CN103461254A - Scale artificial propagation method for procypris rabaudi - Google Patents

Scale artificial propagation method for procypris rabaudi Download PDF

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Publication number
CN103461254A
CN103461254A CN2013104499122A CN201310449912A CN103461254A CN 103461254 A CN103461254 A CN 103461254A CN 2013104499122 A CN2013104499122 A CN 2013104499122A CN 201310449912 A CN201310449912 A CN 201310449912A CN 103461254 A CN103461254 A CN 103461254A
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pin
raun
body weight
parturition
insemination
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周剑
赵刚
杜军
马世荣
周波
陈彦伶
肖宇
何斌
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FISHERIES INSTITUTE SICHUAN ACADEMY OF AGRICULTURAL SCIENCES
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FISHERIES INSTITUTE SICHUAN ACADEMY OF AGRICULTURAL SCIENCES
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Publication of CN103461254A publication Critical patent/CN103461254A/en
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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish

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Abstract

The invention discloses a scale artificial propagation method for procypris rabaudi. The method comprises the steps: (1) choice of parents: 3-5 years old of parent fish, which are strong in physique and free from diseases and injury, are selected; (2) spawning induction: a two-needle or three-needle injection method is adopted to perform spawning induction; (3) artificial insemination; (4) incubation with slow flowing water: the incubation water temperature is 18-23 DEG C, and 5ppm of potassium permanganate is utilized to soak eggs for at least 1 minute every day in the incubation process; (5) nursing during an alevin stage: after fries freely swim and open mouths, the fries are cultivated for a short term in a small indoor cement pit with slow flowing water, and then the fries are converted into an outdoor cement pit for cultivation after the fries grow to be longer than 1 cm. According to the scale artificial propagation method for the procypris rabaudi, the spawning rate, hatching rate and fry survival rate of the parent fish can be greatly improved, and the large scale artificial propagation can be performed.

Description

The former carp large-scale artificial of rock propagation method
technical field:
The present invention relates to the former carp scale of a kind of rock chemical industry propagation method.
background technology:
The former carp of rock procypris rabaudi(Tchang) be the fish on bottom layer of a kind of perch between ChangJiang River basin rivers rock seam, be subordinate to Cypriniformes, Cyprinidae, carp subfamily, Procypris, be commonly called as rock carp, black carp, black carp.Mainly being distributed in ，Yi Jia Lingjiang River, each tributary of Upper Yangtze River and Ming River more, is secondly one of fluviatic famous and precious economic fish in ，Shi Sichuan, main stream of the Yangtze, and the cultivation prospect is very good.
Waterman author has done much research to the former carp of artificial propagation rock in recent years, the former carp artificial propagation techniques of existing rock is raised and train parent population, parent fish rearing and selection, hasten parturition and more detailed research has been done in the aspect such as hatching, provides a kind of easier former carp of rock extensive method of cultivating.But there are the shortcomings such as the parent population spawning rate is not high, dose of medicament is bigger than normal, and cost is higher, and the hatching survival rate is low in the method, is difficult to adapt to the requirement of extensive artificial propagation.
summary of the invention:
The present invention is directed to the shortcoming of the existing former carp artificial propagation techniques of rock, the objective of the invention is for a kind of Gunther parent fish spawning rate, incubation rate, the weed survival rate height former carp large-scale artificial of the rock propagation method of artificial propagation on a large scale is provided.
The inventive method has improved Gunther parent fish spawning rate, incubation rate, weed survival rate greatly.
The object of the present invention is achieved like this:
The former carp large-scale artificial of rock of the present invention propagation method, the method comprises the following steps:
(1) parent's selection: with the physique stalwartness, anosis without the wound, 3～5 age sexually matured parent population;
(2) hasten parturition: take two pins or three pin injections to hasten parturition, the good raun for maturity, water temperature can be hastened parturition in the time of 18～21 ℃, and oxytocic drug and consumption are: the PG(fish hypophysis) every kg body weight is 8～10mg, LRH-A 2(luteotropin releasing hormone d-ala analog) every kg body weight is 3～6ug; The HCG(human chorionic gonadtropin) every kg body weight is 1200～1800IU, adopts two pin injections: the first pin injection is injected remaining all dosage for the 1/8～1/6, the second pin of accumulated dose; The bad parent population for maturity, adopt three pins injections: the first pin be the pin that accelerates the ripening, and raun is injected LRH-A 21ug/kg, through 15～20 d, be checked through raun abdominal distention and soft, can be hastened parturition, second and third pin is the pin of hastening parturition, and the water temperature of hastening parturition is in the time of 20～24 ℃, the needle gage of second and third pin is 8～12 h, and the oxytocic drug of second and third pin and consumption are: the every kg body weight of PG is 6～10mg; LRH-A 2every kg body weight is 3～5ug, and the every kg body weight of HCG is 1000～1600IU, and the second pin injection is injected remaining all dosage for the 1/4～1/3, three pin of accumulated dose, and milter all adopts disposable injection, and dosage is 1/2 of raun accumulated dose;
(3) artificial insemination: hasten parturition after 12 h, parent population has been caught, checked: if raun can extrude the ovum grain, carry out artificial insemination immediately, if raun can not extrude the ovum grain, again parent population be checked every 2h, take the dry method insemination;
(4) miniflow water hatching, the hatching water temperature is at 18-23 ℃, in hatching process, apply 5ppm potassium permanganate soak 0.8～1.2min every day, and select in time bad ovum, the ovum of not inseminating, dead ovum;
(5) nursing of alevin stage: after fry is freely put down trip and opening, carry out short cultivation in indoor miniflow water cement pond, yolk, the wheel animalcule after 80 order screen cloths filter of throwing something and feeding every day 2-4 times, its survival rate can reach more than 90%, grow to 1cm when above, forward outdoor tank-culture to and cultivate, and the cladocera of throwing something and feeding, copepoda, fry grows to 2cm when above, and the water earthworm starts to throw something and feed.
Above-mentioned dry method insemination is with dry towel, female, milter belly to be dried, first gently press the raun belly, fish-egg is bonded on attachment, rapidly the milter seminal fluid is directly clamp-oned again, by fish-egg and the seminal fluid about 1min of uniform stirring gently, add Spawning water standing, wash away seminal fluid, during insemination, should avoid the sunlight direct irradiation.
Through a large amount of tests with repeatedly over a long time, the inventive method improves Gunther parent fish spawning rate 40% than commonsense method, improves incubation rate 50%, improves weed survival rate 50%, and spawning rate, incubation rate, weed survival rate all maintain the leading position in the same industry.This method is the former carp fry of amount reproduction rock on a large scale, and guarantees the original seed of its germplasm, can also reduce disease, improves the survival rate of the former carp of rock.
embodiment:
The former carp large-scale artificial of the present embodiment rock propagation method comprises the following steps:
(1) parent's selection: select the physique stalwartness, anosis without wound, 3～5 age sexually matured parent population: the belly of raun expands softness, and the ovary profile is obvious, and gonopore swelling is slightly micro-red; Milter has star-pursuing in blocks at the rhynchodaenm front end, and fingerprint has coarse sensation, with have gentle hands, presses belly to have seminal fluid to flow out, and after water breakthrough, scatters immediately, is creamy white.
(2) hasten parturition: take two pins or three pin injections to hasten parturition, raun, the water temperature good for maturity can be hastened parturition in the time of 18～21 ℃, and oxytocic drug and consumption are: the every kg body weight of PG is 8～10mg, LRH-A 2every kg body weight is 4.5ug, and the every kg body weight of HCG is 1500IU, mixes use, adopts two pin injections: the first pin injection is injected remaining all dosage for 1/7, the second pin of accumulated dose; The bad parent population for maturity, adopt three pins injections: the first pin be the pin that accelerates the ripening, and raun is injected LRH-A 21ug/kg, through 15～20 d, be checked through raun abdominal distention and soft, can be hastened parturition, second and third pin is the pin of hastening parturition, and the water temperature of hastening parturition is when 22 ℃ of left and right, the needle gage of second and third pin is 10h, and the oxytocic drug of second and third pin and consumption are: the every kg body weight of PG is 8mg, LRH-A 2every kg body weight is 4ug, and the every kg body weight of HCG is 1300IU, and the second pin injection is injected remaining all dosage for 1/4, the three pin of accumulated dose, and milter all adopts disposable injection, and dosage is 1/2 of raun accumulated dose.
(3) artificial insemination: hasten parturition after 12 h, parent population has been caught, checked.If raun can be extruded the ovum grain, be carried out artificial insemination immediately, if raun can not be extruded the ovum grain, parent population be checked every 2h again, take the dry method insemination, with dry towel, female, milter belly are dried, first the light raun belly of pressing, fish-egg is bonded on attachment, rapidly the milter seminal fluid is directly clamp-oned again, by fish-egg and the seminal fluid about 1min of uniform stirring gently, added Spawning water standing, wash away seminal fluid, during insemination, should avoid the sunlight direct irradiation.
(4) miniflow water hatching, the hatching water temperature is 20 ℃ of left and right, in hatching process, apply 5ppm potassium permanganate soak 1min every day, and select in time bad ovum, the ovum of not inseminating, dead ovum.
(5) nursing of alevin stage: after fry is freely put down trip and opening, carry out short cultivation in indoor miniflow water Small cement pond, yolk, the wheel animalcule after 80 order screen cloths filter of throwing something and feeding every day 3 times, its survival rate can reach more than 90%, grow to 1cm when above, forward outdoor tank-culture to and cultivate until it, and start to throw something and feed cladocera, copepoda, grow to 2cm when above until fry, the water earthworm of throwing something and feeding.
Above-described embodiment is that foregoing of the present invention is further described, but this should be interpreted as to the scope of the above-mentioned theme of the present invention only limits to above-described embodiment.All technology realized based on foregoing all belong to scope of the present invention.

Claims (2)

1. the former carp large-scale artificial of rock propagation method, the method comprises the following steps:
(1) parent's selection: with the physique stalwartness, anosis without the wound, 3～5 age sexually matured parent population;
(2) hasten parturition: take two pins or three pin injections to hasten parturition, the good raun for maturity, water temperature can be hastened parturition in the time of 18～21 ℃, and oxytocic drug and consumption are: the every kg body weight of PG is 8～10mg, LRH-A 2every kg body weight is 3～6ug, and the every kg body weight of HCG is 1200～1800IU, adopts two pin injections: the first pin injection is injected remaining all dosage for the 1/8～1/6, the second pin of accumulated dose; The bad parent population for maturity, adopt three pins injections: the first pin be the pin that accelerates the ripening, and raun is injected LRH-A 21ug/kg, through 15～20d, be checked through raun abdominal distention and soft, can be hastened parturition, second and third pin is the pin of hastening parturition, and the water temperature of hastening parturition is in the time of 20～24 ℃, the needle gage of second and third pin is 8～12 h, and the oxytocic drug of second and third pin and consumption are: the every kg body weight of PG is 6～10mg; The every kg body weight of LRH-A2 is 3～5ug; The every kg body weight of HCG is 1000～1600IU, and the second pin injection is injected remaining all dosage for the 1/4～1/3, three pin of accumulated dose, and milter all adopts disposable injection, and dosage is 1/2 of raun accumulated dose;
(3) artificial insemination: hasten parturition after 12 h, parent population caught, checked, if raun can extrude the ovum grain, carry out artificial insemination immediately, if raun can not extrude the ovum grain, again parent population be checked every 2h, take the dry method insemination;
(4) adopt the hatching of miniflow water: the hatching water temperature is at 18～23 ℃, and in hatching process, apply 5ppm potassium permanganate soak at least 1min every day, and select in time bad ovum, the ovum of not inseminating, dead ovum;
(5) nursing of alevin stage: after fry is freely put down trip and opening, employing is carried out short cultivation in indoor miniflow water cement pond, yolk, the wheel animalcule after 80 order screen cloths filter of throwing something and feeding every day 2～4 times, its survival rate can reach more than 90%, grow to 1cm when above, start to forward to outdoor tank-culture and cultivate, and start to throw something and feed cladocera, copepoda, fry grows to 2cm when above, and the water earthworm starts to throw something and feed.
2. the former carp large-scale artificial of rock as claimed in claim 1 propagation method; it is characterized in that the dry method insemination is with dry towel, female, milter belly to be dried; first gently press the raun belly; fish-egg is bonded on attachment; rapidly the milter seminal fluid is directly clamp-oned again, by fish-egg and seminal fluid uniform stirring 0.8～1.2min gently, added Spawning water standing; wash away seminal fluid, during insemination, should avoid the sunlight direct irradiation.
CN2013104499122A 2013-09-29 2013-09-29 Scale artificial propagation method for procypris rabaudi Pending CN103461254A (en)

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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104012435A (en) * 2014-06-13 2014-09-03 临沂大学 Efficient breeding method for Rhodeus sinensis
CN104585094A (en) * 2015-01-16 2015-05-06 苏州市水产研究所有限公司 Method for breeding Xenocypris argentea Gunther artificially
CN104719218A (en) * 2015-04-10 2015-06-24 宁国市金东坊农业开发有限公司 Tor-brevifilis full-artificial early propagation method
CN104938385A (en) * 2015-06-23 2015-09-30 周天赐 Hemibarbus maculatus bleeker artificial reproduction method
CN104957054A (en) * 2015-05-28 2015-10-07 西双版纳傣族自治州水产技术推广站（渔政监督管理站） Tor sinensis artificial breeding method
CN105360035A (en) * 2015-12-03 2016-03-02 天津市金水族水产养殖有限公司 Ornamental carp breeding method
CN106035142A (en) * 2016-05-30 2016-10-26 铜陵东晟生态农业科技有限公司 Procypris rabaudi cultivation method
CN110876343A (en) * 2019-06-20 2020-03-13 四川省农业科学院水产研究所(四川省水产研究所) Method for improving artificial breeding efficiency of Leptobotia elongata

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CN102396653A (en) * 2011-11-09 2012-04-04 广东碧德生物科技有限公司 Granulated feed for procypris rabaudi

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CN102396653A (en) * 2011-11-09 2012-04-04 广东碧德生物科技有限公司 Granulated feed for procypris rabaudi

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Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104012435A (en) * 2014-06-13 2014-09-03 临沂大学 Efficient breeding method for Rhodeus sinensis
CN104012435B (en) * 2014-06-13 2015-12-30 临沂大学 China Bitterling Welfare (Rhodeus sinensis) efficient propagation method
CN104585094A (en) * 2015-01-16 2015-05-06 苏州市水产研究所有限公司 Method for breeding Xenocypris argentea Gunther artificially
CN104719218A (en) * 2015-04-10 2015-06-24 宁国市金东坊农业开发有限公司 Tor-brevifilis full-artificial early propagation method
CN104957054A (en) * 2015-05-28 2015-10-07 西双版纳傣族自治州水产技术推广站（渔政监督管理站） Tor sinensis artificial breeding method
CN104938385A (en) * 2015-06-23 2015-09-30 周天赐 Hemibarbus maculatus bleeker artificial reproduction method
CN105360035A (en) * 2015-12-03 2016-03-02 天津市金水族水产养殖有限公司 Ornamental carp breeding method
CN105360035B (en) * 2015-12-03 2018-03-30 天津市金水族水产养殖有限公司 The mating system of fancy carp
CN106035142A (en) * 2016-05-30 2016-10-26 铜陵东晟生态农业科技有限公司 Procypris rabaudi cultivation method
CN110876343A (en) * 2019-06-20 2020-03-13 四川省农业科学院水产研究所(四川省水产研究所) Method for improving artificial breeding efficiency of Leptobotia elongata
CN110876343B (en) * 2019-06-20 2022-02-08 四川省农业科学院水产研究所(四川省水产研究所) Method for improving artificial breeding efficiency of Leptobotia elongata

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Application publication date: 20131225