CN103461097A - Combined seed production method for three-line hybrid oilseed rape - Google Patents

Combined seed production method for three-line hybrid oilseed rape Download PDF

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CN103461097A
CN103461097A CN 201310363314 CN201310363314A CN103461097A CN 103461097 A CN103461097 A CN 103461097A CN 201310363314 CN201310363314 CN 201310363314 CN 201310363314 A CN201310363314 A CN 201310363314A CN 103461097 A CN103461097 A CN 103461097A
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hybrid
rape
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sterile line
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岳绪国
顾炳朝
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JIANGSU FENGYUAN SEED Co Ltd
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JIANGSU FENGYUAN SEED Co Ltd
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Abstract

The invention discloses a combined seed production method for three-line hybrid oilseed rape, which comprises the following steps: selecting a cabbage type rape male sterile line as the female parent, selecting a cabbage type hybrid rape maintainer line ZhenB152 as the male parent, and performing hybrid transformation for multiple years to obtain a cabbage type rape male sterile line ZhenA152; selecting a cabbage type rape male sterile line as the female parent, selecting cabbage type rape as the male parent, and performing hybrid transformation for multiple years to obtain a cabbage type rape restorer line R023; mixed planting the female parent and the male parent in different lines, and harvesting cabbage type hybrid rape seeds in the mature period; hybridizing the stable maintainer line ZhenB152 and the sterile line ZhenA152 to breed a sterile line, and hybridizing the strong restorer line R023 and the sterile line ZhenA152 to produce hybrid seeds. The seeds prepared through the combined seed production method have high self regulation capability and are easy to plant and popularize, the individual is upright during the seedling stage, and grows healthily during the bolting period; the spring sprouting property is strong, the flower season is concentrated, the grains are dense, the yield components are coordinated, and the mature consistency is good. In addition, the seed production process of the seed production method is safe, convenient and simple.

Description

A kind of three-tine hybrid rape combination producing method for seed
Technical field
The invention belongs to the rapeseed breeding technical field, relate in particular to a kind of three-tine hybrid rape combination producing method for seed.
Background technology
Rape is China paddy rice of continuing, wheat, corn, the fifth-largest dominant crop after soybean, cultivated area and output all account for 30% left and right in the world, the sowing area in rape district, the Yangtze river basin, output all accounts for more than 85% of the whole nation, it is the most concentrated double-low rapeseed advantage producing region of China, along with improving constantly of rapid development of economy and living standards of the people, market is increasing to the demand of rape seed, and industry occupies cultivated land, area sharply increases, the rapeseed cultivation area reduces relatively, imbalance between supply and demand is more outstanding, international and domestic supply is tight slightly, the whole world is to the vegetable oil increase in demand, at present, it is nervous that world's grease supply has been tending towards, China faces the problem of oil supply quantity not sufficient equally, according to expert research and analysis, 2005-2010, whole world vegetable oil consumption will increase 27%, edible consumption increases 14%, biofuel increases 13%, the yield per unit area that how to improve rape seed just seems particularly important, Jiangsu is located in the middle and lower reach of Yangtze River, the rapeseed cultivation area, total producing, per unit area yield lays respectively at the 5th of the whole nation, the 3rd, the 1st, there is very important status, due to applying of cross-bred rape, the Jiangsu yield of rape improves constantly, bring up to 2550kg/hm2 in 2009 from the 2130kg/hm2 of 2003, increased production 19.7%, unit yield is positioned at first of the whole nation, higher more than 1/3 than the average per unit area yield of national rape, according to statistics, 2011, the Qin's serial cross-bred rape kind cultivated area of oil accounts for nearly 7 one-tenth of the whole province's rapeseed cultivation area, it is the leading kind steadily improved of yield of rape, therefore, study and improve cross-bred rape matched breeding and culture technique, finally to bring into play its Yield Competition advantage, the important channel of performance high yield characteristic, breeding man is strengthening the rape heterosis utilization, be measured a large amount of combinations every year to screen strong advantage combination, annual many combinations all will be carried out small size breeding processed to supply with the multiple spot compatibility test, pilot experiment, attribute test, the needs that demonstration is planted experimentally, to accelerate propelling, utilize the existing potentiality of Super-high-yielding preferably, possessing again the two low strong advantage high-yield hybrid rapes of high-quality newly combines, promote the rape industrial competitiveness.
Therefore, the technical barrier that safety, small size three-tine hybrid rape combination producing method for seed rapeseed breeding man convenient, efficient, high yield run in concrete hybrid combination screening.
Summary of the invention
The purpose of the embodiment of the present invention is to provide a kind of three-tine hybrid rape combination producing method for seed, is intended to solve the problem of the rapeseed breeding man of three-tine hybrid rape combination production of hybrid seeds existence in concrete hybrid combination screening.
The embodiment of the present invention is achieved in that a kind of three-tine hybrid rape combination producing method for seed, and this three-tine hybrid rape combination producing method for seed comprises the following steps:
Select Brassica napus male sterile line as female parent, cabbage type rape hybrid maintainer town B152 makes male parent, by hybridizing for many years transformation, obtains Brassica napus male sterile line town A152;
It is maternal selecting Brassica napus male sterile line, with cabbage type rape, makes male parent, by hybridizing for many years transformation, obtains cabbage type rape restorer R023; The mixed planting of Parent branch, maturing stage results cabbage type rape hybrid seed;
Maintainer town B152 is stablized in utilization and sterile line propagation is carried out in A152 hybridization in male sterile line town, utilizes strong restorer R023 and male sterile line town A152 to carry out the hybrid seed production of hybrid seeds.
Further, in this three-tine hybrid rape combination producing method for seed, production of hybrid seeds type of seeding is: every community production of hybrid seeds area 20m 2, surrounding plantation male parent, Parent is planted altogether in the middle of 6 Hang, communities and stayed 1 spare row, Parent plantation is sequentially 1-2-0-2-1, line-spacing 35cm, male parent spacing in the rows 20cm, maternal spacing in the rows 15cm.
Further, in this three-tine hybrid rape combination producing method for seed, producing method for seed is: the isolation yarn account that long 6.7m for production of hybrid seeds community * wide 3.0m * high 3.0m nonwoven is made covers, the potted plant male parent of spare row, pluck the top processing to maternal employing of male sterile line, promotes that secondary divides branch growth and quantity.
Three-tine hybrid rape combination producing method for seed provided by the invention, the isolation yarn account of making by long 6.7m for production of hybrid seeds community * wide 3.0m * high 3.0m nonwoven covers, guarantee seed production purity, the potted plant male parent of spare row, be convenient to rich water regulate breeding time especially the florescence to reach the kind of blooming period prolonging Encounter Time, to regulate potted plant height to improve Pollination Effect, improve ripening rate, the maternal employing of male sterile line plucked top and processed simultaneously, promote that secondary divides branch growth and quantity, improved hybrid seed yield.Quality of the present invention is two low, meets national double-low rapeseed standard, and stalk break is lighter, the incidence of disease 5.83%, and disease refers to 2.96, cold resistance is good; Resistance is outstanding, and high and stable yield is good, and Self-regulation ability is strong, and easily cultivation is promoted, and individual seedling stage is upright, tongue phase robust growth, and the spring is sent out by force, and the florescence concentrates, and grain closeer, and structure of output is coordinated, and ripe uniformity is better.In addition, production of hybrid seeds process safety facility of the present invention, process is easy, uses that material is simple and easy to be easy to get, and processing ease is grasped, and economizes native abridged edition; The production of hybrid seeds is efficient, high yield, has promoted the rape industrial competitiveness.
The accompanying drawing explanation
Fig. 1 is the flow chart of the three-tine hybrid rape combination producing method for seed that provides of the embodiment of the present invention.
Embodiment
In order to make purpose of the present invention, technical scheme and advantage clearer, below in conjunction with embodiment, the present invention is further elaborated.Should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not intended to limit the present invention.
Fig. 1 shows three-tine hybrid rape combination producing method for seed flow process provided by the invention.For convenience of explanation, only show part related to the present invention.
The three-tine hybrid rape combination producing method for seed of the embodiment of the present invention, this three-tine hybrid rape combination producing method for seed comprises the following steps:
Select Brassica napus male sterile line as female parent, cabbage type rape hybrid maintainer town B152 makes male parent, by hybridizing for many years transformation, obtains Brassica napus male sterile line town A152;
It is maternal selecting Brassica napus male sterile line, with cabbage type rape, makes male parent, by hybridizing for many years transformation, obtains cabbage type rape restorer R023; The mixed planting of Parent branch, maturing stage results cabbage type rape hybrid seed;
Maintainer town B152 is stablized in utilization and sterile line propagation is carried out in A152 hybridization in male sterile line town, utilizes strong restorer R023 and male sterile line town A152 to carry out the hybrid seed production of hybrid seeds.
As a prioritization scheme of the embodiment of the present invention, in this three-tine hybrid rape combination producing method for seed, production of hybrid seeds type of seeding is: every community production of hybrid seeds area 20m 2, surrounding plantation male parent, Parent is planted altogether in the middle of 6 Hang, communities and stayed 1 spare row, Parent plantation is sequentially 1-2-0-2-1, line-spacing 35cm, male parent spacing in the rows 20cm, maternal spacing in the rows 15cm.
A prioritization scheme as the embodiment of the present invention, in this three-tine hybrid rape combination producing method for seed, producing method for seed is: the isolation yarn account that long 6.7m for production of hybrid seeds community * wide 3.0m * high 3.0m nonwoven is made covers, the potted plant male parent of spare row, the maternal employing of male sterile line plucked to the top processing, promote that secondary divides branch growth and quantity.
Below in conjunction with drawings and the specific embodiments, application principle of the present invention is further described.
As shown in Figure 1, the three-tine hybrid rape of embodiment of the present invention combination producing method for seed comprises the following steps soon:
S101:: select Brassica napus male sterile line as female parent, cabbage type rape hybrid maintainer town B152 makes male parent, by hybridizing for many years transformation, obtains Brassica napus male sterile line town A152;
S102: it is maternal selecting Brassica napus male sterile line, with cabbage type rape, makes male parent, by hybridizing for many years transformation, obtains cabbage type rape restorer R023; The mixed planting of Parent branch, maturing stage results cabbage type rape hybrid seed;
S103: maintainer town B152 is stablized in utilization and sterile line propagation is carried out in A152 hybridization in male sterile line town, utilizes strong restorer R023 and male sterile line town A152 to carry out the hybrid seed production of hybrid seeds;
Concrete steps of the present invention are:
The first step, select Brassica napus male sterile line as female parent, and cabbage type rape hybrid maintainer town B152 makes male parent, by hybridizing for many years transformation, obtains Brassica napus male sterile line town A152;
Second step, it is maternal selecting Brassica napus male sterile line, with cabbage type rape, makes male parent, by hybridizing for many years transformation, obtains cabbage type rape restorer R023; The mixed planting of Parent branch, maturing stage results cabbage type rape hybrid seed;
The 3rd step, maintainer town B152 is stablized in utilization and sterile line propagation is carried out in A152 hybridization in male sterile line town, utilizes strong restorer R023 and male sterile line town A152 to carry out the hybrid seed production of hybrid seeds;
The 4th step, production of hybrid seeds seeding technique improves: every community production of hybrid seeds area 20m 2, surrounding plantation male parent, Parent is planted altogether in the middle of 6 Hang, communities and stayed 1 spare row (as follows), Parent plantation be sequentially 1-2-0-2-1, line-spacing 35cm left and right, male parent spacing in the rows 20cm left and right, about maternal spacing in the rows 15cm;
The 5th step, the seeding technique method improvement: the isolation yarn account that long 6.7m for production of hybrid seeds community * wide 3.0m * high 3.0m nonwoven is made covers, guarantee seed production purity, the potted plant male parent of spare row, be convenient to rich water regulate breeding time especially the florescence to reach the kind of blooming period prolonging Encounter Time, to regulate potted plant height to improve Pollination Effect, improve ripening rate, the maternal employing of male sterile line plucked top and processed simultaneously, promote that secondary divides branch growth and quantity, improve hybrid seed yield.
In cultivation method of the present invention, select Brassica napus male sterile line as female parent, the cabbage type rape hybrid maintainer is made male parent, by cross breeding method transformation Brassica napus male sterile line, it is maternal selecting Brassica napus male sterile line, makes male parent with cabbage type rape, by cross breeding method transformation cabbage type rape restorer, the mixed planting of Parent branch, maturing stage results cabbage type rape hybrid seed, maintainer is stablized in utilization and sterile line propagation is carried out in male sterile line hybridization, utilizes strong restorer and male sterile line to carry out the hybrid seed production of hybrid seeds, and production of hybrid seeds seeding technique improves: every community production of hybrid seeds area 20m 2, surrounding plantation male parent, Parent is planted 6 row altogether, stay 1 spare row (as follows) in the middle of community, the Parent plantation is sequentially 1-2-0-2-1, line-spacing 35cm left and right, male parent spacing in the rows 20cm left and right, maternal spacing in the rows 15cm left and right, the seeding technique method improvement: the isolation yarn account that long 6.7m for production of hybrid seeds community * wide 3.0m * high 3.0m nonwoven is made covers, guarantee seed production purity, the potted plant male parent of spare row, be convenient to rich water regulate breeding time especially the florescence to reach the kind of blooming period prolonging Encounter Time, regulate potted plant height to improve Pollination Effect, improve ripening rate, the maternal employing of male sterile line plucked to the top processing simultaneously, promote that secondary divides branch growth and quantity, improve hybrid seed yield.
The specific embodiment of the present invention:
Embodiment
1) spring in 2000 is maternal with two low strain 99S18, and recovering strain R068 is male parent, the preparing hybrid combination;
2) 2002 from F2 for starting the two low proterties of seed selection and recovering proterties, selfing screen to 2009 NianF9Yong town A152 test crosses, the new combination of 2009-2010 identifies and surveys product, combination number is SR18 then, restorer is numbered R023;
3) autumn sowing in 2011 is recommended to participate in the Jiangsu cross-bred rape and is newly combined prerun;
4) on September 20th, 2011 according to embodiment according to Parent is sowed according to 1-2-0-2-1;
5) carrying out the sowing of basin cylinder male parent on September 27th, 2011;
6) on March 18th, 2012 the male sterile line female parent being plucked to top processes;
7) placement basin cylinder male parent on March 19th, 2012 (6,7 steps can complete in a day);
8) lodge isolation on March 20th, 2012 mosquito net;
9) adjusting basin cylinder height on March 21st, 2012 (8,9 steps can complete in a day);
10) about 20 days in April, 20012, remove the isolation mosquito net and remove except basin cylinder male parent, cut off male parent;
11) harvesting production of hybrid seeds (male sterile line or crossbreed), threshing, raise clean, the Sai that dries in the air, storage in by the end of May, 20012,
Specifically implement process in Table 1,
Table 1
Figure BSA0000094091740000061
Figure BSA0000094091740000071
The product 211QL18 culture technique main points that the present invention relates to incubation are as follows:
Through test, plant experimentally, feature and the output of 211QL18 per mu yield more than 200 kilograms forms: the 211QL018 plant height is the 160cm left and right, branch position 40cm left and right, stem branch constitutional balance, once effectively branch amount 8-10 is individual, the effective branch amount 3-5 of secondary, the long 40cm of main shaft left and right, it is with the obvious advantage that individual plant is effectively tied the angle number, reach 400 left and right, Pod length 7cm left and right, every seed number per pod is more than 20, 211QL18 belongs to ripe two low cabbage type rape hybrids in semiwinterness, individual plant effective angle fruit number is many, should grasp rational close planting in cultivation, reach seedling and intertill to loosen the soil in time and eliminate a short early morning, improve Population Quality, accomplish fluently the high yield basis, guaranteeing on the basis of suitable Basic Seedling, promote angle fruit number to grow, thereby reach the many and balance of angle fruit, the purpose that grain is striven for high yield greatly,
Culture technique main points of the present invention are generally similar with No. 10, Qin oil:
One, proper seeding was sowed about September 15, and the seedbed amount of broadcasting 0.5-0.6 kg/acre accomplishes light seeding, even broadcasting, and guaranteed to transplant strong sprout, and the rear timely thinning of emerging, about 40 days length of time rice seedlings grow; 2, cultivate the strong sprout seedbed and be controlled at 1 with the land for growing field crops ratio: the 5-6 left and right, use sufficient base fertilizer, use boron fertilizer and phosphorus potash fertilizer, 3, the rational close planting optimum period transplants, density rests in 0.8-1.0 ten thousand/mu, and live density is 1.2-1.3 ten thousand/mu; 4, every mu of rich water quality management is executed purity nitrogen 15-20 kilogram, heavy dressing base manure, suitably early execute and carry seed manure and tongue fertilizer, and wherein base manure should account for 60% of total fertilizing amount, and guarantee that N, P, K, B coordinate, early execute and carry seed manure after transplanting alive, guarantee to survive the winter strong sprout, at the beginning of late January or 2 months, use tongue fertilizer in the time of the high 2-3 of tongue centimetre, 5, the weed growth of the oily whole season of growth of Lay is controlled in the disease pest and weed control, and anti-eliminating aphis and cabbage caterpillar, sprayed medicine and prevented and treated stalk break seedling stage;
Two, technical essential is implemented in the production of hybrid seeds
1, meticulous sowing, guarantee neat, strong sprout, during the sowing of field Parent, will prevent that interlacing from mixing, and dredges in time, thinning, guarantees neat seedling strong sprout; Select suitable big or small ground porose (preventing the stain evil) basin cylinder, the basin stoneware clay will be take fertilizer as the main base manure of doing, and is later than the sowing of 5-7 days field sowing date, guarantees soil moisture content;
2, pluck in time top, place the basin cylinder: the maternal employing of male sterile line plucked top and processed, promote that secondary divides branch growth and quantity, improve hybrid seed yield, pluck top and be controlled at initial bloom stage first about 7 days, early unfavorable individual plant nourish and grow, unfavorable secondary divides branch growth late; Place the basin cylinder of the male parent of growing on spare row, at other, the basin cylinder is placed on suitable ground, space simultaneously, increases the male parent amount;
3, strengthen management: according to Parent flowering habit especially full-bloom stage Encounter Time, Parent is carried out to the rich water regulation and control: the fertile N fertilizer of suitable Shaoshi that full-bloom stage is slow, coordinate soil moisture content to control, full-bloom stage early do reverse operation, full-bloom stage 11 left and right every morning and about 2 pm with respectively air blast about 5 minutes of small blast fan, enlarge pollen transmission, whether highly need to determine to improve the male parent height by brick on basin cylinder bottom face pad according to female parent; The whole florescence is removed the basin cylinder of isolation mosquito net and placement in time, to strengthen the warm light ventilation condition of system (numerous) plant;
4, in time operation: full-bloom stage 11 left and right every morning and about 2 pm with small blast fan respectively air blast about 5 minutes, enlarge pollen transmission, according to female parent height brick on basin cylinder bottom face pad, improve the male parent height; The whole florescence is removed the basin cylinder of isolation mosquito net and placement, the warm light ventilation condition of planting maternal plant to strengthen system (numerous) in time; After ripe, harvest-seed extraction airing in time, prevent mechanical admixture.
The present invention relates to combine 211QL18 result of the test and technology implementation result
1. result of the test and yield level
In 2009-2010 year combination output approval test, 213.2 kilograms of per mu yields, than No. 10 volume increase 6.20% of contrast Qin oil, in 2010-2011 year variety comparative test, 196.83 kilograms of per mu yields, than 200 kilograms of the general per mu yields of No. 10 volume increase 5.42%, 211QL18 of contrast Qin oil, the high yield field can reach more than 250 kilograms, 2011 start, in Jiangsu, the how plantation that presents a demonstration, paid per unit area yield reaches more than 220 kilograms, yield potential is large;
2, technology implementation result
2011-2012 year adopts this invention technology to carry out male sterile line and cross breeding seed to a plurality of combinations at Jiangsu scientific and technical innovation centre base, result shows, use technical method of the present invention, process is easy, use that material is simple and easy to be easy to get, operating process is easily grasped, saving of labor's abridged edition, and male sterile line or purity of hybrid easily are guaranteed; Height adjusting and the rich water regulation measure of basin cylinder are simple and easy to do, can effectively regulate the Parent flower synchronization time and improve Pollination Effect, overcome tradition with honeybee pass part, artificial pollination loaded down with trivial details and shortcoming that cost is high, hybrid seed yield increases production more than 30% than conventional method, production of hybrid seeds amount easily is protected, the seed obtained is full, and germination rate is high, and follow-up test easily is protected.
Excellent beneficial effect of the present invention:
1, quality is two low, and full seed is perfectly round, and thousand kernel weight is high, reach 3.96g (general cross rape thousand kernel weight is the 3.7g left and right), seed is black, and commodity is good, Ministry of Agriculture's quality testing center test result is delivered in sampling in 2011: oil content 40.20%, glucosinolate content 14.36umol/g cake powder, content of erucic acid 0.1%, meet national double-low rapeseed standard, stalk break is lighter, the incidence of disease 5.83%, disease refers to 2.96, cold resistance is good;
2, resistance is outstanding, high and stable yield is good, the rape individuality the present invention relates to all has well anti-(anti-) property to stalk break and virus disease, through Institute of Plant Protection, academy of agricultural sciences, Jiangsu Province, unified sampling is identified, stalk break is lighter, the incidence of disease 5.83%, disease refers to 2.96, the performance high resistance, other mixed natural disposition diseases are light, waterlogging tolerance is good, seedling stage is upright, tongue phase robust growth, spring is sent out by force, florescence concentrates, grain closeer, structure of output is coordinated, ripe uniformity is better, general 210 kilograms of per mu yields (the high yield field can reach more than 250 kilograms), contrast No. 7 volume increase 6% left and right of Qin's oil, plant height 158.5cm, several 8.97 of primary branch, 3.78 of secondary branch amounts, 331.22 of the effective branch amounts of complete stool, angular length 7.28cm, every seed number per pod is more, it is 20.63, Self-regulation ability is strong, easily cultivation is promoted, individual seedling stage is upright, tongue phase robust growth, spring is sent out by force, florescence concentrates, grain closeer, structure of output is coordinated, ripe uniformity is better,
3, production of hybrid seeds process safety facility, use technical method of the present invention, process is easy, use that material is simple and easy to be easy to get, processing ease is grasped, saving of labor's abridged edition: the isolation yarn account of use and small blast fan, the basin cylinder added are easily purchased on market, the height of basin cylinder regulate and the rich water regulation measure simple and easy to do, overcome in general small size production of hybrid seeds process place that beehive uses that honeybee passes part, artificial pollination loaded down with trivial details and shortcoming that cost is high;
4, efficient, the high yield of the production of hybrid seeds, whole process is only carried out simple operations in the critical period, it is simple and easy to do that the whole florescence is removed the basin cylinder of placing, male parent without coming off, produce mix may, product male sterile line or the purity of hybrid of preparation easily are guaranteed; Can obtain maximum pollination face and the longest pollination time during flower synchronization, output easily is protected, and the seed obtained is full, and germination rate is high, and follow-up test easily is protected.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any modifications of doing within the spirit and principles in the present invention, be equal to and replace and improvement etc., within all should being included in protection scope of the present invention.

Claims (3)

1. a three-tine hybrid rape combination producing method for seed, is characterized in that, this three-tine hybrid rape combination producing method for seed comprises the following steps:
Select Brassica napus male sterile line as female parent, cabbage type rape hybrid maintainer town B152 makes male parent, by hybridizing for many years transformation, obtains Brassica napus male sterile line town A152;
It is maternal selecting Brassica napus male sterile line, with cabbage type rape, makes male parent, by hybridizing for many years transformation, obtains cabbage type rape restorer R023; The mixed planting of Parent branch, maturing stage results cabbage type rape hybrid seed;
Maintainer town B152 is stablized in utilization and sterile line propagation is carried out in A152 hybridization in male sterile line town, utilizes strong restorer R023 and male sterile line town A152 to carry out the hybrid seed production of hybrid seeds.
2. three-tine hybrid rape combination producing method for seed as claimed in claim 1, is characterized in that, in this three-tine hybrid rape combination producing method for seed, production of hybrid seeds type of seeding is: every community production of hybrid seeds area 20m 2, surrounding plantation male parent, Parent is planted altogether in the middle of 6 Hang, communities and stayed 1 spare row, Parent plantation is sequentially 1-2-0-2-1, line-spacing 35cm, male parent spacing in the rows 20cm, maternal spacing in the rows 15cm.
3. three-tine hybrid rape as claimed in claim 1 combines producing method for seed, it is characterized in that, in this three-tine hybrid rape combination producing method for seed, producing method for seed is: the isolation yarn account that long 6.7m for production of hybrid seeds community * wide 3.0m * high 3.0m nonwoven is made covers, the potted plant male parent of spare row, the maternal employing of male sterile line plucked to the top processing, promote that secondary divides branch growth and quantity.
CN 201310363314 2013-08-16 2013-08-16 Combined seed production method for three-line hybrid oilseed rape Pending CN103461097A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105850718A (en) * 2016-04-20 2016-08-17 江西农业大学 Method for prolonging rapeseed flowering period and preparation method of sterile rapeseed
CN106857128A (en) * 2017-02-16 2017-06-20 贵州红四方农业发展股份有限公司 A kind of hybrid rice male parent rice transplanting method
CN108207615A (en) * 2018-01-12 2018-06-29 德宏州农业技术推广中心(德宏州农业科学研究所) A kind of three incross rapeseed method

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105850718A (en) * 2016-04-20 2016-08-17 江西农业大学 Method for prolonging rapeseed flowering period and preparation method of sterile rapeseed
CN106857128A (en) * 2017-02-16 2017-06-20 贵州红四方农业发展股份有限公司 A kind of hybrid rice male parent rice transplanting method
CN108207615A (en) * 2018-01-12 2018-06-29 德宏州农业技术推广中心(德宏州农业科学研究所) A kind of three incross rapeseed method
CN108207615B (en) * 2018-01-12 2019-03-19 德宏州农业技术推广中心(德宏州农业科学研究所) A kind of three incross rapeseed method

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