CN103142592B - Medicine for preventing fish saprolegniasis and application thereof - Google Patents
Medicine for preventing fish saprolegniasis and application thereof Download PDFInfo
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- CN103142592B CN103142592B CN201310039304.4A CN201310039304A CN103142592B CN 103142592 B CN103142592 B CN 103142592B CN 201310039304 A CN201310039304 A CN 201310039304A CN 103142592 B CN103142592 B CN 103142592B
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Abstract
The invention relates to medicine for preventing fish saprolegniasis and application thereof. The medicine is combination of one or more types of the following materials: industrial biocide containing bromine, pyridine thioketone salts, 3-iodo-2-propynyl-butyl-carbamate, phenol derivatives, compound containing aldehyde group, mildew preventive 10, 10' oxybis-phenoxarsine, prepared rehmannia roots, garden burnet, pomegranate bark, dyer woad leaves and artemisia carvifolia. The medicine is generally low in a minimal inhibitory concentration to saprolegnia bacterial strains, and is suitable for prevention of the saprolegniasis in aquiculture. The Chinese herbal medicine and composition thereof can prevent the saprolegniasis and have the advantages of being free of pollution and a toxic side effect. The medicine is especially suitable for prevention of the saprolegniasis of aquatic livestock of high economic value, such as ornamental fishes. Food security can be effectively ensured, and quality of aquatic products can be improved.
Description
Technical field
The present invention relates to for preventing and treating the medicine of aquatic animal fungal disease, specifically, is a kind of medicine and application thereof that prevents and treats Fish fish molds.
Background technology
Water mo(u)ld is the general name of a class filamentous fungi, it is the cause of disease of the fungal disease of the common harm aquatic animal of a class and ovum thereof, the disease being caused by this class fungus is called fish molds, this disease does not have strict selectivity to the kind of aquatic animal, popular wide, and all can fall ill throughout the year, can cause the heavy losses wild and class that cultivates fresh water fishes.
With malachite green oxalate, treat fish molds aborning for a long time always, and obtained good curative effect, but because this medical instrument has " three cause ", high residue, is difficult for the defects such as degraded, thereby China in 2002 prohibites and in aquaculture, uses malachite green oxalate.And the nuisanceless medicine that can compare favourably with it in curative effect, economically at present never comes out, cause and in breeding production, occurred the serious disappearance to the effective therapeutical effect medicine of fish molds.So, cause the good medicine of inefficacy in production to use on the one hand, to breeding production, caused larger economic loss, affected the sound development of culture fishery; On the other hand, some illegal use malachite green oxalate, causes a hidden trouble to food safety, has directly endangered public food safety, also has influence on the outlet of China's aquatic products simultaneously.Therefore no matter be, in order to meet the actual needs of aquiculture disease control, or for Safety of Aquatic Products, the medicine of developing safety, high-efficiency prevention and control fish molds is all very urgent.
Chinese patent literature CN 201110092891.4, and the applying date: 2011-04-13 provides a kind of method with methylene blue treatment fish body fish molds, and first the method is made a definite diagnosis after Fish seed trouble fish molds, by methylene blue water dissolution, then presses 2-4g/m
3the full pond float of water body final concentration, and can be used in conjunction 2 times depending on the state of an illness; Chinese patent literature CN 200510047346.8, the applying date: 2005-09-29, a kind of fish, shrimp, Eriocheir sinensis antifungal Chinese herbal and crude drugs preparations are disclosed, it is characterized in that each weight percentages of components is: Galla Chinensis 40～70%, Cortex Phellodendri 10～30%, Cortex Moutan 10～30%, Herba Houttuyniae 10～30%, after decocting for the dipping of fish body, fish roe or stifling, it is bacteriostasis and sterilization, antiviral, parasite killing directly, do not produce drug resistance, and can regulate and improve immunity of organisms, strengthen the resistance against diseases of animal body.But on the whole, the medicament categories of control saprolegniasis of aquatic animals is still less at present, often limited in practical application, therefore find the medicine of more safety, high-efficiency prevention and control fish molds, to reduce the economic loss of breeding production and to stop food safety hidden danger, be very necessary.
Summary of the invention
The object of the invention is for deficiency of the prior art, a kind of medicine of preventing and treating Fish fish molds is provided.
Another object of the present invention is that the purposes of said medicine is provided.
For achieving the above object, the technical scheme that the present invention takes is:
A kind of medicine of preventing and treating Fish fish molds, described medicine is selected from any one or several combination in following: brominated industrial bactericide, pyrithione salt, iodo propinyl compound, the phenol analog derivative that contains compound structure (I), the compound that contains aldehyde radical, antifungus agent 10, the two fen arsenic (OBPA) of 10'-oxo, Radix Rehmanniae Preparata, Radix Sanguisorbae, Pericarpium Granati, Folium Isatidis, Herba Artemisiae Annuae;
(I) is as follows for described compound structure:
（I）
Wherein, R is the group of ortho position on phenyl ring, a position, para-position, and its architectural feature is functional group-COOC
nh
2n+1or-C
nh
2n+1, n>=0; Described compound structure (I) at least contains the R of Yi Ge functional group, and position and number indefinite;
X is the group of ortho position on phenyl ring, a position, para-position, its architectural feature be functional group-Cl or-SO
3h; Described compound structure (I) at least contains the X of Yi Ge functional group, and position and number indefinite.
Preferably, described brominated industrial bactericide is selected from bromo nitrobenzene ethylene, benzyl acetate bromide, the bromo-5-of 5-nitro-1,3-dioxanes, 2, the bromo-2-nitroethyl alcohol of 2-bis-(DBNE), 1, two (bromine ethyl ketone the oxygen)-2-butylene (BBAB), 1 of 4-, one or more in two (the acetyl bromide oxygen base) ethane (BBAE) of 2-; Preferred, described brominated industrial bactericide is selected from one or both in the bromo-2-nitroethyl alcohol of 2,2-bis-(DBNE) and benzyl acetate bromide.
Preferably, described pyrithione salt is selected from one or more in sodium pyrithione (SPT), pyrithione urea (PM), copper pyrithione (CPT), Zinc Pyrithione (ZPT), pyrithione ferrum, pyrithione, pyrithione ethanolamine salt (OCT); Preferred, described pyrithione salt is Zinc Pyrithione (ZPT).
Preferably, described iodo propinyl compound is selected from one or both in IPBC (IPBC) and iodo propinyl propyl carbamate; Preferred, described iodo propinyl compound is IPBC (IPBC).
Preferably, the described phenol analog derivative that contains compound structure (I) is selected from parabens and salt thereof, sulfosalicylic acid, 2, one or more in the chloro-MX of 4-bis-(DCMX), CDMP (PCMX), triclosan; Preferred, the described phenol analog derivative that contains compound structure (I) is sulfosalicylic acid.Described parabens and salt thereof can be propylparaben, butoben or nipabutyl sodium etc.
Preferably, the described compound that contains aldehyde radical is citral.
Preferably, described pharmaceutical pack is containing Radix Rehmanniae Preparata and/or Radix Sanguisorbae, with following be combined as better: the combination of Radix Rehmanniae Preparata 5-10 part, Radix Sanguisorbae 10-20 part by weight; This combination also can further comprise Folium Isatidis 5-10 part and/or Herba Artemisiae Annuae 5-10 part and/or Pericarpium Granati 10-20 part; It is best take by weight Radix Rehmanniae Preparata 5-10 part, Radix Sanguisorbae 10-20 part, Folium Isatidis 5-10 part, Herba Artemisiae Annuae 5-10 part, Pericarpium Granati 10-20 part.
For realizing above-mentioned second object, the technical scheme that the present invention takes is:
The application of any medicine as above in control saprolegniasis of aquatic animals.
The invention has the advantages that:
1, the invention provides for preventing and treating medicine and the combination thereof of the Fish fish molds being caused by filamentous fungis such as Saprolegnia, Achylas, evidence these medicines and compositions fish molds bacterial strain is had to good prevention effect, test has obtained each medicine and compositions for the minimal inhibitory concentration of fish molds bacterial strain, for the control of fish molds in aquaculture provides important reference frame;
2, compound provided by the invention and composition for preventing and controlling fish molds possess the advantage that minimal inhibitory concentration is low, can reduce dramatically the impact of medicine on food safety, economy, safety;
3, the present invention also provides with Chinese herbal medicine and has prevented and treated fish molds, and the method possesses advantage pollution-free, without any side effects, is particularly suitable for the control of the famous and precious saprolegniasis of aquatic animals such as fancy fishes.
The specific embodiment
Below the specific embodiment provided by the invention is elaborated.
Herein, various compounds provide by Shanghai Pesticide Research Institute; Each herbal raw material medicine is all purchased from chemical reagent company limited of traditional Chinese medicines group (Shanghai).
embodiment 1 compositions one
Bromo nitrobenzene ethylene, SPT, IPBC evenly mix according to weight ratio 1:1:1.
embodiment 2 compositionss two
DBNE, ZPT, pyrithione, nipabutyl sodium evenly mix according to weight ratio 1:1:1:2.
embodiment 3 compositionss three
CPT, iodo propinyl propyl carbamate, DCMX, citral evenly mix according to weight ratio 5:5:1:1.
embodiment 4 compositionss four
BBAB, BBAE, butoben, sulfosalicylic acid evenly mix according to weight ratio 5:5:1:1.
embodiment 5 compositionss five
Benzyl acetate bromide, pyrithione ferrum evenly mix according to weight ratio 4:6.
embodiment 6 compositionss six
OCT, OBPA, IPBC evenly mix according to weight ratio 10:3:1.
embodiment 7 compositionss seven
DBNE, SPT, iodo propinyl propyl carbamate, propylparaben, citral evenly mix according to weight ratio 10:2:2:1:1.
embodiment 8 compositionss eight
Get by weight 5 parts of Radix Rehmanniae Preparata, 20 parts of Radix Sanguisorbaes, pulverize, evenly mix.
embodiment 9 compositionss nine
Get by weight 10 parts of Radix Rehmanniae Preparata, 10 parts of Radix Sanguisorbaes, pulverize, evenly mix.
embodiment 10 compositionss ten
Get by weight 8 parts of Radix Rehmanniae Preparata, 15 parts of Radix Sanguisorbaes, pulverize, evenly mix.
embodiment 11 compositionss 11
Get by weight 5 parts of Radix Rehmanniae Preparata, 20 parts of Radix Sanguisorbaes, 5 parts of Folium Isatidiss, pulverize, evenly mix.
embodiment 12 compositionss 12
Get by weight 10 parts of Radix Rehmanniae Preparata, 10 parts of Radix Sanguisorbaes, 10 parts of Folium Isatidiss, pulverize, evenly mix.
embodiment 13 compositionss 13
Get by weight 5 parts of Radix Rehmanniae Preparata, 20 parts of Radix Sanguisorbaes, 5 parts of Folium Isatidiss, 5 parts of Herba Artemisiae Annuaes, pulverize, evenly mix.
embodiment 14 compositionss 14
Get by weight 10 parts of Radix Rehmanniae Preparata, 10 parts of Radix Sanguisorbaes, 10 parts of Folium Isatidiss, 10 parts of Herba Artemisiae Annuaes, pulverize, evenly mix.
embodiment 15 compositionss 15
Get by weight 8 parts of Radix Rehmanniae Preparata, 15 parts of Radix Sanguisorbaes, 8 parts of Folium Isatidiss, 8 parts of Herba Artemisiae Annuaes, pulverize, evenly mix.
embodiment 16 compositionss 16
Get by weight 5 parts of Radix Rehmanniae Preparata, 20 parts of Radix Sanguisorbaes, 5 parts of Folium Isatidiss, 5 parts of Herba Artemisiae Annuaes, 20 parts of Pericarpium Granatis, pulverize, evenly mix.
embodiment 17 compositionss 17
Get by weight 10 parts of Radix Rehmanniae Preparata, 10 parts of Radix Sanguisorbaes, 10 parts of Folium Isatidiss, 10 parts of Herba Artemisiae Annuaes, 10 parts of Pericarpium Granatis, pulverize, evenly mix.
embodiment 18 compositionss 18
Get by weight 8 parts of Radix Rehmanniae Preparata, 15 parts of Radix Sanguisorbaes, 8 parts of Folium Isatidiss, 8 parts of Herba Artemisiae Annuaes, 15 parts of Pericarpium Granatis, pulverize, evenly mix.
the test of embodiment 19 medical treatment Fish of the present invention fish molds
1 experiment material
Parasitic fish molds ATCC200013
tM, be purchased from the biological product of Unite States Standard collecting center; Different silk floss is mould, and GenBank accession number: JN400035 is preserved in preservation center, Shanghai Ocean University country aquatic animal cause of disease storehouse.
Each herbal raw material medicine Radix Rehmanniae Preparata, Radix Sanguisorbae, Folium Isatidis, Herba Artemisiae Annuae, Pericarpium Granati, and the herbal composite of embodiment 8-18 is prepared its extracting solution: Qiu Qinglian according to the method for following document respectively, Pan Qingqing, Zhang Youping, Deng. the bacteriostasis research [J] of 45 kinds of Chinese herbal medicine extracts such as Galla Chinensis, Fructus Mume to Scylla serrata (Forskal). morbid vibrio. Oceanography Institute Of Zhejiang's journal: natural science edition, 2010,29 (1): 34-38.Concrete extracting method is as follows: get and pulverize herbal raw material medicine 5g, add water to 75ml, 4 ℃ are soaked after 12h, boil the rear slow fire boiling 30min that uses, and leach decocting liquid, then the decocting that adds same volume boils and closes.And 3 decoction liquor, heating is concentrated into 7ml, and the centrifugal 10min of 12000r/min, gets 4 ℃ of supernatants and saves backup.
2 experimental techniques
The following document of reference: 1) Benger S, Townsend P, Ashford RL, et al. An in vitro study to determine the minimum inhibitory concentration of Melaleuca alternifolia against the dermatophyte Trichophyton rubrum[J]. The Foot, 2004,14 (2): 86-91; 2) Stueland S, Heier BT, Skaar I. A simple in vitro screening method to determine the effects of drugs against growth of Saprolegnia parasitica[J]. Mycological Progress, 2005,4 (4): 273-279, according to the coubling dilution in document, measure each medicine to causing a disease fish molds bacterial strain: the external minimal inhibitory concentration (minimum inhibitory concentration, MIC) that parasitic fish molds and different silk floss are mould.Concrete grammar is as follows: the fish molds bacterial strain that will cause a disease is inoculated in PDA culture medium, in incubator, cultivates after 24h for 20 ℃, and the Semen Brassicae campestris of sterilizing is placed in to colony edge, continues at 20 ℃ of cultivation 24h in incubator.Semen Allii Tuberosi with mycelia is taken out, be placed in 24 orifice plates, every Kong Fangyi grain Semen Brassicae campestris, then adds the variable concentrations medicine of 1ml, adds the distilled water of 1ml to contrast simultaneously, each process 3 parallel; Then being placed in 20 ℃ of incubators cultivates after 24h, under stereomicroscope, observe mycelial growth situation, the concentration interpretation that the growth of fish molds mycelia is completely suppressed or relative blank is obviously suppressed, for this medicine is to fish molds mycelia minimal inhibitory concentration (MIC) value, is recorded to drug sensitivity tests.
3 experimental results
Experimental result is in Table 1 and table 2.
Each Chinese herbal medicine of table 1 and the minimal inhibitory concentration of compound to water mo(u)ld
Each compound composition of table 2 and the minimal inhibitory concentration of herbal composite to water mo(u)ld
From table 1 and table 2, medicine of the present invention comprises that each Chinese herbal medicine, each herbal composite, disinfectant, fungusproof anti-corrosion agent, pesticide, various compound compositions etc. all have inhibitory action to parasitic fish molds and different silk floss trichoderma strain, and basically identical to the MIC value of two kinds of bacterial strains.In table, data also can draw, several compounds or Chinese herbal medicine are used in combination to control fish molds, and various medicines can be brought into play synergism, strengthen inhibitory action, thereby can reduce drug dose and obtain more satisfactory effect simultaneously.In addition, clinical trial shows, some medicine of the present invention, if benzyl acetate bromide, DBNE, CPT, ZPT, butoben etc. are when lower than 10 times of minimal inhibitory concentrations, still can be controlled the generation of fish molds preferably.
Because aquatic animal is of a great variety, and different fishes is different to the sensitivity of different pharmaceutical with fish roe, so when the medical treatment fish molds that adopts patent of the present invention to relate to, can select aquatic animal safety, should not cause responsive medicine or compositions use.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, do not departing under the prerequisite of the inventive method; can also make some improvement and supplement, these improvement and supplement and also should be considered as protection scope of the present invention.
Claims (2)
1. a medicine of preventing and treating Fish fish molds, is characterized in that, described medicine is that DBNE, SPT, iodo propinyl propyl carbamate, propylparaben, citral are according to the mixture of weight ratio 10:2:2:1:1.
2. the application of medicine claimed in claim 1 in the medicine of preparation control saprolegniasis of aquatic animals.
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| CN104606188B (en) * | 2015-02-06 | 2018-04-27 | 武汉中博水产生物技术有限公司 | A kind of bactericidal composition and its application in prevention aquaculture disease medicament is prepared |
| CN105461588A (en) * | 2015-12-17 | 2016-04-06 | 上海海洋大学 | Method for compounding metabolites 6 in ridomil |
| CN105616594A (en) * | 2016-03-22 | 2016-06-01 | 五河县金塘水产养殖有限公司金贸园分公司 | Medicine for controlling skin rot disease of blackfish |
| CN106581207A (en) * | 2017-03-06 | 2017-04-26 | 长沙瑞多康生物科技有限公司 | Composition for preventing and controlling fish Saprolegniasis |
| CN106943389B (en) * | 2017-04-07 | 2019-06-21 | 上海海洋大学 | It is a kind of prevent and treat fish-egg saprolegniasis preparation and its application |
| CN106963758A (en) * | 2017-04-07 | 2017-07-21 | 上海海洋大学 | A kind of preparation for preventing and treating fish-egg saprolegniasis and its application |
| CN107519432A (en) * | 2017-07-20 | 2017-12-29 | 通化师范学院 | One kind prevents and treated to add body and purposes in Salvelinus malma saprolegniasis water |
| CN107711631B (en) * | 2017-11-22 | 2020-12-29 | 贵州渔恒丰农业科技有限公司 | Method for preventing and treating fish skin ulceration |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1121413A (en) * | 1994-07-29 | 1996-05-01 | 索玛株式会社 | Antimicrobial composition |
| JPH09278717A (en) * | 1996-04-15 | 1997-10-28 | Kurita Water Ind Ltd | Clathrate compound |
| CN102783482A (en) * | 2011-05-19 | 2012-11-21 | 大连百傲化学有限公司 | 3-isothiazolinone composition |
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2013
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1121413A (en) * | 1994-07-29 | 1996-05-01 | 索玛株式会社 | Antimicrobial composition |
| JPH09278717A (en) * | 1996-04-15 | 1997-10-28 | Kurita Water Ind Ltd | Clathrate compound |
| CN102783482A (en) * | 2011-05-19 | 2012-11-21 | 大连百傲化学有限公司 | 3-isothiazolinone composition |
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