natural nutrient component in basic maintenance Herba Epimedii, and be rich in the icarin of needed by human body, flavones, multiple amino acids, VITAMIN and mineral substance, there is kidney-replenishing, the effect such as the stasis of blood, reducing blood-fat is fallen apart in strengthening the bones and muscles, wind-damp dispelling, food digesting stomach fortifying, promoting the circulation of qi.
For achieving the above object, the technical solution used in the present invention is:
The invention provides a kind of establishing-Yang Composite fermentation type Epimedium health wine, it is characterized in that: establishing-Yang Composite fermentation type Epimedium health wine is obtained by high reactivity fermentation by saccharomyces cerevisiae composite fermentation liquid.
Above-mentioned composite fermentation liquid is the mixed solution of extraction ofHerba Epimedii, bark of eucommia extracting solution, Sucus Crataegi and W-Gum saccharification liquid, and each component proportion is: extraction ofHerba Epimedii 18 ~ 22%, Sucus Crataegi 10 ~ 20%, W-Gum saccharification liquid 50 ~ 56%, bark of eucommia extracting solution 9 ~ 15%.
Described extraction ofHerba Epimedii, bark of eucommia extracting solution prepare by following method: it is 7.5 ~ 8.0(Na that Herba Epimedii, the bark of eucommia dry product after pulverizing is dipped in pH 2cO 3regulate) pure water in, solid-liquid ratio is 1:3 ~ 5; Lixiviate 20 ~ 30min under ultrasonic assistant condition, extraction temperature 60 ~ 70 DEG C, namely obtains extraction ofHerba Epimedii and bark of eucommia extracting solution after lixiviate terminates.
Described Sucus Crataegi is prepared by following method: take a certain amount of hawthorn, adds the water of 10 times, puts into 75 DEG C of water-baths and soaks 2 ~ 3h, then pull an oar.
Described W-Gum saccharification liquid is prepared in following method: join in pure water in the ratio of 30 ~ 35g/100mL by W-Gum, stirs homogenate, adds the α-amylase of 2%, and 60 ~ 70 DEG C of liquefaction 20 ~ 30min, obtain thick shape white liquid; Then temperature control saccharification under constant temperature, saccharification pH controls 4.5 ~ 5.5, and saccharifying enzyme add-on is 1.5 ~ 2.0%, and saccharification temperature controls amylase optimum temperature 60 DEG C, and saccharification time is 2 ~ 2.5h, filters to obtain W-Gum saccharification liquid.
The invention provides a kind of establishing-Yang Composite fermentation type Epimedium health wine, it is characterized in that preparing by following method:
1) composite fermentation liquid allotment: extraction ofHerba Epimedii, Sucus Crataegi, W-Gum saccharification liquid and bark of eucommia extracting solution are mixed in proportion, adds 0.12% (NH 4) 2sO 4, 0.10% K 2hPO 4, 0.1%MgSO 4, with about W-Gum saccharification liquid adjustment total reducing sugar to 12%;
2) actication of culture: high reactivity Wine-making yeast is activated 30min in 30 ~ 40 DEG C of warm water;
3) inoculate: by the bacterial classification access fermented liquid after activation, inoculative proportion is 6 ~ 8%;
4) temperature controlled fermentation: temperature controlled fermentation under slow stirring, leavening temperature controls at 26 ~ 28 DEG C, and fermentation time is 36 ~ 48h;
5) filter: passed through after fermentation ends to filter the solid insoluble such as Chinese herb residues and fermentation thalli;
6) ageing: for promoting the stable and clarification just proposed, to be placed under 0 ~ 10 DEG C of condition ageing 3 ~ 5 days by wine liquid;
7) allocate: the alcoholic strength 10% ~ 12% of adjustment Epimedium health wine/( v/v), add 1 ~ 1.5g/L Xylitol, 3 ~ 5g/L potassium sorbate, 1 ~ 2g/L Tripotassium Citrate, 5 ~ 10g/L oligomeric isomaltose;
8) membrane filtration: the micro-pore-film filtration Epimedium health wine with aperture being 0.15 μm;
9) sterile filling: filling and sealing obtains Composite fermentation type Epimedium health wine in sterile filling production line.
The invention provides a kind of establishing-Yang Composite fermentation type Epimedium health wine and production technique thereof, in technique, α-amylase used, saccharifying enzyme are the delicatessen food level product that letter (Denmark) Bioisystech Co., Ltd of Novi produces; Fermented yeast used is commercially available Angel TH Active Dry Yeast.
The invention provides a kind of establishing-Yang Composite fermentation type Epimedium health wine and production technique thereof, wherein the product feature of Epimedium health wine be that the wine degree of Epimedium health wine is 10% ~ 12%/( v/v), sugar degree≤4 g/L, total acid (in oxysuccinic acid) 5 ~ 8 g/L, volatile acid (with acetometer) < 1.2 g/L, sugar-free extract>=17 g/L; Epimedium health wine color and luster is red-brown, crystal clear, no suspended substance and throw out; Entrance is soft pure and honest, comfortable tasty and refreshing, and all tastes are coordinated.
The invention provides a kind of establishing-Yang Composite fermentation type Epimedium health wine and production technique thereof, wherein the product of Epimedium health wine has the effects such as kidney-replenishing, strengthening the bones and muscles, wind-damp dispelling; Transformation Application of the present invention not only can change drinks consumption result, meet the people to demand that is green, safe Yang-establishing health food, be of value to national health, and high-valuedly can make full use of Chinese herbal medicine resource, increase the income of the herbal medicine growing area people, drive farmer richness.
The invention provides a kind of establishing-Yang Composite fermentation type Epimedium health wine and production technique thereof, it is advantageous that: one is the technique adopting ultrasonic-assisted extraction, and by fermentable effect, extract the biologically active substances such as icarin, flavones, VITAMIN, improve the extraction yield of active substance, improve the nutritive value of Epimedium health wine; Two is adopt extraction ofHerba Epimedii, the bark of eucommia, Sucus Crataegi and W-Gum saccharification liquid complex ferment, both ensure that the sugared content in Preliminary fermentation liquid, and has turn improved the trophic level of Epimedium health wine; Three is adopt complex ferment technique, compares health promoting wine and blends production technique and have more advantage, product taste and trophic level higher; Four is the production technique adopting micro-pore-film filtration, and product clarity is high, degerming, good impurity removing effect, and sterilising effect is thorough, and instead of traditional pasteurization, product Middle nutrition component damages is little.
Embodiment
embodiment 1:
Prepare Epimedium health wine with Herba Epimedii, the bark of eucommia, hawthorn and W-Gum complex ferment, its concrete production technique is as follows:
1) extraction ofHerba Epimedii, the preparation of bark of eucommia extracting solution: it is 7.5 ~ 8.0(Na that Herba Epimedii, the bark of eucommia dry product after pulverizing is dipped in pH 2cO 3regulate) pure water in, solid-liquid ratio is 1:3; Lixiviate 20 ~ 30min under ultrasonic assistant condition, extraction temperature 60 ~ 70 DEG C, namely obtains extraction ofHerba Epimedii and bark of eucommia extracting solution after lixiviate terminates;
2) Sucus Crataegi preparation: take a certain amount of hawthorn, broken after cleaning, add the water of 10 times, put into 75 DEG C of water-baths and soak 2 ~ 3h, then pull an oar;
3) W-Gum saccharification liquid preparation: join in pure water by W-Gum in the ratio of 30g/100mL, stirs homogenate, adds the α-amylase of 2%, and 60 ~ 70 DEG C of liquefaction 20 ~ 30min, obtain thick shape white liquid; Then temperature control saccharification under constant temperature, saccharification pH controls 4.5 ~ 5.5, and saccharifying enzyme add-on is 1.5 ~ 2.0%, and saccharification temperature controls amylase optimum temperature 60 DEG C, and saccharification time is 2 ~ 2.5h, filters to obtain W-Gum saccharification liquid;
5) composite fermentation liquid allotment: extraction ofHerba Epimedii, Sucus Crataegi, W-Gum saccharification liquid and bark of eucommia extracting solution are mixed in 22%, 10%, 56%, 12% ratio, adds 0.12% (NH 4) 2sO 4, 0.10% K 2hPO 4, 0.1%MgSO 4, with about W-Gum saccharification liquid adjustment total reducing sugar to 12%;
6) actication of culture: high reactivity Wine-making yeast is activated 30min in 30 ~ 40 DEG C of warm water;
7) inoculate: by the bacterial classification access fermented liquid after activation, inoculative proportion is 8%;
8) temperature controlled fermentation: temperature controlled fermentation under slow stirring, leavening temperature controls at 26 ~ 28 DEG C, and fermentation time is 48h;
9) filter: passed through after fermentation ends to filter the solid insoluble such as Chinese herb residues and fermentation thalli;
10) ageing: for promoting the stable and clarification just proposed, to be placed under 0 ~ 10 DEG C of condition ageing 5 days by wine liquid;
11) allocate: the alcoholic strength 10% ~ 12% of adjustment Epimedium health wine/( v/v), add 1.5g/L Xylitol, 3g/L potassium sorbate, 2g/L Tripotassium Citrate, 5g/L oligomeric isomaltose;
12) membrane filtration: the micro-pore-film filtration Epimedium health wine with aperture being 0.15 μm;
13) sterile filling: filling and sealing obtains Composite fermentation type Epimedium health wine in sterile filling production line.
embodiment 2:
Prepare Epimedium health wine with Herba Epimedii, the bark of eucommia, hawthorn and W-Gum complex ferment, its concrete production technique is as follows:
1) extraction ofHerba Epimedii, the preparation of bark of eucommia extracting solution: it is 7.5 ~ 8.0(Na that Herba Epimedii, the bark of eucommia dry product after pulverizing is dipped in pH 2cO 3regulate) pure water in, solid-liquid ratio is 1:4; Lixiviate 20 ~ 30min under ultrasonic assistant condition, extraction temperature 60 ~ 70 DEG C, namely obtains extraction ofHerba Epimedii and bark of eucommia extracting solution after lixiviate terminates;
2) Sucus Crataegi preparation: take a certain amount of hawthorn, broken after cleaning, add the water of 10 times, put into 75 DEG C of water-baths and soak 2h ~ 3h, then pull an oar;
3) W-Gum saccharification liquid preparation: join in pure water by W-Gum in the ratio of 35g/100mL, stirs homogenate, adds the α-amylase of 2%, and 60 ~ 70 DEG C of liquefaction 20 ~ 30min, obtain thick shape white liquid; Then temperature control saccharification under constant temperature, saccharification pH controls 4.5 ~ 5.5, and saccharifying enzyme add-on is 1.5 ~ 2.0%, and saccharification temperature controls amylase optimum temperature 60 DEG C, and saccharification time is 2 ~ 2.5h, filters to obtain W-Gum saccharification liquid;
5) composite fermentation liquid allotment: extraction ofHerba Epimedii, Sucus Crataegi, W-Gum saccharification liquid and bark of eucommia extracting solution are mixed in 18%, 20%, 50%, 12% ratio, adds 0.12% (NH 4) 2sO 4, 0.10% K 2hPO 4, 0.1%MgSO 4, with about W-Gum saccharification liquid adjustment total reducing sugar to 12%;
6) actication of culture: high reactivity Wine-making yeast is activated 30min in 30 ~ 40 DEG C of warm water;
7) inoculate: by the bacterial classification access fermented liquid after activation, inoculative proportion is 7%;
8) temperature controlled fermentation: temperature controlled fermentation under slow stirring, leavening temperature controls at 26 ~ 28 DEG C, and fermentation time is 42h;
9) filter: passed through after fermentation ends to filter the solid insoluble such as Chinese herb residues and fermentation thalli;
10) ageing: for promoting the stable and clarification just proposed, to be placed under 0 ~ 10 DEG C of condition ageing 3 ~ 5 days by wine liquid;
11) allocate: the alcoholic strength 10% ~ 12% of adjustment Epimedium health wine/( v/v), add 1g/L Xylitol, 5g/L potassium sorbate, 1g/L Tripotassium Citrate, 10g/L oligomeric isomaltose;
12) membrane filtration: the micro-pore-film filtration Epimedium health wine with aperture being 0.15 μm;
13) sterile filling: filling and sealing obtains Composite fermentation type Epimedium health wine in sterile filling production line.
embodiment 3:
Prepare Epimedium health wine with Herba Epimedii, the bark of eucommia, hawthorn and W-Gum complex ferment, its concrete production technique is as follows:
1) extraction ofHerba Epimedii, the preparation of bark of eucommia extracting solution: it is 7.5 ~ 8.0(Na that Herba Epimedii, the bark of eucommia dry product after pulverizing is dipped in pH 2cO 3regulate) pure water in, solid-liquid ratio is 1:5; Lixiviate 20 ~ 30min under ultrasonic assistant condition, extraction temperature 60 ~ 70 DEG C, namely obtains extraction ofHerba Epimedii and bark of eucommia extracting solution after lixiviate terminates;
2) Sucus Crataegi preparation: take a certain amount of hawthorn, broken after cleaning, add the water of 10 times, put into 75 DEG C of water-baths and soak 2h ~ 3h, then pull an oar;
3) W-Gum saccharification liquid preparation: join in pure water by W-Gum in the ratio of 32g/100mL, stirs homogenate, adds the α-amylase of 2%, and 60 ~ 70 DEG C of liquefaction 20 ~ 30min, obtain thick shape white liquid; Then temperature control saccharification under constant temperature, saccharification pH controls 4.5 ~ 5.5, and saccharifying enzyme add-on is 1.5 ~ 2.0%, and saccharification temperature controls amylase optimum temperature 60 DEG C, and saccharification time is 2 ~ 2.5h, filters to obtain W-Gum saccharification liquid;
5) composite fermentation liquid allotment: extraction ofHerba Epimedii, Sucus Crataegi, W-Gum saccharification liquid and bark of eucommia extracting solution are mixed in 20%, 15%, 53%, 12% ratio, adds 0.12% (NH 4) 2sO 4, 0.10% K 2hPO 4, 0.1%MgSO 4, with about W-Gum saccharification liquid adjustment total reducing sugar to 12%;
6) actication of culture: high reactivity Wine-making yeast is activated 30min in 30 ~ 40 DEG C of warm water;
7) inoculate: by the bacterial classification access fermented liquid after activation, inoculative proportion is 7%;
8) temperature controlled fermentation: temperature controlled fermentation under slow stirring, leavening temperature controls at 26 ~ 28 DEG C, and fermentation time is 42h;
9) filter: passed through after fermentation ends to filter the solid insoluble such as Chinese herb residues and fermentation thalli;
10) ageing: for promoting the stable and clarification just proposed, to be placed under 0 ~ 10 DEG C of condition ageing 3 ~ 5 days by wine liquid;
11) allocate: the alcoholic strength 10% ~ 12% of adjustment Epimedium health wine/( v/v), add 1.2g/L Xylitol, 4g/L potassium sorbate, 1.5g/L Tripotassium Citrate, 8g/L oligomeric isomaltose;
12) membrane filtration: the micro-pore-film filtration Epimedium health wine with aperture being 0.15 μm;
13) sterile filling: filling and sealing obtains Composite fermentation type Epimedium health wine in sterile filling production line.
Above embodiment only for illustration of technical scheme of the present invention, but not is limited; Although be described in detail by invention with reference to previous embodiment, for the person of ordinary skill of the art, still the technical scheme described in previous embodiment can be modified, or equivalent replacement has been carried out to wherein portion of techniques feature; And to these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of the present invention's technical scheme required for protection.

Claims (4)

1. an establishing-Yang Composite fermentation type Epimedium health wine, is characterized in that being prepared by following steps:
1) composite fermentation liquid allotment: in extraction ofHerba Epimedii 18 ~ 22%, Sucus Crataegi 10 ~ 20%, W-Gum saccharification liquid 50 ~ 56%, the mixing of bark of eucommia extracting solution 9 ~ 15% ratio, each component sum is 100%, adds 0.12% (NH 4) 2sO 4, 0.10%K 2hPO 4, 0.1%MgSO 4, form sediment with corn
2) actication of culture: high reactivity Wine-making yeast is activated 30min in 30 ~ 40 DEG C of warm water;
3) inoculate: by the bacterial classification access fermented liquid after activation, inoculative proportion is 6 ~ 8%;
4) temperature controlled fermentation: temperature controlled fermentation under slow stirring, leavening temperature controls at 26 ~ 28 DEG C, and fermentation time is 36 ~ 48h;
5) filter: by solids removed by filtration insolubles Chinese herb residues and fermentation thalli after fermentation ends;
tripotassium Citrate, 5 ~ 10g/L oligomeric isomaltose;
8) membrane filtration: the micro-pore-film filtration Epimedium health wine with aperture being 0.15 μm;
9) sterile filling: filling and sealing obtains Composite fermentation type Epimedium health wine in sterile filling production line.
2. a kind of establishing-Yang Composite fermentation type Epimedium health wine according to claim 1, is characterized in that described extraction ofHerba Epimedii, bark of eucommia extracting solution prepares as follows: the Herba Epimedii after pulverizing, bark of eucommia dry product are dipped in and use Na 2cO 3regulate pH to be in the pure water of 7.5 ~ 8.0, solid-liquid ratio is 1:3 ~ 5; Lixiviate 20 ~ 30min under ultrasonic assistant condition, extraction temperature 60 ~ 70 DEG C, namely obtains extraction ofHerba Epimedii and bark of eucommia extracting solution after lixiviate terminates.
3. a kind of establishing-Yang Composite fermentation type Epimedium health wine according to claim 1, is characterized in that described Sucus Crataegi is prepared as follows: take a certain amount of hawthorn, adds the water of 10 times, puts into 75 DEG C of water-baths and soaks 2 ~ 3h, then pull an oar.
4. a kind of establishing-Yang Composite fermentation type Epimedium health wine according to claim 1, it is characterized in that described W-Gum saccharification liquid is prepared in following method: join in pure water in the ratio of 30 ~ 35g/100mL by W-Gum, stir homogenate, add the α-amylase of 2%, 60 ~ 70 DEG C of liquefaction 20 ~ 30min, obtain thick shape white liquid; Then temperature control saccharification under constant temperature, saccharification pH controls 4.5 ~ 5.5, and saccharifying enzyme add-on is 1.5 ~ 2.0%, and saccharification temperature controls amylase optimum temperature 60 DEG C, and saccharification time is 2 ~ 2.5h, filters to obtain W-Gum saccharification liquid.
CN201310604612.7A 2013-11-26 2013-11-26 A kind of establishing-Yang Composite fermentation type Epimedium health wine and production technique thereof Expired - Fee Related CN103642658B (en)

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CN101397531A (en) * 2008-05-08 2009-04-01 黄迪南 Oyster wine for replenishing vital essence and invigorating vital function and its preparing process
CN102260616A (en) * 2010-05-28 2011-11-30 周凤英 Wine capable of drawing attention and tonifying kidney

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Publication number Priority date Publication date Assignee Title
CN1388243A (en) * 2001-05-30 2003-01-01 中国科学院微生物研究所 One brewer's yeast engineering saccharomycete strain and the production process of alcohol and ergosterin with the strain
CN101397531A (en) * 2008-05-08 2009-04-01 黄迪南 Oyster wine for replenishing vital essence and invigorating vital function and its preparing process
CN102260616A (en) * 2010-05-28 2011-11-30 周凤英 Wine capable of drawing attention and tonifying kidney

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