CN103254320B - The method of Production by Enzymes W-Gum - Google Patents
The method of Production by Enzymes W-Gum Download PDFInfo
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Abstract
The invention provides a kind of method of Production by Enzymes W-Gum, comprise the steps: that corn just soaks; Corn soaks again; Fragmentation and separated plumule; Fine grinding, separated fiber; Centrifugation; Washing and dehydration; Drying, the technique that wherein corn soaks again is adopt the process water in technique water pot to carry out immersion treatment again in the first soaking corn of described discharge soak solution, then the immersion treatment time is 5 ~ 7 hours, carries out screening process after immersion treatment; Obtain the soaking corn again through immersion treatment again and the 3rd soak solution.Present invention employs twice soaking technology, and with the addition of zymin soaking in operation again, first immersion treatment can change structure and the physical properties of corn embryosperm; And with the addition of the characteristic that zymin can utilize zymin soaking again in operation, promote that the cell walls of corn kernel and seed coat connect fiber ruptures or separation, be more conducive to zymin through the corn of first immersion treatment simultaneously and play a role.
Description
Technical field
The invention belongs to corn deep processing field, be specifically related to a kind of method of Production by Enzymes W-Gum.
Background technology
W-Gum is raw material with corn grain, soaked, the product that crushing and screening, separating, washing, dewatered drying are made by sulfurous acid.W-Gum is except being directly used in food, papermaking, weaving, medicine and other fields, most for deep processing.Starch industry is the industry that China's corn usage quantity is larger, the output of China's W-Gum from 1999 to 2011 in rising tendency year by year.Within 2009, national W-Gum amount reaches 2,170 ten thousand tons, occupies second place of the world.β-amylose take W-Gum as the high-grade products of raw material, has higher economic worth and edibleness, be widely used in the industries such as food, medicine, chemical industry, fermentation.The β-amylose that W-Gum is produced comprises glucose, fructose, maltose etc.The maize treacle that W-Gum deep processing is made easily is absorbed by the body utilization, is the excellent sweeting agent making candy, cake, beverage and can.Sorbyl alcohol is the derivative of β-amylose, and mainly for the production of vitamins C, recent year is in great demand.The modified starch that the basis of starch is carried out modification is the product that new development is in recent years got up, of many uses, developed more than 3000 kind abroad, and China only develops more than 50 kind.It is widely used in the industry such as papermaking, weaving, food, feed, medicine, daily use chemicals, oil, and what usage quantity was maximum is the industries such as papermaking, food and textiles, has good prospects.Therefore, the demand of recent domestic to W-Gum increases.
In addition, in production of corn starch process, except W-Gum major product, also can obtain Semen Maydis oil, zein fiber, protein powder and corn steep liquor three kinds of byproducts.The unsaturated fatty acid content of Semen Maydis oil reaches more than 85%, and mainly contain oleic acid and linolic acid, human absorptivity reaches more than 97%; Containing sitosterol in Semen Maydis oil, there is the effect suppressing cholesterol to increase; Rich in vitamin E, for human body cell division, delaying senility has certain effect.Corn dietary fiber is made up of Mierocrystalline cellulose and hemicellulose, has effect that is hypotensive, prevention cardiovascular and cerebrovascular diseases.Protein powder is the significant by-products of corn wet processing, and its protein content, up to 50% ~ 70%, mainly as the raw material producing high protein feed, also can be used to produce other Industrial products such as prolamine.Protein content total in corn steep liquor is 44% ~ 48% (butt), and as feed formulation, corn steep liquor is a kind of high protein, high-octane nutrition, simultaneously containing abundant vitamins B and mineral substance.
At present, W-Gum processing generally adopts wet method processing technology in the world.In corn starch, protein, plumule, fiber etc. mainly adopt the mechanical means such as pulverizing, screening, centrifugal, extruding, filtration to carry out being separated, concentrated and dry.And those skilled in the art also adopts multiple method to improve the complete processing of W-Gum, such as patent publication No. is that the Chinese invention patent of 101372702A discloses a kind of method shortening the corn soaking time in production of corn starch process, and the method utilizes high pressure and prozyme to combine immersion thus shortens the time of corn soaking.But the method adopts high pressure hydration to be unfavorable for especially applying in the industrial production, this method complex process, and its equipment again input cost is high, and tooling cost is high, and energy consumption is large, and shorten the corn soaking time with this, in reality, operability is not strong.In addition, patent publication No. is that the Chinese invention patent of CN1831013A also discloses a kind of corn starch soaking prodn. process of adding vegetable protease, in corn steep liquor, the lactobacillus inoculation through fermentation culture is added in the immersion process of the method the first step, in the corn steep liquor of the 3rd step fragmentation, add plant protease with abundant degrade proteins matrix release starch, and then shorten soak time further.Disclosed in above-mentioned two patents, method is all add proteolytic enzyme in soak solution, but proteolytic enzyme can by the partial proteolysis in W-Gum, this finally can cause production of corn starch process to have the loss of the protein of economic worth most, and be dissolved in soluble proteins in water also can the process water of disposal of pollutants, be unfavorable for environmental protection.Therefore, separating effect and the purity of the isolate in the production of corn starch process of prior art are limited, and deep processing cost for purification is high, and the energy consumption of isolate sepn process (water, electricity, steam) is high, efficiency is low.
Summary of the invention
Based on this, be necessary for prior art Problems existing, a kind of method of Production by Enzymes W-Gum is provided.The method is on the basis of traditional wet method processing technology, have employed twice soaking technology, and adds zymin in soaking technology again, to improve mechanical separation effect and separation efficiency, and then effectively improves isolate purity, recovery rate.In addition, adopt method of the present invention not only can improve the yield of W-Gum, also can improve the yield of protein powder, and simultaneously can effective energy efficient.
Technical scheme of the present invention is as follows:
A method for Production by Enzymes W-Gum, comprises the steps:
(1) corn just soaks: by corn soaking in the first soak solution, discharges soak solution, obtain the first soaking corn through first immersion treatment and the second soak solution, become corn steep liquor after described second soak solution evaporation concentration when moisture in corn is greater than 40%;
(2) corn soaks again: adopt the process water in technique water pot to carry out immersion treatment again in the first soaking corn of described discharge soak solution, then the immersion treatment time is 5 ~ 7 hours, carries out screening process after immersion treatment; Obtain the soaking corn again through immersion treatment again and the 3rd soak solution;
(3) broken and separated plumule: obtain the first mixture and separated plumule after the soaking corn again that step (2) obtains is carried out broken apart plumule process;
(4) fine grinding, separated fiber: the first mixture that step (3) obtains is carried out fine grinding process and obtains the second mixture, next separating treatment is carried out to the second mixture and obtain the 3rd mixture and fiber yarn;
(5) centrifugation: the 3rd mixture obtained step (4) carries out centrifuging treatment and obtains Corn Starch Blends and protein mixture;
(6) washing and dehydration: the Corn Starch Blends obtained step (5) carries out washing and processed obtains wet corn starch;
(7) dry: the W-Gum that drying treatment obtains drying is carried out to the wet corn starch that step (6) obtains.
Wherein in an embodiment, in step (1), the concentration of described first soak solution sulfite is 0.15% ~ 0.25%, and soaking temperature is 45 DEG C ~ 50 DEG C, and soak time is 40 ~ 60 hours.
Wherein in an embodiment, in step (2), containing zymin in the process water in described technique water pot, the consumption of described zymin is 0.005% ~ 0.01% of corn gross weight, then the temperature of soaking is 30 DEG C ~ 55 DEG C, and pH is 3.0 ~ 6.0.
Wherein in an embodiment, the 3rd soak solution obtained in step (2) is back to technique water pot and recycles.
Wherein in an embodiment, in step (4), also comprise filametntary carrying out washing treatment, the washings that carrying out washing treatment obtains is mixed to the 3rd mixture.
Wherein in an embodiment, described zymin is the combination of cellulase and zytase, and the content of wherein said cellulase is 20% ~ 40% by weight, and the content of described zytase is 60% ~ 80% by weight.
Wherein in an embodiment, the content of described cellulase is 30% ± 1% by weight, and the content of described zytase is 70% ± 1% by weight.
Wherein in an embodiment, described cellulase is obtained by Li Shi Trichoderma kind, and in PH3.8 ~ 4.2, under the condition of temperature 50 C, the transformation period of described cellulase is 96 ~ 144 hours.
Wherein in an embodiment, described zytase is obtained by aspergillus niger strain, and in PH3.8 ~ 4.2, under the condition of temperature 50 C, the transformation period of described zytase is 96 ~ 144 hours.
Wherein in an embodiment, what described carrying out washing treatment adopted is all with the addition of the process water in the technique water pot of zymin.
The invention has the beneficial effects as follows:
1, present invention employs twice soaking technology, and with the addition of zymin soaking in operation, first immersion treatment can change structure and the physical properties of corn embryosperm again, weaken the connecting key in protein, reduce the physical strength of corn kernel, lixiviate goes out part soluble substance; And with the addition of the characteristic that zymin can utilize zymin soaking again in operation, promote that the cell walls of corn kernel and seed coat connect fiber ruptures or separation, be more conducive to zymin through the corn of first immersion treatment simultaneously and play a role.
2, method of the present invention can utilize existing wet method starch processing device, do not increase new energy consumption, improve the functioning efficiency of zymin, reduce the interpolation cost of zymin, and zymin can recycle, improve mechanical separation effect and separation efficiency; Reduce bath water amount, improve detersive efficiency; Improve centrifugal efficiency, and then reduce power consumption, improve economic benefit, there is great using value.
3, adopt method of the present invention not only can improve the yield of W-Gum, also can improve the yield of protein powder.Because the present invention does not add proteolytic enzyme, can proteolytic be avoided, decrease the loss that production of corn starch process has the protein of economic worth most, decrease the pollution of soluble proteins in discharge water.
4, the present invention adopt Li Shi Trichoderma kind to obtain cellulase in the content of coenzyme proteolytic enzyme low, therefore enzymolysis is avoided to increase solvend, so decrease sewage discharge, be more conducive to environmental protection, and the minimizing of the solvend of enzymolysis is also conducive to improving separating effect and product purity.
5, zymin long half time of the present invention, makes that the process water containing zymin is sustainable in recycling to play a role, and reduces the input cost of zymin.
6, the enzyme described in the present invention is cellulase or zytase or the combination of the two.The characteristic that the present invention utilizes cellulase the cell walls in corn kernel and seed coat can be connected fiber ruptures or be separated with hemicellulase, makes plant cell wall decompose very soon or collapses to make effective decomposition of the Mierocrystalline cellulose in corn kernel, hemicellulose can promote that the materials such as seed coat, endosperm starch, protein, plumule, fiber are effectively separated.The present invention also utilizes zytase can reduce the wetting ability of Mierocrystalline cellulose and hemicellulose to greatest extent simultaneously, therefore, it is possible to reduce fibrous matter water content, so improve concentrated and drying efficiency, reduce concentrated and drying quantity of steam, and then greatly save energy consumption.
Accompanying drawing explanation
Below in conjunction with concrete drawings and the specific embodiments, the present invention is further elaborated.
Fig. 1 is the schematic flow sheet of the method for Production by Enzymes W-Gum of the present invention.
Embodiment
Zymin described is in the present invention the combination of cellulase or zytase or cellulase and zytase.Cellulase of the present invention and zytase Dou Shi Baiyin Sino Biotechnology Co., Ltd. carry out the product of fermentative production according to the bacterial classification survival temperature that Chinese industrial Microbiological Culture Collection administrative center records.
The concrete preparation method of described cellulase is as follows:
Raw material: Chinese industrial Microbiological Culture Collection administrative center provides Li Shi Trichoderma kind (numbering 13051)
Preparation method:
1. slant strains preparation: mould for wood (13051) strain bacterial classification is aseptically transferred to PDA medium slant, cultivates 1 ~ 2 day at 30 DEG C, and treating to cover with taking-up in inclined-plane, to be put in 4 DEG C of refrigerations for subsequent use.
2. the preparation on eggplant bottle inclined-plane: slant strains is aseptically transferred to PDA eggplant bottle inclined-plane, cultivates 1 ~ 2 day at 30 DEG C, and treating to cover with taking-up in eggplant bottle inclined-plane, to be put in 4 DEG C of refrigerations for subsequent use.
3. the preparation of spore suspension: by eggplant bottle inclined-plane with the ratio of inoculum size 6%, aseptically proceeds in the sterilized water of 5 liters of triangular flasks, is aseptically proceeded to by the spore suspension in triangular flask in 5 liters of inoculation bottles.
4. seed enlarged culturing: stalk, wheat bran and inorganic salt etc. are dropped in the seeding tank of 6m3 according to a certain percentage, with tap water constant volume to 3m3, stir, after steam sterilizing, when temperature drops to 30 ~ 32 DEG C, access spore suspension, at air quantity than being 1:0.2 ~ 0.5, temperature is 30 ~ 32 DEG C, tank pressure is cultivate 28 ~ 36h under 0.05 ~ 0.07Mpa condition, obtains seed enlarged culturing kind liquid.
5. liquid submerged fermentation: stalk, wheat bran and inorganic salt etc. are dropped in the fermentor tank of 80m3 according to a certain percentage, with tap water constant volume to 50m3, stir, after steam sterilizing, seed enlarged culturing kind liquid when temperature drops to 30 ~ 32 DEG C, at air quantity than being 1:0.2 ~ 0.5, temperature is 30 ~ 32 DEG C, tank pressure is cultivate 98 ~ 110h under 0.05 ~ 0.08Mpa condition, obtains fermentation liquid.
6. the purification of cellulase: utilize polypropylene flame filter press that fermentation liquid is carried out solid-liquid separation, obtain filter residue and cellulase clear liquid, cellulase clear liquid adopts hollow-fibre membrane (molecular weight 10000 dalton) to carry out ultrafiltration and concentration about 7 times, squeezes into material-storage jar to be dried.
7. dry: the cellulase solution concentrated is adopted pressure type drying tower spraying dry, and inlet temperature 125 ~ 135 DEG C, temperature out 60 ~ 70 DEG C, obtains Solka-floc enzyme.
8. stdn: Solka-floc enzyme is added the carriers such as starch and is re-dubbed required product.
9. finished product: by composite good weight per unit length packaging, warehouse-in.
The concrete preparation method of described zytase is as follows:
Raw material: Chinese industrial Microbiological Culture Collection administrative center provides aspergillus niger strain (numbering 40613)
Preparation method:
1. slant strains preparation: aspergillus niger (40613) strain bacterial classification is aseptically transferred to PDA medium slant, cultivates 1 ~ 2 day at 30 DEG C, and treating to cover with taking-up in inclined-plane, to be put in 4 DEG C of refrigerations for subsequent use.
2. the preparation of seed suspension liquid: (lactose, wheat bran and inorganic salt etc. add according to a certain percentage slant strains aseptically to be proceeded to the 5 liters of triangle culturing bottles having prepared liquid nutrient medium, PH5.0 ~ 5.8, sterilizing) in, at shaking speed 180rpm ~ 200rpm, cultivate 36h under 29 ~ 32 DEG C of conditions, obtain zytase seed suspension liquid for subsequent use.
3. seed enlarged culturing: lactose, wheat bran and inorganic salt etc. are dropped in the seeding tank of 6m3 according to a certain percentage, with tap water constant volume to 3m3, stir, after steam sterilizing, when temperature drops to 29 ~ 32 DEG C, access zytase seed suspension liquid, at air quantity than being 1:1 ~ 1.2, temperature is 29 ~ 32 DEG C, tank pressure is 0.05 ~ 0.07Mpa, cultivates 32 ~ 36h, obtain seed enlarged culturing kind liquid under rotating speed 180rpm ~ 300rpm condition.
4. liquid submerged fermentation: lactose, wheat bran and inorganic salt etc. are dropped in the fermentor tank of 80m3 according to a certain percentage, with tap water constant volume to 50m3, stir, after steam sterilizing, when temperature drops to 30 ~ 32 DEG C, proceed to seed enlarged culturing kind liquid, at air quantity than being 1:1 ~ 1.2, temperature is 29 ~ 32 DEG C, tank pressure is 0.05 ~ 0.08Mpa, cultivates 140 ~ 150h, obtain fermentation liquid under rotating speed 180rpm ~ 300rpm condition.
5. the purification of zytase: utilize polypropylene flame filter press that fermentation liquid is carried out solid-liquid separation, obtain filter residue and zytase clear liquid, zytase clear liquid adopts hollow-fibre membrane (molecular weight 10000 dalton) to carry out ultrafiltration and concentration about 7 times, squeezes into material-storage jar to be dried.
6. dry: the zytase liquid concentrated is adopted pressure type drying tower spraying dry, and inlet temperature 125 ~ 135 DEG C, temperature out 60 ~ 70 DEG C, obtains powdery zytase.
7. stdn: powdery zytase is added the carriers such as starch and is re-dubbed required product.
8. finished product: by composite good weight per unit length packaging, warehouse-in.
The production stage that zymin of the present invention can be added into W-Gum with process water refers to: the zymin of solid is dissolved with 5 ~ 10 times of process waters, add volume according to commodity corn processing flow rate calculation enzyme liquid stream, the addition of zymin is that 0.005-0.01%(is with commodity corn weighing scale).Such as: commodity corn 10 tons/hour, addition 0.01% that is 1000 grams, 10 times of process waters dissolve namely 10 liters.The stream of enzyme liquid adds volume that is 10 ls/h.
The present invention also can adopt liquid enzyme formulation directly to add in the production process of W-Gum.
As shown in Figure 1, specific operation process of the present invention is as follows:
1, raw material handling: what the present invention adopted is generally commodity corn, therefore needs before being processed to carry out raw material handling to corn, owing to containing various dust and weed-trifles, organic and inorganic impurity in commodity corn.In order to ensure safety in production and quality product, must clear up the impurity existed in corn.The method of cleaning corn mainly adopts screening, selection by winnowing etc.Cleaning equipment has vibratory screening apparatus, specific-gravity stoner, permanent magnetic pulley and wheat washer etc.
2, immersion treatment: the immersion of corn directly has influence on the normal production of later process starch, and what affect Starch Production goes out rate, Quality and yield.Therefore, corn soaking is one of very important operation in production of corn starch operation.The object of corn soaking is the structure and the physicochemical property that change endosperm, weakens the connecting key in protein, and reduce the physical strength of corn kernel, lixiviate goes out part soluble substance, and suppresses harmful activity of the microorganism come in Corn Belt.
Immersion of the present invention is divided into two steps, first carry out just soaking to corn, and then adopt the process water having added zymin to soak again corn, concrete technological process is: (1) corn just soaks: by corn soaking in the first soak solution, soak solution is discharged when moisture in corn is greater than 40%, obtain the corn through first immersion treatment and the second soak solution, after described second soak solution evaporation concentration, become corn steep liquor; (2) corn soaks again: adopt the process water in technique water pot to carry out immersion treatment again in the corn of described discharge soak solution, then the immersion treatment time is 5 ~ 7 hours, carries out screening process after immersion treatment; Obtain the corn through immersion treatment again and the 3rd soak solution.First the present invention adopts and just soaks the preliminary structure and the physicochemical property that change corn embryosperm, reduce the physical strength of corn kernel, lixiviate simultaneously goes out part soluble substance, and then adopt the process water adding zymin again to soak, because the structure of the corn through just soaking changes, therefore immersion at the beginning of is that the zymin in soaking again provides more favourable environment, facilitates zymin to play a role.
Corn soaking method is divided into static soak method and adverse current diffusion infusion method.Static soak is owned by France in single tank corn soaking, and namely the immersion water of each tank is not carried mutually.Adverse current diffusion infusion method is also diffusion process or multiple tank series connection counter-current.At present, most enterprise uses adverse current diffusion infusion method to carry out soaking corn.Adverse current diffusion process is several steeping tanks, pump and placed in series, according to the principle that adverse current is soaked, sulfurous acid soaks water and squeezes into together with fresh corn in tank unlike static method, but squeeze in the longest corn tank of soak time, immersion water is squeezed into the corn soaking tank of slightly short period of time immersion by the later pump that circulates, and like this immersion water is squeezed into another tank from a tank successively against the direction newly entering corn.The direction that the direction of sulfurous acid tank switching flowing and corn feed intake is contrary, and the direction that namely in corn, solubility dry matter content reduces is contrary with soaking the direction that in water, solubility dry substance concentration improves.What just soak employing in the present embodiment is adverse current diffusion infusion method, and soaks the static soak method that adopts again, and adverse current is spread infusion method by the present embodiment and static soak method combines, and zymin is better played a role, and improves the yield of final W-Gum.And the soak solution soaked again can be back to technique water pot to be continued to use, and saves the consumption of zymin.And the soak solution that the first immersion of two step infusion methods of the present invention obtains containing zymin, is not conducive to following process.
(3) broken and separated plumule: obtain the first mixture and separated plumule after the corn through immersion treatment again step (2) obtained carries out broken apart plumule process.
Corn is after soaking, and its physics, chemical property there occurs change, and the connecting key between plumule and endosperm weakens.Connecting key in corn embryosperm between protein and starch also weakens.Maize germ moisture content about 60% after soaking, has higher elasticity, and easily separates from corn grain when fragmentation.In addition, when fragmentation, endosperm starch matter part is also ground into particle, and therefrom discharges the starch within 25%.The object of corn fragmentation is that plumule separates with endosperm, and discharges the starch of some amount.
Soaked corn, discharges soak solution, and with the warm water corn steep liquors of 45 ~ 50 DEG C, corn is first sent into sandstone trapping swirler and remove sandstone, then send into gravity curved screen, separate conveying Water circulation, corn enters in corn hopper in order to entering crusher in crushing.The broken general method adopting twice fragmentation of corn, that is: corn → primary fragmentation → germ separation → secondary breaking → germ separation.Corn grain is through primary fragmentation, and most of plumule separates with endosperm, pulls plumule out, then enter secondary breaking by floating tank, and broken through twice, plumule is all separated.The plumule separated, be with the addition of the process water spray of zymin on the vibrating by continuous print, wash away the starch milk of plumule surface adhesion, seitan etc., in whizzer preliminary hydro-extraction to plumule, water content is less than 36%.
(4) fine grinding, separated fiber: the first mixture that step (3) obtains is carried out fine grinding process process and obtain the second mixture, next separating treatment is carried out to the second mixture and obtain the 3rd mixture and fiber yarn.
Corn after broken and separated plumule, containing endosperm particulate, seitan, cortex and partial starch particle.Major part starch is included in endosperm particle and cortex, must carry out meticulous grinding, could discharge starch, protein and Mierocrystalline cellulose to greatest extent, for good condition is created in the separation of later each component.The object of refining destroys the combination of starch and non-starch composition in corn fragment, starch dissociated out to greatest extent, separates fibrous residue, and protein in endosperm is separated with starch granules, to be separated further and to refine.Fiber separation mainly will discharge the fibrous residue after starch through repeatedly with the addition of the process water washing of zymin, make it contain less free starch and in conjunction with starch.Fiber after washing becomes dry slag crust through water squeezing, oven dry.Zymin of the present invention can reduce the wetting ability of Mierocrystalline cellulose and hemicellulose to greatest extent, therefore, it is possible to reduce fibrous matter water content, so improve concentrated and drying efficiency, reduces concentrated and drying quantity of steam, and then has greatly saved energy consumption.
(5) centrifugation: the 3rd mixture obtained step (4) carries out centrifuging treatment and obtains Corn Starch Blends and protein mixture.
Fecula Ruzhong after separated fiber also to grade non-starch class material containing more protein, fat, ash, and particularly protein content is high especially, must be separated, and just can obtain purer starch.
Starch and being separated in butterfly centrifugal machine of protein are carried out, due to the particle diameter of starch granules and proportion larger than albumen plasmid, its settling velocity in suspension is faster than protein particulate, and available separating centrifuge makes starch and protein separation efficiently.
(6) washing and dehydration: the Corn Starch Blends obtained step (5) carries out washing and processed obtains wet corn starch.
For removing solubility and insoluble protein, reduce starch acidity and improve suspension concentration, with new process water by ten grades of swirler countercurrent washings.After washing, starch horizontal type scraper centrifuger carries out mechanical dehydration, requires that the water content taken off to wet starch is 38% ~ 40%.
(7) dry: the W-Gum that drying treatment obtains drying is carried out to the wet corn starch that step (6) obtains.
Drying is the operation sequence utilizing heat energy to remove moisture in destarching.Air stream drying mode is adopted when starch is dry.Starch drying process condition controls: (1) wind speed, generally at 14 ~ 24m/s, often selects 17 ~ 20m, and wind speed is too low, and bulk wet feed can not be taken away with the wind, easily makes product heat damage.Wind speed is too high, and systemic resistance increase is too large, and product moisture is also wayward.(2), when air quantity gas-solid weight ratio is 5 ~ 10, moisture eliminator can normally run preferably.(3) time of drying is generally at 1 ~ 2s.(4) air themperature 140 ~ 160 DEG C.(5) blast is used for the various pressure-losseses of supplemental air flow drying tube.
The general moisture content 12 ~ 14% of dry starch.In order to ensure that finish fineness is even, sometimes also finished product arrangement will be carried out.First through screening process, sift out the starch of regulation fineness, screen overflow is sent into pulverizer and is pulverized, and then sieves, and makes product all reach the fineness of regulation.
Preferably, as a kind of embodiment, the concentration of described first soak solution sulfite is 0.15% ~ 0.25%, and soaking temperature is 45 DEG C ~ 50 DEG C, and soak time is 40 ~ 60 hours.
Because the environment of various places is different, so the corn soaking condition of various places factory is incomplete same.General operational requirement(GOR) is as follows: the sulfur dioxide concentration soaking water is 0.15% ~ 0.2%, and pH value is 3.5.In immersion process, sulfurous gas is by Maize Absorption, and concentration reduces gradually, and in final immersion water of discharging, the concentration of sulfurous gas is about 0.01% ~ 0.02%, and pH value is 3.9 ~ 4.1; Soaking water temp is 50 ~ 55 DEG C; Soak time is 40 ~ 60h.Soaking conditions should determine according to the quality of corn.Normally store corn of a specified duration and flint corn all needs stronger soaking conditions, namely require higher sulfur dioxide concentration, temperature and longer soak time.Corn is after soaking, and moisture content should reach more than 40%.
Preferably, as a kind of embodiment, in step (2), containing zymin in the process water in described technique water pot, the consumption of described zymin is 0.005% ~ 0.01% of corn gross weight, then the temperature of soaking is 30 DEG C ~ 55 DEG C, and pH is 3.0 ~ 6.0.
Preferably, as a kind of embodiment, the 3rd soak solution obtained in step (2) is back to technique water pot and recycles.
Preferably, as a kind of embodiment, in step (4), also comprise filametntary carrying out washing treatment, the washings that carrying out washing treatment obtains is mixed to the 3rd mixture.
Preferably, as a kind of embodiment, described zymin is the combination of cellulase and zytase, and the content of wherein said cellulase is 20% ~ 40% by weight, and the content of described zytase is 60% ~ 80% by weight.
Preferably, as a kind of embodiment, the content of described cellulase is 30% ± 1% by weight, and the content of described zytase is 70% ± 1% by weight.
Preferably, as a kind of embodiment, described cellulase is obtained by Li Shi Trichoderma kind, and in PH3.8 ~ 4.2, under the condition of temperature 50 C, the transformation period of described cellulase is 96 ~ 144 hours.
Preferably, as a kind of embodiment, described zytase is obtained by aspergillus niger strain, and in PH3.8 ~ 4.2, under the condition of temperature 50 C, the transformation period of described zytase is 96 ~ 144 hours.
Preferably, as a kind of embodiment, what described carrying out washing treatment adopted is all with the addition of the process water in the technique water pot of zymin.
In order to make object of the present invention, technical scheme and advantage clearly understand, below in conjunction with embodiment, the method for Production by Enzymes W-Gum of the present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.
Embodiment one
1, production unit: the production line of the wet processes W-Gum of 1200 ton per days.
2, the interpolation of zymin: add zymin (cellulase 30%, zytase 70%), addition is 0.005%.Zymin adds point for technique water pot, and continuous flow adds 30 days.In the production process of wet processes W-Gum, the conveying of raw material relies on process water conveying, and except starch washing is with except new water, other is as germ washing, and fiber wash water is process water, and process water can be recycled.
3, starch recoveries is in table one.
4, performance analysis:
Starch recovery rate improves 0.5%, i.e. voluminous uncooked amylum=1200 ton per day × 0.5%=6 ton per day.In market minimum rate 3200 yuan/ton, ton per day × 3200, direct economic benefit=6 yuan/ton=19,200 yuan/day.
Protein recovery rate improves 0.3%, and namely gold produces protein=1200 ton per day × 0.3%=3.6 ton per day.In market minimum rate 5000 yuan/ton, ton per day × 5000, direct economic benefit=3.6 yuan/ton=18,000 yuan/day.
Embodiment two
1, production unit: the production line of the wet processes W-Gum of 1200 ton per days.
2, the interpolation of zymin: add zymin (cellulase 30%, zytase 70%), addition is 0.01%.Zymin adds point for technique water pot, and continuous flow adds 30 days.In the production process of wet processes W-Gum, the conveying of raw material relies on process water conveying, and except starch washing is with except new water, other is as germ washing, and fiber wash water is process water, and process water can be recycled.
3, starch recoveries is in table one.
4, performance analysis:
Starch recovery rate improves 0.9%, i.e. voluminous uncooked amylum=1200 ton per day × 0.9%=10.8 ton per day.In market minimum rate 3200 yuan/ton, ton per day × 3200, direct economic benefit=10.8 yuan/ton=33,480 yuan/day.
Protein recovery rate improves 0.4%, and namely gold produces protein=1200 ton per day × 0.4%=4.8 ton per day.In market minimum rate 5000 yuan/ton, ton per day × 5000, direct economic benefit=4.8 yuan/ton=24,000 yuan/day.
The product yield that table one adds before and after zymin compares
| Classification (%) | Before enzyme-added | After enzyme-added (enzyme concentration 0.005%) | After enzyme-added (enzyme concentration 0.01%) |
| Starch recoveries | 69 | 69.5 | 69.9 |
| Protein yield | 4.8 | 5.1 | 5.2 |
| Fiber yield | 11 | 11.5 | 11.5 |
| Plumule yield | 10 | 10.2 | 10.4 |
| Fiber moisture | 62 | 58 | 56 |
| Plumule moisture | 60 | 57 | 55 |
| Albumen moisture | 58 | 56 | 55 |
The above embodiment only have expressed several embodiment of the present invention, and it describes comparatively concrete and detailed, but therefore can not be interpreted as the restriction to the scope of the claims of the present invention.It should be pointed out that for the person of ordinary skill of the art, without departing from the inventive concept of the premise, can also make some distortion and improvement, these all belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
Claims (10)
1. a method for Production by Enzymes W-Gum, is characterized in that: comprise the steps:
(1) corn just soaks: by corn soaking in the first soak solution, discharges soak solution, obtain the first soaking corn through first immersion treatment and the second soak solution, become corn steep liquor after described second soak solution evaporation concentration when moisture in corn is greater than 40%; The concentration of described first soak solution sulfite is 0.15% ~ 0.25%;
(2) corn soaks again: adopt the process water in technique water pot to carry out immersion treatment again in the first soaking corn of described discharge soak solution, then the immersion treatment time is 5 ~ 7 hours, carries out screening process after immersion treatment; Obtain the soaking corn again through immersion treatment again and the 3rd soak solution; Containing zymin in process water in described technique water pot;
(3) broken and separated plumule: obtain the first mixture and separated plumule after the soaking corn again that step (2) obtains is carried out broken apart plumule process;
(4) fine grinding, separated fiber: the first mixture that step (3) obtains is carried out fine grinding process and obtains the second mixture, next separating treatment is carried out to the second mixture and obtain the 3rd mixture and fiber yarn;
(5) centrifugation: the 3rd mixture obtained step (4) carries out centrifuging treatment and obtains Corn Starch Blends and protein mixture;
(6) washing and dehydration: the Corn Starch Blends obtained step (5) carries out washing and processed obtains wet corn starch;
(7) dry: the W-Gum that drying treatment obtains drying is carried out to the wet corn starch that step (6) obtains.
2. method according to claim 1, is characterized in that: in step (1), and soaking temperature is 45 DEG C ~ 50 DEG C, and soak time is 40 ~ 60 hours.
3. method according to claim 1, is characterized in that: in step (2), and the consumption of described zymin is 0.005% ~ 0.01% of corn gross weight, then the temperature of soaking is 30 DEG C ~ 55 DEG C, and pH is 3.0 ~ 6.0.
4. method according to claim 1, is characterized in that: the 3rd soak solution obtained in step (2) is back to technique water pot and recycles.
5. method according to claim 1, is characterized in that: in step (4), also comprises filametntary carrying out washing treatment, and the washings that carrying out washing treatment obtains is mixed to the 3rd mixture.
6. method according to claim 3, is characterized in that: described zymin is the combination of cellulase and zytase, and the content of wherein said cellulase is 20% ~ 40% by weight, and the content of described zytase is 60% ~ 80% by weight.
7. method according to claim 6, is characterized in that: the content of described cellulase is 30% ± 1% by weight, and the content of described zytase is 70% ± 1% by weight.
8. method according to claim 6, is characterized in that: described cellulase is obtained by Li Shi Trichoderma kind, and in pH3.8 ~ 4.2, under the condition of temperature 50 C, the transformation period of described cellulase is 96 ~ 144 hours.
9. method according to claim 6, is characterized in that: described zytase is obtained by aspergillus niger strain, and in pH3.8 ~ 4.2, under the condition of temperature 50 C, the transformation period of described zytase is 96 ~ 144 hours.
10. the method according to claim 1 ~ 9 any one claim, is characterized in that: what described carrying out washing treatment adopted is all with the addition of the process water in the technique water pot of zymin.
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| CN103665171B (en) * | 2013-11-26 | 2016-05-18 | 郑州市中食农产品加工研究院 | A kind of method of corn soaking in production of corn starch process |
| CN104558213A (en) * | 2014-12-31 | 2015-04-29 | 河南飞天农业开发股份有限公司 | Soaking process for preparing corn starch |
| CN105801712B (en) * | 2016-05-19 | 2018-11-09 | 重庆市农业科学院 | A kind of corn soaking method in waxy corn starch production |
| CN106366205A (en) * | 2016-08-30 | 2017-02-01 | 广西慧投互联网金融服务有限公司 | Preparation method of corn starch milk |
| CN106749691A (en) * | 2017-02-09 | 2017-05-31 | 合肥隆扬农业科技有限公司 | A kind of corn soaking method during production of corn starch |
| CN106832009A (en) * | 2017-03-01 | 2017-06-13 | 中粮生化能源（龙江）有限公司 | A kind of method for producing cornstarch in corn wet milling production technology using cellulase preparation |
| CN107619842A (en) * | 2017-09-25 | 2018-01-23 | 山东神州翔宇科技集团有限公司 | A kind of method of Production by Enzymes cornstarch |
| CN108659136B (en) * | 2018-04-11 | 2019-07-26 | 广西壮族自治区农业科学院农产品加工研究所 | A kind of preparation process of high-efficiency low-toxicity hair sweet potato starch |
| CN109234184B (en) * | 2018-06-24 | 2020-04-28 | 山东省科学院生物研究所 | Direct-throwing microbial agent and application thereof in wet corn soaking |
| CN109456413B (en) * | 2018-11-13 | 2021-06-01 | 秦皇岛鹏远淀粉有限公司 | Preparation method of corn starch |
| CN109651516A (en) * | 2018-12-21 | 2019-04-19 | 北安象屿金谷生化科技有限公司 | A kind of dolantin Asia raw material cornstarch processing technology |
| CN111728134B (en) * | 2020-05-19 | 2023-10-20 | 新疆家瑞泽智慧农业科技开发有限公司 | High-cellulose high-nutrition fermented corn noodles and preparation method thereof |
| US20230227585A1 (en) * | 2020-07-09 | 2023-07-20 | Novozymes A/S | Improved fiber-washing in corn wet-milling |
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Inventor after: Tong Yi Inventor after: Ding Shaoming Inventor after: Yu Feng Inventor before: He Xinmin Inventor before: Yu Feng |