CN103055011A - G6 lectin extracted from traditional Chinese medicines and applications thereof in detoxification, pressure stabilization, blood vessel softening and complication resistance - Google Patents
G6 lectin extracted from traditional Chinese medicines and applications thereof in detoxification, pressure stabilization, blood vessel softening and complication resistance Download PDFInfo
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- CN103055011A CN103055011A CN 201110321989 CN201110321989A CN103055011A CN 103055011 A CN103055011 A CN 103055011A CN 201110321989 CN201110321989 CN 201110321989 CN 201110321989 A CN201110321989 A CN 201110321989A CN 103055011 A CN103055011 A CN 103055011A
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Abstract
The invention discloses a G6 lectin extracted from the traditional Chinese medicines, namely pseudo-ginseng, eucommia ulmoides, radix puerariae, rehmannia, salvia miltiorrhiza and kelp. A preparation method of the lectin comprises the following steps of: 1) weighing dry pseudo-ginseng, eucommia ulmoides, radix puerariae, rehmannia, salvia miltiorrhiza and kelp according to the same weight, grinding thetraditional Chinese medicines into mixed medicine powder with the granularity of 50-100 meshes; 2) adding the obtained mixed medicine powder into a Tris buffer solution of which the volume is 5 times that of the mixed medicine powder, homogenizing, soaking for 12 hours at the temperature of 4DEG C, and centrifuging to obtain a supernatant; 3) filtering the obtained supernatant by a hypertonic agglutination method to remove impurities to obtain phytolectin-containing filter liquor; and 4) dialyzing, concentrating and drying the filter liquor to obtain the G6 lectin. The G6 lectin provided by the invention not only has the characteristics of the phytolectin, but also has an adjusting function for recovering a human sympathetic nervous system, so that the G6 lectin disclosed by the invention has a bran-new breakthrough for the stabilization of the hypertension and the improvement of the complication.
Description
Technical field
The present invention relates to the field of Chinese medicines, particularly a kind of G6 agglutinin of Chinese medicine extraction and in toxin expelling voltage stabilizing, vessel softening, purposes in anti-concurrent.
Background technology
Agglutinin (lectin) is glycoprotein or the sugared albumen of combination that zooblast and plant cell can both synthesize and secrete, because of its energy coagulation erythrocyte (containing blood group substance), therefore named agglutinin.That commonly used is phytohemagglutinin (Phytoagglutin, PNA), usually with its plant name that is extracted, such as concanavalin A (Conconvalina, ConA), Fructus Hordei Germinatus element (Wheat germ agglutinin, WGA), peanut agglatinin (Peanut agglutinin, PNA) and soybean agglutinin (Soybean agglutinin, SBA) etc., agglutinin is their general name.Agglutinin plays an important role in cell recognition and adhesion reaction, mainly is to promote intercellular adhesion.The characteristics of agglutinin maximum are that they can identify in glycoprotein and the glycopeptide, particularly complicated carbohydrate structure in the cell membrane, the i.e. esterified compound determinant of the carbon of surface of cell membrane.A kind of agglutinin has the ability to the combination of a certain specificity glycosyl specificity, and therefore, agglutinin can be used as a kind of probe and studies specific glycosyl on the cell membrane.On the other hand, agglutinin has the multivalence binding ability, can be combined with tracers such as fluorescein, biotin, enzyme, gold colloidal and ferritins, thereby show its binding site at light microscopic and Electronic Speculum level.
Agglutinin not only has the effects such as coagulation such as hemocyte, lymphocyte, spermatid, and some important physiology and pharmacology process in the organism have been participated in, therefore be the exceedingly useful instruments of life process such as the canceration of postgraduate's object inner cell, fertilization, differentiation and molecular recognition, simultaneously at clinical medical inspection critical role is arranged, and be that the required mitogenesis of antitumor drug production is former.Report also that in recent years some agglutinin also has inhibitory action to HIV (human immunodeficiency virus) etc., and be applied to experimental treatment.But up to the present, yet there are no the research report that agglutinin acts in hypertension and other cardiovascular and cerebrovascular diseases.
Although the varied Chinese medicine that comprises of domestic existing hypertension therapeutic mode, Western medicine, physiotherapeutic instruments, but every kind of mode has its drawback regrettably, the Western medicine blood pressure lowering is quick but voltage regulation result is bad, toxic and side effects is huge, can not stop the generation of hypertension complication, and China has at present near 200,000,000 Hypertensive Population, and the crowd who takes Western medicine accounts for 3/4ths of all Hypertensive Population, Most patients is known side effect and the drawback of Western medicine, can in the optional situation without medicine, only have the selection Western medicine; And Chinese medicine is taken inconvenience, and drug effect runs off serious; Physiotherapy table can only be alleviated for the moment symptom.Therefore, find a kind of steadily blood pressure lowering, the biomolecule that can reduce again the toxic and side effects that western medicine of depressurization brings has become the task of top priority.
Summary of the invention
The technical problem to be solved in the present invention can't be got rid of for taking the western medicine of depressurization toxic and side effects for hyperpietic in the prior art, and the problem that easily has complication to occur provides a kind of Chinese medicine extraction G6 agglutinin that has toxin expelling voltage stabilizing, logical blood vessel, prevents the complication effect.
For solving the problems of the technologies described above, G6 agglutinin provided by the invention is: obtain by extraction from Chinese medicine Radix Notoginseng, the Cortex Eucommiae, Radix Puerariae, Radix Rehmanniae, Radix Salviae Miltiorrhizae, Thallus Laminariae (Thallus Eckloniae), and contain Radix Salviae Miltiorrhizae acid, Radix Salviae Miltiorrhizae flavone, arasaponin, Radix Rehmanniae saponin, rehmanin, laminaran, Radix Puerariae flavone, Cortex Eucommiae terpene component.
The preparation scheme of this agglutinin is:
Step 1) takes by weighing Radix Notoginseng, the Cortex Eucommiae, Radix Puerariae, Radix Rehmanniae, Radix Salviae Miltiorrhizae, the dried medicine of Thallus Laminariae (Thallus Eckloniae) by equal in quality, be ground into 50～100 order medicament mixed powder;
Step 2) medicament mixed powder obtained above is added the Tris buffer of 5 times of volumes, homogenate, 4 ℃ of lower immersions 12 hours, centrifugal, get supernatant;
Step 3) supernatant obtained above is utilized high method of oozing coagulation filter, remove impurity, obtain containing the filtrate of phytohemagglutinin;
Step 4) with above-mentioned filtrate dialysis, concentrated, dry, obtains the G6 agglutinin.
G6 agglutinin provided by the invention is as the basis take motherland's traditional Chinese medical science tradition prescription, select hypertension, cardiovascular and cerebrovascular vessel have six kinds of raw material of Chinese medicine of Radix Notoginseng, the Cortex Eucommiae, Radix Puerariae, Radix Rehmanniae, Radix Salviae Miltiorrhizae, Thallus Laminariae (Thallus Eckloniae) of fine therapeutical effect to extract exclusive G6 agglutinin, the characteristic that not only has phytohemagglutinin, have simultaneously the regulatory function of recovering the human body sympathetic nervous system, in the improvement of stablizing hypertension and complication thereof brand-new breakthrough is arranged.
Wherein Radix Notoginseng has another name called Radix Notoginseng, is the distinctive rare Chinese medicine of China.Put down in writing in Qing Dynasty's pharmacy works supplementary Amplifications of the Compendium of Materia Medica: " ginseng qi-tonifying the first, Radix Notoginseng enrich blood the first, flavor with and merit also waits, therefore claim Panax pseudoginseng, be the most precious person in the Chinese medicine." Radix Notoginseng has blood vessel dilating, reduces blood pressure, and improves microcirculation, increases blood flow, the Function and operation of prevention and treatment heart and brain tissues ischemia, anoxia.
The Cortex Eucommiae is the dry bark of the Eucommiaceae plant Cortex Eucommiae, is Chinese famous and precious tonic herb.Tool invigorating the liver and kidney, bone and muscle strengthening, blood pressure lowering, many effects such as antiabortive.Shennong's Herbal is classified as top grade.Call its " cure mainly waist and knee, invigorating middle warmer, beneficial vital essence, hard muscles and bones, wet except itching under cloudy, dribbling urination.Clothes are made light of one's life by commiting suicide anti-old for a long time." Cortex Eucommiae is Chinese peculiar medical material, its medicinal history is long, has a wide range of applications clinical.
Radix Puerariae is legume pueraria lobata, contains multiple flavone component such as isoflavones components puerarin, puerarin xyloside, daidzein, daidzin and cupreol, arachidic acid, contains again volume starch.The famous physician's Li Shizhen (1518-1593 A.D.) of the Ming Dynasty has carried out systematic research to Radix Puerariae, think the stem, leaf, flower of Radix Puerariae, really, root all can be used as medicine.Compendium of Material Medica is carried: Radix Puerariae, cool in nature, gas is put down, sweet in the mouth, tool heat clearing away, pathogenic fire reducing, all effects of toxin expelling.Modern medicine study shows: the isoflavonoid puerarin in the Radix Puerariae has certain curative effect to hypertension, hyperlipidemia, hyperglycemia and cardiovascular and cerebrovascular disease.
Radix Rehmanniae is the Scrophulariaceae herbaceos perennial, and sweet in the mouth, hardship are cold in nature.Chemical composition is take glycoside as main, wherein again take iridoid glycosides as main.Modern medicine experimental results show that, Radix Rehmanniae 70% methanolic extract suppresses the rat platelet aggregation that ADP causes, and the antithrombase effect is arranged, the rats with diffuse intravascular coagulation that induced by endotoxin causes has antagonism, therefore all kinds of cardiovascular and cerebrovascular diseases is had auxiliary therapeutic action.
Radix Salviae Miltiorrhizae has another name called Radix Salviae Miltiorrhizae, Radix Salviae Miltiorrhizae, Arisaema balansae Engl. etc.It mainly contains fat-soluble Diterpenoids from bulbus and water miscible phenolic acid composition, also contains flavonoid, triterpenes, other compositions such as sterol.Modern pharmacology is tested and is shown, Radix Salviae Miltiorrhizae has the myocardial contraction of reinforcement, improves cardiac function, does not increase myocardial oxygen consumption, and coronary artery dilating increases myocardial flow; The expansion peripheral blood vessel, the effect that blood flow is increased, and Radix Salviae Miltiorrhizae can improve microcirculation, improves plasmin activity; Prolong, clotting time; Anticoagulant.Radix Salviae Miltiorrhizae can make aorta atheromatous plaque formation area obviously reduce serum total cholesterol, triglyceride, all decreases to some degree.The blood fat that Radix Salviae Miltiorrhizae can suppress the high lipid diet rabbit rises.Find that by research danshensu can also suppress the synthetic of cell endogenous cholesterol, blood fat reducing.
Thallus Laminariae (Thallus Eckloniae) is thallus or the Alariaceae plant of Laminariaceae plant Thallus Laminariae (Thallus Eckloniae), and its chemical composition contains algin element, mannitol, galactan, laminine, Thallus Laminariae (Thallus Eckloniae) polysaccharide, glutamic acid, aspartic acid, proline, vitamin B1, C, P and sulphur, potassium etc.Thallus Laminariae (Thallus Eckloniae) has blood fat reducing and anticoagulation, and the laminarin in the Thallus Laminariae (Thallus Eckloniae) can suppress significantly the content increase of hyperlipidemia chicken serum T-CHOL, triglyceride, and can reduce formation and the development of chicken aortic tunica intima atheromatous plaque through gavage repeatedly.
G6 agglutinin provided by the invention is from above-mentioned Six-element Chinese medicine, utilizes the modern high biological active matter molecule that agglutination technology extracts that oozes.
At present domestic existing Chinese traditional medicine purification technology is nothing more than water decoction-alcohol sedimentation, filtration, concentrated, several modes such as enzymolysis, and these traditional purifying techniques have more and more exposed problem, as it is huge to expend raw material, and drug effect runs off, and purity can not guarantee etc.
And the employing of the purifying technique of G6 agglutinin is that advanced in the world height oozes agglutination technology, thoroughly avoided the appearance of these problems, height oozes agglutination technology can select composition useful to disease in the Chinese medicine accurately, with its accurate identification, purifies, coagulation out, and the G6 agglutinin that adopts this technology to extract has high permeability, is convenient to absorption of human body, utilize, can greatly improve it to the adjuvant treatment effect of disease.
Height oozes agglutination technology with its efficient, energy-saving and environmental protection and the characteristics such as molecular level separates, and has been widely used in the fields such as medicine, food processing, becomes one of state-of-the-art technology in modern purification technique field.Be acknowledged as 21 century the height of one of great industrial technology ooze agglutination technology, be a kind of emerging green industry science and technology.Compare with methods such as the centrifugalize of routine, sedimentation, filtration, extractions, height oozes the obvious potential advantages that agglutination technology has.Along with people in recent years ooze the Devoting Major Efforts To Developing of agglutination technology to height since, developed suitable hypertension, cardiovascular and cerebrovascular vessel, the treatment product of the various diseases such as tumor.Especially developed for hypertension and had representational G6 agglutinin, overcome Chinese medicine, the drawback such as the Western medicine effect is not comprehensive, and drug effect is single.
The contained chemical composition of Chinese medicine is very complicated, and saccharide, aminoacid, protein, oils and fats, wax, enzyme, pigment, vitamin, organic acid, tannin, inorganic salt, volatile oil, alkaloid, glycoside etc. are arranged usually.Effective ingredient is namely arranged in these compositions, invalid impurity is arranged again, as do not remove impurity as far as possible, can affect quality and the stability of preparation, high ooze agglutination technology then have high identity and selectivity can be with separating the useful complete components of disease wherein.
Height oozes agglutination technology and adopts staged isolated by filtration medicinal liquid, reasonably uses the non-medicinal impurity that waits in the separating for several times method removal medicinal liquid.And isolated medicinal liquid carried out effective purification, macromolecular substances and the microorganisms such as filtering protein, starch, pectin, tannin reduce the loss of aqueous soluble active constituent, make Effective Component of Chinese Medicine substantially without holding back.
Adopt the high agglutination technology of oozing that medicinal liquid is carried out secondary filter to remove the impurity such as tannin, starch, replace one time precipitate with ethanol, reduce the precipitate with ethanol number of times, until produce without alcoholization.Thereby substituted traditional decoction and alcohol sedimentation technique technique, shortened the production cycle, reached medicinal liquid clear and bright and without alcoholization, purity is high, is beneficial to the purpose of absorption of human body.
G6 agglutinin provided by the invention includes plurality of active ingredients, comprising Radix Salviae Miltiorrhizae acid, Radix Salviae Miltiorrhizae flavone, arasaponin, Radix Rehmanniae saponin, rehmanin, laminaran, Radix Puerariae flavone and Cortex Eucommiae terpenes.
In the embodiment of the invention, the inventor has carried out determination of activity to the G6 agglutinin of preparation, and the result shows, the vigor that has the red cell agglutination made from the G6 agglutinin of the inventive method preparation, and be better than the soybean agglutinin standard substance in effect.
In an embodiment of the present invention, the inventor also is studied to the hypotensive effect of G6 agglutinin with to the inhibition of toxic and side effects that depressor brings.The result shows, the G6 agglutinin of the present invention's preparation uses separately has good hypotensive effect; When uniting use with depressor, its antihypertensive effect is better, and there were significant differences with the blank group, and is better than using separately the situation of depressor; When uniting use with depressor, can eliminate the toxic and side effects that depressor brings.
Major advantage of the present invention is as follows:
1) the present invention has extracted the G6 agglutinin from Six-element has the Chinese medicine of blood pressure lowering toxin expelling, logical blood vessel, control hypertension complication, it not only has the characteristic of phytohemagglutinin, take with Western medicine and can also remove the lesions of liver and kidney that Western medicine brings to human body, have simultaneously the regulatory function of recovering the human body sympathetic nervous system, in stabilizing blood pressure, control complication good effect is arranged;
2) height of the present invention oozes agglutination technology and can select accurately composition useful to disease in the Chinese medicine, with its accurate identification, purify, coagulation out, and the G6 agglutinin that adopts this technology to extract has high permeability, is convenient to absorption of human body, utilize, can greatly improve it to the adjuvant treatment effect of disease;
3) G6 agglutinin provided by the invention is the galenical of pure natural because of it, by the integral body of patient body situation is nursed one's health to reach final stabilizing blood pressure, prevent the purpose that cardiovascular and cerebrovascular disease occurs, therefore to hypertension and cardiovascular and cerebrovascular complication to improve effect steady, incremental.
The specific embodiment
The invention discloses a kind of G6 agglutinin of Chinese medicine extraction, those skilled in the art can use for reference this paper content, suitably improve technological parameter and realize.Special needs to be pointed out is, all similarly replace and change apparent to those skilled in the art, and they all are deemed to be included in the present invention.Method of the present invention and application are described by preferred embodiment, the related personnel obviously can change or suitably change and combination methods and applications as herein described within not breaking away from content of the present invention, spirit and scope, realizes and use the technology of the present invention.
Technical scheme provided by the invention is:
Step 1) takes by weighing Radix Notoginseng, the Cortex Eucommiae, Radix Puerariae, Radix Rehmanniae, Radix Salviae Miltiorrhizae, the dried medicine of Thallus Laminariae (Thallus Eckloniae) by equal in quality, be ground into 50～100 order medicament mixed powder;
Step 2) medicament mixed powder obtained above is added the Tris buffer of 5 times of volumes, homogenate, 4 ℃ of lower immersions 12 hours, centrifugal, get supernatant;
Step 3) supernatant obtained above is utilized high method of oozing coagulation filter, remove impurity, obtain containing the filtrate of phytohemagglutinin;
Step 4) with above-mentioned filtrate dialysis, concentrated, dry, obtains the G6 agglutinin.
In order to make those skilled in the art understand better technical scheme of the present invention, the present invention is described in further detail below in conjunction with specific embodiment.
The preparation of embodiment 1:G6 agglutinin
Take by weighing Radix Notoginseng, the Cortex Eucommiae, Radix Puerariae, Radix Rehmanniae, Radix Salviae Miltiorrhizae, each 50 gram of the dried medicine of Thallus Laminariae (Thallus Eckloniae), be ground into 50 order fine powders, add Tris buffer (50mmol/L Tris-HCl, the 1mmol/L MgCl of 5 times of volumes
2, 1mmol/L CaCl
2, pH8.0), homogenate, 4 ℃ of lower immersions 12 hours.4 ℃, the centrifugal 15min of 3000rpm gets supernatant.
The method of oozing coagulation with height is filtered the gained supernatant, and filtrate is got in macromolecular substances and the microorganisms such as filtering protein, starch, pectin, tannin, with 4 ℃ of dialysis of 0.5mol/L PBS of the pH7.4 of 50 times of volumes 24 hours, concentrated, drying obtains the G6 agglutinin.Gained protein is carried out quantitative analysis, and the result is that 300g Chinese patent medicine powder obtains G6 agglutinant protein 250mg.
The preparation of embodiment 2:G6 agglutinin
Take by weighing Radix Notoginseng, the Cortex Eucommiae, Radix Puerariae, Radix Rehmanniae, Radix Salviae Miltiorrhizae, each 100 gram of the dried medicine of Thallus Laminariae (Thallus Eckloniae), be ground into 100 order fine powders, add Tris buffer (50mmol/L Tris-HCl, the 1mmol/LMgCl of 5 times of volumes
2, 1mmol/L CaCl
2, pH8.0), homogenate, 4 ℃ of lower immersions 12 hours.4 ℃, the centrifugal 15min of 3000rpm gets supernatant.
The method of oozing coagulation with height is filtered the gained supernatant, and filtrate is got in macromolecular substances and the microorganisms such as filtering protein, starch, pectin, tannin, with 4 ℃ of dialysis of 0.5mol/L PBS of the pH7.4 of 50 times of volumes 24 hours, concentrated, drying obtains the G6 agglutinin.Gained protein is carried out quantitative analysis, and the result is that 600g Chinese patent medicine powder obtains G6 agglutinant protein 550mg.
The preparation of embodiment 3:G6 agglutinin
Take by weighing Radix Notoginseng, the Cortex Eucommiae, Radix Puerariae, Radix Rehmanniae, Radix Salviae Miltiorrhizae, each 70 gram of the dried medicine of Thallus Laminariae (Thallus Eckloniae), be ground into 80 order fine powders, add Tris buffer (50mmol/L Tris-HCl, the 1mmol/LMgCl of 5 times of volumes
2, 1mmol/L CaCl
2, pH8.0), homogenate, 4 ℃ of lower immersions 12 hours.4 ℃, the centrifugal 15min of 3000rpm gets supernatant.
The method of oozing coagulation with height is filtered the gained supernatant, and filtrate is got in macromolecular substances and the microorganisms such as filtering protein, starch, pectin, tannin, with 4 ℃ of dialysis of 0.5mol/L PBS of the pH7.4 of 50 times of volumes 24 hours, concentrated, drying obtains the G6 agglutinin.Gained protein is carried out quantitative analysis, and the result is that 420g Chinese patent medicine powder obtains G6 agglutinant protein 370mg.
Embodiment 4:G6 agglutinin vitality test
The present invention adopts rabbit erythrocyte coagulation experiment to detect the coagulation vigor of agglutinin.
Positive control: Sigma soybean agglutinin standard substance (solution A)
The G6 agglutinin (solution B) of sample: embodiment 1 preparation, the G6 agglutinin (solution C) of embodiment 2 preparations, the G6 agglutinin (solution D) of embodiment 3 preparations
Negative control: 0.5mol/L PBS (solution E)
Extract family's rabbit ear source venous blood, gather erythrocyte, be made into 2% red cell suspension with normal saline.On V-type blood coagulation plate, positive control and sample are pressed respectively the different proportion dilution, every hole adds 25ul, adding 2% fresh red blood cell suspension 25ul, after concussion shakes up, placed perusal 2 hours for 25 ℃, erythrocyte is deposited on V shaped hole bottom and is bright red point-like during without agglutination phenomenon, is netted when agglutination phenomenon is arranged and does not sink.The coagulation situation the results are shown in Table shown in 1 when collecting different extension rate.The result shows, the G6 agglutinin vigor that embodiment 1-3 is prepared and positive control be without significant difference (P＞0.05), the vigor that the G6 agglutinin that utilizes the method for the invention to obtain has the red cell agglutination of making is described, and negative control group is without coagulation.It should be noted that especially the G6 agglutinin for preparing in the embodiment of the invention and still have a good coagulation vigor in the situation that extension rate is lower, illustrate that gained G6 agglutinin of the present invention is better than the soybean agglutinin standard substance in effect.
The different sample rabbit erythrocyte of table 1 agglutination titer relatively
Embodiment 5:G6 agglutinin reaches the inhibition experiment of the side effect that depressor is caused to the blood pressure lowering experiment of hypertensive rat model
The present embodiment utilizes spontaneously hypertensive SHR rat to be model, the positive medicine of Levamlodipine, detect the prepared G6 agglutinin of embodiment 1 hypotensive effect and with the inhibitory action of Levamlodipine use in conjunction to the edema side effect of its retention of sodium and water that causes.
Laboratory animal: spontaneously hypertensive SHR rat, male and female half and half, body weight is at 250-300g.
Positive control: Levamlodipine besylate
Blank: 0.9% normal saline
The G6 agglutinin of experimental group: embodiment 1 preparation
Get 50 of healthy spontaneously hypertensive SHR rats, male and female half and half, body weight are equally divided into 5 groups at 250-300g, and packet mode is as shown in table 2.Adopt gastric infusion, adopt the rat electronic sphygmomanometer for blood pressure, the clear-headed systolic pressure when quiet of tail volumetric method indirect determination rat, respectively before medication, one week of medication, two weeks, three weeks, the end is carried out the arteria caudalis systolic pressure and is measured all around, and observes the edema situation.
Table 2SHR rat packet mode
Experimental result is shown in Table 3, and the result shows, each experimental group and positive controls are compared with the blank group all significant hypotensive effect; The antihypertensive effect of the G6 agglutinin use in conjunction of Levamlodipine and embodiment 1 preparation is better than independent use Levamlodipine and G6 agglutinin group.Aspect the edema side effect, blank group and use in conjunction group all occur without the edema phenomenon, and the Levamlodipine group has 20% edema incidence rate.
Table 3 Levamlodipine and Levamlodipine/G6 agglutinin associating
Use administration to the impact of SHR rat blood pressure
Annotate: # is for comparing P＜0.01 with the blank group; * for to compare P＜0.05 with the Levamlodipine group
The G6 agglutinin of preparation uses separately and has good hypotensive effect among conclusion: the embodiment 1; When uniting use with depressor, its antihypertensive effect is better, and there were significant differences with the blank group, and is better than using separately the situation of depressor; When uniting use with depressor, can eliminate the toxic and side effects that depressor brings.
According to the method described above, the result of the G6 agglutinin acquired results of embodiment 2 and 3 preparations and embodiment 1 is close.
The above only is preferred implementation of the present invention; should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the principle of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (6)
1. G6 agglutinin, it is characterized in that, from Chinese medicine Radix Notoginseng, the Cortex Eucommiae, Radix Puerariae, Radix Rehmanniae, Radix Salviae Miltiorrhizae, Thallus Laminariae (Thallus Eckloniae), obtain by extraction, and contain Radix Salviae Miltiorrhizae acid, Radix Salviae Miltiorrhizae flavone, arasaponin, Radix Rehmanniae saponin, rehmanin, laminaran, Radix Puerariae flavone, Cortex Eucommiae terpene component.
2. one kind prepares the as claimed in claim 1 method of G6 agglutinin, it is characterized in that, may further comprise the steps:
Step 1) takes by weighing Radix Notoginseng, the Cortex Eucommiae, Radix Puerariae, Radix Rehmanniae, Radix Salviae Miltiorrhizae, the dried medicine of Thallus Laminariae (Thallus Eckloniae) by equal in quality, be ground into 50～100 order medicament mixed powder;
Step 2) medicament mixed powder obtained above is added the Tris buffer of 5 times of volumes, homogenate, 4 ℃ of lower immersions 12 hours, centrifugal, get supernatant;
Step 3) the gained supernatant is utilized high method of oozing coagulation filter, remove impurity, obtain containing the filtrate of phytohemagglutinin;
Step 4) with above-mentioned filtrate dialysis, concentrated, dry, obtains the G6 agglutinin.
3. method according to claim 2 is characterized in that step 2) described centrifugal rotating speed is 3000rpm, and the time is 15min, and temperature is 4 ℃.
4. method according to claim 2 is characterized in that step 4) dialysis solution that uses of described dialysis is phosphate buffer.
5. the G6 agglutinin is preparing the purposes of preventing and treating in the cardiovascular and cerebrovascular diseases medicament as claimed in claim 1.
6. method according to claim 6 is characterized in that, described cardiovascular and cerebrovascular disease is hypertension.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108452073A (en) * | 2018-05-14 | 2018-08-28 | 凯普泰（北京）医药科技有限公司 | A kind of composite plant agglutinin and its preparation method and application |
| CN115316587A (en) * | 2021-03-30 | 2022-11-11 | 云南师范大学 | Method for treating agglutinin in pseudo-ginseng flower |
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2011
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108452073A (en) * | 2018-05-14 | 2018-08-28 | 凯普泰（北京）医药科技有限公司 | A kind of composite plant agglutinin and its preparation method and application |
| CN108452073B (en) * | 2018-05-14 | 2020-12-11 | 西安泽富随康生物科技有限公司 | Composite plant lectin and preparation method and application thereof |
| CN115316587A (en) * | 2021-03-30 | 2022-11-11 | 云南师范大学 | Method for treating agglutinin in pseudo-ginseng flower |
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Application publication date: 20130424 |