CN103205375A - Quality clostridium butyricum for breeding egg-laying poultry - Google Patents
Quality clostridium butyricum for breeding egg-laying poultry Download PDFInfo
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- CN103205375A CN103205375A CN2013100839002A CN201310083900A CN103205375A CN 103205375 A CN103205375 A CN 103205375A CN 2013100839002 A CN2013100839002 A CN 2013100839002A CN 201310083900 A CN201310083900 A CN 201310083900A CN 103205375 A CN103205375 A CN 103205375A
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- clostridium
- clostridium butyricum
- clostridium butylicum
- egg
- butylicum
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Abstract
Disclosed is quality clostridium butyricum for breeding egg-laying poultry. The quality clostridium butyricum is capable of promoting growth and development of livestock and poultry, increasing the utilization rate of feed, reducing the mortality rate of young livestock and poultry and improving the performance of the egg-laying poultry. The technical scheme includes that the clostridium butyricum HM-68 is preserved in the China General Microbiological Culture Collection Center on 29th October, 2012, and the preservation number of the clostridium butyricum is CGMCC No.6732. The invention further discloses an application method for the clostridium butyricum.
Description
Technical field
The present invention relates to a strain clostridium butylicum, specifically is the clostridium butylicum that egg fowl is cultured that is used for that a strain can promote animal growth and development, improve efficiency of feed utilization, reduces cub poult mortality ratio and improve egg fowl production performance.
Background technology
For a long time, people adopt always and add disease and the raising production performance that antibiosis is usually controlled livestock and poultry in feeds.Because a large amount of uses of microbiotic, hormone descend egg, meat, the milk quality of livestock and poultry, long-term eating brought potential hazard to human health.Therefore, European Union forbade adding microbiotic feed from 1999.21 century enters into the epoch of a promotion " healthy, green ", and the health that various industries have all begun with humans and animals is the green of core, the research of ecology.Livestock industry has also responded the slogan of " healthy, green " as mainstay industry of China after having experienced 20th century flourish, enter a brand-new stage.And probiotics has been obtained very big achievement in practice as the relatively more outstanding class of effect.
Clostridium butyricum active bacteria preparation in the market is all from Japanese import, and price is higher, and clostridium butylicum has wide application prospect as medicine, protective foods, fodder additives and microbial fertilizer.If clostridium butylicum as fodder additives, can make our livestock products develop towards green direction, whole livestock industry and feed already there is profound significance.
The present invention is directed to above-mentioned problem, develop a kind of good clostridium butylicum HM-68(Clostridium butyricum HM-68 of livestock and poultry cultivation), as fodder additives, it can promote animal growth and development, improves efficiency of feed utilization, improves the animal digestive tract micro-ecological environment, promote intestinal health, regulate body immunity, reduce cub poult mortality ratio, improve advantages such as egg fowl production performance.
Summary of the invention
The good clostridium butylicum that egg fowl is cultured that is used for that the object of the present invention is to provide a strain can promote animal growth and development, improves efficiency of feed utilization, reduces cub poult mortality ratio and improve egg fowl production performance.
The present invention is achieved through the following technical solutions.
One strain is used for the good clostridium butylicum that egg fowl is cultured, it is characterized in that: described clostridium butylicum HM-68(Clostridium butyricum HM-68) on October 29th, 2012, be Da Tun road, Chaoyang District, BeiJing, China city in the address, China Committee for Culture Collection of Microorganisms common micro-organisms center (State Patent Office specifies patent microbial preservation center) preservation, preserving number: CGMCC No.6732.
Clostridium butyricum HM-68 separates in the healthy chicken enteron aisle, clostridium butylicum (Clostridiumbutyricum HM-68) can be in animal intestinal the antagonism animal pathogen, promote animal growth and development, improve efficiency of feed utilization, improve the animal digestive tract micro-ecological environment, promote intestinal health, regulate body immunity, reduce cub poult mortality ratio.
A kind of application of clostridium butylicum is characterized in that: be used for improving egg fowl and culture.
A kind of activation of clostridium butylicum and cultivation, it is characterized in that: the Clostridiumbutyricum HM-68 of freezing preservation is inoculated in the GPY liquid nutrient medium, cultivate 20h down for 37 ℃ in temperature, so going down to posterity to cultivate obtains described activation Clostridium butyricum HM-68 bacterial classification 2-3 time; Described GPY liquid nutrient medium is composed as follows: peptone 10.0g, yeast extract paste 10.0g, glucose 6.0g, MgSO
47H
2O1.0g, K
2HPO
42.0g, MnSO
450ppm adds 1L distilled water, regulates pH to 6.8,121 ℃ of sterilization 15min.
A kind of preparation of clostridium butylicum bacterium powder is characterized in that the bacterium powder is made in bacterial classification inoculation fermentation, drying, the pulverizing of activation, makes its viable count reach 1 * 10
9Cfu/g.
The using method of a kind of clostridium butylicum in feed is characterized in that: the bacterium powder after strain fermentation, freeze-drying, the pulverizing and basal diet are stirred stand-by, the clostridium butyricum active bacteria number reaches 1 * 10 in the feed
5Cfu/g.
The application of described clostridium butylicum aspect livestock and poultry cultivation, experimental technique is as follows:
Testing program: select 360 of the brown laying hens of 34 healthy commodity Dai Hailan in age in week, adopt and singly have the repeated in experiments design, be divided into 6 processing at random, each handles 60 chickens, per 3 chickens are 1 repetition, be that each treatment group can obtain 20 repeated observation data, 42 days test periods, be divided equally into control group and test group; Control group fed typical case corn dregs of beans type basal diet, test group is fed and is added the basal diet of clostridium butylicum.
Index determining: duration of test is pressed duplicate record egg number, egg size, food consumption and is extremely washed in a pan the chicken number every day, and laying rate, average egg weight, feedstuff-egg ratio and death rate are calculated in off-test.
The experimental animal management: test chicken adopts staged to raise in cages natural ventilation, free choice feeding and drinking-water; Immunity and expelling parasite program are carried out synchronously by the chicken house routine.In time receiving tank external loss material is put back in the groove, observes at any time, records that chicken searches for food and healthy state.
Clostridium butylicum provided by the invention has following positively effect:
Experimental result shows that the clostridium butyricum active bacteria number reaches 1 * 10 in the feed
5During cfu/g, the laying hen of feeding can significantly reduce feedstuff-egg ratio, saves feed intake, improves the economic benefit that laying hen produces.
Test-results shows that the clostridium butyricum active bacteria number reaches 1 * 10 in the feed
5During cfu/g, can be in animal intestinal behind the laying hen of feeding the antagonism pathogenic bacteria, keep and regulate the intestinal microecology balance, strengthen the animal body immunologic function, significantly reduce the laying hen death rate.
Embodiment
The invention will be further described below in conjunction with embodiment; Following embodiment is illustrative, does not limit protection scope of the present invention.
Experimental technique among the following embodiment if no special instructions, is ordinary method.
One strain is used for the good clostridium butylicum that egg fowl is cultured, described clostridium butylicum HM-68(Clostridiumbutyricum HM-68) on October 29th, 2012, be Da Tun road, Chaoyang District, BeiJing, China city in the address, China Committee for Culture Collection of Microorganisms common micro-organisms center (State Patent Office specifies patent microbial preservation center) preservation, preserving number: CGMCC No.6732.
Clostridium butyricum HM-68 separates in the healthy chicken enteron aisle, clostridium butylicum (Clostridiumbutyricum HM-68) can be in animal intestinal the antagonism animal pathogen, promote animal growth and development, improve efficiency of feed utilization, improve the animal digestive tract micro-ecological environment, promote intestinal health, regulate body immunity, reduce cub poult mortality ratio.
A kind of application of clostridium butylicum is used for improving egg fowl and cultures.
A kind of activation of clostridium butylicum and cultivation, the Clostridium butyricum HM-68 of freezing preservation is inoculated in the GPY liquid nutrient medium, cultivate 20h down for 37 ℃ in temperature, so going down to posterity to cultivate obtains described activation Clostridium butyricum HM-68 bacterial classification 2-3 time; Described GPY liquid nutrient medium is composed as follows: peptone 10.0g, yeast extract paste 10.0g, glucose 6.0g, MgSO
47H
2O1.0g, K
2HPO
42.0g, MnSO
450ppm adds 1L distilled water, regulates pH to 6.8,121 ℃ of sterilization 15min.
A kind of preparation of clostridium butylicum bacterium powder is made the bacterium powder with bacterial classification inoculation fermentation, drying, the pulverizing of activation, makes its viable count reach 1 * 10
9Cfu/g.
The using method of a kind of clostridium butylicum in feed stirs the bacterium powder after strain fermentation, freeze-drying, the pulverizing and basal diet stand-by, and the clostridium butyricum active bacteria number reaches 1 * 10 in the feed
5Cfu/g.
Experiment 1: the situation of reduction laying hen feedstuff-egg ratio clostridium butylicum HM-68(Clostridium butyricum HM-68).
Select 360 of the brown laying hens of 34 healthy commodity Dai Hailan in age in week, adopt single-factor that the repeated in experiments design is arranged, be divided into 6 processing at random, each handles 60 chickens, per 3 chickens are 1 repetition, and namely each treatment group can obtain 20 repeated observation data, 42 days test periods, be divided equally into control group and test group, handle 1 group for per 3.Basal diet prescription and trophic level see Table 1.Every day, laying rate, average egg weight were calculated in off-test by duplicate record egg number, egg size.
Control group fed typical case corn dregs of beans type basal diet, test group is fed and is added the basal diet of clostridium butylicum, and Clostridium butyricum HM-68 viable count reaches 1 * 10 in every gram feed
5Cfu/g.Laying rate of laying hen, average egg weight situation see Table 2.
Table 1 basal diet prescription and trophic level
Table 2 laying rate of laying hen, average egg weight situation
By table 2 test-results as can be known, Clostridium butyricum HM-68 viable count reaches 1 * 10 in the feed
5Cfu/g, laying rate of laying hen significantly improves behind the laying hen of feeding, the average egg weight indifference.
Experiment 2: clostridium butylicum HM-68(Clostridium butyricum HM-68) laying hen feedstuff-egg ratio, death rate are improved situation.
Select 360 of the brown laying hens of 34 healthy commodity Dai Hailan in age in week, adopt and singly have the repeated in experiments design, be divided into 6 processing at random, each handles 60 chickens, per 3 chickens are 1 repetition, and namely each treatment group can obtain 20 repeated observation data, 42 days test periods, be divided into control group and test group, handle 1 group for per 3.Every day by duplicate record lay eggs heavily, food consumption and extremely wash in a pan the chicken number, feedstuff-egg ratio and death rate are calculated in off-test.Laying hen feedstuff-egg ratio, death rate improvement situation see Table 3.
Table 3 laying hen feedstuff-egg ratio, death rate improve situation
By table 3 test-results as can be known, Clostridium butyricum HM-68 viable count reaches 1 * 10 in the feed
5Cfu/g, feedstuff-egg ratio and death rate significantly reduce behind the laying hen of feeding.
Claims (5)
1. a strain is used for the good clostridium butylicum that egg fowl is cultured, and it is characterized in that: described clostridium butylicum (Clostridium butyricum HM-68) separates from the healthy chicken enteron aisle and obtains; This bacterial strain has been preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, and preserving number: CGMCCNo.6732, preservation date are on October 29th, 2012.
2. the application of a clostridium butylicum is characterized in that: be used for improving egg fowl and culture.
3. the activation of a clostridium butylicum and cultivation, it is characterized in that: the Clostridiumbutyricum HM-68 of freezing preservation is inoculated in the GPY liquid nutrient medium, cultivate 20h down for 37 ℃ in temperature, so going down to posterity to cultivate obtains described activation Clostridium butyricum HM-68 bacterial classification 2-3 time; Described GPY liquid nutrient medium is composed as follows: peptone 10.0g, yeast extract paste 10.0g, glucose 6.0g, MgSO
47H
2O1.0g, K
2HPO
42.0g, MnSO
450ppm adds 1L distilled water, regulates pH to 6.8,121 ℃ of sterilization 15min.
4. the preparation of a clostridium butylicum bacterium powder is characterized in that the bacterium powder is made in bacterial classification inoculation fermentation, drying, the pulverizing of activation, makes its viable count reach 1 * 10
9Cfu/g.
5. the using method of a clostridium butylicum in feed is characterized in that: the bacterium powder after strain fermentation, freeze-drying, the pulverizing and basal diet are stirred stand-by, the clostridium butyricum active bacteria number reaches 1 * 10 in the feed
5Cfu/g.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104277999A (en) * | 2014-09-15 | 2015-01-14 | 中国农业大学 | Novel multiple-effect Clostridium butyricum and application thereof in aspects of enhancing oxidation resistance of animals and improving meat quality |
| CN104366110A (en) * | 2014-11-24 | 2015-02-25 | 山东龙盛农牧集团有限公司 | Antibiotic-free full-value biological feed for laying hens and preparation method thereof |
| CN104397408A (en) * | 2014-11-24 | 2015-03-11 | 山东龙盛农牧集团有限公司 | Antibiotic-free concentrated feed for laying chicks and preparation method of antibiotic-free concentrated feed |
| CN104397502A (en) * | 2014-11-24 | 2015-03-11 | 山东龙盛农牧集团有限公司 | Biological antibiotic-free concentrated feed for laying hens and preparation method thereof |
| CN106479924A (en) * | 2016-10-31 | 2017-03-08 | 湖北绿雪生物科技有限公司 | The preparation of a kind of Clostridium butyricum and clostridium butyricum active bacteria preparation and application |
| CN107118997A (en) * | 2017-07-11 | 2017-09-01 | 福建贝迪药业有限公司 | A kind of feeding clostridium butyricum, the probiotics for promoting growth of animals or poultry and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1771831A (en) * | 2004-11-12 | 2006-05-17 | 浙江大舜恒益生物技术有限公司 | Feed-grade microbial additive and preparation and application thereof |
| CN102226158A (en) * | 2011-05-09 | 2011-10-26 | 北京农业生物技术研究中心 | Clostridium butyricum SZ11 and application thereof |
| CN102321552A (en) * | 2011-08-12 | 2012-01-18 | 北京金泰得生物科技股份有限公司 | Clostridium butyricum used for feeding, and application thereof |
-
2013
- 2013-03-15 CN CN201310083900.2A patent/CN103205375B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1771831A (en) * | 2004-11-12 | 2006-05-17 | 浙江大舜恒益生物技术有限公司 | Feed-grade microbial additive and preparation and application thereof |
| CN102226158A (en) * | 2011-05-09 | 2011-10-26 | 北京农业生物技术研究中心 | Clostridium butyricum SZ11 and application thereof |
| CN102321552A (en) * | 2011-08-12 | 2012-01-18 | 北京金泰得生物科技股份有限公司 | Clostridium butyricum used for feeding, and application thereof |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104277999A (en) * | 2014-09-15 | 2015-01-14 | 中国农业大学 | Novel multiple-effect Clostridium butyricum and application thereof in aspects of enhancing oxidation resistance of animals and improving meat quality |
| CN104277999B (en) * | 2014-09-15 | 2018-12-25 | 中国农业大学 | A kind of novel multi-effect clostridium butyricum and its application in terms of improving animal oxidation resistance and improving meat |
| CN104366110A (en) * | 2014-11-24 | 2015-02-25 | 山东龙盛农牧集团有限公司 | Antibiotic-free full-value biological feed for laying hens and preparation method thereof |
| CN104397408A (en) * | 2014-11-24 | 2015-03-11 | 山东龙盛农牧集团有限公司 | Antibiotic-free concentrated feed for laying chicks and preparation method of antibiotic-free concentrated feed |
| CN104397502A (en) * | 2014-11-24 | 2015-03-11 | 山东龙盛农牧集团有限公司 | Biological antibiotic-free concentrated feed for laying hens and preparation method thereof |
| CN106479924A (en) * | 2016-10-31 | 2017-03-08 | 湖北绿雪生物科技有限公司 | The preparation of a kind of Clostridium butyricum and clostridium butyricum active bacteria preparation and application |
| CN106479924B (en) * | 2016-10-31 | 2018-06-12 | 湖北绿雪生物科技有限公司 | The preparation and application of a kind of clostridium butyricum and clostridium butyricum active bacteria preparation |
| CN107118997A (en) * | 2017-07-11 | 2017-09-01 | 福建贝迪药业有限公司 | A kind of feeding clostridium butyricum, the probiotics for promoting growth of animals or poultry and preparation method thereof |
| CN107118997B (en) * | 2017-07-11 | 2019-11-15 | 福建贝迪药业有限公司 | A kind of feeding clostridium butyricum, the probiotics for promoting growth of animals or poultry and preparation method thereof |
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| CN103205375B (en) | 2015-04-08 |
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