CN103540587B - 靶向整合外源DNA序列到大鼠和小鼠Rosa26位点的方法及其应用 - Google Patents
靶向整合外源DNA序列到大鼠和小鼠Rosa26位点的方法及其应用 Download PDFInfo
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- CN103540587B CN103540587B CN201310455151.1A CN201310455151A CN103540587B CN 103540587 B CN103540587 B CN 103540587B CN 201310455151 A CN201310455151 A CN 201310455151A CN 103540587 B CN103540587 B CN 103540587B
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Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN103923940B (zh) * | 2014-04-16 | 2016-08-17 | 中国农业大学 | 一种定点整合外源基因的方法 |
CN109943593B (zh) * | 2019-03-28 | 2021-12-24 | 上海市中医老年医学研究所 | Mir3061基因Rosa26定点敲入杂合子小鼠模型构建方法与应用 |
CN111718932A (zh) * | 2020-06-08 | 2020-09-29 | 中国人民解放军军事科学院军事医学研究院 | 一种新型的基因编辑动物生物反应器制备方法及应用 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN102628037A (zh) * | 2012-03-31 | 2012-08-08 | 西南大学 | 家蚕油蚕基因BmBlos2遗传改造***及其制备方法和应用 |
CN103146735A (zh) * | 2012-12-28 | 2013-06-12 | 西北农林科技大学 | Tale重复单元四聚体库的构建方法、talen表达载体的构建方法及其应用 |
CN103266122A (zh) * | 2013-05-31 | 2013-08-28 | 云南农业大学 | 一种利用talen技术的高效定点转基因方法 |
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Publication number | Priority date | Publication date | Assignee | Title |
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CN102628037A (zh) * | 2012-03-31 | 2012-08-08 | 西南大学 | 家蚕油蚕基因BmBlos2遗传改造***及其制备方法和应用 |
CN103146735A (zh) * | 2012-12-28 | 2013-06-12 | 西北农林科技大学 | Tale重复单元四聚体库的构建方法、talen表达载体的构建方法及其应用 |
CN103266122A (zh) * | 2013-05-31 | 2013-08-28 | 云南农业大学 | 一种利用talen技术的高效定点转基因方法 |
Non-Patent Citations (4)
Title |
---|
Genetic engineering of human pluripotent cells using TALE nucleases;Dirk Hockemeyer et al.;《Nature Biotechnology》;20110707;第731-734页 * |
TALEN构建与斑马鱼基因组定点突变的试验方法与流程;沈延 等;《遗传》;20130430;第533-544页 * |
张金脉等.TALENs:一种新的基因定点修饰技术.《生命科学》.2013,第126-132页. * |
类转录激活因子效应物核酸酶(TALEN)介导的基因组定点修饰技术;沈延 等;《遗传》;20130430;第395-409页 * |
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