CN103155745A - Method for enhancing germination rate of safflower seed by utilizing multi-wall carboxylic carbon nanotube - Google Patents
Method for enhancing germination rate of safflower seed by utilizing multi-wall carboxylic carbon nanotube Download PDFInfo
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- CN103155745A CN103155745A CN2013100863270A CN201310086327A CN103155745A CN 103155745 A CN103155745 A CN 103155745A CN 2013100863270 A CN2013100863270 A CN 2013100863270A CN 201310086327 A CN201310086327 A CN 201310086327A CN 103155745 A CN103155745 A CN 103155745A
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- seed
- many walls
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- germination
- radix isatidis
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- Y02P60/216—
Abstract
The invention provides a method for enhancing germination rate of safflower seeds by utilizing multi-wall carboxylic carbon nanotubes. The method comprises the following steps of: A, harvesting seeds; B, preprocessing the seeds; C, formulating multi-wall carboxylic carbon nanotubes; D, soaking the seeds with a multi-wall carboxylic carbon nanotube solution; E, planting the seeds; and E, recording data. According the method, the germination rate of the safflower seeds is enhanced by 10%, so that the production cost of the safflower medicine is reduced.
Description
Technical field
The present invention relates to a kind of method of traditional Chinese medicine seed sprouting, belonged to traditional Chinese medicine cultivation production technical field.
Background technology
Safflower another name safflower is composite family annual herb plant, with flower, seedling, fruit people medicine, be used as medicine have activating blood to promote menstruation, the effect of blood stasis removing analgesic, be used for through closing, dysmenorrhoea, lochia, note of the ancient Chinese lump in the abdomen lump in the abdomen, injury from falling down, sore swell and ache etc.It contains reddish yellow pigment and carthamin, and is at present clinical in diseases such as metryperemia, thrombus of heart blood vessel formation; Also do pain relieving, antiphlogistic etc.Nano material is that crystalline size is less than monocrystal or the polycrystal of 100nm.After wherethrough reason (activation), can change arrangement mode and the energy state of hydrone, make that water clusters diminishes, energy improves, the active increase, produce many special physico chemistry and biological effect, change the function of water and other materials and organism, as the solvability that increases water, the saturating property of cell biological that improves water etc.CNT can penetrate Structure of Seed-coat, appreciable impact seed germination, thereby the growth of promotion plant.
Summary of the invention
The object of the present invention is to provide a kind of method of utilizing many walls carboxylation CNT to improve the Semen Carthami germination rate, inquire into nano material to the impact of germination of safflower seed, seek the method that helps germination of safflower seed, thereby improve the production efficiency of safflower.
The main feature that the nano material of utilizing this kind improves Semen Carthami germination rate method is:
Gathering of A, seed: gather when Semen Carthami is ripe autumn, after drying, and is placed in 4 ℃ of refrigerators and preserves, standby.
B, seed pre-treatment: select in the same size, plant the unabroken Semen Carthami of skin, with 0.1% clorox surface sterilization 10min, deionized water rinsing 5 times, standby.
The preparation of C, many walls carboxylation carbon nano-tube solution: with the abundant grind into powder of many walls carboxylation CNT, be mixed with the 200mg/L mother liquor standby.
D, seed soaking: the Semen Carthami that will sterilize is put into the beaker of many walls carboxylation carbon nano-tube solution that 20mg/L is housed, seed soaking 12h.
E, sowing: with peat soil: perlite=2: 1 adds carbendazim and compound fertilizer as the matrix of germinateing before sowing, after mixing thoroughly, upper basin sowing is placed in the greenhouse.
F, germination record: after the beginning of germinateing, the normal seedling number that every day, record was sprouted is until till occurring without germination seed.The germination standard approximates the length of seed self as germination take the prominent plumular axis length that breaks in the seed coat.After the sprouting of 15 days, reach 87% by the percentage of seedgermination of many walls carboxylation carbon nano-tube solution seed soaking, contrast only 77%, germination rate has improved 10 percentage points.
Embodiment
Safflower germination example explanation the specific embodiment of the present invention after processing below in conjunction with nano material.
Gathering and pre-treatment of example 1 seed
1, gather when Semen Carthami is ripe autumn, peel off the angle pericarp, after drying, and be placed in 4 ℃ of refrigerators and preserve.
2, select in the same size, unabroken Semen Carthami, after 0.1% clorox surface sterilization 10min, distilled water repeatedly rinses several times on a small quantity, extremely without till the clorox taste.Jog several in disinfecting process is in order to improve Disinfection Effect.
The seed soaking of example wall more than 2 carboxylation carbon nano-tube solution
Nano material is processed: the abundant grind into powder of many walls carboxylation CNT, be dissolved in deionized water, and ultrasonic oscillation 30min, and add a small amount of DMF dispersant hydrotropy, then constant volume, be mixed with the 200mg/L mother liquor standby.
Example 3 Semen Carthami sowings
1, seed soaking: the seed after sterilization is put into the beaker of many walls carboxylation carbon nano-tube solution that 10 times of dilutions are housed.
Claims (2)
1. method of utilizing many walls carboxylation CNT to improve the Radix Isatidis percentage of seedgermination is characterized in that:
Gathering of A, seed: gather during the Radix Isatidis seed maturity autumn, after drying, and is placed in 4 ℃ of refrigerators and preserves, standby.
B, seed pre-treatment: select in the same size, plant the unabroken Radix Isatidis seed of skin, with 0.1% clorox surface sterilization 10min, deionized water rinsing 5 times, standby.
The preparation of C, many walls carboxylation carbon nano-tube solution: with the abundant grind into powder of many walls carboxylation CNT, be mixed with the 200mg/L mother liquor standby.
D, seed soaking: the Radix Isatidis seed that will sterilize is put into the beaker of many walls carboxylation carbon nano-tube solution that 20mg/L is housed, and seed soaking 24h is placed in the beaker sealing on shaking table during seed soaking, and seed is fully contacted with solution.
E, sowing: with peat soil: perlite=2: 1 adds carbendazim and compound fertilizer as the matrix of germinateing before sowing, after mixing thoroughly, upper basin sowing is placed in the greenhouse.
F, germination record: after the beginning of germinateing, the normal seedling number that every day, record was sprouted is until till occurring without germination seed.The germination standard approximates the length of seed self as germination take the prominent plumular axis length that breaks in the seed coat.After the sprouting of 15 days, the percentage of seedgermination of being soaked seed by many walls carboxylation carbon nano-tube solution reaches 79%.
2. utilize according to claim 1 many walls carboxylation carbon nanomaterial to improve the method for Radix Isatidis percentage of seedgermination, it is characterized in that in step B, seed prior to seeding, be the 24h that soaks seed in many walls carboxylation carbon nano-tube solution of 20mg/L prior to concentration, during seed soaking, the beaker sealing is placed on shaking table, seed is fully contacted with solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013100863270A CN103155745A (en) | 2013-03-19 | 2013-03-19 | Method for enhancing germination rate of safflower seed by utilizing multi-wall carboxylic carbon nanotube |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2013100863270A CN103155745A (en) | 2013-03-19 | 2013-03-19 | Method for enhancing germination rate of safflower seed by utilizing multi-wall carboxylic carbon nanotube |
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| CN103155745A true CN103155745A (en) | 2013-06-19 |
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Family Applications (1)
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| CN2013100863270A Pending CN103155745A (en) | 2013-03-19 | 2013-03-19 | Method for enhancing germination rate of safflower seed by utilizing multi-wall carboxylic carbon nanotube |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105875121A (en) * | 2014-12-02 | 2016-08-24 | 江苏苏美仑智能科技有限公司 | Method for promoting postharvest flowering and freshness keeping of lilies through multi-wall carbon nanotubes |
| CN106717279A (en) * | 2016-12-26 | 2017-05-31 | 新昌县钧国生物技术有限公司 | The method that Semen Carthami germination percentage is improved using many wall carboxylation CNTs |
| CN111183900A (en) * | 2020-01-19 | 2020-05-22 | 贵州大学 | Method for efficiently obtaining aseptic safflower seedlings |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2006113688A2 (en) * | 2005-04-19 | 2006-10-26 | Kamterter Ii, L.L.C. | Systems for control, use of fluids, particles |
| CN101822140A (en) * | 2010-05-07 | 2010-09-08 | 中国农业科学院生物技术研究所 | Method for using nanometer materials for promoting sprouting and growth of soybean seeds under drought stress |
| CN102228007A (en) * | 2011-06-15 | 2011-11-02 | 中国农业科学院生物技术研究所 | Tissue culture method for promoting differentiation and regeneration of soybean cotyledon node explant by using nano material |
-
2013
- 2013-03-19 CN CN2013100863270A patent/CN103155745A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006113688A2 (en) * | 2005-04-19 | 2006-10-26 | Kamterter Ii, L.L.C. | Systems for control, use of fluids, particles |
| CN101822140A (en) * | 2010-05-07 | 2010-09-08 | 中国农业科学院生物技术研究所 | Method for using nanometer materials for promoting sprouting and growth of soybean seeds under drought stress |
| CN102228007A (en) * | 2011-06-15 | 2011-11-02 | 中国农业科学院生物技术研究所 | Tissue culture method for promoting differentiation and regeneration of soybean cotyledon node explant by using nano material |
Non-Patent Citations (3)
| Title |
|---|
| MARIYA KHODAKOVSKAYA 等: "Carbon Nanotubes Are Able To Penetrate Plant Seed Coat and Dramatically Affect Seed Germination and Plant Growth", 《ACS NANO》 * |
| 何先元 等: "红花种子发芽检验标准化研究", 《安徽农业科学》 * |
| 吕继涛 等: "人工纳米材料与植物的相互作用:植物毒性吸收和传输", 《化学进展》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105875121A (en) * | 2014-12-02 | 2016-08-24 | 江苏苏美仑智能科技有限公司 | Method for promoting postharvest flowering and freshness keeping of lilies through multi-wall carbon nanotubes |
| CN106717279A (en) * | 2016-12-26 | 2017-05-31 | 新昌县钧国生物技术有限公司 | The method that Semen Carthami germination percentage is improved using many wall carboxylation CNTs |
| CN111183900A (en) * | 2020-01-19 | 2020-05-22 | 贵州大学 | Method for efficiently obtaining aseptic safflower seedlings |
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Application publication date: 20130619 |