CN109576324A - A kind of astragalus polyose and its biological extraction method - Google Patents
A kind of astragalus polyose and its biological extraction method Download PDFInfo
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- C12P19/00—Preparation of compounds containing saccharide radicals
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Abstract
The present invention relates to a kind of astragalus polyose and its biological extraction method, the biology extraction method includes: by Radix Astragali grinding and sieving, and three kinds of addition cellulase, hemicellulase and pectase group synthase are digested, then decocted again, merges decoction liquor；And be inoculated with bacillus subtilis in the dregs of a decoction after decoction and ferment with lactic acid bacteria, it is decocted again after fermentation, merges all decoction liquors, be drying to obtain astragalus polyose after the cleaning of 95% ethyl alcohol is added after concentration alcohol precipitation.This method is greatly improved the yield of astragalus polyose, utilize the single-minded high efficiency of enzyme and the biological nature of biofermentation, the extraction of Radix Astragali is maximized, pollution caused by the discharge such as soda acid organic solvent is avoided simultaneously, the last remaining dregs of a decoction can be used as organic fertilizer material, realize the environmentally protective of production process.
Description
Technical field
The present invention relates to technical field of polysaccharide extraction, and in particular to a kind of astragalus polyose and its biological extraction method.
Background technique
Radix Astragali is the dry root of astragalus mongholicus or astragalus membranaceus of leguminous plant.Principle active component has polysaccharide, flavones, soap
Glycosides, amino acid etc. have solid gas rising Yang, inducing diuresis for removing edema, pus draining and toxin expelling and other effects.Astragalus polyose contained therein by galactolipin,
Glucose, rhamnose, arabinose, glucuronic acid and galacturonic acid etc. are polymerized.Now studies have shown that astragalus polyose
With enhancing immunity of organisms, adjust the performances such as blood glucose, anti-aging.
Cellulose be by glucose group at macromolecular polysaccharide, be usually incorporated in one with hemicellulose, pectin, lignin
It rises, plant cell wall is made of cellulose, hemicellulose, lignin etc., and wherein principal structural component is cellulose, and cellulose squeezes
It is not soluble in water, and general organic solvent is not dissolved in, under normal temperature condition, Nature comparison is stablized, and the active principle in plant is big
All it is present in cell wall, since the presence of cellulose substances considerably increases extraction rate and recovery rate.Cellulase can
Cellulosic molecule is acted on, which can be digested and be discharged for small-molecule substance to destroy plant cell wall composition
The effective component wrapped up by cell wall accelerates the process extracted.But due to the complexity of this body structure of plant, enzymatic hydrolysis is only capable of again
To a certain extent improve effective component recovery rate, still can some can remain in the dregs of a decoction, then carry out profitable strain without
Aerobe fermentation, second extraction, while being by dregs of a decoction fermentation can feeding feed stuff.Chinese patent CN 107698692A discloses a kind of Huang
The extracting method of astragalus polysaccharides is concentrated alcohol precipitation by ultrasonic extraction after hot bath heating, highly basic processing and obtains astragalus polyose.It is Chinese special
Sharp CN 105693879A discloses a kind of extracting method of astragalus polyose, by the way that, through high pressure explosion, assisting ultrasonic is shaken after enzymatic hydrolysis
It swings, prepares astragalus polyose.
Existing frequently-used astragalus polyose extracting method alkali alcohol deposition method, buck extraction method, ultrasonic extraction and high pressure, microwave mention
It follows the example of, the extracted amount of astragalus polyose can be improved to a certain extent, but the use of soda acid not only improves extraction cost, also
It will increase environmental pressure, subsequent processing steps are cumbersome, and latter two method has special requirement to instrument and equipment, and extraction process energy consumption increases
Add.
Summary of the invention
The purpose of the present invention is to provide a kind of modes combined using zymotechnic and fermentation technique, extract from Radix Astragali
The astragalus polyose that the biological extraction method and this method of polysaccharide are extracted.
Cellulase is added, three kinds of group synthase of hemicellulase and pectase carry out in Radix Astragali grinding and sieving by the present invention
Enzymatic hydrolysis, is then decocted again, merges decoction liquor；And in the dregs of a decoction after decoction, be inoculated with bacillus subtilis and lactic acid bacteria into
It goes and ferments, decocted again after fermentation, merge all decoction liquors, be concentrated after alcohol precipitation plus be drying to obtain Radix Astragali after washes of absolute alcohol and is more
Sugar.
The dregs of a decoction obtained by the above method can be used as organic fertilizer for broiler breeding, and this method is greatly improved astragalus polyose
Yield is maximized the extraction of Radix Astragali using the single-minded high efficiency of enzyme and the biological nature of biofermentation, while avoiding soda acid
Organic solvent etc. pollutes caused by discharging, and the last remaining dregs of a decoction can be used as organic fertilizer material, realizes the green of production process
Environmental protection.
Specifically, biology extraction method of the present invention specifically comprises the following steps:
1) smashed Radix Astragali is first digested using group synthase, then is handled through decoction, separate decoction liquor and the dregs of a decoction；
Wherein, described group of synthase is the combination of cellulase, hemicellulase and pectase；
2) using the dregs of a decoction as fermentation substrate, tunning is obtained after bacillus subtilis and lactobacillus-fermented, then pass through
Decoction processing, collects decoction liquor；
3) decoction liquor for merging step 1) and step 2), is separated to obtain the astragalus polyose.
Preferably, after the Radix Astragali crushes, 30~50 meshes is crossed, are then digested again.
In the prior art, most to carry out enzymolysis processing using a kind of biological enzyme, then often obtained polysaccharide is less,
And ingredient is single.The present invention has found to digest using a group synthase by research, can effectively obtain that nutritional ingredient is uniform, yield is high
Astragalus polyose.Also, the application, can be maximum by showing the amount ratio of three on the basis of using group synthase
Guarantee its said effect.
Cellulase, hemicellulase and the pectase that described group of synthase is mass ratio 1:1:2~2:2:1；
Preferably, cellulase, hemicellulase and pectase that described group of synthase is mass ratio 2:2:1.
And it is 150~250:1 that the present invention, which further defines the Radix Astragali and the weight ratio of the cellulase,；When described
When weight ratio is 200:1, the effect of enzymatic hydrolysis is more prominent.
In order to advanced optimize hydrolysis result, the present invention is digested using following condition；Specifically: by the Radix Astragali and
Described group of synthase add to pH value be 4.5~5.5 buffer in, 45~55 DEG C at a temperature of, digest 1~3h；
Wherein, the Radix Astragali is added in the buffer in the ratio that solid-liquid ratio is 1:1.5~1:5 (g/ml).
Preferably, the Radix Astragali is added in the buffer in the ratio that solid-liquid ratio is 1:3.
Preferably, the pH value of the buffer is 4.5~5, most preferably 4.8.It is preferred that being buffered in the following way
Liquid, specifically: 4.8 ± 0.053g of citric acid, sodium citrate 7.94g, constant volume to 1000ml.
Preferably, the time of the enzymatic hydrolysis is 1.5~2.5h, most preferably 2h.
The biological extraction method of zymolysis technique and fermentation technique that the present invention uses combined, on the basis of optimization enzymatic hydrolysis
On, the dregs of a decoction obtained targetedly use bacillus subtilis and combining for lactic acid bacteria to carry out at fermentation after the enzymatic hydrolysis
Reason.Wherein, the inoculative proportion of the bacillus subtilis and lactic acid bacteria is 1:0.5~2；
Preferably, the inoculative proportion is 1:1.5~2, most preferably 1:2；
Bacillus subtilis and lactic acid bacteria of the present invention obtain in such a way that this field is conventional；It is preferred that using as follows
Mode:
Bacillus subtilis strain is seeded in MNB fluid nutrient medium, 100~200r/min shaking table, 32~38 DEG C are placed in
Under, 20~30h is cultivated, then diluting conciliation bacterial concentration with sterile saline is 10^7cfu/ml.
By lactobacillus inoculum in MRS fluid nutrient medium, it is placed in 100~200r/min shaking table, at 35~40 DEG C, culture 40
~55h, diluting conciliation bacterial concentration with sterile saline is 10^7cfu/ml.
Wherein, when the inoculum concentration of the bacillus subtilis and lactic acid bacteria is respectively 3%~6%, 3%~6%, institute's needle
Pair the specific dregs of a decoction, ferment obtain tunning in, polyoses content is considerable.Particularly preferably, the bacillus subtilis and
The inoculum concentration of lactic acid bacteria is 3%, 6%.
In order to advanced optimize ferment effect, the present invention uses following fermentation condition: by the dregs of a decoction be dried to moisture for
40%~50%, it is inoculated with the bacillus subtilis and lactic acid bacteria；It is subsequently placed in 28 DEG C~38 DEG C of temperature, closed shaking table training
3~5d is supported, tunning is obtained；
Preferably, the inoculative proportion of the bacillus subtilis and lactic acid bacteria is 1:2, and fermentation temperature is 35 DEG C, closed to shake
Bed incubation time is 4d.
Preferably, the dregs of a decoction drying moisture content is 45%.
The present invention after enzymatic hydrolysis and sends out to guarantee the utilizability and the higher astragalus polyose of purity of the subsequent dregs of a decoction
It is handled after ferment using decoction.
In step 1), the decoction processing specifically: after the reaction solution inactivation treatment after enzymatic hydrolysis, in mass, be divided to two
The secondary water that 6 times of amounts for being equivalent to the Radix Astragali are added decocts 0.5~1h, and filtering merges decoction liquor；
In step 2), the decoction processing specifically: in mass, be equivalent to being added in two portions in the tunning
The water of 6 times of Radix Astragali amounts decocts 0.5~1h, and filtering merges decoction liquor.
After the present invention merges the decoction liquor of two steps, further progress purification processes；In order to cooperate biology of the invention to extract
Method is purified by the way of alcohol precipitation.
Currently preferred, the decoction liquor merges, and conventional method can be used and obtain the astragalus polyose.The present invention is preferred
Using being first concentrated, then alcohol precipitation.
The alcohol precipitation is carried out using this field usual manner, particularly preferably in the following way: the alcohol precipitation specifically: will
Concentrate and 95% ethyl alcohol according to volume ratio 1:3 (V/V) ratio after mixing, stand for 24 hours, 3000r/min centrifugation repeats
The step is three times to get astragalus polyose.
The present invention provides a kind of prioritization scheme on the basis of optimizing each step and condition, and the biology extraction method includes
Following steps:
1) buffer that pH value is 4.5~5.5 is added in the ratio that smashed Radix Astragali is 1:1.5~1:5 in solid-liquid ratio
In, add described group of synthase, 45~55 DEG C at a temperature of, digest 1~3h；It is handled again through decoction, separates decoction liquor and the dregs of a decoction,
Collect the decoction liquor；
Wherein, the combination of described group of synthase is mass ratio 2:2:1 cellulase, hemicellulase and pectase；It is described
The weight ratio of Radix Astragali and the cellulase is 200:1.
2) dregs of a decoction are dried to moisture content is 40%~50%, then by described by bacillus subtilis and lactic acid bacteria
It is inoculated with by 3%, 6% inoculum concentration；It is subsequently placed in 28 DEG C~38 DEG C of temperature, closed 3~5d of shaking table culture is sent out
Ferment product；
3) merge the decoction liquor of step 1) and step 2), concentration, alcohol precipitation is to get the astragalus polyose.
The present invention provides a kind of methods for extracting astragalus polyose, combine extraction Radix Astragali more using zymotechnic and fermentation technique
Sugar, action condition temperature release the polysaccharide in Radix Astragali maximumlly, and obtained polysaccharide yield is high, active good, production process
Addition soda acid and special installation are not needed, it is simple and easy, it is easy to operate, and the dregs of a decoction after finally fermentation extraction polysaccharide can be direct
For feeding, reduces the subsequent processing work of the dregs of a decoction, while fully and rationally using resource, protect environment, reach green
The purpose of production.
Specific embodiment
The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..
The bacillus subtilis and lactic acid bacteria used in following examples obtains in the following way: by bacillus subtilis
It kind is seeded in MNB fluid nutrient medium, is placed in 100~200r/min shaking table, at 32~38 DEG C, cultivates 20~30h, then use nothing
It is 10^7cfu/ml that bacterium normal saline dilution, which reconciles bacterial concentration,.
By lactobacillus inoculum in MRS fluid nutrient medium, it is placed in 100~200r/min shaking table, at 35~40 DEG C, culture 40
~55h, diluting conciliation bacterial concentration with sterile saline is 10^7cfu/ml.
Buffer used in following examples: 4.8 ± 0.053g of citric acid, sodium citrate 7.94g are settled to
1000ml。
Embodiment 1
The present embodiment provides a kind of biological extraction methods of astragalus polyose, include the following steps:
1) smashed Radix Astragali is crossed into 40 meshes, the ratio for being 1:3 in solid-liquid ratio is added in the buffer that pH value is 4.8,
Be added cellulase, hemicellulase and pectase group synthase, 50 DEG C at a temperature of, digest 2h；
Wherein, the additive amount of the cellulase, hemicellulase and pectase be respectively the astragalus weight 5 ‰,
5‰,2.5‰；
2) by 100 DEG C of inactivation 15min of reaction solution after enzymatic hydrolysis, in two times plus 6 times of amount water of the Radix Astragali decoct 0.5h, mistake
Filter obtains decoction liquor and the dregs of a decoction, merges decoction liquor, and decoction liquor concentration sealing cryo-conservation is stand-by；
3) dregs of a decoction are dried to moisture content is 45%, then presses bacillus subtilis and lactic acid bacteria respectively for described
3%, 6% inoculum concentration is inoculated with；It is subsequently placed in 35 DEG C of temperature, closed shaking table culture 4d, obtains tunning；
4) 6 times of amount water of the Radix Astragali are added to decoct 0.5~1h in two times the tunning, filtering merges decoction liquor,
Concentration is stand-by；
5) decoction liquor is added the decoction liquor after merging step 2) and step 4) concentration in the ratio of 1:3 (V/V)
95% ethyl alcohol carries out alcohol precipitation, is centrifuged after standing overnight, and repeats this step 2~3 time, is drying to obtain astragalus polyose.
It after polysaccharide is completely dissolved with water, is detected using Phenol sulfuric acid procedure, the yield of the astragalus polyose is 30.71%.
Embodiment 2
The present embodiment provides a kind of biological extraction methods of astragalus polyose, include the following steps:
1) smashed Radix Astragali is crossed into 40 meshes, the buffer that pH value is 4.5 is added in the ratio for being 1:1.5 in solid-liquid ratio
In, be added cellulase, hemicellulase and pectase group synthase, 55 DEG C at a temperature of, digest 1h；
Wherein, the additive amount of the cellulase, hemicellulase and pectase is respectively the astragalus weight
7.5‰,5‰,2.5‰；
2) by 100 DEG C of inactivation 15min of reaction solution after enzymatic hydrolysis, in two times plus 6 times of amount water of the Radix Astragali decoct 0.5h, mistake
Filter obtains decoction liquor and the dregs of a decoction, merges decoction liquor, and decoction liquor concentration sealing cryo-conservation is stand-by；
3) dregs of a decoction are dried to moisture content is 45%, then presses bacillus subtilis and lactic acid bacteria respectively for described
6%, 3% inoculum concentration is inoculated with；It is subsequently placed in 28 DEG C of temperature, closed shaking table culture 5d, obtains tunning；
4) 6 times of amount water of the Radix Astragali are added to decoct 0.5~1h in two times the tunning, filtering merges decoction liquor,
Concentration is stand-by；
5) decoction liquor is added the decoction liquor after merging step 2) and step 4) concentration in the ratio of 1:3 (V/V)
95% ethyl alcohol carries out alcohol precipitation, is centrifuged after standing overnight, and repeats this step 2~3 time, is drying to obtain astragalus polyose.
It after polysaccharide is completely dissolved with water, is detected using Phenol sulfuric acid procedure, the yield of the astragalus polyose is 28.65%.
Embodiment 3
The present embodiment provides a kind of biological extraction methods of astragalus polyose, include the following steps:
1) smashed Radix Astragali is crossed into 40 meshes, the ratio for being 1:5 in solid-liquid ratio is added in the buffer that pH value is 5.5,
Be added cellulase, hemicellulase and pectase group synthase, 45 DEG C at a temperature of, digest 3h；
Wherein, the additive amount of the cellulase, hemicellulase and pectase is respectively the astragalus weight
2.5‰,2.5‰,5‰；
2) by 100 DEG C of inactivation 15min of reaction solution after enzymatic hydrolysis, in two times plus 6 times of amount water of the Radix Astragali decoct 0.5h, mistake
Filter obtains decoction liquor and the dregs of a decoction, merges decoction liquor, and decoction liquor concentration sealing cryo-conservation is stand-by；
3) dregs of a decoction are dried to moisture content is 45%, then presses bacillus subtilis and lactic acid bacteria respectively for described
4%, 4% inoculum concentration is inoculated with；It is subsequently placed in 38 DEG C of temperature, closed shaking table culture 3d, obtains tunning；
4) 6 times of amount water of the Radix Astragali are added to decoct 0.5~1h in two times the tunning, filtering merges decoction liquor,
Concentration is stand-by；
5) decoction liquor is added the decoction liquor after merging step 2) and step 4) concentration in the ratio of 1:3 (V/V)
95% ethyl alcohol carries out alcohol precipitation, is centrifuged after standing overnight, and repeats this step 2~3 time, is drying to obtain astragalus polyose.
It after polysaccharide is completely dissolved with water, is detected using Phenol sulfuric acid procedure, the yield of the astragalus polyose is 29.10%.
Comparative example 1
This comparative example provides a kind of biological extraction method of astragalus polyose, and the difference with embodiment 1 is only that, group synthase replacement
For " cellulase list enzyme ".
1) smashed Radix Astragali is crossed into 40 meshes, the ratio for being 1:3 in solid-liquid ratio is added in the buffer that pH value is 4.8,
Be added cellulase, hemicellulase and pectase group synthase, 50 DEG C at a temperature of, digest 2h；
Wherein, the cellulase additive amount is the 5 ‰ of the astragalus weight；
2) by 100 DEG C of inactivation 15min of reaction solution after enzymatic hydrolysis, in two times plus 6 times of amount water of the Radix Astragali decoct 0.5h, mistake
Filter obtains decoction liquor and the dregs of a decoction, merges decoction liquor, and decoction liquor concentration sealing cryo-conservation is stand-by；
3) dregs of a decoction are dried to moisture content is 45%, then presses bacillus subtilis and lactic acid bacteria respectively for described
3%, 6% inoculum concentration is inoculated with；It is subsequently placed in 35 DEG C of temperature, closed shaking table culture 4d, obtains tunning；
4) 6 times of amount water of the Radix Astragali are added to decoct 0.5~1h in two times the tunning, filtering merges decoction liquor,
Concentration is stand-by；
5) decoction liquor is added the decoction liquor after merging step 2) and step 4) concentration in the ratio of 1:3 (V/V)
95% ethyl alcohol carries out alcohol precipitation, is centrifuged after standing overnight, and repeats this step 2~3 time, is drying to obtain astragalus polyose.
It after polysaccharide is completely dissolved with water, is detected using Phenol sulfuric acid procedure, the yield of the astragalus polyose is 25.18%.
Comparative example 2
This comparative example provides a kind of biological extraction method of astragalus polyose, the difference from embodiment 1 is that, only use enzyme digestion reaction
After extract,
1) smashed Radix Astragali is crossed into 40 meshes, the ratio for being 1:3 in solid-liquid ratio is added in the buffer that pH value is 4.8,
Be added cellulase, hemicellulase and pectase group synthase, 50 DEG C at a temperature of, digest 2h；
Wherein, the additive amount of the cellulase, hemicellulase and pectase be respectively the astragalus weight 5 ‰,
5‰,2.5‰；
2) by 100 DEG C of inactivation 15min of reaction solution after enzymatic hydrolysis, in two times plus 6 times of amount water of the Radix Astragali decoct 0.5h, mistake
Filter obtains decoction liquor and the dregs of a decoction, merges decoction liquor,
5) decoction liquor is added to 95% ethyl alcohol in the ratio of 1:3 (V/V), alcohol precipitation is carried out, is centrifuged after standing overnight,
This step 2~3 time are repeated, astragalus polyose is drying to obtain.
It after polysaccharide is completely dissolved with water, is detected using Phenol sulfuric acid procedure, the yield of the astragalus polyose is 22.37%.
Although above having used general explanation, specific embodiment and test, the present invention is made to retouch in detail
It states, but on the basis of the present invention, it can be made some modifications or improvements, this is apparent to those skilled in the art
's.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, belong to claimed
Range.
Claims (10)
1. a kind of biological extraction method of astragalus polyose, which comprises the steps of:
1) smashed Radix Astragali is first digested using group synthase, then is handled through decoction, separate decoction liquor and the dregs of a decoction；
Wherein, described group of synthase is the combination of cellulase, hemicellulase and pectase；
2) using the dregs of a decoction as fermentation substrate, tunning is obtained after bacillus subtilis and lactobacillus-fermented, then through decocting
Decoction liquor is collected in processing；
3) merge decoction liquor obtained by step 1) and step 2), separated to obtain astragalus polyose.
2. biology extraction method according to claim 1, which is characterized in that described group of synthase is mass ratio 1:1:2~2:2:1
Cellulase, hemicellulase and pectase；
Preferably, cellulase, hemicellulase and pectase that described group of synthase is mass ratio 2:2:1.
3. biology extraction method according to claim 1 or 2, which is characterized in that the weight of the Radix Astragali and the cellulase
Amount is than being 150~250:1；
Preferably, the weight ratio is 200:1.
4. described in any item biological extraction methods according to claim 1~3, which is characterized in that the enzymatic hydrolysis specifically: will be described
Radix Astragali and described group of synthase add to pH value be 4.5~5.5 buffer in, 45~55 DEG C at a temperature of, digest 1~3h；
Wherein, the Radix Astragali is added in the buffer in the ratio that solid-liquid ratio is 1:1.5~1:5；
Preferably, the enzymatic hydrolysis specifically: add to the Radix Astragali and described group of synthase in the buffer that pH value is 4.8,45
At a temperature of~55 DEG C, 2h is digested；
Wherein, the Radix Astragali is added in the buffer in the ratio that solid-liquid ratio is 1:3.
5. biology extraction method according to any one of claims 1 to 4, which is characterized in that the bacillus subtilis and cream
The inoculative proportion of sour bacterium is 1:0.5~2；
Preferably, the inoculative proportion is 1:2.
6. described in any item biological extraction methods according to claim 1~5, which is characterized in that the bacillus subtilis and cream
The inoculum concentration of sour bacterium is respectively 3%~6%, 3%~6%；
Preferably, the inoculum concentration of the bacillus subtilis and lactic acid bacteria difference 3%, 6%.
7. described in any item biological extraction methods according to claim 1~6, which is characterized in that fermentation described in step 2) is specific
Are as follows: it is 40%~50% that the dregs of a decoction, which are dried to moisture, inoculates the bacillus subtilis and lactic acid bacteria；It is subsequently placed in 28
DEG C~38 DEG C of temperature in, closed 3~5d of shaking table culture obtains tunning.
8. described in any item biological extraction methods according to claim 1~7, which is characterized in that in step 1), the decoction processing
Specifically: after the reaction solution inactivation treatment after enzymatic hydrolysis, in mass, it is added thereto is equivalent to 6 times of the Radix Astragali in two times
The water of amount decocts 0.5~1h, and filtering merges decoction liquor；
And/or in step 2), the decoction processing specifically: in mass, be added in two portions in Xiang Suoshu tunning quite
0.5~1h is decocted in the water that 6 times of the Radix Astragali are measured, filtering merges decoction liquor.
9. described in any item biological extraction methods according to claim 1~8, which comprises the steps of:
1) smashed Radix Astragali is added in the buffer that pH value is 4.5~5.5 in the ratio that solid-liquid ratio is 1:1.5~1:5, is added
Add described group of synthase, 45~55 DEG C at a temperature of, digest 1~3h；It is handled again through decoction, separates decoction liquor and the dregs of a decoction；
Wherein, the combination of described group of synthase is mass ratio 2:2:1 cellulase, hemicellulase and pectase；The Radix Astragali
Weight ratio with the cellulase is 200:1；
2) by the dregs of a decoction be dried to moisture content be 40%~50%, then by 3%, 6% inoculum concentration be inoculated with thereto described in
Bacillus subtilis and lactic acid bacteria；It is subsequently placed in 28 DEG C~35 DEG C of temperature, closed 3~5d of shaking table culture, obtains fermentation and produce
Object；
3) merge decoction liquor obtained by step 1) and step 2), concentration, alcohol precipitation is to get the astragalus polyose；
Preferably, the alcohol precipitation specifically: by concentrate and 95% ethyl alcohol according to volume ratio 1:3 ratio after mixing,
It stands for 24 hours, 3000r/min centrifugation repeats the step three times to get astragalus polyose.
10. astragalus polyose made from biology extraction method according to any one of claims 1 to 9.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111304266A (en) * | 2019-12-12 | 2020-06-19 | 武汉新华扬生物股份有限公司 | Biological enzymolysis fermentation process for aloe |
| CN111671790A (en) * | 2020-07-10 | 2020-09-18 | 武倩 | Chinese herbal medicine micro-molecule fermentation extraction process |
| CN113773985A (en) * | 2021-08-20 | 2021-12-10 | 江南大学 | Bacillus subtilis for improving traditional Chinese medicine polysaccharide yield and regulating skin barrier and immunity |
| CN114606287A (en) * | 2022-04-22 | 2022-06-10 | 乔金星 | Method for improving extraction amount of polysaccharide and flavone from dogbane leaves and fermentation tank thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1994004162A1 (en) * | 1988-05-23 | 1994-03-03 | Verbiscar Anthony J | Astragalus polysaccharide immunomodulators |
| CN1510143A (en) * | 2002-12-24 | 2004-07-07 | 中国农业大学 | Extraction of milk vetch polysaccharide by enzyme treatment |
| CN103540629A (en) * | 2013-09-29 | 2014-01-29 | 河南牧翔动物药业有限公司 | Method for extracting astragalus polysaccharide through microorganism Asp-1 fermentation method |
| CN104367987A (en) * | 2014-12-08 | 2015-02-25 | 河南牧翔动物药业有限公司 | Astragalus mongholicus formulation for veterinary use and preparation method thereof |
| CN105420144A (en) * | 2015-11-03 | 2016-03-23 | 许昌市天源生物科技股份有限公司 | Method for producing astragalus polysaccharide through acetobacter orientalis |
| CN106317236A (en) * | 2015-07-02 | 2017-01-11 | 瑞普（天津）生物药业有限公司 | Method for increasing astragalus polysaccharides by using fermentation technology |
-
2018
- 2018-11-15 CN CN201811371751.9A patent/CN109576324A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1994004162A1 (en) * | 1988-05-23 | 1994-03-03 | Verbiscar Anthony J | Astragalus polysaccharide immunomodulators |
| CN1510143A (en) * | 2002-12-24 | 2004-07-07 | 中国农业大学 | Extraction of milk vetch polysaccharide by enzyme treatment |
| CN103540629A (en) * | 2013-09-29 | 2014-01-29 | 河南牧翔动物药业有限公司 | Method for extracting astragalus polysaccharide through microorganism Asp-1 fermentation method |
| CN104367987A (en) * | 2014-12-08 | 2015-02-25 | 河南牧翔动物药业有限公司 | Astragalus mongholicus formulation for veterinary use and preparation method thereof |
| CN106317236A (en) * | 2015-07-02 | 2017-01-11 | 瑞普（天津）生物药业有限公司 | Method for increasing astragalus polysaccharides by using fermentation technology |
| CN105420144A (en) * | 2015-11-03 | 2016-03-23 | 许昌市天源生物科技股份有限公司 | Method for producing astragalus polysaccharide through acetobacter orientalis |
Non-Patent Citations (1)
| Title |
|---|
| 陈雷等: "乳酸菌―芽孢菌发酵黄芪对小鼠免疫功能作用的研究", 《中国畜牧兽医》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111304266A (en) * | 2019-12-12 | 2020-06-19 | 武汉新华扬生物股份有限公司 | Biological enzymolysis fermentation process for aloe |
| CN111671790A (en) * | 2020-07-10 | 2020-09-18 | 武倩 | Chinese herbal medicine micro-molecule fermentation extraction process |
| CN113773985A (en) * | 2021-08-20 | 2021-12-10 | 江南大学 | Bacillus subtilis for improving traditional Chinese medicine polysaccharide yield and regulating skin barrier and immunity |
| CN113773985B (en) * | 2021-08-20 | 2023-08-25 | 江南大学 | Bacillus subtilis for improving yield of traditional Chinese medicine polysaccharide and regulating skin barrier and immunity |
| CN114606287A (en) * | 2022-04-22 | 2022-06-10 | 乔金星 | Method for improving extraction amount of polysaccharide and flavone from dogbane leaves and fermentation tank thereof |
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