CN216712123U - Ventilative digestion culture dish - Google Patents

Abstract

The application discloses ventilative digestion culture dish relates to the medical and hygienic apparatus. This ventilative digestion culture dish includes culture dish body, filter screen and culture dish lid, the air vent has been seted up to the top outer lane of culture dish lid, be provided with aseptic filter membrane in the air vent, the top inner circle of culture dish lid is connected with perspective layer, culture dish lid with culture dish body swing joint, the filter screen can dismantle connect in the bottom of culture dish body. The utility model can visually observe the tissue digestion and cell growth conditions in the culture dish through the perspective layer, and the sterile filter membrane can effectively prevent cross infection, is easy to ventilate and is convenient to mark.

Description

Ventilative digestion culture dish

Technical Field

The application relates to the technical field of sanitary medical equipment, in particular to a breathable digestion culture dish.

Background

In vitro culture of cells and tissues has become an indispensable matter for life research and practice, and in the fields of biology, medicine and the like, various cells need to be cultured in the processes of research, disease prevention, treatment and the like, and different cell culture environments have different requirements, and equipment for cell culture also has different requirements, including culture dishes, culture dishes and the like. The culture dish is the most common laboratory vessel in molecular biology experiments, consists of a circular disc-shaped bottom and a cover, and has the advantages of simple structure, single function and defects.

If the gap between the circular disc-shaped bottom of the culture dish and the culture dish cover is large, the sterile environment cannot be ensured, and cell pollution is easily caused; if the gap between the circular disc-shaped bottom of the culture dish and the culture dish cover is small, oxygen circulation cannot be guaranteed, air in the culture dish is difficult to flow, and finally cells die; in addition, when the tissue is digested, the tissue mass must be blown to be sufficiently digested, and during the cell culture, the liquid must be changed periodically, but the pipette is easily clogged by the tissue mass of the cells.

SUMMERY OF THE UTILITY MODEL

Therefore, the application provides a ventilative digestion culture dish to solve the unable audio-visual tissue digestion and the cell growth situation in the observation culture dish of current culture dish, and cross infection easily, be difficult to take a breath, the problem of the mark of being not convenient for.

In order to achieve the above purpose, the present application provides the following technical solutions:

the utility model provides a ventilative digestion culture dish, includes culture dish body, filter screen and culture dish lid, the air vent has been seted up to the top outer lane of culture dish lid, be provided with aseptic filter membrane in the air vent, the top inner circle of culture dish lid is connected with perspective layer, culture dish lid with culture dish body swing joint, the filter screen can dismantle connect in the bottom of culture dish body.

Optionally, the culture dish is made of polypropylene or polystyrene or borosilicate material.

Optionally, the sterile filter is a 0.22 μm hydrophobic filter.

Optionally, the inner wall of the culture dish cover is provided with an internal thread, the outer wall of the culture dish body is provided with an external thread matched with the internal thread, and the culture dish cover is in threaded connection with the culture dish body.

Optionally, a handle is detachably connected to the filter net.

Optionally, the vent hole occupies 2/3 of the top area of the culture dish cover, and the see-through layer occupies 1/3 of the top surface of the culture dish cover.

Optionally. And a label is arranged on the perspective layer.

Compared with the prior art, the method has the following beneficial effects:

because the digestion culture bottle adopts the high polymer material polypropylene or polystyrene and the 0.22 mu m hydrophobic filter membrane, the requirement of the cells on gas exchange in the digestion and culture processes is met, the cell pollution is effectively prevented, the survival rate of the cells is improved, and meanwhile, the culture dish cover is provided with the perspective layer, the appearance is smooth, and the transparency is high, so that the tissue digestion and cell growth conditions can be observed in the digestion and culture processes. The phenomenon that cells cultured in a culture dish are easy to pollute is solved, and meanwhile, the digested tissue blocks can be filtered, so that the pipette is prevented from being blocked, and the test efficiency is improved.

Drawings

To more intuitively illustrate the prior art and the present application, several exemplary drawings are given below. It should be understood that the specific shapes, configurations and illustrations in the drawings are not to be construed as limiting, in general, the practice of the present application; for example, it is within the ability of those skilled in the art to make routine adjustments or further optimizations based on the technical concepts disclosed in the present application and the exemplary drawings, for the increase/decrease/attribution of certain units (components), specific shapes, positional relationships, connection manners, dimensional ratios, and the like.

FIG. 1 is a schematic overall structure diagram of one embodiment of the present application;

FIG. 2 is a schematic illustration of an explosive structure according to an embodiment of the present application;

FIG. 3 is a schematic view of a culture dish lid according to an embodiment of the present application;

FIG. 4 is a schematic view of a culture dish body according to an embodiment of the present application;

fig. 5 is a schematic structural diagram of a filter screen according to an embodiment of the present application.

Description of reference numerals:

1. a culture dish body; 2. a filter screen; 3. a culture dish cover; 4. a vent hole; 5. a sterile filtration membrane; 6. a see-through layer; 7. a label; 8. a handle.

Detailed Description

The present application will be described in further detail below with reference to specific embodiments thereof, with reference to the accompanying drawings.

In the description of the present application: "plurality" means two or more unless otherwise specified. The terms "comprising," "including," "having," and the like, as well as "comprising" (and variations thereof) are intended to mean "including, but not limited to" (some elements, components, materials, steps, etc.).

In the present application, terms such as "upper", "lower", "left", "right", "middle", and the like are usually used for easy visual understanding with reference to the drawings, and are not intended to be an absolute limitation of the positional relationship in an actual product. Changes in these relative positional relationships are also considered to be within the scope of the present disclosure without departing from the technical concepts disclosed in the present disclosure.

As shown in fig. 1-5, a permeable digestion culture dish comprises a culture dish body 1, a filter screen 2 and a culture dish cover 3. The diameter of the culture dish cover 3 is 9cm, the height of the culture dish cover is 2cm, the outer ring of the top of the culture dish cover is connected with a vent hole 4, the area of the vent hole 4 is 2/3 which accounts for the area of the top of the culture dish cover 3, a sterile filter membrane 5 is arranged in the vent hole 4, and the sterile filter membrane 5 is a 0.22 mu m hydrophobic filter membrane. The top inner circle of culture dish lid 3 is connected with perspective layer 6, and the area of perspective layer 6 is for accounting for its place culture dish lid 3 top area 1/3, is provided with label 7 on the perspective layer 6, and label 7 sets up in culture dish lid 3 perspective layer 6 upper end, is the rectangle shape, and culture dish lid 3 is provided with has the internal thread. Filter screen 2 demountable installation is in the culture dish bottom, and filter screen 2 is equipped with handle 8, and 1 diameter 9cm of culture dish body is highly 3cm, and culture dish body 1 is provided with and has the external screw thread. The culture dish is made of high polymer material polypropylene (PP) or Polystyrene (PS) or borosilicate material, the inner surfaces of the bottle body and the bottle cover are smooth, the transparency is high, the digestion condition of tissues and the growth condition of cells can be directly observed under the condition that the culture dish is not opened in the digestion culture process, and the high-pressure sterilization and the reutilization can be realized. 3 outer lanes of culture dish lid set up air vent 4, more are favorable to the air to flow, guarantee that the inside air feed of bottle is sufficient, set up 0.22 mu m hydrophobic filter membrane in air vent 4 department, again can effectually prevent to pollute to gas exchange's demand in satisfying cell culture. The culture dish body 1 is provided with an external thread, the culture dish cover 3 is provided with an internal thread, and the external thread is matched with the internal thread to seal the culture dish. The culture dish body 1 is provided with demountable installation to culture dish bottom filter screen 2, and it is fast to filter the tissue of having digested, avoids blockking up the pipette.

The filter screen 2 is provided with the handle 8 of convenient dismantlement installation, and the dismantled connection of handle 8 and filter screen 2 is threaded connection, sets up threaded hole on filter screen 2, and one side fixedly connected with that handle 8 and filter screen 2 are connected is with the bolt of screw hole looks adaptation to the convenience is carried out the dismouting to handle 8.

In the utility model, after the tissue is digested, a tester can filter the cell tissue block by disassembling the filter screen 2, the external thread of the culture dish body 1 is matched with the internal thread of the culture dish cover 3 to seal the culture dish, so that the cell pollution is prevented, and the air holes of the culture dish cover 3 can meet the requirement of gas exchange in cell culture.

In fig. 1, the upper part of the digestion culture dish is provided with an openable and sealed culture dish cover 3, the lower part of the digestion culture dish is provided with an openable and sealed culture dish body 1, and a filter screen 2 which is detachably arranged at the bottom of the culture dish is arranged inside the culture dish body 1.

In fig. 2, for the decomposition of digestion culture dish sketch map, digestion culture dish lid 3 accessible culture dish lid 3 sets up external screw thread cooperation and seals the digestion culture dish on internal thread and the culture dish body 1, and filter screen 2 is provided with the handle 8 of convenient dismantlement installation, installs to culture dish body 1 bottom in the cell digestion, and with pancreatin full contact digestion, through demolising filter screen 2, filter the tissue piece after the tissue digestion finishes.

In fig. 3, be equipped with air vent 4 on the digestion culture dish lid 3, more be favorable to the air to flow, guarantee that the inside air feed of bottle is sufficient, set up 0.22 mu m hydrophobic filter membrane in air vent 4 department, to the demand of gas exchange in satisfying the cell culture, again can effectually prevent to pollute, digestion culture dish lid 3 central part has the perspective layer 6 of diameter 2cm, and perspective layer 6 upper end is equipped with rectangle label 7, and culture dish lid 3 sets up the internal thread.

In fig. 5, the filter screen 2 is provided with a handle 8 which is convenient to detach and mount.

In fig. 4, the culture dish body 13 is provided with an external thread, and the external thread and the internal thread are matched to seal the culture dish.

When in manufacturing, the diameter of the culture dish cover 3 of the digestion culture dish is 9cm, and the height is 3 cm; the top outer ring of the culture dish is connected with a vent hole 4, the area of the vent hole is 2/3 occupying the top area of the culture dish cover 3, a sterile filter membrane 5 is arranged in the vent hole 4, and the sterile filter membrane 5 is a 0.22 mu m hydrophobic filter membrane, so that the requirement of gas exchange is met. The top inner circle of culture dish lid 3 is connected with perspective layer 6, the area of perspective layer 6 is 1/3 for occupying its place culture dish lid 3 top area, be provided with label 7 on the perspective layer 6, culture dish lid 3 is provided with and has the internal thread. Filter screen 2 demountable installation is to the culture dish bottom, filter screen 2 is equipped with handle 8, 1 diameter 90cm of culture dish body, highly be 3cm, culture dish body 1 sets up by having the external screw thread, and culture dish body 1 external screw thread seals the culture dish with 3 interior screw-thread fit of culture dish lid, prevents cell contamination.

The culture dish body 1 and the culture dish cover 3 are made of high polymer materials such as polypropylene (PP) or Polystyrene (PS) or borosilicate materials, the inner surfaces of the bottle body and the bottle cover are smooth, the transparency is high, the digestion condition of tissues and the growth condition of cells can be directly observed under the condition that the culture dish is not opened in the digestion culture process, and the high-pressure sterilization and the recycling can be realized.

All the technical features of the above embodiments can be arbitrarily combined (as long as there is no contradiction between the combinations of the technical features), and for brevity of description, all the possible combinations of the technical features in the above embodiments are not described; these examples, which are not explicitly described, should be considered to be within the scope of the present description.

The present application has been described in considerable detail with reference to certain embodiments and examples thereof. It should be understood that several general adaptations or further innovations of these specific embodiments can also be made based on the technical idea of the present application; however, such conventional modifications and further innovations may also fall within the scope of the claims of the present application as long as they do not depart from the technical idea of the present application.

Claims (7)

1. A ventilated digestion culture dish, characterized in that: including culture dish body (1), filter screen (2) and culture dish lid (3), air vent (4) have been seted up to the top outer lane of culture dish lid (3), be provided with aseptic filter membrane (5) in air vent (4), the top inner circle of culture dish lid (3) is connected with perspective layer (6), culture dish lid (3) with culture dish body (1) swing joint, filter screen (2) can dismantle connect in the bottom of culture dish body (1).

2. A gas permeable digestion vessel in accordance with claim 1, wherein: the culture dish is made of polypropylene or polystyrene or borosilicate material.

3. A gas permeable digestion vessel according to claim 1, wherein: the sterile filter membrane (5) is a 0.22 mu m hydrophobic filter membrane.

4. A gas permeable digestion vessel according to claim 1, wherein: the internal thread is seted up to the inside wall of culture dish lid (3), the lateral wall of culture dish body (1) seted up with the external screw thread of internal thread looks adaptation, culture dish lid (3) with culture dish body (1) threaded connection.

5. A gas permeable digestion vessel according to claim 1, wherein: the filter screen (2) is detachably connected with a handle (8).

6. A gas permeable digestion vessel according to claim 1, wherein: the vent holes (4) account for 2/3 in the area of the top of the culture dish cover (3), and the perspective layer (6) accounts for 1/3 in face-to-face connection with the top of the culture dish cover (3).

7. A gas permeable digestion vessel in accordance with claim 1, wherein: and a label (7) is arranged on the perspective layer.

Images