CN103006904A - Healthcare product with function of strengthening immunity - Google Patents
Healthcare product with function of strengthening immunity Download PDFInfo
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- CN103006904A CN103006904A CN2012105934475A CN201210593447A CN103006904A CN 103006904 A CN103006904 A CN 103006904A CN 2012105934475 A CN2012105934475 A CN 2012105934475A CN 201210593447 A CN201210593447 A CN 201210593447A CN 103006904 A CN103006904 A CN 103006904A
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Abstract
The invention relates to a healthcare product with a function of strengthening the immunity, and specifically relates to a preparation process and an application of the healthcare product. The prescription combination is carried out under the instruction of the combination rule of the traditional Chinese medicine according to the theory of the traditional Chinese medicine, and the specific prescription is as follows: 5 parts of pilose antler, 10 parts of American ginseng, 30 parts of radix astragali and 20 parts of medlar; the healthcare product is extracted by using a reflux method; the best extraction condition is chosen according to an orthogonal experiment theory, and finally the oral liquid is prepared; and proved by an animal experiment, the healthcare product has the function of strengthening the immunity.
Description
Technical field
The present invention relates to a kind of health product with enhancing immunity effect, be specifically related to preparation technology of this health product and uses thereof.
Background technology
Immunity is the defense mechanism of human body self, be human body identification and any foreign body (virus, antibacterial etc.) of eliminating external intrusion, process the ability of self cell and identification and processing vivo mutations cell and the virus infected cell of old and feeble, damage, death, degeneration.The health of hypoimmunity is easy to infected or cancer stricken; and a lot of reasons can make immune system normally not bring into play protective effect, in the case; very easily cause the infection such as antibacterial, virus, fungus, thus hypoimmunity the most directly to show be exactly liable to illness.
According to the literature, Cornu Cervi Pantotrichum, Radix Panacis Quinquefolii, the Radix Astragali, Fructus Lycii all contain the composition of enhancing immunity, and in the middle of various compositions, polysaccharide then plays an important role.For example, the Chen Junhui effect of once mentioning the enhancing immunity of Radix Panacis Quinquefolii polysaccharide is better than Radix Panacis Quinquefolii saponin.The polysaccharide component of other three flavors medicines also has the relevant report of enhancing immunity.According to theory of Chinese medical science, the drug matching rule, the present invention carries out compatibility with these four medical materials.The research of compound preparation of the present invention has certain meaning to the immunity aspect that strengthens the people.
Summary of the invention
Purpose of the present invention provides a kind of new health product with enhancing immunity effect, and these health product can strengthen the immunologic function of human body, prevent disease is had good effect, and have no side effect.
The property of medicine of single medicinal material of the present invention:
1, Cornu Cervi Pantotrichum:
Sweet in the mouth, warm in nature.Function cures mainly: the main stagnant blood that leaks down, cold and heat infantile convulsion, the strong will of QI invigorating, the population are not old.
2, Radix Panacis Quinquefolii:
Sweet in the mouth, little hardship.Function cures mainly: boosting qi and nourishing yin, relieve inflammation or internal heat and promote the production of body fluid.
3, the Radix Astragali:
Sweet in the mouth, warm in nature.Function cures mainly: tonifying Qi and lifting yang, consolidating superficial resistance benefit are defended, expelling pus and toxin by strengthening QI, inducing diuresis to remove edema.
4, Fructus Lycii:
Nature and flavor are sweet flat.Function cures mainly: invigorating the liver and kidney, beneficial vital essence, longue meat, improve complexion, make eye bright and calm the nerves, dispel the wind and control void, life lengthening, hard muscles and bones.
This four flavors Chinese crude drug is carried out compatibility according to theory of Chinese medical science, have the performance direction, the expansion therapeutic domain that strengthen the efficacy of a drug, generation synergism, the multi-functional single Chinese medicine of control, adapt to the characteristics such as toxic and side effects of the state of an illness, control medicine.In the middle of this four Chinese medicine, Cornu Cervi Pantotrichum, two Radix Panacis Quinquefolii are principal agent, and the Radix Astragali, Fructus Lycii are accessory drugs, and the usage and dosage of prescription meets drug matching principle and rule.
This health product have the effect of enhancing immunity, the liver and the kidney tonifying, nourishing YIN and benefiting blood, heat clearing away spleen invigorating, damp eliminating detoxifcation, and the patient who treats hypoimmunity is had remarkable result; Herbal medicine efficacy of the present invention is reliable, and side effect is little, and indication is wide, can improve the immunity of body, and the disease that hypoimmunity is caused has prevention and therapeutic effect.
Make Chinese medicine preparation (consumption is weight portion) according to following prescription:
Cornu Cervi Pantotrichum 5-10 part Radix Panacis Quinquefolii 10-15 part Radix Astragali 20-30 part Fructus Lycii 15-20 part
Concrete preparation method of the present invention is:
Circumfluence method is adopted in this experiment, adopts orthogonal experiment method that the extraction process of polysaccharide is carried out preferably, and single factor is set to consumption, extraction time and the extraction time of water.Extracting solution carries out content detection to polysaccharide, selects as the case may be best extraction conditions to extract in a large number, then makes oral liquid, after testing qualified laggard action thing experiment.
The specific embodiment
One preparation technology
The inventor passes through long term test and courageously gropes, and sums up various components and the proportioning thereof that draws medicine of the present invention on the basis of great number tested data, and proportioning is 5 parts in Cornu Cervi Pantotrichum, 10 parts of Radix Panacis Quinquefoliis, 30 parts of the Radixs Astragali, 20 parts of Fructus Lyciis.Adopt circumfluence method to extract, make at last oral liquid.
Example 1
(1) carries out dedusting after crude drug Cornu Cervi Pantotrichum, Radix Panacis Quinquefolii, the Radix Astragali and the Fructus Lycii buying, then clean, under 30 ℃-55 ℃, carry out drying, be used for extracting after quality examination is qualified;
(2) get respectively 5 parts in Cornu Cervi Pantotrichum, 10 parts of Radix Panacis Quinquefoliis, 20 parts of the Radixs Astragali, 15 parts of Fructus Lyciis, adopted 8-12 volume cold water soak 1 hour; Carry out reflux, extract, under 90 ℃ condition, extraction time is 1-3 time, and extraction time is 1-3h; Extracting solution is crossed 200 mesh sieves, removes impurity, places 4 ℃ of refrigerators to leave standstill 24h; Take out from refrigerator, ultrasonic 8min is centrifugal 10min under the condition of 7000r/min at rotating speed, removes precipitation;
(3) making of glucose standard curve: get the storing solution that dextrose standard sample after the constant weight is configured to 1mg/mL, then get respectively storing solution 5mL, 6mL, 7mL, 8mL, 9mL is settled to 100mL, the glucose solution 0.5mL that gets variable concentrations places test tube, the phenol (5%) that at first adds 1mL, then the concentrated sulphuric acid that adds 3.5mL, shake up water-bath 30min in the thermostat water bath that is placed on 40 ℃, after the water-bath, room temperature detects in ultraviolet spectrophotometer 490nm place after placing 15min, draws at last the standard curve of glucose;
(4) measurement of the polysaccharide content of each sample extracting solution after process step (2) is processed; Adopt the phenolsulfuric acid method to measure the content of polysaccharide in the medicinal liquid, sample thief 0.5mL places test tube respectively, the concentrated sulphuric acid that at first adds 3.5mL, then the phenol (5%) that adds 1mL, shake up water-bath 30min in the thermostat water bath that is placed on 40 ℃, after the water-bath, room temperature detects in ultraviolet spectrophotometer 490nm place after placing 15min, calculates the content of polysaccharide according to standard curve;
(5) select optimised process after, the volume of original liquid is concentrated into original 1/6, in 4 ℃ of refrigerators, place stand-by.
Example 2
(1) carries out dedusting after crude drug Cornu Cervi Pantotrichum, Radix Panacis Quinquefolii, the Radix Astragali and the Fructus Lycii buying, then clean, under 30 ℃-55 ℃, carry out drying, be used for extracting after quality examination is qualified;
(2) get respectively 5 parts in Cornu Cervi Pantotrichum, 12 parts of Radix Panacis Quinquefoliis, 24 parts of the Radixs Astragali, 20 parts of Fructus Lyciis, adopted 8-12 volume cold water soak 1 hour; Carry out reflux, extract, under 90 ℃ condition, extraction time is 1-3 time, and extraction time is 1-3h; Extracting solution is crossed 200 mesh sieves, removes impurity, places 4 ℃ of refrigerators to leave standstill 24h; Take out from refrigerator, ultrasonic 8min is centrifugal 10min under the condition of 7000r/min at rotating speed, removes precipitation;
(3) making of glucose standard curve: get the storing solution that dextrose standard sample after the constant weight is configured to 1mg/mL, then get respectively storing solution 5mL, 6mL, 7mL, 8mL, 9mL is settled to 100mL, the glucose solution 0.5mL that gets variable concentrations places test tube, the phenol (5%) that at first adds 1mL, then the concentrated sulphuric acid that adds 3.5mL, shake up water-bath 30min in the thermostat water bath that is placed on 40 ℃, after the water-bath, room temperature detects in ultraviolet spectrophotometer 490nm place after placing 15min, draws at last the standard curve of glucose;
(4) measurement of the polysaccharide content of each sample extracting solution after process step (2) is processed; Adopt the phenolsulfuric acid method to measure the content of polysaccharide in the medicinal liquid, sample thief 0.5mL places test tube respectively, the concentrated sulphuric acid that at first adds 3.5mL, then the phenol (5%) that adds 1mL, shake up water-bath 30min in the thermostat water bath that is placed on 40 ℃, after the water-bath, room temperature detects in ultraviolet spectrophotometer 490nm place after placing 15min, calculates the content of polysaccharide according to standard curve;
(5) behind the optimum technology, the volume of original liquid is concentrated into original 1/4, in 4 ℃ of refrigerators, places stand-by.
Example 3
(1) carries out dedusting after crude drug Cornu Cervi Pantotrichum, Radix Panacis Quinquefolii, the Radix Astragali and the Fructus Lycii buying, then clean, under 30 ℃-55 ℃, carry out drying, be used for extracting after quality examination is qualified;
(2) get respectively 8 parts in Cornu Cervi Pantotrichum, 12 parts of Radix Panacis Quinquefoliis, 20 parts of the Radixs Astragali, 16 parts of Fructus Lyciis, adopted 8-12 volume cold water soak 1 hour; Carry out reflux, extract, under 90 ℃ condition, extraction time is 1-3 time, and extraction time is 1-3h; Extracting solution is crossed 200 mesh sieves, removes impurity, places 4 ℃ of refrigerators to leave standstill 24h; Take out from refrigerator, ultrasonic 8min is centrifugal 10min under the condition of 7000r/min at rotating speed, removes precipitation;
(3) making of glucose standard curve: get the storing solution that dextrose standard sample after the constant weight is configured to 1mg/mL, then get respectively storing solution 5mL, 6mL, 7mL, 8mL, 9mL is settled to 100mL, the glucose solution 0.5mL that gets variable concentrations places test tube, the phenol (5%) that at first adds 1mL, then the concentrated sulphuric acid that adds 3.5mL, shake up water-bath 30min in the thermostat water bath that is placed on 40 ℃, after the water-bath, room temperature detects in ultraviolet spectrophotometer 490nm place after placing 15min, draws at last the standard curve of glucose;
(4) measurement of the polysaccharide content of each sample extracting solution after process step (2) is processed; Adopt the phenolsulfuric acid method to measure the content of polysaccharide in the medicinal liquid, sample thief 0.5mL places test tube respectively, the concentrated sulphuric acid that at first adds 3.5mL, then the phenol (5%) that adds 1mL, shake up water-bath 30min in the thermostat water bath that is placed on 40 ℃, after the water-bath, room temperature detects in ultraviolet spectrophotometer 490nm place after placing 15min, calculates the content of polysaccharide according to standard curve;
(5) behind the optimum technology, the volume of original liquid is concentrated into original 1/5, in 4 ℃ of refrigerators, places stand-by.
3 above-mentioned resulting medicinal liquids of example are made oral liquid, and specific implementation process is as follows:
(1) adjuvant of selecting is: sucrose, potassium sorbate, glycerol, folic acid, sodium citrate, vitamin B
1, vitamin B
2, vitamin B
6
(2) take out respectively medicinal liquid among the example 1-3, add respectively glycerol 2mL, sucrose 4g, potassium sorbate 0.2g, folic acid 0.4g, vitamin B
10.2g, vitamin B
20.2g, vitamin B
60.2g transferring pH value with sodium citrate at last is 3.8;
(3) fully behind the mixing, the 30min-45min that sterilizes under 120 ℃, the condition of 105KPa places 4 ℃ of refrigerators to leave standstill 24h.
(4) oral liquid after will leaving standstill respectively carries out measurement of the polysaccharide content.Make at last the oral liquid that specification is the 50mL/ bottle, detect qualified rear packing.
Through above each experimentation, again in conjunction with practical situation, the final prescription of selecting of the present invention is 5 parts in Cornu Cervi Pantotrichum, 10 parts of Radix Panacis Quinquefoliis, 30 parts of the Radixs Astragali, 20 parts of Fructus Lyciis, makes the laggard action thing experiment of oral liquid.
Two zooperies
Cornu Cervi Pantotrichum, Radix Panacis Quinquefolii, the Radix Astragali, wolfberry fruit extract are to the effect of the enhancing immunity of hypoimmunity mice:
1, laboratory animal
Use inbred mouse, 18-22g, single sex, every group of 10-15 is only.
2, dosage grouping and given the test agent give the time
Three dosage groups, a negative control group and a positive controls are established in each experiment, and positive control is chosen as commercially available peak spring Korean Ginseng essence.Take 10 times of human body recommended amounts as one of them dosage group, other establishes two dosage groups, and it is 30-45d that given the test agent gives the time.
3, the foundation of immunosuppressant model
Model group: all mices are carried out the foundation of hypoimmunity model, carry out random packet after the modeling, be respectively blank group, low dose group, middle dosage group, high dose group and positive controls, selected modeling reagent is cyclophosphamide.
4, detect index and assay method
(1) internal organs ratio
Weigh rear mortar of mice is put to death, and takes out spleen and thymus, removes most fascia, inhales thousand organ surface blood stains with filter paper.Then weigh, calculate spleen, thymus and body weight ratio, its determination data sees Table 1, table 2.
The impact of table 1 Oral Liquid On Mice body weight of the present invention
The P value: each experimental group contrasts from group with empty
By as seen from Table 1, the initial body weight of mice there was no significant difference (p＞0.05) relatively between middle and high dosage group and blank group, namely the initial body weight of mice is comparatively balanced between each group.After oral administration gave oral liquid 30h of the present invention, the weightening finish of each dosage group mice relatively had significant difference (p＜0.05) between low, positive controls and blank group, i.e. the present invention is influential to the Mouse Weight weightening finish.
The impact of table 2 Oral Liquid On Mice internal organs of the present invention/body weight ratio
The P value: each experimental group contrasts with blank group, and P1 is spleen/body weight ratio, and P2 is thymus/body weight ratio
By as seen from Table 1, the initial body weight of mice there was no significant difference (p＞0.05) relatively between middle and high dosage group and blank group, namely the initial body weight of mice is comparatively balanced between each group.After oral administration gave oral liquid 30h of the present invention, the weightening finish of each dosage group mice relatively had significant difference (p＜0.05) between low, positive controls and blank group, i.e. the present invention is influential to mice organs/body weight ratio.
(2) mensuration of half hemolysis value (HC50)
Get Sanguis caprae seu ovis, normal saline washing 3 times, every Mus carries out immunity through lumbar injection 2% (V/V prepares with normal saline) hematocrit SRBC 0.2ml.After 5 days, extract eyeball and get blood in centrifuge tube, place approximately 1h, solidification blood and tube wall are peeled off, serum is fully separated out, the centrifugal 10min of 2000r/min, with the SA buffering serum is diluted 400 times, getting 1mL puts in vitro, add successively 10% (V/V prepares with buffer) hematocrit SRBC 0.5mL. complement 1ml (V/V dilutes by 1: 10 with the SA buffer) and establish in addition the not control tube of increase serum (replacing with the SA buffer), after putting in 37 ℃ of waters bath with thermostatic control insulation 30min, the ice bath cessation reaction.The centrifugal 10min of 2000r/min gets supernatant 1mL, adds Dou Shi reagent 3ml.Get simultaneously the hematocrit SRBC 0.25mL of 10% (V/V joins with the SA buffer).Add Dou Shi reagent 4ml in another test tube, after fully mixing is placed 10min, make blank in 540nm place control tube, measure respectively and respectively manage optical density value.Optical density value * the extension rate of the amount of hemolysin during with half hemolysis value (HC50) expression (see Table 3, be calculated as follows) sample HC50=sample optical density value/SRBC HD50
The impact of table 3 Oral Liquid On Mice half hemolysis value of the present invention (HC50)
The P value: each experimental group compares with blank group
(3) the MTT labelling ConA mouse spleen lymphocyte conversion test of inducing
The aseptic spleen of getting places the little plate that fills an amount of aseptic Hank ' s liquid, makes cell suspension.Use Hank ' s liquid to wash 3 times, each centrifugalize 10min (1000r/min).Then with cell suspension in the complete culture solution of 2mL, the living cell counting number, adjusting cell concentration is 2 * 10
6Individual/mL, again cell suspension is divided two holes to add in 24 well culture plates, every hole 1mL, a hole adds 50 μ LConA liquid (being equivalent to 5mg/ml) therein, and 5%CO is put in contrast in another hole
2, cultivate 72h in 37 ℃ of incubators.Cultivate and finish front 4h, every hole sucks supernatant 0.7mL gently, adds 0.7mL and does not contain the RPMl1640 culture fluid that calf blood is asked, and adds simultaneously MTT (5mg/ml) 5Mol/ hole, continues to cultivate 4h.Cultivate and finish rear every hole adding 1mL acid isopropyl alcohol, the piping and druming mixing dissolves purple crystal fully.Then this liquid is moved in people's cuvette colorimetric determination on 722 spectrophotometers, wavelength 570nm.The Proliferation of lymphocytes ability deducts the optical density value that does not add the ConA hole with the optical density value that adds the ConA hole and represents, experimental data sees Table 4.
The impact of the mouse spleen lymphocyte conversion test that table 4 ConA induces
The P value: each experimental group compares with blank group
By as seen from Table 4, oral administration gives the oral liquid 30d of the present invention of mice various dose, its lymphocytic energization power relatively has significant difference (P＜0.05) between high dose group and blank group, compare there was no significant difference (P＞0.05) between low, middle dosage group, positive controls and blank group, namely the oral liquid of the present invention of high dose can improve the mouse lymphocyte conversion capability that ConA induces.
(4) delayed allergy (DTH)
Get Sanguis caprae seu ovis, normal saline washing 3 times, every Mus is through lumbar injection 2% (V/V, prepare with normal saline) hematocrit SRBC:(200r/min, 10min) 0.2mL, after the sensitization 4 days, measurement is at right sufficient sole of the foot foot thickness, and same position is measured 3 times, averages.Then at measuring point subcutaneous injection 20% (V/V, prepare with normal saline) hematocrit SRBC μ L sufficient sole of the foot section thickness about 24h measures after the injection, difference (swelling degree of the paw) with sufficient sole of the foot thickness before and after attacking represents the DTH degree, and its impact sees Table 5.
Table 5 oral liquid of the present invention is on the impact of delayed allergy (DTH) test
The P value: each experimental group compares with blank group
By as seen from Table 5, oral administration gives the oral liquid 30d of the present invention of mice various dose, and its swelling degree of the paw comparison there was no significant difference (p＞0.05) between basic, normal, high dosage group, positive controls and blank group is that the delayed metamorphosis of Oral Liquid On Mice of the present invention should be without impact.
(5) improvement antibody-producting cell detection method
Get Sanguis caprae seu ovis, normal saline washing 3 times, every Mus is through lumbar injection 2% (V/V is with the preparation of Sal water) hematocrit SRBC0.2mL.The mice of SRBC immunity after 5 days put to death, take out spleen, make cell suspension.With the agarose heating for dissolving, mix with the double Hank ' s of equivalent liquid, the packing small test tube, every pipe 0.5mL, in pipe, add 10% (V/V prepares with the SA buffer) hematocrit SRBC 50mL, splenocyte suspension 20mL again, after being mixed rapidly, be poured into brush agarose fall the layer slide on, after agar solidifies, the slide level buckled be placed on the horse, put incubation 1.5h in people's CO2 gas incubator, then the complement (1: 10) with the dilution of SA buffer joins in the slide frame groove, after continuing incubation 5h, counting hemolysis plaque number the results are shown in Table 6.
The impact of table 6 Oral Liquid On Mice antibody-producting cell test of the present invention
The P value: each experimental group compares with blank group
By as seen from Table 6, oral administration gives the oral liquid 30d of the present invention of mice various dose, its antibody-producting cell number compares there was no significant difference (P＞0.05) between basic, normal, high dosage group, positive controls and blank group, namely the antibody-producting cell number of Oral Liquid On Mice of the present invention is without impact.
(6) mice carbon clearance test
The india ink of mouse tail vein injection dilution in 1: 35 treats that prepared Chinese ink injects immediately timing.2min, 10min get blood 20 μ L from the angular vein clump respectively after injecting prepared Chinese ink, and it is added among the 2mL.With 722 spectrophotometers at 600nm wavelength place's photometry density value (OD), with Na
2CO
3Solution is made blank.Mice is put to death, take out liver and spleen and weigh.Be calculated as follows phagocytic index a, the results are shown in Table 7.
K＝(LogOD
1-LogOD
2)/(t
2-t
1)
The cubic root of a=body weight ÷ (liver weight+spleen is heavy) * K
The impact of table 7 Oral Liquid On Mice carbon of the present invention clearance test
The P value: each experimental group compares with blank group
By as seen from Table 7, oral administration gives the oral liquid 30d of the present invention of mice various dose, its phagocytic index there was no significant difference (P＞0.05) relatively between middle and high dosage group and blank group, and relatively have between low dose group, positive controls and blank group significant difference (P＜0.05) be the carbon of Oral Liquid On Mice of the present invention clean up test influential.
In sum, the body weight of Oral Liquid On Mice of the present invention and internal organs/body weight ratio is influential, all influential to three dosage groups of mice half hemolysis value (HC50), the oral liquid of the present invention of high dose can improve the mouse lymphocyte conversion capability that ConA induces, delayed metamorphosis on mice should be without impact, on the antibody-producting cell number of mice without impact, low dose group to the carbon of mice clean up the experiment influential, can draw the oral liquid that the present invention obtains and have the effect that strengthens mouse immunity, provide foundation to the research that improves human immunity.
Claims (4)
1. prescription with health product of enhancing immunity:
1) according to theory of Chinese medical science, under the guidance of the compatibility rule of Chinese materia medica, the combination of filling a prescription is specifically filled a prescription as follows: 5 parts in Cornu Cervi Pantotrichum, 10 parts of Radix Panacis Quinquefoliis, 30 parts of the Radixs Astragali, 20 parts of Fructus Lyciis;
2) functional component in this prescription is polysaccharide component, and according to pertinent literature, contained polysaccharide component all has the effect of enhancing immunity in Cornu Cervi Pantotrichum, Radix Panacis Quinquefolii, the Radix Astragali, the Fructus Lycii.
2. preparation technology:
1) extract these health product and adopt circumfluence method to extract under 90 ℃, according to the theoretical extraction conditions of selecting the best of orthogonal experiment, single factor of orthogonal experiment is chosen to be consumption, extraction time and the extraction time of water;
2) the final preparation type of selecting of these health product of preparation is oral liquid, and the adjuvant of selection is: sucrose, potassium sorbate, glycerol, folic acid, sodium citrate, vitamin B1, vitamin B2, vitamin B6.
3. such as prescription and the preparation technology of claim 1 and claimed in claim 2 health product, have the characteristics such as reasonability is strong, simple, realistic production.
4. according to the made healthcare dietetic liquid of this prescription, by the analysis to animal experimental data, has the effect of enhancing immunity.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107307423A (en) * | 2017-07-12 | 2017-11-03 | 吉林青晨药业有限公司 | Product and preparation method with regulation enhancing body's immunity |
| CN114794477A (en) * | 2022-04-29 | 2022-07-29 | 青海师范大学 | Composition for resisting fatigue and improving immunity and preparation method thereof |
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| CN1672721A (en) * | 2004-03-25 | 2005-09-28 | 香港理工大学 | Immunity raising Chinese medicine and its prepn process |
| CN1935010A (en) * | 2006-09-15 | 2007-03-28 | 瑞丽民族制药有限公司 | Health food for intensifying body immunological competence and improving memory function, and its preparing method |
| CN101822746A (en) * | 2009-03-04 | 2010-09-08 | 北京因科瑞斯医药科技有限公司 | Chinese medicinal composition with function of enhancing immunity and preparation method thereof |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1672721A (en) * | 2004-03-25 | 2005-09-28 | 香港理工大学 | Immunity raising Chinese medicine and its prepn process |
| CN1935010A (en) * | 2006-09-15 | 2007-03-28 | 瑞丽民族制药有限公司 | Health food for intensifying body immunological competence and improving memory function, and its preparing method |
| CN101822746A (en) * | 2009-03-04 | 2010-09-08 | 北京因科瑞斯医药科技有限公司 | Chinese medicinal composition with function of enhancing immunity and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107307423A (en) * | 2017-07-12 | 2017-11-03 | 吉林青晨药业有限公司 | Product and preparation method with regulation enhancing body's immunity |
| CN114794477A (en) * | 2022-04-29 | 2022-07-29 | 青海师范大学 | Composition for resisting fatigue and improving immunity and preparation method thereof |
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Application publication date: 20130403 |