CN101199581B - Chick-pea embryo and bean sprout valid part, preparing method and application thereof - Google Patents
Chick-pea embryo and bean sprout valid part, preparing method and application thereof Download PDFInfo
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Abstract
The invention relates to a chickpea germ, effective part of bean sprout, a preparation method and application. The effective part of the total saponin and isoflavone can be obtained from the chickpea germ and bean sprout by adopting an alcohol extraction method or a water extraction method; the initial screening test of the effective part proves that: the effective part has the advantages of playing a role as an inhibitor for protein tyrosine phosphatase (PTP1B). The effective part can be used as a medicine for treating diabetes and as an application of health care product, simultaneously, the effective part or the derivative of which can be applied as a drug combination.
Description
Technical field
The present invention relates to a kind ofly from chick-pea embryo and bean sprout or exsiccant chick-pea embryo powder and bean sprout powder, extract effective site, this effective site is as the hypoglycemic purposes of preparation treatment, especially to the purposes of treatment type 2 diabetes mellitus.
Background technology
Plant extract is an emerging industry; From the just time of short more than ten years occurring till now; But start the overbearing tide of one " back to nature " in the whole world; People are feeling the attaching after the effect of chemical synthetic drug, the Chinese herbal medicine of pure natural is produced a kind of security, thereby make that the development of plant extract is swift and violent and irresistible.Plant extract market is closely bound up with plant amedica market, and the plant amedica market in the whole world in 2005 is 26,000,000,000 dollars, wherein Europe: 34.5% (Germany, France have accounted for 65%), and the North America: 21%, the Asia: 26%, Japan: 11.3%.Plant amedica market development speed in the whole world is 10%～20%, and global plant extract market development speed is 15%～20%.Plant extract main activity country is countries such as the U.S., Europe (wherein Germany, France the most active), China, India, Korea S, Japan, Brazil.The U.S. is the country of the last approval in whole world plant amedica, but the plant extract consumption of the U.S. has accounted for more than 60% of the whole world at present.American plant extract consumption was about about 2,800,000,000 dollars in 2005, and U.S.'s " food complete works of " is investigated 2000 tame health food stores, and the result shows with extract and accounts for 7.4% as type of service.1992, the sales volume of american plant medicine was 1,200,000,000 dollars, reached 7,000,000,000 dollars by 2005, and the average year growth rate surpasses 10%.Herb extracts market, Asia in 2005 is 1,500,000,000 dollars, and about 400,000,000 dollars of China accounts for about 26.7%; Japan is 3.2 hundred million dollars, accounts for 21%.Thus it is clear that, the development further that awaits of Chinese Plants medicine.At present, the plant extract kind is about more than 80 kinds, and the natural plant extracts of North America market mainly contains Semen Ginkgo extrac---Semen Ginkgo pyrite, green tea extract---tea polyphenols, kawa extract---Kava Kava etc.Domestic natural plant extracts is of a great variety, and existing kind can be divided into 3 types: the single medicinal material extract, like extracts such as Fructus Aurantii Immaturus, Radix Angelicae Sinensis, the Radix Astragali, Fructus Schisandrae Chinensis, Ganoderma, Fructus Tribuli, Cortex Magnoliae Officinalis, Radix Et Caulis Acanthopanacis Senticosi, Herba Hyperici perforati, Herba Trifolii Pratentis, Folium Ginkgos; The herbal mixture extract is like invigorating the spleen and replenishing QI formula extraction etc.; Purified extract comprises active site and monomeric compound, and is first-class like soybean isoflavone, ginsenoside, tea catechin, resveratrol, huperzine.
Chickpea (Cicer arietinum L.) originates from the West Asia, Mediterranean is littoral, and existing 1.5 hundred million mu of existing global sown area is second largest in the world at present edible legume crop; Uygur nationality's promise by name is carried recklessly, has growth in 2500 historical in Xinjiang, is the Uygur medicine medicinal herbs most in use; Recorded in Ministry of Health of the People's Republic of China's " drug standard " Uigurs medicine fascicle and " Uigurs medicine will "; Have the unusual body fluid of removing, open the body fluid impatency, regulate effects such as body.Be used for asthenic body, hyposexuality, inappetence, diseases such as skin pruritus and diabetes.
Chickpea nutritious composition is than horn of plenty: the dried bean of per 100 grams contains moisture 10 grams, crude protein 23 grams, starch 47.3 grams, soluble sugar 5.8 grams, fat 5.3 grams, crude fibre 6.3 grams, ash 3.2 grams approximately.Chickpea contains abundant trace element, and wherein vitamin content reaches 42 milligrams, is higher than most of bean class; The content of ferrum reaches 47 milligrams; Exceed 90% than other beans, this makes edible chickpea product can effectively reduce the human serum lipid level, reduces accumulation of cholesterol; Strengthen middle-aged and elderly people central nervous system's adjusting, hypertension, heart disease are had tangible preventive effect.Show through comparative study: chickpea, Semen sojae atricolor, Semen phaseoli radiati etc. are on protein efficiency ratio, biological value and digestibility index, and chickpea is for the highest.The famous medical expert of the U.S. restrained the Lay Ernest & Whitney through the lots of clinical experience in 1956, had confirmed that chickpea has tangible curative effect to more than 70 kinds of severe malnutrition diseases.
The chickpea medical value is also confirmed by Institute of Science: have effects such as building body, memory reinforcing, treatment prevent diabetes.In addition, the chickpea seed can also be prevented and treated gallbladder disease, can make diuretic, galactopoietic, Cure for insomnia, prevention dermatosis etc.Available data is analyzed; Chickpea has good blood sugar lowering and effect for reducing blood fat to the hyperglycemia mice; Can reduce the concentration of blood glucose in the serum, insulin and triglyceride, strengthen the activity of glucokinase, the prompting chickpea is being played the part of important role as functional food in diabetes control.Research shows that also discovery chickpea ethanol such as chickpea has good active anticancer, Giron-Calle J and acetone extraction part almost can suppress the growth of tumor cell fully, show good antineoplastic activity.Discover that compositions such as isoflavone in the chickpea, Saponin class have blood fat reducing function preferably, and these compositions concentrate on mainly in the chick-pea embryo, therefore, the inventor has carried out hypoglycemic research to the chickpea bean sprout.The stomach method of irritating is adopted in research; Discover that chickpea bean sprout extract has tangible blood sugar reducing function to alloxan hyperglycemia model mice; Though the model group animal is along with time lengthening, blood glucose also reduces gradually, and the blood glucose decline percentage rate of administration group and its more all have the difference of highly significant.
Because the incidence and development of type 2 diabetes mellitus can cause by the interaction of h and E factor etc., wherein, the important function that insulin signaling transduction obstacle is risen in pathogenesis.PTP 1B (Protein tyrosine phosphatase PTP1B) is crucial negative regulation of insulin signaling transduction.PTP1B is the representative of PTP in the born of the same parents; It is first mammalian proteins tyrosine phosphatase of being identified and being purified into; Form by 435 amino acid residues; The about 50KD of molecular weight, PTP 1B be ubiquity and expression in each histiocyte of human body, and through making activated Insulin receptor INSR and IRS dephosphorylation inactivations such as (IRs-1) bring into play physiological function.Balance between its tunable tyrosine residue phosphorylation and the dephosphorylation stops the insulin signaling transduction.Protein-tyrosine-phosphatase content and activity special in type 2 diabetes mellitus and the insulin resistant patient body all increase to some extent, and after Protein-tyrosine-phosphatase was disallowable, not only insulin sensitivity increased, and diabetic symptom also makes moderate progress.The mice that PTP 1B lacks and its wild type than in control mice are more responsive to insulin; Glycemic control is better; The mice that lacks and its wild type than in control mice are more responsive to insulin, and glycemic control is better, and the obesity of diet induced is had better resistant function.Therefore, the effect of CKIs tyrosine phosphatase 1B has become a kind of new treatment insulin resistant (IR), type ii diabetes and fat method.
Summary of the invention
The object of the invention is; A kind of effective site that adopts solvent extraction method from fresh chickpea bean embryo, bean sprout or exsiccant chick-pea embryo powder, bean sprout powder, to obtain total saponins class, osajin is provided; This effective site is through preliminary screening active ingredients evidence: have the effect of Protein-tyrosine-phosphatase (PTP1B) inhibitor; This effective site is used to treat the application of the medicine or the health product of diabetes, and this effective site or derivatives thereof can be used as the application of pharmaceutical composition simultaneously.
Employing solvent extraction method of the present invention (alcohol extracting method or water extraction) preparation chick-pea embryo and bean sprout valid part and its production and application, containing isoflavone and saponin constituent in this effective site, concrete operations follow these steps to carry out:
A, chickpea is germinateed with conventional method;
B, with the methanol or ethanol or isopropyl alcohol or the butanols that add 2-10 times of volume in fresh chick-pea embryo and bean sprout or exsiccant chick-pea embryo powder and the bean sprout powder, its concentration is 10-100%.
C, with ultrasound wave or merceration or heating and refluxing extraction 1-15 time, each 1-3 hour, wherein the ultrasonic extraction temperature was 20-40 ℃, the reflux, extract, temperature is 60-80 ℃, use filter paper filtering, merging filtrate, being evaporated to does not have alcohol and distinguishes the flavor of, and obtains ethanol extract effective site;
D or with the pure water that adds 2-10 times of volume in fresh chick-pea embryo and bean sprout or exsiccant chick-pea embryo powder and the bean sprout powder;
E, with ultrasound wave or merceration or heating and refluxing extraction 1-15 time; Each 2-4 hour, wherein the ultrasonic extraction temperature was 20-60 ℃, and the reflux, extract, temperature is 60-100 ℃ and uses gauze, filter paper filtering respectively; Merging filtrate, concentrating under reduced pressure obtain water extract effective site.
Said effective site is total saponins class and osajin.
Effective site is prepared as functional food or processes capsule or tablet or oral liquid or electuary or injection or slow releasing preparation or controlled release preparation by conventional pharmaceutical methods by conventional method; Or with effective site with in, the Western medicine compatibility processes capsule or tablet or oral liquid or electuary or injection or slow releasing preparation or controlled release preparation.
The method for preparing of a kind of chick-pea embryo and bean sprout valid part, this effective site adopt alcohol extracting method or water extraction preparation, and concrete operations follow these steps to carry out:
A, chickpea is germinateed with conventional method;
B, with the methanol or ethanol or isopropyl alcohol or the butanols that add 2-10 times of volume in fresh chick-pea embryo and bean sprout or exsiccant chick-pea embryo powder and the bean sprout powder, its concentration is 10-100%.
C, with ultrasound wave or merceration or heating and refluxing extraction 1-15 time, each 1-3 hour, wherein the ultrasonic extraction temperature was 20-40 ℃, the reflux, extract, temperature is 60-80 ℃, use filter paper filtering, merging filtrate, being evaporated to does not have alcohol and distinguishes the flavor of, and obtains ethanol extract effective site;
D or with the pure water that adds 2-10 times of volume in fresh chick-pea embryo and bean sprout or exsiccant chick-pea embryo powder and the bean sprout powder;
E, with ultrasound wave or merceration or heating and refluxing extraction 1-15 time, each 2-4 hour, wherein the ultrasonic extraction temperature was 20-60 ℃; The reflux, extract, temperature is 60-100 ℃; Use gauze, filter paper filtering respectively, merging filtrate, concentrating under reduced pressure obtain water extract effective site.
Described method for preparing, step b alcohol optium concentration is 60-70%
Described method for preparing, step c alcohol ultrasonic extraction temperature is preferably 30-35 ℃, and the reflux, extract, temperature is preferably 70-80 ℃.
Described method for preparing, the ultrasonic extraction temperature is preferably 30-40 ℃ among the step e, and the reflux, extract, temperature is preferably 80-96 ℃.
A kind of chick-pea embryo and bean sprout valid part are as the purposes of preparation treatment diabetes hypoglycemic drug or as the purposes of food with function of reducing blood sugar.
Method for preparing and the application in functional food and medicine thereof of from fresh chickpea bean embryo, bean sprout section or exsiccant chick-pea embryo powder, bean sprout powder, extracting the blood sugar lowering effective ingredient of the present invention comprises following processing step:
The preparation of chick-pea embryo, bean sprout
With the bean material after selected, after washing is clean, put into container, require to have elution device above the container, bottom sides is the 5cm-6cm water hole passage of holding concurrently along reserving the aperture.Add an amount of water again and soak, be beneficial to its germination.Usually soak 2h-3h, can guarantee that the raw material beans can suction moisture.To after soaking, suction the raw material beans of moisture, every separated 5h-6h trickle is 1 time under the room temperature.The bean sprout is grown to the 6cm-8cm long time and is got final product, no longer than 10cm;
The preparation of chick-pea embryo, bean sprout ethanol extract
The plumule that chickpea is fresh, bean sprout section (about 2 millimeters of every segment length) or exsiccant plumule, bean sprout (25 order) place round-bottomed flask; Adding concentration is the alcohol of 10-100%, with ultrasound wave or merceration or heating and refluxing extraction 1-15 time, and each 1 hour; Filter paper filtering; Merging filtrate, being evaporated to does not have the alcohol flavor, obtains alcohol extraction and carries thing;
The preparation of chick-pea embryo, bean sprout water extract
Fresh plumule, bean sprout section (about 2 millimeters of every segment length) or exsiccant chick-pea embryo, bean sprout (25 order) are placed round-bottomed flask; Add pure water; With ultrasound wave or merceration or heating and refluxing extraction 1-15 time, each 2 hours, use gauze, filter paper filtering respectively; Merging filtrate, concentrating under reduced pressure obtains water extract;
Chick-pea embryo, bean sprout valid part comprise: chemical compounds such as total saponins class, osajin.
Chick-pea embryo, bean sprout valid part are as the hypoglycemic application of preparation.Can use chick-pea embryo, bean sprout water extract raw material as pharmaceutical preparation and food; Reduction for processing cost; The raising of end product quality; The stable of curative effect will play positive meaning, also help the basic research of deep processing chickpea bean sprout effective substance simultaneously, the safety of expanding this medicine.
The specific embodiment
Embodiment 1 (ethanol extract)
With conventional method chickpea is germinateed;
With the chickpea raw material after selected, after washing is clean, put into container, require to have elution device above the container; Bottom sides adds entry again and soaks along reserving the aperture for the 5cm water hole passage of holding concurrently, and is beneficial to its germination; Usually soak 2h, can guarantee that the raw material beans can suction moisture, will after soaking, suction the raw material beans of moisture; Every separated 5h trickle is 1 time under the room temperature, and the bean sprout is grown to the 6cm long time and got final product, no longer than 10cm;
The preparation of chick-pea embryo, bean sprout ethanol extract:
With exsiccant chick-pea embryo, bean sprout powder 25 orders that sieve, each 5 gram places round-bottomed flask, adds 2 times of volumetric concentrations and be 10% methanol; Get 15 times with ultrasound wave, each 1 hour, filter paper filtering; Merging filtrate; Ultrasound wave control temperature is at 20 ℃, and being evaporated to does not have the alcohol flavor, obtains ethanol extract effective site.
Embodiment 2 (water extract)
With conventional method chickpea is germinateed;
With the chickpea raw material after selected, after washing is clean, put into container, require to have elution device above the container; Bottom sides adds entry again and soaks along reserving the aperture for the 6cm water hole passage of holding concurrently, and is beneficial to its germination; Usually soak 3h, can guarantee that the raw material beans can suction moisture, will after soaking, suction the raw material beans of moisture; Every separated 6h trickle is 1 time under the room temperature, and the bean sprout is grown to the 7cm long time and got final product, no longer than 10cm;
The preparation of chick-pea embryo and bean sprout water extract:
With exsiccant chick-pea embryo powder and bean sprout powder 25 orders that sieve, each 5 gram places round-bottomed flask, adds the pure water of 2 times of volumes; With ultrasonic extraction 15 times; Each 2 hours, use gauze, filter paper filtering respectively, merging filtrate; Ultrasound wave control temperature is at 20 ℃, and concentrating under reduced pressure obtains water extract effective site.
Embodiment 1 and 2 effective sites comprise: chemical compounds such as total saponins class, osajin, and as the purposes of the hypoglycemic medicine of preparation treatment diabetes or as the purposes for preparing function of blood sugar reduction Sexual health promotion food.
Screening technique is:
With right-nitrobenzophenone disodium hydrogen phosphate (pNPP) as substrate; Being contrast at the positive drug vanadic acid sodium; Utilize ELIASA to carry out the high flux screening of PTP1B enzyme inhibitor, produce the activity that color reaction is measured PTP1B according to the phosphate group of PTP1B hydrolysis pNPP.The enzyme reaction system is formed as follows: buffer (50mM HEPES, pH7.3,100mM sodium chloride, 0.1% bovine serum albumin and 1mM dithiothreitol dithio), PTP1B fusion rotein, pNPP, the special inhibitor vanadate of PTP1B (100 μ g/mL).Place 30rnin at 37 ℃ behind the reaction system mixing, add 1M sodium hydroxide cessation reaction, put the absorption value of measuring on the colour comparatour under the 405 wavelength conditions (A), measure and calculate enzymatic activity after the result deducts background values.
The result:
Chick-pea embryo powder water extract group, ethanol extract group, bean sprout powder water extract group, ethanol extract group be the activity of CKIs tyrosine phosphatase 1B significantly all, blood sugar regulation, and the result is following:
| Blank | Bean sprout powder water extract | Bean sprout powder ethanol extract | The germ flour water extract | The germ flour ethanol extract | |
| Concentration (μ g/mL) A (0min) A (30min)Suppression ratio (%) | 0.061 0.64 | 100 0.08 0.092 97.93 | 75 0.055 0.06 99.13 | 100 0.054 0.07 96.50 | 100 0.052 0.06 98.62 |
Chick-pea embryo and bean sprout valid part also have blood sugar reducing function to alloxan hyperglycemia model mice.
Embodiment 3 (ethanol extract)
With conventional method chickpea is germinateed;
With the chickpea raw material after selected, after washing is clean, put into container, require to have elution device above the container; Bottom sides adds entry again and soaks along reserving the aperture for the 5.5cm water hole passage of holding concurrently, and is beneficial to its germination; Usually soak 2.5h, can guarantee that the raw material beans can suction moisture, will after soaking, suction the raw material beans of moisture; Every separated 5.5h trickle is 1 time under the room temperature, and the bean sprout is grown to the 8cm long time and got final product, no longer than 10cm;
The preparation of chick-pea embryo bean sprout ethanol extract
Chick-pea embryo and bean sprout are cut into chunks, about 2 millimeters of every segment length, each 5 gram places round-bottomed flask; Add 5 times of volumetric concentrations and be 50% ethanol, with heating and refluxing extraction 5 times, each 2 hours; Filter paper filtering, merging filtrate, reflux temperature control temperature is at 80 ℃; Being evaporated to does not have the alcohol flavor, obtains ethanol extract effective site.
Embodiment 4 (water extract)
With conventional method chickpea is germinateed;
With the chickpea raw material after selected, after washing is clean, put into container, require to have elution device above the container; Bottom sides adds entry again and soaks along reserving the aperture for the 5.5cm water hole passage of holding concurrently, and is beneficial to its germination; Usually soak 2.5h, can guarantee that the raw material beans can suction moisture, will after soaking, suction the raw material beans of moisture; Every separated 5.5h trickle is 1 time under the room temperature, and the bean sprout is grown to the 8cm long time and got final product, no longer than 10cm;
The preparation of chick-pea embryo, bean sprout water extract
Chick-pea embryo and bean sprout are cut into chunks, about 2 millimeters of every segment length, each 5 gram places round-bottomed flask; The pure water that adds 5 times of volumes, with heating and refluxing extraction 5 times, each 3 hours; Use gauze, filter paper filtering respectively; Merging filtrate, reflux temperature are controlled at 60 ℃, and concentrating under reduced pressure obtains water extract effective site.
Embodiment 3 and 4 effective ingredient comprise: total saponins class, isoflavonoid are as the purposes of the hypoglycemic medicine of preparation treatment diabetes or as the purposes for preparing function of blood sugar reduction Sexual health promotion food.
Screening technique is identical with screening technique among the embodiment 1, its result:
Chick-pea embryo water extract group, ethanol extract group, bean sprout water extract group, ethanol extract group be the activity of CKIs tyrosine phosphatase 1B significantly all, blood sugar regulation, and the result is following:
| Blank | The bean sprout water extract | The bean sprout ethanol extract | The plumule water extract | The plumule ethanol extract | |
| Concentration (μ g/mL) A (0min) A (30min)Suppression ratio (%) | ?0.061 ?0.64 | 100 0.078 0.086 98.62 | ?100 ?0.065 ?0.072 ?98.79 | ?50 ?0.075 ?0.098 ?96.03 | ?100 ?0.06 ?0.073 ?97.75 |
Chick-pea embryo and bean sprout effective ingredient also have blood sugar reducing function to alloxan hyperglycemia model mice.
Embodiment 5 (ethanol extract)
With conventional method chickpea is germinateed;
With the chickpea raw material after selected, after washing is clean, put into container, require to have elution device above the container, bottom sides is the 6cm water hole passage of holding concurrently along reserving the aperture.Add entry again and soak, be beneficial to its germination, soak 2h usually, can guarantee that the raw material beans can suction moisture, will after soaking, suction the raw material beans of moisture, every separated 5.5h trickle is 1 time under the room temperature, and the bean sprout is grown to the 7cm long time and got final product, no longer than 10cm;
The preparation of chick-pea embryo, bean sprout ethanol extract
Each 5 gram of exsiccant chick-pea embryo powder and bean sprout powder (25 order) are placed round-bottomed flask, adds 8 times of volumetric concentrations and be 70% isopropyl alcohol, with merceration extraction 8 times; Each 3 hours, filter paper filtering, merging filtrate; Being evaporated to does not have the alcohol flavor, obtains ethanol extract effective site.
Embodiment 6 (water extract)
With conventional method chickpea is germinateed;
With the chickpea raw material after selected, after washing is clean, put into container, require to have elution device above the container, bottom sides is the 6cm water hole passage of holding concurrently along reserving the aperture.Add entry again and soak, be beneficial to its germination, soak 2h usually, can guarantee that the raw material beans can suction moisture, will after soaking, suction the raw material beans of moisture, every separated 5.5h trickle is 1 time under the room temperature, and the bean sprout is grown to the 7cm long time and got final product, no longer than 10cm;
The preparation of chick-pea embryo, bean sprout water extract
Exsiccant chick-pea embryo powder, each 5 gram of bean sprout powder (25 order) are placed round-bottomed flask, add the pure water of 8 times of volumes, extract 8 times with merceration, each 4 hours, use gauze, filter paper filtering respectively, merging filtrate, concentrating under reduced pressure obtain water extract effective site.
Embodiment 5 and 6 effective ingredient comprise: total saponins class, isoflavonoid are as the hypoglycemic purposes of medicine of preparation treatment diabetes or as the purposes for preparing function of blood sugar reduction Sexual health promotion food.
Screening technique is identical with screening technique among the embodiment 1, its result:
Chick-pea embryo water extract group, ethanol extract group, bean sprout water extract group, ethanol extract group be the activity of CKIs tyrosine phosphatase 1B significantly all, blood sugar regulation, and the result is following:
| Blank | Bean sprout powder water extract | Bean sprout powder ethanol extract | The germ flour water extract | The germ flour ethanol extract | |
| Concentration (μ g/mL) A (0min) A (30min) | 0.061 0.64 | 100 0.072 0.079 | 100 0.054 0.063 | 50 0.073 0.088 | 250 0.059 0.071 |
| Suppression ratio (%) | 98.79 | ?98.4 | ?97.41 | 97.92 |
Chick-pea embryo and bean sprout effective ingredient also have blood sugar reducing function to alloxan hyperglycemia model mice.
Embodiment 7 (ethanol extract)
With conventional method chickpea is germinateed:
With the chickpea raw material after selected, after washing is clean, put into container, require to have elution device above the container, bottom sides is the 5cm water hole passage of holding concurrently along reserving the aperture.Add entry again and soak, be beneficial to its germination, soak 3h usually, can guarantee that the raw material beans can suction moisture, will after soaking, suction the raw material beans of moisture, every separated 6h trickle is 1 time under the room temperature, and the bean sprout is grown to the 8cm long time and got final product, no longer than 10cm;
The preparation of chick-pea embryo and bean sprout ethanol extract:
Chick-pea embryo and bean sprout are cut into chunks, about 2 millimeters of every segment length, each 5 gram places round-bottomed flask; Add 10 times of volumetric concentrations and be 100% butanols, with ultrasonic extraction 10 times, each 1.5 hours; Filter paper filtering, merging filtrate, ultrasound wave control temperature is at 35 ℃; Being evaporated to does not have the alcohol flavor, obtains ethanol extract effective site.
Embodiment 8 (water extract)
With conventional method chickpea is germinateed:
With the chickpea raw material after selected, after washing is clean, put into container, require to have elution device above the container, bottom sides is the 5cm water hole passage of holding concurrently along reserving the aperture.Add entry again and soak, be beneficial to its germination, soak 3h usually, can guarantee that the raw material beans can suction moisture, will after soaking, suction the raw material beans of moisture, every separated 6h trickle is 1 time under the room temperature, and the bean sprout is grown to the 8cm long time and got final product, no longer than 10cm;
The preparation of chick-pea embryo, bean sprout water extract:
Chick-pea embryo and bean sprout are cut into chunks, about 2 millimeters of every segment length, each 5 gram places round-bottomed flask; The pure water that adds 8 times of volumes, with ultrasonic extraction 10 times, each 2 hours; Use gauze, filter paper filtering respectively; Merging filtrate, ultrasound wave control temperature is at 35 ℃, and concentrating under reduced pressure obtains water extract effective site.
Embodiment 7 and 8 effective ingredient comprise: chemical compounds such as total saponins class, osajin, and as the purposes of the hypoglycemic medicine of preparation treatment diabetes or as the purposes for preparing function of blood sugar reduction Sexual health promotion food.
Screening technique is identical with screening technique among the embodiment 1, its result:
Chick-pea embryo water extract group, ethanol extract group, bean sprout water extract group, ethanol extract group be the activity of CKIs tyrosine phosphatase 1B significantly all, blood sugar regulation, and the result is following:
| Blank | The bean sprout water extract | The bean sprout ethanol extract | The plumule water extract | The plumule ethanol extract | |
| Concentration (μ g/mL) A (0min) A (30min)Suppression ratio (%) | 0.061 0.64 | 100 0.078 0.091 97.90 | 75 0.057 0.062 99.23 | 100 0.055 0.071 96.51 | 100 0.053 0.060 98.72 |
Chick-pea embryo and bean sprout effective ingredient also have blood sugar reducing function to alloxan hyperglycemia model mice.
Embodiment 9 (ethanol extract)
With conventional method chickpea is germinateed;
With the chickpea raw material after selected, after washing is clean, put into container, require to have elution device above the container; Bottom sides adds entry again and soaks along reserving the aperture for the 6cm water hole passage of holding concurrently, and is beneficial to its germination; Usually soak 3h, can guarantee that the raw material beans can suction moisture, will after soaking, suction the raw material beans of moisture; Every separated 5h trickle is 1 time under the room temperature, and the bean sprout is grown to the 6cm long time and got final product, no longer than 10cm;
The preparation of chick-pea embryo, bean sprout ethanol extract
Each 5 gram of exsiccant chick-pea embryo powder, bean sprout powder (25 order) are placed round-bottomed flask, add 4 times of volumetric concentrations and be 40% ethanol, with heating and refluxing extraction 3 times; Each 3 hours; Filter paper filtering, merging filtrate, reflux temperature are controlled at 75 ℃; Being evaporated to does not have the alcohol flavor, obtains ethanol extract effective site.
Embodiment 10 (water extract)
With conventional method chickpea is germinateed;
With the chickpea raw material after selected, after washing is clean, put into container, require to have elution device above the container; Bottom sides adds entry again and soaks along reserving the aperture for the 6cm water hole passage of holding concurrently, and is beneficial to its germination; Usually soak 3h, can guarantee that the raw material beans can suction moisture, will after soaking, suction the raw material beans of moisture; Every separated 5h trickle is 1 time under the room temperature, and the bean sprout is grown to the 6cm long time and got final product, no longer than 10cm;
The preparation of chick-pea embryo and bean sprout water extract
Chick-pea embryo and bean sprout are cut into chunks, about 2 millimeters of every segment length, each 5 gram places round-bottomed flask; The pure water that adds 4 times of volumes, with heating and refluxing extraction 3 times, each 4 hours; Use gauze, filter paper filtering respectively; Merging filtrate, reflux temperature are controlled at 96 ℃, and concentrating under reduced pressure obtains water extract effective site.
Embodiment 9 and 10 effective ingredient comprise: chemical compounds such as total saponins class, osajin, and as the preparation treatment hypoglycemic medicinal usage of diabetes or as the purposes for preparing function of blood sugar reduction Sexual health promotion food.
Screening technique is identical with screening technique among the embodiment 1, its result:
Chick-pea embryo water extract group, ethanol extract group, bean sprout water extract group, ethanol extract group be the activity of CKIs tyrosine phosphatase 1B significantly all, blood sugar regulation, and the result is following:
| Blank | The bean sprout water extract | The bean sprout ethanol extract | The plumule water extract | The plumule ethanol extract | |
| Concentration (μ g/mL) A (0min) A (30min)Suppression ratio (%) | 0.061 0.64 | 100 0.076 0.084 98.53 | 100 0.064 0.073 98.59 | 50 0.072 0.095 96.13 | 100 0.062 0.071 97.55 |
Chick-pea embryo and bean sprout effective ingredient also have blood sugar reducing function to alloxan hyperglycemia model mice.
Embodiment 11 (ethanol extract)
With conventional method chickpea is germinateed;
With the chickpea raw material after selected, after washing is clean, put into container, require to have elution device above the container; Bottom sides adds entry again and soaks along reserving the aperture for the 5cm water hole passage of holding concurrently, and is beneficial to its germination; Usually soak 2.5h, can guarantee that the raw material beans can suction moisture, will after soaking, suction the raw material beans of moisture; Every separated 5.5h trickle is 1 time under the room temperature, and the bean sprout is grown to the 8cm long time and got final product, no longer than 10cm;
The preparation of chick-pea embryo, bean sprout ethanol extract
Cut into chunks in fresh chick-pea embryo section, bean sprout, about 2 millimeters of every segment length, each 5 gram places round-bottomed flask; Add 5 times of volumetric concentrations and be 60% isopropyl alcohol, with ultrasonic extraction 3 times, each 1.5 hours; Filter paper filtering, merging filtrate, ultrasound wave control temperature is at 30 ℃; Being evaporated to does not have the alcohol flavor, obtains ethanol extract effective site.
Embodiment 12 (water extract)
With conventional method chickpea is germinateed;
With the chickpea raw material after selected, after washing is clean, put into container, require to have elution device above the container; Bottom sides adds entry again and soaks along reserving the aperture for the 5cm water hole passage of holding concurrently, and is beneficial to its germination; Usually soak 2.5h, can guarantee that the raw material beans can suction moisture, will after soaking, suction the raw material beans of moisture; Every separated 5.5h trickle is 1 time under the room temperature, and the bean sprout is grown to the 8cm long time and got final product, no longer than 10cm;
The preparation of chick-pea embryo, bean sprout water extract
Cut into chunks in fresh chick-pea embryo section, bean sprout, about 2 millimeters of every segment length, each 5 gram places round-bottomed flask; The pure water that adds 5 times of volumes, with ultrasonic extraction 15 times, each 2 hours; Use gauze, filter paper filtering respectively; Merging filtrate, ultrasound wave control temperature is at 40 ℃, and concentrating under reduced pressure obtains water extract effective site.
Embodiment 11 and 12 effective ingredient comprise: chemical compounds such as total saponins class, osajin are as the preparation treatment hypoglycemic medicinal usage of diabetes or as the purposes for preparing function of blood sugar reduction Sexual health promotion food.
Screening technique is identical with screening technique among the embodiment 1, its result:
Chick-pea embryo water extract group, ethanol extract group, bean sprout water extract group, ethanol extract group be the activity of CKIs tyrosine phosphatase 1B significantly all, blood sugar regulation, and the result is following:
| Blank | The bean sprout water extract | The bean sprout ethanol extract | The plumule water extract | The plumule ethanol extract | |
| Concentration (μ g/mL) A (0min) A (30min)Suppression ratio (%) | 0.061 0.64 | 100 0.065 0.077 98.41 | 100 0.061 0.072 98.51 | 50 0.071 0.079 98.65 | 100 0.053 0.066 98.54 |
Chick-pea embryo and bean sprout effective ingredient also have blood sugar reducing function to alloxan hyperglycemia model mice.
Embodiment 13 (ethanol extract)
With conventional method chickpea is germinateed;
With the chickpea raw material after selected, after washing is clean, put into container, require to have elution device above the container; Bottom sides adds entry again and soaks along reserving the aperture for the 5cm water hole passage of holding concurrently, and is beneficial to its germination; Usually soak 3h, can guarantee that the raw material beans can suction moisture, will after soaking, suction the raw material beans of moisture; Every separated 6h trickle is 1 time under the room temperature, and the bean sprout is grown to the 8cm long time and got final product, no longer than 10cm;
The preparation of chick-pea embryo and bean sprout ethanol extract
Cut into chunks in fresh chick-pea embryo section, bean sprout, about 2 millimeters of every segment length, each 5 gram places round-bottomed flask; Add 7 times of volumetric concentrations and be 90% methanol, with ultrasonic extraction 10 times, each 1 hour; Filter paper filtering, merging filtrate, the ultrasound wave temperature is controlled at 25 ℃; Being evaporated to does not have the alcohol flavor, obtains ethanol extract effective site.
Embodiment 14 (water extract)
With conventional method chickpea is germinateed;
With the chickpea raw material after selected, after washing is clean, put into container, require to have elution device above the container; Bottom sides adds entry again and soaks along reserving the aperture for the 5cm water hole passage of holding concurrently, and is beneficial to its germination; Usually soak 3h, can guarantee that the raw material beans can suction moisture, will after soaking, suction the raw material beans of moisture; Every separated 6h trickle is 1 time under the room temperature, and the bean sprout is grown to the 8cm long time and got final product, no longer than 10cm;
The preparation of chick-pea embryo, bean sprout water extract
Cut into chunks in fresh chick-pea embryo section, bean sprout, about 2 millimeters of every segment length, each 5 gram places round-bottomed flask; The pure water that adds 10 times of volumes, with ultrasonic extraction 5 times, each 2 hours; Use gauze, filter paper filtering respectively; Merging filtrate, ultrasound wave temperature are controlled at 33 ℃, and concentrating under reduced pressure obtains water extract effective site.
Embodiment 13 and 14 effective sites comprise: total saponins class, isoflavonoid, and as the preparation treatment hypoglycemic medicinal usage of diabetes or as the purposes for preparing function of blood sugar reduction Sexual health promotion food.
Screening technique is identical with screening technique among the embodiment 1, its result:
Chick-pea embryo water extract group, ethanol extract group, bean sprout water extract group, ethanol extract group be the activity of CKIs tyrosine phosphatase 1B significantly all, blood sugar regulation, and the result is following:
| Blank | The bean sprout water extract | The bean sprout ethanol extract | The plumule water extract | The plumule ethanol extract | |
| Concentration (μ g/mL) A (0min) A (30min)Suppression ratio (%) | 0.061 0.64 | 100 0.057 0.066 98.04 | 100 0.061 0.069 98.21 | 50 0.067 0.078 98.59 | 100 0.050 0.061 98.24 |
Chick-pea embryo and bean sprout effective ingredient also have blood sugar reducing function to alloxan hyperglycemia model mice.
Claims (8)
1. the plumule of a chickpea that adopts solvent extraction method preparation and the effective site of bean sprout is characterized in that following these steps to carrying out:
A, chickpea is germinateed with conventional method;
B, be methanol or ethanol or isopropyl alcohol or the butanols of 10-100% with the concentration that adds 2-10 times of volume in the germ flour of the plumule of fresh chickpea and bean sprout or exsiccant chickpea and the bean sprout powder;
C, with ultrasound wave or merceration or heating and refluxing extraction 1-15 time, each 1-3 hour, wherein the ultrasonic extraction temperature was 20-40 ℃, the reflux, extract, temperature is 60-80 ℃, filter, merging filtrate, being evaporated to does not have the alcohol flavor, obtains ethanol extract effective site;
D or with the pure water that adds 2-10 times of volume in the germ flour of the plumule of fresh chickpea and bean sprout or exsiccant chickpea and the bean sprout powder;
E, with ultrasound wave or merceration or heating and refluxing extraction 1-15 time, each 2-4 hour, wherein the ultrasonic extraction temperature was 20-60 ℃, the reflux, extract, temperature is 60-100 ℃, filters, merging filtrate, concentrating under reduced pressure obtain water extract effective site.
2. the effective site of the plumule of chickpea according to claim 1 and bean sprout is characterized in that effective site is total saponins class and osajin.
3. the effective site of the plumule of chickpea according to claim 1 and bean sprout is characterized in that effective site is prepared as functional food or processes capsule or tablet or oral liquid or electuary or injection by conventional pharmaceutical methods by conventional method; Or with effective site with in, the Western medicine compatibility processes capsule or tablet or oral liquid or electuary or injection.
4. the method for preparing of the effective site of the plumule of a chickpea and bean sprout is characterized in that it is alcohol extracting method or water extraction preparation that this effective site adopts solvent extraction method, and concrete operations follow these steps to carry out:
A, chickpea is germinateed with conventional method;
B, with the methanol or ethanol or isopropyl alcohol or the butanols that add 2-10 times of volume in the germ flour of the plumule of fresh chickpea and bean sprout or exsiccant chickpea and the bean sprout powder, its concentration is 10-100%;
C, with ultrasound wave or merceration or heating and refluxing extraction 1-15 time, each 1-3 hour, wherein the ultrasonic extraction temperature was 20-40 ℃; The reflux, extract, temperature is 60-80 ℃, filters merging filtrate; Being evaporated to does not have the alcohol flavor, obtains ethanol extract effective site total saponins class, osajin;
D or with the pure water that adds 2-10 times of volume in the germ flour of the plumule of fresh chickpea and bean sprout or exsiccant chickpea and the bean sprout powder;
E, with ultrasound wave or merceration or heating and refluxing extraction 1-15 time, each 2-4 hour, wherein the ultrasonic extraction temperature was 20-60 ℃; The reflux, extract, temperature is 60-100 ℃; Filter, merging filtrate, concentrating under reduced pressure obtain water extract effective site total saponins class, osajin.
5. method for preparing according to claim 4 is characterized in that the determining alcohol among the step b is 60-70%.
6. method for preparing according to claim 4 is characterized in that step c alcohol ultrasonic extraction temperature is 30-35 ℃, and the reflux, extract, temperature is 70-80 ℃.
7. method for preparing according to claim 4 is characterized in that the ultrasonic extraction temperature is 30-40 ℃ among the step e, and the reflux, extract, temperature is 80-96 ℃.
8. the effective site of the plumule of the chickpea of claim 1 and bean sprout is in the hypoglycemic drug of preparation treatment diabetes or the purposes in the food with function of reducing blood sugar.
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| CN101991635B (en) * | 2010-10-28 | 2011-09-07 | 中国科学院新疆理化技术研究所 | Preparation method of chickpea total saponin |
| CN102068486B (en) * | 2010-12-30 | 2012-04-18 | 中国科学院新疆理化技术研究所 | Effective fractions of cicer arietinum linn bean sprout and preparation method and application thereof |
| CN106551211A (en) * | 2015-09-30 | 2017-04-05 | 昌吉州丝路弘毅农业科技有限公司 | A kind of chickpea bean sprout plant beverage and preparation method thereof |
| CN107232600A (en) * | 2016-03-25 | 2017-10-10 | 广州宏韵医药科技股份有限公司 | The preparation method and application of black soya bean sprout extract |
| CN106723043A (en) * | 2017-01-04 | 2017-05-31 | 郭徽 | A kind of food compositions of three high drop |
| CN108576818B (en) * | 2018-04-20 | 2021-07-30 | 新疆天山小哥农业发展有限公司 | Chickpea germ powder and preparation method thereof |
| CN114053325A (en) * | 2021-12-09 | 2022-02-18 | 广州中医药大学(广州中医药研究院) | Chickpea effective part and preparation method and application thereof |
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