CN103349758B - The Chinese medicine preparation of a kind of aquatic animal hepatoprotective and preparation method - Google Patents
The Chinese medicine preparation of a kind of aquatic animal hepatoprotective and preparation method Download PDFInfo
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Abstract
The Chinese medicine preparation of a kind of aquatic animal hepatoprotective and preparation method, a kind of Chinese medicine preparation of aquatic animal hepatoprotective, it is made up of turmeric paste, a certain proportion of raw material of corn starch, its step: A, takes Rhizoma Curcumae Longae medicinal powder, with 500 mL 80%v/v alcohol reflux；B, filtration extracting solution, merge, concentrate with Rotary Evaporators；C, it is transferred in beaker, uniform temperature, adds corn starch mix homogeneously；D, it is placed in baking oven again, is dried under uniform temperature；E, taking-up dry drug sieve, and have obtained Chinese medicine preparation.The present invention protect aquatic animal liver not by or alleviate damage, it is to avoid cause concurrent Other diseases because of hepar damnification, decrease the generation of aquatic animal disease.Plant-derived medicine, safely and effectively, curcumin, total curcumin content that the method is extracted are high, and formula is reasonable, effective, easy to implement the method, easy to operate, is particularly suited for intensive, the needs of large-scale cultivation of mixed feed in aquaculture.
Description
Technical field
The invention belongs to aquatic animal disease preventing and treating (fisheries drug) technical field, be more particularly to a kind of aquatic animal and protect
The Chinese medicine preparation of protecting liver, also relates to the preparation method of a kind of aquatic animal hepatoprotective Chinese medicine preparation.
Background technology
" Fish hepatopathy " (The liver disease of Fishes) is a kind of common in aquaculture in recent years and danger
Hepar damnification that the bigger parasitics factor non-infectious, non-of evil causes, hepatic insufficiency disease, it has become as sea, light
One of serious disease of the main cultured fishes of water.The disease such as hepatic injury and liver and gallbladder syndrome is once there is in intensive culture
Disease, often presents a kind of extensive and ill phenomena of mortality of globality, and mortality rate may be up to 80%-90%, although this kind of disease
Sick without infectiousness, but its harm and consequence are often considerably beyond infectious disease, have especially increased the weight of the harm of infectious disease
Degree.In particular with the high speed development of cultured fishes intensive culture, the improving rapidly of cultivation density, the hand fit that throws something and feeds raises
Material, the deterioration increasingly of use medicine lack of standardization, water body environment, the unbalance and disappearance of feedstuff Middle nutrition composition and feedstuff exist
The adverse effect factors such as noxious substance get more and more, and all may cause damage fish liver, cause the water such as current cultivation fish and shrimp
Produce animal hepatopathy and occur extremely universal, cause cultured fishes hepatic injury phenomenon the most increasingly severe.Therefore, for cultured fishes
Hepatic injury phenomenon, develop a kind of efficiently, low toxicity, noresidue can improve fish body immunity, the new drug preparation of hepatoprotective seems
Particularly significant.It has been investigated that, curcumin can not only the liver protecting injury-free, and also have repair hepatic injury work
With.
Rhizoma Curcumae Longae (Curcuma Longa L.) derives from the dry rhizome of zingiberaceous plant Rhizoma Curcumae Longae, mainly originate in China Sichuan,
The ground such as Yunnan, Guangxi, Guangdong, Fujian, Taiwan.Rhizoma Curcumae Longae is warm in nature, acrid in the mouth, hardship, has the effect of removing blood stasis circulation of qi promoting, relieving pain of dysmenorrhea, often
For breast side of body twinge, amenorrhea, abdominal mass, rheumatism shoulder arm pain, tumbling and swelling etc.[1]([1] Chinese Pharmacopoeia Commission. the China people are altogether
With state's pharmacopeia () [S]. Beijing: Chemical Industry Press, 2005,186.) curcumin (curcumin) is to carry from Rhizoma Curcumae Longae
The fat-soluble phenol sulfonate of one taken out, is the main active of Rhizoma Curcumae Longae.Research shows, curcumin has antitumor, resists
Many pharmacological actions such as microorganism, removing free radical, it is most important that Rhizoma Curcumae Longae has antiinflammatory, anti peroxidation of lipid, protection
Hepatocyte is from the effect of damage[2,3]([2] Xu Donghui, Wang Sheng, Jin Jing etc. the Advance on Pharmacological Activities [J] of curcumin. in
Medical herbs, 2005,36 (11): 1737-1740. [3] Shu Jianchang, Pan Jie. curcumin effect of anti hepatic fibrosis and the research of mechanism thereof
Progress [J]. international disease for digest magazine, 2007,27 (4): 239-241.) vast stretch of wooded country swallow etc.[4]([4] vast stretch of wooded country swallow, Jian Hua perhaps, Wen Cai
Rosy clouds, Wu Lixian. the curcumin solid dispersion protective effect [J] to mouse experiment chronic hepatic injury. Pharmacology and Clinics of Chinese Materia Medica,
2007,23 (2): 25-26.) research shows that curcumin solid dispersion is to CCl4The caused protected work of murine chronic hepatic injury
With.ALT, the AST raised in murine chronic hepatic injury can be reduced, improve liver spleen weight ratio, alleviate liver histopathology and change.
Liu Yong is firm[5]([5] Liu Yonggang, Liu Yongzhong, Wang Xiaodong. curcumin Hepar Mus sternzellen oxidative stress lipid peroxidation
Research [J]. Chinese patent medicine, 2004,26 (10): 829-832.) research confirm curcumin in vivo, external to CCl4Caused
Hepatic injury has significant protective effect, can significantly reduce CCl4Cause original cuiture rat ALT, LDH, MDA level of damage,
And improve the level of SOD and GSH-PX, improve the body Scavenging activity to free radical, thus alleviate it and body cell is attacked
Hit.
Summary of the invention
It is an object of the invention to there are provided the Chinese medicine preparation of a kind of aquatic animal hepatoprotective, protection Aquatic product moves
Thing liver not by or alleviate damage, it is to avoid cause concurrent Other diseases because of hepar damnification, decrease aquatic animal disease
Occur.Plant-derived medicine, the problem that there is not environmental pollution and drug resistance, safely and effectively.
Another object of the present invention is to there are provided a kind of aquatic animal hepatoprotective Turmeric extractum to dissipate
Preparation method.Curcumin, total curcumin content that the method is extracted are high, and formula is reasonable, effective, and the method is easy, behaviour
Facilitate, be particularly suited for intensive, the needs of large-scale production of current aquaculture.
In order to achieve the above object, the present invention uses techniques below measure:
A kind of Chinese medicine preparation of aquatic animal hepatoprotective, it is made up of the raw material of following weight portion:
Parts by weight of raw materials
Crude drug amount (turmeric paste) 1-10
Adjuvant amount (corn starch) 1-12.
A kind of Chinese medicine preparation of aquatic animal hepatoprotective, it is made up by weight of following raw materials according:
Parts by weight of raw materials
Crude drug amount (turmeric paste) 2-9
Adjuvant amount (corn starch) 2-11.
A kind of Chinese medicine preparation of aquatic animal hepatoprotective, it is made up by weight of following raw materials according:
Parts by weight of raw materials
Crude drug amount (turmeric paste) 3-8
Adjuvant amount (corn starch) 3-10.
A kind of Chinese medicine preparation of aquatic animal hepatoprotective, it is made up by weight of following raw materials according:
Parts by weight of raw materials
Crude drug amount (turmeric paste) 4-7
Adjuvant amount (corn starch) 4-9.
A kind of Chinese medicine preparation of aquatic animal hepatoprotective, it is made up by weight of following raw materials according:
Parts by weight of raw materials
Crude drug amount (turmeric paste) 5-6.5
Adjuvant amount (corn starch) 5-8.
A kind of Chinese medicine preparation of aquatic animal hepatoprotective, it is made up by weight of following raw materials according:
Parts by weight of raw materials
Crude drug amount (turmeric paste) 5.8-6.2
Adjuvant amount (corn starch) 6-7.
A kind of Chinese medicine preparation of aquatic animal hepatoprotective, it is made up by weight of following raw materials according:
Parts by weight of raw materials
Crude drug amount (turmeric paste) 5
Adjuvant amount (corn starch) 7.5.
The preparation method of a kind of aquatic animal hepatoprotective Chinese medicine preparation, the steps include:
1,50g Rhizoma Curcumae Longae medicinal powder (30-50 mesh) is taken, with 500mL80%(v/v) alcohol reflux 2-4 time, every time
80-100 minute；
2, filter extracting solution, merge, be concentrated into 500mL with Rotary Evaporators, it is thus achieved that Rhizoma Curcumae Longae extract；
3, being transferred in beaker by Rhizoma Curcumae Longae extract, 78-82 DEG C of water-bath volatilizes to 250mL, it is thus achieved that turmeric paste, adds
Enter 400-500g corn starch mix homogeneously；
4, it is placed in again in baking oven, at 78-82 DEG C, is dried 9-11h；
5, take out dry drug and cross No. 6 sieves, finally give the Chinese medicine preparation (turmeric paste dissipates) of hepatoprotective.
Described Rhizoma Curcumae Longae is a kind of perennial dulcet herbaceous plant, and existing medical value can make again food seasoning.
Xin Xiang is pastel, slightly Fructus Piperis, Moschus taste and Fructus Citri sinensis and Rhizoma Zingiberis Recens mix taste, slightly pungent, bitterness.
The curcuma powder that the dry rhizome of the zingiberaceous plant Rhizoma Curcumae Longae Curcuma longa L. originating in India is milled into, is used as
Flavoring agent and yellow colorants, be the common flavouring agent of family's use, for curry powder, flavouring agent etc..Rhizoma Curcumae Longae be one for many years
Raw dulcet herbaceous plant, existing medical value, food seasoning can be made again.Xin Xiang is pastel, slightly Fructus Piperis, Moschus taste and sweet
Orange mixes taste with Rhizoma Zingiberis Recens, slightly pungent, bitterness.
Rhizoma Curcumae Longae is irregular oval, cylinder or stricture of vagina capitate, often bends, the short dichotomy of some tools, and long 2～5cm, directly
Footpath 1～3cm, surface buff, coarse, there are shrinkage texture and obvious link, and have circular branch trace and mark of fibrous root.Matter is solid,
Being not easily broken, section brown color, to golden yellow, cutin sample, has waxy gloss.Endodermis ring grain is obvious, and vascular bundle is that point-like dissipates
?.Gas perfume (or spice) is special, bitter in the mouth, pungent.
Curcumin can suppress the growth of some position cancerous cell of human body, uses " curcumin " and other chemotherapies
Method can effectively suppress the diffusion of the cancerous cell such as breast carcinoma.In experiment, the scientist of University of Texas is suffered from breast carcinoma
60 white mice done tumor resection, the most respectively give these white mice take " curcumin ".The most after a while
Afterwards, the probability that the white mice containing " curcumin " during scientist finds diet every day suffers from breast cancer is substantially little less than other
White mouse.
Curcumin can be extracted with curcuma powder and that is to say curcumin, be a kind of natural, nontoxic functional pigmented, quilt
Many authorized by states can add the fields such as food, medicine, cosmetics to and be that not limit consumption is to measure as required
Add.
Curcuma powder is that crude drug is pulverized gained, the turmeric paste of the present invention be crude drug Rhizoma Curcumae Longae is pulverized 50 mesh after, logical
Crossing the ethanol extraction by 10 times amount 80%, extract three times, each 1.5h gained, it is by turmeric paste that the turmeric paste of the present invention dissipates
Prepare with starch mixture by a certain percentage.
The present invention compared with prior art, has the following advantages and effect:
A, first Rhizoma Curcumae Longae extract is applied to the hepatoprotective of aquatic animal；
B, preferred alcohol extracting method, effective ingredient curcumin and total curcumin yield are high, effective；
C, can effectively solve the problem that the hepatic injury problem caused because of problems such as mixed feeds, to the hepatic injury such as Ctenopharyngodon idellus, Carassius auratus
Prevention effective percentage reaches 97.5%, and cure rate reaches 90%；
D, method are simple to operate, easy, Chinese medicine powder, mix to raise and throw something and feed, easily operated in producing reality.
E, plant-based medicine, safe without toxic side effect, pollution-free to water environment, there is not drug resistance problems.
Accompanying drawing explanation
Fig. 1 a is a kind of hepatic injury Ctenopharyngodon idellus and Liver of Ctenopharyngodon Idellus microstructure (HE dyeing × 400) after taking curcumin
Fig. 1 b is a kind of hepatic injury Ctenopharyngodon idellus and Liver of Ctenopharyngodon Idellus microstructure (HE dyeing × 400) after taking curcumin
A: suffer from liver and gallbladder syndrome Liver of Ctenopharyngodon Idellus micro-structure diagram；B: take curcumin Liver of Ctenopharyngodon Idellus micro-structure diagram." ":
Nucleus deviation cell is central；“”Cellular morphology disappears；" ← ": vacuolar degeneration.
Detailed description of the invention:
Below in conjunction with specific embodiment, the present invention is described further.
Embodiment 1:
A kind of Chinese medicine preparation of aquatic animal hepatoprotective, it is made up by weight of following raw materials according:
Parts by weight of raw materials
Turmeric paste 3.5
Corn starch 8.5
The preparation method of a kind of aquatic animal hepatoprotective Chinese medicine preparation, the steps include:
A, by Rhizoma Curcumae Longae medicinal powder 500mL80%(v/v) alcohol reflux 2 or 3 or 4 times, each 80 or 85 or 90 or
95 or 100 minutes；
B, filtration extracting solution, merge, be concentrated into 500mL with Rotary Evaporators, it is thus achieved that Rhizoma Curcumae Longae extract；
C, being transferred in beaker by Rhizoma Curcumae Longae extract, 80 DEG C of water-baths volatilize to 250mL, it is thus achieved that turmeric paste, add
400g corn starch mix homogeneously；
D, it is placed in baking oven again, at 78 or 79 or 80 or 81 or 82 DEG C, is dried 9 or 10 or 11h；
E, taking-up dry drug cross No. 6 sieves, finally give the Chinese medicine preparation (turmeric paste dissipates) of hepatoprotective.
Any one weight portion in described curcuma powder mixes mutually with any one weight portion in another kind of corn starch
Close, all can obtain the compositions of this medicine a kind of.Mixed ratio is:
| Embodiment | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 |
| Turmeric paste | 1 | 2 | 3 | 4 | 5 | 5 | 7 | 7.5 | 8 | 8.5 | 9 | 9.5 | 10 |
| Corn starch | 12 | 11 | 10 | 9 | 8 | 7 | 6 | 5.5 | 4 | 2.5 | 2 | 1.5 | 1 |
| Amount to | 13 | 13 | 13 | 13 | 13 | 13 | 13 | 13 | 12 | 11 | 11 | 11 | 11 |
Effect assessment:
1, turmeric paste dissipates grass carp liver injury repairing Effect study:
1.1 test fish and medicines:
Test Ctenopharyngodon idellus is purchased from Qing Ling township of Wuhan City fish seeding ground, average weight (200 ± 5) g.Select outward appearance healthy and strong, travelling alive
Sprinkle, fish is tested in atraumatic conduct, dissects internal organs and all suffers from liver and gallbladder syndrome in various degree.
Trial drug turmeric paste dissipates, curcumin content 1.349mg/g, and Hubei TCM Universities provides；Control drug liver and gall
Li Kang dissipates, and Wuhan Chopper Fishery Bio-Tech Co., Ltd. produces, product batch number 130304.
1.2 test packet and raisings:
Test is carried out in the aquarium that volume is 98cm × 60cm × 100cm, often group set 3 parallel, each parallel put
Fish 20 tail.Water source is the tap water after aeration dechlorination, water temperature average out to (25.8 ± 2.8) DEG C, and dissolved oxygen in water is more than 7mg/L,
PH value is 6.80 ± 0.01.Medicaments uniformity is mixed to raise and is thrown something and fed, and the feedstuff addition that administration group turmeric paste dissipates is 1 ‰, control drug
The feedstuff addition that group liver and gall Li Kang dissipate is 2 ‰, and blank group is thrown something and fed normal feedstuff, respectively throws during during every morning 9 and afternoon 4
Feed once, the feeding volume of 5%, feed 7d continuously.
The mensuration of 1.3 Biochemical Indices In Serums:
During off-test, every case takes fish 5 tail at random, tail venous blood sampling, under room temperature after solidifying, and 3000r/ under the conditions of 4 DEG C
Min is centrifuged 10min, merges upper serum standby.Glutamate pyruvate transaminase (AST) and glutamic oxaloacetic transaminase, GOT (ALT) day Benson Meikang
(Sysmex) automatic clinical chemistry analyzer (Chemix-800) measures, in Changjiang Aquatic Products Inst., Chinese Academy of Aquatic Products Sciences
Carry out；Lactic acid dehydrogenase (LDH), total number born (SOD), catalase (CAT), glutathion peroxidase
(GSH-PX), malonaldehyde (MDA), use Nanjing to build up the detection kit of bio tech ltd and be measured.
1.4 Histopathological Studies:
At the end of experiment, take Ctenopharyngodon idellus fritter liver organization, be placed in BouinShi fixative and fix 3h.Scrubbed, dehydration,
Transparent, permeate with embedding after, be cut into 5 μ m-thick thin slices, after paster, dye with HE staining, then through dehydration and transparent after use
Neutral gum mounting, basis of microscopic observation.
1.5 data analysiss:
Use spss17.0 statistical analysis software to carry out one factor analysis of variance and LSD multiple comparisons carries out statistical analysis.
2, result:
2.1 perusals:
Suffering from liver and gallbladder syndrome Ctenopharyngodon idellus, outward appearance is normal, finds that hepatopancrease color is thin out after dissection, or in yellow, part is in green
Color, frangible.After throwing something and feeding containing curcumin feedstuff 7d, dissect find Liver of Ctenopharyngodon Idellus uniform color, in blood red, minority presents Huang
Color.Liver and gall Li Kang dissipate group similar phenomena, and blank group Liver of Ctenopharyngodon Idellus color is turned white, jaundice situation is serious.
2.2 Biochemical Indices In Serums:
By table 1, table 2 it can be seen that compared with blank group, under curcumin effect, MDA content, AST, ALT and LDH
Activity all significantly reduces (p ＜ 0.01)；SOD, CAT and GSH-PX activity all dramatically increases (p ＜ 0.01)；Turmeric paste dissipates group
MDA content, AST activity is significantly less than positive controls (p ＜ 0.05), and SOD, CAT and GSH-PX activity is noticeably greater than positive right
According to group (p ＜ 0.05).
AST, ALT and LDH activity change (n=15) in table 1 Grass Carp Serum
Note: represent that significance, lower case difference represent significant difference (p ＜ 0.05) with Superscript letters difference in string,
Capitalization difference represents that difference is extremely notable (p ＜ 0.01).
T-SOD, CAT, GSH-PX and MDA activity change (n=15) in table 2 Grass Carp Serum
Note: represent that significance, lower case difference represent significant difference (p ＜ 0.05) with Superscript letters difference in string,
Capitalization difference represents that difference is extremely notable (p ＜ 0.01).
2.3 Histopathological Studies:
Hepatic tissue section shows: suffer from the grass carp hepatocyte core off-center that liver syndrome is sick, boundary between hepatocyte cell
Unclear, part occurs that hepatocyte form disappears, and cell membrane, nucleus can not be differentiated, graininess degeneration, vacuolar degeneration (a).Use
Ctenopharyngodon idellus after turmeric paste is scattered, hepatic cords, centered by central vein, is arranged radially to surrounding；Between hepatocyte, boundary is clear
Chu, cellular morphology is normal, and the nucleus of most cells is positioned at cell central authorities, the only nucleus offset from center of a few cell
(b).As seen from Figure 1, the scattered pathological change to hepatic injury grass carp liver tissue of turmeric paste improves significantly.
3, discuss:
The principle active component that turmeric paste dissipates is curcumin, and curcumin (curcumin) is from Rhizoma Curcumae Longae (Curcuma
Longa L.) in the fat-soluble phenol sulfonate of one that extracts, be the main active of Rhizoma Curcumae Longae.Research shows, curcumin
There is many pharmacological actions such as antitumor, antimicrobial, removing free radical[2,3].Its to the protective effect of liver little
The research of Mus is confirmed[2,6]([2] Xu Donghui, Wang Sheng, Jin Jing etc. the Advance on Pharmacological Activities [J] of curcumin. medium-height grass
Medicine, 2005,36 (11): 1737-1740. [6] vast stretch of wooded country swallow, perhaps Jian Huas, temperature rosy clouds, etc. curcumin solid dispersion is real to mice
The protective effect [J] of the property tested chronic hepatic injury. Pharmacology and Clinics of Chinese Materia Medica, 2007,23 (2): 25-26.), have not yet to see
Applied research above Fish, has investigated the repair that grass carp liver is damaged by curcumin first.
Taking after turmeric paste dissipates one week, in Grass Carp Serum, AST, ALT and LDH content is remarkably decreased (p ＜ 0.01).Generally
When hepatic injury occurs, in blood, three kinds of enzyme content can rise, and may determine that the health status of liver by them[7]([7] are yellow
Triumphant, Yang Hongkun, Gan Hui, etc. feedstuff adds the effect [J] that choline preventing tilapia fatty liver disease becomes. Chinese aquatic science,
2007,14 (2): 257-262.), the decline of three's content, reflect Liver of Ctenopharyngodon Idellus damage and be restored.Antioxygen in Grass Carp Serum
Changing enzyme SOD, CAT and GSH-PX activity to be obviously enhanced (p ＜ 0.01), lipid peroxidation product MDA content significantly reduces (p ＜
0.01), illustrating under curcumin effect, Ctenopharyngodon idellus body is removed Free Radical Activity and is strengthened, and hepatic antioxidative function is restored.Push away
Disconnected turmeric paste dissipates by strengthening Ctenopharyngodon idellus body anti peroxidation of lipid, the ability of free radical resisting, it is to avoid liver is the most impaired, from
And promote body self-regeneration[5,8]([5] Liu Yonggang, Liu Yongzhong, Wang Xiaodong. curcumin Hepar Mus sternzellen oxidative stress fat
The research [J] of matter peroxidation. Chinese patent medicine, 2004,26 (10): 829-832. [8] Pulla Reddy A Ch, Lokesh B
R.Effect of curcumin and eugenol on iron-induced hepatic toxicity in rats[J]
.Toxicology, 1996,107:39-45.), its concrete mechanism also needs to study further.Tissue pathological slice reflects Rhizoma Curcumae Longae
After extractum dissipates effect, liver organization pathological changes has obtained obvious improvement, proves that Liver of Ctenopharyngodon Idellus is repaired further.It addition,
Turmeric paste dissipates under effect, and each index reduces or strengthens degree and is more than positive controls, illustrates that turmeric paste dissipates action effect excellent
Dissipate in positive control medicine liver and gall Li Kang.
In sum, turmeric paste dissipates and has certain repair to fish liver damage, and application turmeric paste scatters
Send out fishing hepatoprotective medicine and there is good prospect.
2, turmeric paste dissipates big pool application test:
2.1 materials:
2.1.1 laboratory animal:
Turmeric paste dissipates the experimental subject of preventing liver injury: Wuhan City Gong Jia spreads three culturing pools of certain body raiser
The pool, the average 1.5m of the pond depth of water, it is 3 mu, main foster Ctenopharyngodon idellus.Number consecutively is No. 1, No. 2, No. 3.Choose No. 1 pond for blank
Matched group, No. 2, No. 3 ponds be test group.
Turmeric paste dissipate treatment hepatic injury experimental subject: the cultivating pool of three the individual raisers in thing lake, Wuhan City.
Number consecutively is No. 1, No. 2, No. 3.Wherein test pond area 12.7 mu, Ctenopharyngodon idellus about 1000 tail for No. 1.No. 2 test pond areas
11.4 mu, Ctenopharyngodon idellus about 1000 tail, No. 3 pond areas 20.5 mu, Ctenopharyngodon idellus about 2000 tail.No. 1 pond is set to matched group, No. 2 ponds
The pool is positive drug (GB Chinese medicine liver and gall Li Kang dissipate) group, and No. 3 ponds are that turmeric paste dissipates test group.
2.1.2 Experimental agents:
Trial drug curcumin extractum dissipates, content 1.349mg/g, and Hubei TCM Universities provides；Control drug liver and gall Li Kang
Dissipating, Wuhan Chopper Fishery Bio-Tech Co., Ltd. produces, product batch number 130304.
2.2 test methods and result:
2.2.1 prophylactic tria method:
Dissipating mix fodder with turmeric paste to throw something and feed test group pond, addition is the 1 ‰ of forage volume, and blank group is thrown something and fed sky
White commercial feed, 2 times on the 1st, 30d altogether.After off-test, 3 groups of tests, pond is each fishes for Ctenopharyngodon idellus 20 tail, anatomic observation liver at random
Dirty form.
2.2.2 therapeutic test method:
The Ctenopharyngodon idellus in 3 ponds selected by treatment all has disease condition, determines that Ctenopharyngodon idellus suffers from liver and gallbladder syndrome, and 3 through dissecting
Ctenopharyngodon idellus average extent in individual pond is suitable.The feedstuff of No. 2 test group adds positive drug liver and gall Li Kang and dissipates, and addition is feedstuff
The 2 ‰ of amount.The feedstuff of No. 3 test group adds turmeric paste and dissipates, and addition is that the 1 ‰ of forage volume, No. 1 blank group is thrown something and fed sky
White feedstuff.2 times on the 1st, throw something and feed 7d continuously.After off-test, 3 groups of tests, pond is each fishes for Ctenopharyngodon idellus 20 tail, anatomic observation at random
Liver morphology.
2.2.3 turmeric paste dissipates the result of the test of preventing liver injury:
After off-test, dissecting and find that blank group Liver of Ctenopharyngodon Idellus color is turned white, in anemia state, some liver is sent out
Huang, and test group Liver of Ctenopharyngodon Idellus uniform color, in cerise, flexible.
Turmeric paste dissipates and Ctenopharyngodon idellus protective rate reaches 97.5%, and the blank assay group grass carp liver not using turmeric paste to dissipate damages
The rate of wound reaches 95%, the results are shown in Table 3.
Table 3 turmeric paste dissipates preventing liver injury result of the test
2.2.4 turmeric paste dissipates and treats hepatic injury result of the test:
Dissect Ctenopharyngodon idellus before test, find liver turn white, turn to be yellow, frangible, after off-test, medication Liver of Ctenopharyngodon Idellus color is fresh
Red, flexible, and blank group, Liver of Ctenopharyngodon Idellus turns white, jaundice situation is serious.Turmeric paste dissipates the cure rate for the treatment of hepatic injury
Reaching 90%, the cure rate that positive drug liver and gall Li Kang dissipate reaches 85%, and the spontaneous recovery rate of blank assay group only has 5%, the results are shown in Table
4。
Table 4 turmeric paste dissipates treatment hepatic injury result of the test
3 discuss
In prophylactic tria, mixing and raise the 30d that throws something and feeds, in blank group, Ctenopharyngodon idellus has 95% hepatic injury occur, and test group is thrown something and fed and mixed
Occurring that the Ctenopharyngodon idellus of hepatic injury only has 2.5% after having the feedstuff that turmeric paste is scattered, prophylactic tria result understands, the Rhizoma Zingiberis Recens that this experiment is developed
Yellow extractum dissipates the effect with good hepatoprotective.In therapeutic test, turmeric paste dissipates the cure rate to grass carp liver damage and reaches
To 90%, and positive drug liver and gall Li Kang dissipate and the cure rate of hepatic injury are reached 85%, do not have naturally the fullying recover from an illness of blank group of medication
More rate only has 5%, and therapeutic test result understands, and turmeric paste dissipates and Fish hepatic injury is had good repair.
The test of the big pool proves, turmeric paste dissipates well prevention and repair to hepatic injury, can apply to Ctenopharyngodon idellus
Prevention and treatment to liver and gallbladder syndrome in breeding process.
Claims (1)
1. a preparation method for the Chinese medicine preparation of Ctenopharyngodon idellus hepatoprotective, the steps include:
A, take 50 mesh Rhizoma Curcumae Longae medicinal powder 50g, with 500 mL 80%v/v alcohol reflux 4 times, each 80 minutes；
B, filtration extracting solution, merging, be concentrated into 500 mL with Rotary Evaporators, it is thus achieved that Rhizoma Curcumae Longae extract；
C, being transferred in beaker by Rhizoma Curcumae Longae extract, 80 DEG C of water-baths volatilize to 250 mL, it is thus achieved that turmeric paste, add Semen Maydis and form sediment
Powder mix homogeneously；
D, it is placed in baking oven again, at 82 DEG C, is dried 9h；
E, taking-up dry drug cross No. 6 sieves, obtain the Chinese medicine preparation of hepatoprotective；
The Chinese medicine preparation of described Ctenopharyngodon idellus hepatoprotective is made up of the raw material of following weight portion:
Parts by weight of raw materials
Turmeric paste 5
Corn starch 8；
The Chinese medicine preparation of described hepatoprotective addition in feedstuff is 1 ‰, and the feeding volume of 5% is thrown something and fed 2 times, raise continuously every day
Feed 7 days.
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| CN117482197A (en) * | 2023-11-27 | 2024-02-02 | 中国热带农业科学院热带生物技术研究所 | Composition for preventing fatty liver of fish and shrimp, application and feed thereof |
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| 陶璐等.HPLC法测定姜黄浸膏散中姜黄素含量.《亚太传统医药》.2013,第9卷(第7期),24-26. * |
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