CN101514310B - High-efficiency bactericidal liquid detergent - Google Patents
High-efficiency bactericidal liquid detergent Download PDFInfo
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- CN101514310B CN101514310B CN2008101017682A CN200810101768A CN101514310B CN 101514310 B CN101514310 B CN 101514310B CN 2008101017682 A CN2008101017682 A CN 2008101017682A CN 200810101768 A CN200810101768 A CN 200810101768A CN 101514310 B CN101514310 B CN 101514310B
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Abstract
The invention discloses a high-efficiency bactericidal liquid detergent, and belongs to the field of sanitary products. The invention aims to provide a high-efficiency and safe product processed by modern biotechnology product. The high-efficiency bactericidal liquid detergent achieves the function of removing dirt of clothes, has outstanding washing ability, strong detergency and convenient use,particularly has good bactericidal and disinfectant functions and has the property of killing methicillin-resistant Staphylococcus aureus. The production of a bactericide added in the liquid detergent utilizes ferment raw materials with low cost, adopts the modern biotechnology, and has simple process and low cost. Therefore, the high-efficiency bactericidal liquid detergent has strong disinfection capacity, safety, low price and high technology content.
Description
Technical field:
The invention belongs to field of hygiene products, relate to special washing liquid.
Background technology:
The washing liquid of selling on the market mainly contains the function of removing the clothing dirt.Along with the more and more attention of people, has the washing liquid of disinfection function to health.At present, the washing liquid of selling on the market is normally by several tensio-active agents, and the adding washing assistant processes.This class washing liquid has only the effect of washing usually, can not the bacterium on the clothing effectively be killed.At present, locate in family, hotel, railway, airport, school etc., washing machine has obtained using widely.If the easy breed bacteria of washing machine not to its disinfection, then causes people's infection easily, and people's health is brought threat.
On the market, some have also occurred and had the washing liquid of disinfecting and sterilizing functions.Mainly be to have added some sterilizing agents and Chinese medicinal materials etc., make washing powder have certain germicidal efficacy.These sterilizing agents, some is not really safe to people, if rinsing is unclean, people's skin is had certain stimulation.Some sterilizing agent costs an arm and a leg, and is unfavorable for reducing production costs and increasing competitiveness of product.At present, it is strong to be starved of a kind of soil removability on the market, and sterilizing power is strong, safety, cheap washing liquid.
At present, the disinfection washing liquid great majority of selling on the market are to have added chemical bactericide.At present, in the market, having the washing liquid of sterilizing function, mainly is to have the characteristic of killing common intestinal bacteria, Salmonellas and streptococcus aureus
The purpose of this invention is to provide a kind of highly effective and safe, adopt the bactericidal liquid detergent product of modern biotechnology product processing.
Prescription of the present invention mainly contains: sterilant, anion surfactant, nonionogenic tenside, amphoterics, whitening agent, Chinese honey locust distillate, essence, deionized water.
Major technique feature of the present invention be added have inexpensive, highly effective and safe, the sterilant through modern biotechnology processing of All Pure Nature.The used sterilant of the present invention is the inventor's an invention achievement.Sterilant is subtilis LD-8 (Bacillus subtilis LD-8) fermentation broth extract, culture presevation is in China Microbial Culture Preservation Commission common micro-organisms center, Datun Road, Chaoyang District, Beijing City, preservation address, preservation date on December 18th, 2007, culture presevation numbering CGMCC 2294.
Fungus characteristic is as follows:
1. somatic cells is life or the living Gram-positive of near-end in direct rod shape, the gemma.
2. colony characteristics is that white is circular, protuberance, and smooth surface, opaque, moistening, provoke adhesion.
3. can utilize various saccharides, as glucose, semi-lactosi, wood sugar, cellobiose, raffinose etc.; Produce amylase, proteolytic enzyme, can gelatin hydrolysate, V.P, the methyl red positive.
4. 37 ℃ of the suitableeest culture temperature, optimal pH 7.0.
This bacterium classification status is as follows: bacterium territory (Bacteria); Firmicutes (Firmicutes); Genus bacillus guiding principle (Bacillales); Genus bacillus order (Bacillaceae); Bacillaceae (Bacillus); Bacillus (Bacillus); Bacillus subtilis bacterial classification (Bacillus subtilis).
The sterilant addition is product weight (0.1～1%).
The preparation method of fermentation broth extract is as follows among the present invention:
The seed culture medium of bacterial strain is a broth culture, extractum carnis 3g wherein, peptone 10g, NaCl 5g, water 1000ml, pH value 7.0～7.2, high pressure steam sterilization.
The seed liquor culture condition: culture temperature is 37 ℃, and shaking speed is 140 rev/mins, and fermentation time is 10～14 hours.Inoculum size is 6%～10% o'clock, helps the formation of antibacterial substance.
The fermention medium of optimizing consists of: sucrose 0.3%, and peptone 1%, NaCl 0.5%, MgSO
4.7H
2O 0.7%, KH
2PO
40.7%, pH8.0.
The fermentation culture conditions of optimizing is: culture temperature is 37 ℃, and shaking speed is 140 rev/mins, and fermentation time is about 24 hours.
Extracting the antibacterial substance method from fermented liquid is organic solvent precipitation method.The available organic solvent is acetone, methyl alcohol, ethanol.
At first with fermented liquid, add supercentrifuge, temperature 0-4 ℃, rotating speed is 10000 rev/mins, centrifugal ten minutes, remove precipitation, and keep supernatant liquor.
With the supernatant that obtains, add isopyknic methyl alcohol, the temperature of system is reduced to-5 ℃ rapidly, preserved 2 hours for-5 ℃.Collect organic phase, adopt vacuum distillation method to remove organic solvent.Distillation temperature 30-60 ℃, distilled 20 minutes, organic solvent is removed.The collecting precipitation material.This promptly is the crude extract of our bacteriocidal substance that obtains.
The crude extract of bacteriocidal substance, after water dissolution, concentration is 1% (mass volume ratio).Lysate can kill common pathogenic bacterium effectively.For example not only can kill intestinal bacteria effectively, Salmonellas, the pathogenic micro-organism that golden yellow common coccus etc. are common, and can kill the common coccus of methicillin-resistant staphylococcus.
This disinfecting liquid has high security, in the emergency toxicology experiment, we gavage 5 groups of 200 mouse with this disinfecting liquid, and the mouse of experimental group none occur dead, and subtilis is present in occurring in nature widely, and many important industrial bacterial strains are exactly subtilis.Substantially can conclude that this disinfecting liquid is safe for the user, can relievedly use.After adding it to washing liquid, find that it still has good fungicidal activity.
Method for making of the present invention is simple, add and at first deionized water (20～45%) is added in the reactor man-hour, its temperature is heated to 70 ℃, add anion surfactant (10～15%) then successively, nonionogenic tenside (20～30%), amphoterics (8～15%) is after whitening agent (0.2～0.5%) stirs, cool the temperature to 40 ℃, with citric acid the pH value of solution is transferred to 6.5～7.0, add Chinese honey locust distillate (10～20%), sterilant (0.1～1%), essence, stir, mixing speed can be controlled in 20～30 rev/mins, and the feed liquid that stirs is outgased, adopt vacuum outgas, negative pressure can be controlled in 5Mpa, removes the bubble that forms in the stirring and dissolving process, reduces the air content of material, mixed solution is carried out packing, can obtain high-efficiency bactericidal liquid detergent.Ratio is the weight percent of each ingredients constitute product gross weight herein.
Anion surfactant is a sodium lauryl sulphate, and is at least a in the fat alcohol polyethylene ether sodium sulfate.
Described nonionogenic tenside can be at least a in alkylphenol polyoxyethylene, the fatty alcohol-polyoxyethylene ether.
Amphoterics is at least a in Varion CDG-K or the dodecyl dimethyl ammonium oxide.
The Chinese honey locust distillate, with an amount of Chinese honey locust, add 4 times of water loggings bubble after, distill, obtain the distillate suitable with the Chinese honey locust quality.
Whitening agent is a sodium ethylene diamine tetracetate.
Used essence can add according to the odor type of product, makes product have different odor types, satisfies different human consumers' needs.
Beneficial effect:
The present invention realized that not only washing liquid removes the function of dirt on the clothing, and washability is outstanding, strong detergency, easy to use, especially possessed good sterilization and disinfectant function, particularly can kill the characteristic of methicillin-resistant staphylococcus aureus.The emergency toxicology experiment has also verified the sterilant that adds in this washing liquid people and animals to be had very high security.Scientist has found methicillin-resistant staphylococcus aureus in 1961, find that at present it spreads all over the world, and becomes important infectious bacteria, has brought great threat for people's health.The production of the sterilant that adds in the washing liquid utilizes fermentation raw material with low cost, adopts modern biotechnology, and technology is simple, and is with low cost.Make the present invention, it is strong to become a kind of sterilizing power, safety, cheapness, washing liquid with high content of technology.
That adds among the present invention has the rot-resistant function by the pure natural fermentation raw material through the sterilant that modern biotechnology processes, and has characteristics safely and efficiently.Need not to add again sanitas in the liquid detergent.
Specific embodiment: the invention will be further described below in conjunction with specific embodiment, but be not limited to embodiment.
100 kilograms of products of embodiment 1 preparation: the 45kg deionized water is added in the reactor, its temperature is heated to 70 ℃, add 10kg fat alcohol polyethylene ether sodium sulfate then successively, the 20kg fatty alcohol-polyoxyethylene ether, the 10kg Varion CDG-K, 0.3kg after sodium ethylene diamine tetracetate stirs, cool the temperature to 40 ℃, the pH value of solution transferred to 6.5～7.0 with citric acid, add 14kg Chinese honey locust distillate, 0.2kg sterilant, the 0.0001kg rose compound stirs, mixing speed can be controlled in 20～30 rev/mins, the feed liquid that stirs is outgased, adopt vacuum outgas, negative pressure can be controlled in 5Mpa, the bubble that removal forms in the stirring and dissolving process, reduce the air content of material, mixed solution is carried out packing, can obtain high-efficiency bactericidal liquid detergent.
Get 9% concentration product of the present invention, adopt conventional microorganism detection sterilization effect, test-results shows that product sterilization effect of the present invention reaches more than 90%, and sterilization effect is obvious.Microorganism is selected intestinal bacteria, Salmonellas, golden yellow common coccus.
Claims (6)
1. a bactericidal liquid detergent comprises anion surfactant, nonionogenic tenside, amphoterics, whitening agent, the Chinese honey locust distillate is characterized in that containing sterilant, the bactericidal liquid detergent preparation method is as follows: deionized water adds in the reactor, be heated to 70 ℃, the adding weight percent is 10～15% anion surfactant, 20～30% nonionogenic tenside, 8～15% amphoterics, 0.2～0.5% whitening agent stirs, and is cooled to 40 ℃, the pH value transfers to 6.5～7.0, the Chinese honey locust distillate of adding 10～20%, 0.1～1% sterilant and essence stir, the degassing, packing; Ratio is the weight percent of each ingredients constitute product gross weight herein; Described sterilant is the fermentation broth extract of subtilis LD-8 (Bacillussubtilis LD-8) CGMCC2294, and the fermentation broth extract preparation method of described CGMCC2294 is as follows:
The seed culture medium of bacterial strain is a broth culture, extractum carnis 3g wherein, peptone 10g, NaCl 5g, water 1000ml, pH value 7.0～7.2, high pressure steam sterilization; The seed liquor culture condition: culture temperature is 37 ℃, and shaking speed is 140 rev/mins, and fermentation time is 10～14 hours, and inoculum size is 6%～10%; Fermention medium consists of: sucrose 0.3%, and peptone 1%, NaCl 0.5%, MgSO
47H
2O 0.7%, KH
2PO
40.7%, pH8.0; Fermentation culture conditions is: culture temperature is 37 ℃, and shaking speed is 140 rev/mins, and fermentation time is 24 hours;
At first with fermented liquid, add supercentrifuge, temperature 0-4 ℃, rotating speed is 10000 rev/mins, centrifugal ten minutes, remove precipitation, and keep supernatant liquor;
With the supernatant liquor that obtains, add isopyknic methyl alcohol, the temperature of system is reduced to-5 ℃ rapidly, preserved 2 hours for-5 ℃; Collect organic phase, adopt vacuum distillation method to remove organic solvent; Distillation temperature 30-60 ℃, distilled 20 minutes, organic solvent is removed the collecting precipitation material.
2. according to the preparation method of the described bactericidal liquid detergent of claim 1, its preparation method is as follows: deionized water adds in the reactor, is heated to 70 ℃, the adding weight percent is 10～15% anion surfactant, 20～30% nonionogenic tenside, 8～15% amphoterics, 0.2～0.5% whitening agent stirs, and is cooled to 40 ℃, the pH value transfers to 6.5～7.0, the Chinese honey locust distillate of adding 10～20%, 0.1～1% sterilant and essence stir, the degassing, packing.
3. according to the preparation method of the described bactericidal liquid detergent of claim 2, it is characterized in that described anion surfactant is sodium lauryl sulphate, fat alcohol polyethylene ether sodium sulfate or both mixtures.
4. according to the preparation method of the described bactericidal liquid detergent of claim 2, it is characterized in that described nonionogenic tenside is at least a in alkylphenol polyoxyethylene, the fatty alcohol-polyoxyethylene ether.
5. according to the preparation method of the described bactericidal liquid detergent of claim 2, it is characterized in that described amphoterics is at least a in Varion CDG-K or the dodecyl dimethyl ammonium oxide.
6. according to the preparation method of the described bactericidal liquid detergent of claim 2, it is characterized in that described whitening agent is a sodium ethylene diamine tetracetate.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008101017682A CN101514310B (en) | 2008-03-12 | 2008-03-12 | High-efficiency bactericidal liquid detergent |
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| CN2008101017682A CN101514310B (en) | 2008-03-12 | 2008-03-12 | High-efficiency bactericidal liquid detergent |
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| CN101514310A CN101514310A (en) | 2009-08-26 |
| CN101514310B true CN101514310B (en) | 2011-02-02 |
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| CN2008101017682A Active CN101514310B (en) | 2008-03-12 | 2008-03-12 | High-efficiency bactericidal liquid detergent |
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Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102061226B (en) * | 2009-11-13 | 2013-02-06 | 上海卫安生物科技有限公司 | Liquid detergent |
| CN103205318A (en) * | 2012-07-14 | 2013-07-17 | 邵素英 | Laundry detergent |
| CN103146508A (en) * | 2012-07-14 | 2013-06-12 | 邵素英 | Detergent |
| CN103160387A (en) * | 2013-01-04 | 2013-06-19 | 韩刚 | Metal and nonmetal surface degreasing cleaning agent with zero discharge |
| CN104804894A (en) * | 2015-04-14 | 2015-07-29 | 江南大学 | Environment-friendly compound laundry detergent with sterilizing and anti-fungus functions and preparation method of compound laundry detergent |
| CN105670824A (en) * | 2016-01-12 | 2016-06-15 | 洛阳和梦科技有限公司 | Environment-friendly sterilizing liquid laundry detergent |
| CN106753930A (en) * | 2016-12-05 | 2017-05-31 | 广西大学 | A kind of liquid detergent |
| CN108531309A (en) * | 2018-05-21 | 2018-09-14 | 李均辉 | A kind of color protection eliminates the unusual smell liquid detergent and its preparation method and application |
| CN108774602A (en) * | 2018-05-30 | 2018-11-09 | 王成 | A kind of Antibacterial laundry detergent |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1432284A (en) * | 2002-01-18 | 2003-07-30 | 福建泰禾生化科技股份有限公司 | High-efficiency biological germicide and its prepn process |
| CN1766091A (en) * | 2005-09-23 | 2006-05-03 | 中国农业大学 | Bacillus subtilis and its uses |
| CN101041846A (en) * | 2007-01-29 | 2007-09-26 | 中国科学院等离子体物理研究所 | Preparation method and usage for novel lipopeptide type biosurfactant surfactin |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1432284A (en) * | 2002-01-18 | 2003-07-30 | 福建泰禾生化科技股份有限公司 | High-efficiency biological germicide and its prepn process |
| CN1766091A (en) * | 2005-09-23 | 2006-05-03 | 中国农业大学 | Bacillus subtilis and its uses |
| CN101041846A (en) * | 2007-01-29 | 2007-09-26 | 中国科学院等离子体物理研究所 | Preparation method and usage for novel lipopeptide type biosurfactant surfactin |
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Effective date of registration: 20120504 Address after: 100029, Beijing, Xicheng District Middle East Building No. 26, 3 door, room 402 Patentee after: Ning Yuesheng Address before: 101121, 1 Cui Lu, Liyuan, Tongzhou District, Beijing, Zhenjiang Patentee before: Beijing Ludaoyuan Bee Products Co., Ltd. |
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Effective date of registration: 20210105 Address after: 102200 1618, unit 1, 16 / F, building 2, 87 Jiancai Chengxi Road, Huilongguan town, Changping District, Beijing Patentee after: Beijing Huasheng Justice Technology Development Co.,Ltd. Address before: 100029 room 402, door 3, building 26, yuzhongdong Li, Xicheng District, Beijing Patentee before: Ning Yuesheng |