CN103205373B - Quality clostridium butyricum for breeding piglets - Google Patents
Quality clostridium butyricum for breeding piglets Download PDFInfo
- Publication number
- CN103205373B CN103205373B CN201310082656.8A CN201310082656A CN103205373B CN 103205373 B CN103205373 B CN 103205373B CN 201310082656 A CN201310082656 A CN 201310082656A CN 103205373 B CN103205373 B CN 103205373B
- Authority
- CN
- China
- Prior art keywords
- clostridium butyricum
- clostridium
- butylicum
- butyricum
- feed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fodder In General (AREA)
Abstract
Disclosed is quality clostridium butyricum for breeding piglets. The quality clostridium butyricum is capable of maintaining and regulating micro-ecological balance and improving immune functions of the piglets. The technical scheme includes that the clostridium butyricum HM-18 is preserved in the China General Microbiological Culture Collection Center on 29th October, 2012, and the preservation number of the clostridium butyricum is CGMCC No.6731. The invention further discloses an application method for the quality clostridium butyricum.
Description
Technical field
The present invention relates to a strain clostridium butylicum, specifically a strain maintains and the good clostridium butylicum for suckling piglet cultivation of microecosystem regulation balance, enhancing body immunologic function.
Background technology
Along with improving constantly of economic development and standard of living, people more and more recognize the basic meaning of " green ".But due to the driving of economic interests, antibiotic use has started to spread unchecked, particularly in feed, use in a large number, cause normal microflora imbalance in animal body and suppress the formation of organism immune response, the serious superinfection that causes, even can cause death; Secondly, residual in livestock and poultry body and in the food such as meat, egg, milk of microbiotic, can not make its deactivation completely through heat treated, and people's edible this based food for a long time, may cause chronic poisoning etc.If use probiotics in feed, will greatly reduce antibiotic consumption, solve the problem that microbiotic can't resolve, enhance productivity, advance to the direction of green agriculture.
Clostridium butyricum active bacteria formulation is in the market all from Japanese import, and price is higher, and clostridium butyricum has broad application prospects as medicine, protective foods, fodder additives and microbial fertilizer.If clostridium butyricum as fodder additives, can make our livestock products towards green future development, whole livestock industry and feed industry are had to profound significance.
The present invention is directed to above-mentioned problem, develop a kind of good clostridium butylicum HM-18(Clostridium butyricum HM-18 of suckling piglet cultivation), feed after suckling piglet as fodder additives, can be in suckling piglet enteron aisle antagonism animal pathogen, maintain and microecosystem regulation balance, enhancing body immunologic function, and produce amylase, VITAMIN, promote animal digesting and assimilating and trophism feed.
Summary of the invention
The object of the present invention is to provide a strain to maintain and the good clostridium butylicum for suckling piglet cultivation of microecosystem regulation balance, enhancing body immunologic function.
The present invention is achieved through the following technical solutions.
One strain is for the good clostridium butylicum of suckling piglet cultivation, it is characterized in that: described clostridium butylicum HM-18(Clostridium butyricum HM-18) on October 29th, 2012, it is great Tun road, Chaoyang District, city of BeiJing, China in address, the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, preserving number: CGMCC No.6731.
Clostridium butyricum HM-18 separates in health pig enteron aisle, Clostridium butyricum HM-18 can be in animal intestinal antagonism animal pathogen, maintain and microecosystem regulation balance, strengthen the immunologic function of animal, and produce amylase, VITAMIN, promote animal digesting and assimilating and trophism feed.
An application for clostridium butylicum, is characterized in that: for improving suckling piglet production performance and diarrhoea aspect.
A kind of activation of clostridium butylicum and cultivation, it is characterized in that: the Clostridium butyricum HM-18 of freezing preservation is inoculated in GPY liquid nutrient medium, at 37 DEG C of temperature, cultivate 20h, so go down to posterity and cultivate the activation Clostridium butyricum HM-18 bacterial classification described in obtaining for 2-3 time; Described GPY liquid nutrient medium is composed as follows: peptone 10.0g, yeast extract paste 10.0g, glucose 6.0g, MgSO
47H
2o1.0g, K
2hPO
42.0g, MnSO
450mg, adds 1L distilled water, regulates pH to 6.8,121 DEG C of sterilizing 15min.
The using method of clostridium butylicum in feed, is characterized in that: the bacterium powder after strain fermentation, freeze-drying, pulverizing and basal diet are stirred stand-by, in feed, clostridium butyricum active bacteria number reaches 1 × 10
5cfu/g.
Clostridium butyricum HM-18 provided by the invention has following positively effect:
Experimental result shows, in weanling pig feed, adds 1 × 10
5the clostridium butylicum of cfu/g, can significantly improve the speed of growth of weanling pig, has saved feed intake, thereby can inference, adds clostridium butyricum, can improve the economic benefit of weanling pig production.
Test-results shows, in weanling pig feed, adds 1 × 10
5the clostridium butylicum of cfu/g, can reduce the incidence of diarrhea of weaned piglets, and then can reduce the generation of intestines source property disease, improves feed digestibility, improves pig starter feed utilization ratio.
Brief description of the drawings
Accompanying drawing is that diarrhea of weaned piglets improves situation schematic diagram.
Embodiment
Below in conjunction with embodiment, the invention will be further described; Following embodiment is illustrative, does not limit the scope of the invention.
Experimental technique in following embodiment, if no special instructions, is ordinary method.
One strain maintains the good clostridium butylicum for suckling piglet cultivation with microecosystem regulation balance, enhancing body immunologic function, it is characterized in that: described clostridium butylicum HM-18Clostridium butyricum HM-18 is on October 29th, 2012, it is great Tun road, Chaoyang District, city of BeiJing, China in address, the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, preserving number: CGMCC No.6731.
Clostridium butyricum HM-18 separates in health pig enteron aisle, Clostridium butyricum HM-18 can be in animal intestinal antagonism animal pathogen, maintain and microecosystem regulation balance, strengthen the immunologic function of animal, and produce amylase, VITAMIN, promote animal digesting and assimilating and trophism feed.
An application for clostridium butylicum, is characterized in that: for improving suckling piglet production performance and diarrhoea aspect.
A kind of activation of clostridium butylicum and cultivation, it is characterized in that: the Clostridium butyricum HM-18 of freezing preservation is inoculated in GPY liquid nutrient medium, at 37 DEG C of temperature, cultivate 20h, so go down to posterity and cultivate the activation Clostridium butyricum HM-18 bacterial classification described in obtaining for 2-3 time; Described GPY liquid nutrient medium is composed as follows: peptone 10.0g, yeast extract paste 10.0g, glucose 6.0g, MgSO
47H
2o1.0g, K
2hPO
42.0g, MnSO
450mg, adds 1L distilled water, regulates pH to 6.8,121 DEG C of sterilizing 15min.
The using method of clostridium butylicum in feed, is characterized in that: the bacterium powder after strain fermentation, freeze-drying, pulverizing and basal diet are stirred stand-by, in feed, clostridium butyricum active bacteria number reaches 1 × 10
5cfu/g.
The application of described clostridium butylicum aspect Production Performance of Weaning Pigs, concrete grammar is as follows:
Testing program: select kind be long white and Large White hybridize 1 generations 28 20 of the sodium selenites that wean of age in days, be divided at random 2 groups, every group of 10 piglets, 35 days test periods; Control group fed typical case corn bean pulp type basis day, test group is fed and is added the basal diet of clostridium butyricum.Basal diet and trophic level are in table 1.
Table 1 basal diet formula and trophic level
Index determining: average daily ingestion amount: accurately weigh and record test group and control group feeding coal every day, weigh when off-test and record clout amount, calculate the average daily ingestion amount of every piglet; Average daily gain: the first day (beginning) of test, 8 title piglets in the 35th day (end) morning weight on an empty stomach, the average daily gain of calculating every piglet; Material anharmonic ratio: calculate test group and control group material anharmonic ratio according to average daily ingestion amount and average daily gain; Diarrhea rate: after on-test, observe piglet ight soil situation every day, records test group and control group diarrhoea piglet head, and a number of times that finally with every group of piglet, diarrhoea occurs, divided by total raising number of times, calculates diarrhea rate.
Experimental animal management: test piglet adopts high bed to raise, semi-open pig house, natural ventilation, piglet free choice feeding, nipple water dispenser is freely drunk water; Immunity and expelling parasite program are synchronously carried out by pig farm routine.Receiving tank external loss material is put back in groove in time, observes at any time, records and search for food and healthy state.
Experiment 1: the effect that clostridium butylicum HM-18(Clostridium butyricum HM-18) improves Production Performance of Weaning Pigs.
Select kind be long white and Large White hybridize 1 generations 28 20 of the sodium selenites that wean of age in days, be divided at random 2 groups, every group of 10 piglets, control group fed basal diet, test group is fed and is added the basal diet of clostridium butylicum, clostridium butyricum viable count>=1 × 10 in feed
5cfu/g, 35 days test periods.Wean suckling piglet production performance improvement situation is in table 2.
Table 2 Production Performance of Weaning Pigs improves situation
As seen from the experiment, add butyric acid shuttle shape gemma bar in daily ration, the weanling pig speed of growth significantly improves compared with control group, and average daily gain amount is 70 grams.
Experiment 2: clostridium butylicum HM-18(Clostridium butyricum HM-18) improve the effect of diarrhea of weaned piglets.
Select kind be long white and Large White hybridize 1 generations 28 20 of the sodium selenites that wean of age in days, be divided at random 2 groups, every group of 10 piglets.30 days test periods, test adopts basal diet composition and trophic level in table 1.After on-test, observe piglet ight soil situation every day, records test group and control group diarrhoea piglet head, and a number of times that finally with every group of piglet, diarrhoea occurs, divided by total raising number of times, calculates diarrhea rate.Test grice diarrhoea rate is shown in accompanying drawing.
As seen from the experiment, add clostridium butylicum in daily ration, diarrhea of weaned piglets rate is significantly increased compared with control group, and diarrhea rate is lower by 34.7%.
Claims (4)
- One strain for the clostridium butylicum of suckling piglet cultivation ( clostridium butyricum) HM-18, its preserving number is CGMCC No.6731.
- Clostridium butylicum as claimed in claim 1 ( clostridium butyricum) application of HM-18 in the fodder additives for the preparation of improving aspect suckling piglet production performance and diarrhoea.
- One kind as claimed in claim 1 clostridium butylicum ( clostridium butyricum) activation of HM-18 and the method for cultivation, it is characterized in that: by freezing preservation clostridium butyricumhM-18 is inoculated in GPY liquid nutrient medium, at 37 DEG C of temperature, cultivates 20h, so goes down to posterity and cultivates the activation described in obtaining for 2-3 time clostridium butyricumhM-18 bacterial classification; Described GPY liquid nutrient medium is composed as follows: peptone 10.0g, yeast extract paste 10.0g, glucose 6.0g, MgSO 47H 2o1.0g, K 2hPO 42.0g, MnSO 450mg, adds 1L distilled water, regulates pH to 6.8,121 DEG C of sterilizing 15min.
- One kind as claimed in claim 1 clostridium butylicum ( clostridium butyricum) using method of HM-18 in feed, it is characterized in that: the bacterium powder after strain fermentation, freeze-drying, pulverizing and basal diet are stirred stand-by, in feed, clostridium butyricum active bacteria number reaches 1 × 10 5cfu/g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310082656.8A CN103205373B (en) | 2013-03-15 | 2013-03-15 | Quality clostridium butyricum for breeding piglets |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310082656.8A CN103205373B (en) | 2013-03-15 | 2013-03-15 | Quality clostridium butyricum for breeding piglets |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103205373A CN103205373A (en) | 2013-07-17 |
| CN103205373B true CN103205373B (en) | 2014-09-03 |
Family
ID=48752782
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310082656.8A Active CN103205373B (en) | 2013-03-15 | 2013-03-15 | Quality clostridium butyricum for breeding piglets |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103205373B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106479924B (en) * | 2016-10-31 | 2018-06-12 | 湖北绿雪生物科技有限公司 | The preparation and application of a kind of clostridium butyricum and clostridium butyricum active bacteria preparation |
| CN106819487A (en) * | 2017-03-10 | 2017-06-13 | 天津市畜牧兽医研究所 | A kind of low albumen mixed feed of piglet containing clostridium butyricum and application |
| CN106922987B (en) * | 2017-03-16 | 2018-08-07 | 安徽省新康饲料有限公司 | A kind of complete feed preparation method based on clostridium butyricum |
| CN107970446B (en) * | 2017-10-31 | 2020-11-03 | 广西壮族自治区兽医研究所 | Piglet diarrhea-resistant high-immunity whole egg powder microcapsule and preparation method and application thereof |
| CN115211492B (en) * | 2022-06-07 | 2024-04-19 | 圣道生物技术(山东)有限公司 | Stress-resistant growth-promoting stress-relieving medicine for livestock and poultry, and preparation method and application thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102226162B (en) * | 2011-05-23 | 2012-11-28 | 江苏省微生物研究所有限责任公司 | Preparation method and application of composite microbial feed additive |
-
2013
- 2013-03-15 CN CN201310082656.8A patent/CN103205373B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN103205373A (en) | 2013-07-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102168045B (en) | Bacillus subtilis preparation and preparation method thereof | |
| CN103039699B (en) | Mixed fermentation feed and preparation method and application method of mixed fermentation feed | |
| CN102204632B (en) | Microbial feed additive for increasing livestock and poultry product quality and improving meat egg milk flavor and preparation method thereof | |
| CN101463329B (en) | Saccharomyces cerevisiae, yeast preparation including the same, feed, and preparation and use thereof | |
| CN102226162B (en) | Preparation method and application of composite microbial feed additive | |
| CN107173522A (en) | A kind of fermented feed containing probiotics and its preparation method and application | |
| CN104472879A (en) | Tea residue feed additive and preparation method thereof | |
| CN105475624A (en) | Microbial fermentation feed as well as production method and application thereof | |
| CN103966124A (en) | Compound microecological preparation and additive, as well as premix thereof | |
| CN103981118A (en) | Bacillus subtilis feed additive and preparation method and application thereof | |
| CN104397323A (en) | Microbial feed additive | |
| CN101974463B (en) | Lactobacillus reuteri and composite viable bacteria preparation thereof | |
| CN103205373B (en) | Quality clostridium butyricum for breeding piglets | |
| CN103205375B (en) | Quality clostridium butyricum for breeding egg-laying poultry | |
| CN103907749A (en) | Biological fermentation active carrier type composite premix feed as well as preparation method and application thereof | |
| CN102907563A (en) | Method for preparing high-activity probiotic preparation for livestock breeding | |
| CN101971920A (en) | Porcine lactobacillus reuteri lyophilized preparation and preparation method thereof | |
| CN105360615A (en) | Fermented feed raw material preparation as well as preparation method and application thereof | |
| CN111647541B (en) | Clostridium butyricum viable bacteria preparation, production method thereof and animal feed additive | |
| CN103609881A (en) | Meat sheep fermented concentrated feed containing probiotics and preparation method of feed | |
| CN103181460A (en) | Preparation method and application of lactobacillus plantarum for feed | |
| CN105146054A (en) | Straw bio-fermentation and feed preparation technique | |
| CN103255080B (en) | Excellent bacillus coagulans for breeding piglets and application thereof | |
| CN103255082B (en) | One plant of excellent bacillus coagulans and its application for growing and fattening pigs cultivation | |
| CN109430559A (en) | A kind of preparation method of the liquid active feed for piglet |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |