CN103222540B - Composite micro-ecological fish feed additive - Google Patents
Composite micro-ecological fish feed additive Download PDFInfo
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- CN103222540B CN103222540B CN201310153178.5A CN201310153178A CN103222540B CN 103222540 B CN103222540 B CN 103222540B CN 201310153178 A CN201310153178 A CN 201310153178A CN 103222540 B CN103222540 B CN 103222540B
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Abstract
The invention relates to a composite micro-ecological fish feed additive composed of materials, a composite bacteria liquid, prebiotics, and sterile water. The addition amount of the composite bacteria liquid is 0.15% of that of the materials; the addition amount of prebiotics is 1.0% of that of the materials; and the addition amount of the sterile water is 35-50% of that of the materials. In the composite micro-ecological fish feed additive, the content of prebiotics exceeds 1011cfu/g, and the prebiotics produce large amounts of proteases, amylases, lipases, and peptides. The strains are processed through temperature acclimation, such that thermal stability is high. Three prebiotic polysaccharides are added, such that viability of the prebiotics in fish intestines is improved, and probiotic loss rate is below 10%. In fish stool, probiotic content is greatly increased, such that water body can be cleaned, and fish production cost can be reduced.
Description
Technical field
The present invention relates to aquaculture feed additive technical field, be specifically related to a kind of compound microecological fish feed additive.
Background technology
In recent years, people very pay close attention to dietetic hygiene and medicament residue, they have badly influenced people's health, from source, pick up, naturally various animal feeds have just been related to, in Europe, some countries have had realized that this point in 19th century, and they have carried out strict control to feed addictive, and for example Denmark forbids to add antibiotics in feed. so micro-ecological feed has obtained using widely abroad.
Fish products consumption figure is at home very large, so the green intensity of fish feed is directly connected to people's health, and still using antibiotics additive in a lot of fish products plants, and compound microecological fish feed additive can address this problem, substitute antibiotics completely, realize and improve fish immunity power, reduce disease, and the prebiotics in compound formulation can promote the gut flora balance of fish, promote Metabolism of Fishes growth, improve output, can also solve the problem of fish manure contamination water body, probio in compound formulation can be improved water body environment, reduced production cost. therefore, composite microorganism type forage additives is the trend of feed addictive application development.
Summary of the invention
The object of the invention is the deficiency for solving the problems of the technologies described above, a kind of compound microecological fish feed additive and preparation technology thereof are provided, and closing micro-ecological fish feedstuff additive product can substitute antibiotics, improves fish immunity power, improve water body environment, promote fish growth.
The present invention is the deficiency solving the problems of the technologies described above, the technical scheme adopting is: a kind of compound microecological fish feed additive, by material, composite bacteria liquid, prebiotics and sterilized water, formed, wherein: the addition of composite bacteria liquid is weight of material 1.5 ‰, the addition of prebiotics is 1.0% of weight of material, and the addition of sterilized water is weight of material 35～50%;
Described material is comprised of 1～2 part, 10～25 parts of fish meal, 40～60 parts of bean cake powders, 30～45 parts, wheat bran, 25～35 parts of corn flour, 1～3 part of vegetable oil and sodium chloride by weight;
Described composite bacteria liquid by weight by producing 0.3～1.0 part of protease bacillus subtilis, produce 0.3～1.0 part of amylase bacillus subtilis, 0.8～1.5 part and 1.5～2.5 parts of 0.3～1.0 part of yielding lipase bacillus subtilis, 0.5～1.0 part of bacillus cereus, 1.0～1.8 parts of enterococcus faecalis, 1.8～2.5 parts of candida utili, 0.8～1.5 part of geotrichum candidum, Bifidobacterium form;
Described prebiotics is comprised of 5～15 parts of 3～10 parts of FOSs, 6～12 parts of galactooligosaccharides and Oligomeric manna sugars by weight.
Described compound microecological fish feed additive adds in fish meal according to the ratio of percentage by weight 5%～9%.
A preparation technology for compound microecological fish feed additive, comprises the following steps:
1), get by ratio of weight and the number of copies 1～2 part, 10～25 parts of fish meal, 40～60 parts of bean cake powders, 30～45 parts, wheat bran, 25～35 parts of corn flour, 1～3 part of vegetable oil and sodium chloride, solid material is carried out to pulverization process, and mistake 80 mesh sieves, then after each component being mixed, stir, obtain material, standby;
2), utilize conventional method to cultivate separately the required bacterial classification of composite bacteria liquid, and get by weight and produce 0.3～1.0 part of protease bacillus subtilis, produce 0.3～1.0 part of amylase bacillus subtilis, 0.3～1.0 part of yielding lipase bacillus subtilis, 0.5～1.0 part of bacillus cereus, 1.0～1.8 parts of enterococcus faecalis, 1.8～2.5 parts of candida utili, 0.8～1.5 part of geotrichum candidum, 1.5～2.5 parts of 0.8～1.5 part of Bifidobacterium and lactic acid bacterias, first will produce protease bacillus subtilis, produce amylase bacillus subtilis, yielding lipase bacillus subtilis, bacillus cereus, candida utili and geotrichum candidum mix sealing and cultivate 3h, then add enterococcus faecalis, Bifidobacterium and lactic acid bacteria, obtain composite bacteria liquid, standby,
3) get, by ratio of weight and the number of copies 5～15 parts of 3～10 parts of FOSs, 6～12 parts of galactooligosaccharides and Oligomeric manna sugars, after mixing, obtain prebiotics, standby;
4), get the composite bacteria liquid of weight of material 1.5 ‰ and the sterilized water of weight of material 35～50%, sterilized water is added in composite bacteria liquid, stir, obtain dilution composite bacteria liquid; The prebiotics of separately getting weight of material 1.0% is standby;
5), the composite bacteria liquid of dilution that step 4) is obtained adds in the material that step 1) obtains, after stirring, at 35～40 ℃ of temperature, aerobic fermentation is 48～72 hours;
6), after fermentation ends, it is 4%～8% that compound is dried to moisture content under lower than 45 ℃ of conditions, then pulverizes 30 mesh sieves;
7), stir after the compound after pulverizing is mixed with the prebiotics of step 4) taking-up, after packing, obtain compound microecological fish feed additive finished product.
beneficial effect
Compound microecological fish feed additive of the present invention, probio content surpasses 10
11cfu/g, and probio produces a large amount of protease, amylase, lipase, active peptide.And bacterial classification has all been carried out to temperature acclimatisation, and heat endurance is higher; Three kinds of prebiotics polysaccharide have wherein been added, improved the survival ability of probio in fish intestines, make the loss late of probio below 10%, and micro-ecological feed loss late is on the market generally more than 30%, in fish faeces, the content of probio is also greatly improved, can purifying water body, reduce fish production cost.
The specific embodiment
A kind of compound microecological fish feed additive, by material, composite bacteria liquid, prebiotics and sterilized water, formed, wherein: the addition of composite bacteria liquid is weight of material 1.5 ‰, the addition of prebiotics is weight of material 1.0%, and the addition of sterilized water is weight of material 35～50%;
Described material is comprised of 1～2 part, 10～25 parts of fish meal, 40～60 parts of bean cake powders, 30～45 parts, wheat bran, 25～35 parts of corn flour, 1～3 part of vegetable oil and sodium chloride by weight;
Described composite bacteria liquid by weight by producing 0.3～1.0 part of protease bacillus subtilis, produce 0.3～1.0 part of amylase bacillus subtilis, 0.8～1.5 part and 1.5～2.5 parts of 0.3～1.0 part of yielding lipase bacillus subtilis, 0.5～1.0 part of bacillus cereus, 1.0～1.8 parts of enterococcus faecalis, 1.8～2.5 parts of candida utili, 0.8～1.5 part of geotrichum candidum, Bifidobacterium form;
Product protease bacillus subtilis wherein, product amylase bacillus subtilis and yielding lipase bacillus subtilis for to screen respectively the bacterial classification of high proteinase yield, high yield amylase and high yielding lipase from bacillus subtilis, and screening technique is conventional method.
Described prebiotics is comprised of 5～15 parts of 3～10 parts of FOSs, 6～12 parts of galactooligosaccharides and Oligomeric manna sugars by weight.
Described compound microecological fish feed additive adds in fish meal according to the ratio of percentage by weight 5%～9%.
A preparation technology for compound microecological fish feed additive, comprises the following steps:
1), get by ratio of weight and the number of copies 1～2 part, 10～25 parts of fish meal, 40～60 parts of bean cake powders, 30～45 parts, wheat bran, 25～35 parts of corn flour, 1～3 part of vegetable oil and sodium chloride, solid material is carried out to pulverization process, and mistake 80 mesh sieves, then after each component being mixed, stir, obtain material, standby;
2), utilize conventional method to cultivate separately the required bacterial classification of composite bacteria liquid, and get by weight and produce 0.3～1.0 part of protease bacillus subtilis, produce 0.3～1.0 part of amylase bacillus subtilis, 0.3～1.0 part of yielding lipase bacillus subtilis, 0.5～1.0 part of bacillus cereus, 1.0～1.8 parts of enterococcus faecalis, 1.8～2.5 parts of candida utili, 0.8～1.5 part of geotrichum candidum, 1.5～2.5 parts of 0.8～1.5 part of Bifidobacterium and lactic acid bacterias, first will produce protease bacillus subtilis, produce amylase bacillus subtilis, yielding lipase bacillus subtilis, bacillus cereus, candida utili and geotrichum candidum mix sealing and cultivate 3h, then add enterococcus faecalis, Bifidobacterium and lactic acid bacteria, obtain composite bacteria liquid, standby,
3) get, by ratio of weight and the number of copies 5～15 parts of 3～10 parts of FOSs, 6～12 parts of galactooligosaccharides and Oligomeric manna sugars, after mixing, obtain prebiotics, standby;
4), get the composite bacteria liquid of weight of material 1.5 ‰ and the sterilized water of weight of material 35～50%, sterilized water is added in composite bacteria liquid, stir, obtain dilution composite bacteria liquid; The prebiotics of separately getting weight of material 1.0% is standby;
5), the composite bacteria liquid of dilution that step 4) is obtained adds in the material that step 1) obtains, after stirring, at 35～40 ℃ of temperature, aerobic fermentation is 48～72 hours;
6), after fermentation ends, it is 4%～8% that compound is dried to moisture content under lower than 45 ℃ of conditions, then pulverizes 30 mesh sieves;
7), stir after the compound after pulverizing is mixed with the prebiotics of step 4) taking-up, after packing, obtain compound microecological fish feed additive finished product.
Below specific embodiments of the invention:
embodiment 1
A preparation method for compound microecological fish feed additive, comprises the following steps:
1), get by ratio of weight and the number of copies 1 part, 10 parts of fish meal, 40 parts of bean cake powders, 30 parts, wheat bran, 25 parts of corn flour, 1 part of vegetable oil and sodium chloride, solid material is carried out to pulverization process, and cross 80 mesh sieves, after then each component being mixed, stir, obtain material, standby;
2), utilize conventional method to cultivate separately the required bacterial classification of composite bacteria liquid, and get by weight and produce 0.3 part of protease bacillus subtilis, produce 0.3 part of amylase bacillus subtilis, 0.3 part of yielding lipase bacillus subtilis, 0.5 part of bacillus cereus, 1.0 parts of enterococcus faecalis, 1.8 parts of candida utili, 0.8 part of geotrichum candidum, 1.5 parts of 0.8 part of Bifidobacterium and lactic acid bacterias, first will produce protease bacillus subtilis, produce amylase bacillus subtilis, yielding lipase bacillus subtilis, bacillus cereus, candida utili and geotrichum candidum mix sealing and cultivate 3h, then add enterococcus faecalis, Bifidobacterium and lactic acid bacteria, obtain composite bacteria liquid, standby,
3) get, by ratio of weight and the number of copies 5 parts of 3 parts of FOSs, 6 parts of galactooligosaccharides and Oligomeric manna sugars, after mixing, obtain prebiotics, standby;
4), get the composite bacteria liquid of weight of material 1.5 ‰ and the sterilized water of weight of material 35%, sterilized water is added in composite bacteria liquid, stir, obtain dilution composite bacteria liquid; The prebiotics of separately getting weight of material 1.0% is standby;
5), the composite bacteria liquid of dilution that step 4) is obtained adds in the material that step 1) obtains, after stirring, at 35～40 ℃ of temperature, aerobic fermentation is 48 hours;
6), after fermentation ends, it is 4%～8% that compound is dried to moisture content under lower than 45 ℃ of conditions, then pulverizes 30 mesh sieves;
7), stir after the compound after pulverizing is mixed with the prebiotics of step 4) taking-up, after packing, obtain compound microecological fish feed additive finished product.
embodiment 2
A preparation method for compound microecological fish feed additive, comprises the following steps:
1), get by ratio of weight and the number of copies 2 parts, 25 parts of fish meal, 60 parts of bean cake powders, 45 parts, wheat bran, 35 parts of corn flour, 3 parts of vegetable oil and sodium chloride, solid material is carried out to pulverization process, and cross 80 mesh sieves, after then each component being mixed, stir, obtain material, standby;
2), utilize conventional method to cultivate separately the required bacterial classification of composite bacteria liquid, and get by weight and produce 1.0 parts of protease bacillus subtilises, produce 1.0 parts of amylase bacillus subtilises, 1.0 parts of yielding lipase bacillus subtilises, 1.0 parts of bacillus cereus, 1.8 parts of enterococcus faecalis, 2.5 parts of candida utili, 1.5 parts of geotrichum candidums, 2.5 parts of 1.5 parts of Bifidobacteriums and lactic acid bacterias, first will produce protease bacillus subtilis, produce amylase bacillus subtilis, yielding lipase bacillus subtilis, bacillus cereus, candida utili and geotrichum candidum mix sealing and cultivate 3h, then add enterococcus faecalis, Bifidobacterium and lactic acid bacteria, obtain composite bacteria liquid, standby,
3) get, by ratio of weight and the number of copies 15 parts of 10 parts of FOSs, 12 parts of galactooligosaccharides and Oligomeric manna sugars, after mixing, obtain prebiotics, standby;
4), get the composite bacteria liquid of weight of material 1.5 ‰ and the sterilized water of weight of material 50%, sterilized water is added in composite bacteria liquid, stir, obtain dilution composite bacteria liquid; The prebiotics of separately getting weight of material 1.0% is standby;
5), the composite bacteria liquid of dilution that step 4) is obtained adds in the material that step 1) obtains, after stirring, at 35～40 ℃ of temperature, aerobic fermentation is 72 hours;
6), after fermentation ends, it is 4%～8% that compound is dried to moisture content under lower than 45 ℃ of conditions, then pulverizes 30 mesh sieves;
7), stir after the compound after pulverizing is mixed with the prebiotics of step 4) taking-up, after packing, obtain compound microecological fish feed additive finished product.
embodiment 3
A preparation method for compound microecological fish feed additive, comprises the following steps:
1), get by ratio of weight and the number of copies 1.5 parts, 15 parts of fish meal, 45 parts of bean cake powders, 35 parts, wheat bran, 30 parts of corn flour, 2 parts of vegetable oil and sodium chloride, solid material is carried out to pulverization process, and cross 80 mesh sieves, after then each component being mixed, stir, obtain material, standby;
2), utilize conventional method to cultivate separately the required bacterial classification of composite bacteria liquid, and get by weight and produce 0.6 part of protease bacillus subtilis, produce 0.7 part of amylase bacillus subtilis, 0.5 part of yielding lipase bacillus subtilis, 0.9 part of bacillus cereus, 1.5 parts of enterococcus faecalis, 2.2 parts of candida utili, 1.2 parts of geotrichum candidums, 2.0 parts of 1.0 parts of Bifidobacteriums and lactic acid bacterias, first will produce protease bacillus subtilis, produce amylase bacillus subtilis, yielding lipase bacillus subtilis, bacillus cereus, candida utili and geotrichum candidum mix sealing and cultivate 3h, then add enterococcus faecalis, Bifidobacterium and lactic acid bacteria, obtain composite bacteria liquid, standby,
3) get, by ratio of weight and the number of copies 10 parts of 7 parts of FOSs, 9 parts of galactooligosaccharides and Oligomeric manna sugars, after mixing, obtain prebiotics, standby;
4), get the composite bacteria liquid of weight of material 1.5 ‰ and the sterilized water of weight of material 40%, sterilized water is added in composite bacteria liquid, stir, obtain dilution composite bacteria liquid; The prebiotics of separately getting weight of material 1.0% is standby;
5), the composite bacteria liquid of dilution that step 4) is obtained adds in the material that step 1) obtains, after stirring, at 37 ℃ of temperature, aerobic fermentation is 60 hours;
6), after fermentation ends, it is 4%～8% that compound is dried to moisture content under lower than 45 ℃ of conditions, then pulverizes 30 mesh sieves;
7), stir after the compound after pulverizing is mixed with the prebiotics of step 4) taking-up, after packing, obtain compound microecological fish feed additive finished product.
embodiment 4
A preparation method for compound microecological fish feed additive, comprises the following steps:
1), get by ratio of weight and the number of copies 1 part, 25 parts of fish meal, 60 parts of bean cake powders, 30 parts, wheat bran, 35 parts of corn flour, 3 parts of vegetable oil and sodium chloride, solid material is carried out to pulverization process, and cross 80 mesh sieves, after then each component being mixed, stir, obtain material, standby;
2), utilize conventional method to cultivate separately the required bacterial classification of composite bacteria liquid, and get by weight and produce 1.0 parts of protease bacillus subtilises, produce 1.0 parts of amylase bacillus subtilises, 0.4 part of yielding lipase bacillus subtilis, 0.5 part of bacillus cereus, 1.6 parts of enterococcus faecalis, 1.9 parts of candida utili, 1.3 parts of geotrichum candidums, 1.8 parts of 1.2 parts of Bifidobacteriums and lactic acid bacterias, first will produce protease bacillus subtilis, produce amylase bacillus subtilis, yielding lipase bacillus subtilis, bacillus cereus, candida utili and geotrichum candidum mix sealing and cultivate 3h, then add enterococcus faecalis, Bifidobacterium and lactic acid bacteria, obtain composite bacteria liquid, standby,
3) get, by ratio of weight and the number of copies 9 parts of 6 parts of FOSs, 6 parts of galactooligosaccharides and Oligomeric manna sugars, after mixing, obtain prebiotics, standby;
4), get the composite bacteria liquid of weight of material 1.5 ‰ and the sterilized water of weight of material 45%, sterilized water is added in composite bacteria liquid, stir, obtain dilution composite bacteria liquid; The prebiotics of separately getting weight of material 1.0% is standby;
5), the composite bacteria liquid of dilution that step 4) is obtained adds in the material that step 1) obtains, after stirring, at 40 ℃ of temperature, aerobic fermentation is 55 hours;
6), after fermentation ends, it is 4%～8% that compound is dried to moisture content under lower than 45 ℃ of conditions, then pulverizes 30 mesh sieves;
7), stir after the compound after pulverizing is mixed with the prebiotics of step 4) taking-up, after packing, obtain compound microecological fish feed additive finished product.
Claims (1)
1. a preparation technology for compound microecological fish feed additive, is characterized in that: comprise the following steps:
1), get by ratio of weight and the number of copies 1～2 part, 10～25 parts of fish meal, 40～60 parts of bean cake powders, 30～45 parts, wheat bran, 25～35 parts of corn flour, 1～3 part of vegetable oil and sodium chloride, solid material is carried out to pulverization process, and mistake 80 mesh sieves, then after each component being mixed, stir, obtain material, standby;
2), utilize conventional method to cultivate separately the required bacterial classification of composite bacteria liquid, and get by weight and produce 0.3～1.0 part of protease bacillus subtilis, produce 0.3～1.0 part of amylase bacillus subtilis, 0.3～1.0 part of yielding lipase bacillus subtilis, 0.5～1.0 part of bacillus cereus, 1.0～1.8 parts of enterococcus faecalis, 1.8～2.5 parts of candida utili, 0.8～1.5 part of geotrichum candidum, 1.5～2.5 parts of 0.8～1.5 part of Bifidobacterium and lactic acid bacterias, first will produce protease bacillus subtilis, produce amylase bacillus subtilis, yielding lipase bacillus subtilis, bacillus cereus, candida utili and geotrichum candidum mix sealing and cultivate 3h, then add enterococcus faecalis, Bifidobacterium and lactic acid bacteria, obtain composite bacteria liquid, standby,
3) get, by ratio of weight and the number of copies 5～15 parts of 3～10 parts of FOSs, 6～12 parts of galactooligosaccharides and Oligomeric manna sugars, after mixing, obtain prebiotics, standby;
4), get the composite bacteria liquid of weight of material 1.5 ‰ and the sterilized water of weight of material 35～50%, sterilized water is added in composite bacteria liquid, stir, obtain dilution composite bacteria liquid; The prebiotics of separately getting weight of material 1.0% is standby;
5), the composite bacteria liquid of dilution that step 4) is obtained adds in the material that step 1) obtains, after stirring, at 35～40 ℃ of temperature, aerobic fermentation is 48～72 hours;
6), after fermentation ends, it is 4%～8% that compound is dried to moisture content under lower than 45 ℃ of conditions, then pulverizes 30 mesh sieves;
7), stir after the compound after pulverizing is mixed with the prebiotics of step 4) taking-up, after packing, obtain compound microecological fish feed additive finished product.
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104770596A (en) * | 2015-04-07 | 2015-07-15 | 中国水产科学研究院珠江水产研究所 | Aquatic animal intestinal regulator and application thereof |
| CN105876256A (en) * | 2016-04-13 | 2016-08-24 | 江苏星海生物科技有限公司 | Fish feed rich in nutrition and preparation method thereof |
| CN105961909A (en) * | 2016-05-04 | 2016-09-28 | 武汉水之国环保科技有限公司 | Stingray feed additive |
| CN107156541A (en) * | 2017-06-29 | 2017-09-15 | 天津海友佳音生物科技股份有限公司 | A kind of water adjusting agent used for aquiculture |
| CN109179685A (en) * | 2018-09-14 | 2019-01-11 | 广东渔夫宝水产科技有限公司 | A kind of synbiotic and its application method for aquaculture pool head viable bacteria fermentation |
| CN112293605A (en) * | 2019-07-25 | 2021-02-02 | 领袖生物技术农业会社法人 | EP puffed fermented compound feed with effects of promoting digestion, immunocompetence and growth of fish and preparation method thereof |
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| CN101248841A (en) * | 2008-03-31 | 2008-08-27 | 天津天农康嘉生态养殖有限公司 | Feedstuff additive nutriment |
| CN101878858A (en) * | 2010-06-23 | 2010-11-10 | 南京农业大学 | Microecological composite growth-promoting feed additive for fish |
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| EP1472933A1 (en) * | 2003-05-01 | 2004-11-03 | Chr. Hansen A/S | Improving health and/or nutritional status of an aquatic animal by aid of Bacillus licheniformis |
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