CN103254320A - Method for producing corn starch by utilizing enzymic method - Google Patents
Method for producing corn starch by utilizing enzymic method Download PDFInfo
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Abstract
The invention provides a method for producing a corn starch by utilizing enzymic method. The method comprises the following steps: primarily soaking corns; secondarily soaking the corns; crushing and separating germs; accurately grinding; separating fibers; performing centrifugal separation; washing and dehydrating; and drying, wherein in the secondary corn soaking process, the secondary soaking treatment is performed on the primarily-soaked corns from which a soaking liquid is discharged by using process water in a process water tank; the secondary soaking treatment time is from 5 to 7 hours; and the screening treatment is performed after the soaking treatment is finished, so that the secondarily-soaked corns treated by the secondary soaking and a third soaking liquid are obtained. By performing the soaking process for two times and adding an enzymic preparation in the secondary soaking process, the structure and the physical property of corn endosperm are changed by the primary soaking treatment; and the cell walls and the testa connection fibers of corn seed grains can be promoted to be ruptured or separated by utilizing the characteristics of the enzymic preparation when the enzymic preparation is added in the secondary soaking process. In addition, the corns soaked primarily are more favorable for the function exerting of the enzymic preparation.
Description
Technical field
The invention belongs to the corn deep processing field, be specifically related to a kind of method of Production by Enzymes W-Gum.
Background technology
W-Gum is to be raw material with the corn grain, soaks the product that crushing and screening, separating, washing, dewatered drying are made by sulfurous acid.W-Gum is except being directly used in food, papermaking, weaving, medicine and other fields, and is most for deep processing.Starch industry was a bigger industry of China's corn usage quantity, and the output of China's W-Gum was rising tendency year by year from 1999 to 2011.National W-Gum amount reached 2,170 ten thousand tons in 2009, occupied the second place of the world.Dian Fentang is to be the high-grade products of raw material with the W-Gum, and higher economic value and edibleness are arranged, and is widely used in the industries such as food, medicine, chemical industry, fermentation.The Dian Fentang that W-Gum is produced comprises glucose, fructose, maltose etc.The maize treacle that the W-Gum deep processing the is made utilization that is absorbed by the body easily is the good sweeting agent of making candy, cake, beverage and can.Sorbyl alcohol is the derivative of Dian Fentang, and mainly for the production of vitamins C, domestic needs is vigorous in recent years.The modified starch that carries out modification on the basis of starch is the product that new development is in recent years got up, and is of many uses, developed more than 3000 kind abroad, and China only develops more than 50 kind.It is widely used in industry such as papermaking, weaving, food, feed, medicine, daily use chemicals, oil, the usage quantity maximum be industries such as papermaking, food and textiles, have good prospects.Therefore, domestic and international demand to W-Gum heightens in recent years.
In addition, in the W-Gum production process, except the W-Gum major product, also can obtain Semen Maydis oil, zein fiber, protein powder and three kinds of byproducts of corn steep liquor.The unsaturated fatty acid content of Semen Maydis oil reaches more than 85%, mainly contains oleic acid and linolic acid, and the human absorptivity reaches more than 97%; Contain sitosterol in the Semen Maydis oil, have the effect that cholesterol increases that suppresses; Rich in vitamin E is for human body cell division, delaying senility has certain effect.Corn dietary fiber is made up of Mierocrystalline cellulose and hemicellulose, has effect hypotensive, the prevention cardiovascular and cerebrovascular diseases.Protein powder is the significant by-products of corn wet processes, and its protein content is up to 50%～70%, and the raw material mainly as producing high protein feed also can be used to produce other Industrial products such as prolamine.Total protein content is 44%～48% (butt) in the corn steep liquor, and as feed formulation, corn steep liquor is a kind of high protein, high-octane nutrition, contains abundant vitamins B and mineral substance simultaneously.
At present, wet method processing technology is generally adopted in W-Gum processing in the world.Starch, protein, plumule, fiber etc. are main in the corn adopts mechanical means such as pulverizing, screening, centrifugal, extruding, filtration to separate, concentrate and dry.And those skilled in the art also adopts several different methods to improve the complete processing of W-Gum, for example patent publication No. is that the Chinese invention patent of 101372702A discloses corn soaking time method in a kind of shortening W-Gum production process, thereby this method is utilized high pressure and prozyme to unite to soak the time that shortens corn soaking.But this method adopts the high pressure immersion to be unfavorable for especially using in industrial production, this method complex process, and its equipment is the input cost height again, and the tooling cost height, and energy consumption is big, shortens the corn soaking time with this, and operability is not strong in the reality.In addition, patent publication No. is that the Chinese invention patent of CN1831013A also discloses a kind of W-Gum enzyme process immersion production method, add the lactobacillus inoculation through fermentation culture in the immersion process of this method the first step in the corn steep liquor, in the corn steep liquor of the 3rd step fragmentation, add plant protease and discharge starch with abundant degrade proteins matrix, and then further shorten soak time.Disclosed method all is to add proteolytic enzyme in soak solution in above-mentioned two patents, but proteolytic enzyme can be with the partial protein hydrolysis in the W-Gum, this finally can cause the loss of the protein of the economically valuable of W-Gum production process, and be dissolved in the process water that soluble proteins in the water also can disposal of pollutants, be unfavorable for environmental protection.Therefore, separating effect and the purity of the isolate in the W-Gum production process of prior art are limited, deep processing cost for purification height, and the energy consumption of isolate sepn process (water, electricity, steam) is high, efficient is low.
Summary of the invention
Based on this, be necessary the problem at the prior art existence, a kind of method of Production by Enzymes W-Gum is provided.This method is on the basis of traditional wet method processing technology, has adopted soaking technology twice, and is adding zymin in the soaking technology again, with raising mechanical separation effect and separation efficiency, and then effectively improves isolate purity, recovery rate.In addition, adopt method of the present invention not only can improve the yield of W-Gum, also can improve the yield of protein powder, and effective energy efficient simultaneously.
Technical scheme of the present invention is as follows:
A kind of method of Production by Enzymes W-Gum comprises the steps:
(1) corn just soaks: corn soaking in first soak solution, is discharged soak solution greater than 40% the time when moisture in the corn, obtain first soaking corn and second soak solution through first immersion treatment, become corn steep liquor after the described second soak solution evaporation concentration;
(2) corn soaks again: carry out immersion treatment again in the first soaking corn of the process water in the adopting process water pot to described discharge soak solution, the immersion treatment time is 5～7 hours again, sieves processing after immersion treatment; Obtain through soaking corn again and the 3rd soak solution of immersion treatment again;
(3) fragmentation and separated plumule: the soaking corn again that step (2) is obtained carries out obtaining first mixture and separated plumule after broken apart plumule is handled;
(4) correct grinding, separated fiber: first mixture that step (3) is obtained finish grindes to handle and obtains second mixture, next second mixture is carried out separating treatment and obtains the 3rd mixture and fiber yarn;
(5) centrifugation: the 3rd mixture that step (4) is obtained carries out centrifugation to be handled and obtains W-Gum mixture and protein mixture;
(6) washing and dehydration: the W-Gum mixture that step (5) is obtained washs with processed and obtains wet corn starch;
(7) drying: the wet corn starch that step (6) is obtained carries out the W-Gum that drying treatment obtains drying.
Among embodiment, in the step (1), the concentration of sulfurous acid is 0.15%～0.25% in described first soak solution therein, and soaking temperature is 45 ℃～50 ℃, and soak time is 40～60 hours.
Among embodiment, in the step (2), contain zymin in the process water in the described technology water pot therein, described enzyme preparation amount is 0.005%～0.01% of corn gross weight, and the temperature of Jin Paoing is 30 ℃～55 ℃ again, and pH is 3.0～6.0.
Among embodiment, the 3rd soak solution that obtains in the step (2) is back to the technology water pot and recycles therein.
Among embodiment, in the step (4), comprise that also to filametntary carrying out washing treatment, the washings that carrying out washing treatment obtains is mixed to the 3rd mixture therein.
Among embodiment, described zymin is the combination of cellulase and zytase therein, and the content of wherein said cellulase is 20%～40% by weight, and the content of described zytase is 60%～80% by weight.
Among embodiment, the content of described cellulase is 30% ± 1% by weight therein, and the content of described zytase is 70% ± 1% by weight.
Among embodiment, described cellulase is to be made by Li Shi Trichoderma kind therein, and in PH3.8～4.2, under the condition that temperature is 50 ℃, the transformation period of described cellulase is 96～144 hours.
Among embodiment, described zytase is to be made by aspergillus niger strain therein, and in PH3.8～4.2, under the condition that temperature is 50 ℃, the transformation period of described zytase is 96～144 hours.
Therein among embodiment, described carrying out washing treatment adopts all is the process waters that added in the technology water pot of zymin.
The invention has the beneficial effects as follows:
1, the present invention has adopted soaking technology twice, and added zymin in the operation soaking, first immersion treatment can change structure and the physical properties of corn embryosperm again, weakens the connecting key in the protein, reduce the physical strength of corn kernel, lixiviate goes out the part soluble substance; And added the characteristic that zymin can be utilized zymin in the operation soaking again, and promoting the cell walls of corn kernel and plant skin to connect fiber and break or separates, the corn that passes through first immersion treatment simultaneously more is conducive to zymin and plays a role.
2, method of the present invention can be utilized existing wet method starch processing units, do not increase new energy consumption, improved the functioning efficiency of zymin, reduced the interpolation cost of zymin, and zymin can recycle, and has improved mechanical separation effect and separation efficiency; Reduce the bath water amount, improved detersive efficiency; Improve centrifugal efficiency, and then reduced power consumption, improved economic benefit, had great using value.
3, employing method of the present invention not only can improve the yield of W-Gum, also can improve the yield of protein powder.Because the present invention does not add proteolytic enzyme, can avoid protein dissolving, reduced the loss of the protein of the economically valuable of W-Gum production process, reduced the pollution of soluble proteins in the discharge water.
4, the present invention adopts the content of coenzyme proteolytic enzyme in the cellulase that Li Shi Trichoderma kind makes low, therefore avoid enzymolysis to increase solvend, so reduced sewage discharge, be more conducive to environmental protection, and the minimizing of the solvend of enzymolysis also is conducive to improve separating effect and product purity.
5, zymin long half time of the present invention makes process water sustainable playing a role in recycling of containing zymin, and has reduced the input cost of zymin.
6, the enzyme described in the present invention is cellulase or zytase or the combination of the two.The present invention utilizes cellulase and hemicellulase and to plant skin with the cell walls in the corn kernel to connect the characteristic that fiber breaks or separates, and makes the very fast decomposition of plant cell wall or collapse make the Mierocrystalline cellulose in the corn kernel, effective decomposition of hemicellulose can promote materials such as kind of a skin, endosperm starch, protein, plumule, fiber effectively to separate.The present invention also utilizes zytase can reduce the wetting ability of Mierocrystalline cellulose and hemicellulose to greatest extent simultaneously, therefore, can reduce the fibrous matter water content, concentrate and drying efficiency so improved, reduced to concentrate with dry and used quantity of steam, and then greatly saved energy consumption.
Description of drawings
Below in conjunction with concrete drawings and the specific embodiments, the present invention is further elaborated.
Fig. 1 is the schematic flow sheet of the method for Production by Enzymes W-Gum of the present invention.
Embodiment
Described zymin is the combination of cellulase or zytase or cellulase and zytase in the present invention.Cellulase of the present invention and zytase all are Baiyin Sino Biotechnology Co., Ltd. carries out fermentative production according to the bacterial classification survival temperature of Chinese industrial microbial strains preservation administrative center record products.
The concrete preparation method of described cellulase is as follows:
Raw material: Chinese industrial microbial strains preservation administrative center provides Li Shi Trichoderma kind (numbering 13051)
The preparation method:
1. slant strains preparation: wood mould (13051) strain bacterial classification is transferred to the PDA medium slant under aseptic condition, cultivated 1～2 day down at 30 ℃, treat that the inclined-plane covers with to take out that to be put in 4 ℃ of refrigerations standby.
2. the preparation on eggplant bottle inclined-plane: slant strains is transferred to PDA eggplant bottle inclined-plane under aseptic condition, cultivated 1～2 day down at 30 ℃, treat that eggplant bottle inclined-plane covers with to take out that to be put in 4 ℃ of refrigerations standby.
3. the preparation of spore suspension: with the ratio of eggplant bottle inclined-plane with inoculum size 6%, under aseptic condition, change in the sterilized water of 5 liters of triangular flasks, the spore suspension in the triangular flask is changed under aseptic condition in 5 liters of inoculation bottles.
4. seed enlarged culturing: stalk, wheat bran and inorganic salt etc. are dropped in the seeding tank of 6m3 according to a certain percentage, with the tap water constant volume to 3m3, stir, behind the steam sterilizing, treat to insert when temperature drops to 30～32 ℃ spore suspension, than being 1:0.2～0.5, temperature is 30～32 ℃ at air quantity, tank pressure is to cultivate 28～36h under 0.05～0.07Mpa condition, gets seed enlarged culturing kind liquid.
5. liquid submerged fermentation: stalk, wheat bran and inorganic salt etc. are dropped in the fermentor tank of 80m3 according to a certain percentage, with the tap water constant volume to 50m3, stir, behind the steam sterilizing, seed enlarged culturing kind liquid when treating that temperature drops to 30～32 ℃, than being 1:0.2～0.5, temperature is 30～32 ℃ at air quantity, tank pressure is to cultivate 98～110h under 0.05～0.08Mpa condition, gets fermentation liquid.
6. the purification of cellulase: utilize polypropylene sheet frame filter that fermentation liquid is carried out solid-liquid separation, obtain filter residue and cellulase clear liquid, the cellulase clear liquid adopts hollow-fibre membrane (molecular weight 10000 dalton) to carry out about 7 times of ultrafiltration and concentration, and it is to be dried to squeeze into material-storage jar.
7. dry: the cellulase solution that will concentrate adopts pressure type drying tower spraying drying, 125～135 ℃ of inlet temperatures, and 60～70 ℃ of temperature outs obtain the Solka-floc enzyme.
8. stdn: carriers such as Solka-floc enzyme adding starch are re-dubbed required product.
9. finished product: with composite good product quantitative package, warehouse-in.
The concrete preparation method of described zytase is as follows:
Raw material: Chinese industrial microbial strains preservation administrative center provides aspergillus niger strain (numbering 40613)
The preparation method:
1. slant strains preparation: aspergillus niger (40613) strain bacterial classification is transferred to the PDA medium slant under aseptic condition, cultivated 1～2 day down at 30 ℃, treat that the inclined-plane covers with to take out that to be put in 4 ℃ of refrigerations standby.
2. the preparation of seed suspension: (lactose, wheat bran and inorganic salt etc. add according to a certain percentage to change slant strains over to prepare liquid nutrient medium 5 liters of triangle culturing bottles under aseptic condition, PH5.0～5.8, sterilization) in, at shaking speed 180rpm～200rpm, cultivate 36h under 29～32 ℃ of conditions, it is standby to get zytase seed suspension.
3. seed enlarged culturing: lactose, wheat bran and inorganic salt etc. are dropped in the seeding tank of 6m3 according to a certain percentage, with the tap water constant volume to 3m3, stir, behind the steam sterilizing, treat to insert when temperature drops to 29～32 ℃ zytase seed suspension, be 1:1～1.2 at the air quantity ratio, temperature is 29～32 ℃, tank pressure is 0.05～0.07Mpa, cultivates 32～36h under rotating speed 180rpm～300rpm condition, gets seed enlarged culturing kind liquid.
4. liquid submerged fermentation: lactose, wheat bran and inorganic salt etc. are dropped in the fermentor tank of 80m3 according to a certain percentage, with the tap water constant volume to 50m3, stir, behind the steam sterilizing, treat that temperature changes seed enlarged culturing kind liquid over to when dropping to 30～32 ℃, be 1:1～1.2 at the air quantity ratio, temperature is 29～32 ℃, tank pressure is 0.05～0.08Mpa, cultivates 140～150h under rotating speed 180rpm～300rpm condition, gets fermentation liquid.
5. the purification of zytase: utilize polypropylene sheet frame filter that fermentation liquid is carried out solid-liquid separation, obtain filter residue and zytase clear liquid, the zytase clear liquid adopts hollow-fibre membrane (molecular weight 10000 dalton) to carry out about 7 times of ultrafiltration and concentration, and it is to be dried to squeeze into material-storage jar.
6. dry: the zytase liquid that will concentrate adopts pressure type drying tower spraying drying, 125～135 ℃ of inlet temperatures, and 60～70 ℃ of temperature outs obtain the powdery zytase.
7. stdn: carriers such as powdery zytase adding starch are re-dubbed required product.
8. finished product: with composite good product quantitative package, warehouse-in.
Zymin of the present invention can refer to the production stage that process water is added into W-Gum: the zymin of solid is dissolved with 5～10 times of process waters, add volume according to commodity corn processing flow rate calculation enzyme liquid stream, the addition of zymin is that 0.005-0.01%(is in commodity corn weight).For example: 10 tons/hour of commodity corns, addition 0.01% i.e. 1000 grams, and 10 times of process water dissolvings are 10 liters.The stream of enzyme liquid adds namely 10 liters/hour of volumes.
The present invention also can adopt liquid enzyme formulation directly to add in the production process of W-Gum.
As shown in Figure 1, specific operation process of the present invention is as follows:
1, raw material handling: what the present invention adopted generally is commodity corn, therefore needs corn is carried out raw material handling before handling, owing to contain various dust and weed-trifles, organic and inorganic impurity in the commodity corn.In order to guarantee safety in production and quality product, must clear up the impurity that exists in the corn.The method of cleaning corn mainly adopts screening, selection by winnowing etc.Cleaning equipment has vibratory screening apparatus, specific-gravity stoner, permanent magnetic pulley and wheat washer etc.
2, immersion treatment: the immersion of corn directly has influence on the ordinary production of later process starch, and what influence Starch Production goes out rate, quality and output.Therefore, corn soaking is one of very important operation in the W-Gum production process.The purpose of corn soaking is structure and the physicochemical property that change endosperm, weakens the connecting key in the protein, reduces the physical strength of corn kernel, and lixiviate goes out the part soluble substance, and suppresses harmful activity of the microorganism that comes in the Corn Belt.
Immersion of the present invention was divided into for two steps, earlier corn is carried out just soaking, and then adopt the process water that has added zymin that corn is soaked again, concrete technological process is: (1) corn just soaks: with corn soaking in first soak solution, when moisture in the corn is discharged soak solution greater than 40% the time, obtain corn and second soak solution through first immersion treatment, become corn steep liquor after the described second soak solution evaporation concentration; (2) corn soaks again: the process water in the adopting process water pot is to carrying out immersion treatment again in the corn of described discharge soak solution, the immersion treatment time is 5～7 hours again, sieves processing after immersion treatment; Obtain through corn and the 3rd soak solution of immersion treatment again.The present invention at first adopts and just soaks preliminary structure and the physicochemical property that change corn embryosperm, reduce the physical strength of corn kernel, lixiviate simultaneously goes out the part soluble substance, and then adopt the process water that has added zymin to soak again, because the structure through the first corn that soaks changes, therefore just soak for the zymin in soaking again provides more advantageous environment, make things convenient for zymin to play a role.
The corn soaking method is divided into static infusion method and adverse current diffusion infusion method.Static infusion method belongs to single jar of corn soaking, and namely the immersion water of each jar is not carried mutually.Adverse current diffusion infusion method also is diffusion process or multiple tank series connection counter-current.At present, most enterprises use adverse current diffusion infusion method to come soaking corn.The adverse current diffusion process is that several steeping tanks, pump and pipeline are together in series, principle according to the adverse current immersion, sulfurous acid soak water unlike static method in fresh corn is squeezed into jar, but squeeze in the corn jar that soak time is the longest, to soak water with pump after the circulation and squeeze into the corn soaking jar of short period of time immersion slightly, will soak water like this and squeeze into another jar from a jar successively against the direction of newly advancing corn.The direction that the direction that the sulfurous acid tank switching flows and corn feed intake is opposite, and just the direction that the solubility dry matter content reduces in the corn is opposite with the direction of solubility dry substance concentration raising in the immersion water.What just soak employing in the present embodiment is adverse current diffusion infusion method, adopts static infusion method and soak again, and adverse current is spread infusion method to present embodiment and static infusion method combines, and makes zymin better play a role, and improved the yield of final W-Gum.And the soak solution of Jin Paoing can be back to technology water pot continuation use again, has saved enzyme preparation amount.And the resulting soak solution of first immersion of two step infusion methods of the present invention does not contain zymin, is conducive to following process.
(3) fragmentation and separated plumule: carry out obtaining first mixture and separated plumule after broken apart plumule is handled through the corn of immersion treatment again with what step (2) obtained.
Corn is through after soaking, and variation has taken place for its physics, chemical property, and the connecting key between plumule and the endosperm weakens.Connecting key in the corn embryosperm between protein and the starch also weakens.Soak the back maize germ and contain moisture about 60%, have higher elasticity, and when fragmentation, from corn grain, separate easily.In addition, endosperm starch matter part is also worn into particle when fragmentation, and therefrom discharges 25% with interior starch.The purpose of corn fragmentation is that plumule separates with endosperm, and discharges the starch of some amount.
Soaked corn is discharged soak solution, with corn steep liquor corn is sent into sandstone earlier with 45～50 ℃ warm water and captures swirler removal sandstone, sends into gravity curved screen then, tells the fluming water reuse, and corn enters in the corn hopper in order to entering crusher in crushing.The broken general method that adopts twice fragmentation of corn, that is: corn → primary fragmentation → germ separation → secondary breaking → germ separation.Corn grain is through primary fragmentation, and most of plumule separates with endosperm, pulls plumule out by floating jar, enters secondary breaking again, and through twice fragmentation, plumule is all separated.The plumule of separating, vibratory screening apparatus by continuous interpolation the process water spray of zymin, the starch milk of flush away plumule surface adhesion, seitan etc., through the whizzer preliminary hydro-extraction to the plumule water content less than 36%.
(4) correct grinding, separated fiber: first mixture that step (3) is obtained finish grindes to handle and obtains second mixture, next second mixture is carried out separating treatment and obtains the 3rd mixture and fiber yarn.
Behind the fragmentation of corn process and the separated plumule, contain endosperm particulate, seitan, cortex and partial starch particle.Most of starch is included in endosperm particle and the cortex, must carry out meticulous grinding, and could discharge starch, protein and Mierocrystalline cellulose to greatest extent, for good condition is created in the separation of each component later on.The purpose of correct grinding is to destroy the combination of starch and non-starch component in the corn fragment, makes starch dissociate out to greatest extent, tells fibrous residue, and makes that protein separates with starch granules in the endosperm, in order to further separate and make with extra care.Fiber separation mainly is to discharge fibrous residue behind the starch through repeatedly having added the process water washing of zymin, makes it contain less free starch and in conjunction with starch.Fiber after the washing becomes dried slag crust through water squeezing, oven dry.Zymin of the present invention can reduce the wetting ability of Mierocrystalline cellulose and hemicellulose to greatest extent, therefore, can reduce the fibrous matter water content, concentrates and drying efficiency so improved, and has reduced to concentrate with dry and has used quantity of steam, and then greatly saved energy consumption.
(5) centrifugation: the 3rd mixture that step (4) is obtained carries out centrifugation to be handled and obtains W-Gum mixture and protein mixture.
It is high especially that grade non-starchy material, particularly protein content of more protein, fat, ash also contained in fecula Ruzhong behind the separated fiber, it must be separated, and just can obtain purer starch.
Being separated in the butterfly centrifugal machine of starch and protein carried out, because particle diameter and the proportion of starch granules are bigger than albumen plasmid, its settling velocity in suspension is faster than protein particulate, and available separating centrifuge makes starch and protein separation efficiently.
(6) washing and dehydration: the W-Gum mixture that step (5) is obtained washs with processed and obtains wet corn starch.
For removing solubility and insoluble protein, reduce starch acidity and improve suspension concentration, pass through ten grades of swirler countercurrent washings with new process water.Washing back starch carries out mechanical dehydration with horizontal type scraper centrifuger, require to take off to the water content of wet starch be 38%～40%.
(7) drying: the wet corn starch that step (6) is obtained carries out the W-Gum that drying treatment obtains drying.
Drying is to utilize heat energy to remove the operation sequence of moisture in the destarching.Adopt the air stream drying mode when starch is dry.The control of starch drying process condition: (1) wind speed is often selected 17～20m for use generally at 14～24m/s, and wind speed is low excessively, and the bulk wet feed can not be taken away with the wind, easily makes the product heat damage.Wind speed is too high, and the systemic resistance increase is too big, and product moisture is also wayward.(2) air quantity gas-solid weight ratio is 5～10 o'clock, and moisture eliminator can normally move preferably.(3) time of drying is generally at 1～2s.(4) air themperature is 140～160 ℃.(5) blast is used for the various pressure-losseses of supplemental air flow drying tube.
Dry starch generally contains moisture 12～14%.Even in order to guarantee finish fineness, also to carry out the finished product arrangement sometimes.Handle through screening earlier, sift out the starch of regulation fineness, screen overflow is sent into pulverizer and is pulverized, and then sieves, and makes product all reach the fineness of regulation.
Preferable, as a kind of embodiment, the concentration of sulfurous acid is 0.15%～0.25% in described first soak solution, and soaking temperature is 45 ℃～50 ℃, and soak time is 40～60 hours.
Because the environment difference of various places, so the corn soaking condition of various places factory is incomplete same.General operational requirement(GOR) is as follows: the sulfur dioxide concentration that soaks water is that 0.15%～0.2%, pH value is 3.5.In immersion process, sulfurous gas is by Maize Absorption, and concentration reduces gradually, and it is 3.9～4.1 that the concentration of the interior sulfurous gas of final immersion water of discharging is about 0.01%～0.02%, pH value; Soaking water temp is 50～55 ℃; Soak time is 40～60h.Soaking conditions should determine according to the quality of corn.Normally storing corn of a specified duration and flint corn all needs stronger soaking conditions, the sulfur dioxide concentration of namely having relatively high expectations, temperature and long soak time.Corn is through after soaking, and containing moisture should reach more than 40%.
Preferable, as a kind of embodiment, in the step (2), containing zymin in the process water in the described technology water pot, described enzyme preparation amount is 0.005%～0.01% of corn gross weight, and the temperature of Jin Paoing is 30 ℃～55 ℃ again, and pH is 3.0～6.0.
Preferable, as a kind of embodiment, the 3rd soak solution that obtains in the step (2) is back to the technology water pot and recycles.
Preferable, as a kind of embodiment, in the step (4), comprise that also to filametntary carrying out washing treatment, the washings that carrying out washing treatment obtains is mixed to the 3rd mixture.
Preferable, as a kind of embodiment, described zymin is the combination of cellulase and zytase, and the content of wherein said cellulase is 20%～40% by weight, and the content of described zytase is 60%～80% by weight.
Preferable, as a kind of embodiment, the content of described cellulase is 30% ± 1% by weight, the content of described zytase is 70% ± 1% by weight.
Preferable, as a kind of embodiment, described cellulase is to be made by Li Shi Trichoderma kind, in PH3.8～4.2, under the condition that temperature is 50 ℃, the transformation period of described cellulase is 96～144 hours.
Preferable, as a kind of embodiment, described zytase is to be made by aspergillus niger strain, in PH3.8～4.2, under the condition that temperature is 50 ℃, the transformation period of described zytase is 96～144 hours.
Preferable, as a kind of embodiment, described carrying out washing treatment adopts all is the process waters that added in the technology water pot of zymin.
In order to make purpose of the present invention, technical scheme and advantage clearer, below in conjunction with embodiment, the method for Production by Enzymes W-Gum of the present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explaining the present invention, and be not used in restriction the present invention.
Embodiment one
1, production unit: the production line of the wet processes W-Gum of 1200 ton per days.
2, the interpolation of zymin: add zymin (cellulase 30%, zytase 70%), addition is 0.005%.Zymin is added point and is the technology water pot, and continuous flow adds 30 days.In the production process of wet processes W-Gum, the conveying of raw material relies on process water to carry, and except starch washs with the new water, other is as germ washing, and the fiber wash water is process water, and process water can be recycled.
3, starch recoveries sees Table one.
4, performance analysis:
The starch recovery rate improves 0.5%, and namely fecund is given birth to starch=1200 ton per days * 0.5%=6 ton per day.In 3200 yuan/ton of market minimum rates, direct economic benefit=6 ton per days * 3200 yuan/ton=19,200 yuan/day.
The protein recovery rate improves 0.3%, and namely gold produces protein=1200 ton per days * 0.3%=3.6 ton per day.In 5000 yuan/ton of market minimum rates, direct economic benefit=3.6 ton per days * 5000 yuan/ton=18,000 yuan/day.
Embodiment two
1, production unit: the production line of the wet processes W-Gum of 1200 ton per days.
2, the interpolation of zymin: add zymin (cellulase 30%, zytase 70%), addition is 0.01%.Zymin is added point and is the technology water pot, and continuous flow adds 30 days.In the production process of wet processes W-Gum, the conveying of raw material relies on process water to carry, and except starch washs with the new water, other is as germ washing, and the fiber wash water is process water, and process water can be recycled.
3, starch recoveries sees Table one.
4, performance analysis:
The starch recovery rate improves 0.9%, and namely fecund is given birth to starch=1200 ton per days * 0.9%=10.8 ton per day.In 3200 yuan/ton of market minimum rates, direct economic benefit=10.8 ton per days * 3200 yuan/ton=33,480 yuan/day.
The protein recovery rate improves 0.4%, and namely gold produces protein=1200 ton per days * 0.4%=4.8 ton per day.In 5000 yuan/ton of market minimum rates, direct economic benefit=4.8 ton per days * 5000 yuan/ton=24,000 yuan/day.
The product yield that table one adds before and after the zymin compares
| Classification (%) | Before enzyme-added | Enzyme-added back (enzyme concentration 0.005%) | Enzyme-added back (enzyme concentration 0.01%) |
| Starch recoveries | 69 | 69.5 | 69.9 |
| Protein yield | 4.8 | 5.1 | 5.2 |
| The fiber yield | 11 | 11.5 | 11.5 |
| The plumule yield | 10 | 10.2 | 10.4 |
| Fiber moisture | 62 | 58 | 56 |
| Plumule moisture | 60 | 57 | 55 |
| Albumen moisture | 58 | 56 | 55 |
The above embodiment has only expressed several embodiment of the present invention, and it describes comparatively concrete and detailed, but can not therefore be interpreted as the restriction to claim of the present invention.Should be pointed out that for the person of ordinary skill of the art without departing from the inventive concept of the premise, can also make some distortion and improvement, these all belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
Claims (10)
1. the method for a Production by Enzymes W-Gum is characterized in that: comprise the steps:
(1) corn just soaks: corn soaking in first soak solution, is discharged soak solution greater than 40% the time when moisture in the corn, obtain first soaking corn and second soak solution through first immersion treatment, become corn steep liquor after the described second soak solution evaporation concentration;
(2) corn soaks again: carry out immersion treatment again in the first soaking corn of the process water in the adopting process water pot to described discharge soak solution, the immersion treatment time is 5～7 hours again, sieves processing after immersion treatment; Obtain through soaking corn again and the 3rd soak solution of immersion treatment again;
(3) fragmentation and separated plumule: the soaking corn again that step (2) is obtained carries out obtaining first mixture and separated plumule after broken apart plumule is handled;
(4) correct grinding, separated fiber: first mixture that step (3) is obtained finish grindes to handle and obtains second mixture, next second mixture is carried out separating treatment and obtains the 3rd mixture and fiber yarn;
(5) centrifugation: the 3rd mixture that step (4) is obtained carries out centrifugation to be handled and obtains W-Gum mixture and protein mixture;
(6) washing and dehydration: the W-Gum mixture that step (5) is obtained washs with processed and obtains wet corn starch;
(7) drying: the wet corn starch that step (6) is obtained carries out the W-Gum that drying treatment obtains drying.
2. method according to claim 1, it is characterized in that: in the step (1), the concentration of sulfurous acid is 0.15%～0.25% in described first soak solution, and soaking temperature is 45 ℃～50 ℃, and soak time is 40～60 hours.
3. method according to claim 1, it is characterized in that: in the step (2), contain zymin in the process water in the described technology water pot, described enzyme preparation amount is 0.005%～0.01% of corn gross weight, the temperature of Jin Paoing is 30 ℃～55 ℃ again, and pH is 3.0～6.0.
4. method according to claim 1, it is characterized in that: the 3rd soak solution that obtains in the step (2) is back to the technology water pot and recycles.
5. method according to claim 1 is characterized in that: in the step (4), comprise that also to filametntary carrying out washing treatment, the washings that carrying out washing treatment obtains is mixed to the 3rd mixture.
6. method according to claim 3, it is characterized in that: described zymin is the combination of cellulase and zytase, and the content of wherein said cellulase is 20%～40% by weight, and the content of described zytase is 60%～80% by weight.
7. method according to claim 6, it is characterized in that: the content of described cellulase is 30% ± 1% by weight, the content of described zytase is 70% ± 1% by weight.
8. method according to claim 6, it is characterized in that: described cellulase is to be made by Li Shi Trichoderma kind, in pH3.8～4.2, under the condition that temperature is 50 ℃, the transformation period of described cellulase is 96～144 hours.
9. method according to claim 6, it is characterized in that: described zytase is to be made by aspergillus niger strain, in pH3.8～4.2, under the condition that temperature is 50 ℃, the transformation period of described zytase is 96～144 hours.
10. according to any described method of claim of claim 1～9, it is characterized in that: described carrying out washing treatment adopts all is the process waters that added in the technology water pot of zymin.
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| CN103665171A (en) * | 2013-11-26 | 2014-03-26 | 郑州市中食农产品加工研究院 | Method for steeping corn in production process of corn starch |
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| CN105801712A (en) * | 2016-05-19 | 2016-07-27 | 重庆市农业科学院 | Corn soaking method in waxy corn starch production |
| CN106366205A (en) * | 2016-08-30 | 2017-02-01 | 广西慧投互联网金融服务有限公司 | Preparation method of corn starch milk |
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| CN105801712A (en) * | 2016-05-19 | 2016-07-27 | 重庆市农业科学院 | Corn soaking method in waxy corn starch production |
| CN106366205A (en) * | 2016-08-30 | 2017-02-01 | 广西慧投互联网金融服务有限公司 | Preparation method of corn starch milk |
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| CN109234184B (en) * | 2018-06-24 | 2020-04-28 | 山东省科学院生物研究所 | Direct-throwing microbial agent and application thereof in wet corn soaking |
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