CN103289897A - Cell culture bottle - Google Patents
Cell culture bottle Download PDFInfo
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- CN103289897A CN103289897A CN2013102288216A CN201310228821A CN103289897A CN 103289897 A CN103289897 A CN 103289897A CN 2013102288216 A CN2013102288216 A CN 2013102288216A CN 201310228821 A CN201310228821 A CN 201310228821A CN 103289897 A CN103289897 A CN 103289897A
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- bottle
- tissue culture
- culture flask
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Abstract
The invention discloses a cell culture bottle, which belongs to the field of medical equipment. The cell culture bottle comprises a bottle body and a bottle cap, wherein the bottle cap is fixed at the bottle mouth of the bottle body, and an annular cell adherence medium layer is fixed inside the cavity of the bottle body, and is separated from the inner wall of the bottle body. The cell culture bottle has the following technical effects that as the cell adherence medium layer is additionally arranged in the cavity of the bottle body of the cell culture bottle, the superficial area for cell adherence culture inside the cell culture bottle is increased, and therefore, the density of the cell adhered to the inner wall of the cell culture bottle is increased and the once cell culture amount of the cell culture bottle is improved in a cell culture process.
Description
Technical field
The present invention relates to a kind of Tissue Culture Flask in medical equipment field.
Background technology
Tissue Culture Flask is the important equipment that carries out drug test and implantable medical devices test, and the stopping property of Tissue Culture Flask and security are quite high, thereby are subjected to pharmaceutical industries and medical device industry researchist's extensive favor.Tissue Culture Flask can be divided into according to material: glass Tissue Culture Flask and plastics Tissue Culture Flask, the shape of Tissue Culture Flask bottom can be circle, square, rectangle, trilateral etc.The volume of Tissue Culture Flask has plurality of specifications such as 500ml to 3L.The adherent area of Tissue Culture Flask namely can carry out the surface-area that cell attachment is cultivated in the Tissue Culture Flask, comprise 275cm
2～3000cm
2Plurality of specifications.
Yet present Tissue Culture Flask all exists a technical bottleneck, the amount that is a culturing cell of Tissue Culture Flask can't satisfy experimental requirements, therefore need more than one Tissue Culture Flask to carry out cell cultures, cause the increase of cost in medicine and the medicine equipment R﹠D process, the waste of resource.
Cause the reason of the problems referred to above to be: the amount that mainly improves a culturing cell of Tissue Culture Flask at present by the volume that increases Tissue Culture Flask.But this thinking can not improve the amount of a culturing cell of Tissue Culture Flask safely, and the sealing property of Tissue Culture Flask also may significantly reduce, and contaminated risk strengthens in the cell cultivation process.
Summary of the invention
The objective of the invention is provides a kind of Tissue Culture Flask in order to overcome the deficiencies in the prior art, and it can improve in the cell cultivation process, cell adherent density in Tissue Culture Flask, thus improve the amount of a culturing cell of Tissue Culture Flask.
A kind of technical scheme that realizes above-mentioned purpose is: a kind of Tissue Culture Flask, comprise bottle and bottle cap, and described bottle cap is fixed on the bottleneck of described bottle;
In the inner chamber of described bottle, the cell attachment medium layer of annular is arranged fixedly, described cell attachment medium layer separates with the inwall of described bottle.
Further, described cell attachment medium layer is the non-woven fabrics plicated layer.
Further again, the center of the intracavity bottom of described bottle is provided with projection, and described non-woven fabrics plicated layer is radially fixing in the inner chamber of described bottle.
Further again, the thickness of the employed non-woven fabrics of described non-woven fabrics plicated layer is 2～3mm, and is formed by the trevira braiding, and the diameter of described trevira is 20～30 μ m.
Further again, the vacuum plasma surface treatment is all passed through on the two sides of the inwall of described bottle and described non-woven fabrics plicated layer.
Further, described bottle cap is a kind of in closed cover, polyester lid, ventilating cover and the dottle pin lid.
Further, described bottle is made by glass.
Further again, described bottle is made by borosilicate glass.
Further, described bottle is made of plastics.
Further again, described bottle is made by polystyrene or polyester.
Adopted the technical scheme of a kind of Tissue Culture Flask of the present invention, namely in the inner chamber of the bottle of Tissue Culture Flask, the cell attachment medium layer of annular has fixedly been arranged, the technical scheme that described cell attachment medium layer separates with the inwall of described bottle.Its technique effect is: owing to increased the cell attachment medium layer in the inner chamber of the bottle of Tissue Culture Flask, increased and to have carried out the surface-area that cell attachment is cultivated in the Tissue Culture Flask, therefore, in the cell cultivation process, cell adherent density in Tissue Culture Flask increases, thereby improves the amount of a culturing cell of Tissue Culture Flask.
Description of drawings
Fig. 1 is the front view of a kind of Tissue Culture Flask of the present invention
Fig. 2 is that the A-A of Fig. 1 is to view.
Embodiment
See also Fig. 1 and Fig. 2, the present inventor be in order to understand technical scheme of the present invention better, below by embodiment particularly, and is described in detail by reference to the accompanying drawings:
See also Fig. 1, a kind of Tissue Culture Flask of the present invention comprises bottle 1 and bottle cap 2, and bottle cap 2 is fixed on the bottleneck 11 of bottle 1, to prevent that the cell in the Tissue Culture Flask from being polluted.
In the inner chamber 12 of bottle 1, the cell attachment medium layer 3 of annular is arranged fixedly, the bottom of the inner chamber 12 of the bottom of cell attachment medium layer 3 and bottle 1 is fixed, and cell attachment medium layer 3 separates with the inwall of bottle 1.
She Ji purpose is like this: the inner and outer wall of cell attachment medium layer 3 all can carry out cell attachment and cultivate, like this, the surface-area that can carry out the cell attachment cultivation in the Tissue Culture Flask increases to original three times at least, in the cell cultivation process, cell adherent density in Tissue Culture Flask increases to original three times, and the amount of a culturing cell of Tissue Culture Flask can increase to original three times.Therefore, the amount of a culturing cell of Tissue Culture Flask can satisfy experimental requirements, and cost reduces in medicine and the medicine equipment R﹠D process, and the loss of resource reduces.
In the present embodiment, in the inner chamber 12 of the bottle 1 of Tissue Culture Flask, what cell attachment medium layer 3 adopted is the non-woven fabrics plicated layer.The described non-woven fabrics plicated layer that cell attachment medium layer 3 adopts non-woven fabrics to make further increases can carry out the surface-area that cell attachment is cultivated in the Tissue Culture Flask.After adopting described non-woven fabrics plicated layer, the surface-area that can carry out the cell attachment cultivation in the Tissue Culture Flask increases to original five to seven times at least, in the cell cultivation process, cell adherent density in Tissue Culture Flask increases to original five to seven times, and the amount of a culturing cell of Tissue Culture Flask can increase to original five to seven times.The amount of a culturing cell of Tissue Culture Flask can further satisfy experimental requirements, and cost further reduces in medicine and the medicine equipment R﹠D process, and the loss of resource reduces.
Adopt the another one reason of described non-woven fabrics plicated layer to be: the perviousness of non-woven fabrics and permeability are all fine, can guarantee the inner and outer wall at described non-woven fabrics plicated layer, and the required nutriment of adherent cell growth and/or the supply of oxygen can both obtain well to satisfy on the inwall of bottle 1, and the surviving rate of cell in culturing process obtained significant raising.
In the present embodiment, described non-woven fabrics plicated layer preferred polyester fabric nonwoven cloth, because its cellular affinity is good, the preferred 2～3mm of the thickness of trevira non-woven fabrics, such thickness can both effectively be guaranteed described non-woven fabrics plicated layer structural stability, mechanical property, perviousness and ventilation property.Described trevira non-woven fabrics is to be that the trevira braiding of 20～30 μ m forms by diameter.Select that purpose of trevira is in this: described non-woven fabrics plicated layer structural stability, mechanical property, perviousness and ventilation property can both effectively be guaranteed.
In order to guarantee the structural stability of described non-woven fabrics plicated layer, the center of inner chamber 12 bottoms of bottle 1 is provided with projection 13, and described non-woven fabrics plicated layer is radially fixing in the inner chamber 12 of bottle 1.
The bottle cap 2 of Tissue Culture Flask mainly contains at present: closed cover, polyester lid, ventilating cover, dottle pin lid etc.
Interior pad is not with in the bottom of described closed cover, is used for the airtight cultivation of cell, guarantees the stopping property of Tissue Culture Flask in cell cultivation process.
Described polyester lid is usually used in open cell cultures, adopting Tissue Culture Flask to come in the process of culturing cell, only need unscrew described polyester lid gently, the interior gas of Tissue Culture Flask and the gas in the environment are exchanged, simultaneously, described polyester lid also is easy to fix with the described non-woven fabrics plicated layer that trevira is woven into.
The bottom of described ventilating cover has increased the hydrophobic filter membrane (not shown) of 0.2 μ m, in order to provide the aseptic gas exchange for carrying out cultured cells in the Tissue Culture Flask, reduces the contaminated risk of cell in the Tissue Culture Flask.Use the Tissue Culture Flask of described ventilating cover, be usually used in the open culture of cell, be particluarly suitable for CO
2Incubator carries out cell cultures, is particularly useful for carrying out the experiment of long-term cell cultures.
The bottom of described dottle pin lid has increased the dottle pin (not shown), the top of described dottle pin and described non-woven fabrics plicated layer is fixed, have the precut mouth on the described dottle pin, can make suction nozzle like this, perhaps the transfer pipet of the following specification of 5ml thrusts this dottle pin, Tissue Culture Flask is carried out the operation of liquid feeding, imbibition or harvested cell, reduce the contaminated chance of cell in the cell dispensing bottle, keep Tissue Culture Flask inner sealing sterile environment.
Consider that from the angle that cell attachment is cultivated the material of the bottle 1 of Tissue Culture Flask can be selected glass or plastics for use.In the present embodiment, bottle 1 preferred borosilicate glass, polystyrene or the polyester of Tissue Culture Flask.Borosilicate glass, polyester or polystyrene are good to the affinity of cell on the one hand, and the one side corrosion resistance and good is beneficial to cell in the cultivation of Tissue Culture Flask, especially long-term cultivation in addition.
Simultaneously, the inwall of bottle 1, and the inner and outer wall of described non-woven fabrics plicated layer all can be handled by the special surface modification, the inwall of the further bottle 1 that improves, and the cellular affinity of the inner and outer wall of described non-woven fabrics plicated layer, be convenient to carry out more cellulous adherent culture, simultaneously cell the two sides of the inwall of bottle 1 and described non-woven fabrics plicated layer breed with adherent after, relatively easily digest.Described special surface modification is handled and is comprised vacuum plasma surface treatment etc.
Claims (10)
1. a Tissue Culture Flask comprises bottle (1) and bottle cap (2), and described bottle cap (2) is fixed on the bottleneck (11) of described bottle (1), it is characterized in that:
In the inner chamber (12) of described bottle (1), the cell attachment medium layer (3) of annular is arranged fixedly, described cell attachment medium layer (3) separates with the inwall of described bottle (1).
2. a kind of Tissue Culture Flask according to claim 1, it is characterized in that: described cell attachment medium layer (3) is the non-woven fabrics plicated layer.
3. a kind of Tissue Culture Flask according to claim 2 is characterized in that: the center of inner chamber (12) bottom of described bottle (1) is provided with projection (13), and described non-woven fabrics plicated layer is radially fixing in the inner chamber (12) of described bottle (1).
4. according to any described a kind of Tissue Culture Flask in the claim 1 to 3, it is characterized in that: a kind of in closed cover, polyester lid, ventilating cover and the dottle pin lid of described bottle cap (2).
5. according to any described a kind of Tissue Culture Flask in the claim 1 to 3, it is characterized in that: described bottle (1) is made by glass.
6. a kind of Tissue Culture Flask according to claim 5, it is characterized in that: described bottle (1) is made by borosilicate glass.
7. according to any described a kind of Tissue Culture Flask in the claim 1 to 3, it is characterized in that: described bottle (1) is made of plastics.
8. a kind of Tissue Culture Flask according to claim 7, it is characterized in that: described bottle (1) is made by polystyrene or polyester.
9. according to claim 2 or 3 described a kind of Tissue Culture Flasks, it is characterized in that: the thickness of the employed non-woven fabrics of described non-woven fabrics plicated layer is 2～3mm, and is formed by the trevira braiding, and the diameter of described trevira is 20～30 μ m.
10. according to claim 2 or 3 described a kind of Tissue Culture Flasks, it is characterized in that: the vacuum plasma surface treatment is all passed through on the inwall of described bottle (1) and the two sides of described non-woven fabrics plicated layer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310228821.6A CN103289897B (en) | 2013-06-08 | 2013-06-08 | Cell culture bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310228821.6A CN103289897B (en) | 2013-06-08 | 2013-06-08 | Cell culture bottle |
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| Publication Number | Publication Date |
|---|---|
| CN103289897A true CN103289897A (en) | 2013-09-11 |
| CN103289897B CN103289897B (en) | 2014-11-19 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310228821.6A Active CN103289897B (en) | 2013-06-08 | 2013-06-08 | Cell culture bottle |
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| Country | Link |
|---|---|
| CN (1) | CN103289897B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108315258A (en) * | 2018-05-10 | 2018-07-24 | 章毅 | Increase cell culture roller bottle and its application of cell culture area |
| CN109797102A (en) * | 2017-11-17 | 2019-05-24 | 北京中原合聚经贸有限公司 | A kind of adherent uniformity device of control microcarrier cell inoculation |
| CN114250147A (en) * | 2021-12-29 | 2022-03-29 | 上海日泰医药设备工程有限公司 | Biological reaction device |
| CN114276927A (en) * | 2021-12-29 | 2022-04-05 | 上海日泰医药设备工程有限公司 | Folding carrier column for bioreactor |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020155594A1 (en) * | 2001-03-27 | 2002-10-24 | Hsieh Helen V. | Method and apparatus for culturing cells |
| US7247471B2 (en) * | 2003-01-02 | 2007-07-24 | Becton, Dickinson And Company | Spiral pleated roller bottle |
| US20110281343A1 (en) * | 2008-10-02 | 2011-11-17 | Gay Roger J | Bioreactor with rods arrayed for culturing anchorage-dependent cells |
| CN102409020A (en) * | 2010-09-26 | 2012-04-11 | 上海泰因生物技术有限公司 | Non woven/polyester fiber carriers for culturing cells and use method thereof |
| CN203403093U (en) * | 2013-06-08 | 2014-01-22 | 上海日泰医药设备工程有限公司 | Cell culture bottle |
-
2013
- 2013-06-08 CN CN201310228821.6A patent/CN103289897B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020155594A1 (en) * | 2001-03-27 | 2002-10-24 | Hsieh Helen V. | Method and apparatus for culturing cells |
| US7247471B2 (en) * | 2003-01-02 | 2007-07-24 | Becton, Dickinson And Company | Spiral pleated roller bottle |
| US20110281343A1 (en) * | 2008-10-02 | 2011-11-17 | Gay Roger J | Bioreactor with rods arrayed for culturing anchorage-dependent cells |
| CN102409020A (en) * | 2010-09-26 | 2012-04-11 | 上海泰因生物技术有限公司 | Non woven/polyester fiber carriers for culturing cells and use method thereof |
| CN203403093U (en) * | 2013-06-08 | 2014-01-22 | 上海日泰医药设备工程有限公司 | Cell culture bottle |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109797102A (en) * | 2017-11-17 | 2019-05-24 | 北京中原合聚经贸有限公司 | A kind of adherent uniformity device of control microcarrier cell inoculation |
| CN108315258A (en) * | 2018-05-10 | 2018-07-24 | 章毅 | Increase cell culture roller bottle and its application of cell culture area |
| CN114250147A (en) * | 2021-12-29 | 2022-03-29 | 上海日泰医药设备工程有限公司 | Biological reaction device |
| CN114276927A (en) * | 2021-12-29 | 2022-04-05 | 上海日泰医药设备工程有限公司 | Folding carrier column for bioreactor |
| CN114276927B (en) * | 2021-12-29 | 2024-05-10 | 上海日泰医药设备工程有限公司 | Folding carrier column for bioreactor |
| CN114250147B (en) * | 2021-12-29 | 2024-05-14 | 上海日泰医药设备工程有限公司 | Biological reaction device |
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| Publication number | Publication date |
|---|---|
| CN103289897B (en) | 2014-11-19 |
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