CN103444604B - Analysis of Cromileptes altivelis small size pond offspring seed cultivation method - Google Patents

Analysis of Cromileptes altivelis small size pond offspring seed cultivation method Download PDF

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CN103444604B
CN103444604B CN201310366818.0A CN201310366818A CN103444604B CN 103444604 B CN103444604 B CN 103444604B CN 201310366818 A CN201310366818 A CN 201310366818A CN 103444604 B CN103444604 B CN 103444604B
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water
pond
day
prelarva
parent population
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CN103444604A (en
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严峰
邱勇
吴廷昌
陈国华
骆剑
吴小易
王珺
杨薇
何佳
李丽
郭孝伟
冯治中
詹绍芬
余庆
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Hainan University
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Hainan University
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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Abstract

The invention discloses a kind of analysis of Cromileptes altivelis small size pond offspring seed cultivation method, it is originated by parent population and selects; The training of parent population and cultivation; The artificial repening of parent population with the step such as to hasten parturition, cultivate and be applicable to selling and cultivation nursery.It is low that method of the present invention has cost, workable, and survival rate of seedling is higher, the feature of administration measure.

Description

Analysis of Cromileptes altivelis small size pond offspring seed cultivation method
Technical field
The present invention relates to a kind of analysis of Cromileptes altivelis small size pond rearing of fingerling, belong to seedling growing process field.
Background technology
Analysis of Cromileptes altivelis is again mouse spot (Cromileptesaltivelis) because head proboscis point, and shape exactly likes mouse and gains the name, and belongs to section, Epinephelinae, and analysis of Cromileptes altivelis belongs to.Analysis of Cromileptes altivelis is the economic edible fishes of senior marine products, and price is high, deeply welcomes by the masses.In recent years analysis of Cromileptes altivelis market sold, be all sub-national southeast usually, though South China Sea has the record of this kind of fish, quantity is seldom the fingerling having much high economic worth in ablen.Analysis of Cromileptes altivelis because its growth rate is fast, and can be stood in the existence of space-constrained waters, and cultivation development potentiality is quite large.At present, most analysis of Cromileptes altivelis cultivation nursery is from the marine wild fry fished for, and the output of wild seedling has been unable to supply the demand in market, needs the technology of a set of mature and stable artificial culture analysis of Cromileptes altivelis seedling.
Summary of the invention
The object of this invention is to provide a kind of analysis of Cromileptes altivelis small size pond rearing of fingerling, for analysis of Cromileptes altivelis fry rearing, solve the problem of wild analysis of Cromileptes altivelis seedling deficiency on market.
The technical scheme that the present invention adopts for its technical problem of solution is:
A kind of analysis of Cromileptes altivelis small size pond rearing of fingerling, adopts following method step:
1. parent population is originated and selects
Choose the mouse spot that the body weight of catching in sea, natural marine site is 1 ~ 2kg, the individuality healthy, nothing is hindered, body surface is complete, bright in colour, biological property is obvious, vigor is good is as parent population.
2. the admonitory talk to subordinates of parent population and cultivation
Select stormy waves little, water quality is good, pollutes little sea area, annual water temperature 12 ~ 28 DEG C, and salinity 24 ~ 30, puts the net cage in specification 3x3x3m in a suitable place to breed, and breeding density is 4 ~ 8kg/m.Feed every day and raise fresh lower trash fish meat (high containing protein, the fish that fat is low are the most applicable), throwing the amount of raising is all plant fish body weight 5 ~ 10%.In addition, for accelerating gonadal maturation, VitaminE (10-20mg/kg body weight, weekly) can in bait, be added.For improving muscle power and the health of kind of fish, can add VitaminC and B-complex (50-80mg/kg body weight, once every two weeks).
3. parent population artificial repening with hasten parturition
Select the net cage of hastening parturition that belly is comparatively large, parent population that is that appear sexual gland profile puts into dark.Male and female parent population is in the collocation of 1:1 ratio, and injection oxytocic hormone, dosage is HCG400 ~ 500 international unit and LRH-A330 ~ 50ug/kg fish body weight, and two kinds used in combination, uses 0.6% normal saline dilution, make suspension.Injection volume is 1.5-2ml, and pectoral fin base portion is injected, and milter injected dose is 1/2 of raun.Soft to some bellies, after the obvious parent population of sexual gland profile puts into net cage of hastening parturition, the method that ecological current stimulate can be adopted.
4. fertilized egg process
The naturally fertilized egg originated in pond is collected with 80 order-100 mesh sieve thin,tough silk trawlnets.The fertilized egg of collecting, through repeatedly washing ovum, after removing the bad ovum and impurity sunk to the bottom, is placed in 0.5t and bores end black hatching pail inflation hatching.Prelarva is stopped the supple of gas or steam after hatching, and opens the dirt of boring bottom under sedimentation got rid of by bottom valve and also thoroughly changes water, be then with by prelarva water to move in nursery pond and cultivate.
5. seed rearing
Nursery pond condition: indoor water mud sump, square, specification is 5m × 5m × 1m; The discharge outlet of arranging under being provided with overflow and Chi Dipaiwukou; 25-30 gas stone inflation is established in full pond.11:00-15:00 nursery pond water surface intensity of illumination controls at 3000-5200lx.EM bacterium: commercially available east bacterium king 10L, clear water 20L, brown sugar 2kg, mix in 50L polyethylene drum, seals for subsequent use.Prawn slice: the senior prawn slice of commercially available golden shrimp board.Biological feed: with wheel animalcule, the copepoda of pond rearing.
5.1 nursery pond water quality managements
Nursery pond with bleaching powder sterilization, rinses extremely without chlorine residue before use.Add seawater dark to 80cm.Nursery pond water inlet same day, select every day 8 and 15 select left and right and to splash in pond for 2 times ready EM bacterium and prawn slice.Throw in prelarva: training water is to 4-6 days.
5.2 seedling fostering methods
Carefully nursery pond is rendered to after hatching prelarva; Under prelarva after the 3h of pond, it is 1.1 ten thousand-2 ten thousand tails/m that sampling measures density 3.Bait feeding prelarva hatches the 2nd day, inoculating rotifer in nursery pond, keeps seedling water wheel animalcule density 15-20/mL.14th day starts, continues to throw something and feed outside wheel animalcule, throws something and feeds on a small quantity through the copepod larva of 80 order silk cover filterings.20-25 days, based on copepoda of throwing something and feeding, keeps seedling water copepoda density to be 5-10/mL.Under prelarva behind pond: first week, do not change water; Second week, add water every day 1-2cm in pond; 3rd week every day changed water 3-8cm; After 4th week, according to nursery water quality condition, change water 20-30cm every day.Approximately continue 30 days-50 days, can cultivate and be applicable to selling and cultivation nursery
The invention has the beneficial effects as follows: after carrying out 30 days according to seedling-cultivating method of the present invention, survival rate of seedling is 2.70 ~ 3.90%, and survival rate is higher than other conventional fry methods.It is low, workable that this analysis of Cromileptes altivelis small size pond offspring seed cultivation method has cost, and survival rate of seedling is higher, the feature of administration measure.
Embodiment
Below in conjunction with specific embodiment, the present invention is further described:
Embodiment:
1. parent population is originated and selects
Buying the body weight of catching in sea, natural marine site in Taiwan is about 1.5kg mouse spot, and the individuality healthy, nothing is hindered, body surface is complete, bright in colour, biological property is obvious, vigor is good is as parent population.
2. the admonitory talk to subordinates of parent population and cultivation
Arrange this sheet sea area stormy waves in the fishing of Lingshui, Hainan Qing Li aquatic products company little, water quality is good, pollutes little sea area, annual water temperature 14 ~ 30 DEG C, and salinity 25 ~ 30, puts the net cage in specification 3x3x3m in a suitable place to breed, and breeding density is 6kg/m.Feed every day and raise fresh lower trash fish meat (high containing protein, the fish that fat is low are the most applicable), throwing the amount of raising is all plant fish body weight 5%.In addition VitaminE (10mg/kg body weight, weekly) can be added in bait.For improving muscle power and the health of kind of fish, add VitaminC and B-complex (50mg/kg body weight, once every two weeks).
3. parent population artificial repening with hasten parturition
Select the net cage of hastening parturition that belly is comparatively large, parent population that is that appear sexual gland profile puts into dark.Male and female parent population is in 1:1 ratio collocation (more than 100 tails of hastening parturition) at every turn, and injection oxytocic hormone, dosage is that HCG400 international unit+LRH-A3 is about 40ug/kg fish body weight, and two kinds used in combination, uses 0.6% normal saline dilution, make suspension.Injection volume is 1.5, and pectoral fin base portion is injected, and milter injected dose is 1/2 of raun.
4. fertilized egg process
The naturally fertilized egg originated in pond is collected (parent population that lays eggs can reach 90%) with 80 mesh sieve thin,tough silk trawlnets.The fertilized egg of collecting, through repeatedly washing ovum, after removing the bad ovum and impurity sunk to the bottom, is placed in 0.5t and bores end black hatching pail inflation hatching.Prelarva is stopped the supple of gas or steam after hatching, and opens the dirt of boring bottom under sedimentation got rid of by bottom valve and also thoroughly changes water, be then with by prelarva water to move in nursery pond and cultivate.
5. seed rearing
Nursery pond condition: indoor water mud sump, square, specification is 5m × 5m × 1m; The discharge outlet of arranging under being provided with overflow and Chi Dipaiwukou; 25 gas stone inflations are established in full pond.11:00 ~ 15:00 nursery pond water surface intensity of illumination controls at about 4000lx.EM bacterium: commercially available east bacterium king 10L, clear water 20L, brown sugar 2kg, mix in 50L polyethylene drum, seals for subsequent use.Prawn slice: the senior prawn slice of commercially available golden shrimp board.Biological feed: with wheel animalcule, the copepoda of pond rearing.
5.1 nursery pond water quality managements
Nursery pond with bleaching powder sterilization, rinses extremely without chlorine residue before use.Add seawater dark to 80cm.Nursery pond water inlet same day, select every day 8 and 15 select left and right and to splash in pond for 2 times ready EM bacterium and prawn slice.Throw in prelarva: training water was to the 5th day.
5.2 seedling fostering methods
Hatch prelarva (average every bar parent population can lay eggs 50g) and carefully render to nursery pond afterwards; Under prelarva after the 3h of pond, it is 1.1 ten thousand tails/m3 that sampling measures density.Bait feeding prelarva hatches the 2nd day, inoculating rotifer in nursery pond, keeps seedling water wheel animalcule density 15/mL.14th day starts, continues to throw something and feed outside wheel animalcule, throws something and feeds on a small quantity through the copepod larva of 80 order silk cover filterings.20th day, based on copepoda of throwing something and feeding, seedling water copepoda density is kept to be 5 ~ 10/mL.Under prelarva behind pond: first week, do not change water; Second week, add water every day about 2cm in pond; 3rd week every day changed water about 5cm; After 4th week, according to nursery water quality condition, change water about 20cm every day.Approximately continue 30 days, can cultivate and be applicable to selling and cultivation nursery (emergence rate about 3%, specification 2 ~ 3cm).
The above, be only the specific embodiment of the present invention, but protection scope of the present invention is not limited thereto, and any change of expecting without creative work or replacement, all should be encompassed within protection scope of the present invention.Therefore, the protection domain that protection scope of the present invention should limit with claims is as the criterion.

Claims (2)

1. an analysis of Cromileptes altivelis small size pond offspring seed cultivation method, is characterized in that comprising the steps:
(1) parent population is originated and selects
Choose the mouse spot that the body weight of catching in sea, natural marine site is 1-2kg, select healthy, that nothing wound, body surface are complete individuality as parent population;
(2) domestication of parent population and cultivation
Select little, the annual water temperature of stormy waves 12 ~ 28 DEG C, the sea area of salinity 24 ~ 30, puts in a suitable place to breed in net cage, and breeding density is 4 ~ 8kg/m 3; Every day feeds and raises fresh lower trash fish meat, and throwing the amount of raising is all plant fish body weight 5 ~ 10%;
(3) parent population artificial repening with hasten parturition
Select the net cage of hastening parturition that belly is comparatively large, parent population that is that appear sexual gland profile puts into dark; Injection oxytocic hormone, dosage is HCG400 ~ 500 international unit and LRH-A330 ~ 50ug/kg fish body weight, and two kinds are used in combination, use 0.6% normal saline dilution, make suspension; Injection volume is 1.5-2ml, and pectoral fin base portion is injected, and milter injected dose is about 1/2 of raun;
(4) fertilized egg process
The naturally fertilized egg originated in pond is collected with 80 order-100 mesh sieve thin,tough silk trawlnets; The fertilized egg of collecting, through repeatedly washing ovum, after removing the bad ovum and impurity sunk to the bottom, is placed in 0.5t and bores end black hatching pail inflation hatching; Prelarva is stopped the supple of gas or steam after hatching, and opens the dirt of boring bottom under sedimentation got rid of by bottom valve and also thoroughly changes water, be then with by prelarva water to move in nursery pond and cultivate;
(5) seed rearing
Nursery pond condition: indoor water mud sump, the discharge outlet of arranging under being provided with overflow and the inflation of Chi Dipaiwukou, Quan Chishe gas stone; 11: 00-15: 00 nursery pond water surface intensity of illumination controls at 3000-5200lx; EM bacterium: commercially available east bacterium king 8-12L, clear water 15-25L, brown sugar 1.5-2.5kg, mixing, seals for subsequent use;
Nursery pond water quality management
Nursery pond with bleaching powder sterilization, rinses extremely without chlorine residue before use; Add seawater; Nursery pond water inlet same day, select every day 8 and 15 select left and right and to splash in pond for 2 times ready EM bacterium and prawn slice; Throw in prelarva; Training water is to 4-6 days;
Seedling fostering method
Nursery pond is rendered to after hatching prelarva; Under prelarva after the 3h of pond, it is 1.1 ten thousand-2 ten thousand tails/m that sampling measures density 3; Bait feeding prelarva hatches the 2nd day, inoculating rotifer in nursery pond, keeps seedling water wheel animalcule density 15-20/mL; 14th day starts, continues to throw something and feed outside wheel animalcule, throws something and feeds on a small quantity through the copepod larva of 80 order silk cover filterings; 20-25 days, based on copepoda of throwing something and feeding, keeps seedling water copepoda density to be 5-10/mL; Under prelarva behind pond: first week, do not change water: second week, add water in pond every day changes water 3-8cm 1-2cm: the three week every day: after 4th week, according to nursery water quality condition, change water 20-30cm every day; Continue 30 days-50 days, can cultivate be applicable to sell and cultivation use fry.
2. method according to claim 1, is characterized in that in step (2) being the gonadal maturation of acceleration, presses weekly 10-20mg/kg body weight and add VitaminE once in bait; For improving muscle power and the health of kind of fish, within every two weeks, pressing 50-80mg/kg body weight and adding the B B-complex of VitaminB and C once.
CN201310366818.0A 2013-08-21 2013-08-21 Analysis of Cromileptes altivelis small size pond offspring seed cultivation method Expired - Fee Related CN103444604B (en)

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CN105075940B (en) * 2015-07-14 2018-04-17 海南晨海水产有限公司 A kind of analysis of Cromileptes altivelis and the artificial hybridization breeding method of epinephelus lanceolatus fish
CN105494208A (en) * 2015-12-31 2016-04-20 山东省海洋生物研究院 Sebastes schlegeli artificial squeezing fry breeding method
CN105557595B (en) * 2016-01-20 2018-06-15 中国水产科学研究院长江水产研究所 A kind of breeding method of Qinling Mountains fine-scaled graphite seed
CN105850814A (en) * 2016-05-19 2016-08-17 莱州明波水产有限公司 Industrialized hybrid fry breeding method for chromileptes altivelis and epinephelus lanceolatus
CN111149737B (en) * 2020-02-13 2022-05-24 海南晨海水产有限公司 Outdoor ecological pond artificial breeding method for high-survival-rate lateolabrax japonicus

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