CN204174219U - A kind of cell in vitro sterile culture device - Google Patents

A kind of cell in vitro sterile culture device Download PDF

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Publication number
CN204174219U
CN204174219U CN201420661401.7U CN201420661401U CN204174219U CN 204174219 U CN204174219 U CN 204174219U CN 201420661401 U CN201420661401 U CN 201420661401U CN 204174219 U CN204174219 U CN 204174219U
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China
Prior art keywords
plate
square
coaming plate
cell
inside casing
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Expired - Fee Related
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CN201420661401.7U
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Chinese (zh)
Inventor
师岩
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Qiqihar Medical University
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Qiqihar Medical University
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Abstract

The utility model discloses a kind of cell in vitro sterile culture device, be made up of upper cover assembly, box assembly, hinge, square water box, square interior box assembly, square year cell container frame component.Two upper cover assemblies can be connected with box assembly respectively by hinge in folding, during connection, two opening surroundings of two opening cover plate are tightly connected the back-shaped gasket of two upper cover assembly lower ends with the band on box assembly respectively, in left and right two inner chambers be separated into by two middle risers in the inside casing coaming plate of box assembly respectively by toroidal membrane, lower toroidal membrane, matrix pattern dividing plate be connected with square interior box assembly, in square interior box assembly, be placed with square water box and be connected with square year cell container frame component by its support plate.Adopt left and right two square interior box assemblies can when long period cell cultures, cell culture incubator be used alternatingly, UV-lamp sterilization, the situation that can cell bacterial avoided completely to pollute, and is the comparatively simple and economical and practical Cell culture invitro device of a kind of structure.

Description

A kind of cell in vitro sterile culture device
Technical field
The utility model relates to a kind of cell in vitro sterile culture device, belongs to health care equipment technical field.
Background technology
Cell cultures is one of research method important in medical research experiment, and to temperature, humidity, gas concentration lwevel in cell cultivation process, particularly sterility requires very high, therefore requires higher to cell culture apparatus.General import cell culture apparatus is expensive, and be unfavorable for that middle-size and small-size laboratory is popularized, domestic device mostly adopts external heating mode, only has single box structure, and when the cell cultures time is longer, cell is easy to case internal contamination, breed bacteria, and causes cell cultures failure.
Summary of the invention
In order to solve the problem, the purpose of this utility model is to provide a kind of cell in vitro sterile culture device, left and right two inner chambers are provided with, the carbonic acid gas leaving air control valve, ceramic heating plate, the UV-lamp that the identical square interior box assembly of two covers and square water box are housed and are independently controlled separately by gas concentration lwevel controller, temperature regulator, device for control ultra violet lamp in device.Adopt five faces all with heating in ceramic heating plate, there is quick heating, respond fast feature, big uncork square water box is adopted to have enough steam emission, need not control to meet humidity requirement, adopt left and right two interior casees, case in all can using as culturing cell, and can sterilizing in case separately.When needs long period cell cultures, can first cultivate in left case, after certain hour, square year cell container frame component is transferred to rapidly in right case carry out cell cultures together with being carried a cell container, ultraviolet lamp sterilizing is used in advance in right case, ensure that continuous sterile culture, and ultraviolet lamp does not affect on cultured cells.After migrating out cell in left case, then open UV-lamp sterilizing, standby rotation uses, UV-lamp sterilizing is replaced for two casees in such certain hour, both can not affect cell normal growth, and can avoid again the cell contamination occurred because of long-time cell cultures completely, the situations such as breed bacteria occur.
The utility model solves the technical scheme that its technical problem adopts: a kind of cell in vitro sterile culture device, it is made up of upper cover assembly, box assembly, hinge, square water box, square interior box assembly, square year cell container frame component.Two upper cover assemblies can be connected with box assembly respectively by hinge in folding, during connection, two opening surroundings of two opening cover plate are tightly connected the back-shaped gasket of two upper cover assembly lower ends with the band on box assembly respectively, in left and right two inner chambers be separated into by two middle risers in the inside casing coaming plate of box assembly respectively by toroidal membrane, lower toroidal membrane, matrix pattern dividing plate be connected with square interior box assembly, in square interior box assembly, be placed with square water box and be connected with square year cell container frame component by its support plate.Described upper cover assembly is made up of outer spill frid, concave shaped frid, back-shaped banded plate, back-shaped gasket, U-shaped handle, UV-lamp, the following peripheral of outer spill frid is connected by the following peripheral of back-shaped banded plate with concave shaped frid, adiabator layer is connected with outside between spill frid and concave shaped frid, back-shaped banded plate is connected with back-shaped gasket, be fixed with U-shaped handle before spill frid outside, have UV-lamp at concave shaped frid internal fixtion.Described box assembly is by housing coaming plate, inside casing coaming plate, left side vertical plate, right side riser, middle plate, the two opening cover plate of band, base plate, bearing, upper toroidal membrane, lower toroidal membrane, matrix pattern dividing plate, bottom surface dividing plate, frame plate, glass, gas concentration lwevel controller, temperature regulator, device for control ultra violet lamp, rectangle ceramic heating plate, square ceramic hot-plate, dense carbon dioxide sensor, temperature sensor, carbon dioxide nozzle assembly is formed, and is placed with inside casing coaming plate, between housing coaming plate and inside casing coaming plate, is connected to left side vertical plate in housing coaming plate, right side riser, is connected with two middle risers, between two middle risers, is connected with adiabator layer in the middle part of inside casing coaming plate, in inside casing coaming plate periphery by housing coaming plate, left side vertical plate, right side riser is connected with adiabator layer, is connected with base plate, below base plate, is connected with bearing, the left side be separated into by two middle risers in inside casing coaming plate below housing coaming plate and inside casing coaming plate, be connected to bottom surface dividing plate below in right two inner chambers, in the space that bottom surface dividing plate and base plate are formed, be connected with adiabator layer, on housing coaming plate and inside casing coaming plate, be connected with the two opening cover plate of band, be with two openings on pair opening cover plate respectively with a left side, right two inner chambers are corresponding.Before housing coaming plate and inside casing coaming plate, first half correspondence is provided with opened front, is connected with frame plate, in frame plate, is connected with double glazing in two opened front inner circumferential.Toroidal membrane, lower toroidal membrane is all horizontally connected with in left and right two inner chambers, on inside casing coaming plate between upper toroidal membrane and lower toroidal membrane and inside middle riser left and right, be fixed with rectangle ceramic heating plate respectively below, inside casing coaming plate between lower toroidal membrane and bottom surface dividing plate and the face, front, rear, left and right inside middle riser are fixed with rectangle ceramic heating plate respectively, on the dividing plate of bottom surface, be connected with matrix pattern dividing plate, in its matrix pattern dividing plate, be fixed with square ceramic hot-plate respectively.Two carbon dioxide nozzle assemblies are sealedly connected with by inside casing coaming plate and adiabator layer after housing coaming plate, carbon dioxide nozzle assembly the inner puts in a left side respectively, in right two inner chambers, its outer end is connected with carbonic acid gas leaving air control valve respectively by pipeline, on a left side, both sides in right two inner chambers are fixed with dense carbon dioxide sensor respectively, temperature sensor, before housing coaming plate, both sides are connected to gas concentration lwevel controller from top to bottom, temperature regulator, device for control ultra violet lamp, left at it, the gas concentration lwevel controller on right both sides respectively with a left side, dense carbon dioxide sensor and the corresponding carbonic acid gas leaving air control valve of right two inner chambers connect, left at it, the temperature regulator on right both sides respectively with a left side, the temperature sensor of right two inner chambers, rectangle ceramic heating plate, square ceramic hot-plate connects, left at it, the device for control ultra violet lamp on right both sides respectively with a left side, the UV-lamp of right inside spill frid internal fixtion connects.Described square interior box assembly is made up of interior box main body, support plate, transparent panel, interior box main body is square structure, its upper end is the opening of inside flanging, two sides in interior box main body and middle and upper part are below connected with support plate, before interior box main body middle and upper part opening on be connected with transparent panel, the position of its opening and the first half before housing coaming plate and inside casing coaming plate be corresponding, and to be provided with opened front corresponding.Square year described cell container frame component is made up of upper frame plate, lower frame plate, square pillar, a year cell container, upper frame plate is connected with lower frame plate by four square pillars, upper frame plate is provided with container hole, corresponding container hole is provided with container bottom outlet on lower frame plate, lower frame plate is also provided with multiple through hole, be placed with in the container hole of upper frame plate and carry cell container, carry cell container lower end and be connected with container bottom outlet.
The beneficial effects of the utility model are: adopt left and right two square interior box assemblies, all can use as cell culture incubator, interior case is provided with UV-lamp, use after irradiating certain hour by UV-lamp control before using, when needs long period cell cultures, can first cultivate in left case, transfer to after certain hour in right case and proceed to cultivate, be used alternatingly UV-lamp sterilization, the situation that can cell bacterial avoided completely to pollute, can effectively play sterilization sterilization functions.Casing and square inner container assembly are provided with observation transparent glass, are the comparatively simple and economical and practical cell in vitro sterile culture devices of a kind of structure.
Accompanying drawing explanation
Below in conjunction with the drawings and specific embodiments, the utility model is described further.
Fig. 1 is the structural representation of a kind of cell in vitro sterile culture of the utility model device.
Fig. 2 is the sectional structure schematic diagram of Fig. 1.
Fig. 3 is the plan structure schematic diagram of Fig. 1.
Fig. 4 is A-A sectional structure schematic diagram of Fig. 2.
Fig. 5 is B-B sectional structure schematic diagram of Fig. 2.
Fig. 6 is C-C sectional structure schematic diagram of Fig. 2.
Fig. 7 is the square interior box assembly structural representation of Fig. 1.
Fig. 8 is the plan structure schematic diagram of Fig. 7.
Fig. 9 is the square year cell container frame component structural representation of Fig. 1.
Figure 10 is the plan structure schematic diagram of Fig. 9.
Figure 11 is D-D sectional structure schematic diagram of Fig. 9.
Reference numeral
1, upper cover assembly 2, box assembly 3, hinge 4, square water box 5, square interior box assembly
6, square year cell container frame component
11, outer spill frid 12, concave shaped frid 13, back-shaped banded plate 14, back-shaped gasket
15, U-shaped handle 16, UV-lamp
21, housing coaming plate 22, inside casing coaming plate 23, left side vertical plate 24, right side riser
25, middle riser 26, band two opening cover plate 27, base plate 28, bearing 29, upper toroidal membrane 30, lower toroidal membrane 31, matrix pattern dividing plate 32, bottom surface dividing plate 33, frame plate
34, glass 35, gas concentration lwevel controller 36, temperature regulator
37, device for control ultra violet lamp 38, rectangle ceramic heating plate 39, square ceramic hot-plate
40, gas concentration lwevel sensor 41, temperature sensor 42, carbon dioxide nozzle assembly
51, interior box main body 52, support plate 53, transparent panel
61, upper frame plate 62, lower frame plate 63, square pillar 64, container hole 65, container bottom outlet
66, through hole 67, year cell container.
Embodiment
Refer to Fig. 1 to Figure 11, a kind of cell in vitro sterile culture device, it is made up of upper cover assembly, box assembly, hinge, square water box, square interior box assembly, square year cell container frame component.Two upper cover assemblies 1 can be connected with box assembly 2 respectively by hinge 3 in folding, during connection, two opening surroundings of two opening cover plate 26 are tightly connected the back-shaped gasket 14 of two upper cover assembly 1 lower ends with the band on box assembly 2 respectively, the left side be separated into by two middle risers 25 in the inside casing coaming plate 22 of box assembly 2, respectively by upper toroidal membrane 29 in right two inner chambers, lower toroidal membrane 30, matrix pattern dividing plate 31 is connected with square interior box assembly 5, in square interior box assembly 5, be placed with square water box 4 and be connected with square year cell container frame component 6 by its support plate 52.Described upper cover assembly 1 is made up of outer spill frid 11, concave shaped frid 12, back-shaped banded plate 13, back-shaped gasket 14, U-shaped handle 15, UV-lamp 16, the following peripheral of outer spill frid 11 is connected with the following peripheral of concave shaped frid 12 by back-shaped banded plate 13, adiabator layer is connected with outside between spill frid 11 and concave shaped frid 12, back-shaped banded plate 13 is connected with back-shaped gasket 14, spill frid 11 is fixed with U-shaped handle 15 above outside, has UV-lamp 16 at concave shaped frid 12 internal fixtion.Described box assembly 2 is by housing coaming plate 21, inside casing coaming plate 22, left side vertical plate 23, right side riser 24, middle plate 25, the two opening cover plate 26 of band, base plate 27, bearing 28, upper toroidal membrane 29, lower toroidal membrane 30, matrix pattern dividing plate 31, bottom surface dividing plate 32, frame plate 33, glass 34, gas concentration lwevel controller 35, temperature regulator 36, device for control ultra violet lamp 37, rectangle ceramic heating plate 38, square ceramic hot-plate 39, dense carbon dioxide sensor 40, temperature sensor 41, carbon dioxide nozzle assembly 42 is formed, and is placed with inside casing coaming plate 22 in housing coaming plate 21, is connected to left side vertical plate 23 between housing coaming plate 21 and inside casing coaming plate 22, right side riser 24, is connected with two middle risers 25, between two middle risers 25, is connected with adiabator layer in the middle part of inside casing coaming plate 22, in inside casing coaming plate 22 periphery by housing coaming plate 21, left side vertical plate 23, right side riser 24 is connected with adiabator layer, is connected with base plate 27, is connected with bearing 28 below base plate 27, the left side be separated into by two middle risers 25 in inside casing coaming plate 22 below housing coaming plate 21 and inside casing coaming plate 22, bottom surface dividing plate 32 is connected to below in right two inner chambers, adiabator layer is connected with in the space that bottom surface dividing plate 32 and base plate 27 are formed, on housing coaming plate 21 and inside casing coaming plate 22, be connected with the two opening cover plate 26 of band, two openings on the two opening cover plate 26 of band respectively with a left side, right two inner chambers are corresponding.Before housing coaming plate 21 and inside casing coaming plate 22, first half correspondence is provided with opened front, is connected with frame plate 33, is connected with double glazing 34 in frame plate 33 in two opened front inner circumferential.On a left side, toroidal membrane 29 is all horizontally connected with in right two inner chambers, lower toroidal membrane 30, a left side on inside casing coaming plate 22 between upper toroidal membrane 29 and lower toroidal membrane 30 and inside middle riser 25, right, be fixed with rectangle ceramic heating plate 38 respectively below, before inside inside casing coaming plate 22 between lower toroidal membrane 30 and bottom surface dividing plate 32 and middle riser 25, after, left, the right side is fixed with rectangle ceramic heating plate 38 respectively, matrix pattern dividing plate 31 is connected with on bottom surface dividing plate 32, square ceramic hot-plate 39 is fixed with respectively in its matrix pattern dividing plate 31.At housing coaming plate 21 below and be sealedly connected with two carbon dioxide nozzle assemblies 42 by inside casing coaming plate 22 and adiabator layer, carbon dioxide nozzle assembly 42 the inner puts in a left side respectively, in right two inner chambers, its outer end is connected with carbonic acid gas leaving air control valve, on a left side respectively by pipeline, both sides in right two inner chambers are fixed with dense carbon dioxide sensor 40 respectively, temperature sensor 41, is connected to gas concentration lwevel controller 35 from top to bottom in both sides above at housing coaming plate 21, temperature regulator 36, device for control ultra violet lamp 37, left at it, the gas concentration lwevel controller 35 on right both sides respectively with a left side, dense carbon dioxide sensor 40 and the corresponding carbonic acid gas leaving air control valve of right two inner chambers connect, left at it, the temperature regulator 36 on right both sides respectively with a left side, the temperature sensor 41 of right two inner chambers, rectangle ceramic heating plate 38, square ceramic hot-plate 39 connects, left at it, the device for control ultra violet lamp 37 on right both sides respectively with a left side, the UV-lamp 16 of right inside spill frid 12 internal fixtion connects.Described square interior box assembly 5 is made up of interior box main body 51, support plate 52, transparent panel 53, interior box main body 51 is square structure, its upper end is the opening of inside flanging, two sides in interior box main body 51 and middle and upper part are below connected with support plate 52, the opening of interior box main body 51 above middle and upper part is connected with transparent panel 53, and the position of its opening and the first half before housing coaming plate 21 and inside casing coaming plate 22 be corresponding, and to be provided with opened front corresponding.Square year described cell container frame component 6 is made up of upper frame plate 61, lower frame plate 62, square pillar 63, a year cell container 67, upper frame plate 61 is connected with lower frame plate 62 by four square pillars 63, upper frame plate 61 is provided with container hole 64, corresponding container hole 64 is provided with container bottom outlet 65 on lower frame plate 62, lower frame plate 62 is also provided with multiple through hole 66, be placed with in the container hole 64 of upper frame plate 61 and carry cell container 67, carry cell container 67 lower end and be connected with container bottom outlet 65.

Claims (5)

1. a cell in vitro sterile culture device, it is by upper cover assembly, box assembly, hinge, square water box, square interior box assembly, square year cell container frame component composition, it is characterized in that: two upper cover assemblies (1) can be connected with box assembly (2) respectively by hinge (3) in folding, during connection, two opening surroundings of two opening cover plate (26) are tightly connected the back-shaped gasket (14) of two upper cover assembly (1) lower ends with the band on box assembly (2) respectively, the left side be separated into by two middle risers (25) in the inside casing coaming plate (22) of box assembly (2), respectively by upper toroidal membrane (29) in right two inner chambers, lower toroidal membrane (30), matrix pattern dividing plate (31) is connected with square interior box assembly (5), in square interior box assembly (5), be placed with square water box (4) and be connected with square year cell container frame component (6) by its support plate (52).
2. a kind of cell in vitro sterile culture device according to claim 1, it is characterized in that: described upper cover assembly (1) is by outer spill frid (11), concave shaped frid (12), back-shaped banded plate (13), back-shaped gasket (14), U-shaped handle (15), UV-lamp (16) is formed, the following peripheral of outer spill frid (11) is connected by the following peripheral of back-shaped banded plate (13) with concave shaped frid (12), adiabator layer is connected with outside between spill frid (11) and concave shaped frid (12), back-shaped banded plate (13) is connected with back-shaped gasket (14), spill frid (11) is fixed with U-shaped handle (15) above outside, UV-lamp (16) is had at concave shaped frid (12) internal fixtion.
3. a kind of cell in vitro sterile culture device according to claim 1, is characterized in that: described box assembly (2) is by housing coaming plate (21), inside casing coaming plate (22), left side vertical plate (23), right side riser (24), middle plate (25), the two opening cover plate (26) of band, base plate (27), bearing (28), upper toroidal membrane (29), lower toroidal membrane (30), matrix pattern dividing plate (31), bottom surface dividing plate (32), frame plate (33), glass (34), gas concentration lwevel controller (35), temperature regulator (36), device for control ultra violet lamp (37), rectangle ceramic heating plate (38), square ceramic hot-plate (39), dense carbon dioxide sensor (40), temperature sensor (41), carbon dioxide nozzle assembly (42) is formed, and is placed with inside casing coaming plate (22), is connected to left side vertical plate (23) between housing coaming plate (21) and inside casing coaming plate (22) in housing coaming plate (21), right side riser (24), is connected with two middle risers (25) at inside casing coaming plate (22) middle part, between two middle risers (25), is connected with adiabator layer, in inside casing coaming plate (22) periphery by housing coaming plate (21), left side vertical plate (23), right side riser (24) is connected with adiabator layer, base plate (27) is connected with below housing coaming plate (21) and inside casing coaming plate (22), bearing (28) is connected with, the left side be separated into by two middle risers (25) in inside casing coaming plate (22) below base plate (27), bottom surface dividing plate (32) is connected to below in right two inner chambers, adiabator layer is connected with in the space that bottom surface dividing plate (32) and base plate (27) are formed, on housing coaming plate (21) and inside casing coaming plate (22), be connected with the two opening cover plate (26) of band, two openings on the two opening cover plate (26) of band respectively with a left side, right two inner chambers are corresponding, before housing coaming plate (21) and inside casing coaming plate (22), first half correspondence is provided with opened front, is connected with frame plate (33), is connected with double glazing (34) in frame plate (33) in two opened front inner circumferential, on a left side, toroidal membrane (29) is all horizontally connected with in right two inner chambers, lower toroidal membrane (30), the left side of inside casing coaming plate (22) between upper toroidal membrane (29) and lower toroidal membrane (30) above and inside middle riser (25), right, be fixed with rectangle ceramic heating plate (38) below respectively, inside casing coaming plate (22) between lower toroidal membrane (30) and bottom surface dividing plate (32) and middle riser (25) inner side before, after, left, the right side is fixed with respectively rectangle ceramic heating plate (38), matrix pattern dividing plate (31) is connected with on bottom surface dividing plate (32), square ceramic hot-plate (39) is fixed with respectively in its matrix pattern dividing plate (31), also be sealedly connected with two carbon dioxide nozzle assemblies (42) by inside casing coaming plate (22) and adiabator layer below at housing coaming plate (21), carbon dioxide nozzle assembly (42) the inner puts in a left side respectively, in right two inner chambers, its outer end is connected with carbonic acid gas leaving air control valve, on a left side respectively by pipeline, both sides in right two inner chambers are fixed with dense carbon dioxide sensor (40) respectively, temperature sensor (41), is connected to gas concentration lwevel controller (35) from top to bottom in both sides above at housing coaming plate (21), temperature regulator (36), device for control ultra violet lamp (37), left at it, the gas concentration lwevel controller (35) on right both sides respectively with a left side, dense carbon dioxide sensor (40) and the corresponding carbonic acid gas leaving air control valve of right two inner chambers connect, left at it, the temperature regulator (36) on right both sides respectively with a left side, the temperature sensor (41) of right two inner chambers, rectangle ceramic heating plate (38), square ceramic hot-plate (39) connects, left at it, the device for control ultra violet lamp (37) on right both sides respectively with a left side, the UV-lamp (16) of right inside spill frid (12) internal fixtion connects.
4. a kind of cell in vitro sterile culture device according to claim 1, it is characterized in that: described square interior box assembly (5) is by interior box main body (51), support plate (52), transparent panel (53) is formed, interior box main body (51) is square structure, its upper end is the opening of inside flanging, two sides in interior box main body (51) and middle and upper part are below connected with support plate (52), the opening of interior box main body (51) above middle and upper part is connected with transparent panel (53), the position of its opening with housing coaming plate (21) and inside casing coaming plate (22) above the first half corresponding to be provided with opened front corresponding.
5. a kind of cell in vitro sterile culture device according to claim 1, it is characterized in that: described square year cell container frame component (6) be by upper frame plate (61), lower frame plate (62), Fang Zhizhu (63), carry cell container (67) to form, upper frame plate (61) is connected with lower frame plate (62) by four square pillars (63), upper frame plate (61) is provided with container hole (64), corresponding container hole (64) is provided with container bottom outlet (65) on lower frame plate (62), lower frame plate (62) is also provided with multiple through hole (66), be placed with in the container hole (64) of upper frame plate (61) and carry cell container (67), carry cell container (67) lower end to be connected with container bottom outlet (65).
CN201420661401.7U 2014-11-07 2014-11-07 A kind of cell in vitro sterile culture device Expired - Fee Related CN204174219U (en)

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Application Number Priority Date Filing Date Title
CN201420661401.7U CN204174219U (en) 2014-11-07 2014-11-07 A kind of cell in vitro sterile culture device

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Application Number Priority Date Filing Date Title
CN201420661401.7U CN204174219U (en) 2014-11-07 2014-11-07 A kind of cell in vitro sterile culture device

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI567190B (en) * 2015-10-02 2017-01-21 Liu Lun-Guang Cell incubator

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI567190B (en) * 2015-10-02 2017-01-21 Liu Lun-Guang Cell incubator

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