CN103250635B - Breeding method and seed production method of high-grain-weight and high-quality two-line hybrid rice - Google Patents

Breeding method and seed production method of high-grain-weight and high-quality two-line hybrid rice Download PDF

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CN103250635B
CN103250635B CN201310215397.1A CN201310215397A CN103250635B CN 103250635 B CN103250635 B CN 103250635B CN 201310215397 A CN201310215397 A CN 201310215397A CN 103250635 B CN103250635 B CN 103250635B
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hybrid rice
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CN103250635A (en
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姚立生
孙明法
严国红
王爱民
朱国永
唐红生
何冲霄
任仲玲
刘凯
张桂云
施伟
单忠德
孙红芹
万林生
倪正斌
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YANCHENG MINGTIAN SEED SCIENCE AND TECHNOLOGY Co Ltd
Jiangsu Institute of Coastal Area Agricultural Sciences
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YANCHENG MINGTIAN SEED SCIENCE AND TECHNOLOGY Co Ltd
Jiangsu Institute of Coastal Area Agricultural Sciences
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Abstract

The invention discloses a breeding method and a seed production method of high-grain-weight and high-quality two-line hybrid rice, belonging to the technical field of plant breeding. The breeding method comprises the steps of: selecting natural variants which are slightly large in increment, slightly high in plant, slightly large in ear type and slightly high in grain weight from a Guangzhan 63S growth population group, wherein the photo-thermo sensitive genic male sterile line characters of the natural variants are similar to those of the Guangzhan 63S; from the F2 generation, in contrast with the Guangzhan 63S for each generation, selecting excellent single plants which have the photo-thermo sensitive genic male sterile line characters, the quality and the disease resistance similar to those of the Guangzhan 63S, are slightly large in growth and slightly large in ear type until indica type photo-thermo sensitive genic male sterile line salts, 220 S which are compact in plant type, strong in disease resistance, high in combining ability and high in quality, are bred in the F6 generation, wherein the thousand seed weight of the excellent single plants is about 29g and the thousand seed weight of the indica type photo-thermo sensitive genic male sterile line salts 220 S is 29g; and then preparing a hybrid rice combination from the salts 220S and excellent restorers Yanhui 888 to obtain Yanliangyou 2208 as a new two-line hybrid indica rice variety, which is compact in plant type, moderate in harvesting period and high in grain weight, and resists rice blast and bacterial blight, wherein the quality of the Yanliangyou 2208 reaches the third-grade national standard.

Description

A kind of breeding method and producing method for seed of high-grain weight excellent-quality double-linear hybrid rice kind
One, technical field
The breeding method and the producing method for seed that the present invention relates to a kind of high-grain weight excellent-quality double-linear hybrid rice kind, belong to field of plant variety breeding technology, more specifically says a kind of breeding and producing method for seed of high-grain weight excellent-quality double-linear hybrid rice kind.
Two, background technology
Paddy rice is the staple food crop of China, and the cultivated area of hybrid rice accounts for the over half of China's Monitoring of Paddy Rice Plant Area.The improvement of double-linear hybrid rice kind is the major fields of China's agri-scientific research in the recent period, and high-quality, high yield, many anti-, wide fitting are the important long-term targets of rice breeding.It is one of important channel of realizing China's breeding of hybridized rice third phase target that the ideotype that Academician YUAN Long-ping proposes and hybrid vigour combine.
Nearly ten years, China's breeding of hybridized rice research obtains the achievement of attracting attention in the Liao Ling world, in succession being bred as the super double-linear hybrid rice such as " two line system ", " Liangyou0293 ", " excellent No. 2 of Y two " newly combines, and realizing China's super rice target of hundred mu of first, second and third phases of high yield side's per mu yield, mark China's rice breeding and production continue to keep world lead level.The breeding objective that China's super hybrid rice is realized first, second and third phase is also the yield level in hundred mu of sides and thousand mu of high yield sides, and the yield level that China paddy rice large area is produced has just been realized per mu yield and exceeded 500 kilograms, average yield per mu does not also reach 550 kilograms of levels.For many years, domestic and international many colleagues study and draw, increasing thousand kernel weight is one of important channel of further improving rice varieties yield per unit area.At present, inharmonic defect such as the super double-linear hybrid rice new varieties ubiquity high-quality of application, output heavy with high grain and high-quality in production.The high grain of seed selection weigh and reach national fine quality rice standard, the good new hybrid rice varieties of other bulk properties is further to improve China's hybrid rice production output and meet the how anti-effective way of high-quality.
Three, summary of the invention
Technical problem
The object of the invention is to solve above-mentioned problems of the prior art with not enough, applicant is through experimental study for many years, in many hybridization hybridization schemes and selection, find, breeding and the producing method for seed of the heavy excellent double-linear hybrid rice of a kind of high grain, can well realize the problem that goal of the invention exists to solve prior art, and successfully select the double-linear hybrid rice new varieties salt two excellent 2208 that high grain is heavy, rice quality reaches three grades of superior rice standards of GB.
Technical scheme
Breeding and the producing method for seed of a kind of high-grain weight excellent-quality double-linear hybrid rice kind provided by the invention, comprise the following steps:
A) in the indica type photo-thermo-sensitive genic male sterile line Guangzhan 63S plantation of high-quality, blast resisting, high-combining ability colony, obtain that amount of growth is bigger, plant is slightly high, fringe type is bigger, sterile strain 1 strain of grain natural variation heavily slightly high, that photo-thermo-sensitive genic male sterile line characteristic is similar to Guangzhan 63S, while cutting root and stem of certain plants regeneration maturation, collect this natural variation strain seed (F 1);
B) then selfing limit in limit is selected, from selfing 2 generation (F 2) start, every Dai Jun is taking indica type photo-thermo-sensitive genic male sterile line Guangzhan 63S as contrast, and selective light temperature-sensitive sterile line characteristic, quality, disease resistance are similar to Guangzhan 63S, and amount of growth is bigger, fringe type is bigger, the fine individual plant of 29 grams of left and right of thousand kernel weight, to selfing 6 generation (F 6) be bred as that plant type compactness, disease resistance are strong, the indica type photo-thermo-sensitive genic male sterile line salt 220S of 29 grams of high-combining abilities, high-quality, thousand kernel weight;
Be that the extensive 888 preparing hybrid rice of restorer salt combine with the indica type photo-thermo-sensitive genic male sterile line salt 220S and two of CGMCCNO.7558, obtain high-grain weight excellent-quality double-linear hybrid rice new varieties.
Be bred as high-grain weight excellent-quality double-linear hybrid rice new varieties, name as salt two excellently 2208, it is characterized in that:
Excellent 2208 seedling of salt two are short strong, the short and thick green of bud scale, and blade is straight and upright, and leaf look dark green, and stooling is stronger; Strain plant type is collecting and distributing moderate, the average 125cm of plant height, and the total blade 17-18 of stem opens, 5 extend internodes, three, top functional leaf is straight and upright, is subject to light attitude good, lifts and is straw mulch top, the blue or green seed yellow maturity lover of bar when ripe on sword-like leave; The percentage of earbearing tiller is average 65%, effectively fringe 15-17 ten thousand of general mu, and effectively fringe 15.8 ten thousand of average mu, fringe type is larger, the average 25.4cm of spike length, average 168.5 of every total grain panicle number, the real grain of every fringe is several more than 140, and ripening rate is more than 80%, average 30 grams of thousand kernel weight; In average 139.5 days of the time of infertility, than contrast II excellent 838 ripe 5 days late, belong to basic nutrition vegetative form, in ripe middle Xian two-line hybrid rice type.
The producing method for seed of described high-grain weight excellent-quality double-linear hybrid rice new varieties, is characterized in that:
The producing method for seed of salt two excellent 2208 is the extensive 888 point of two phase sowings of male parent salt, I phase male parent is sowed by the end of April, II phase male parent was compared with I phase male parent late sowing 10 days, maternal compared with I phase male parent late sowing 35-36 days, seed farm Parent line number ratio is 2:12, I, this alternate planting of II phase male parent list, maternal seeding row spacing 13cm × 16.5cm, every cave 2-3 originally, plants sufficient Basic Seedling.
Beneficial effect
Compared with prior art, the breeding of a kind of high-grain weight excellent-quality double-linear hybrid rice of the present invention kind and producing method for seed tool have the following advantages and good effect:
1, adopt the breeding method of a kind of high-grain weight excellent-quality ternary hybrid rice variety provided by the present invention, be bred as the indica type photo-thermo-sensitive genic male sterile line salt of high-grain weight excellent-quality 220S.
Salt 220S seedling is short strong, and blade is slightly wide, and blade is endured, leaf look light green, tillering ability is stronger, and property in great numbers is good, strain plant type is collecting and distributing moderate, plant height 85cm left and right, 6 internodes, the total blade 15-17 of stem leaf, stooling is stronger, single-strain tassel number 8-10, spike length 23.0cm left and right, the total grain husk flower of every fringe 150-160 grain, grain length 6.5mm, aspect ratio 2.8,29 grams of thousand kernel weight, rice quality leading indicator reaches the more than three grades superior rice standard of GB.Paddy look slightly dark, clever sharp bar yellow, and without tip awn, fringe top is degenerated slightly heavy.
Salt 220S is sterile, and the phase is longer, and steady sterile period is about 50 days, and sterile phase pollen abortion types is without pollen or a little abortive pollen type, and in the sterile phase, sterile plant rate reaches 100%, and pollen sterility degree is 100%, and self-fruitful rate is 0%.Sterility in the sterile phase and can to educate fertility restorer in the phase all better.2012, according to China Paddy Rice Inst's climatic cabinate Fertility identification: salt 220S sterility is stable, fertility conversion origin temp was lower, and under 14.5h long-day condition, critical male sterile temperature is lower than 24 DEG C; Under 11.5h short-day condition, critical male sterile temperature is lower than 24 DEG C.
Salt 220S in Yancheng Region, Jiangsu Province for sowing about the hybrid rice production of hybrid seeds general June 5, heading mid-August, general duration from seeding to heading 84-85 days, the time of infertility 115-117 days.Belong to Xian male sterile line type in precocity.Under the normal weather of early and middle ten days in August, the morning, 9:00 began to spend, and 10:30-11:30 enters full-bloom stage, opens grain husk and lasts 30~45 minutes to closing grain husk, and single tassel blossom is gone through 4~5 days phases, the anthesis duration 8-9 days of colony.Column cap is colourless, stigma exposing ratio 78.3%, and wherein bilateral exposing ratio 43.5%, outcrossing seed-setting rate 46.6% left and right, responsive to " 920 ", outcrossing is good, general mu 200 kilograms of left and right of hybrid seed yield, high yield field mu hybrid seed yield is more than 300 kilograms.
2, adopt breeding and the producing method for seed of a kind of high-grain weight excellent-quality double-linear hybrid rice kind provided by the present invention, be bred as high-grain weight excellent-quality double-linear hybrid rice new varieties salt two excellent 2208.
A) stable high yield, yield potentiality is large
2010-2011, salt two excellent 2208 is participated in China's medium rice district, middle and lower reach of Yangtze River multiple spot variety comparative test, and two 579.41 kilograms of annual per mu yields, than excellent 838 volume increase 4.73% of contrast II, than the excellent 838 volume increase point ratios 77.9% of contrast II; Than rich two excellent No. 4 volume increase 3.84% of contrast, than the rich two excellent No. 4 volume increase point ratios 73.3% of contrast, all reach utmost point significance level.
Within 2012, participate in China medium rice district, middle and lower reach of Yangtze River multiple spot great Qu variety comparative test, 571.39 kilograms of average yield per mus, than excellent 838 volume increase 8.15% of contrast II, than the excellent 838 volume increase point ratios 100% of contrast II.
B) best in quality, commodity is good
Excellent 2208 grain of salt two are elongated, slightly short awns, and it is yellow that grain is, and plumpness is good, and grain of rice transparency is good.Within 2011, detect through Ministry of Agriculture's rice and quality of item supervision and inspection center: brown rice rate 81.3%, polished rice rate 73.4%, head rice rate 65.4%, grain length 7.1mm, aspect ratio 3.0, the white rate 28.0% of chalk, chalkiness degree 3.5%, 1 grade of transparency, disappear 7.0 grades of values of alkali, glue denseness 79mm, amylose content 17.4%, all indexs of rice quality all reach three grades of GBs, standard laid down by the ministries or commissions of the Central Government secondary superior rice standard.
C) disease resistance is outstanding, and yield stability is good
2010-2011 carries out Disease Resistance Identification for continuous 2 years, the excellent 2208 pairs of rice blast of salt two, bacterial leaf spot resistance are obviously better than check variety II excellent 838, excellent 838 similar to Brown Planthopper Resistance and check variety II, its specific targets are: 5.7 grades of rice blast composite indexes (CK7.2 level), highest 9 grades (the CK9 level) of fringe pest percent of loss; 5 grades of bacterial leaf-blight average levels (CK7 level), highest 5 grades (CK7 level); 8 grades of brown planthopper average levels (CK9 level), highest 9 grades (CK9 level).Rice blast, bacterial leaf-blight and Brown Planthopper Resistance qualification are with reference to national new rice variety pilot experiment standard: 0 grade (high resistance), 1 grade (resist), 3 grades (in anti-), 5 grades (middle sense), 7 grades (sense), 9 grades (height is felt).Because excellent 2208 canes of salt two are sturdy, leaf sheath is embraced stem consolidation, has good fertilizer resistance anti-lodging performance.
D) economical character is good, wide adaptability
Excellent 2208 seedling of salt two are short strong, the short and thick green of bud scale, and blade is straight and upright, and leaf look dark green, and stooling is stronger.Strain plant type is collecting and distributing moderate, plant height 125cm left and right, and the total blade 17-18 of stem opens, 5 extend internodes, three, top functional leaf is straight and upright, is subject to light attitude good, lifts and is straw mulch top, the blue or green seed yellow maturity lover of bar when ripe on sword-like leave.The percentage of earbearing tiller 65% left and right, effectively fringe 15-17 ten thousand of general mu, effectively fringe 15.8 ten thousand of average mu, fringe type is larger, spike length 25.4cm, 168.5 left and right of every total grain panicle number, the real grain of every fringe is several more than 140, and ripening rate is more than 80%, 30 grams of thousand kernel weight.In the 139.5 days time of infertility, than contrast II excellent 838 ripe 5 days late, belong to basic nutrition vegetative form, in ripe middle Xian double-linear hybrid rice type.
Four, brief description of the drawings
Accompanying drawing 1: be the excellent 2208 breeding pedigree schematic diagrames of high-grain weight excellent-quality double-linear hybrid rice Variety Salt two of the present invention
Biological preservation
Salt 220S, Classification And Nomenclature is paddy rice (Oryzasativa), be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on May 6th, 2013, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, address institute of microbiology of the Chinese Academy of Sciences, preserving number is: CGMCCNo.7558.
Five, embodiment
Further illustrate a kind of breeding and producing method for seed of high-grain weight excellent-quality double-linear hybrid rice kind below in conjunction with accompanying drawing 1.
1, breeding method
A) in the indica type photo-thermo-sensitive genic male sterile line Guangzhan 63S plantation of high-quality, blast resisting, high-combining ability colony, obtain that amount of growth is bigger, plant is slightly high, fringe type is bigger, sterile strain 1 strain of grain natural variation heavily slightly high, that photo-thermo-sensitive genic male sterile line characteristic is similar to Guangzhan 63S, while cutting root and stem of certain plants regeneration maturation, collect this natural variation strain seed (F 1);
B) then selfing limit in limit is selected, from selfing 2 generation (F 2) start, every Dai Jun is taking indica type photo-thermo-sensitive genic male sterile line Guangzhan 63S as contrast, and selective light temperature-sensitive sterile line characteristic, quality, disease resistance are similar to Guangzhan 63S, and amount of growth is bigger, fringe type is bigger, the fine individual plant of 29 grams of left and right of thousand kernel weight, to selfing 6 generation (F 6) be bred as that plant type compactness, disease resistance are strong, the indica type photo-thermo-sensitive genic male sterile line salt 220S of 29 grams of high-combining abilities, high-quality, thousand kernel weight;
What C) be bred as with the above indica type photo-thermo-sensitive genic male sterile line salt 220S being bred as and Jiangsu Inst. of Coastal Area Agricultural Sciences has plant type compactness, high-quality, extensive 888 preparing hybrid rice combinations of superior restorer line salt disease-resistant, high yield again, obtains plant type compactness, suitable middle and high grain of ripe phase is heavy, meter matter reaches three grades of superior rices of GB, middle blast resisting and bacterial leaf-blight two-line hybrid rice new varieties salt two excellent 2208.
Concrete breeding process is in table 1.
Table 1: the excellent 2208 breeding processes of high-grain weight excellent-quality double-linear hybrid rice Variety Salt two of the present invention
Adopt breeding and the producing method for seed of a kind of high-grain weight excellent-quality double-linear hybrid rice kind provided by the present invention, be bred as high-grain weight excellent-quality double-linear hybrid rice new varieties salt two excellent 2208.
A) stable high yield, yield potentiality is large
2010-2011, salt two excellent 2208 is participated in China's medium rice district, middle and lower reach of Yangtze River multiple spot variety comparative test, and two 579.41 kilograms of annual per mu yields, than excellent 838 volume increase 4.73% of contrast II, than the excellent 838 volume increase point ratios 77.9% of contrast II; Than rich two excellent No. 4 volume increase 3.84% of contrast, than the rich two excellent No. 4 volume increase point ratios 73.3% of contrast, all reach utmost point significance level.
Within 2012, participate in China medium rice district, middle and lower reach of Yangtze River multiple spot great Qu variety comparative test, 571.39 kilograms of average yield per mus, than excellent 838 volume increase 8.15% of contrast II, than the excellent 838 volume increase point ratios 100% of contrast II.
B) best in quality, commodity is good
Excellent 2208 grain of salt two are elongated, slightly short awns, and it is yellow that grain is, and plumpness is good, and grain of rice transparency is good.Within 2011, detect through Ministry of Agriculture's rice and quality of item supervision and inspection center: brown rice rate 81.3%, polished rice rate 73.4%, head rice rate 65.4%, grain length 7.1mm, aspect ratio 3.0, the white rate 28.0% of chalk, chalkiness degree 3.5%, 1 grade of transparency, disappear 7.0 grades of values of alkali, glue denseness 79mm, amylose content 17.4%, all indexs of rice quality all reach three grades of GBs, standard laid down by the ministries or commissions of the Central Government secondary superior rice standard.
C) disease resistance is outstanding, and yield stability is good
2010-2011 carries out Disease Resistance Identification for continuous 2 years, the excellent 2208 pairs of rice blast of salt two, bacterial leaf spot resistance are obviously better than check variety II excellent 838, excellent 838 similar to Brown Planthopper Resistance and check variety II, its specific targets are: 5.7 grades of rice blast composite indexes (CK7.2 level), highest 9 grades (the CK9 level) of fringe pest percent of loss; 5 grades of bacterial leaf-blight average levels (CK7 level), highest 5 grades (CK7 level); 8 grades of brown planthopper average levels (CK9 level), highest 9 grades (CK9 level).Rice blast, bacterial leaf-blight and Brown Planthopper Resistance qualification are with reference to national new rice variety pilot experiment standard: 0 grade (high resistance), 1 grade (resist), 3 grades (in anti-), 5 grades (middle sense), 7 grades (sense), 9 grades (height is felt).Because excellent 2208 canes of salt two are sturdy, leaf sheath is embraced stem consolidation, has good fertilizer resistance anti-lodging performance.
D) economical character is good, wide adaptability
Excellent 2208 seedling of salt two are short strong, the short and thick green of bud scale, and blade is straight and upright, and leaf look dark green, and stooling is stronger.Strain plant type is collecting and distributing moderate, plant height 125cm left and right, and the total blade 17-18 of stem opens, 5 extend internodes, three, top functional leaf is straight and upright, is subject to light attitude good, lifts and is straw mulch top, the blue or green seed yellow maturity lover of bar when ripe on sword-like leave.The percentage of earbearing tiller 65% left and right, effectively fringe 15-17 ten thousand of general mu, effectively fringe 15.8 ten thousand of average mu, fringe type is larger, spike length 25.4cm, 168.5 left and right of every total grain panicle number, the real grain of every fringe is several more than 140, and ripening rate is more than 80%, 30 grams of thousand kernel weight.In the 139.5 days time of infertility, than contrast II excellent 838 ripe 5 days late, belong to basic nutrition vegetative form, in ripe middle Xian double-linear hybrid rice type.
2, the excellent 2208 culture technique main points of salt two
According to testing and demonstration result through multiple years, the structure of output that the excellent 2208 establishing in large scale per mu yield of salt two is 650 kilograms is: mu is fringe 16-18 ten thousand effectively, every total grain panicle 175 number left and right, ripening rate 90% left and right, 30 grams of left and right of thousand kernel weight.High-yield culturing should striven sufficient fringe, attack on the basis of large fringe, improves ripening rate.By cultivating the strong seedling of of the right age many tillers, rational close planting, shortly early sends out early enough seedling and puts in time field, improves colony quality, accomplishes fluently high yield basis, on the basis of guaranteeing suitable spike number, and the short strong large fringe of stalk, thus reach sufficient fringe, large fringe, high grain and take back again the object of high yield.The high-yield culture technique main points that should grasp are:
(1) proper seeding, cultivates strong seedling
According to various places Rice Production custom, reasonable arrangement sowing time, guarantee safety heading flowering.The every mu of clean seedling plate amount of broadcasting 10-15 kilogram of water-raised seedling, the every mu of clean seedling plate amount of broadcasting 20-25 kilogram of non-irrigated seedling, land for growing field crops sowing quantity 1.0-1.5 kg/acre.Transplanting 20-25 day in general May is sowed, every mu of land for growing field crops sowing quantity 1.5-2.0 kilogram.Rice seedling bed will use sufficient base manure, early executes wean fertile, imposes long thick short fetilizer for tillering, send and transfer fertilizer, and carry out rice seedling bed disease pest and weed control, cultivates the strong seedling of of the right age many tillers.
(2) transplant rational close planting in good time
Transplant, rational close planting is the basis of high-yield culturing in good time.Water-raised seedling length of time rice seedlings grow 30-35 days, the non-irrigated seedling length of time rice seedlings grow was advisable with 20-25 days, general 18-20 days of the transplanting length of time rice seedlings grow.In upper fertility field plant soon specification 1.6-1.8 ten thousand caves/mu left and right, every cave 1-2 this, plant sufficient Basic Seedling 6-8 ten thousand/mu, it is 1.8-2.0 ten thousand cave/mus that the field that fertility is low will suitably increase Planting Densities, accomplishes shallow slotting, even cultivation while planting, guarantees to plant quality.
(3) scientific utilization of fertilizer, short seedling is early sent out
Scientific utilization of fertilizer, it is the key that forms yielding Populations that short seedling is early sent out.Excellent 2208 per mu yields of salt two more than 650 kilograms are cultivated, general fertilizer requirement amounts to purity nitrogen 20 kgs/acre of left and right, phosphorus, potash fertilizer are used in collocation, phosphorus, potash fertilizer are main as base manure, fertilising should be adhered to " front short, in steady, rear benefit ", principle, base manure accounts for 60% left and right, tillering fertilizer accounts for 15% left and right, and fringe granulated fertilizer accounts for 25% left and right.
(4) run water slurry well, coordinate colony
Water starch management should take that shallow water transplants seedlings, very little water is lived, thin moisture tiller, deep water heading flowering, the irrigation method of later stage alternation of wetting and drying.Require to plant latter about 20 days, send out foot and wait fringe seedling, field is put in draining in good time, controls peak seedling and is no more than 250,000/mu, finally becomes fringe 16-17 ten thousand/mu, and moistening endowment of later stage, gathers in and cut off the water supply for first 5 days, guarantees the blue or green seed Huang of stalk.
(5) the anti-worm of curing the disease, the increasing fringe of keeping a full stand of seedings
Broadcast front with medicament seed soaking and prevent and treat the seed-borne disease such as bakanae disease and Aplelenchiodes bessegi of rice.Seeding stage attention control small brown rice planthopper, rice thrips, aphid, field period will be carried out the preventing and controlling of the damage by disease and insect such as control rice blast, bacterial leaf-blight, banded sclerotial blight, black streak dwarf, snout moth's larva, brown plant-hopper, ensures that rice seedling normal growth grows, and keeps a full stand of seedings to strive fringe and strive for high yield.
3, the excellent 2208 seeding technique main points of salt two
(1) reasonable arrangement is broadcast the poor phase, guarantees that flower synchronization is good
Salt two excellent 2208 is in the Yancheng, Jiangsu Province production of hybrid seeds, and first phase male parent is sowed by the end of April, and the second stage of male parent was compared with first phase male parent late sowing 10 days.Maternal early June sowing, broadcasts poor time difference 35-36 days with first phase male parent, the poor 6.5-7.0 leaf of leaf, and the such time difference and leaf are poor, and the general florescence can meet well.
(2) cultivate strong seedling, suitable row ratio, participates Basic Seedling
When General Requirements is transplanted, 3-4, male parent band is tillered, and 1-2, maternal band is tillered.Production of hybrid seeds old farmer, maternal row are than being advisable with 2:12, and plant at the two phase male parent duplicate rows intervals same period, distance between rows and hills 30 ㎝ × 15 ㎝; Maternal distance between rows and hills 16.7 ㎝ × 13.3 ㎝, stop action row, and every cave 2-3 originally, plants sufficient Basic Seedling.
(3) fertile water transfer control, puts up seedling frame, coordinates colony
Seed farm rich water is planned strategies for, and grasps the principle of " front heavy, in steady, rear benefit ", and base manure is wanted foot, and tillering fertilizer is wanted early, promotes Parent early growth and quick tiller, balanced growth.After enough seedlings, put in time field, control ineffective tillering; Late growth stage Dry-wet alternate irrigation, supports Gen Baoye, promotes Grain Filling.
(4) spray in right amount " 920 ", improve outcrossing seed-setting rate
Maternal salt 220S fringe type is large, bag neck degree is lighter, seed farm " 920 " consumption is too much unsuitable, general consumption 30-40 gram/acre, point three uses, consumption 10-15 gram/acre for the first time, see that at seed farm female parent fringe 5% starts to cut leaf and used the same day, for the second time, consumption is respectively 15-20 gram/acre and 5-10 gram/acre for the third time, for the first time " 920 " with after within second day and the 4th day, spraying, can solve preferably male sterile line bag neck problem, promote that fringe layer is neat, improve outcrossing seed-setting rate and production of hybrid seeds per mu yield.
(5) grasp the key link period, conscientiously carry out anti-assorted pure keeping work
Under the prerequisite of strict time and space isolation, florescence impurity elimination work is extremely important, is the most important thing of anti-assorted pure keeping work.Seed farm is from being shown in that fringe is to cutting leaf before " 920 " used and tell on, and approximately 6-7 days time, is the material time of florescence impurity elimination work, at the sturdy impurity elimination 3-4 time of this material time, can play a multiplier effect to improving seed production purity.In addition, during ripe and harvested, need guarantee that male parent and maternal single receipts, list shine, single storage, this is also to prevent one of the work of pure keeping of mixing.
(6) note diseases and pests controlling, improve seed commodity
During paddy growth, note the control of the damage by disease and insect such as rice thrips, aphid, banded sclerotial blight, snout moth's larva, rice fulgorid, to ensure that rice growth is normal.Note using anti-microbial type agricultural chemicals, to controlling disease, improve paddy look, improving seed commodity has remarkable result, and major measure has three, and the one, use jinggangmeisu, use twice booting stage, occur suppressing banded sclerotial blight, the strong stalk of healthy tree has obvious effect; The 2nd, use " going out black No. 1 " bactericide, female parent uses twice after cutting leaf, both can prevent and treat grain smut, can improve again paddy look; The 3rd, in good time harvesting, accomplishes " ninety percent ripe hundred per cent is received " and dries in time, is conducive to improve the commodity of germination rate and seed.
4, salt 220S propagation technique main points
Indica type photo-thermo-sensitive genic male sterile line salt 220S breeding: place is chosen in Sanya, Hainan, is arranged in annual mid or late November sowing time, Second Year heading mid-February, for best, easily obtains comparatively desirable reproductive output.

Claims (2)

1. a breeding method for high-grain weight excellent-quality double-linear hybrid rice kind, comprises the following steps:
With the indica type photo-thermo-sensitive genic male sterile line salt 220S and two that preserving number is CGMCC NO. 7558 be the extensive 888 preparing hybrid rice combinations of restorer salt, obtain high-grain weight excellent-quality double-linear hybrid rice new varieties, name as salt two excellently 2208, it is characterized in that:
Excellent 2208 seedling of salt two are short strong, the short and thick green of bud scale, and blade is straight and upright, and leaf look dark green, and stooling is stronger; Strain plant type is collecting and distributing moderate, the average 125cm of plant height, and the total blade 17-18 of stem opens, 5 extend internodes, three, top functional leaf is straight and upright, is subject to light attitude good, lifts and is straw mulch top, the blue or green seed yellow maturity lover of bar when ripe on sword-like leave; The percentage of earbearing tiller is average 65%, effectively fringe 15-17 ten thousand of general mu, and effectively fringe 15.8 ten thousand of average mu, fringe type is larger, the average 25.4cm of spike length, average 168.5 of every total grain panicle number, the real grain of every fringe is several more than 140, and ripening rate is more than 80%, average 30 grams of thousand kernel weight; In average 139.5 days of the time of infertility, than contrast II excellent 838 ripe 5 days late, belong to basic nutrition vegetative form, in ripe middle Xian double-linear hybrid rice type.
2. the producing method for seed of high-grain weight excellent-quality double-linear hybrid rice kind described in claim 1, is characterized in that:
The producing method for seed of salt two excellent 2208 is the extensive 888 point of two phase sowings of male parent salt, I phase male parent is sowed by the end of April, II phase male parent was compared with I phase male parent late sowing 10 days, maternal compared with I phase male parent late sowing 35-36 days, seed farm Parent line number ratio is 2:12, I, this alternate planting of II phase male parent list, maternal seeding row spacing 13cm × 16.5cm, every cave 2-3 originally, plants sufficient Basic Seedling.
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