Summary of the invention
In view of this, the object of the invention is to provide a kind of biological Pesticidal foliage fertilizer of disease-resistant yield-increasing, makes biological Pesticidal foliage fertilizer of the present invention can improve the particularly effect of the disease-resistant yield-increasing of vegetables of farm crop.
For realizing purpose of the present invention, the present invention adopts following technical scheme:
A kind of biological Pesticidal foliage fertilizer, be calculated in mass percent, by fungal organism albumen 0.2%-3%, macroelement fertilizer, wetting agent 0.5%-1%, dispersion agent 0.3%-0.5%, thickening material 0.3%-0.5%, surplus is that filler or water form, and described macroelement fertilizer is for comprising at least in macroelement nitrogen, phosphorus, potassium the fertilizer of two kinds, and in macroelement fertilizer, the macroelement total amount is not less than 50% of biological Pesticidal foliage fertilizer total amount;
Wherein, described fungal organism albumen is prepared by following methods:
with Staphlosporonites, interlink spore genus, rice blast fungus, the poison mould, Aspergillus flavus, any one in sheath blight fungus and Penicillium notatum is material, when adding nutrient solution to be cultured to fuugal spores counts no longer to increase, through 5000rpm centrifugal 30 minutes, collect spore and add the 5mM potassium phosphate buffer of pH6.8 or citrate buffer solution to obtain fungal spore suspension by 1/10 of nutrient solution volume, after 180bar high pressure or 30 minutes ultrasonic disruption spore cells, 5000rpm is centrifugal 30 minutes again, get supernatant liquor and use boiling water water-bath 15 minutes, then 5000rpm is centrifugal 30 minutes, the gained supernatant liquor is fungal organism albumen.
As preferably, each macroelement content in described macroelement fertilizer is not less than 4%, described macroelement fertilizer is selected from two or more in ammonium sulfate, ammonium chloride, primary ammonium phosphate, urea, potassium primary phosphate, potassium hydrogen phosphate, Repone K, vitriolate of tartar and Tripotassium Citrate, the macroelement fertilizer that described macroelement fertilizer more preferably is comprised of potassium primary phosphate and urea or the macroelement fertilizer that is comprised of primary ammonium phosphate and Repone K.
In the preparation process of fungal organism albumen, as preferably, described material is Aspergillus flavus.
Other components for biological Pesticidal foliage fertilizer of the present invention, as preferably, described wetting agent is one or more in the mixture of sodium lauryl sulphate, sodium laurylsulfonate, sodium alkyl naphthalene sulfonate, fatty alcohol polyglycol ether vitriol, fat alcohol polyethylene ether, Nekal BX, moistening penetrant t/polyoxyethylene nonylphenol ether, sorbitan fatty acid ester Soxylat A 25-7, alkylnaphthalene formaldehyde condensation products, sulfonated alkyl naphathalene and anionic wetting agent;
Preferably, described dispersion agent is one or more in NNO, sodium lignosulfonate, calcium lignin sulphonate, sodium polyacrylate, methylene-bis naphthalenesulfonate, alkyl amine group taurate, sodium methylene bis-naphthalene sulfonate formaldehyde condensation products, polyoxyethylene polyoxypropylene block copolymer, polycarboxylate, carboxymethyl cellulose, tripoly phosphate sodium STPP, sulfonated alkyl naphathalene polycondensate.
Preferably, described thickening material is one or more in xanthan gum, polyvinyl alcohol, sodium alginate, starch, organobentonite.
Preferably, described filler is one or more in kaolin, diatomite, light calcium carbonate, Attapulgite, wilkinite, white carbon black.
Fungal organism albumen of the present invention (Activator Protein) is novel protein agricultural chemicals that a class is separated from the Various Diseases fungal pathogens, that have the albumen arousal function, can induce various plants to produce system's resistance, Promoting plant growth improves crop quality.Study on mechanism shows, plant activation protein improves plant autoimmunity by activated plant vivo immuning system and metabolic regulation system, promotes the growth of roots of plants cauline leaf, thereby strengthens the plant resistance against diseases, improves crop yield.Therefore plant activation protein without direct killing action, can not cause that the cause of disease physiological strain produces resistance to target pathogens,, noresidue safety low-poison to environment, people and animals.
The present invention select macroelement fertilizer and fungal organism albumen composite, detect through manure trial, between the two without impact, use under the prerequisite of identical activeconstituents consumption guaranteeing, compare with output with the existing preventive effect of using separately macroelement fertilizer and fungal organism albumen and be significantly improved.This shows, this synergism of biological medicine fertilizer of the present invention is not that the direct effect due to another kind of active substance produces, but both are mutually collaborative, promote the effect synergy of automatic sterilization function volume increase.
Sterilization disease-resistant composition of the present invention can improve the effect, particularly vegetables of disease-resistant crops volume increase, as cucumber, tomato, Plantula Brassicae chinensis etc.Described vegetable disease is preferably oidium, gray mold and anthrax.
By above technical scheme as can be known, the present invention is composite as effective constituent with fungal organism albumen and nitrogen phosphorus potassium macroelement fertilizer, both have complementary advantages, applied once not only can improve the preventive effect of corps diseases, can also improve crop yield, reduced the usage quantity of agricultural chemicals and chemical fertilizer and used kind, number of times, can be widely used in agriculture field.
Embodiment
The embodiment of the invention discloses a kind of biological Pesticidal foliage fertilizer.Those skilled in the art can use for reference this paper content, suitably improve processing parameter and realize.Special needs to be pointed out is, all similarly replace and change apparent to those skilled in the art, and they all are deemed to be included in the present invention.Product of the present invention and method are described by preferred embodiment, and the related personnel obviously can change product as herein described within not breaking away from content of the present invention, spirit and scope or suitably change and combination, realizes and use the technology of the present invention.
In embodiments of the present invention, described fungal organism albumen is Aspergillus flavus, Penicillium notatum, sheath blight fungus, Aspergillus flavus, rice blast fungus according to the material that the embodiment sequence number adopts successively, and the preparation method is referring to summary of the invention.
Further set forth the present invention below in conjunction with embodiment.
Embodiment 1: biological Pesticidal foliage fertilizer (fungal organism albumen 0.3%, N=18.0%, P of the present invention
2O
5=19.3%, K
2O=12.7%)
Fungal organism albumen 0.3%, potassium primary phosphate 37%, urea 38.6%, sodium laurylsulfonate 0.8%, calcium lignin sulphonate 0.4%, yellow collagen 0.1%, kaolin supply 100%.
With fungal organism albumen, sodium laurylsulfonate, the abundant mix and blend of calcium lignin sulphonate, then add potassium primary phosphate, urea, yellow collagen, kaolin namely to obtain product through fully mixing, pulverizing.
Verify for the disease-resistant yield-increasing effect to the present embodiment product, carry out following test.
Process 1: the present embodiment product, be diluted to 800 times of liquid, from Plantula Brassicae chinensis seedling stage, spray in 7 days is once sprayed 3 times altogether.
Process 2: fungal organism albumen, and process in 1 identically, from Plantula Brassicae chinensis seedling stage, spray in 7 days is once sprayed 3 times altogether, and total usage quantity is identical with processing 1.
Process 3: potassium primary phosphate and urea, and process in 1 identically, from Plantula Brassicae chinensis seedling stage, spray in 7 days is once sprayed 3 times altogether, and total usage quantity is identical with processing 1.
Test-results sees Table 1.
The impact of the biological Pesticidal foliage fertilizer of table 1 on yield of pakchoi and disease resistance ability
Process |
Increase of production rate/% |
Plantula Brassicae chinensis oidium sickness rate/% |
Plantula Brassicae chinensis anthrax sickness rate % |
The present embodiment product |
10 |
20.5 |
18.5 |
Fungal organism albumen |
6 |
43.2 |
32.6 |
Potassium primary phosphate, urea |
5.5 |
53.4 |
43.6 |
Clear water |
0 |
65.7 |
53.5 |
As shown in Table 1, the Plantula Brassicae chinensis of processing through the present embodiment Pesticidal foliage fertilizer increases than the yield of pakchoi of processing with macroelement fertilizer and fungal organism albumen separately, and has reduced the sickness rate of Plantula Brassicae chinensis oidium and anthrax.Under the prerequisite identical with the macroelement fertilizer usage quantity with independent use fungal organism albumen, preventive effect and the output of biological foliage fertilizer material of the present invention significantly improve.This shows the not simple addition of effect of this raising disease-resistant yield-increasing of biological Pesticidal foliage fertilizer of the present invention and producing, but both collaborative effects mutually.
Embodiment 2: biological Pesticidal foliage fertilizer (fungal organism albumen 0.5%, N=6.0%, P of the present invention
2O
5=30.6%, K
2O=20.8%)
Fungal organism albumen 0.5%, primary ammonium phosphate 50%, Repone K 33%, sodium lauryl sulphate 0.6%, carboxymethyl cellulose 0.3%, polyvinyl alcohol 0.4%, diatomite supply 100%.
With fungal organism albumen, sodium lauryl sulphate, the abundant mix and blend of carboxymethyl cellulose, then add primary ammonium phosphate, Repone K, polyvinyl alcohol, diatomite through fully mix, pulverize, dry, sieving namely obtains product.
Verify for the disease-resistant yield-increasing effect to the present embodiment product, carry out following test.
Process 1: the present embodiment product, be diluted to 1000 times of liquid, from Plantula Brassicae chinensis seedling stage, spray in 7 days is once sprayed 3 times altogether.
Process 2: fungal organism albumen, and process in 1 identically, from Plantula Brassicae chinensis seedling stage, spray in 7 days is once sprayed 3 times altogether, and total usage quantity is identical with processing 1.
Process 3: primary ammonium phosphate and Repone K, and process in 1 identically, from Plantula Brassicae chinensis seedling stage, spray in 7 days is once sprayed 3 times altogether, and total usage quantity is identical with processing 1.
Test-results sees Table 2.
The impact of the biological Pesticidal foliage fertilizer of table 2 on cucumber yield and disease resistance ability
Process |
Increase of production rate/% |
Cucumber downy mildew sickness rate/% |
Cucumber anthracnose sickness rate % |
The present embodiment product |
10 |
13.5 |
15.2 |
Fungal organism albumen |
7 |
35.2 |
41.5 |
Primary ammonium phosphate, Repone K |
5.5 |
44.5 |
53.4 |
Clear water |
0 |
57.8 |
63.6 |
As shown in Table 2, the cucumber of processing through the present embodiment Pesticidal foliage fertilizer increases than the cucumber yield of processing with macroelement fertilizer and fungal organism albumen separately, and has reduced the sickness rate of cucumber downy mildew and anthrax.Under the prerequisite identical with the macroelement fertilizer usage quantity with independent use fungal organism albumen, preventive effect and the output of biological foliage fertilizer material of the present invention significantly improve.This shows the not simple addition of effect of this raising disease-resistant yield-increasing of biological Pesticidal foliage fertilizer of the present invention and producing, but both collaborative effects mutually.
Embodiment 3: biological Pesticidal foliage fertilizer (fungal organism albumen 0.3%, N=18%, P of the present invention
2O
5=19.3%, K
2O=12.7%)
Fungal organism albumen 0.3%, potassium primary phosphate 37%, urea 38.6%, polyoxyethylene nonylphenol ether 0.8%, calcium lignin sulphonate 0.4%, xanthan gum 0.3%, water supply 100%.
With fungal organism albumen, polyoxyethylene nonylphenol ether, calcium lignin sulphonate mix and blend, then add primary ammonium phosphate, Repone K, xanthan gum, add water and namely obtain product through fully mixing, dissolving.
Verify for the disease-resistant yield-increasing effect to the present embodiment product, carry out following test.
Process 1: the present embodiment product, be diluted to 1000 times of liquid, from Plantula Brassicae chinensis seedling stage, spray in 7 days is once sprayed 3 times altogether.
Process 2: fungal organism albumen, and process in 1 identically, from Plantula Brassicae chinensis seedling stage, spray in 7 days is once sprayed 3 times altogether, and total usage quantity is identical with processing 1.
Process 3: primary ammonium phosphate and Repone K, and process in 1 identically, from Plantula Brassicae chinensis seedling stage, spray in 7 days is once sprayed 3 times altogether, and total usage quantity is identical with processing 1.
Test-results sees Table 3.
The impact of the biological Pesticidal foliage fertilizer of table 3 on tomato yield and disease resistance ability
Process |
Increase of production rate/% |
Graw mold of tomato sickness rate/% |
Anthracnose of tomato sickness rate % |
The present embodiment product |
18 |
10.2 |
8.3 |
Fungal organism albumen |
6 |
15.6 |
18.5 |
Primary ammonium phosphate, Repone K |
5 |
24.7 |
31.4 |
Clear water |
0 |
54.3 |
56.7 |
As shown in Table 3, the tomato of processing through the present embodiment Pesticidal foliage fertilizer increases than the tomato yield of processing with macroelement fertilizer and fungal organism albumen separately, and has reduced the sickness rate of graw mold of tomato and anthrax.Under the prerequisite identical with the macroelement fertilizer usage quantity with independent use fungal organism albumen, preventive effect and the output of biological foliage fertilizer material of the present invention significantly improve.This shows the not simple addition of effect of this raising disease-resistant yield-increasing of biological Pesticidal foliage fertilizer of the present invention and producing, but both collaborative effects mutually.
Embodiment 4: biological Pesticidal foliage fertilizer (fungal organism albumen 0.5%, N=6%, P of the present invention
2O
5=30.6%, K
2O=20.8%)
Fungal organism albumen 0.5%, primary ammonium phosphate 50%, Repone K 33%, fat alcohol polyethylene ether 0.6%, carboxymethyl cellulose 0.3%, polyvinyl alcohol 0.4%, water supply 100%.
With fungal organism albumen, fat alcohol polyethylene ether, the abundant mix and blend of carboxymethyl cellulose, then add primary ammonium phosphate, Repone K, polyvinyl alcohol, add water through fully mix, dissolving namely obtains product.
Verify for the disease-resistant yield-increasing effect to the present embodiment product, carry out following test.
Process 1: the present embodiment product, be diluted to 1000 times of liquid, from Plantula Brassicae chinensis seedling stage, spray in 7 days is once sprayed 3 times altogether.
Process 2: fungal organism albumen, and process in 1 identically, from Plantula Brassicae chinensis seedling stage, spray in 7 days is once sprayed 3 times altogether, and total usage quantity is identical with processing 1.
Process 3: primary ammonium phosphate and Repone K, and process in 1 identically, from Plantula Brassicae chinensis seedling stage, spray in 7 days is once sprayed 3 times altogether, and total usage quantity is identical with processing 1.
Test-results sees Table 4.
The impact of the biological Pesticidal foliage fertilizer of table 4 on tomato yield and disease resistance ability
Process |
Increase of production rate/% |
Graw mold of tomato sickness rate/% |
Anthracnose of tomato sickness rate % |
The present embodiment product |
20 |
9.5 |
11.5 |
Fungal organism albumen |
10 |
18.2 |
26.2 |
Primary ammonium phosphate, Repone K |
6 |
20.5 |
31.4 |
Clear water |
0 |
23.5 |
38.5 |
As shown in Table 4, the tomato of processing through the present embodiment Pesticidal foliage fertilizer increases than the tomato yield of processing with macroelement fertilizer and fungal organism albumen separately, and has reduced the sickness rate of graw mold of tomato and anthrax.Under the prerequisite identical with the macroelement fertilizer usage quantity with independent use fungal organism albumen, preventive effect and the output of biological foliage fertilizer material of the present invention significantly improve.This shows the not simple addition of effect of this raising disease-resistant yield-increasing of biological Pesticidal foliage fertilizer of the present invention and producing, but both collaborative effects mutually.
Embodiment 5: biological Pesticidal foliage fertilizer (fungal organism albumen 0.9%, N=9.6%, P of the present invention
2O
5=19.7%, K
2O=25.6%)
Fungal organism albumen 0.9%, ammonium chloride 37%, potassium primary phosphate 38%, vitriolate of tartar 25%, Nekal BX 0.6%, calcium lignin sulphonate 0.5%, sodium alginate 0.3%, kaolin supply 100%.
With fungal organism albumen, sorbitan fatty acid ester Soxylat A 25-7, the abundant mix and blend of tripoly phosphate sodium STPP, then add ammonium chloride, potassium primary phosphate, vitriolate of tartar, kaolin through fully mix, pulverize, dry, sieving namely obtains product.
Verify for the disease-resistant yield-increasing effect to the present embodiment product, carry out following test.
Process 1: the present embodiment product, be diluted to 1000 times of liquid, from Plantula Brassicae chinensis seedling stage, spray in 7 days is once sprayed 3 times altogether.
Process 2: fungal organism albumen, and process in 1 identically, from Plantula Brassicae chinensis seedling stage, spray in 7 days is once sprayed 3 times altogether, and total usage quantity is identical with processing 1.
Process 3: primary ammonium phosphate and Repone K, and process in 1 identically, from Plantula Brassicae chinensis seedling stage, spray in 7 days is once sprayed 3 times altogether, and total usage quantity is identical with processing 1.
Test-results sees Table 5.
The impact of the biological Pesticidal foliage fertilizer of table 5 on tomato yield and disease resistance ability
Process |
Increase of production rate/% |
Graw mold of tomato sickness rate/% |
Anthracnose of tomato sickness rate % |
The present embodiment product |
18 |
12.5 |
5.4 |
Fungal organism albumen |
11 |
23.8 |
21.4 |
Primary ammonium phosphate, Repone K |
8 |
36.5 |
28.7 |
Clear water |
0 |
41.2 |
35.6 |
As shown in Table 5, the tomato of processing through the present embodiment Pesticidal foliage fertilizer increases than the tomato yield of processing with macroelement fertilizer and fungal organism albumen separately, and has reduced the sickness rate of graw mold of tomato and anthrax.Under the prerequisite identical with the macroelement fertilizer usage quantity with independent use fungal organism albumen, preventive effect and the output of biological foliage fertilizer material of the present invention significantly improve.This shows the not simple addition of effect of this raising disease-resistant yield-increasing of biological Pesticidal foliage fertilizer of the present invention and producing, but both collaborative effects mutually.
The explanation of above embodiment just is used for helping to understand method of the present invention and core concept thereof.Should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the principle of the invention, can also carry out some improvement and modification to the present invention, these improvement and modification also fall in the protection domain of claim of the present invention.