CN103849569B - The cultural method of the marine microalgae of rich selenium and the application in feed immunopotentiator thereof - Google Patents
The cultural method of the marine microalgae of rich selenium and the application in feed immunopotentiator thereof Download PDFInfo
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Abstract
The invention discloses a kind of utilize under solid conditions to hold concurrently support mode large-scale production and be rich in micro-algae of immune active ingredient selenium and be applied to and in animal and fowl fodder, form a kind of new immunopotentiator. The present invention cultivates the marine microalgae of rich selenium taking agricultural byproducts wheat bran, dregs of beans as main solid medium, and rich selenium microalgae powder is crushed to 200 object fineness, press the rich selenium algae powder of 0.1-0.6wt% amount interpolation in animal and fowl fodder, feeds livestock and poultry. For the present invention, three-dimensional mode, solid medium are cultivated micro-algae on a large scale, can save space, improve the collection rate of micro-algae; Add rich selenium algae powder and make livestock and poultry weightening finish improve 6-8%, feed conversion rate improves 10%, and antibiotic use amount reduces 20%.
Description
Technical field
The invention belongs to the crossing domain of marine microalgae and livestock and poultry breeding industry, relate to one and utilize double supporting under solid conditionsMode large-scale production is rich in micro-algae of immune active ingredient selenium and is applied to and in animal and fowl fodder, forms a kind of new immunity and strengthenAgent.
Background technology
Fowl poultry immune reinforcing agent also claims immunologic adjuvant, is that a class improves body to antigen or micro-life by non-specific approachThe material of thing specific reaction, and can improve the immune response of livestock and poultry body. From nineteen twenty-five France immunologist animal doctor that holds concurrentlyGastonRamon finds to add some material having no truck with can strengthen specifically body dialogue larynx and broken wound in vaccineSince the opposing reaction of wind toxin, it is very important that the development of immunopotentiator has become in current biological products research oneField, wherein bioactie agent micro-, efficient, noresidue is of greatest concern.
At present conventional immunopotentiator has selenium, bacteria lipopolysaccharide, muramyl dipeptide, coriolan and lentinan etc.
In livestock and poultry animal cultivation, along with the development of intensive culture industry, animal disease is more and more higher, and people have toA large amount of antibiotic and chemotherapeutants of using, make the pathogen resistance to the action of a drug day by day serious, and threaten the mankind's health. ChinaIn animal and fowl fodder, add multiple antimicrobial, anthelmintic, hormone and somatotrophic medicine or preparation, these medicines and preparation all canCause residual in animal products, the health of the large multipair human body of these residues works the mischief. So, the peace of livestock productsFull property must be brought up to due attention degree. Find a kind of substitute, make it drug residue free and free from environmental pollution, Cheng LiaoThe task of top priority of Modern Animal Husbandry. Its application can reduce the medicament residue and the environmental pollution that cause because of various medicines, instituteWith the Application and Development of novel fowl poultry immune reinforcing agent, solving the control of livestock and poultry pestilence, security and the environment dirt of livestock productsDye the aspects such as problem and play a positive role, can also improve livestock products quality, increase considerably added value of product. Therefore, grindOne processed has naturality, pollution-free, no drug residue, without the resistance to the action of a drug, have no side effect and be beneficial to the features such as environmental protection and advantageFeeding immunopotentiator become recent tendency.
In livestock and poultry disease clinically, comparison extensive use is the high Xue of the exempting from Qing of specificity ﹑ Gan Rao Su ﹑ thymic peptide and transfer factorIn the goods that strengthen immunologic function, but because these medicine major parts have strict species specificity, current animal drugs in additionProduct market is more chaotic, and price is high, difficult quality guarantee, and in clinical practice, be subject to the source of products material and costRestriction. Therefore must select a kind of safe and reliablely, inexpensive efficient, there is again the immunopotentiator of popularity immunological enhancement.So plant source and microorganism immunopotentiator have development potentiality most, Ye Shi China researchs and solves antibiotic residue problemKey subject is the effective way of breaking through trade barrier. Along with the raising of the sanitary standard of China's meat product, feed safetyBe the primary condition that ensures animal based food safety, development pollution-free green food, is also the livestock and poultry cultivation task of top priority. ForOpen up the source of feed addictive, guarantee the safety of animal products, many scholars also forward natural plant to sightWith next on additive for microbe feedstuff, the mode of action of their uniquenesses, good action effect and noresidue, nonreactive medicineProperty, the feature such as pollution-free are subject to increasing favor.
Micro-algae is on the earth, to occur biology the earliest, and their individualities small (several microns to tens microns) generally will be aobviousUnder micro mirror, just can see. Main primary producer in micro-algae ecosystem, kind is many, and breeding is fast, at the material of the ecosystemIn circulation and energy flow, play an important role. In recent decades, along with the application of modern biotechnology, separate qualificationThe raising of means, the fast development of genetic engineering, genetic engineering etc., the mankind to the research and development of micro-algae entered one brand-newPeriod. Because micro-algae is nutritious, be rich in trace element and different kind organism active material, and be easy to artificial propagation, growth speedDegree is fast, and the breeding cycle is short, so in medicine, health products, cosmetics, aquaculture bait, feed addictive, chemical industry and environmental protection etc.Aspect has broad application prospects.
That selenium has is anti-ageing, anti-oxidant, strengthen the so many bioactive functions such as immunity, but inorganic selenium has poisonProperty, its using dosage and scope are all subject to strict restriction. Therefore, be only converted into Organic Selenium, just have edible, feeding and protectStrong value. Organic Selenium adopts animals and plants or microbial transformation mode to obtain mostly at present. Research shows, selenium can participate in algaeThe metabolic process of cell, and by the bioconcentration of algae and along food chain transmission, marine animal and even the mankind are producedImportant impact. Selenium in frond can with fat, polysaccharide and protein combination, be that it is converted into the main of Organic Selenium but form selenoproteinMechanism, marine microalgae contains abundant nutrition and bioactivator mostly, and many trace elements and compound are all hadStronger enrichment is selectively good, efficient, biological concentration body that cost is low, easy and simple to handle of a class.
The invention solves the micro-algae technology of extensive solid-state cultivation, can promote micro-algae industrialization level, can also leadLivestock and poultry cultivation industry is to green, healthy, secure side to striding forward, for China's poultry and livestock provides safety guarantee, in the future IState's development green agriculture, stockbreeding industry are established strong basis.
Summary of the invention
The present invention has following two objects:
1. adopt stereoscopic culture mode, taking agricultural byproducts wheat bran, dregs of beans as ocean that main solid medium is cultivated rich seleniumMicro-algae.
2. rich selenium marine microalgae is applied to livestock and poultry cultivation. The object of the invention 1 is achieved in that a kind of sea of rich seleniumThe cultural method of the micro-algae in ocean, comprises the following steps:
1, configure solid medium: wheat bran 65-88wt%, dregs of beans 8-33wt%, lime 1-2wt%, KH2PO41-2wt%, addsEnter rich selenium powder 0.02-0.1wt%, every 100 kilograms of the formula of aforementioned proportion, adding salinity is the underground sea water 50-of 30-35wt ‰83 kilograms stir, and are placed in the interior boiling of autoclave 120 minutes, and 125 DEG C of holding temperatures, air pressure 0.01-0.02MPa, by boilingGood above-mentioned culture medium is cooled to 23 DEG C, stand-by;
2, inoculation: in aseptic room, the algae kind of marine microalgae is spilled in above-mentioned culture medium, has inoculated and stirred allEven;
3, stereoscopic culture: culture medium is put into the transparent tray of plastic-steel, and the hole of the full diameter 3mm of tray base fabric, is placed in asepticCultivate in room, pass into pure air, throughput is 1m3/ min, 21 DEG C-26 DEG C of temperature, illuminance 1000-3000lx, cultivates42-72 hour;
4, desiccation culture completes, and by cultured material, puts into boiling drier dry, controls dryer entrance mouth temperatureAt 50-65 DEG C, 35 DEG C-45 DEG C of air outlets, the dry rear moisture that keeps material is controlled in 10%.
Underground sea water described in step 1 filters with double-deck 200 order cottons.
The algae kind of the marine microalgae described in step 2 is prepared and is passed through following steps: marine chlorella is cultured to logarithmic phaseAfter move to 3L triangular flask by 250mL triangular flask and expand cultivation, inoculum density is 40mL/L, throughput is 2.0L/min, temperatureDegree is controlled at 23 DEG C ± 2 DEG C, and intensity of illumination is 3000lx, Light To Dark Ratio 12h:12h, and culture medium is f/2, timing shake training every daySupport bottle 3 times, cultivate 2d.
The specification of the transparent tray of described plastic-steel described in step 3 is 64CM × 45CM × 4.5CM.
Pure air described in step 3 refers to the air of crossing filtration through the aperture of 0.01 μ m.
The application of the marine microalgae that the object of the invention 2 is achieved in that a kind of rich selenium in feed immunopotentiator,Comprise the steps:
Above-mentioned rich selenium microalgae powder is crushed to 200 object fineness, in animal and fowl fodder, presses 0.1-0.6wt% amount and add rich seleniumAlgae powder, feeds livestock and poultry.
The present invention compared with prior art, its remarkable advantage:
1, cultivate marine microalgae taking wheat bran and dregs of beans as primary raw material, cost reduces greatly, and raw material sources are easy;
2, cultivate on a large scale micro-algae with three-dimensional mode, solid medium, can save space, improve the collection of micro-algaeRate;
3, add rich selenium algae powder and make livestock and poultry weightening finish improve 6-8%, feed conversion rate improves 10%, and antibiotic use amount reduces20%。
Brief description of the drawings
Fig. 1 is the preparation flow schematic diagram of rich selenium microalgae powder of the present invention.
Detailed description of the invention
The preparation flow of following embodiment is shown in Fig. 1.
Embodiment mono-
1, algae kind is prepared: marine chlorella is cultured to and moves to 3L triangular flask by 250mL triangular flask after logarithmic phase and expandLarge cultivation, inoculum density is 40mL/L, and throughput is 2.0L/min, and temperature is controlled at 23 DEG C ± 2 DEG C, and intensity of illumination is3000lx, Light To Dark Ratio 12h:12h, culture medium is f/2, every day, timing was shaken blake bottle 3 times, cultivated 2d.
2, configure solid medium: by wheat bran 65wt%, dregs of beans 33wt%, lime 1wt% and KH2PO41wt% mixes,Add rich selenium powder 0.02wt%, every 100 kilograms of the formula of aforementioned proportion, adds by the salinity that double-deck 200 order cottons are crossed filtration to be83 kilograms of the underground sea waters of 35wt ‰ stir, and pack food steamer into and put into autoclave boiling 120 minutes, 125 DEG C of holding temperatures,Air pressure 0.01-0.02MPa, is cooled to 23 DEG C by well-done above-mentioned culture medium, stand-by;
3, inoculation: in aseptic room, the algae kind of marine microalgae is spilled in above-mentioned culture medium, has inoculated and stirred allEven;
4, stereoscopic culture: culture medium is put into the transparent tray of plastic-steel, and the specification of tray is 64CM × 45CM × 4.5CM,The hole of the full diameter 3mm of tray base fabric, is placed in sterile culture room, passes into the pure air of crossing filtration through the aperture of 0.01 μ m,Throughput is 1m3/ min, 26 DEG C of temperature, illuminance 2000lx, cultivates 42 hours;
5, desiccation culture completes, and by cultured material, puts into boiling drier dry, controls dryer entrance mouth temperatureAt 50 DEG C, 35 DEG C of air outlets, the dry rear moisture that keeps material is controlled in 10%.
Experimental animal provides with 20 of 25 age in days weanling pigs, pig farm, Chang Mao big restaurant, and the drinking-water of sucking the breast is normal, and body surface is strongHealth. By being selected from 20 of the weanling pigs that 7 nest body weight are suitable, be divided at random two groups of A, B, 10 every group, wherein, A group and BGroup feeding feed stuff formula is identical: corn 63%, dregs of beans 22%, wheat bran 7%, fish meal 6%, zeolite powder 2%, add respectively 0.1 in A groupThe rich selenium algae powder of wt%, 0.3wt% and 0.6wt%.
B group is not added, A, two groups of equal Escherichia Coli Injection bacterium liquid (1 × 10 of B8Cfu/mL), after infection respectively at 1,2,3,4,5 day stipulated time extracted porcine vein liquid 1mL with asepsis injector, was placed in 1.5mL centrifuge tube, added anticoagulant heparin agent mixedEven, after 4 DEG C of Refrigerator store 24h, 5000rpm, 4 DEG C of centrifugal 10min, abandon precipitation, gets upper serum-20 DEG C and save backup.
The diet of test group and control group, except in premix, added " rich selenium algae powder compound " and do not add different itOutward, basestocks used is all identical. Raise in nursing process, adopt the funneling poor free choice feeding of raising, in liberal supply drinking-water, daily pipeReason is identical with production.
The mensuration of immune indexes, the determination of activity of AKP, ACP, SOD and CAT all adopts Nanjing, Jiangsu Province to build up biologyKit and assay method thereof that research institute produces, experiment confirmation, A group AKP, ACP activity reach the highest for the 3rd day after injection,SOD the 4th day is the highest, and CAT inject within latter second day, just reach the highest, the highest group with B group be more all significant difference (p <0.05), ACP, CAT peak are 3 times more than of control group B group, illustrating that the rich selenium algae powder of the interpolation 0.1-0.6wt% that feeds can haveEffect activates the immunocompetence of pig, improves the resistivity of pig to microorganism.
Examine and record the ingesting of pig, drinking-water and the state of mind, and weigh by head in 40 age in days 70 ages in days, experimentThe statistical analysis A group of data system exceeds 0.64Kg than B group in 40 age in days average weights, on average exceeds nearly 2Kg when 70 age in days, saysBright rich selenium algae powder has certain short long-acting fruit.
Embodiment bis-
1, algae kind is prepared: marine chlorella is cultured to and moves to 3L triangular flask by 250mL triangular flask after logarithmic phase and expandLarge cultivation, inoculum density is 40mL/L, and throughput is 2.0L/min, and temperature is controlled at 23 DEG C ± 2 DEG C, and intensity of illumination is3000lx, Light To Dark Ratio 12h:12h, culture medium is f/2, every day, timing was shaken blake bottle 3 times, cultivated 2d.
2, configure solid medium: by wheat bran 88wt%, dregs of beans 8wt%, lime 2wt% and KH2PO42wt% mixes, and addsEvery 100 kilograms of the formula of rich selenium powder 0.05wt%, aforementioned proportion, add with the salinity that double-deck 200 order cottons are crossed filtration be 34wt ‰75 kilograms of underground sea waters stir, pack food steamer into and put into autoclave boiling 120 minutes, 125 DEG C of holding temperatures, air pressure0.01-0.02MPa, is cooled to 23 DEG C by well-done above-mentioned culture medium, stand-by;
3, inoculation: in aseptic room, the algae kind of marine microalgae is spilled in above-mentioned culture medium, has inoculated and stirred allEven;
4, stereoscopic culture: culture medium is put into the transparent tray of plastic-steel, and the specification of tray is 64CM × 45CM × 4.5CM,The hole of the full diameter 3mm of tray base fabric, is placed in sterile culture room, passes into the pure air of crossing filtration through the aperture of 0.01 μ m,Throughput is 1m3/ min, 25 DEG C of temperature, illuminance 2200lx, cultivates 60 hours;
5, desiccation culture completes, and by cultured material, puts into boiling drier dry, controls dryer entrance mouth temperatureAt 55 DEG C, 40 DEG C of air outlets, the dry rear moisture that keeps material is controlled in 10%.
Experimental animal provides with 20 of 25 age in days weanling pigs, pig farm, Chang Mao big restaurant, and the drinking-water of sucking the breast is normal, and body surface is strongHealth. By being selected from 20 of the weanling pigs that 7 nest body weight are suitable, be divided at random two groups of A, B, 10 every group, wherein, A group and BGroup feeding feed stuff formula is identical: corn 63%, dregs of beans 22%, wheat bran 7%, fish meal 6%, zeolite powder 2%, add respectively 0.1 in A groupThe rich selenium algae powder of wt%, 0.3wt% and 0.6wt%.
B group is not added, A, two groups of equal Escherichia Coli Injection bacterium liquid (1 × 10 of B8Cfu/mL), after infection respectively at 1,2,3,4,5 day stipulated time extracted porcine vein liquid 1mL with asepsis injector, was placed in 1.5mL centrifuge tube, added anticoagulant heparin agent mixedEven, after 4 DEG C of Refrigerator store 24h, 5000rpm, 4 DEG C of centrifugal 10min, abandon precipitation, gets upper serum-20 DEG C and save backup.
The diet of test group and control group, except in premix, added " rich selenium algae powder compound " and do not add different itOutward, basestocks used is all identical. Raise in nursing process, adopt the funneling poor free choice feeding of raising, in liberal supply drinking-water, daily pipeReason is identical with production.
The mensuration of immune indexes, the determination of activity of AKP, ACP, SOD and CAT all adopts Nanjing, Jiangsu Province to build up biologyKit and assay method thereof that research institute produces, experiment confirmation, A group AKP, ACP activity reach the highest for the 3rd day after injection,SOD the 4th day is the highest, and CAT inject within latter second day, just reach the highest, the highest group with B group be more all significant difference (p <0.05), ACP, CAT peak are 3 times more than of control group B group, illustrating that the rich selenium algae powder of the interpolation 0.1-0.6wt% that feeds can haveEffect activates the immunocompetence of pig, improves the resistivity of pig to microorganism.
Examine and record the ingesting of pig, drinking-water and the state of mind, and weigh by head in 40 age in days 70 ages in days, experimentThe statistical analysis A group of data system exceeds 0.71Kg than B group in 40 age in days average weights, on average exceeds nearly 2.5Kg when 70 age in days,Illustrate that rich selenium algae powder has certain short long-acting fruit.
Embodiment tri-
1, algae kind is prepared: marine chlorella is cultured to and moves to 3L triangular flask by 250mL triangular flask after logarithmic phase and expandLarge cultivation, inoculum density is 40mL/L, and throughput is 2.0L/min, and temperature is controlled at 23 DEG C ± 2 DEG C, and intensity of illumination is3000lx, Light To Dark Ratio 12h:12h, culture medium is f/2, every day, timing was shaken blake bottle 3 times, cultivated 2d.
2, configure solid medium: by wheat bran 82wt%, dregs of beans 15wt%, lime 1.5wt%KH2PO41.5wt% mixing,Add rich selenium powder 0.1wt%, every 100 kilograms of the formula of aforementioned proportion, adds by the salinity that double-deck 200 order cottons are crossed filtration to be50 kilograms of the underground sea waters of 30wt ‰ stir, and pack food steamer into and put into autoclave boiling 120 minutes, 125 DEG C of holding temperatures,Air pressure 0.01-0.02MPa, is cooled to 23 DEG C by well-done above-mentioned culture medium, stand-by;
3, inoculation: in aseptic room, the algae kind of marine microalgae is spilled in above-mentioned culture medium, has inoculated and stirred allEven;
4, stereoscopic culture: culture medium is put into the transparent tray of plastic-steel, and the specification of tray is 64CM × 45CM × 4.5CM,The hole of the full diameter 3mm of tray base fabric, is placed in sterile culture room, passes into the pure air of crossing filtration through the aperture of 0.01 μ m,Throughput is 1m3/ min, 21 DEG C of temperature, illuminance 3000lx, cultivates 72 hours;
5, desiccation culture completes, and by cultured material, puts into boiling drier dry, controls dryer entrance mouth temperatureAt 50 DEG C, 35 DEG C of air outlets, the dry rear moisture that keeps material is controlled in 10%.
Experimental animal provides with 20 of 25 age in days weanling pigs, pig farm, Chang Mao big restaurant, and the drinking-water of sucking the breast is normal, and body surface is strongHealth. By being selected from 20 of the weanling pigs that 7 nest body weight are suitable, be divided at random two groups of A, B, 10 every group, wherein, A group and BGroup feeding feed stuff formula is identical: corn 63%, dregs of beans 22%, wheat bran 7%, fish meal 6%, zeolite powder 2%, add respectively 0.6wt% in A groupRich selenium algae powder.
B group is not added, A, two groups of equal Escherichia Coli Injection bacterium liquid (1 × 10 of B8Cfu/mL), after infection respectively at 1,2,3,4,5 day stipulated time extracted porcine vein liquid 1mL with asepsis injector, was placed in 1.5mL centrifuge tube, added anticoagulant heparin agent mixedEven, after 4 DEG C of Refrigerator store 24h, 5000rpm, 4 DEG C of centrifugal 10min, abandon precipitation, gets upper serum-20 DEG C and save backup.
The diet of test group and control group, except in premix, added " rich selenium algae powder compound " and do not add different itOutward, basestocks used is all identical. Raise in nursing process, adopt the funneling poor free choice feeding of raising, in liberal supply drinking-water, daily pipeReason is identical with production.
The mensuration of immune indexes, the determination of activity of AKP, ACP, SOD and CAT all adopts Nanjing, Jiangsu Province to build up biologyKit and assay method thereof that research institute produces, experiment confirmation, A group AKP, ACP activity reach the highest for the 3rd day after injection,SOD the 4th day is the highest, and CAT inject within latter second day, just reach the highest, the highest group with B group be more all significant difference (p <0.05), ACP, CAT peak are 3 times more than of control group B group, illustrate feeds add 0.1-0.6wt% richness selenium algae powder can be effectiveActivate the immunocompetence of pig, improve the resistivity of pig to microorganism.
Examine and record the ingesting of pig, drinking-water and the state of mind, and weigh by head in 40 age in days 70 ages in days, experimentThe statistical analysis A group of data system exceeds 0.78Kg than B group in 40 age in days average weights, on average exceeds nearly 3Kg when 70 age in days, saysBright rich selenium algae powder has certain short long-acting fruit.
Effectiveness forecasting of the present invention, adds the rich selenium algae of 0.1-0.6wt% powder and makes livestock and poultry weightening finish improve 6-8% in feed, feed turnsRate improves 10-12%, and antibiotic use amount reduces 20-23%.
Claims (6)
1. a cultural method for the marine microalgae of rich selenium, is characterized in that comprising the following steps:
1, configure solid medium: wheat bran 65-88wt%, dregs of beans 8-33wt%, lime 1-2wt%, KH2PO41-2wt%, addsEnter rich selenium powder 0.02-0.1wt%, every 100 kilograms of the formula of aforementioned proportion, adding salinity is the underground sea water 50-of 30-35wt ‰83 kilograms stir, and are placed in the interior boiling of autoclave 120 minutes, and 125 DEG C of holding temperatures, air pressure 0.01-0.02MPa, by boilingGood above-mentioned culture medium is cooled to 23 DEG C, stand-by;
2, inoculation: in aseptic room, the algae kind of marine microalgae is spilled in above-mentioned culture medium, has inoculated and stirred;
3, stereoscopic culture: culture medium is put into the transparent tray of plastic-steel, and the hole of the full diameter 3mm of tray base fabric, is placed in sterile cultureFang Zhong, passes into pure air, and throughput is 1m3/ min, 21 DEG C-26 DEG C of temperature, illuminance 1000-3000lx, cultivates 42-72 littleTime;
4, desiccation culture completes, and by cultured material, puts into boiling drier dry, controls dryer entrance mouth temperature and exists50-65 DEG C, 35 DEG C-45 DEG C of air outlets, the dry rear moisture that keeps material is controlled in 10%.
2. the cultural method of the marine microalgae of rich selenium according to claim 1, is characterized in that underground described in step 1Seawater filters with double-deck 200 order cottons.
3. the cultural method of the marine microalgae of rich selenium according to claim 1, is characterized in that the ocean described in step 2The algae kind of micro-algae is prepared and is passed through following steps: after marine chlorella is cultured to logarithmic phase, move to 3L triangle by 250mL triangular flaskBottle expands cultivation, and inoculum density is 40mL/L, and throughput is 2.0L/min, and temperature is controlled at 23 DEG C ± 2 DEG C, intensity of illuminationFor 3000lx, Light To Dark Ratio 12h:12h, culture medium is f/2, every day, timing was shaken blake bottle 3 times, cultivated 2d.
4. the cultural method of the marine microalgae of rich selenium according to claim 1, is characterized in that the plastic-steel described in step 3The specification of transparent tray is 64CM × 45CM × 4.5CM.
5. the cultural method of the marine microalgae of rich selenium according to claim 1, is characterized in that the cleaning described in step 3Air refers to the air of crossing filtration through the aperture of 0.01 μ m.
6. the application of the rich selenium marine microalgae that according to claim 1 prepared by method in feed immunopotentiator, its featureBe rich selenium microalgae powder to be crushed to 200 object fineness, in animal and fowl fodder, press the amount of 0.1-0.6wt% and add, feed poultryFowl.
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| CN105110913A (en) * | 2015-08-12 | 2015-12-02 | 南京龙源生态农业有限公司 | Dendrobium candidum multifunctional fertilizer and preparation method thereof |
| CN105707446A (en) * | 2016-02-01 | 2016-06-29 | 周霞轩 | Pure natural selenium-enriched chicken premix feed preparation method |
| CN106520556A (en) * | 2016-09-23 | 2017-03-22 | 东台市赐百年生物工程有限公司 | Method for large scale culture of nannochloropsis oculata |
| CN106635919A (en) * | 2017-01-16 | 2017-05-10 | 江西中藻生物科技股份有限公司 | Spirulina culture method |
| CN107988121B (en) * | 2018-01-13 | 2021-01-29 | 杭州富阳优信科技有限公司 | Process for culturing spirulina platensis |
| CN110317769B (en) * | 2019-07-18 | 2021-04-20 | 恩施硒圣植物科技有限公司 | Culture method of selenium-rich microalgae |
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| CN1085951A (en) * | 1992-10-16 | 1994-04-27 | 中国农业科学院土壤肥料研究所 | The spirulina technique of enriched selenium |
| CN101715986A (en) * | 2009-11-20 | 2010-06-02 | 深圳大学 | Preparation method and application of rich selenium haematococcus powder |
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| JP2000224981A (en) * | 1999-02-04 | 2000-08-15 | Marine Biotechnol Inst Co Ltd | Medium for marine microalgae and culture of marine microalgae using the same |
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| CN1085951A (en) * | 1992-10-16 | 1994-04-27 | 中国农业科学院土壤肥料研究所 | The spirulina technique of enriched selenium |
| CN101715986A (en) * | 2009-11-20 | 2010-06-02 | 深圳大学 | Preparation method and application of rich selenium haematococcus powder |
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