CN103205318A - Laundry detergent - Google Patents

Abstract

The invention discloses a laundry detergent, which belongs to the field of hygiene products. The invention aims to provide a highly efficient and safe product which is processed by using modern biotechnology. The laundry detergent provided by the invention has a function of removing dirt on clothes and the advantages of remarkable washing ability, strong detergency and convenient usage; in particular, the laundry detergent has good functions of sterilization and disinfection, especially the characteristic of capability of killing methicillin-resistant staphylococcus aureus. Cheap fermentation raw materials and modern biotechnology are utilized for production of a bactericide added in the laundry detergent, so the advantages of a simple process and low cost are obtained. Therefore, the laundry detergent provided by the invention has strong bactericidal activity, is safe and cheap and has high technical content.

Description

A kind of washing liquid

Technical field: the invention belongs to field of hygiene products, relate to special washing liquid.

Background technology:

On the market, some have also occurred and had the washing liquid of disinfecting and sterilizing functions.Mainly be to have added some sterilizing agents and Chinese medicinal materials etc., make washing powder have certain germicidal efficacy.These sterilizing agents, some is not really safe to people, if rinsing is unclean, people's skin is had certain stimulation.Some sterilizing agent, expensive, be unfavorable for reducing production costs and increasing competitiveness of product.At present, it is strong to be starved of a kind of soil removability on the market, and sterilizing power is strong, safety, cheap washing liquid.

Summary of the invention:

The purpose of this invention is to provide a kind of highly effective and safe, adopt the washing liquid product of modern biotechnology product processing.

Prescription of the present invention mainly contains: sterilant, anion surfactant, nonionogenic tenside, amphoterics, whitening agent, Chinese honey locust distillate, essence, deionized water.

Major technique feature of the present invention be added have inexpensive, highly effective and safe, the sterilant through modern biotechnology processing of All Pure Nature.Sterilant is subtilis (Bacillus subtilis) fermentation broth extract, this bacterial strain has been preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on April 16th, 2012, the preservation address is positioned at the Yard 1, BeiChen xi Road, Chaoyang District, Beijing City No. 3, (postcode 100101), deposit number are CGMCCNo.6012.

Fungus characteristic is as follows:

1. somatic cells is life or the living Gram-positive of near-end in direct rod shape, the gemma.

2. colony characteristics is that white is circular, protuberance, and smooth surface, opaque, moistening, provoke adhesion.

3. can utilize various saccharides, as glucose, semi-lactosi, wood sugar, cellobiose, raffinose etc.; Produce amylase, proteolytic enzyme, can gelatin hydrolysate, V.P, the methyl red positive.

4. the suitableeest culture temperature is 37 ℃, optimal pH 7.0.

This bacterium classification status is as follows: bacterium territory (Bacteria); Firmicutes (Firmicutes); Genus bacillus guiding principle (Bacillales); Genus bacillus order (Bacillaceae); Bacillaceae (Bacillus); Bacillus (Bacillus); Bacillus subtilis bacterial classification (Bacillus subtilis).

The sterilant addition is product weight (0.1 ~ 1%).

The preparation method of fermentation broth extract is as follows among the present invention:

The seed culture medium of bacterial strain is broth culture, extractum carnis 2g wherein, peptone 8g, NaCl5g, water 1000ml, pH value 7.0 ~ 7.2, high pressure steam sterilization.

The seed liquor culture condition: culture temperature is 33 ℃, and shaking speed is 140 rev/mins, and fermentation time is 13 hours.

Inoculum size is 8%, is conducive to the formation of antibacterial substance.

The fermentation fermention medium consists of: glucose 0.3%, and peptone 1%, NaCl 0.5%, MgSO ₄.7H ₂O 0.7%, KH ₂PO ₄0.7%, pH8.0.

Fermentation culture conditions is: culture temperature is 33 ℃, and shaking speed is 140 rev/mins, and fermentation time is about 24 hours.

Extracting the antibacterial substance method from fermented liquid is organic solvent precipitation method.

At first with the fermented liquid high speed centrifugation, temperature 0-4 ℃, rotating speed is 8000 rev/mins, centrifugal ten minutes, keeps supernatant liquor.

(methyl alcohol: ethanol=1:6), fully dissolving is placed in the there-necked flask, and 40 degrees centigrade of temperature are controlled in water-bath, and stirring velocity is 60rpm, and the time is 5 hours, collects organic phase, adopts vacuum distillation method to remove organic solvent to add the equal-volume organic solvent in the supernatant liquor.Distillation temperature 30-60 ℃, distilled 20 minutes, organic solvent is removed.The collecting precipitation material.Namely get sterilant.

The crude extract of bacteriocidal substance, after water dissolution, concentration is the 1%(mass volume ratio).Lysate can kill common pathogenic bacterium effectively.For example not only can kill intestinal bacteria effectively, Salmonellas, the pathogenic micro-organism that golden yellow common coccus etc. are common, and can kill the common coccus of methicillin-resistant staphylococcus.

This disinfecting liquid has high security, in the emergency toxicology experiment, we gavage 5 groups of 200 mouse with this disinfecting liquid, and the mouse of experimental group none occur dead, and subtilis is present in occurring in nature widely, and many important industrial bacterial strains are exactly subtilis.Substantially can conclude that this disinfecting liquid is safe for the user, can relievedly use.After adding it to washing liquid, find that it still has good fungicidal activity.

Method for making of the present invention is simple, add and at first deionized water (20 ~ 45%) is added in the reactor man-hour, its temperature is heated to 70 ℃, add anion surfactant (10 ~ 15%) then successively, nonionogenic tenside (20 ~ 30%), amphoterics (8 ~ 15%) is after whitening agent (0.2 ~ 0.5%) stirs, cool the temperature to 40 ℃, with citric acid the pH value of solution is transferred to 6.5 ~ 7.0, add Chinese honey locust distillate (10 ~ 20%), sterilant (0.1 ~ 1%), essence, stir, mixing speed can be controlled in 20 ~ 30 rev/mins, and the feed liquid that stirs is outgased, adopt vacuum outgas, negative pressure can be controlled in 5Mpa, removes the bubble that forms in the stirring and dissolving process, reduces the air content of material, mixed solution is carried out packing, can obtain washing liquid.Ratio is the weight percent of each ingredients constitute product gross weight herein.

Anion surfactant is sodium lauryl sulphate, and is at least a in the fat alcohol polyethylene ether sodium sulfate.

Described nonionogenic tenside can be at least a in alkylphenol polyoxyethylene, the fatty alcohol-polyoxyethylene ether.

Amphoterics is at least a in Varion CDG-K or the dodecyl dimethyl ammonium oxide.

The Chinese honey locust distillate, with an amount of Chinese honey locust, add 4 times of water loggings bubble after, distill, obtain the distillate suitable with the Chinese honey locust quality.

Whitening agent is sodium ethylene diamine tetracetate.

Used essence can add according to the odor type of product, makes product have different odor types, satisfies different human consumers' needs.

Beneficial effect:

The present invention realized that not only washing liquid removes the function of dirt on the clothing, and washability is outstanding, strong detergency, easy to use, especially possessed the function of good sterilization and sterilization, particularly can kill the characteristic of methicillin-resistant staphylococcus aureus.The emergency toxicology experiment has also verified the sterilant that adds in this washing liquid people and animals to be had very high security.

That adds among the present invention has the rot-resistant function by the pure natural fermentation raw material through the sterilant that modern biotechnology processes, and has characteristics safely and efficiently.Need not to add again sanitas in the liquid detergent.

Specific embodiment:

Induction mutation of bacterium

Take the ultraviolet mutagenesis method: be starting strain with CICC10023, make bacteria suspension, coat on the substratum plate, cultivate in 37 ℃ of incubators behind the uv irradiating 90s.

Bacterial screening

Choose 30 single bacterium colonies of looking strong from the 90s plate, be inoculated on the inclined-plane, in 37 ℃ of incubators, cultivated 0--4 ℃ of stored refrigerated 24 hours; With original strain in contrast, adopt conventional seed selection condition, Xylo-Mucine is as carbon source and proteolysis circle method screening cellulase-producing and the strong bacterial strain of proteolytic enzyme, screening obtains cellulase-producing and strong bacterial strain subtilis (Bacillus subtilis) L1 of proteolytic enzyme, this bacterial strain cellulase-producing can improve 25% by the force rate original strain, produces proteolytic enzyme and can improve 52% by the force rate original strain.In the common micro-organisms center preservation of Chinese common micro-organisms culture presevation management committee, deposit number is CGMCC No6012 with this bacterial classification.

The used subtilis of this product (Bacillus subtilis) bacterial classification, this bacterial strain has been preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on April 16th, 2012, the preservation address is positioned at the Yard 1, BeiChen xi Road, Chaoyang District, Beijing City No. 3, (postcode 100101), deposit number are CGMCC No6012.

Studies show that by experiment: sterilant have good inhibition intestinal bacteria, streptococcus aureus, etc. the characteristics of pathogenic bacterium.

The invention will be further described below in conjunction with specific embodiment, but be not limited to embodiment.

100 kilograms of products of embodiment 1 preparation: the 45kg deionized water is added in the reactor, its temperature is heated to 70 ℃, add 10kg fat alcohol polyethylene ether sodium sulfate then successively, the 20kg fatty alcohol-polyoxyethylene ether, the 10kg Varion CDG-K, 0.3kg after sodium ethylene diamine tetracetate stirs, cool the temperature to 40 ℃, with citric acid the pH value of solution transferred to 6.5 ~ 7.0, add 14kg Chinese honey locust distillate, 0.2kg sterilant, the 0.0001kg rose compound stirs, mixing speed can be controlled in 20 ~ 30 rev/mins, the feed liquid that stirs is outgased, adopt vacuum outgas, negative pressure can be controlled in 5Mpa, the bubble that removal forms in the stirring and dissolving process, reduce the air content of material, mixed solution is carried out packing, can obtain washing liquid.

Get 9% concentration product of the present invention, adopt conventional microorganism detection sterilization effect, test-results shows that product sterilization effect of the present invention reaches more than 90%, and sterilization effect is obvious.Microorganism is selected intestinal bacteria, Salmonellas, golden yellow common coccus.

Claims (7)

1. a washing liquid comprises anion surfactant, nonionogenic tenside, and amphoterics, whitening agent, the Chinese honey locust distillate is characterized in that containing sterilant, and described sterilant is subtilis CGMCC6012 fermentation broth extract.

2. according to the described washing liquid of claim 1, the weight percent that it is characterized in that described sterilant is 0.1 ~ 1%.

3. according to the described washing liquid preparation method of claim 1, its preparation method is as follows: deionized water adds in the reactor, is heated to 70 ℃, the adding weight percent is 10 ~ 15% anion surfactant, 20 ~ 30% nonionogenic tenside, 8 ~ 15% amphoterics, 0.2 ~ 0.5% whitening agent stirs, and is cooled to 40 ℃, pH value transfers to 6.5 ~ 7.0, the Chinese honey locust distillate of adding 10 ~ 20%, 0.1 ~ 1% sterilant and essence stir, the degassing, packing.

4. according to the described washing liquid preparation method of claim 3, it is characterized in that described anion surfactant is sodium lauryl sulphate, fat alcohol polyethylene ether sodium sulfate or both mixtures.

5. according to the described washing liquid preparation method of claim 3, it is characterized in that described nonionogenic tenside is at least a in alkylphenol polyoxyethylene, the fatty alcohol-polyoxyethylene ether.

6. according to the described washing liquid preparation method of claim 3, it is characterized in that described amphoterics is at least a in Varion CDG-K or the dodecyl dimethyl ammonium oxide.

7. according to the described washing liquid preparation method of claim 3, it is characterized in that described whitening agent is sodium ethylene diamine tetracetate.