CN103446254A - Embedding method of buckwheat flavonoids - Google Patents
Embedding method of buckwheat flavonoids Download PDFInfo
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Abstract
The invention discloses an embedding method of buckwheat flavonoids. The embedding method comprises the following steps of: crushing tartary buckwheat seeds; digesting and purifying to obtain buckwheat flavonoids; preparing a mixed solution through buckwheat flavonoids, an emulsifying agent and an embedding agent; performing oscillating, mixing and embedding reaction; uniformly mixing; leaching through filter paper; and drying the filter paper after leaching to obtain a buckwheat flavonoids inclusion, wherein the embedding agent comprises one of or both beta-cyclodextrin and maltodextrin. According to the embedding method of the buckwheat flavonoids, the buckwheat flavonoids in the prepared embedding compound are high in embedding rate, reaching 53.41 to 91.42%. According to the evaluation on indicator quality of a buckwheat flavonoids microcapsule product prepared by the embedding method, the dissolvability is more than 90%, the product is greyish white and contains a little yellow powder, and the microcapsule has the size of about 5 to 25mu g.
Description
Technical field
The present invention relates to the Radix Et Rhizoma Fagopyri Tatarici composition and extract field, be specifically related to a kind of embedding method of Radix Et Rhizoma Fagopyri Tatarici flavone.
Background technology
Radix Et Rhizoma Fagopyri Tatarici, the formal name used at school hull buckwheat, produce in high and cold mountain area, in Southwestern China and Shanxi north one with plantation.Belong to Polygonaceae, the annual herb plant, high 30～60 centimetres.Stem is upright, the tool branch.Bottom leaf tool long handle, the wide triangular shape halberd of blade shape, full edge or wavy; The bottom leaf is less.The raceme axil is given birth to or Xiang Sheng, perianth white or rose pink.Pyrene is coniform avette, tool Rhizoma Sparganii, taupe.Flowering fruit bearing stage 6～JIUYUE.The seed of Radix Et Rhizoma Fagopyri Tatarici can be edible or be made feedstuff.Root and herb can be used as medicine, and energy removing damp and stopping pain, removing toxic substances and promoting subsidence of swelling, stomach invigorating cure mainly traumatic injury, lumbago and skelalgia, the generally acknowledged a kind of grain medicine dual-purpose grain kind of the international food and agricultural organization of sore and toxic ，Shi, are that the typical case of China's integration of edible and medicinal herbs culture embodies.
Current clinical medicine is observed and is shown, the Radix Et Rhizoma Fagopyri Tatarici mask has blood sugar lowering, blood fat reducing, strengthen body immunity, treat gastric disease, removing dampness and detoxicating, control nephritis, lose the effect of disliking meat in body, diabetes, hypertension, hyperlipidemia, coronary heart disease, apoplexy, patients with gastric disease are had to auxiliary therapeutic action.These effects are all relevant with the nutritional labeling contained in Radix Et Rhizoma Fagopyri Tatarici.Radix Et Rhizoma Fagopyri Tatarici is unique crop that integrates seven major nutrient in cereal crops, is described as " kings of grain rice ".Its seven major nutrient comprises:
Bioflavonoids: in Radix Et Rhizoma Fagopyri Tatarici, flavones content is abundant, as chromocor compound accounts for 3.05% of Radix Et Rhizoma Fagopyri Tatarici seed dry weight.The main component that rutin is Radix Et Rhizoma Fagopyri Tatarici flavone, account for 70% left and right of Radix Et Rhizoma Fagopyri Tatarici total flavonoids; Rutin can generate again Quercetin, two kinds of isomery variants of isoquercitin after hydrolysis.Research finds that rutin, Quercetin and isoquercitin have very high antioxidant activity, and free radical is had to very strong removing ability, therefore has stronger defying age and reaches the adjuvant treatment effect to various diseases.Separately studies have found that, contain a large amount of flavone compounds in bran of Radix Et Rhizoma Fagopyri Tatarici, it is a class alpha-glucosidase inhibitor, and effectively prevent diabetes occurs, and reduces blood sugar level, slows down the development of diabetes and alleviates diabetic complication.Because of unique pharmacological action and biological activity, Radix Et Rhizoma Fagopyri Tatarici flavone has obtained U.S.'s " state food and FAD " FDA authentication.
Trace element and mineral: inorganic elements calcium, phosphorus, ferrum, copper, zinc and trace element-selenium etc. that Radix Et Rhizoma Fagopyri Tatarici contains the multiple beneficial health, the content of magnesium is more than 11 times of Semen Tritici aestivi flour, ferrum element is 2～5 times of other staple food grain, zinc is more than 1.5 times, manganese is 1.4 to accompany abovely, and silicone content is higher more than 5 times than wheat flour, and lithium content is high more than 5 times, potassium is 2 times of Semen Tritici aestivi, and the high-load of magnesium, potassium has strengthened the alimentary health-care function of Radix Et Rhizoma Fagopyri Tatarici powder greatly.Because magnesium, potassium ion are to maintain water balance in body, the important cation of acid-base balance and osmotic pressure.Magnesium is the important element that various reactions are changed in participant's somatic cell, and potassium, magnesium is allaying tiredness effectively, stamina intensifying.Magnesium, potassium deficiency all will cause human body weak, and endurance descends.Magnesium deficiency also will make calcium produce harmful effect to brain, even cause angiopathy.Magnesium has the well-tuned effect to cardiac activity.Can make cardiac rhythm and the heart put forth energy to conduct and slow down, increase the blood supply of cardiac muscle amount, favourable diastole and rest.Can also promote human fiber's protein dissolution, the generation of Trombin inhibiting, reduce serum cholesterol, prevention of arterial sclerosis, hypertension, cardiopathic effect arranged, and calm nervous system is arranged, and strengthens the neural inhibit feature of old people Shen pivot simultaneously.These materials not only can improve the content of indispensable element in human body, also can play the effect that protects the liver kidney, hemopoietic and enhancing immunity, also are of value to raising intelligence, and the maintenance cardiovascular and cerebrovascular vessel are normal, the reduction cholesterol.In addition, also contain the selenium that other cereal crops lack in Radix Et Rhizoma Fagopyri Tatarici, be conducive to anti-cancer.
Starch: Radix Et Rhizoma Fagopyri Tatarici starch is amylopectin, containing a large amount of gel mucus, is alkalescence after heating, and hyperchlorhydria is had to inhibitory action, to focus, can play and alleviate and the barrier protection effect.
Vitamin: contain abundant vitamin in Radix Et Rhizoma Fagopyri Tatarici.Vitamin B1 can be promoted digestive function, neuritis and prevention foot disorder of QI.Vitamin B2 can promote the human body growth promoter, is the important component of prevention bicker, words, blepharitis.The content of vitamin B2 is Semen Maydis powder and rice 2～10 times.Can reduce blood capillary fragility and permeability, recover its elasticity.To preventing cerebral hemorrhage, maintaining the eye circulation, protection and enhancement vision are effective.
Cellulose: also claim dietary fiber, content reaches 1.6%, is more than the octuple of common rice and flour, has whole intestinal relieving constipation, removes the good effects of vivotoxin, is the street cleaner of digestion.
Fat: Radix Et Rhizoma Fagopyri Tatarici fatty acid composition mainly contains oleic acid, linoleic acid plus linolenic acid, be essential fatty acid, wherein oleic acid and linoleic acid content are high, and the linoleic acid most important fatty acid that is human body, in body, can not synthesize, the child is had to the enhancing development effect, to the adult, can prevent coronary heart disease.
Protein: contain 18 kinds of natural amino acids, total content is up to 11.82%.Contain especially general plant as the lysine that Semen Tritici aestivi, rice were lacked, be rich in arginine and histidine.And Radix Et Rhizoma Fagopyri Tatarici albumen has nearly 1/3rd for cleaning albumen, can clear up vivotoxin and foreign body.The content of aspartic acid, glutamic acid is high, and this two seed amino acid is all the important component that forms blood of human body (plasma protein).Aspartic acid is the analeptic of fine allaying tiredness, to athlete, heavy worker, old people, is all good health-care agent.
At present, also ripe gradually to the research of Radix Et Rhizoma Fagopyri Tatarici, the present invention mainly is to provide a kind of embedding method of Radix Et Rhizoma Fagopyri Tatarici flavone.
Summary of the invention
The object of the present invention is to provide a kind of embedding method of Radix Et Rhizoma Fagopyri Tatarici flavone, the embedding rate of its Radix Et Rhizoma Fagopyri Tatarici flavone is high.
For achieving the above object, the present invention by the following technical solutions: a kind of embedding method of Radix Et Rhizoma Fagopyri Tatarici flavone, its concrete operations are as follows:
S1: by after the fragmentation of Radix Et Rhizoma Fagopyri Tatarici seed, carrying out extraction and purification, obtain Radix Et Rhizoma Fagopyri Tatarici flavone;
S2: get Radix Et Rhizoma Fagopyri Tatarici flavone and emulsifying agent and embedding medium and be configured to mixed solution and shake and mix the embedding reaction, mix and with filter paper, carry out sucking filtration afterwards, after sucking filtration, filter paper is carried out can obtaining the Radix Et Rhizoma Fagopyri Tatarici flavone inclusion after drying;
Described embedding medium is one or both formations in beta-schardinger dextrin-, maltodextrin.
Further scheme is:
Described emulsifying agent is soybean lecithin.
Described embedding medium is beta-schardinger dextrin-, and Radix Et Rhizoma Fagopyri Tatarici flavone is mixed according to mass ratio (ratio of core wall material) 1:5～25 with beta-schardinger dextrin-.
The temperature that concussion mixes the embedding reaction is 25～50 ℃, and the time is 0.5～2h, 5% of the gross mass that the addition of soybean lecithin is beta-schardinger dextrin-and Radix Et Rhizoma Fagopyri Tatarici flavone.
In S1, extraction and purification comprises the Radix Et Rhizoma Fagopyri Tatarici powder after the fragmentation of Radix Et Rhizoma Fagopyri Tatarici seed and cellulase is carried out to enzyme digestion reaction, enzyme digestion reaction carries out the Hybrid Heating lixiviate by enzymolysis solution and alcoholic solution after finishing, after the heating lixiviate, mixed liquor is carried out to centrifugation, get supernatant liquid and carry out evaporation and concentration, carry out after evaporation and concentration can obtaining Radix Et Rhizoma Fagopyri Tatarici flavone dry powder after lyophilization.
In the heating lixiviate, alcoholic solution adds the alcoholic solution of 100～150ml according to every 10g Radix Et Rhizoma Fagopyri Tatarici powder, and the concentration of alcoholic solution is 50～90%, and the heating lixiviate is operating as in the temperature range of 45～90 ℃ reflux 2 times, and 0.5～2h refluxes at every turn.
Highly preferred scheme is:
The temperature of enzyme digestion reaction is 52 ℃, and pH value is 6.8, and enzymolysis time is 90min, and the concentration of enzyme digestion reaction mixed liquor cellulase is 2.5～2.8ul/L.
The temperature that concussion mixes the embedding reaction is 40 ℃, and the time is 2h, and Radix Et Rhizoma Fagopyri Tatarici flavone is mixed according to mass ratio 1:10 with beta-schardinger dextrin-.
The concentration of alcoholic solution is 80%, and the temperature of heating lixiviate operation is 80～90 ℃, and 2h refluxes at every turn.
The embedding method of Radix Et Rhizoma Fagopyri Tatarici flavone is provided by such scheme in the present invention, and its embedding rate of producing the Radix Et Rhizoma Fagopyri Tatarici flavone in the embedding compound obtained is high, can reach 53.41～91.42%.The index ' s quality evaluation result of the Radix Et Rhizoma Fagopyri Tatarici flavone microcapsule product made by this embedding method is: dissolubility is greater than 90%, and product is greyish white, yellow powder slightly, and the microcapsule size is between 5～25 μ g.
The specific embodiment
Below come by specific embodiment that the invention will be further described, wherein embodiment 1 is for producing Radix Et Rhizoma Fagopyri Tatarici flavone, embodiment 2 is for to utilize the Radix Et Rhizoma Fagopyri Tatarici flavone of producing in embodiment 1 to produce clathrate.
Wherein, in embodiment 1,2, the assay method of flavones content is:
The drafting of rutin standard curve: the rutin standard solution is carried out to full wavelength scanner at 200～800nm, and at the 430nm wavelength, there is maximum absorption band at place, therefore detect wavelength, is chosen as 430nm.Accurately take rutin standard substance 100.00mg, with 60% ethanol, be settled to 100mL as standard solution, shake up, draw respectively titer 0.4mL, 0.8mL, 1.2mL, 1.6mL, 2.0mL in 10mL tool plug test tube, with 60% ethanol, be added into 4mL, add 5% sodium nitrite solution 0.4mL, shake up, place 6min, after add 10% aluminum nitrate 0.4mL, add 4% sodium hydroxide 4mL after 6min, shake up, then be settled to scale with 30% ethanol, shake up, place 15min, survey absorbance at the 430nm place.Make reference solution with reagent blank.
The mensuration of tartary buckwheat extract general flavone content: accurately take a certain amount of Radix Et Rhizoma Fagopyri Tatarici flavone extract, be configured to certain density solution, by above-mentioned standard curve method, measure its absorbance, thereby push away to obtain its general flavone content.
In embodiment 2 in clathrate the method for flavones content and micro encapsulation efficiency test be:
The mensuration of clathrate surface flavones content: accurately take a certain amount of clathrate, add dehydrated alcohol, fully after the vibration washing, be settled to certain volume, filter, by above-mentioned Study of Flavonoids Determination in Soya, flavones content is measured.
The mensuration of general flavone content in clathrate: accurately take a certain amount of clathrate, add 50% alcoholic solution, fully after the vibration washing, be settled to certain volume, filter, by above-mentioned Study of Flavonoids Determination in Soya, flavones content is measured.
The mensuration of micro encapsulation efficiency: micro encapsulation efficiency (%)=(the core content in 1～product surface core content/product) * 100
In embodiment 1,2, experimental apparatus used, material are respectively:
Embodiment 1
A1: get Radix Et Rhizoma Fagopyri Tatarici powder 10g, put into the 250mL beaker, add cellulase to carry out enzyme digestion reaction, the temperature of controlled enzymatic hydrolysis reaction is 52 ℃, and pH value is 6.8, and enzymolysis time is 90min, and the concentration of enzyme digestion reaction mixed liquor cellulase is 2.5～2.8ul/L.Enzyme digestion reaction adds 80% alcoholic solution 150mL after finishing, by ultrasonic method, extract, extracting temperature is 60 ℃, ultrasonic time 30min, then carry out vacuum filtration, obtain filtrate and filtering residue, filtering residue is extracted once with same method again, merge twice filtrate after sucking filtration, and supernatant rotary evaporation to 10～20mL postlyophilization will be got after its centrifugation, obtain dry powder, calculate extraction ratio, and measure flavones content.
A2: get Radix Et Rhizoma Fagopyri Tatarici powder 15g, put into the 250mL flask, add cellulase to carry out enzyme digestion reaction, the temperature of controlled enzymatic hydrolysis reaction is 52 ℃, and pH value is 6.8, and enzymolysis time is 90min, and the concentration of enzyme digestion reaction mixed liquor cellulase is 2.5～2.8ul/L.Enzyme digestion reaction adds certain density alcoholic solution 150mL after finishing, hot reflux at a certain temperature 2 times, a 2h., by getting supernatant rotary evaporation to 10～20mL postlyophilization after extracting solution centrifugation, obtains dry powder, calculate extraction ratio, and with measuring flavones content.
Result: the extraction ratio of alcohol steep method is 6.96%, and flavones content is 84.52%, and extraction ratio and flavones content are all very high, and extract is easy to concentrated and dry.The extraction ratio of ultrasonic extraction is 5.31%, and flavones content is 73.46%, and this kind of method is larger to the loss of equipment, and the extraction effect that is not so good as the alcohol steep method is good.In sum, the alcohol steep method is the optimum extraction scheme.
A3: the extraction of Radix Et Rhizoma Fagopyri Tatarici flavone experiment under different ethanol concentration
The alcoholic solution 100mL that to get respectively concentration be 50%, 60%, 70%, 80%, 90%, add respectively wherein 10g Radix Et Rhizoma Fagopyri Tatarici powder, in 80～90 ℃ of temperature ranges, hot reflux is 2 times, one time 2h. will get supernatant rotary evaporation to 10～20mL postlyophilization after extracting solution centrifugation, obtain dry powder, calculate extraction ratio, and, with measuring flavones content, result is as shown in table 1:
Radix Et Rhizoma Fagopyri Tatarici flavone extraction ratio under table 1 different ethanol concentration
A4: different extraction experiments of extracting Radix Et Rhizoma Fagopyri Tatarici flavone at temperature
Get Radix Et Rhizoma Fagopyri Tatarici powder 15g, put into the 250mL flask, add 80% alcoholic solution 150mL, hot reflux 2 times at 45 ℃, 55 ℃, 65 ℃, 75 ℃, 85 ℃ temperature respectively, a 2h., by getting supernatant rotary evaporation to 10～20mL postlyophilization after extracting solution centrifugation, obtains dry powder, calculates extraction ratio, and, with measuring flavones content, result is as shown in table 2:
The different Radix Et Rhizoma Fagopyri Tatarici flavone extraction ratios that extract at temperature of table 2
A5: the extraction experiment of Radix Et Rhizoma Fagopyri Tatarici flavone under different extraction times
Get Radix Et Rhizoma Fagopyri Tatarici powder 15g, put into the 250mL flask, add 80% alcoholic solution 150mL, in 80～90 ℃ of temperature ranges, hot reflux is 2 times, and each time of refluxing is respectively 0.5h, 1h, 1.5h, 2h.By getting supernatant rotary evaporation to 10～20mL postlyophilization after extracting solution centrifugation, obtain dry powder, calculate extraction ratio, and, with measuring flavones content, result is as shown in table 3:
The Radix Et Rhizoma Fagopyri Tatarici flavone extraction ratio of table 3 under different extraction times
Along with the increase of concentration of alcohol used, the extraction ratio of Radix Et Rhizoma Fagopyri Tatarici flavone increases gradually as can be seen from Table 1, but, when concentration of alcohol reaches 80%, extraction rate reached is to maximum.Concentration of alcohol continues to increase extraction ratio and descends.Along with the increase of extracting temperature, the extraction ratio of Radix Et Rhizoma Fagopyri Tatarici flavone increases gradually, until reach the boiling point of alcoholic solution, is the optimum extraction temperature as can be seen from Table 2.Along with the increase of extraction time, the extraction ratio of Radix Et Rhizoma Fagopyri Tatarici flavone increases gradually as can be seen from Table 3.
Therefore the optimal conditions of determining Radix Et Rhizoma Fagopyri Tatarici extraction flavone are: 80～90 ℃ of temperature of extraction, extraction time 2h, extracting solution concentration of alcohol used are 80%.
Embodiment 2
B1: accurately take appropriate beta-schardinger dextrin-, a certain amount of Radix Et Rhizoma Fagopyri Tatarici flavone, put into the 250mL beaker, add the 100mL distilled water, constant temperature oscillation certain hour under the uniform temperature condition, cooling rear sucking filtration, put into the thermostatic drying chamber inner drying by the material obtained on filter paper and obtain clathrate.
B2: accurately take appropriate maltodextrin, a certain amount of Radix Et Rhizoma Fagopyri Tatarici flavone, put into the 250mL beaker, add the 100mL distilled water, constant temperature oscillation certain hour under the uniform temperature condition, cooling rear sucking filtration, put into the thermostatic drying chamber inner drying by the material obtained on filter paper and obtain clathrate.
B3: accurately take appropriate hydroxypropyl powder-beta-dextrin, a certain amount of Radix Et Rhizoma Fagopyri Tatarici flavone, put into the 250mL beaker, add the 100mL distilled water, constant temperature oscillation certain hour under the uniform temperature condition, cooling rear sucking filtration, put into the thermostatic drying chamber inner drying by the material obtained on filter paper and obtain clathrate.
Experimental result to B1～B3 compares, and by the known beta-schardinger dextrin-of comparative result, is best wall material, and the dissolubility of hydroxypropyl powder-beta-dextrin is excessive, undesirable.
B4: the impact of the comparison embedding rate of core wall material
Accurately take respectively beta-schardinger dextrin-5g, 10g, 15g, 20g, 25g, add respectively wherein Radix Et Rhizoma Fagopyri Tatarici flavone 1g, put into the 250mL beaker, add the 100mL distilled water, and add 0.5% soybean lecithin as emulsifying agent, constant temperature oscillation 2h under 40 ℃ of conditions, cooling rear sucking filtration, put into the thermostatic drying chamber inner drying by the material obtained on filter paper and obtain clathrate.Measure flavones content in clathrate, result is as shown in table 4;
The different core wall of table 4 material than under embedding rate
B5: the impact of embedding temperature on embedding rate
Accurately take beta-schardinger dextrin-10g, Radix Et Rhizoma Fagopyri Tatarici flavone 1g, put into the 250mL beaker, add the 100mL distilled water, and add 0.5% soybean lecithin as emulsifying agent, constant temperature oscillation 2h under 25 ℃, 30 ℃, 35 ℃, 40 ℃, 45 ℃, 50 ℃ conditions, cooling rear sucking filtration, put into the thermostatic drying chamber inner drying by the material obtained on filter paper and obtain clathrate.Measure flavones content in clathrate, result is as shown in table 5;
Embedding rate at the different embedding temperature of table 5
B6: the impact of embedding time on embedding rate
Accurately take beta-schardinger dextrin-10g, Radix Et Rhizoma Fagopyri Tatarici flavone 1g, put into the 250mL beaker, add the 100mL distilled water, and add 0.5% soybean lecithin as emulsifying agent, difference constant temperature oscillation 0.5h, 1h, 1.5h, 2h under 40 ℃ of conditions, cooling rear sucking filtration, put into the thermostatic drying chamber inner drying by the material obtained on filter paper and obtain clathrate.Measure flavones content in clathrate, result is as shown in table 6;
The embedding rate of table 6 under the different embedding time
The impact of the embedding effect of the comparison Radix Et Rhizoma Fagopyri Tatarici flavone of core wall material is very large as can be seen from Table 4, embedding rate maximum when its value is 1:10.Along with the increase of embedding temperature, the extraction ratio of Radix Et Rhizoma Fagopyri Tatarici flavone increases gradually as can be seen from Table 5, and when the embedding temperature is that 40 ℃ of embedding rates reach the highest, along with the increase of embedding temperature, embedding rate descends gradually afterwards.Along with the increase of embedding time, the embedding rate of Radix Et Rhizoma Fagopyri Tatarici flavone increases gradually as can be seen from Table 6.When the embedding time is 1.5h, embedding rate reaches maximum.Therefore best embedding conditions is: embedding time 2h, and 40 ℃ of embedding temperature, the ratio 1:10 of core wall material, emulsifying agent is soybean lecithin, addition is 0.5%.
The index ' s quality evaluation result of the Radix Et Rhizoma Fagopyri Tatarici flavone microcapsule product made by this embedding method is: dissolubility is greater than 90%, and product is greyish white, yellow powder slightly, and the microcapsule size is between 5～25 μ g.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any modifications of doing within the spirit and principles in the present invention, be equal to and replace and improvement etc., within all should being included in protection scope of the present invention.
Claims (9)
1. the embedding method of a Radix Et Rhizoma Fagopyri Tatarici flavone, its concrete operations are as follows:
S1: by after the fragmentation of Radix Et Rhizoma Fagopyri Tatarici seed, carrying out extraction and purification, obtain Radix Et Rhizoma Fagopyri Tatarici flavone;
S2: get Radix Et Rhizoma Fagopyri Tatarici flavone and emulsifying agent and embedding medium and be configured to mixed solution and shake and mix the embedding reaction, mix and with filter paper, carry out sucking filtration afterwards, after sucking filtration, filter paper is carried out can obtaining the Radix Et Rhizoma Fagopyri Tatarici flavone inclusion after drying;
Described embedding medium is one or both formations in beta-schardinger dextrin-, maltodextrin.
2. the embedding method of Radix Et Rhizoma Fagopyri Tatarici flavone as claimed in claim 1, it is characterized in that: described emulsifying agent is soybean lecithin.
3. the embedding method of Radix Et Rhizoma Fagopyri Tatarici flavone as claimed in claim 1 or 2, it is characterized in that: described embedding medium is beta-schardinger dextrin-, Radix Et Rhizoma Fagopyri Tatarici flavone is mixed according to mass ratio 1: 5～25 with beta-schardinger dextrin-.
4. the embedding method of Radix Et Rhizoma Fagopyri Tatarici flavone as claimed in claim 3 is characterized in that: the temperature that concussion mixes the embedding reaction is 25～50 ℃, and the time is 0.5～2h, 5% of the gross mass that the addition of soybean lecithin is beta-schardinger dextrin-and Radix Et Rhizoma Fagopyri Tatarici flavone.
5. the embedding method of Radix Et Rhizoma Fagopyri Tatarici flavone as claimed in claim 1, it is characterized in that: in S1, extraction and purification comprises the Radix Et Rhizoma Fagopyri Tatarici powder after the fragmentation of Radix Et Rhizoma Fagopyri Tatarici seed and cellulase is carried out to enzyme digestion reaction, enzyme digestion reaction carries out the Hybrid Heating lixiviate by enzymolysis solution and alcoholic solution after finishing, after the heating lixiviate, mixed liquor is carried out to centrifugation, get supernatant liquid and carry out evaporation and concentration, carry out after evaporation and concentration can obtaining Radix Et Rhizoma Fagopyri Tatarici flavone dry powder after lyophilization.
6. the embedding method of Radix Et Rhizoma Fagopyri Tatarici flavone as claimed in claim 5, it is characterized in that: the temperature of enzyme digestion reaction is 52 ℃, and pH value is 6.8, and enzymolysis time is 90min, and the concentration of enzyme digestion reaction mixed liquor cellulase is 2.5～2.8ul/L.
7. the embedding method of Radix Et Rhizoma Fagopyri Tatarici flavone as claimed in claim 5, it is characterized in that: in the heating lixiviate, alcoholic solution adds the alcoholic solution of 100～150ml according to every 10g Radix Et Rhizoma Fagopyri Tatarici powder, the concentration of alcoholic solution is 50～90%, the heating lixiviate is operating as in the temperature range of 45～90 ℃ reflux 2 times, and 0.5～2h refluxes at every turn.
8. the embedding method of Radix Et Rhizoma Fagopyri Tatarici flavone as claimed in claim 4 is characterized in that: the temperature that concussion mixes the embedding reaction is 40 ℃, and the time is 2h, and Radix Et Rhizoma Fagopyri Tatarici flavone is mixed according to mass ratio with beta-schardinger dextrin-at 1: 10.
9. the embedding method of Radix Et Rhizoma Fagopyri Tatarici flavone as claimed in claim 7, it is characterized in that: the concentration of alcoholic solution is 80%, and the temperature of heating lixiviate operation is 80～90 ℃, and 2h refluxes at every turn.
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| CN111150087A (en) * | 2019-12-31 | 2020-05-15 | 西昌市正中食品有限公司 | Embedding wall material and microcapsule tartary buckwheat extract and preparation process thereof |
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| CN111328943A (en) * | 2020-02-28 | 2020-06-26 | 西昌市正中食品有限公司 | Tartary buckwheat active peptide beverage and preparation process thereof |
| CN113230314A (en) * | 2021-05-31 | 2021-08-10 | 云南八凯农业开发有限公司 | Method for extracting tartary buckwheat flavone |
| CN116268395A (en) * | 2022-06-17 | 2023-06-23 | 贵州省生物技术研究所(贵州省生物技术重点实验室、贵州省马铃薯研究所、贵州省食品加工研究所) | Preparation method of instant powder microcapsule for tartary buckwheat sprouts |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101366830A (en) * | 2008-09-25 | 2009-02-18 | 成都大学 | Tartary buckwheat biological flavone effervescent tablet and preparation method thereof |
| CN102334685A (en) * | 2011-05-27 | 2012-02-01 | 陕西科技大学 | Preparation method of buckwheat flavone microcapsule and its product |
| CN102783647A (en) * | 2012-08-28 | 2012-11-21 | 句容茅宝葛业有限公司 | Preparation method of kudzu root isoflavone powder |
| CN102948904A (en) * | 2011-08-25 | 2013-03-06 | 河北农业大学 | Production technology of high-calcium tartary buckwheat flavone instant beverage |
| CN102948758A (en) * | 2012-11-26 | 2013-03-06 | 陕西科技大学 | Method for extracting buckwheat flavone from buckwheat bran |
-
2013
- 2013-08-13 CN CN201310355787.9A patent/CN103446254B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101366830A (en) * | 2008-09-25 | 2009-02-18 | 成都大学 | Tartary buckwheat biological flavone effervescent tablet and preparation method thereof |
| CN102334685A (en) * | 2011-05-27 | 2012-02-01 | 陕西科技大学 | Preparation method of buckwheat flavone microcapsule and its product |
| CN102948904A (en) * | 2011-08-25 | 2013-03-06 | 河北农业大学 | Production technology of high-calcium tartary buckwheat flavone instant beverage |
| CN102783647A (en) * | 2012-08-28 | 2012-11-21 | 句容茅宝葛业有限公司 | Preparation method of kudzu root isoflavone powder |
| CN102948758A (en) * | 2012-11-26 | 2013-03-06 | 陕西科技大学 | Method for extracting buckwheat flavone from buckwheat bran |
Non-Patent Citations (2)
| Title |
|---|
| 刘文彦: "甜荞麦黄酮类化合物微胶囊化保存技术的研究", 《中国优秀硕士学位论文全文数据库（电子期刊）》, no. 2, 1 March 2013 (2013-03-01), pages 024 - 104 * |
| 孙艳华: "酶法提取荞麦茎叶中黄酮的研究", 《食品工业》, no. 3, 20 June 2006 (2006-06-20), pages 16 - 17 * |
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| CN104306463A (en) * | 2014-11-06 | 2015-01-28 | 云南谷冠生物科技有限公司 | Method for preparing buckwheat total flavone from buckwheat leftovers and application thereof |
| CN104306463B (en) * | 2014-11-06 | 2017-08-15 | 云南谷冠生物科技有限公司 | A kind of method and its application that bitter buckwheat general flavone is prepared with bitter buckwheat leftover bits and pieces |
| CN109123618A (en) * | 2018-06-01 | 2019-01-04 | 广东展翠食品股份有限公司 | A kind of manufacture craft of fingered citron flavones chewable tablets |
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| CN111150086A (en) * | 2019-12-31 | 2020-05-15 | 西昌市正中食品有限公司 | Embedding wall material, and embedding process and application of microcapsule tartary buckwheat flavone |
| CN111150085A (en) * | 2019-12-31 | 2020-05-15 | 西昌市正中食品有限公司 | Composite embedding wall material, microcapsule tartary buckwheat nutrient and preparation process |
| CN111150087A (en) * | 2019-12-31 | 2020-05-15 | 西昌市正中食品有限公司 | Embedding wall material and microcapsule tartary buckwheat extract and preparation process thereof |
| CN111150086B (en) * | 2019-12-31 | 2022-02-18 | 西昌市正中食品有限公司 | Embedding wall material, and embedding process and application of microcapsule tartary buckwheat flavone |
| CN111150087B (en) * | 2019-12-31 | 2022-03-18 | 西昌市正中食品有限公司 | Microcapsule containing tartary buckwheat extract and preparation process thereof |
| CN111150085B (en) * | 2019-12-31 | 2022-05-13 | 西昌市正中食品有限公司 | Composite embedding wall material, microcapsule tartary buckwheat nutrient and preparation process |
| CN111329045A (en) * | 2020-02-28 | 2020-06-26 | 西昌市正中食品有限公司 | Three-high reducing tartary buckwheat coarse cereal powder |
| CN111328943A (en) * | 2020-02-28 | 2020-06-26 | 西昌市正中食品有限公司 | Tartary buckwheat active peptide beverage and preparation process thereof |
| CN113230314A (en) * | 2021-05-31 | 2021-08-10 | 云南八凯农业开发有限公司 | Method for extracting tartary buckwheat flavone |
| CN113230314B (en) * | 2021-05-31 | 2022-05-03 | 云南八凯农业开发有限公司 | Method for extracting tartary buckwheat flavone |
| CN116268395A (en) * | 2022-06-17 | 2023-06-23 | 贵州省生物技术研究所(贵州省生物技术重点实验室、贵州省马铃薯研究所、贵州省食品加工研究所) | Preparation method of instant powder microcapsule for tartary buckwheat sprouts |
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