CN103466910A - Compound micro ecological bacteria agent used for live pig excrement treatment - Google Patents
Compound micro ecological bacteria agent used for live pig excrement treatment Download PDFInfo
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Abstract
The invention provides a compound micro ecological bacteria agent used for the live pig excrement treatment. The preparation method of the compound micro ecological bacteria agent particularly comprises the following steps: nitrogen-fixing bacteria, rhodopseudomonas palustris, bacillus subtilis, lactobacillus acidophilus and saccharomyces cerevisiae all in a freezing storage tube are respectively activated in the slope, and then are purified, so that various slant strains are obtained for standby application; the corresponding original bacterium fluids are prepared by utilizing the slant strains; the original bacterium fluids are inoculated in a sterilized fermentation medium I according to the bacteria amount ratio of 2:2:2:1:1 so as to obtain the first grade seeds of the compound micro ecological bacteria agent; the first grade seeds are inoculated in a sterilized fermentation medium II according to the inoculation amount of 5% so as to obtain the second grade seeds of the compound micro ecological bacteria agent; finally, the second grade seeds are inoculated in a sterilized fermentation medium III according to the inoculation amount of 5% so as to obtain a finished product of the compound micro ecological bacteria agent. Through the adoption of the micro ecological bacteria agent provided by the invention, the poultry excrement is treated, the secondary pollution is avoided, and the organic fertilizer which is higher in fertility and lower in cost can be obtained in a shorter time.
Description
[technical field]
The present invention relates to the compound microecological microbial inoculum that a kind of pig-breeding field is used, relate in particular to a kind of compound microecological microbial inoculum of processing for live pig ight soil.
[background technology]
China is livestock and poultry cultivation big country, and the raising that aquaculture is living standards of the people contributes, and also produces a large amount of feces of livestock and poultry simultaneously.Without processing, just directly enter environment directly endangers ecotope and human health to a large amount of feces of livestock and poultry, affects the sustainable development of livestock and poultry industry, prevents and treats feces of livestock and poultry for this reason and pollutes very urgent.Domestic present processing feces of livestock and poultry be direct returning to farmland or through simple manual handling for field, cause so a large amount of organic losses, not only uneconomical but also pollute surrounding enviroment.
And compost is innoxious, resource utilization feces of livestock and poultry treatment process commonly used at present, the becoming thoroughly decomposed mainly by multiple-microorganism, to decompose and transform organic substance of composting substrate completes, but Natural compost initial stage microbial biomass is few, time that need to be longer could breed, be that efficiency is not ideal enough, fermentation period is longer.
[summary of the invention]
Technical problem to be solved by this invention is to provide a kind of compound microecological microbial inoculum of processing for live pig ight soil, utilizes it can obtain in the short period of time the fertilizer that fertility is higher.
The present invention solves the problems of the technologies described above by the following technical programs: a kind of compound microecological microbial inoculum of processing for live pig ight soil, and the concrete steps of its preparation method are as follows:
(1) former strain inclined plane is cultivated: by the vinelandii in the freezing pipe, Rhodopseudomonas palustris, subtilis, Lactobacterium acidophilum, and yeast saccharomyces cerevisiae in inclined-plane, activate respectively, be placed in respectively afterwards the 90mm culture dish and carry out purifying, obtain vinelandii slant strains, Rhodopseudomonas palustris slant strains, subtilis slant strains, Lactobacterium acidophilum slant strains, reach the yeast saccharomyces cerevisiae slant strains, standby;
(2) former bacterial classification liquid culture: the vinelandii slant strains is inoculated in sterilized liquid nutrient medium I, is placed in afterwards aerobic cultivation under 25 ℃-27 ℃ and obtains the vinelandii original bacteria liquid in 1-2 days; The Rhodopseudomonas palustris slant strains is inoculated in sterilized liquid nutrient medium II, then in 28 ℃ of-30 ℃ of anaerobism tengsten lamp illumination cultivation, within 5-7 days, obtains the Rhodopseudomonas palustris original bacteria liquid; The subtilis slant strains is inoculated in sterilized liquid nutrient medium III, and then under 30 ℃, aerobic cultivation obtains the subtilis original bacteria liquid in 1-2 days; The Lactobacterium acidophilum slant strains is inoculated in sterilized liquid nutrient medium IV, is placed in afterwards 37 ℃ of lower anaerobism cultivations and within 1-2 days, obtains the Lactobacterium acidophilum original bacteria liquid; The yeast saccharomyces cerevisiae slant strains is inoculated in sterilized liquid nutrient medium V, and under 28 ℃-30 ℃, aerobic cultivation obtains the yeast saccharomyces cerevisiae original bacteria liquid in 3 days afterwards;
(3) first order seed is cultivated: by the vinelandii original bacteria liquid, the Rhodopseudomonas palustris original bacteria liquid, the subtilis original bacteria liquid, the Lactobacterium acidophilum slant strains, and the yeast saccharomyces cerevisiae original bacteria liquid is inoculated in sterilized fermention medium I and is cultivated by the bacterium amount ratio of 2:2:2:1:1, particularly: first Azotobacter original bacteria liquid, the subtilis original bacteria liquid, and yeast saccharomyces cerevisiae original bacteria liquid, and aerobic cultivation 3 days under 30 ℃, and then inoculating lactobacillus acidophilus's original bacteria liquid and Rhodopseudomonas palustris original bacteria liquid, in 37 ℃ of lower anaerobism, cultivate 3 days, obtain the first order seed of compound microecological microbial inoculum,
(4) secondary seed is cultivated: the first order seed of gained is inoculated in sterilized fermention medium II by 5% inoculum size, and under 30 ℃ first aerobic cultivations 3-5 days anaerobism cultivation 3-5 days again, obtain the secondary seed of compound microecological microbial inoculum;
(5) finished product is cultivated: the secondary seed of gained is seeded in sterilized fermention medium III by 5% inoculum size, and under 30 ℃ first aerobic 3-5 days again anaerobism cultivate 3-5 days, obtain the finished product of compound microecological microbial inoculum.
Further, the concrete operations that vinelandii activate in inclined-plane in described step (1) are: with transfering loop by inoculating on sterilized slant medium I, aerobic cultivation 1-2 days under 25 ℃-27 ℃ afterwards; The sterilising temp of described slant medium I is that 121 ℃, sterilization time are 20min; And the component of this slant medium I: yeast powder 0.5 gram, N.F,USP MANNITOL 20 grams, potassium primary phosphate 0.2 gram, dipotassium hydrogen phosphate 0.8 gram, sal epsom 0.2 gram, calcium sulfate 0.1 gram, iron(ic) chloride 0.01 gram, Sodium orthomolybdate 0.01 gram, agar 15 grams, water 1000ml, PH7.2.
Further, the concrete operations that in described step (1), Rhodopseudomonas palustris activates in inclined-plane are: with transfering loop, Rhodopseudomonas palustris is inoculated on sterilized slant medium II, afterwards in 28 ℃-30 ℃ anaerobism tengsten lamp illumination cultivation 5-7 days; The sterilising temp of described slant medium II is that 121 ℃, sterilization time are 15min; And the component of this slant medium II: yeast powder 10 grams, dipotassium hydrogen phosphate 1 gram, sal epsom 0.5 gram, agar 20 grams, water 1000ml, PH7.0-7.2.
Further, the concrete operations that in described step (1), subtilis activates in inclined-plane are: with transfering loop, subtilis is inoculated on sterilized slant medium III, afterwards aerobic cultivation 1-2 days under 30 ℃; The sterilising temp of described slant medium III is that 121 ℃, sterilization time are 20min; The component of this slant medium III: peptone 30 grams, glucose 1 gram, extractum carnis 5 grams, sodium-chlor 5 grams, agar 15 grams, water 1000ml, PH7.2.
Further, the concrete operations that in described step (1), Lactobacterium acidophilum activates in inclined-plane are: with transfering loop, Lactobacterium acidophilum is inoculated on sterilized slant medium IV, cultivates 1-2 days in 37 ℃ of lower anaerobism afterwards; The sterilising temp of described slant medium I is that 121 ℃, sterilization time are 20min; And the component of this slant medium I: peptone 10 grams, extractum carnis 10 grams, yeast powder 5 grams, glucose 5 grams, sodium acetate 5 grams, citric acid diamines 2 grams, tween 80 1 gram, dipotassium hydrogen phosphate 2 grams, sal epsom 0.2 gram, manganous sulfate 0.05 gram, calcium carbonate 20 grams, agar 15 grams, water 1000ml, PH6.8.
Further, the concrete operations that yeast saccharomyces cerevisiae activates in inclined-plane in described step (1) are: with transfering loop, yeast saccharomyces cerevisiae is inoculated on sterilized slant medium V to aerobic cultivation 3 days under 28-30 ℃ afterwards; The sterilising temp of described slant medium V is that 121 ℃, sterilization time are 30min; And the component of this slant medium IV: potato 200 grams, sucrose 20 grams, agar 15-20 gram, water 1000ml, PH nature.
Further, the component of liquid nutrient medium I in described step (2): yeast powder 0.5 gram, N.F,USP MANNITOL 20 grams, potassium primary phosphate 0.2 gram, dipotassium hydrogen phosphate 0.8 gram, sal epsom 0.2 gram, calcium sulfate 0.1 gram, iron(ic) chloride 0.01 gram, Sodium orthomolybdate 0.01 gram, water 1000ml, PH7.2; The component of liquid nutrient medium II: yeast powder 10 grams, dipotassium hydrogen phosphate 1 gram, sal epsom 0.5 gram, water 1000ml, PH7.0-7.2; The component of liquid nutrient medium III: peptone 30 grams, glucose 1 gram, extractum carnis 5 grams, sodium-chlor 5 grams, water 1000ml, PH7.2; The component of liquid nutrient medium IV: peptone 10 grams, extractum carnis 10 grams, yeast powder 5 grams, glucose 5 grams, sodium acetate 5 grams, citric acid diamines 2 grams, tween 80 1 gram, dipotassium hydrogen phosphate 2 grams, sal epsom 0.2 gram, manganous sulfate 0.05 gram, calcium carbonate 20 grams, water 1000ml, PH6.8; The component of liquid nutrient medium V: potato 200 grams, sucrose 20 grams, water 1000ml, PH nature.
Further, the component of fermention medium I in described step (3): tangerine water 5%, yeast powder 0.5%, peptone 1%, ammonium chloride 0.1%, sodium-chlor 0.05%, potassium primary phosphate 0.1%, sal epsom 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water.
Further, the component of fermention medium II in described step (4): tangerine water 5%, yeast powder 0.1%, peptone 0.1%, ammonium chloride 0.1%, sodium-chlor 0.1%, potassium primary phosphate 0.05%, sal epsom 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water.
Further, the component of fermention medium III in described step (5): tangerine water 5%, ammonium chloride 0.2%, sodium-chlor 0.05%, potassium primary phosphate 0.1%, sal epsom 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water.
The beneficial effect of a kind of compound microecological microbial inoculum of processing for live pig ight soil of the present invention is: utilize this compound microecological bacteria fermentation to process live pig ight soil, can shorten fermentation period, improve composting efficiency, thereby both processed feces of livestock and poultry, avoided producing secondary pollution, and can obtain in the short period of time again higher the and lower-cost fertilizer of fertility.
[embodiment]
A kind of compound microecological microbial inoculum of processing for live pig ight soil of the present invention, the concrete steps of its preparation method are as follows:
(1) former strain inclined plane is cultivated: by the vinelandii in the freezing pipe, Rhodopseudomonas palustris, subtilis, Lactobacterium acidophilum, and yeast saccharomyces cerevisiae in inclined-plane, activate respectively, be placed in respectively afterwards the 90mm culture dish and carry out purifying, obtain vinelandii slant strains, Rhodopseudomonas palustris slant strains, subtilis slant strains, Lactobacterium acidophilum slant strains, reach the yeast saccharomyces cerevisiae slant strains, standby;
(2) former bacterial classification liquid culture: the vinelandii slant strains is inoculated in sterilized liquid nutrient medium I, is placed in afterwards aerobic cultivation under 25 ℃-27 ℃ and obtains the vinelandii original bacteria liquid in 1-2 days; The Rhodopseudomonas palustris slant strains is inoculated in sterilized liquid nutrient medium II, then in 28 ℃ of-30 ℃ of anaerobism tengsten lamp illumination cultivation, within 5-7 days, obtains the Rhodopseudomonas palustris original bacteria liquid; The subtilis slant strains is inoculated in sterilized liquid nutrient medium III, and then under 30 ℃, aerobic cultivation obtains the subtilis original bacteria liquid in 1-2 days; The Lactobacterium acidophilum slant strains is inoculated in sterilized liquid nutrient medium IV, is placed in afterwards 37 ℃ of lower anaerobism cultivations and within 1-2 days, obtains the Lactobacterium acidophilum original bacteria liquid; The yeast saccharomyces cerevisiae slant strains is inoculated in sterilized liquid nutrient medium V, and under 28 ℃-30 ℃, aerobic cultivation obtains the yeast saccharomyces cerevisiae original bacteria liquid in 3 days afterwards;
(3) first order seed is cultivated: by the vinelandii original bacteria liquid, the Rhodopseudomonas palustris original bacteria liquid, the subtilis original bacteria liquid, the Lactobacterium acidophilum slant strains, and the yeast saccharomyces cerevisiae original bacteria liquid is inoculated in sterilized fermention medium I and is cultivated by the bacterium amount ratio of 2:2:2:1:1, particularly: first Azotobacter original bacteria liquid, the subtilis original bacteria liquid, and yeast saccharomyces cerevisiae original bacteria liquid, and aerobic cultivation 3 days under 30 ℃, and then inoculating lactobacillus acidophilus's original bacteria liquid and Rhodopseudomonas palustris original bacteria liquid, in 37 ℃ of lower anaerobism, cultivate 3 days, obtain the first order seed of compound microecological microbial inoculum,
(4) secondary seed is cultivated: the first order seed of gained is inoculated in sterilized fermention medium II by 5% inoculum size, and under 30 ℃ first aerobic cultivations 3-5 days anaerobism cultivation 3-5 days again, obtain the secondary seed of compound microecological microbial inoculum;
(5) finished product is cultivated: the secondary seed of gained is seeded in sterilized fermention medium III by 5% inoculum size, and under 30 ℃ first aerobic 3-5 days again anaerobism cultivate 3-5 days, obtain the finished product of compound microecological microbial inoculum.
Wherein: the concrete operations that vinelandii activate in inclined-plane in step (1) are: with transfering loop by inoculating on sterilized slant medium I, aerobic cultivation 1-2 days under 25 ℃-27 ℃ afterwards; The sterilising temp of described slant medium I is that 121 ℃, sterilization time are 20min; And the component of this slant medium I: yeast powder 0.5 gram, N.F,USP MANNITOL 20 grams, potassium primary phosphate 0.2 gram, dipotassium hydrogen phosphate 0.8 gram, sal epsom 0.2 gram, calcium sulfate 0.1 gram, iron(ic) chloride 0.01 gram, Sodium orthomolybdate 0.01 gram, agar 15 grams, water 1000ml, PH7.2.The concrete operations that in step (1), Rhodopseudomonas palustris activates in inclined-plane are: with transfering loop, Rhodopseudomonas palustris is inoculated on sterilized slant medium II, afterwards in 28 ℃-30 ℃ anaerobism tengsten lamp illumination cultivation 5-7 days; The sterilising temp of described slant medium II is that 121 ℃, sterilization time are 15min; And the component of this slant medium II: yeast powder 10 grams, dipotassium hydrogen phosphate 1 gram, sal epsom 0.5 gram, agar 20 grams, water 1000ml, PH7.0-7.2.The concrete operations that in step (1), subtilis activates in inclined-plane are: with transfering loop, subtilis is inoculated on sterilized slant medium III, afterwards aerobic cultivation 1-2 days under 30 ℃; The sterilising temp of described slant medium III is that 121 ℃, sterilization time are 20min; The component of this slant medium III: peptone 30 grams, glucose 1 gram, extractum carnis 5 grams, sodium-chlor 5 grams, agar 15 grams, water 1000ml, PH7.2.The concrete operations that Lactobacterium acidophilum activates in inclined-plane are: with transfering loop, Lactobacterium acidophilum is inoculated on sterilized slant medium IV, cultivates 1-2 days in 37 ℃ of lower anaerobism afterwards; The sterilising temp of described slant medium I is that 121 ℃, sterilization time are 20min; And the component of this slant medium I: peptone 10 grams, extractum carnis 10 grams, yeast powder 5 grams, glucose 5 grams, sodium acetate 5 grams, citric acid diamines 2 grams, tween 80 1 gram, dipotassium hydrogen phosphate 2 grams, sal epsom 0.2 gram, manganous sulfate 0.05 gram, calcium carbonate 20 grams, agar 15 grams, water 1000ml, PH6.8.The concrete operations that yeast saccharomyces cerevisiae activates in inclined-plane are: with transfering loop, yeast saccharomyces cerevisiae is inoculated on sterilized slant medium V to aerobic cultivation 3 days under 28-30 ℃ afterwards; The sterilising temp of described slant medium V is that 121 ℃, sterilization time are 30min; And the component of this slant medium IV: potato 200 grams, sucrose 20 grams, agar 15-20 gram, water 1000ml, PH nature.
The component of liquid nutrient medium I in step (2): yeast powder 0.5 gram, N.F,USP MANNITOL 20 grams, potassium primary phosphate 0.2 gram, dipotassium hydrogen phosphate 0.8 gram, sal epsom 0.2 gram, calcium sulfate 0.1 gram, iron(ic) chloride 0.01 gram, Sodium orthomolybdate 0.01 gram, water 1000ml, PH7.2; The component of liquid nutrient medium II: yeast powder 10 grams, dipotassium hydrogen phosphate 1 gram, sal epsom 0.5 gram, water 1000ml, PH7.0-7.2; The component of liquid nutrient medium III: peptone 30 grams, glucose 1 gram, extractum carnis 5 grams, sodium-chlor 5 grams, water 1000ml, PH7.2; The component of liquid nutrient medium IV: peptone 10 grams, extractum carnis 10 grams, yeast powder 5 grams, glucose 5 grams, sodium acetate 5 grams, citric acid diamines 2 grams, tween 80 1 gram, dipotassium hydrogen phosphate 2 grams, sal epsom 0.2 gram, manganous sulfate 0.05 gram, calcium carbonate 20 grams, water 1000ml, PH6.8; The component of liquid nutrient medium V: potato 200 grams, sucrose 20 grams, water 1000ml, PH nature.
The component of fermention medium I in step (3): tangerine water 5%, yeast powder 0.5%, peptone 1%, ammonium chloride 0.1%, sodium-chlor 0.05%, potassium primary phosphate 0.1%, sal epsom 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water.The component of fermention medium II in step (4): tangerine water 5%, yeast powder 0.1%, peptone 0.1%, ammonium chloride 0.1%, sodium-chlor 0.1%, potassium primary phosphate 0.05%, sal epsom 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water.The component of fermention medium III: tangerine water 5%, ammonium chloride 0.2%, sodium-chlor 0.05%, potassium primary phosphate 0.1%, sal epsom 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water.
It should be noted that, in the present invention, the per-cent of each substratum is mass percent.
In order to illustrate that utilizing compound microecological microbial inoculum of the present invention to process live pig ight soil can obtain the fertilizer that fertility is higher in the short period of time, the applicant sets forth explanation with following example, particularly:
On the Fujian Longyan pig farm, clear water dilution by compound microecological microbial inoculum of the present invention with 100 times, be sprayed on afterwards the mixture surface that pig manure and wood chip, rice straw powder, edible fungi residues (part by weight pig manure: other=4:1) form, after fully being uniformly mixed, again mixed pig manure is carried out to pile fermentation, through the fermentation of 5 to 10 days (according to envrionment temperature), finally become soft, black, fragrant, the organic abundant high-quality fertilizer of acid slightly.In order to illustrate that utilizing compound microecological bacteria fermentation of the present invention to process rear this high-quality fertilizer formed has higher fertility, the applicant is detected the nutrient of the forward and backward described mixture that ferments, and detected result is in Table 1.
The table 1 mixture forward and backward nutrient content (%) that ferments
| ? | Organic | Nitrogen | Phosphorus | Potassium |
| Before fermentation | 15.8 | 0.69 | 0.48 | 0.51 |
| After fermentation | 32.6 | 2.76 | 5.2 | 5.3 |
As shown in Table 1, described mixture moiety after this compound micro-ecological preparation is processed fermentation has significant variation: organic content promotes approximately 1 times, and nitrogen nutrient promotes approximately 4 times relatively, and phosphorus nutrients promotes approximately 11 times, and the potassium attainment is divided approximately 10 times of liftings.In addition, because the main raw material of making described high-quality fertilizer is the pig manure that plant self produces, thereby make cost lower.
To sum up, utilize compound microecological bacteria fermentation of the present invention to process live pig ight soil, can shorten fermentation period, improve composting efficiency, thereby both processed feces of livestock and poultry, avoided producing secondary pollution, can obtain in the short period of time again higher the and lower-cost fertilizer of fertility, for the production of grain, vegetables, lawn, flowers etc. provides the good source of manure.
Claims (10)
1. a compound microecological microbial inoculum of processing for live pig ight soil, it is characterized in that: the concrete steps of its preparation method are as follows:
(1) former strain inclined plane is cultivated: by the vinelandii in the freezing pipe, Rhodopseudomonas palustris, subtilis, Lactobacterium acidophilum, and yeast saccharomyces cerevisiae in inclined-plane, activate respectively, be placed in respectively afterwards the 90mm culture dish and carry out purifying, obtain vinelandii slant strains, Rhodopseudomonas palustris slant strains, subtilis slant strains, Lactobacterium acidophilum slant strains, reach the yeast saccharomyces cerevisiae slant strains, standby;
(2) former bacterial classification liquid culture: the vinelandii slant strains is inoculated in sterilized liquid nutrient medium I, is placed in afterwards aerobic cultivation under 25 ℃-27 ℃ and obtains the vinelandii original bacteria liquid in 1-2 days; The Rhodopseudomonas palustris slant strains is inoculated in sterilized liquid nutrient medium II, then in 28 ℃ of-30 ℃ of anaerobism tengsten lamp illumination cultivation, within 5-7 days, obtains the Rhodopseudomonas palustris original bacteria liquid; The subtilis slant strains is inoculated in sterilized liquid nutrient medium III, and then under 30 ℃, aerobic cultivation obtains the subtilis original bacteria liquid in 1-2 days; The Lactobacterium acidophilum slant strains is inoculated in sterilized liquid nutrient medium IV, is placed in afterwards 37 ℃ of lower anaerobism cultivations and within 1-2 days, obtains the Lactobacterium acidophilum original bacteria liquid; The yeast saccharomyces cerevisiae slant strains is inoculated in sterilized liquid nutrient medium V, and under 28 ℃-30 ℃, aerobic cultivation obtains the yeast saccharomyces cerevisiae original bacteria liquid in 3 days afterwards;
(3) first order seed is cultivated: by the vinelandii original bacteria liquid, the Rhodopseudomonas palustris original bacteria liquid, the subtilis original bacteria liquid, the Lactobacterium acidophilum slant strains, and the yeast saccharomyces cerevisiae original bacteria liquid is inoculated in sterilized fermention medium I and is cultivated by the bacterium amount ratio of 2:2:2:1:1, particularly: first Azotobacter original bacteria liquid, the subtilis original bacteria liquid, and yeast saccharomyces cerevisiae original bacteria liquid, and aerobic cultivation 3 days under 30 ℃, and then inoculating lactobacillus acidophilus's original bacteria liquid and Rhodopseudomonas palustris original bacteria liquid, in 37 ℃ of lower anaerobism, cultivate 3 days, obtain the first order seed of compound microecological microbial inoculum,
(4) secondary seed is cultivated: the first order seed of gained is inoculated in sterilized fermention medium II by 5% inoculum size, and under 30 ℃ first aerobic cultivations 3-5 days anaerobism cultivation 3-5 days again, obtain the secondary seed of compound microecological microbial inoculum;
(5) finished product is cultivated: the secondary seed of gained is seeded in sterilized fermention medium III by 5% inoculum size, and under 30 ℃ first aerobic 3-5 days again anaerobism cultivate 3-5 days, obtain the finished product of compound microecological microbial inoculum.
2. a kind of compound microecological microbial inoculum of processing for live pig ight soil according to claim 1, it is characterized in that: the concrete operations that vinelandii activate in inclined-plane in described step (1) are: with transfering loop by inoculating on sterilized slant medium I, aerobic cultivation 1-2 days under 25 ℃-27 ℃ afterwards; The sterilising temp of described slant medium I is that 121 ℃, sterilization time are 20min; And the component of this slant medium I: yeast powder 0.5 gram, N.F,USP MANNITOL 20 grams, potassium primary phosphate 0.2 gram, dipotassium hydrogen phosphate 0.8 gram, sal epsom 0.2 gram, calcium sulfate 0.1 gram, iron(ic) chloride 0.01 gram, Sodium orthomolybdate 0.01 gram, agar 15 grams, water 1000ml, PH7.2.
3. a kind of compound microecological microbial inoculum of processing for live pig ight soil according to claim 1, it is characterized in that: the concrete operations that in described step (1), Rhodopseudomonas palustris activates in inclined-plane are: with transfering loop, Rhodopseudomonas palustris is inoculated on sterilized slant medium II, afterwards in 28 ℃-30 ℃ anaerobism tengsten lamp illumination cultivation 5-7 days; The sterilising temp of described slant medium II is that 121 ℃, sterilization time are 15min; And the component of this slant medium II: yeast powder 10 grams, dipotassium hydrogen phosphate 1 gram, sal epsom 0.5 gram, agar 20 grams, water 1000ml, PH7.0-7.2.
4. a kind of compound microecological microbial inoculum of processing for live pig ight soil according to claim 1, it is characterized in that: the concrete operations that in described step (1), subtilis activates in inclined-plane are: with transfering loop, subtilis is inoculated on sterilized slant medium III, afterwards aerobic cultivation 1-2 days under 30 ℃; The sterilising temp of described slant medium III is that 121 ℃, sterilization time are 20min; The component of this slant medium III: peptone 30 grams, glucose 1 gram, extractum carnis 5 grams, sodium-chlor 5 grams, agar 15 grams, water 1000ml, PH7.2.
5. a kind of compound microecological microbial inoculum of processing for live pig ight soil according to claim 1, it is characterized in that: the concrete operations that in described step (1), Lactobacterium acidophilum activates in inclined-plane are: with transfering loop, Lactobacterium acidophilum is inoculated on sterilized slant medium IV, cultivates 1-2 days in 37 ℃ of lower anaerobism afterwards; The sterilising temp of described slant medium I is that 121 ℃, sterilization time are 20min; And the component of this slant medium I: peptone 10 grams, extractum carnis 10 grams, yeast powder 5 grams, glucose 5 grams, sodium acetate 5 grams, citric acid diamines 2 grams, tween 80 1 gram, dipotassium hydrogen phosphate 2 grams, sal epsom 0.2 gram, manganous sulfate 0.05 gram, calcium carbonate 20 grams, agar 15 grams, water 1000ml, PH6.8.
6. a kind of compound microecological microbial inoculum of processing for live pig ight soil according to claim 1, it is characterized in that: the concrete operations that yeast saccharomyces cerevisiae activates in inclined-plane in described step (1) are: with transfering loop, yeast saccharomyces cerevisiae is inoculated on sterilized slant medium V to aerobic cultivation 3 days under 28-30 ℃ afterwards; The sterilising temp of described slant medium V is that 121 ℃, sterilization time are 30min; And the component of this slant medium IV: potato 200 grams, sucrose 20 grams, agar 15-20 gram, water 1000ml, PH nature.
7. a kind of compound microecological microbial inoculum of processing for live pig ight soil according to claim 1, is characterized in that: the component of liquid nutrient medium I in described step (2): yeast powder 0.5 gram, N.F,USP MANNITOL 20 grams, potassium primary phosphate 0.2 gram, dipotassium hydrogen phosphate 0.8 gram, sal epsom 0.2 gram, calcium sulfate 0.1 gram, iron(ic) chloride 0.01 gram, Sodium orthomolybdate 0.01 gram, water 1000ml, PH7.2; The component of liquid nutrient medium II: yeast powder 10 grams, dipotassium hydrogen phosphate 1 gram, sal epsom 0.5 gram, water 1000ml, PH7.0-7.2; The component of liquid nutrient medium III: peptone 30 grams, glucose 1 gram, extractum carnis 5 grams, sodium-chlor 5 grams, water 1000ml, PH7.2; The component of liquid nutrient medium IV: peptone 10 grams, extractum carnis 10 grams, yeast powder 5 grams, glucose 5 grams, sodium acetate 5 grams, citric acid diamines 2 grams, tween 80 1 gram, dipotassium hydrogen phosphate 2 grams, sal epsom 0.2 gram, manganous sulfate 0.05 gram, calcium carbonate 20 grams, water 1000ml, PH6.8; The component of liquid nutrient medium V: potato 200 grams, sucrose 20 grams, water 1000ml, PH nature.
8. a kind of compound microecological microbial inoculum of processing for live pig ight soil according to claim 1, it is characterized in that: the component of fermention medium I in described step (3): tangerine water 5%, yeast powder 0.5%, peptone 1%, ammonium chloride 0.1%, sodium-chlor 0.05%, potassium primary phosphate 0.1%, sal epsom 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water.
9. a kind of compound microecological microbial inoculum of processing for live pig ight soil according to claim 1, it is characterized in that: the component of fermention medium II in described step (4): tangerine water 5%, yeast powder 0.1%, peptone 0.1%, ammonium chloride 0.1%, sodium-chlor 0.1%, potassium primary phosphate 0.05%, sal epsom 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water.
10. a kind of compound microecological microbial inoculum of processing for live pig ight soil according to claim 1, it is characterized in that: the component of fermention medium III in described step (5): tangerine water 5%, ammonium chloride 0.2%, sodium-chlor 0.05%, potassium primary phosphate 0.1%, sal epsom 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103843974A (en) * | 2014-03-25 | 2014-06-11 | 农业部环境保护科研监测所 | Compound microecologics and application thereof |
| CN105347858A (en) * | 2015-11-17 | 2016-02-24 | 张山虎 | Microbe composite fermentation material used for substance decomposed fermentation and preparation method thereof |
| CN107723254A (en) * | 2016-08-10 | 2018-02-23 | 湖南山河美生物环保科技股份有限公司 | A kind of biological organic fertilizer zymophyte for discarded object fermentation process |
| CN111808764A (en) * | 2019-04-11 | 2020-10-23 | 河北维尔利动物药业集团有限公司 | Preparation method of bacillus subtilis composite liquid microbial inoculum for preventing and treating epidemic diseases of livestock and poultry |
| CN112795514A (en) * | 2021-02-05 | 2021-05-14 | 北京普利赛环保科技发展有限责任公司 | Low-temperature microbial treatment composite powder suitable for dry latrine in cold area and preparation method thereof |
| CN113337420A (en) * | 2021-05-06 | 2021-09-03 | 广东丽豪生物农业有限公司 | Composite nitrogen-fixing microbial agent and preparation method and application thereof |
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| CN103843974A (en) * | 2014-03-25 | 2014-06-11 | 农业部环境保护科研监测所 | Compound microecologics and application thereof |
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| CN107723254A (en) * | 2016-08-10 | 2018-02-23 | 湖南山河美生物环保科技股份有限公司 | A kind of biological organic fertilizer zymophyte for discarded object fermentation process |
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| CN112795514A (en) * | 2021-02-05 | 2021-05-14 | 北京普利赛环保科技发展有限责任公司 | Low-temperature microbial treatment composite powder suitable for dry latrine in cold area and preparation method thereof |
| CN113337420A (en) * | 2021-05-06 | 2021-09-03 | 广东丽豪生物农业有限公司 | Composite nitrogen-fixing microbial agent and preparation method and application thereof |
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