CN103583606A - Root-knot nematode control agent as well as preparing method and application thereof - Google Patents
Root-knot nematode control agent as well as preparing method and application thereof Download PDFInfo
- Publication number
- CN103583606A CN103583606A CN201310601194.6A CN201310601194A CN103583606A CN 103583606 A CN103583606 A CN 103583606A CN 201310601194 A CN201310601194 A CN 201310601194A CN 103583606 A CN103583606 A CN 103583606A
- Authority
- CN
- China
- Prior art keywords
- weight portion
- root
- knot nematode
- control agent
- nematode control
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a root-knot nematode control agent as well as a preparing method and application thereof, belongs to the field of agriculture, and aims to solve the technical problem that a plant source root-knot nematode control agent as well as a preparing method and application thereof are provided. The root-knot nematode control agent provided by the invention has active constituents prepared from the following plant materials of Chinese herbal medicines in parts by weight: 1-8 parts of peganum harmala, 0-8 parts of folium ginkgo, 1-10 parts of sphora alopecuroide, 0-5 parts of radices sophorae subprostratae, 0-5 parts of rhizoma menispermi, 0-10 parts of radix sophorae flavescentis, 0-8 parts of castor beans, 1-10 parts of abutilon fibers, 0-8 parts of crown daisy and 0-5 parts of mandala. Meanwhile, the invention further discloses a preparing method and application of the root-knot nematode control agent. The root-knot nematode compound has the advantages of safety and nontoxicity, meanwhile can effectively prevent, restrain and kill root-knot nematodes.
Description
Technical field
The present invention relates to root knot nematode control agent and its preparation method and application, belong to agriculture field.
Background technology
Root knot nematode disease is the multiple Vegetables such as one of main plant nematode disease, main harm cucumber, balsam pear, tomato, eggplant, Kidney bean, celery, spinach.Adjustment along with crop mix; the rapid expansion of vegetable protecting field area under cultivation; root-knot nematode is pathogenetic increases the weight of year by year; production loss reaches 30%～50%; ruin seedling heavily the event of planting happen occasionally; in addition, root-knot nematode harm has also increased the weight of the generation of the native transmissibility fungal diseases such as fusarium wilt, root rot and parts of fine fungus diseases, has become the large obstacle that current vegetables produce.
Chemical control is still the topmost prophylactico-therapeutic measures of plant nematode disease, but the use cost of traditional chemical nematode killing agents such as chloropicrin, Celfume, carbofuran, Nemacur, Methomyl is high, and Environmental compatibility is poor.The biological control method control root-knot nematode that application has operability is the common recognition of Chinese scholars, and studying, develop, apply new and effective plant resource GR is the trend of root knot nematode control development.There are at present many R&D institutions to carry out the research of this respect, but all do not obtain the medicament of desirable preventive effect, therefore work out and utilize plant seed or leaf, cane, through extracting, obtain efficient, safe plant resource GR, and the production that is applicable to organic farming, green agriculture is problem demanding prompt solution.
Summary of the invention
The technical problem to be solved in the present invention is that a kind of plant resource root knot nematode control agent and its preparation method and application is provided.
Root knot nematode control agent of the present invention, its active component is prepared from by the raw material of following weight portion: the Tong fiber crops of the ginkgo leaf of the peganum harmala L of 1～8 weight portion, 0～8 weight portion, the Sophora alopecuroide of 1～10 weight portion, the root of subprostrate sophora of 0～5 weight portion, the kuh-seng of the asiatic moonseed of 0～5 weight portion, 0～10 weight portion, the castor bean of 0～8 weight portion, 1～10 weight portion, the garland chrysanthemum of 0～8 weight portion are, the datura of 0～5 weight portion.
Further, as preferred version, the active component of root knot nematode control agent of the present invention is prepared from by the raw material of following weight portion:
The Tong fiber crops of the castor bean of the kuh-seng of the asiatic moonseed of the root of subprostrate sophora of the Sophora alopecuroide of the ginkgo leaf of the peganum harmala L of 1～8 weight portion, 0.5～8 weight portion, 1～10 weight portion, 0～5 weight portion, 0～5 weight portion, 0～10 weight portion, 0～8 weight portion, 1～10 weight portion, the garland chrysanthemum of 3～8 weight portions are, the datura of 1～5 weight portion;
Or the peganum harmala L of 1～5 weight portion, the Sophora alopecuroide of 1～6 weight portion are, the Tong of 1～8 weight portion is numb, the ginkgo leaf of 1～7 weight portion is, the castor bean of the kuh-seng of 1～5 weight portion, 1～8 weight portion;
Or the peganum harmala L of 1～5 weight portion, the Sophora alopecuroide of 1～5 weight portion, the castor bean of the piemarker of 1～5 weight portion, 0.5～6 weight portion and the ginkgo leaf of 1～6 weight portion;
Or the Tong of the peganum harmala L of 2～5 weight portions, 2～5 weight portions fiber crops, the Sophora alopecuroide of 2～5 weight portions and the castor bean of 2～5 weight portions.
Wherein, above-mentioned raw materials is Chinese herbal medicine raw material, described active component can directly be pulverized said herbal medicine plant material or become thoroughly decomposed, fermenting is prepared from, in order to improve the effect of root knot nematode control agent of the present invention, as preferred version, its active component is got and is prepared from through ethanolic solution or water extraction by said herbal medicine plant material.
Further, in order to improve the activity of root knot nematode control agent, in described root knot nematode control agent, except active component, also preferably contain auxiliary material, described auxiliary material can adopt the conventional auxiliary material in composition, such as adopt in dispersant, wetting agent, bleeding agent, synergist at least one; As preferred version, described bleeding agent is at least one in fatty alcohol polyethenoxy ether class surfactant, organosilicon bleeding agent, Organic fluoride bleeding agent; Described dispersant is at least one in lignosulfonates, sldium lauryl sulfate, phosphate, alkylphenol-polyethenoxy base phosphate; Described wetting agent is at least one in alkyl sulfate, alkylbenzenesulfonate, alkylnaphthalene sulfonate, aliphatic alcohol polyethenoxy base ether, alkylphenol-polyethenoxy base ether, sorbitan fatty acid ester; Described synergist is organosilicon.Root knot nematode control agent of the present invention can be prepared into the common formulations such as aqua, pulvis or granule.
Further, in described pulvis and granule, also comprise carrier, the preferred maltose of described carrier, soluble starch or fertilizer element; Wherein, at least one in described fertilizer element preferably sulfuric acid potassium, ammonium sulfate, MAP, diammonium phosphate, magnesium sulfate, potassium dihydrogen phosphate or organic matter; Organic is agricultural crop straw at least one that thing, careless carbon, ight soil become thoroughly decomposed in thing etc. of becoming thoroughly decomposed.
Wherein, described root knot nematode control agent can adopt conventional method preparation, such as adopting in active component, add auxiliary material and carrier, stir, be dried, pulverize and to obtain pulvis, also can after adding filler drying to make powder in active component, prepare again, mix, be dried, pulverize and to obtain powder product.The preparation method of granule is: by active component mix rear granulation with filler, dispersant, wetting agent, synergist etc., be dried, sieve (1～4.75um), obtain granule.
Further, in order to improve the effect of root knot nematode control agent, root knot nematode control agent of the present invention preferably adopts following three kinds of methods preparation and obtains:
Method (one):
A, pulverizing: Chinese herbal medicine raw material is mixed and pulverized;
B, immersion: the Chinese herbal medicine raw material after the pulverizing of a step is added in solvent and soaked, obtain immersion mixture, soak time preferably 12～36 hours; Wherein, described solvent is ethanol water, preferred 1:20～50 of mass ratio of described Chinese herbal medicine raw material and solvent, the preferred 0.25～4:1 of the mass ratio of water and ethanol in solvent;
C, extraction: b step gained immersion mixture is preferable over to ultrasonic lixiviate 30～60min under 20～40 ℃ of conditions, obtains extract;
D, preparation: extract is made to aqua, pulvis or granule.
Wherein, in order to improve the production efficiency of root knot nematode control agent, reduce production costs, soak time more preferably 18～30 hours, the mass ratio of Chinese herbal medicine raw material and solvent is 1:30～40 more preferably preferably, the further preferred 0.5～1:1 of the mass ratio of water and ethanol in solvent.
Wherein, for the ease of storage and transport, can extract is concentrated, obtain concentrate or medicinal extract, and then be prepared into various formulations; Cycles of concentration can be adjusted voluntarily according to needs of production.
Method (two):
A, pulverizing: Chinese herbal medicine raw material is mixed and pulverized;
B, preparation: the raw material after a step is pulverized is made pulvis.
Method (three):
A, fermentation maturity: by Chinese herbal medicine raw material hybrid concurrency ferment or become thoroughly decomposed;
B, preparation: the raw material after becoming thoroughly decomposed is by fermentation made to pulvis or granule;
Wherein, active in order to improve root knot nematode control agent of the present invention, as preferred version, after Chinese herbal medicine raw material is pulverized, through row, ferment or become thoroughly decomposed again.
Further, the invention also discloses the application of root knot nematode control agent in control plant root-knot nematode.In order to improve the effect of root knot nematode control agent, the use age is preferably the seedling stage of plant; Root knot nematode control agent of the present invention can be used by common method, and as poured into a mould, spray, spraying, dusting, distribution, its amount of application is with weather condition or crop state variation.General, adopt the methods such as cast, spraying, while using by root knot nematode control agent thin up of the present invention to suitable concentration, concentration can regulate according to needs of production, amount of application preferably 2～8L/ mu/time.Preferably aqua is adopted and is sprayed on plant blade face, more preferably when plant premorbid or their early stage, by spray pouring crop root soil around doubly after aqua dilution, carry out secondary dispenser after being preferable over 10～15 days, the preferred continuous administration of one season crop 2～3 times, amount of application is 3～8L/ mu.For pulvis or granule, except being watered rear pouring, spraying by above-mentioned extension rate, rewater after also can spreading fertilizer over the fields or mix soil, make active ingredient can be distributed to as much as possible the place that crop root can reach.Also can before and after transplanting, crops spread fertilizer over the fields, scatter, mix soil to the soil of crop, make worm's ovum or larva suppressed or kill, amount of application is also by 2～8 kgs/acre times, at Seedling Stage, by 2～4 kgs/acre times, use, occurring degree and insect density with root-knot nematode after 10～15 days are determined consumption 4-8 kg/acre time.
Root knot nematode control agent of the present invention can be widely used in can being subject to preventing and treating the plant of root-knot nematode disease, be that root knot nematode control agent of the present invention can be for prevention plant root-knot nematode evil, or for being subject to the plant of root-knot nematode evil, the common plant that is subject to root-knot nematode evil has water spinach, cucumber, tomato, carrot, Chinese cabbage, lettuce, eggplant, custard squash etc.
Beneficial effect of the present invention:
1, safety, easily degraded are used in root knot nematode control agent of the present invention, to human body and environmentally friendly, also can not cause the residual of hormone, can not bring safety problem.
2, root knot nematode control agent of the present invention has good effect to control root-knot nematode, and the biological composition higher than conventional, is not less than Chemical composition that.
3, root knot nematode control agent of the present invention can also obviously improve the nutrition condition of crop simultaneously, promotes growing of crop, improves its immunity.
4, root knot nematode control agent of the present invention contain enrich polysaccharide, amino acid, etc. organic matter, provide microorganism required organic matter, optimize microbial environment, improvement fertilizing soil.
5, in the present invention, root knot nematode control agent is with low cost, and preparation method is simple, without dropping into large-scale special equipment.
Embodiment
Root knot nematode control agent of the present invention, its active component is prepared from by the raw material of following weight portion: the Tong fiber crops of the ginkgo leaf of the peganum harmala L of 1～8 weight portion, 0～8 weight portion, the Sophora alopecuroide of 1～10 weight portion, the root of subprostrate sophora of 0～5 weight portion, the kuh-seng of the asiatic moonseed of 0～5 weight portion, 0～10 weight portion, the castor bean of 0～8 weight portion, 1～10 weight portion, the garland chrysanthemum of 0～8 weight portion are, the datura of 0～5 weight portion.
Further, as preferred version, the active component of root knot nematode control agent of the present invention is prepared from by the raw material of following weight portion:
The Tong fiber crops of the castor bean of the kuh-seng of the asiatic moonseed of the root of subprostrate sophora of the Sophora alopecuroide of the ginkgo leaf of the peganum harmala L of 1～8 weight portion, 0.5～8 weight portion, 1～10 weight portion, 0～5 weight portion, 0～5 weight portion, 0～10 weight portion, 0～8 weight portion, 1～10 weight portion, the garland chrysanthemum of 3～8 weight portions are, the datura of 1～5 weight portion;
Or the peganum harmala L of 1～5 weight portion, the Sophora alopecuroide of 1～6 weight portion are, the Tong of 1～8 weight portion is numb, the ginkgo leaf of 1～7 weight portion is, the castor bean of the kuh-seng of 1～5 weight portion, 1～8 weight portion;
Or the peganum harmala L of 1～5 weight portion, the Sophora alopecuroide of 1～5 weight portion, the castor bean of the piemarker of 1～5 weight portion, 0.5～6 weight portion and the ginkgo leaf of 1～6 weight portion;
Or the Tong of the peganum harmala L of 2～5 weight portions, 2～5 weight portions fiber crops, the Sophora alopecuroide of 2～5 weight portions and the castor bean of 2～5 weight portions.
Wherein, above-mentioned raw materials is Chinese herbal medicine raw material, described active component can directly be pulverized said herbal medicine plant material or become thoroughly decomposed, fermenting is prepared from, in order to improve the effect of root knot nematode control agent of the present invention, as preferred version, its active component is got and is prepared from through ethanolic solution or water extraction by said herbal medicine plant material.
Further, in order to improve the activity of root knot nematode control agent, in described root knot nematode control agent, except active component, also preferably contain auxiliary material, described auxiliary material can adopt the conventional auxiliary material in composition, such as adopt in dispersant, wetting agent, bleeding agent, synergist at least one; As preferred version, described bleeding agent is at least one in fatty alcohol polyethenoxy ether class surfactant, organosilicon bleeding agent, Organic fluoride bleeding agent; Described dispersant is at least one in lignosulfonates, sldium lauryl sulfate, phosphate, alkylphenol-polyethenoxy base phosphate; Described wetting agent is at least one in alkyl sulfate, alkylbenzenesulfonate, alkylnaphthalene sulfonate, aliphatic alcohol polyethenoxy base ether, alkylphenol-polyethenoxy base ether, sorbitan fatty acid ester; Described synergist is organosilicon.Root knot nematode control agent of the present invention can be prepared into the common formulations such as aqua, pulvis or granule.
Further, in described pulvis and granule, also comprise carrier, the preferred maltose of described carrier, soluble starch or fertilizer element; Wherein, at least one in described fertilizer element preferably sulfuric acid potassium, ammonium sulfate, MAP, diammonium phosphate, magnesium sulfate, potassium dihydrogen phosphate or organic matter; Organic is agricultural crop straw at least one that thing, careless carbon, ight soil become thoroughly decomposed in thing etc. of becoming thoroughly decomposed.
Wherein, described root knot nematode control agent can adopt conventional method preparation, such as adopting in active component, add auxiliary material and carrier, stir, be dried, pulverize and to obtain pulvis, also can after adding filler drying to make powder in active component, prepare again, mix, be dried, pulverize and to obtain powder product.The preparation method of granule is: by active component mix rear granulation with filler, dispersant, wetting agent, synergist etc., be dried, sieve (1～4.75um), obtain granule.
Further, in order to improve the effect of root knot nematode control agent, root knot nematode control agent of the present invention preferably adopts following three kinds of methods preparation and obtains:
Method (one):
A, pulverizing: Chinese herbal medicine raw material is mixed and pulverized;
B, immersion: the Chinese herbal medicine raw material after the pulverizing of a step is added in solvent and soaked, obtain immersion mixture, soak time preferably 12～36 hours; Wherein, described solvent is ethanol water, preferred 1:20～50 of mass ratio of described Chinese herbal medicine raw material and solvent, the preferred 0.25～4:1 of the mass ratio of water and ethanol in solvent;
C, extraction: b step gained immersion mixture is preferable over to ultrasonic lixiviate 30～60min under 20～40 ℃ of conditions, obtains extract;
D, preparation: extract is made to aqua, pulvis or granule.
Wherein, in order to improve the production efficiency of root knot nematode control agent, reduce production costs, soak time more preferably 18～30 hours, the mass ratio of Chinese herbal medicine raw material and solvent is 1:30～40 more preferably preferably, the further preferred 0.5～1:1 of the mass ratio of water and ethanol in solvent.
Wherein, for the ease of storage and transport, can extract is concentrated, obtain concentrate or medicinal extract, and then be prepared into various formulations; Cycles of concentration can be adjusted voluntarily according to needs of production.
Method (two):
A, pulverizing: Chinese herbal medicine raw material is mixed and pulverized;
B, preparation: the raw material after a step is pulverized is made pulvis.
Method (three):
A, fermentation maturity: by Chinese herbal medicine raw material hybrid concurrency ferment or become thoroughly decomposed;
B, preparation: the raw material after becoming thoroughly decomposed is by fermentation made to pulvis or granule;
Wherein, active in order to improve root knot nematode control agent of the present invention, as preferred version, after Chinese herbal medicine raw material is pulverized, through row, ferment or become thoroughly decomposed again.
Further, the invention also discloses the application of root knot nematode control agent in control plant root-knot nematode.In order to improve the effect of root knot nematode control agent, the use age is preferably the seedling stage of plant; Root knot nematode control agent of the present invention can be used by common method, and as poured into a mould, spray, spraying, dusting, distribution, its amount of application is with weather condition or crop state variation.General, adopt the methods such as cast, spraying, while using by root knot nematode control agent thin up of the present invention to suitable concentration, concentration can regulate according to needs of production, amount of application preferably 2～8L/ mu/time.Preferably aqua is adopted and is sprayed on plant blade face, more preferably when plant premorbid or their early stage, by spray pouring crop root soil around doubly after aqua dilution, carry out secondary dispenser after being preferable over 10～15 days, the preferred continuous administration of one season crop 2～3 times, amount of application is 3～8L/ mu.For pulvis or granule, except being watered rear pouring, spraying by above-mentioned extension rate, rewater after also can spreading fertilizer over the fields or mix soil, make active ingredient can be distributed to as much as possible the place that crop root can reach.Also can before and after transplanting, crops spread fertilizer over the fields, scatter, mix soil to the soil of crop, make worm's ovum or larva suppressed or kill, amount of application is also by 2～8 kgs/acre times, at Seedling Stage, by 2～4 kgs/acre times, use, occurring degree and insect density with root-knot nematode after 10～15 days are determined consumption 4-8 kg/acre time.
Root knot nematode control agent of the present invention can be widely used in can being subject to preventing and treating the plant of root-knot nematode disease, be that root knot nematode control agent of the present invention can be for prevention plant root-knot nematode evil, or for being subject to the plant of root-knot nematode evil, the common plant that is subject to root-knot nematode evil has water spinach, cucumber, tomato, carrot, Chinese cabbage, lettuce, eggplant, custard squash etc.
Below in conjunction with embodiment, the specific embodiment of the present invention is further described, does not therefore limit the present invention among described scope of embodiments.
The preparation of embodiment 1 root knot nematode control agent of the present invention
A, pulverizing: raw material is mixed and pulverized according to the herbal raw material component in table 1;
B, immersion: the herbal raw material after pulverizing is added in ethanol water and soaked, obtain immersion mixture, wherein, herbal raw material and solvent proportion, ethanol water concentration are in Table 2;
C, extraction: by the ultrasonic lixiviate of b step gained immersion mixture, obtain, extraction conditions is in Table 3;
D, preparation: extract is made to aqua, pulvis or granule.
Table 1 is respectively organized raw material and proportioning thereof
| Numbering | Component and quality thereof |
| gh-01 | Peganum harmala L: Sophora alopecuroide: piemarker: castor bean: ginkgo leaf=10g:10g:10g:5g:10g |
| gh-02 | Peganum harmala L: Sophora alopecuroide: piemarker: castor bean: ginkgo leaf=30g:30g:30g:35g:30g |
| gh-03 | Peganum harmala L: Sophora alopecuroide: piemarker: castor bean: ginkgo leaf=50g:50g:50g:60g:60g |
| gh-04 | Peganum harmala L: Tong fiber crops: Sophora alopecuroide: castor bean=20g:20g:50g:30g |
| gh-05 | Peganum harmala L: Tong fiber crops: Sophora alopecuroide: castor bean=20g:50g:30g:40g |
| gh-06 | Peganum harmala L: Tong fiber crops: Sophora alopecuroide: castor bean=50g:50g:50g:50g |
Continued 1
Table 2 is respectively organized raw material soaking condition
Table 3 is respectively organized extraction conditions
| Numbering | Temperature (℃) | Extraction time (min) |
| gh-01 | 20 | 30 |
| gh-02 | 25 | 35 |
| gh-03 | 29 | 40 |
| gh-04 | 32 | 45 |
| gh-05 | 35 | 50 |
Continued 3
| gh-06 | 38 | 55 |
| gh-07 | 40 | 60 |
| gh-08 | 30 | 45 |
| gh-09 | 30 | 45 |
| gh-10 | 30 | 45 |
The preparation of embodiment 2 root knot nematode control agent of the present invention
1, gh-01 is concentrated into 10% of extract, obtains gh-01 concentrate, get the gh-01 concentrate of 5g, add 0.04g fatty alcohol-polyoxyethylene ether, 0.02g organosilicon synergist, stir, dissolve fully, obtain GH-01 aqua.
2, gh-02 is concentrated into 5% of extract, obtains gh-02 concentrate, get the gh-02 concentrate of 5g, add 0.04g fatty alcohol-polyoxyethylene ether, 0.02g organosilicon synergist, stir, dissolve fully, obtain GH-02 aqua.
3, gh-03 is concentrated into 15% of extract, obtains gh-03 concentrate, get the gh-03 concentrate of 5g, add 0.04g fatty alcohol-polyoxyethylene ether, 0.02g organosilicon synergist, stir, dissolve fully, obtain GH-03 aqua.
4, gh-04 is concentrated into 18% of extract, obtains gh-04 concentrate, get the gh-04 concentrate of 5g, add 0.04g fatty alcohol-polyoxyethylene ether, 0.02g organosilicon synergist, stir, dissolve fully, obtain GH-04 aqua.
5, gh-05 is concentrated into 20% of extract, obtains gh-05 concentrate, get the gh-05 concentrate of 10g, add 0.02g organosilicon synergist, 0.04g sodium lignin sulfonate, 2g ammonium sulfate, the potassium dihydrogen phosphate of 10g, stir, be dried, pulverize, obtain GH-05 pulvis.
6, gh-06 is concentrated into 15% of extract, obtains gh-06 concentrate, get 10 gh-06 concentrate, add 0.02g organosilicon synergist, 0.04g sodium lignin sulfonate, 2g ammonium sulfate, the potassium dihydrogen phosphate of 10g, stir, be dried, pulverize, obtain GH-06 pulvis.
7, gh-07 is concentrated into 15% of extract, obtains gh-07 concentrate, get 10 gh-07 concentrate, add 0.02g organosilicon synergist, 0.04g sodium lignin sulfonate, 2g ammonium sulfate, the potassium dihydrogen phosphate of 10g, granulation, dry, mistake 1um sieve, obtain GH-07 granule.
8, gh-08 is concentrated into 15% of extract, obtains gh-08 concentrate, get 10 gh-08 concentrate, add 0.02g organosilicon synergist, 0.04g sodium lignin sulfonate, 2g ammonium sulfate, the potassium dihydrogen phosphate of 10g, granulation, dry, mistake 4.75um sieve, obtain GH-08 granule.
9, gh-09 is concentrated into 15% of extract, obtains gh-07 concentrate, get 10 gh-07 concentrate, add 0.02g organosilicon synergist, 0.04g sodium lignin sulfonate, 2g ammonium sulfate, the potassium dihydrogen phosphate of 10g, granulation, dry, mistake 1um sieve, obtain GH-09 granule.
10, gh-10 is concentrated into 15% of extract, obtains gh-08 concentrate, get 10 gh-08 concentrate, add 0.02g organosilicon synergist, 0.04g sodium lignin sulfonate, 2g ammonium sulfate, the potassium dihydrogen phosphate of 10g, granulation, dry, mistake 4.75um sieve, obtain GH-10 granule.
The experiment of embodiment 3 root knot nematode control agent of the present invention water spinach field efficacy
1, experiment material
For studying thing: water spinach;
Reagent agent: GH-01;
Contrast medicament: 0.5% Avermectin particle Fushan City, Guangdong Province Ying Hui crop science Co., Ltd
2, experimental technique
2.1 reagent agent experimental scheme
Chemicals treatment, amount of application, time of application etc. are in Table 4.
Table 4 reagent agent experimental scheme
Test site: Chongli town, Dongpo District, Meishan city
Weather condition: duration of test temperature 19-31 ℃.Maintain an equal level in precipitation and former years therebetween, without special hazard weather.
Other factors: consumption is consistent as far as possible in each region while being watered solution in pouring, other fertilizer, water, disease, worm, crop smothering management are consistent.
2.2 Quarter Design
Medicament of this test, three concentration, 1 contrast medicament, 1 blank, repeats Gong15Ge community, each community 30m for three times
2.Minizone and surrounding all leave isolation strip, and randomized arrangement is respectively processed in community, and permutation table sees the following form.
Table 5 community permutation table
2.3 investigation methods, content
2.3.1 strain morbidity survey
The method of investigation and sampling has multiple, according to actual conditions, and Yin Qiegen percentage method: do not extract plant, be easy to practical operation, therefore this test adopts this method.During pilot survey, with scuppit, dig 1/2nd root, range estimation root nodule length sum accounts for the percentage of total root length.Concrete grade scale is: 0 grade, without visible root nodule or pieces of an egg; 1 grade, root nodule total length accounts for 1%-24% of total root length; 2 grades, root nodule total length accounts for 25%-49% of total root length; 3 grades, root nodule total length accounts for 50%-74% of total root length; 4 grades, root nodule total length accounts for 75%-100% of total root length.Adopt accidental sampling ，Mei community random searching 5 points, every 2 strain water spinaches, prize root with instrument, observe root root-knot nematode incidence, statistics morbidity amount; Calculate root knot exponential sum control efficiency.
2.3.2 conventional record
On the impact of growth and root knot morbidity classification situation.
2.3.3 control time and number of times
Crop growth period carries out 2 investigation, is respectively after medication for the second time after 7 days and medication for the third time 15 days.
2.3.4 drug effect computational methods
2.3.5 the direct impact on crop
Observe medicament crop is had or not to poisoning, record type and the degree of poisoning.In addition, also to record other beneficial effects (as promoted maturation, stimulating growth etc.) to crop.
By following manner, record poisoning:
If a) poisoning can be measured or be calculated, represent with absolute figure, as plant height.
B) in other cases, can estimate by following two kinds of methods degree and the frequency of poisoning;
1) according to poisoning pulvis method, record the poisoning situation of every community, with following stage division, represent.
Poisoning stage division:
0 grade: without poisoning;
1 grade: slight poisoning, does not affect crop normal growth;
2 grades: moderate poisoning, can restore, can not cause crop failure;
3 grades: severe poisoning, affect normal growth, crop yield and quality are caused to loss to a certain degree;
4 grades: serious poisoning, plant growth is obstructed, and output and mass loss are serious.
2), by chemicals treatment district and the comparison of blank district, evaluate its poisoning percentage.
Meanwhile, the symptom of chemical damage (dwarfing, chlorisis, deformity etc.) of accurate description crop.
2.3.6 the impact on other biological
Record medicament to the impact on other biological in test block.
2.3.7 control time, number of times
The plot that this test is selected is the ground that root-knot nematode occurs, and investigation has carried out twice, is respectively after 10 days and medication for the third time, within 15 days, to investigate incidence after dispenser for the second time.
3 test results and analysis
3.1 result of the test
This test has been carried out 2 investigation ，Mei communities and has been got 5 points, adopts Qie Genfa investigation, and investigation initial data is in Table 5, and test is processed the data obtained by statistics, and its disease index and preventive effect, in Table 6.
Table 6 test data table
Continued 6
The preventive effect of table 7 medicament to root-knot nematode
3.2 interpretation of result
As can be seen from the test results, tri-concentration of GH-01 are processed water spinach and soil, control root-knot nematode is all had to certain preventive effect, after medication for the second time, after 10 days and medication for the third time, investigation in 15 days is all high than the preventive effect of contrast medicament 0.5% Abamectin, the better effects if of wherein processing with B and C, but the preventive effect difference of two consumptions is not remarkable, from economic angle, consider, by 4 kgs/acre uses, can prevent and treat preferably root-knot nematode.
4 conclusion (of pressure testing)s
In sum, this test with medicament GH-01 of institute has certain control efficiency to the root-knot nematode of control water spinach, and drug effect is higher than Avermectin, and consumption can reach desirable control efficiency by 4 kgs/acre uses.
Effect experiment between embodiment 4 root knot nematode control agent of the present invention corn field
1, experiment material
For studying thing: corn;
Reagent agent: GH-02;
Contrast medicament: 0.5% Avermectin particle Fushan City, Guangdong Province Ying Hui crop science Co., Ltd
2, experimental technique
2.1 reagent agent experimental scheme
Chemicals treatment, amount of application, time of application etc. are in Table 8.
Table 8 reagent agent experimental scheme
Test site: 5 groups, southwest township, Pujiang County, Sichuan Province, this ground cutworm, mole cricket are caused harm seriously.
Weather condition: duration of test temperature 14-30 ℃.Maintain an equal level in precipitation and former years therebetween, without special hazard weather.
Other factors: consumption is consistent as far as possible in each region while being watered solution in pouring, other fertilizer, water, disease, worm, crop smothering management are consistent.
2.2 Quarter Design
Medicament of this test, three concentration, 1 contrast medicament, 1 blank, repeats Gong15Ge community, each community 30m for three times
2.Minizone and surrounding all leave isolation strip, and randomized arrangement is respectively processed in community, and arranging situation sees the following form.
Table 9 community permutation table
2.3 investigation methods, content
2.3.1 strain morbidity survey
What this test was selected is experimental field cutworm, the mole cricket serious place of causing harm, and investigation is mainly growing way, the cutworm of maize seedling, the survival volume of mole cricket and is the Harm of corn.
2.3.2 conventional record
On the impact of corn growth and the situation that affects on maize seedling, and the survival volume of cutworm, mole cricket is investigated and record.
2.3.3 control time and number of times
Crop growth period carries out 2 investigation, is respectively after medication for the first time after 7 days and medication for the second time 7 days.
(ask that inventor examine corn control time)
2.3.4 drug effect computational methods
2.3.5 the direct impact on crop
Observe medicament crop is had or not to poisoning, record type and the degree of poisoning.In addition, also to record other beneficial effects (as promoted maturation, stimulating growth etc.) to crop.
By following manner, record poisoning:
If a) poisoning can be measured or be calculated, represent with absolute figure, as plant height.
B) in other cases, can estimate by following two kinds of methods degree and the frequency of poisoning;
1) according to poisoning pulvis method, record the poisoning situation of every community, with following stage division, represent.
Poisoning stage division:
0 grade: without poisoning;
1 grade: slight poisoning, does not affect crop normal growth;
2 grades: moderate poisoning, can restore, can not cause crop failure;
3 grades: severe poisoning, affect normal growth, crop yield and quality are caused to loss to a certain degree;
4 grades: serious poisoning, plant growth is obstructed, and output and mass loss are serious.
2), by chemicals treatment district and the comparison of blank district, evaluate its poisoning percentage.
Meanwhile, the symptom of chemical damage (dwarfing, chlorisis, deformity etc.) of accurate description crop.
2.3.6 the impact on other biological
Record medicament to the impact on other biological in test block.
2.3.7 control time, number of times
The plot that this test is selected is the ground that root-knot nematode occurs, and investigation has carried out twice, is respectively after medication for the first time after 7 days and medication for the second time 7 days.
3 test results and analysis
3.1 result of the test
This test has been carried out 2 investigation ，Mei communities and has been got 5 points, adopts Qie Genfa investigation, and test is processed the data obtained by statistics, and its disease index and preventive effect, in Table 10.
The preventive effect of table 10 medicament to root-knot nematode
3.2 interpretation of result
As can be seen from the test results, tri-concentration of GH-02 are processed corn and soil, control root-knot nematode is all had to certain preventive effect, after medication for the first time, after 7 days and medication for the second time, investigation in 7 days is all high than the preventive effect of contrast medicament 0.5% Abamectin, the better effects if of wherein processing with B and C, but the preventive effect difference of two consumptions is not remarkable, from economic angle, consider, by 4 kgs/acre uses, can prevent and treat preferably root-knot nematode.
4 conclusion (of pressure testing)s
In sum, this test with medicament GH-02 of institute has certain control efficiency to the root-knot nematode of control of maize, and drug effect is higher than Avermectin, and consumption can reach desirable control efficiency by 4 kgs/acre uses.
Effect experiment between embodiment 5 root knot nematode control agent of the present invention Peanut Fields
Adopt the method for embodiment 4 to carry out effect experiment between the Peanut Fields of root knot nematode control agent.
1. experimental pharmacy and disposition thereof are in Table 11.
Table 11 reagent agent experimental scheme
2 Quarter Designs
Medicament of this test, three concentration, 1 contrast medicament, 1 blank, repeats Gong15Ge community, each community 30m for three times
2.Minizone and surrounding all leave isolation strip, and randomized arrangement is respectively processed in community, and arranging situation sees the following form.
Biao12 community permutation table
3 experimental results
Adopt the investigation method, poisoning stage division of embodiment 4, data processing method, within 10 days after dispenser for the first time, carry out data survey for the first time, within after dispenser 10 days for the second time, carry out data survey for the second time, preventive effect the results are shown in Table 13.
The preventive effect of table 13 medicament to root-knot nematode
3.1 interpretation of result
As can be seen from the test results, tri-concentration of GH-03 are processed corn and soil, control root-knot nematode is all had to certain preventive effect, after medication for the first time, after 10 days and medication for the second time, investigation in 10 days is all high than the preventive effect of contrast medicament 0.5% Abamectin, the better effects if of wherein processing with B and C, but the preventive effect difference of two consumptions is not remarkable, from economic angle, consider, by 4 kgs/acre uses, can prevent and treat preferably root-knot nematode.
4 conclusion (of pressure testing)s
In sum, this test with medicament GH-03 of institute has certain control efficiency to the root-knot nematode of control peanut, and drug effect is higher than Avermectin, and consumption can reach desirable control efficiency by 4 kgs/acre uses.
The experiment of embodiment 6 root knot nematode control agent of the present invention pea field efficacy
The method of employing embodiment 4 is carried out the pea field efficacy experiment of root knot nematode control agent.
1. experimental pharmacy and disposition thereof are in Table 14.
Table 14 reagent agent experimental scheme
2 Quarter Designs
Medicament of this test, three concentration, 1 contrast medicament, 1 blank, repeats Gong15Ge community, each community 30m for three times
2.Minizone and surrounding all leave isolation strip, and randomized arrangement is respectively processed in community, and arranging situation sees the following form.
Biao15 community permutation table
3 experimental results
Adopt investigation method, poisoning stage division and the data processing method of embodiment 4, within 7 days after dispenser for the first time, carry out data survey for the first time, within after dispenser 7 days for the second time, carry out data survey for the second time, preventive effect the results are shown in Table 16.
The preventive effect of table 16 medicament to root-knot nematode
3.1 interpretation of result
As can be seen from the test results, tri-concentration of GH-04 are processed pea and soil, control root-knot nematode is all had to certain preventive effect, after medication for the first time, after 7 days and medication for the second time, investigation in 7 days is all high than the preventive effect of contrast medicament 0.5% Abamectin, the better effects if of wherein processing with B and C, but the preventive effect difference of two consumptions is not remarkable, from economic angle, consider, by 4 kgs/acre uses, can prevent and treat preferably root-knot nematode.
4 conclusion (of pressure testing)s
In sum, this test with medicament GH-04 of institute has certain control efficiency to the root-knot nematode of control pea, and drug effect is higher than Avermectin, and consumption can reach desirable control efficiency by 4 kgs/acre uses.
The experiment of embodiment 7 root knot nematode control agent of the present invention carrot field efficacy
Adopt the method for embodiment 4 to carry out effect experiment between the Peanut Fields of root knot nematode control agent.
1 experimental pharmacy and disposition thereof are in Table 17.
Table 17 reagent agent experimental scheme
2 Quarter Designs
Medicament of this test, three concentration, 1 contrast medicament, 1 blank, repeats Gong15Ge community, each community 30m for three times
2.Minizone and surrounding all leave isolation strip, and randomized arrangement is respectively processed in community, and arranging situation sees the following form.
Biao18 community permutation table
3 experimental results
Adopt investigation method, poisoning stage division and the data processing method of embodiment 4, within 7 days after dispenser for the first time, carry out data survey for the first time, within after dispenser 7 days for the second time, carry out data survey for the second time, preventive effect the results are shown in Table 19.
The preventive effect of table 19 medicament to root-knot nematode
3.1 interpretation of result
As can be seen from the test results, tri-concentration of GH-05 are processed carrot and soil, control root-knot nematode is all had to certain preventive effect, after medication for the first time, after 7 days and medication for the second time, investigation in 7 days is all high than the preventive effect of contrast medicament 0.5% Abamectin, the better effects if of wherein processing with B and C, but the preventive effect difference of two consumptions is not remarkable, from economic angle, consider, by 4 kgs/acre uses, can prevent and treat preferably root-knot nematode.
4 conclusion (of pressure testing)s
In sum, this test with medicament GH-05 of institute has certain control efficiency to the root-knot nematode of control carrot, and drug effect is higher than Avermectin, and consumption can reach desirable control efficiency by 4 kgs/acre uses.
The experiment of embodiment 8 root knot nematode control agent of the present invention Chinese cabbage field efficacy
The method of employing embodiment 4 is carried out the Chinese cabbage field efficacy experiment of root knot nematode control agent.
1. experimental pharmacy and disposition thereof are in Table 20.
Table 20 reagent agent experimental scheme
Continued 20
2 Quarter Designs
Medicament of this test, three concentration, 1 contrast medicament, 1 blank, repeats Gong15Ge community, each community 30m for three times
2.Minizone and surrounding all leave isolation strip, and randomized arrangement is respectively processed in community.
Biao21 community permutation table
3 experimental results
Adopt investigation method, poisoning stage division and the data processing method of embodiment 4, within 7 days after dispenser for the first time, carry out data survey for the first time, within after dispenser 7 days for the second time, carry out data survey for the second time, preventive effect the results are shown in Table 22.
The preventive effect of table 22 medicament to root-knot nematode
3.1 interpretation of result
As can be seen from the test results, tri-concentration of GH-06 are processed Chinese cabbage and soil, control root-knot nematode is all had to certain preventive effect, after medication for the first time, after 7 days and medication for the second time, investigation in 7 days is all high than the preventive effect of contrast medicament 0.5% Abamectin, the better effects if of wherein processing with B and C, but the preventive effect difference of two consumptions is not remarkable, from economic angle, consider, by 4 kgs/acre uses, can prevent and treat preferably root-knot nematode.
4 conclusion (of pressure testing)s
In sum, this test with medicament GH-06 of institute has certain control efficiency to the root-knot nematode of control Chinese cabbage, and drug effect is higher than Avermectin, and consumption can reach desirable control efficiency by 4 kgs/acre uses.
The experiment of embodiment 9 root knot nematode control agent of the present invention lettuce field efficacy
The method of employing embodiment 4 is carried out the lettuce field efficacy experiment of root knot nematode control agent.
1 experimental pharmacy and disposition thereof are in Table 23.
Table 23 reagent agent experimental scheme
2 Quarter Designs
Medicament of this test, three concentration, 1 contrast medicament, 1 blank, repeats Gong15Ge community, each community 30m for three times
2.Minizone and surrounding all leave isolation strip, and randomized arrangement is respectively processed in community, and arranging situation sees the following form.
Biao24 community permutation table
3 experimental results
Adopt investigation method, poisoning stage division and the data processing method of embodiment 4, within 10 days after dispenser for the first time, carry out data survey for the first time, within after dispenser 10 days for the second time, carry out data survey for the second time, preventive effect the results are shown in Table 25.
The preventive effect of table 25 medicament to root-knot nematode
3.1 interpretation of result
As can be seen from the test results, tri-concentration of GH-07 are processed lettuce and soil, control root-knot nematode is all had to certain preventive effect, after medication for the first time, after 10 days and medication for the second time, investigation in 10 days is all high than the preventive effect of contrast medicament 0.5% Abamectin, the better effects if of wherein processing with B and C, but the preventive effect difference of two consumptions is not remarkable, from economic angle, consider, by 4 kgs/acre uses, can prevent and treat preferably root-knot nematode.
4 conclusion (of pressure testing)s
In sum, this test with medicament GH-07 of institute has certain control efficiency to the root-knot nematode of control peanut, and drug effect is higher than Avermectin, and consumption can reach desirable control efficiency by 4 kgs/acre uses.
Effect experiment between embodiment 10 root knot nematode control agent of the present invention eggplant field
Adopt the method for embodiment 4 to carry out effect experiment between the eggplant field of root knot nematode control agent.
1. experimental pharmacy and disposition thereof are in Table 26.
Table 26 reagent agent experimental scheme
Continued 26
2 Quarter Designs
Medicament of this test, three concentration, 1 contrast medicament, 1 blank, repeats Gong15Ge community, each community 30m for three times
2.Minizone and surrounding all leave isolation strip, and randomized arrangement is respectively processed in community.
Biao27 community permutation table
3 experimental results
Adopt investigation method, poisoning stage division and the data processing method of embodiment 4, within 10 days after dispenser for the first time, carry out data survey for the first time, within after dispenser 10 days for the second time, carry out data survey for the second time, preventive effect the results are shown in Table 28.
The preventive effect of table 28 medicament to root-knot nematode
3.1 interpretation of result
As can be seen from the test results, tri-concentration of GH-08 are processed eggplant and soil, control root-knot nematode is all had to certain preventive effect, after medication for the first time, after 10 days and medication for the second time, investigation in 10 days is all high than the preventive effect of contrast medicament 0.5% Abamectin, the better effects if of wherein processing with B and C, but the preventive effect difference of two consumptions is not remarkable, from economic angle, consider, by 4 kgs/acre uses, can prevent and treat preferably root-knot nematode.
4 conclusion (of pressure testing)s
In sum, this test with medicament GH-08 of institute has certain control efficiency to the root-knot nematode of control eggplant, and drug effect is higher than Avermectin, and consumption can reach desirable control efficiency by 4 kgs/acre uses.
Claims (10)
1. root knot nematode control agent, is characterized in that its active component is prepared from by the raw material of following weight portion: the Tong fiber crops of the ginkgo leaf of the peganum harmala L of 1～8 weight portion, 0～8 weight portion, the Sophora alopecuroide of 1～10 weight portion, the root of subprostrate sophora of 0～5 weight portion, the kuh-seng of the asiatic moonseed of 0～5 weight portion, 0～10 weight portion, the castor bean of 0～8 weight portion, 1～10 weight portion, the garland chrysanthemum of 0～8 weight portion are, the datura of 0～5 weight portion.
2. root knot nematode control agent according to claim 1, is characterized in that its active component is prepared from by the raw material of following weight portion:
The Tong fiber crops of the castor bean of the kuh-seng of the asiatic moonseed of the root of subprostrate sophora of the Sophora alopecuroide of the ginkgo leaf of the peganum harmala L of 1～8 weight portion, 0.5～8 weight portion, 1～10 weight portion, 0～5 weight portion, 0～5 weight portion, 0～10 weight portion, 0～8 weight portion, 1～10 weight portion, the garland chrysanthemum of 3～8 weight portions are, the datura of 1～5 weight portion;
Or the peganum harmala L of 1～5 weight portion, the Sophora alopecuroide of 1～6 weight portion are, the Tong of 1～8 weight portion is numb, the ginkgo leaf of 1～7 weight portion is, the castor bean of the kuh-seng of 1～5 weight portion, 1～8 weight portion;
Or the peganum harmala L of 1～5 weight portion, the Sophora alopecuroide of 1～5 weight portion, the castor bean of the piemarker of 1～5 weight portion, 0.5～6 weight portion and the ginkgo leaf of 1～6 weight portion;
Or the Tong of the peganum harmala L of 2～5 weight portions, 2～5 weight portions fiber crops, the Sophora alopecuroide of 2～5 weight portions and the castor bean of 2～5 weight portions.
3. root knot nematode control agent according to claim 1 and 2, is characterized in that: its active component is got and is prepared from through ethanolic solution or water extraction by each raw material.
4. according to the root knot nematode control agent described in claim 1～3 any one, it is characterized in that: described root knot nematode control agent is aqua, pulvis or granule.
5. root knot nematode control agent according to claim 4, is characterized in that: in described pulvis and granule, also comprise carrier, described carrier is maltose, soluble starch or fertilizer element; Wherein, described fertilizer element is at least one in potassium sulphate, ammonium sulfate, MAP, diammonium phosphate, magnesium sulfate, potassium dihydrogen phosphate or organic matter; Organic is agricultural crop straw at least one that thing, careless carbon, ight soil become thoroughly decomposed in thing of becoming thoroughly decomposed.
6. the preparation method of the root knot nematode control agent described in claim 1～5 any one, is characterized in that comprising the steps:
A, pulverizing: raw material is mixed and pulverize;
B, immersion: the raw material after the pulverizing of a step is added in solvent and soaks to obtain immersion mixture, and soak time is 12～36 hours, preferably 18～30 hours; Wherein, described solvent is ethanol water, and the mass ratio of described raw material and solvent is 1:20～50, preferred 1:30～40, and in solvent, the mass ratio of water and ethanol is 0.25～4:1, preferably 0.5～1:1;
C, extraction: by b step gained immersion mixture ultrasonic lixiviate 30～60min under 20～40 ℃ of conditions, obtain extract;
D, preparation: extract is made to aqua, pulvis or granule.
7. the preparation method of the root knot nematode control agent described in claim 1～5 any one, is characterized in that comprising the steps:
A, pulverizing: raw material is mixed and pulverize;
B, preparation: the raw material after a step is pulverized is made pulvis.
8. the preparation method of the root knot nematode control agent described in claim 1～5 any one, is characterized in that comprising the steps:
A, pulverizing: raw material is mixed and pulverize;
B, fermentation maturity: the fermenting raw materials after a is pulverized or become thoroughly decomposed;
C, preparation: the raw material after becoming thoroughly decomposed is by fermentation made to pulvis or granule.
9. the application of the root knot nematode control agent described in claim 1～5 any one in control plant root-knot nematode.
10. the application of root knot nematode control agent according to claim 9 in control plant root-knot nematode, it is characterized in that: described root knot nematode control agent, for being subject to the plant of root-knot nematode evil, is preferably water spinach, cucumber, tomato, carrot, Chinese cabbage, lettuce, eggplant, custard squash; More preferably will aqua pour into a mould, spray on plant leaf surface after dilution, or pulvis and granule are dissolved in the water, after dilution, pour into a mould, spray on plant leaf surface, or by pulvis, granule dusting, intersperse among in plant soil, rewater.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310601194.6A CN103583606B (en) | 2013-11-21 | 2013-11-21 | Root knot nematode control agent and its preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310601194.6A CN103583606B (en) | 2013-11-21 | 2013-11-21 | Root knot nematode control agent and its preparation method and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103583606A true CN103583606A (en) | 2014-02-19 |
| CN103583606B CN103583606B (en) | 2016-02-17 |
Family
ID=50074095
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310601194.6A Active CN103583606B (en) | 2013-11-21 | 2013-11-21 | Root knot nematode control agent and its preparation method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103583606B (en) |
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104430557A (en) * | 2014-11-15 | 2015-03-25 | 辽宁农业职业技术学院 | Chinese herbal medicine composition for preventing and treating plant root-knot nematode as well as preparation method and application thereof |
| CN105211129A (en) * | 2015-09-17 | 2016-01-06 | 张超 | A kind of composition pesticide of deinsectization ovum |
| CN105284937A (en) * | 2015-11-30 | 2016-02-03 | 南京中医药大学 | Traditional Chinese medicine extract for resisting Chinese angelica coarse barkdiseases and application of traditional Chinese medicine extract |
| CN105330461A (en) * | 2015-11-23 | 2016-02-17 | 杨*** | Biological medicine fertilizer for controlling plant root knot nematodes as well as preparation method and application of biological medicine fertilizer |
| CN105646011A (en) * | 2016-01-11 | 2016-06-08 | 济南康众医药科技开发有限公司 | New application of trogopterus dung |
| CN105875293A (en) * | 2016-06-07 | 2016-08-24 | 青川县宏宇猕猴桃种植专业合作社 | Kiwi fruit canker prevention method and prevention agent |
| CN105993493A (en) * | 2016-05-29 | 2016-10-12 | 寿光市新世纪种苗有限公司 | Biological trapping method for controlling colored bell pepper root-knot nematode |
| CN106577796A (en) * | 2016-11-22 | 2017-04-26 | 山东省农业科学院蔬菜花卉研究所 | Method of prevention and treatment of tomato root knot nematode disease by castor bean meal |
| CN106614814A (en) * | 2016-08-02 | 2017-05-10 | 安树义 | Production process of veratrine nematicide |
| CN106889116A (en) * | 2017-02-27 | 2017-06-27 | 青岛海之星生物科技有限公司 | A kind of peanut root-knot nematode attractant and preparation method thereof |
| CN107512986A (en) * | 2017-10-03 | 2017-12-26 | 长沙仲善新能源科技有限公司 | Synergy environment-friendlyfertilizer fertilizer of improvement plantation forest soil and preparation method thereof |
| CN109452307A (en) * | 2018-11-27 | 2019-03-12 | 成都新朝阳作物科学有限公司 | The water-soluble biological organic fertilizer and preparation method thereof for preventing and treating root-knot nematode |
| CN109650997A (en) * | 2019-01-30 | 2019-04-19 | 沈阳禾康永丰生物环保产业有限公司 | A kind of plant root-knot nematode administering method |
| CN111567571A (en) * | 2020-05-15 | 2020-08-25 | 湖北大学 | Plant source compound meloidogyne incognita insecticide and preparation method thereof |
| CN113994960A (en) * | 2021-12-01 | 2022-02-01 | 江西省农业科学院植物保护研究所 | Pharmaceutical composition and pharmaceutical preparation for preventing and treating peanut root-knot nematode and preparation method thereof |
| CN114158508A (en) * | 2021-11-30 | 2022-03-11 | 海南归耘田农业科技有限公司 | Method for effectively preventing crayfish from being infected by parasite |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009093079A2 (en) * | 2008-01-23 | 2009-07-30 | Arab Science And Technology Foundation | Environmentally friendly plant protection agents |
| CN102775238A (en) * | 2012-08-22 | 2012-11-14 | 乌鲁木齐木易联华生物科技有限公司 | Organic plant pesticide fertilizer with pesticidal and bactericidal effects and production technique thereof |
-
2013
- 2013-11-21 CN CN201310601194.6A patent/CN103583606B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009093079A2 (en) * | 2008-01-23 | 2009-07-30 | Arab Science And Technology Foundation | Environmentally friendly plant protection agents |
| CN102775238A (en) * | 2012-08-22 | 2012-11-14 | 乌鲁木齐木易联华生物科技有限公司 | Organic plant pesticide fertilizer with pesticidal and bactericidal effects and production technique thereof |
Non-Patent Citations (6)
| Title |
|---|
| 刘计权 等: "植物源农药防治根结线虫研究进展", 《农药》, vol. 50, no. 6, 30 June 2011 (2011-06-30), pages 395 - 398 * |
| 徐晓莉: "茶籽粗提物杀线虫活性初步研究", 《中国优秀硕士学位论文全文数据库农业科技辑》, no. 10, 15 October 2008 (2008-10-15) * |
| 杜小凤 等: "苦豆子甲醇提取物对南方根结线虫的生物活性", 《江西农业学报》, vol. 22, no. 11, 31 December 2010 (2010-12-31), pages 88 - 90 * |
| 谢宁: "苘麻提取物对南方根结线虫生物活性测定及活性成份的分离", 《山东农业大学硕士学位论文》, 6 April 2013 (2013-04-06) * |
| 邱雪柏: "杀线虫植物的筛选及对根结线虫防治作用的研究", 《中国优秀硕士学位论文全文数据库农业科技辑》, no. 1, 31 December 2011 (2011-12-31) * |
| 闫磊 等: "不同植物提取物对马铃薯茎线虫的活性筛选", 《山东农业大学学报(自然科学版)》, vol. 39, no. 2, 31 December 2008 (2008-12-31), pages 223 - 228 * |
Cited By (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104430557A (en) * | 2014-11-15 | 2015-03-25 | 辽宁农业职业技术学院 | Chinese herbal medicine composition for preventing and treating plant root-knot nematode as well as preparation method and application thereof |
| CN105211129A (en) * | 2015-09-17 | 2016-01-06 | 张超 | A kind of composition pesticide of deinsectization ovum |
| CN105330461A (en) * | 2015-11-23 | 2016-02-17 | 杨*** | Biological medicine fertilizer for controlling plant root knot nematodes as well as preparation method and application of biological medicine fertilizer |
| CN105284937A (en) * | 2015-11-30 | 2016-02-03 | 南京中医药大学 | Traditional Chinese medicine extract for resisting Chinese angelica coarse barkdiseases and application of traditional Chinese medicine extract |
| CN105284937B (en) * | 2015-11-30 | 2018-03-09 | 南京中医药大学 | A kind of Chinese medical extract of anti-Radix Angelicae Sinensis fiber crops stomatosis and its application |
| CN105646011A (en) * | 2016-01-11 | 2016-06-08 | 济南康众医药科技开发有限公司 | New application of trogopterus dung |
| CN105993493A (en) * | 2016-05-29 | 2016-10-12 | 寿光市新世纪种苗有限公司 | Biological trapping method for controlling colored bell pepper root-knot nematode |
| CN105875293A (en) * | 2016-06-07 | 2016-08-24 | 青川县宏宇猕猴桃种植专业合作社 | Kiwi fruit canker prevention method and prevention agent |
| CN106614814A (en) * | 2016-08-02 | 2017-05-10 | 安树义 | Production process of veratrine nematicide |
| CN106577796A (en) * | 2016-11-22 | 2017-04-26 | 山东省农业科学院蔬菜花卉研究所 | Method of prevention and treatment of tomato root knot nematode disease by castor bean meal |
| CN106889116A (en) * | 2017-02-27 | 2017-06-27 | 青岛海之星生物科技有限公司 | A kind of peanut root-knot nematode attractant and preparation method thereof |
| CN107512986A (en) * | 2017-10-03 | 2017-12-26 | 长沙仲善新能源科技有限公司 | Synergy environment-friendlyfertilizer fertilizer of improvement plantation forest soil and preparation method thereof |
| CN109452307A (en) * | 2018-11-27 | 2019-03-12 | 成都新朝阳作物科学有限公司 | The water-soluble biological organic fertilizer and preparation method thereof for preventing and treating root-knot nematode |
| CN109452307B (en) * | 2018-11-27 | 2021-10-08 | 成都新朝阳作物科学股份有限公司 | Water-soluble biological organic fertilizer for preventing and treating root-knot nematode and preparation method thereof |
| CN109650997A (en) * | 2019-01-30 | 2019-04-19 | 沈阳禾康永丰生物环保产业有限公司 | A kind of plant root-knot nematode administering method |
| CN111567571A (en) * | 2020-05-15 | 2020-08-25 | 湖北大学 | Plant source compound meloidogyne incognita insecticide and preparation method thereof |
| CN111567571B (en) * | 2020-05-15 | 2021-09-03 | 湖北大学 | Botanical compound insecticide for meloidogyne incognita and preparation method thereof |
| CN114158508A (en) * | 2021-11-30 | 2022-03-11 | 海南归耘田农业科技有限公司 | Method for effectively preventing crayfish from being infected by parasite |
| CN113994960A (en) * | 2021-12-01 | 2022-02-01 | 江西省农业科学院植物保护研究所 | Pharmaceutical composition and pharmaceutical preparation for preventing and treating peanut root-knot nematode and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103583606B (en) | 2016-02-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103583606B (en) | Root knot nematode control agent and its preparation method and application | |
| CN103864510B (en) | One kind sustained release composite fertilizer with pesticide | |
| CN105612986B (en) | The implantation methods of selenium-rich dragon fruit | |
| CN103864511B (en) | A kind of synergy composite organic-inorganic fertilizer | |
| CN103688813B (en) | A kind of implantation methods reducing rice absorbing heavy metal cadmium | |
| CN104770368B (en) | Bactericidal composition and its application containing SYP-3375 and octyl trichlamide | |
| CN107628886A (en) | A kind of special micro-balance Eco-fertilizer of yellow peach and preparation method thereof | |
| KR20110033691A (en) | Environment-friendly organic agricultural products for soil insect pest control | |
| CN106900305A (en) | A kind of implantation methods of selenium-rich herbaceous coffee | |
| CN106365729A (en) | Soil remediation organic fertilizer for overcoming panax notoginseng continuous cropping obstruction, preparation method and application of soil remediation organic fertilizer | |
| CN108450273A (en) | The method that salt-soda soil plants rose and improves clay fertilizer situation | |
| CN102960160A (en) | Planting method for producing aloe agricultural products | |
| CN105874956A (en) | A method of directly returning maize straw to fields by adding a decomposition agent | |
| CN104285772A (en) | Cultivating substrate and preparation method thereof and ginseng planting method | |
| CN106212154A (en) | Karst Rocky Desertification mountain region macadimia nut cultural method | |
| CN104996228A (en) | Comprehensive control method for tomato prodenia litura | |
| CN102633566B (en) | Pesticide fertilizer | |
| CN111789009A (en) | Fertilizing method for relieving bletilla striata continuous cropping obstacles | |
| CN103772033A (en) | Biological fertilizer made of radix euphorbiae lantu | |
| CN106118675A (en) | Biogas residue sour soil modifying agent and using method thereof | |
| CN106172425A (en) | A kind of containing octyl trichlamide and the bactericidal composition of propiconazole and application | |
| CN109265286A (en) | A kind of bio-stimulant and its preparation method and application for improving soil phophorus and utilizing | |
| CN104609986A (en) | Fertilizer for controlling red roots and red fruits of dwarf lilyturf tuber | |
| CN107771623A (en) | A kind of high-yield planting method of bamboo shoots | |
| CN102972174A (en) | Planting method for producing ginseng agricultural products |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP01 | Change in the name or title of a patent holder | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 611630 No. five, No. 35 Industrial Road, Heshan Town, Pujiang County, Sichuan, Chengdu Patentee after: Chengdu new Chaoyang Crop Science Co., Ltd Address before: 611630 No. five, No. 35 Industrial Road, Heshan Town, Pujiang County, Sichuan, Chengdu Patentee before: CHENGDU NEWSUN CROPSCIENCE Co.,Ltd. |