CN103142455A - Hand sanitizer - Google Patents

Abstract

The invention belongs to the field of hygiene products, and aims to provide a safe and efficient antibacterial hand sanitizer product processed by modern biotechnology. The hand sanitizer provided by the invention comprises a surface active agent, glycerol, an auxiliary agent and deionized water; and the hand sanitizer further comprises 0.1-1 wt% of a bactericide of an extract from Bacillus subtilis fermentation broth. A preparation method of the hand sanitizer comprises the following steps: adding deionized water into a reaction kettle, heating to 65 DEG C, adding glycerol, the surface active agent, stirring uniformly, cooling to 40 DEG C, adjusting a pH value to 6.5-7, adding the bactericide, a perfume and the auxiliary agent, stirring, degassing and packaging. The hand sanitizer provided by the invention realizes dirt removing ability, good sterilization and disinfection function, and high safety. The bactericide added in the invention has antiseptic function, and is safe and efficient; therefore, the hand sanitizer does not the need to be added with preservatives.

Description

A kind of handwashing liquid

Technical field

The invention belongs to field of hygiene products, relate to a kind of special handwashing liquid.

Background technology

Wanting to protect from infection, is at first the article of avoiding contact stain to cross, but in our Working Life, some article can't be avoided contacting.So we must form the custom of often washing one's hands.The articles for washing of selling in the market is mainly take decontamination as main, and major part does not have the function of sterilization.

Also occur some on market and had the handwashing liquid of disinfecting and sterilizing functions.Be mainly to have added some disinfectant, make handwashing liquid have certain bactericidal effect.These disinfectant, some is not bery safe to people, if rinsing is unclean, Person's skin is had certain stimulation.Some disinfectant, expensive, be unfavorable for reducing production costs and increase the competitiveness of product.At present, be starved of a kind of dirt-removing power on market strong, sterilizing power is strong, safety, cheap handwashing liquid.

Summary of the invention

The purpose of this invention is to provide a kind of highly effective and safe, adopt the disinfection hand cleanser product of modern biotechnology product processing.

Component of the present invention comprises surfactant, glycerol, antibacterial, adjuvant, deionized water.

Technical characteristics of the present invention be added have inexpensive, highly effective and safe, the antibacterial through modern biotechnology processing of All Pure Nature.Antibacterial is bacillus subtilis (Bacillus subtilis) fermentation broth extract, this bacterial strain has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on April 16th, 2012, the preservation address is positioned at the Yard 1, BeiChen xi Road, Chaoyang District, Beijing City No. 3, (postcode 100101), deposit number are CGMCC No.6012.

Fungus characteristic is as follows:

1. somatic cells is Gram-positive living in direct rod shape, spore or that near-end is given birth to.

2. colony characteristics is that white is circular, protuberance, and smooth surface, opaque, moistening, provoke adhesion.

3. can utilize various saccharides, as glucose, galactose, xylose, cellobiose, cottonseed sugar etc.; Produce amylase, protease, can gelatin hydrolysate, V.P, C.I. 13020. are positive.

4. the suitableeest cultivation temperature is 37 ℃, optimum pH 7.0.

This bacterium classification status is as follows: antibacterial territory (Bacteria); Firmicutes (Firmicutes); Bacillus cereus guiding principle (Bacillales); Bacillus cereus order (Bacillaceae); Bacillaceae (Bacillus); Bacillus (Bacillus); Bacillus subtilis strain (Bacillus subtilis).

In the present invention, the preparation method of fermentation broth extract is as follows:

The seed culture medium of bacterial strain is broth bouillon, Carnis Bovis seu Bubali cream 2g wherein, peptone 8g, NaCl 5g, water 1000ml, pH value 7.0 ~ 7.2, high pressure steam sterilization.

The seed liquor condition of culture: cultivation temperature is 33 ℃, and shaking speed is 140 rev/mins, and fermentation time is 13 hours.

Inoculum concentration is 8%, is conducive to the formation of antibacterial substance.

The fermentation fermentation medium consists of: glucose 0.3%, and peptone 1%, NaCl 0.5%, MgSO ₄.7H ₂O 0.7%, KH ₂PO ₄0.7%, pH8.0.

Fermentation culture conditions is: cultivation temperature is 33 ℃, and shaking speed is 140 rev/mins, and fermentation time is about 24 hours.

Extracting the antibacterial substance method from fermentation liquid is organic solvent precipitation method.

At first with the fermentation liquid high speed centrifugation, temperature 0-4 ℃, rotating speed is 8000 rev/mins, centrifugal ten minutes, keeps supernatant.

(methanol: ethanol=1:6), fully dissolving is placed in there-necked flask, and 40 degrees centigrade of temperature are controlled in water-bath, and mixing speed is 60rpm, and the time is 5 hours, collects organic facies, adopts vacuum distillation method to remove organic solvent to add the equal-volume organic solvent in supernatant.Vapo(u)rizing temperature 30-60 ℃, distilled 20 minutes, organic solvent is removed.The collecting precipitation material.Namely get antibacterial.

The crude extract of Fungicidal substance, after water dissolution, concentration is the 1%(mass volume ratio).Lysate can kill common pathogenic bacterium effectively.For example not only can effectively kill escherichia coli, Salmonella, the pathogenic microorganism that golden yellow common coccus etc. are common, and can kill the common coccus of methicillin-resistant staphylococcus.

method for making of the present invention is simple, deionized water adds in reactor, its temperature is heated to 65 ℃, then add successively glycerol (8 ~ 23%), surfactant (18 ~ 30%), speed of agitator is unsuitable too high, can be controlled in 20 ~ 30 rev/mins, after stirring, cool the temperature to 40 ℃, with citric acid, the pH value of solution is transferred to 6.5 ~ 7.0, antibacterial (0.1 ~ 1%), essence is a small amount of, adjuvant (1 ~ 5%), stir, speed of agitator can be controlled in 20 ~ 30 rev/mins, carry out the feed liquid that stirs degassed, adopt vacuum outgas, negative pressure can be controlled in 5Mpa, the bubble that removal forms in the stirring and dissolving process, reduce the air content of material, mixed liquor is carried out packing, can obtain handwashing liquid.Ratio accounts for the percentage by weight of product gross weight for each component herein.

Surfactant is sodium lauryl sulphate, Cortex cocois radicis oil base diglycollic amide, fatty alcohol-polyoxyethylene ether, betanin, Sodium Alcohol Ether Sulphate or its mixture.

Adjuvant is polyethylene glycol stearate diester etc.

Essence used can add according to the odor type of product, makes product have different odor types, satisfies the needs of different consumers.

The invention has the beneficial effects as follows: the present invention has not only realized the function of handwashing liquid removal dirt, and the function of good sterilization and sterilization, particularly can kill the characteristic of methicillin-resistant staphylococcus aureus.The emergency toxicology experiment has also verified the antibacterial that adds in this detergent people and animals to be had very high safety.Scientist has found methicillin-resistant staphylococcus aureus in 1961, find that at present it spreads all over the world, and becomes important infectious bacteria, has brought great threat for people's health.The production of the antibacterial that adds in handwashing liquid utilizes fermentation raw material with low cost, adopts modern biotechnology, and technique is simple, and is with low cost.Make the present invention, become a kind of sterilizing power strong, safety, cheapness, handwashing liquid with high content of technology.

That adds in the present invention has the antiseptical function by the pure natural fermentation raw material through the antibacterial that modern biotechnology processes, and has characteristics safely and efficiently.Need not to add again antiseptic in handwashing liquid.

The specific embodiment

Induction mutation of bacterium

Take the ultraviolet mutagenesis method: take CICC10023 as starting strain, make bacteria suspension, coat on the culture medium plate, cultivate in 37 ℃ of incubators after ultra-vioket radiation 90s.

Bacterial screening

Choose 30 single bacterium colonies of looking strong from the 90s plate, be inoculated on the inclined-plane, in 37 ℃ of incubators, cultivated 0--4 ℃ of stored refrigerated 24 hours; With original strain in contrast, adopt the conventional herd breeding condition, sodium carboxymethyl cellulose is as carbon source and Proteolytic enzyme circle method screening cellulase-producing and the strong bacterial strain of protease, screening obtains cellulase-producing and strong bacterial strain bacillus subtilis (Bacillus subtilis) L1 of protease, this bacterial strain cellulase-producing can improve 25% by the force rate original strain, produces protease and can improve 52% by the force rate original strain.In the center preservation of Chinese common micro-organisms culture presevation administration committee's common micro-organisms, deposit number is CGMCC No 6012 with this strain.

This product bacillus subtilis used (Bacillus subtilis) strain, this bacterial strain has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on April 16th, 2012, the preservation address is positioned at the Yard 1, BeiChen xi Road, Chaoyang District, Beijing City No. 3, (postcode 100101), deposit number are CGMCC No6012.

Studies show that by experiment: antibacterial have good inhibition escherichia coli, staphylococcus aureus, etc. the characteristics of pathogenic bacterium.

The invention will be further described below in conjunction with specific embodiment, but be not limited to embodiment.

Embodiment 1

prepare 100 kilograms of products, the 50kg deionized water is added in reactor, its temperature is heated to 65 ℃, then add successively 9kg glycerol, the 9kg sodium lauryl sulphate, the 5kg fatty alcohol-polyoxyethylene ether, the 5kg Sodium Alcohol Ether Sulphate, speed of agitator is unsuitable too high, can be controlled in 20 ~ 30 rev/mins, after stirring, cool the temperature to 40 ℃, with citric acid, the pH value of solution is transferred to 6.5 ~ 7.0, 0.2kg antibacterial, 0.0001kg rose essence, the 2kg polyethylene glycol mono stearate, insufficient section deionized water polishing, stir, speed of agitator can be controlled in 20 ~ 30 rev/mins, carry out the feed liquid that stirs degassed, adopt vacuum outgas, negative pressure can be controlled in 5Mpa, the bubble that removal forms in the stirring and dissolving process, reduce the air content of material, mixed liquor is carried out packing, can obtain handwashing liquid.

Product of the present invention adopts conventional microorganism detection bactericidal effect, and result of the test shows, product bactericidal effect of the present invention reaches more than 85%, and bactericidal effect is obvious.Escherichia coli, Salmonella, golden yellow common coccus are selected in microorganism.

Embodiment 2

prepare 100 kilograms of products, the 60kg deionized water is added in reactor, its temperature is heated to 65 ℃, then add successively 12kg glycerol, the 9kg sodium lauryl sulphate, the 6kg fatty alcohol-polyoxyethylene ether, the 5kg Sodium Alcohol Ether Sulphate, 25 rev/mins of speeds of agitator, after stirring, cool the temperature to 40 ℃, with citric acid, the pH value of solution is transferred to 6.5 ~ 7.0, 0.6kg antibacterial, 0.0003kg rose essence, the 1kg polyethylene glycol mono stearate, insufficient section deionized water polishing, stir, speed of agitator can be controlled in 20 ~ 30 rev/mins, carry out the feed liquid that stirs degassed, adopt vacuum outgas, negative pressure can be controlled in 5Mpa, the bubble that removal forms in the stirring and dissolving process, reduce the air content of material, mixed liquor is carried out packing, can obtain handwashing liquid.

Claims (5)

1. a handwashing liquid, comprise surfactant, glycerol, adjuvant, deionized water, it is characterized in that, contains antibacterial in described handwashing liquid, and described antibacterial is the fermentation broth extract of bacillus subtilis CGMCC6012.

2. handwashing liquid according to claim 1, is characterized in that, described antibacterial part by weight is 0.1 ~ 1%.

3. handwashing liquid preparation method according to claim 1, it is characterized in that, comprise the steps: that deionized water adds in reactor, be heated to 65 ℃, add glycerol, surfactant, stir, be cooled to 40 ℃, pH value transfers to 6.5 ~ 7.0, adds antibacterial, essence and adjuvant, stirs, degassed, packing.

4. handwashing liquid preparation method according to claim 3, is characterized in that, described adjuvant is polyethylene glycol stearate diester.

5. handwashing liquid preparation method according to claim 3, prepare 100 kilograms of products, the 50kg deionized water is added in reactor, its temperature is heated to 65 ℃, then add successively 9kg glycerol, the 9kg sodium lauryl sulphate, the 5kg fatty alcohol-polyoxyethylene ether, the 5kg Sodium Alcohol Ether Sulphate, speed of agitator is unsuitable too high, can be controlled in 20 ~ 30 rev/mins, after stirring, cool the temperature to 40 ℃, with citric acid, the pH value of solution is transferred to 6.5 ~ 7.0, 0.2kg antibacterial, 0.0001kg rose essence, the 2kg polyethylene glycol mono stearate, insufficient section deionized water polishing, stir, speed of agitator can be controlled in 20 ~ 30 rev/mins, carry out the feed liquid that stirs degassed, adopt vacuum outgas, negative pressure can be controlled in 5Mpa, the bubble that removal forms in the stirring and dissolving process, reduce the air content of material, mixed liquor is carried out packing, can obtain handwashing liquid.