CN103027190A - Making method of tomato pomace fermented forage - Google Patents

Making method of tomato pomace fermented forage Download PDF

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Publication number
CN103027190A
CN103027190A CN2011101874150A CN201110187415A CN103027190A CN 103027190 A CN103027190 A CN 103027190A CN 2011101874150 A CN2011101874150 A CN 2011101874150A CN 201110187415 A CN201110187415 A CN 201110187415A CN 103027190 A CN103027190 A CN 103027190A
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China
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fermentation
tomato pomace
tomato
fermented forage
forage
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CN2011101874150A
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赵芸君
王力俭
吴坚
桑断疾
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Forage Institute Xinjiang Academy Of Animal Sciences
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Forage Institute Xinjiang Academy Of Animal Sciences
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

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Abstract

The invention belongs to the forage processing field, and relates to a making method of a tomato pomace fermented forage. The making method of the fermented forage comprises the following steps: uniformly stirring fermentation raw materials (a DM base) comprising 65-75% of a tomato pomace, 10-20% of wheat bran, 8-15% of cottonseed meal, 1.5-2.5% of urea and 1.5-2.5% of ammonium sulfate; inoculating Trichoderma koningii, Candida utilis and Saccharomyces cerevisiae according to a ratio of the Saccharomyces cerevisiae to the Candida utilis to the Saccharomyces cerevisiae of 1:1:1-1:2:2, wherein the inoculation amount accounts for 10-15% of a fermentation substrate, and the water content of the fermentation substrate is 45-55% (m/m); and carrying out shallow layer fermentation or submerged fermentation at 28-32DEG C for 2-4d while stirring once each 24h. The tomato pomace fermented forage can be directly used to feed ruminants, or is baked at 45-55DEG C to the water content of about 10% in order to obtain a tomato pomace fermented forage product. The fermentation method has the advantages of small energy consumption, short period and low cost, and the produced tomato pomace fermented forage has a crude protein content of 26% or more (the DM base) and a urea residual amount of 0.1% or less. The fermented forage has a good palatability and can be used for feeding the ruminants.

Description

A kind of preparation method of tomato pomace fermented feed
Technical field:
The present invention relates to a kind of preparation method of tomato pomace fermented feed, belong to field of feed processing.
Background technology:
Xinjiang is the large province of tomato production, and cultivated area reaches more than 770,000 mu.It is estimated that full boundary has more than 30 industrial tomato sauce processing enterprise, 950,000 tons of yearly productive capacities account for more than 90% of the whole nation, and industrial tomato slag output also accounts for more than 90% of the whole nation.Because the tomato processing has very strong seasonality, the production cycle is relatively concentrated, can discharge a large amount of process residues in a short time.Because tomato pomace contains higher moisture (about about 75%) usually, easily corrupt rotten simultaneously.Especially processing the busy season, slag crust after microorganism spontaneous fermentation, acid smelly unpleasant, severe contamination environment, superior resources can not rationally be utilized, cause the wasting of resources.Tomato pomace has certain nutritive value, belongs to middle energy low-protein roughage.
Feed is the material base of aquaculture development, is related to national food safety, food security and aquaculture sustainable development overall situation.Along with I distinguish the fast development of aquaculture, the feed resource shortage has constituted a serious threat to food security and has become the bottleneck that restricts my pastoral industry sustainable development.Because tomato pomace is after artificially adding fermentation auxiliary material wheat bran and inorganic nitrogen-sourced urea, ammonium sulfate, after the useful excellent species fermentations such as saccharomycete, can improve the amount of crude protein level, vitamin, amino acid, somatomedin and the multiple digestive ferment of fermentation substrate, reduce coarse-fibred content, can have good palatability and feeding value, can be applicable to ruminant and produce.In view of this, abundant tomato pomace is primary raw material to this patent take Xinjiang, and replenishes certain carbon nitrogen source, adopts solid microbe fermentation technology, has set up the preparation method of tomato pomace fermented feed.
Summary of the invention:
The object of the present invention is to provide a kind of preparation method of tomato pomace fermented feed.The present invention realizes by following technical proposal:
The used bacterial classification of tomato pomace fermented feed is mainly yeast and mold.Strain combination is candida utili+brewer's yeast+koning trichoderma.Tomato pomace fermented feed fermentation raw material adopts tomato pomace 65~75%, wheat bran 10~20%, cotton dregs 8~15%, urea 1.5~2.5%, ammonium sulfate 1.5~2.5% carries out proportioning, and fermentation raw material can adopt artificial mixing and stirring or use the flat mixer mixing and stirring.Fermentation raw material pH value is controlled at 5.6~6.0 usually, this pH value can suppress the part varied bacteria growing and on yeast without impact.Koning trichoderma is at 28~32 ℃ of lower 4~6d that cultivate, make suspension after, its spore count of microscopy reaches 107~109/ml, saccharomycete is at 28~32 ℃ of lower 1~2d that cultivate, microscopy yeast cell suspension, its cell number is 108~1010/ml.The inoculation strain combination is koning trichoderma: candida utili: brewer's yeast, inoculative proportion join in the fermentation raw material after mixing according to 1: 1: 1~1: 2: 2 ratio.The inoculation total amount is 10~15% of fermentation substrate, its increase and decrease will be grasped by factors such as the freshness of raw material, temperature, purpose is to make the production bacterium form as early as possible advantage, and producing bacterial classification (three-class strain) has a small amount of caking, should pulverize just inoculation under the condition of sealing.Koning trichoderma and candida utili, brewer's yeast stir evenly first according to 1: 1: 1~1: 2: 2 ratio, then bacterial classification inoculation are fully mixed thoroughly behind fermentation substrate, and bacterial classification is evenly attached on the compost.Finally make its fermentation substrate water content reach 45~55% (m/m), if the water content of fermentation substrate does not reach requirement, spray into water, mix while spraying.Also can be adjusted by the relative humidity in the fermenting cellar.Fermentation can be adopted shallow-layer fermentation and submerged fermentation.Shallow-layer fermentation is stacked thickness generally between 3~5cm for material, had better not surpass 3cm when temperature is high, otherwise be difficult to dispel the heat.Room temperature remains between 25~30 ℃.The space humidity regulation is also very important in process of production, and humidity should remain on 80%~90% when fermenting house began to cultivate, and makes the early stopping that fermented, later stage will reduce humidity in order to avoid the evaporation of fermentation material moisture is too fast, makes bacterial strain to the compost growth inside.During final discharging, expect dry and comfortable, about 40% fashion of water content.If can be down to approximately 35%, can save a large amount of heat energy when then drying, reduce cost.Submerged fermentation is large-scale production, generally uses the ventilation koji bed, and material thickness is between 20~30cm, and summer is thinner, and then put thicker winter.Ventilation will be controlled at suitable scope, and temperature is controlled at about 30 ± 2 ℃, crosses to hang down then should be incubated the machine that will blow in when too high blowing cooling fermentation.Every 24h stirs once, takes out afterwards in 2~4 days, and this fermented product needn't be dried and can directly be used for feeding as early as possible ruminant, or places and dry discharging when being 10% left and right sides to moisture under 45~55 ℃, is ground into Powderedly, can get tomato pomace fermented feed product.
The specific embodiment:
The below does an explanation to the present invention.
Fermentation raw material is by tomato pomace 71.5%, wheat bran 15%, and cotton dregs 10%, urea 1.5%, ammonium sulfate 2% forms, and stirs.Strain combination is koning trichoderma: candida utili: brewer's yeast, and inoculative proportion was undertaken by 1: 2: 1, and inoculum concentration is 12% of fermentation substrate, the fermentation substrate water content is 45~55% (m/m), adopts the shallow-layer fermentation, and fermentation temperature is 30 ℃, every 24h stirs once, and 2~4d can finish.Tomato pomace fermented feed crude protein content 〉=26% (DM basis), urea residual quantity≤0.03 (DM basis).
Select lactation age in days (86 ± 19) d, 20 of the Xinjiang brown oxen that 2~3 tires, health, the lactation amount of body weight (467 ± 28) kg is close are divided into 2 groups, check difference not remarkable (P>0.05) through t.Substitute control group daily ration 6% cotton dregs, 4.5% wheat bran and 3.5% corn silage (DM) with 14% tomato pomace fermented feed.The result shows: compare with control group, the Xinjiang brown ox is added hello tomato pomace fermented feed and significantly improves DM feed intake, 4% correction milk production (P<0.05); The milk production of tomato pomace fermented feed group, feed conversion rate, butterfat percnetage, lactoprotein, lactose, total solid solid content numerically are higher than control group (P>0.05).Compare with control group, adopt the tomato pomace fermented feed can significantly improve non-oxidizability index T-SOD in the Xinjiang brown cow's serum in the daily ration, the activity of GSH-px improves the amount of TAOC and the amount (P<0.05) that reduces MDA; Adopt the tomato pomace fermented feed can significantly reduce the content (P<0.05) of MDA in the whey in the daily ration, be improved antioxygenic property index T-SOD in the whey, the trend of the activity of GSH-px and TAOC content (P<0.10).Compare with control group, adopt the tomato pomace fermented feed can significantly improve IgG in the serum, IgM antibody horizontal (P≤0.05) in the daily ration, be improved the trend (P<0.10) of IgA antibody content in the serum, but to IgG, IgA in the whey and IgM antibody horizontal do not make significant difference (P>0.05).Add and feed 1.79 yuan of tomato pomace fermented feed contrast group every increasing benefits every day, amplification 9.68%; Every ox per month (by 30d) can be taken in 53.7 yuan more.Above result shows: the tomato pomace fermented feed can improve production performance, Milk Quality, the general level of the health, antioxygenic property, the immunity of Xinjiang brown ox, antioxygenic property and the economic benefit of milk, is a kind of preferably fermented feed.

Claims (6)

1. the tomato pomace fermented feed is take tomato pomace as the main fermentation composition, and wheat bran, cotton dregs add inorganic nitrogen-sourced urea, ammonium sulfate simultaneously for the fermentation auxiliary material, the tomato pomace fermented product that the saccharomycete of the certain proportioning of warp and mould strain combination ferment under given conditions and obtain.
2. tomato pomace according to claim 1 is the effluent in the tomato process, should include tomato skin, Tomato Seed and a small amount of tomato pulp.
3. tomato pomace fermentation raw material according to claim 1 (DM basis) is by tomato pomace 65~75%, wheat bran 10~20%, and cotton dregs 8~15%, urea 1.5~2.5%, ammonium sulfate 1.5~2.5% forms, and it is rear as fermentation raw material to stir.
4. tomato pomace fermented bacterium according to claim 1 is combined as koning trichoderma: candida utili: brewer's yeast, and inoculative proportion was undertaken by 1: 1: 1~1: 2: 2, and inoculum concentration is 10~15% of fermentation substrate.
5. tomato pomace fermentation substrate water content according to claim 1 is 45~58% (m/m), and fermentation temperature is 28~32 ℃, and every 24h stirs once, and 2~4d can finish.But the fermentation finish after the Direct-fed ruminant, or place dry by the fire under 45~55 ℃ to moisture be about 10%, can get tomato pomace fermented feed product.
6. according to claim 1, the bacterial classification in the 4 described tomato pomace fermented feed preparation methods, it is characterized by koning trichoderma enlarged through agar slant culture-medium, test tube, eggplant bottle respectively with the PDA culture medium and cultivate; Saccharomycete enlarges through agar slant culture-medium, test tube, triangular flask respectively with malt extract medium to be cultivated; Wherein koning trichoderma is at 28~32 ℃ of lower 4~6d that cultivate, make suspension after, its spore count of microscopy reaches 10 7~10 9Individual/ml, saccharomycete is cultivated 1~2d under 28~32 ℃, microscopy yeast cell suspension, and its cell number is 10 8~10 10Individual/ml.
CN2011101874150A 2011-07-06 2011-07-06 Making method of tomato pomace fermented forage Pending CN103027190A (en)

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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104905007A (en) * 2015-06-12 2015-09-16 甘肃农垦天牧乳业有限公司 Preparation method of pumpkin pulp-contained full-value micro-ecology feed for ruminants
CN104957414A (en) * 2015-06-12 2015-10-07 甘肃农垦天牧乳业有限公司 Preparation method of complete micro-ecological feed containing farmland by-products for ruminant animals
CN104970225A (en) * 2015-06-12 2015-10-14 甘肃农垦天牧乳业有限公司 Preparation method of complete micro-ecological feed containing corn cobs for ruminant animals
CN104982711A (en) * 2015-06-12 2015-10-21 甘肃农垦天牧乳业有限公司 Preparation method for complete micro-ecological feed containing sunflower heads for ruminant
CN107095008A (en) * 2017-04-05 2017-08-29 新疆农业职业技术学院 A kind of Quality Protein Maize core feed and preparation method thereof
CN108056260A (en) * 2018-01-11 2018-05-22 宁夏大北农科技实业有限公司 Feed for beef cattle and preparation method thereof and the application in the special cultivation of Muslim beef
IT201700082285A1 (en) * 2017-07-19 2019-01-19 Linfa Scarl Use of vegetable waste for the preparation of bioformulas based on microorganisms beneficial for agriculture
CN114158648A (en) * 2021-11-09 2022-03-11 新疆冠农果茸股份有限公司 Tomato peel residue biological fermentation feed, preparation method and application thereof

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104905007A (en) * 2015-06-12 2015-09-16 甘肃农垦天牧乳业有限公司 Preparation method of pumpkin pulp-contained full-value micro-ecology feed for ruminants
CN104957414A (en) * 2015-06-12 2015-10-07 甘肃农垦天牧乳业有限公司 Preparation method of complete micro-ecological feed containing farmland by-products for ruminant animals
CN104970225A (en) * 2015-06-12 2015-10-14 甘肃农垦天牧乳业有限公司 Preparation method of complete micro-ecological feed containing corn cobs for ruminant animals
CN104982711A (en) * 2015-06-12 2015-10-21 甘肃农垦天牧乳业有限公司 Preparation method for complete micro-ecological feed containing sunflower heads for ruminant
CN107095008A (en) * 2017-04-05 2017-08-29 新疆农业职业技术学院 A kind of Quality Protein Maize core feed and preparation method thereof
IT201700082285A1 (en) * 2017-07-19 2019-01-19 Linfa Scarl Use of vegetable waste for the preparation of bioformulas based on microorganisms beneficial for agriculture
CN108056260A (en) * 2018-01-11 2018-05-22 宁夏大北农科技实业有限公司 Feed for beef cattle and preparation method thereof and the application in the special cultivation of Muslim beef
CN108056260B (en) * 2018-01-11 2021-11-16 宁夏大北农科技实业有限公司 Feed for beef cattle and preparation method thereof
CN114158648A (en) * 2021-11-09 2022-03-11 新疆冠农果茸股份有限公司 Tomato peel residue biological fermentation feed, preparation method and application thereof

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Application publication date: 20130410