CN103436472B - A kind of compound micro-ecological preparation for improvement of pond water quality - Google Patents
A kind of compound micro-ecological preparation for improvement of pond water quality Download PDFInfo
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Abstract
The invention provides a kind of compound micro-ecological preparation for improvement of pond water quality, the concrete steps of its preparation method: the Rhodopseudomonas palustris in freezing pipe, nitrifier, lactobacillus and yeast saccharomyces cerevisiae are activated respectively in inclined-plane, purifying obtains each slant strains afterwards, for subsequent use; Each slant strains is utilized to prepare corresponding original bacteria liquid; Each original bacteria liquid is inoculated in sterilized fermention medium I by the bacterium amount ratio of 2:2:1:1 and cultivates, obtain the first order seed of compound microecological microbial inoculum; By first order seed by 5% inoculum size be inoculated in sterilized fermention medium II and cultivate to obtain the secondary seed of compound microecological microbial inoculum; Finally by secondary seed by 5% inoculum size be seeded to the finished product that sterilized fermention medium III cultivates to obtain compound microecological microbial inoculum.Adopt compound micro-ecological preparation of the present invention can either improve dissolved oxygen amount in water body, the COD in water body can be reduced again, thus play improvement, optimize the effect of pond water quality.
Description
[technical field]
The present invention relates to a kind of compound micro-ecological preparation of aquaculture field, particularly relate to a kind of compound micro-ecological preparation for improvement of pond water quality.
[background technology]
Since reform and opening-up, the culture fishery of China achieves the development of advancing by leaps and bounds, and becomes unique cultured output in the world and exceedes the fishery big country of fishery output.In the culture fishery of China, pond aquaculture occupies very important status, traditional aquaculture model that it is technical characteristic that existing pond aquaculture has acted on " close, mixed, wheel " mostly.In order to improve the cultured output of unit of water body, covet high yield, often undue increase water nutrition, excessive bait throwing in, fertilising and increasing breeding density, cause the too fast breeding of harmful microorganism in water, harmful algae, and occur that movement and residual bait pollute, destroy the ecology especially micro-ecological environment of aquaculture water, thus the aquaculture organism endangered directly or indirectly in water body, and then the cultivation of high-quality aquatic products kind is made to be subject to the restriction of water quality.Water ecological setting is destroyed, and in pond, the disease of aquatic animal occurs frequent, and fishery is sustained a great loss.In order to prevent or prevent disease, at present in the whole culture-cycle all at use fish medicine, although the use of fish medicine obtains the effect of emergence therapeutic, bring such as resistance, the problem such as accumulation is residual in fishery products and environment more.Therefore, from ecological point, although cultivating pool is a very complicated aquatic ecosystem, no matter how complicated it is, be the problem of an environment after all.The control of cultivating pool water ecological setting has become the key issue of development China pond aquaculture, therefore seems very important to the purification of water quality of aquaculture pond and improvement.
[summary of the invention]
Technical problem to be solved by this invention is to provide a kind of compound micro-ecological preparation for improvement of pond water quality.
The present invention solves the problems of the technologies described above by the following technical programs: a kind of compound micro-ecological preparation for improvement of pond water quality, and the concrete steps of its preparation method are as follows:
(1) former strain inclined plane is cultivated: the Rhodopseudomonas palustris in freezing pipe, nitrifier, lactobacillus and yeast saccharomyces cerevisiae are activated in inclined-plane respectively, be placed in 90mm culture dish afterwards respectively and carry out purifying, obtain Rhodopseudomonas palustris slant strains, nitrifier slant strains, lactobacillus slant strains and yeast saccharomyces cerevisiae slant strains, for subsequent use;
(2) former strain liquid is cultivated: Rhodopseudomonas palustris slant strains be inoculated in sterilized liquid nutrient medium I, within illumination cultivation 5-7 days, obtain Rhodopseudomonas palustris original bacteria liquid afterwards in 28 DEG C-30 DEG C anaerobism tengsten lamps; Nitrifier slant strains be inoculated in sterilized liquid nutrient medium II, then at 25 DEG C-28 DEG C, aerobic cultivation obtains nitrifier original bacteria liquid in 3-5 days; Lactobacillus slant strains be inoculated in sterilized liquid nutrient medium III, then at 37 DEG C, Anaerobic culturel 1-2 days obtains lactobacillus original bacteria liquid; Yeast saccharomyces cerevisiae slant strains be inoculated in sterilized liquid nutrient medium IV, at 28 DEG C-30 DEG C, aerobic cultivation obtains yeast saccharomyces cerevisiae original bacteria liquid in 3 days afterwards;
(3) first order seed is cultivated: Rhodopseudomonas palustris original bacteria liquid, nitrifier original bacteria liquid, lactobacillus original bacteria liquid and yeast saccharomyces cerevisiae original bacteria liquid are inoculated in sterilized fermention medium I by the bacterium amount ratio of 2:2:1:1 and cultivate, particularly: first inoculate nitrifier original bacteria liquid, yeast saccharomyces cerevisiae original bacteria liquid aerobic cultivation 3 days at 28 DEG C-30 DEG C, and then inoculate Rhodopseudomonas palustris original bacteria liquid, lactobacillus original bacteria liquid 37 DEG C of Anaerobic culturel 3 days, obtain the first order seed of compound micro-ecological preparation;
(4) secondary seed is cultivated: by the first order seed of gained by 5% inoculum size be inoculated in sterilized fermention medium II, and at 28 DEG C-30 DEG C first aerobic cultivation 3-5 days Anaerobic culturel 3-5 days again, obtain the secondary seed of compound micro-ecological preparation;
(5) finished product is cultivated: by the secondary seed of gained by 5% inoculum size be seeded in sterilized fermention medium III, and at 28 DEG C-30 DEG C first aerobic cultivation 3-5 days Anaerobic culturel 3-5 days again, obtain the finished product of described compound micro-ecological preparation;
Wherein, the component of liquid nutrient medium I: yeast powder 10 grams, dipotassium hydrogen phosphate 1 gram, 0.5 gram, magnesium sulfate, 20 grams, agar, water 1000ml, PH7.0-7.2; The component of liquid nutrient medium II: SODIUMNITRATE 1 gram, 0.03 gram, magnesium sulfate, manganous sulfate 0.01 gram, dipotassium hydrogen phosphate 0.8 gram, 1 gram, sodium carbonate, calcium superphosphate 0.25 gram, water 1000ml, PH nature; The component of liquid nutrient medium III: peptone 10 grams, extractum carnis 10 grams, yeast powder 5 grams, glucose 5 grams, sodium acetate 5 grams, citric acid diamines 2 grams, tween 80 1ml, dipotassium hydrogen phosphate 2 grams, 0.58 gram, magnesium sulfate, manganous sulfate 0.28 gram, water 1000ml, PH6.5; The component of liquid nutrient medium IV: potato 200 grams, sucrose 20 grams, water 1000ml, PH nature; The component of fermention medium I: tangerine water 5%, yeast powder 0.5%, peptone 1%, ammonium chloride 0.2%, sodium-chlor 0.1%, potassium primary phosphate 0.1%, magnesium sulfate 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water; The component of fermention medium II: tangerine water 5%, yeast powder 0.1%, peptone 0.1%, ammonium chloride 0.2%, sodium-chlor 0.1%, potassium primary phosphate 0.1%, magnesium sulfate 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water; The component of fermention medium III: tangerine water 5%, ammonium chloride 0.2%, sodium-chlor 0.05%, potassium primary phosphate 0.1%, magnesium sulfate 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water.
Further, the concrete operations that in described step (1), Rhodopseudomonas palustris activates in inclined-plane are: be inoculated on sterilized slant medium I with transfering loop by Rhodopseudomonas palustris, afterwards in 28 DEG C-30 DEG C anaerobism tengsten lamp illumination cultivation 5-7 days; The sterilising temp of described slant medium I is 121 DEG C, sterilization time is 15min; And the component of this slant medium I: yeast powder 10 grams, dipotassium hydrogen phosphate 1 gram, 0.5 gram, magnesium sulfate, 20 grams, agar, water 1000ml, PH 7.0-7.2.
Further, the concrete operations that in described step (1), nitrifier activates in inclined-plane are: prepare slant medium II, and sterilizing 20min at slant medium II is placed in 121 DEG C, with transfering loop, nitrifier is inoculated on slant medium II afterwards, then aerobic cultivation 3-5 days at 25 DEG C-28 DEG C; The component of described slant medium II: SODIUMNITRATE 1 gram, 0.03 gram, magnesium sulfate, manganous sulfate 0.01 gram, dipotassium hydrogen phosphate 0.8 gram, 1 gram, sodium carbonate, calcium superphosphate 0.25 gram, 50 grams, agar, water 1000ml, PH nature.
Further, the concrete operations that in described step (1), lactobacillus activates in inclined-plane are: be inoculated on sterilized slant medium III with transfering loop by lactobacillus, afterwards Anaerobic culturel 1-2 days at 37 DEG C; The sterilising temp of described slant medium III is 121 DEG C, sterilization time is 20min; And the component of this slant medium III: peptone 10 grams, extractum carnis 10 grams, yeast powder 5 grams, glucose 5 grams, sodium acetate 5 grams, citric acid diamines 2 grams, tween 80 1ml, dipotassium hydrogen phosphate 2 grams, 0.58 gram, magnesium sulfate, manganous sulfate 0.28 gram, 15 grams, agar, water 1000ml, PH6.5.
Further, the concrete operations that yeast saccharomyces cerevisiae activates in inclined-plane in described step (1) are: be inoculated on sterilized slant medium IV with transfering loop by yeast saccharomyces cerevisiae, aerobic cultivation 3 days at 28-30 DEG C afterwards; The sterilising temp of described slant medium IV is 121 DEG C, sterilization time is 30min; And the component of this slant medium IV: potato 200 grams, sucrose 20 grams, agar 15-20 gram, water 1000ml, PH nature.
The beneficial effect of a kind of compound micro-ecological preparation for improvement of pond water quality of the present invention is: adopt this compound micro-ecological preparation can either improve dissolved oxygen amount in water body, promote the normal microflora in cultural ecological system and beneficial algae activated growth, keep the eubiosis of aquaculture water; The movement in pond and residual bait can be made again to pollute be purified, stablize and improve water quality, thus reducing the COD in water body; Namely compound micro-ecological preparation of the present invention has improvement, optimizes the effect of pond water quality.
[embodiment]
A kind of compound micro-ecological preparation for improvement of pond water quality of the present invention, the concrete steps of its preparation method are as follows:
(1) former strain inclined plane is cultivated: the Rhodopseudomonas palustris in freezing pipe, nitrifier, lactobacillus and yeast saccharomyces cerevisiae are activated in inclined-plane respectively, be placed in 90mm culture dish afterwards respectively and carry out purifying, obtain Rhodopseudomonas palustris slant strains, nitrifier slant strains, lactobacillus slant strains and yeast saccharomyces cerevisiae slant strains, for subsequent use;
(2) former strain liquid is cultivated: Rhodopseudomonas palustris slant strains be inoculated in sterilized liquid nutrient medium I, within illumination cultivation 5-7 days, obtain Rhodopseudomonas palustris original bacteria liquid afterwards in 28 DEG C-30 DEG C anaerobism tengsten lamps; Nitrifier slant strains be inoculated in sterilized liquid nutrient medium II, then at 25 DEG C-28 DEG C, aerobic cultivation obtains nitrifier original bacteria liquid in 3-5 days; Lactobacillus slant strains be inoculated in sterilized liquid nutrient medium III, then at 37 DEG C, Anaerobic culturel 1-2 days obtains lactobacillus original bacteria liquid; Yeast saccharomyces cerevisiae slant strains be inoculated in sterilized liquid nutrient medium IV, at 28 DEG C-30 DEG C, aerobic cultivation obtains yeast saccharomyces cerevisiae original bacteria liquid in 3 days afterwards;
(3) first order seed is cultivated: Rhodopseudomonas palustris original bacteria liquid, nitrifier original bacteria liquid, lactobacillus original bacteria liquid and yeast saccharomyces cerevisiae original bacteria liquid are inoculated in sterilized fermention medium I by the bacterium amount ratio of 2:2:1:1 and cultivate, particularly: first inoculate nitrifier original bacteria liquid, yeast saccharomyces cerevisiae original bacteria liquid aerobic cultivation 3 days at 28 DEG C-30 DEG C, and then inoculate Rhodopseudomonas palustris original bacteria liquid, lactobacillus original bacteria liquid 37 DEG C of Anaerobic culturel 3 days, obtain the first order seed of compound micro-ecological preparation;
(4) secondary seed is cultivated: by the first order seed of gained by 5% inoculum size be inoculated in sterilized fermention medium II, and at 28 DEG C-30 DEG C first aerobic cultivation 3-5 days Anaerobic culturel 3-5 days again, obtain the secondary seed of compound micro-ecological preparation;
(5) finished product is cultivated: by the secondary seed of gained by 5% inoculum size be seeded in sterilized fermention medium III, and at 28 DEG C-30 DEG C first aerobic cultivation 3-5 days Anaerobic culturel 3-5 days again, obtain the finished product of described compound micro-ecological preparation.
Wherein: the concrete operations that in step (1), Rhodopseudomonas palustris activates in inclined-plane are: be inoculated on sterilized slant medium I with transfering loop by Rhodopseudomonas palustris, afterwards in 28 DEG C-30 DEG C anaerobism tengsten lamp illumination cultivation 5-7 days; The sterilising temp of described slant medium I is 121 DEG C, sterilization time is 15min; And the component of this slant medium I: yeast powder 10 grams, dipotassium hydrogen phosphate 1 gram, 0.5 gram, magnesium sulfate, 20 grams, agar, water 1000ml, PH 7.0-7.2.The concrete operations that in step (1), nitrifier activates in inclined-plane are: prepare slant medium II, and sterilizing 20min at slant medium II is placed in 121 DEG C, with transfering loop, nitrifier is inoculated on slant medium II afterwards, then aerobic cultivation 3-5 days at 25 DEG C-28 DEG C; The component of described slant medium II: SODIUMNITRATE 1 gram, 0.03 gram, magnesium sulfate, manganous sulfate 0.01 gram, dipotassium hydrogen phosphate 0.8 gram, 1 gram, sodium carbonate, calcium superphosphate 0.25 gram, 50 grams, agar, water 1000ml, PH nature.The concrete operations that in step (1), lactobacillus activates in inclined-plane are: be inoculated on sterilized slant medium III with transfering loop by lactobacillus, afterwards Anaerobic culturel 1-2 days at 37 DEG C; The sterilising temp of described slant medium III is 121 DEG C, sterilization time is 20min; And the component of this slant medium III: peptone 10 grams, extractum carnis 10 grams, yeast powder 5 grams, glucose 5 grams, sodium acetate 5 grams, citric acid diamines 2 grams, tween 80 1ml, dipotassium hydrogen phosphate 2 grams, 0.58 gram, magnesium sulfate, manganous sulfate 0.28 gram, 15 grams, agar, water 1000ml, PH6.5.The concrete operations that yeast saccharomyces cerevisiae activates in inclined-plane in step (1) are: be inoculated on sterilized slant medium IV with transfering loop by yeast saccharomyces cerevisiae, aerobic cultivation 3 days at 28-30 DEG C afterwards; The sterilising temp of described slant medium IV is 121 DEG C, sterilization time is 30min; And the component of this slant medium IV: potato 200 grams, sucrose 20 grams, agar 15-20 gram, water 1000ml, PH nature.
The component of liquid nutrient medium I in step (2): yeast powder 10 grams, dipotassium hydrogen phosphate 1 gram, 0.5 gram, magnesium sulfate, water 1000ml, PH7.0-7.2; The component of liquid nutrient medium II: SODIUMNITRATE 1 gram, 0.03 gram, magnesium sulfate, manganous sulfate 0.01 gram, dipotassium hydrogen phosphate 0.8 gram, 1 gram, sodium carbonate, calcium superphosphate 0.25 gram, water 1000ml, PH nature; The component of liquid nutrient medium III: peptone 10 grams, extractum carnis 10 grams, yeast powder 5 grams, glucose 5 grams, sodium acetate 5 grams, citric acid diamines 2 grams, tween 80 1ml, dipotassium hydrogen phosphate 2 grams, 0.58 gram, magnesium sulfate, manganous sulfate 0.28 gram, water 1000ml, PH6.5; The component of liquid nutrient medium IV: potato 200 grams, sucrose 20 grams, water 1000ml, PH nature.
The component of fermention medium I in step (3): tangerine water 5%, yeast powder 0.5%, peptone 1%, ammonium chloride 0.2%, sodium-chlor 0.1%, potassium primary phosphate 0.1%, magnesium sulfate 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water.The component of fermention medium II in step (4): tangerine water 5%, yeast powder 0.1%, peptone 0.1%, ammonium chloride 0.2%, sodium-chlor 0.1%, potassium primary phosphate 0.1%, magnesium sulfate 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water.The component of fermention medium III in step (5): tangerine water 5%, ammonium chloride 0.2%, sodium-chlor 0.05%, potassium primary phosphate 0.1%, magnesium sulfate 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water.
It should be noted that, in the present invention, the per-cent of each substratum is mass percent.
Compound micro-ecological preparation of the present invention is for improvement of the water quality optimizing pond, by testing as follows, applicant illustrates that this compound micro-ecological preparation can play optimization pond water quality, particularly:
In certain aquafarm, get two ponds, the configurations such as the area in this two pond, the depth of water, aquaculture model, stocking rate, support equipment and feeding and management condition are all identical, and one, two pond is set to test tank, another is set to contrast pond, in test tank, use compound micro-ecological preparation of the present invention, and amount of application is 400ml/ mu, and to contrast pond be that compound micro-ecological preparation of the present invention is not used in blank pond namely; Test period 20 to July of July 30; Test-results: after testing, test tank is than contrast pond, and the dissolved oxygen amount in its water body improves brings up to 5.1mg/L from 3.2mg/L, namely improves 59%; COD drops to 1700mg/L from 2500mg/L, reduces 32%.Therefore, known by this test-results, adopt compound micro-ecological preparation of the present invention can optimize the water quality of water body in pond, the ecotope of balance aquaculture water, thus promote the healthy growth of aquaculture organism in pond.
To sum up, adopt this compound micro-ecological preparation can either improve dissolved oxygen amount in water body, promote the normal microflora in cultural ecological system and beneficial algae activated growth, keep the eubiosis of aquaculture water; The movement in pond and residual bait can be made again to pollute be purified, stablize and improve water quality, thus reducing the COD in water body; Namely compound micro-ecological preparation of the present invention has improvement, optimizes the effect of pond water quality.
Claims (5)
1. for improvement of a compound micro-ecological preparation for pond water quality, it is characterized in that: the concrete steps of its preparation method are as follows:
(1) former strain inclined plane is cultivated: the Rhodopseudomonas palustris in freezing pipe, nitrifier, lactobacillus and yeast saccharomyces cerevisiae are activated in inclined-plane respectively, be placed in 90mm culture dish afterwards respectively and carry out purifying, obtain Rhodopseudomonas palustris slant strains, nitrifier slant strains, lactobacillus slant strains and yeast saccharomyces cerevisiae slant strains, for subsequent use;
(2) former strain liquid is cultivated: Rhodopseudomonas palustris slant strains be inoculated in sterilized liquid nutrient medium I, within illumination cultivation 5-7 days, obtain Rhodopseudomonas palustris original bacteria liquid afterwards in 28 DEG C-30 DEG C anaerobism tengsten lamps; Nitrifier slant strains be inoculated in sterilized liquid nutrient medium II, then at 25 DEG C-28 DEG C, aerobic cultivation obtains nitrifier original bacteria liquid in 3-5 days; Lactobacillus slant strains be inoculated in sterilized liquid nutrient medium III, then at 37 DEG C, Anaerobic culturel 1-2 days obtains lactobacillus original bacteria liquid; Yeast saccharomyces cerevisiae slant strains be inoculated in sterilized liquid nutrient medium IV, at 28 DEG C-30 DEG C, aerobic cultivation obtains yeast saccharomyces cerevisiae original bacteria liquid in 3 days afterwards;
(3) first order seed is cultivated: Rhodopseudomonas palustris original bacteria liquid, nitrifier original bacteria liquid, lactobacillus original bacteria liquid and yeast saccharomyces cerevisiae original bacteria liquid are inoculated in sterilized fermention medium I by the bacterium amount ratio of 2:2:1:1 and cultivate, particularly: first inoculate nitrifier original bacteria liquid, yeast saccharomyces cerevisiae original bacteria liquid aerobic cultivation 3 days at 28 DEG C-30 DEG C, and then inoculate Rhodopseudomonas palustris original bacteria liquid, lactobacillus original bacteria liquid 37 DEG C of Anaerobic culturel 3 days, obtain the first order seed of compound micro-ecological preparation;
(4) secondary seed is cultivated: by the first order seed of gained by 5% inoculum size be inoculated in sterilized fermention medium II, and at 28 DEG C-30 DEG C first aerobic cultivation 3-5 days Anaerobic culturel 3-5 days again, obtain the secondary seed of compound micro-ecological preparation;
(5) finished product is cultivated: by the secondary seed of gained by 5% inoculum size be seeded in sterilized fermention medium III, and at 28 DEG C-30 DEG C first aerobic cultivation 3-5 days Anaerobic culturel 3-5 days again, obtain the finished product of described compound micro-ecological preparation;
Wherein, the component of liquid nutrient medium I: yeast powder 10 grams, dipotassium hydrogen phosphate 1 gram, 0.5 gram, magnesium sulfate, 20 grams, agar, water 1000ml, PH7.0-7.2; The component of liquid nutrient medium II: SODIUMNITRATE 1 gram, 0.03 gram, magnesium sulfate, manganous sulfate 0.01 gram, dipotassium hydrogen phosphate 0.8 gram, 1 gram, sodium carbonate, calcium superphosphate 0.25 gram, water 1000ml, PH nature; The component of liquid nutrient medium III: peptone 10 grams, extractum carnis 10 grams, yeast powder 5 grams, glucose 5 grams, sodium acetate 5 grams, citric acid diamines 2 grams, tween 80 1ml, dipotassium hydrogen phosphate 2 grams, 0.58 gram, magnesium sulfate, manganous sulfate 0.28 gram, water 1000ml, PH6.5; The component of liquid nutrient medium IV: potato 200 grams, sucrose 20 grams, water 1000ml, PH nature; The component of fermention medium I: tangerine water 5%, yeast powder 0.5%, peptone 1%, ammonium chloride 0.2%, sodium-chlor 0.1%, potassium primary phosphate 0.1%, magnesium sulfate 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water; The component of fermention medium II: tangerine water 5%, yeast powder 0.1%, peptone 0.1%, ammonium chloride 0.2%, sodium-chlor 0.1%, potassium primary phosphate 0.1%, magnesium sulfate 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water; The component of fermention medium III: tangerine water 5%, ammonium chloride 0.2%, sodium-chlor 0.05%, potassium primary phosphate 0.1%, magnesium sulfate 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water.
2. a kind of compound micro-ecological preparation for improvement of pond water quality according to claim 1, it is characterized in that: the concrete operations that in described step (1), Rhodopseudomonas palustris activates in inclined-plane are: be inoculated on sterilized slant medium I with transfering loop by Rhodopseudomonas palustris, afterwards in 28 DEG C-30 DEG C anaerobism tengsten lamp illumination cultivation 5-7 days; The sterilising temp of described slant medium I is 121 DEG C, sterilization time is 15min; And the component of this slant medium I: yeast powder 10 grams, dipotassium hydrogen phosphate 1 gram, 0.5 gram, magnesium sulfate, 20 grams, agar, water 1000ml, PH 7.0-7.2.
3. a kind of compound micro-ecological preparation for improvement of pond water quality according to claim 1, it is characterized in that: the concrete operations that in described step (1), nitrifier activates in inclined-plane are: prepare slant medium II, and sterilizing 20min at slant medium II is placed in 121 DEG C, with transfering loop, nitrifier is inoculated on slant medium II afterwards, then aerobic cultivation 3-5 days at 25 DEG C-28 DEG C; The component of described slant medium II: SODIUMNITRATE 1 gram, 0.03 gram, magnesium sulfate, manganous sulfate 0.01 gram, dipotassium hydrogen phosphate 0.8 gram, 1 gram, sodium carbonate, calcium superphosphate 0.25 gram, 50 grams, agar, water 1000ml, PH nature.
4. a kind of compound micro-ecological preparation for improvement of pond water quality according to claim 1, it is characterized in that: the concrete operations that in described step (1), lactobacillus activates in inclined-plane are: be inoculated on sterilized slant medium III with transfering loop by lactobacillus, afterwards Anaerobic culturel 1-2 days at 37 DEG C; The sterilising temp of described slant medium III is 121 DEG C, sterilization time is 20min; And the component of this slant medium III: peptone 10 grams, extractum carnis 10 grams, yeast powder 5 grams, glucose 5 grams, sodium acetate 5 grams, citric acid diamines 2 grams, tween 80 1ml, dipotassium hydrogen phosphate 2 grams, 0.58 gram, magnesium sulfate, manganous sulfate 0.28 gram, 15 grams, agar, water 1000ml, PH6.5.
5. a kind of compound micro-ecological preparation for improvement of pond water quality according to claim 1, it is characterized in that: the concrete operations that yeast saccharomyces cerevisiae activates in inclined-plane in described step (1) are: be inoculated on sterilized slant medium IV with transfering loop by yeast saccharomyces cerevisiae, aerobic cultivation 3 days at 28-30 DEG C afterwards; The sterilising temp of described slant medium IV is 121 DEG C, sterilization time is 30min; And the component of this slant medium IV: potato 200 grams, sucrose 20 grams, agar 15-20 gram, water 1000ml, PH nature.
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