CN103004663A - Pelteobagrus fulvidraco artificial propagation process - Google Patents
Pelteobagrus fulvidraco artificial propagation process Download PDFInfo
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- CN103004663A CN103004663A CN2012105623480A CN201210562348A CN103004663A CN 103004663 A CN103004663 A CN 103004663A CN 2012105623480 A CN2012105623480 A CN 2012105623480A CN 201210562348 A CN201210562348 A CN 201210562348A CN 103004663 A CN103004663 A CN 103004663A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention relates to a pelteobagrus fulvidraco artificial propagation process, which comprises parent culture, injection of induction medicine, production and placement quantity of egg collectors, time for taking the egg collectors and washing the eggs, incubation and temporary culture and time for releasing into pools, wherein requirements for the parent are as follows: the mating ratio of two-year hen which is over 75g and milter which is over 150g is (2-3):1. After the primary induction injection in the time period of 14:00 to 19:00, the parents are released into an egg laying pool, one kilogram of induction medicine contains 3 to 5mg of dissolved organic matter (DOM), 10 to 39 micrograms of lutein releasing hormone (LRH-A2) and 800 to 1200IU of human chorionic gonadotropin (HCG), and the quantity of the egg collectors is placed according to a ratio with the milter of 1:1. The time for washing the eggs falls in between the last phase of egg development gastrulae and the formation phase of a tail tooth, and an incubation way for combining a cylinder with a cement pool is adopted. The process is simple to operate, fish fries with high yield and consistent development can be obtained, an order-oriented production can be realized, and the problem that the pelteobagrus fulvidraco does not naturally lay eggs in high-temperature seasons can be solved.
Description
Technical field
The present invention relates to the fresh-water fishes artificial fecundation method, particularly yellow cartfish artificial propagation technique.
Background technology
Pelteobagrus is in the fish that several times a year lay eggs, and the repoductive time span is large, since May, lasts till by the end of August, and therefore can carry out repeatedly fry in the yellow cartfish breeding production sends out the pool.The propagation method of at present existing yellow cartfish is various; most importantly carry out " gametocide is got essence " formula artificial propagation and pond natural spawning; yet these two kinds of propagation methods exist some shortcomings, and the former production cost is higher, labour intensity is larger, and the production scale degree is not high.And the latter has relatively high expectations to pond quantity configuration, production and sales scale, and climate and water quality impact are remarkable, has affected the supply scale of advanced fry of yellow catfish.At first, " gametocide is got essence " formula artificial propagation mode is because the yellow cartfish individuality is little, and fish brood amount is less, causes total man worker's productive labor intensity too large; Along with a road of fry price drops, it is obviously higher to produce relative cost, is difficult to improve the production scale degree.Secondly, 1. pond natural spawning mode shortcoming is because natural spawning soil pond parent's breeding density only has 2250kg-3000kg/hm
2, density increases can affect parent's normal spawning, and these parents are not because carry out artificial induced spawning, and egg-laying time disperses very much, criticizes the quantity of emerging for the list that guarantees every day like this, needs dispose a large amount of pond and parent; 2. natural spawning is subjected to the interference of the extraneous factors such as water quality, temperature very large, and water quality is bad or change and do not lay eggs when large, does not lay eggs when climatic environment changes greatly, will have a strong impact on the plan of emerging.3. the uncontrollable initiative of emerging of natural spawning.Early, the later stage output of seedling is few, price is high, but can't increase output of seedling; Forge during emergence peak in mid-term, market is large for the seedling amount, price is low, sales difficulty, emerges but can't suppress it.
At last, because natural spawning mode water mold generation in hatching process in pond is serious, for preventing the diffusion of water mold, improve incubation rate and emergence rate, can use some medicines to prevent the water mold diffusion, therefore must have influence on the quality of seed, for hidden danger has been buried in the from now on growth of fry.Yet when summer high temperature fry to send out the survival rate on the pool higher, growth rate is very fast, " gametocide is got essence " formula artificial propagation mode and pond natural spawning mode all are difficult to satisfy the needs of large-scale production.
Summary of the invention
Shortcoming for existing yellow cartfish propagation technique; not enough for solving when the summer high temperature fry market supply; overcome the single batch of present situation that can not form fairly large output, the invention provides a kind of yellow cartfish biological control new technology, can carry out the production of order formula scale advanced fry of yellow catfish.
The present invention be intended to solve the prior art problem one of at least.One aspect of the present invention provides a kind of yellow cartfish artificial propagation technique, may further comprise the steps:
A, parent culture: select the yellow cartfish parent by male and female proportioning 2～3: 1 combo;
B, oxytocic drug injection: carry out 1 oxytocic drug injection;
C, fish nest and placement quantity: put into spawning pond after the injection, the fish nest is identical with male parent mantissa in the spawning pond, and parent's mating is originated in fertilized egg in the fish nest;
D, get the fish nest and wash ovum: lay eggs and the fish nest is taken out after finishing, the palm sheet in the fish nest is carried out rinsing in clear water, collect the fish-egg that washes;
E, hatching and foster temporarily: the fish-egg that will wash collection is put in throws hatching in the cylinder, be put in behind the fry membrane in the cement pit and support temporarily, palm sheet after simultaneously fish-egg being cleaned hangs on the cement pit yoke hatches, and behind the fry membrane palm sheet and yoke is taken out, and supports temporarily in cement pit;
F, a pool time: treat the complete taking-up of fry membrane palm sheet in the hatchery, clean, continue to be put in to support temporarily in the cement pit and treat a pool.
According to concrete example of the present invention, in parent culture, the parent is with 6000～7500kg/hm
2Put in a suitable place to breed, the front water temperature of preferably surviving the winter is thrown something and fed 〉=40% protein feed with 1～1.5% amount more than 12 ℃ the time, the depth of water 1.8～2.0m, transparency is not less than 30em, be adjusted into 1.0～1.2m with the depth of water spring, water temperature begins the feed of throwing something and feeding after being higher than 12 ℃, admixes vitamin E, Vc and immunity additive in feed, with the throw something and feed feed of albumen≤38% of 1% amount.Draw in the net to pick out during preferred breeding maturity preferably the parent be used for hastening parturition.
According to concrete example of the present invention, in the oxytocic drug injection, adopt 1 injection of abdominal cavity pectoral fin, be between 14:00～19:00 inject time, use 0.7% physiological saline preparation liquid medicine injection, oxytocic species amount and dosage are every kilogram of PG3～5mg or DOM3～5mg+LRH-A
210～30 μ g+HCG800～1200IU, needle tubing inserts the about 0.5-1cm of pectoral fin base portion, and every tail is injected the 1ml liquid approximately, the same dosage of female milter.
According to concrete example of the present invention, the fish nest is made when placing, and fish nest preparation method is that to select the black plastic drum of high 25cm, internal diameter 30cm be the breeding fish nest, and the palm sheet is placed in the bottom, fastens with the 8# galvanized wire.Wherein the palm sheet through cutting out, rinsing and the operation such as tan by the sun after use.The fish nest lies in a horizontal plane in the cement pit of laying eggs, and quantity is identical with milter parent mantissa, density is 6-8/m
2Place, the cement pit depth of water of laying eggs 0.6～0.8m, female milter proportioning is 2～3: 1.
According to concrete example of the present invention, get the fish nest and wash the ovum time, getting the fish nest time is 40.5～42h after the yellow cartfish injection, the dried up time of palm sheet is no more than 15min, preferably wash the ovum time and spending primitive gut latter stage between the tail dentification phase, will be placed with clear water, increase oxygen gas head, clean in the cement pit of miniflow water with the palm sheet of fish-egg.Can adopt the form of rocking gently back and forth.Fish-egg washed down from the palm sheet can effectively reduce water mold and infect fish-egg, improve incubation rate.
According to concrete example of the present invention, hatching is when supporting temporarily, and hatching is adopted and thrown the cylinder hatching mode that combines with the cement pit hatching, throws the hatching pail of cylinder hatching employing inverted conical inner wall smooth, will wash the fish-egg of collection with 60～800,000/m
3Density is thrown in wherein, and 28～34h is supported in water hatching that the bottom is through-flow temporarily, and is behind the fry demoulding that its rinsing is clean, with 8～100,000 tails/m
2Support temporarily in the mud of pond, the depth of water 0.1～0.3m, dissolved oxygen DO 〉=5mg/L sends out the pool after supporting temporarily 40～64h during 24～29 ℃ of water temperatures; Palm sheet after egg white is washed in cement pit hatching employing is with 23～27/m
2Hang on the cement pit yoke, place and to increase oxygen gas head, miniflow water, the depth of water 0.4～0.5m behind the fry membrane takes out palm sheet and yoke, and sucking-off fry when treating that fry is high-visible is with foul impurity rinsing removal, with 8～100,000 tails/m
2Support temporarily in the mud of pond.The preferred hatching pail that cylinder adopts the stainless steel and iron leather work of inverted conical inner wall smooth, the about 0.5m of volume of throwing
3, high 1.4m, internal diameter 1.2m.
According to concrete example of the present invention, send out the pool time, water temperature is in the time of 23～27 ℃, and the membrane time needs two days, and spray is 240～250 tails/g in the time of the 5th day; Or water temperature when reaching more than 28～31.5 ℃ membrane only need one day, spray is 180～200 tails/g in the time of the 5th day, preferred the 5th afternoon or the 6th day morning are for sending out the optimum time of seedling.
The technology of the present invention has solved that production cost is higher in the yellow cartfish artificial propagation prior art, labour intensity is larger, and the production scale degree is not high; The facilities such as pond are had relatively high expectations, and the shortcoming such as climate and water quality impact be remarkable; Overcome yellow cartfish and laid eggs less at high temperature season, the problem of even producing has not improved fertilization rate, incubation rate and emergence rate, effectively reduces the difficult problem that the hatching process water mold infects, and has realized that low-intensity, low cost, the extensive fry of order formula produce.
Because the distinctive chrysanthemum shape of yellow cartfish milter spermary structure, cause manually and can not extrude sperm, take the biphasic injection oxytocic drug after, obtain sperm in " gametocide is got essence " mode, this mode is very unfavorable for the protection parent, parent's service efficiency is low.Because ovum is manually to extrude equally, the rate of development of ovum is also asynchronous, and therefore the fertilization rate of " gametocide is got essence " this mode is unstable, and amplitude of variation is larger, between 30～80%.Compare with " gametocide is got essence " yellow cartfish artificial propagation mode, the technology of the present invention is utilized the yellow cartfish spawning habit, and the fish nest is set, and hastening parturition by a shot reaches spawning effect.The parent reaches to oestrus after the effect time and chases mating, and the ovum 98% of therefore discharging is reached maturity, and fertilization rate can reach 82～92%, and fertilization rate increases substantially, and stable, has improved the quantity of emerging.Simultaneously change a shot into by biphasic injection, only need the second injection amount of injection biphasic injection method just can make spawning rate reach effect more than 90%, reduce shot parent's number of times, reduced the mechanical damage to the parent, also shortening simultaneously the parent supports the time in cement pit temporarily, save medicine, reduced labour intensity." gametocide is got essence " yellow cartfish artificial propagation mode step is various, needs the work staff more, and is consuming time longer, compares the parent propagation that carries out same quantity, and the inventive method has been simplified operating procedure, has reduced the labour, increases work efficiency.
Present another kind of artificial propagation method of pelteobagrus fulvdraco is yellow cartfish pond natural spawning mode, and this mode is utilized the yellow cartfish spawning habit of nesting, and places the fish nest mating season in the pond, regularly collects the breeding fish nest, and the palm sheet is hung on the feed-based hatchery hatching.This mode is not conflicted mutually with breeding production, produces longer duration, and the parent is injury-free, can repeatedly use, but but have following shortcoming, for example: 1. reach 400～5,000,000/batch the required parent's quantity of output to reach 15000kg, with 1875～2250kg/hm
2Breeding density calculates to be needed with pond 6.7～8hm
2, use parent and pond more, efficient is lower; 2. the yellow cartfish natural spawning is very strict to environmental requirement, and it is considerably less to reach more than 28 ℃ in water temperature, rain, lay eggs during plum rains, therefore can not provide a large amount of fries when high temperature needs fry to send out the pool; 3. owing to fish-egg density on the palm sheet differs, what have is very high, and the hatching process water mold is serious, and for preventing that water mold is diffused in hatching process and uses high-concentration formaldehyde to soak, medicine more or less exerts an influence to fry, and incubation rate is very low, usually between 20～30%.Compare with yellow cartfish pond natural spawning mode, the present invention has overcome the shortcoming of yellow cartfish natural spawning mode, and concrete clear superiority is mainly reflected in: (1) output forms scale, and the realization order is quantitatively produced.Same produce 400～5,000,000/batch fry, only need to use the about 340kg of parent, can get as required a certain amount of parent production fry of hastening parturition; (2) it is few to take the pond, and parent's service efficiency is high.Parent's breeding density of the present invention can reach 6000kg～7500kg/hm
2, even higher, improve the pond service efficiency; (3) overcome natural cause, still can be mass-produced at 30-33 ℃ high temperature season.Because the mode that adopts oxytocic drug to hasten parturition, the parent is through the mating of must laying eggs after the injection oxytocic drug, and it is less affected by extraneous natural cause, still can provide a large amount of fries in the time of therefore can not producing in the high temperature pond, satisfies need of production; (4) solve water mold and infect, improve incubation rate, reduce drug use.Significantly reduced the density of fish-egg on the palm sheet by washing the ovum step, do not used the medical treatment water mold, not having medicine that fish-egg is produced stimulates.Adopt the hatching of throwing cylinder to hatch the mode that combines with cement pit, incubation rate generally reaches more than 60%, even surpasses 80%.
Yellow cartfish propagation technique method of the present invention is simple to operate, the fish nest is easy to make, fish-egg is collected easily, parent's availability is high, can obtain single batch of output large, grow neat fry, especially still can normally breed the high temperature season yellow cartfish, not affected by the nature climate, guarantee low-cost a large amount of supplies of yellow cartfish seedling.In addition, adopt and wash the ovum mode and adopt the throwing cylinder to hatch, effectively reduce the infection of hatching process water mold, increase substantially emergence rate, reduce in the use of breeding the stage medicine, guaranteed the seed quality.
Additional aspect of the present invention and advantage in the following description part provide, and part will become obviously from the following description, or recognize by practice of the present invention.
Embodiment
Below in conjunction with embodiment embodiment of the present invention are described in detail, but it will be understood to those of skill in the art that the following example only is used for explanation the present invention, and should not be considered as limiting scope of the present invention.
Embodiment one
1. parent
1.1 parental source
The parent can select pond culture two age the parent, or the lake wild catch two age the parent.
1.2 parent's transportation
Parent's transportation keeps low-density, and water temperature is between 20～27 ℃, and the transportation water body can not add medicine, and operation is gently avoided the scratch of fish body when putting into the pond.
1.3 parent culture
The parent puts in a suitable place to breed in the pond with male and female proportioning 2～3: 1, density 6000kg～7500kg/hm
2Normally throw something and feed protein content at 〉=40% feed with 1～1.5% appetite when water temperature is higher than 12 ℃ before surviving the winter.Water temperature stops the feed of throwing something and feeding when being lower than 12 ℃, keep the depth of water 1.8～2.0m, water transparency≤30cm.The beginning of spring is later along with the temperature rises, when water temperature reaches more than 12 ℃, reduce water depth, keep the depth of water 1.0～1.2m, with 1% protein content≤38% feed of throwing something and feeding, cooperate the vitamin E of throwing something and feeding, Vc, new iced trash fish, Immune Yolk Antibody, promote gonad development, prevent that parent's intra abdominal fat from hoarding too much, the impact ovulation.Do not use medicine between breeding period as far as possible, therefore extremely important to the water quality management between breeding period, can only apply fertilizer and add and change water.
1.4 parent's specification
Mid or late May to by the end of August, when water temperature during at 24～31.5 ℃, begin to carry out the yellow cartfish artificial propagation.Specially support the pond the yellow cartfish parent and draw in the net, pick out more than the raun specification 75g/ tail, more than the milter specification 150g/ tail, select the male and female ratio and be 2～3: 1 parent.The raun belly is full, high resilience, and gonopore is obvious, and belly has sense of movement during handstand; The milter gonapophysis is obvious, is cerise, and anosis without hindering, fin ray is complete.
1.5 parent's cement pit is supported temporarily
The parent of select changes cement pit over to and supports temporarily, in order to hastening parturition injection.Cement pit breeding density≤25kg/m
3, evenly placement increases oxygen gas head.The feed owing to do not throw something and feed during supporting is temporarily eliminated the emergent impact that environmental change produces the parent again, and the time of therefore supporting temporarily is no less than 4h, is no more than 24h, and oversize parent of time is because environment space is narrow and small, the death of not taking food is more serious.
2. hasten parturition
2.1 inject time
Yellow cartfish lays eggs to be typically chosen in and carries out afternoon, and according to these characteristics, should carry out between 14:00～19:00 inject time, directly is put in the spawning pond after parent's injection.
2.2 injection system
Adopt thoracic cavity abdomeinal fin base portion one pin injection, needle tubing inserts about 0.5-1cm, and the about 1ml of injected dose specifically looks parent's specification and takes the circumstances into consideration increase and decrease.
2.2 injectable drug
Oxytocic drug can be selected hypophysis (PG) or Malaysia DOM DOM, luteotropin releasing hormone d-ala analog (LRH-A
2), human chorionic gonadotropin (HCG), used dosage is every kilogram of PG 3～5mg or DOM 3～5mg+LRH-A
220～30 μ g+HCG, 800～1200IU, the same dosage of male and female.
2.3 the fish nest is made and is placed
2.3.1 fish nest preparation method
At first selecting the plastic cylinder of high 25cm, internal diameter 30cm is the breeding fish nest, and color is better with black.Secondly at the full palm sheet of plastic cylinder bottom tiling, with the 8# galvanized wire hoop palm sheet that at the bottom of bucket, stretches tight, prevent that the parent from causing that when mating the palm sheet moves.The palm sheet must be washed dust and impurity off through being immersed in the water after cutting off the hard edge, just can use after the operation such as drying in tanning by the sun under the sun.
2.3.2 the fish nest is placed
Yellow cartfish does not have strict requirement for spawning pond, difformity, different area, diverse location all can, but the intensity of light has certain impact on laying eggs, light is darker, the effect of laying eggs is better.The cement pit of laying eggs adds water to 0.35m, with the fish nest fill water sequence keep flat sink to the pond at the bottom of, quantity is identical with milter mantissa, density with 6-8 only/m
2Place, after putting the fish nest and finishing, with 1～2/m
2Be evenly arranged and increase oxygen gas head, the cement pit of will laying eggs afterwards adds to the depth of water 0.6～0.8m.
2.4 the effect time
Between 23～25 ℃ of timeliness are seasonable, be generally 19～24h, along with the corresponding shortening of water temperature effect time.
3. get the fish nest and collect fish-egg
3.1 get the fish nest time
Selection of time in the morning 7:00～8:00 begins, 40.5～42h after the distance injection.
3.2 wash ovum
It is very crucial to wash the ovum time, is generally spending primitive gut latter stage between the tail dentification phase.Fish-egg is in cell quick division stage between primitive gut, washes intense stimulus that ovum produces this moment and may cause that fish-egg is grown and stop that fish-egg is nearer to the membrane time after the tail dentification phase, is unfavorable for the operation in later stage.
The dried up time of palm sheet in the taking-up fish nest is no more than 15min it is put to washing the ovum cement pit.Wash the dark about 0.3～0.4m of ovum pond muddy water, 1～2/m
2Evenly place and increase oxygen gas head, miniflow water.The palm sheet of will laying eggs when washing ovum rocks in clear water back and forth gently, utilize fish-egg in the impulse force of water fish-egg to be washed, until the fish-egg on the palm sheet is sparse, finish during negligible amounts, afterwards the palm sheet is hung on the feed-based hatchery yoke and hatch, and the fish-egg that washes is collected rinsing immediately put into the hatching of throwing cylinder.
3.3 parent's postnatal care
With postpartum the parent put to the parent and specially support the pond nursing, with drug disinfection sterilizations such as chlorine dioxide, and the feed of suitably throwing something and feeding, add Vc etc. and strengthen its physique.
4. hatching
For solving hatching process water mold problem, not using medicine that fish-egg is produced stimulates, and reducing medicine affects fish-egg, adopts and throws two kinds of hatching patterns that combine of cylinder hatching and cement pit hatching.
4.1 throw the cylinder hatching
4.1.1 throwing cylinder structure
Throw the cylinder hatching and adopt the stainless steel and iron leather of inverted conical inner wall smooth to do, the bottom is open-minded, high 1.4m, internal diameter 1.2m, the about 0.5m of volume
3The hatching process high-level reservoir provides stable source of water, water quality dissolved oxygen DO 〉=5mg/L, the bottom is with the flow water flowing of 5L/min, keeps throwing water circulation in the cylinder, and with the speed of 30L/min to the water storage barrel new water of annotating.
4.1.2 hatching density
With fish-egg with 60～800,000/m
3Density is put in throws in the cylinder.
4.1.3 brooding time
About 28～the 34h of hatching in throwing cylinder totally goes to its rinsing immediately cement pit and supports temporarily behind the fry demoulding.
4.1.4 fry is supported temporarily
With 8～100,000 tails/m
2Support temporarily in the mud of pond the depth of water 0.1～0.3m, dissolved oxygen DO 〉=5mg/L.Storage pond, holding pond miniflow water is with 1/m
2Evenly place and increase oxygen gas head.
4.2 cement pit hatching
The cement pit hatching pattern is with 23～27/m with the palm sheet after the fish-egg cleaning
2Hang on the cement pit yoke, with 3～4/m
2Placement increases oxygen gas head, miniflow water, the depth of water 0.4～0.5m.Behind the fry membrane palm sheet and yoke are taken out, sucking-off fry when treating that fry is high-visible, wherein the rinsing of foul impurity is removed, and supports temporarily with throwing the cylinder hatching that mode is identical supports temporarily, with 8～100,000 tails/m
2Support temporarily in the mud of pond.
5. fry sends out the pool
The rate that water temperature is grown the yellow cartfish fish-egg is larger, along with the rising fish-egg rate of development of water temperature is faster.Water temperature is in the time of 23～27 ℃, and the membrane time needs two days, and spray is 240～250 tails/g in the time of the 5th day; Membrane only needs one day when water temperature reaches more than 28～31.5 ℃, and the 5th day is 180～200 tails/g.The 5th afternoon or the 6th day morning, fry did not scatter in order to send out the optimum time of seedling, and still take self yolk as nutrient source, the seedling quality is good, Yi Famiao.And during by the 7th day, fry self yolk nutrient consumption totally need to begin place's circle's nutritional supplementation, and fry scatters, move about at Quan Chi, and the fry Quality Down, and send out the seedling operating difficulties.
Embodiment two:
Yellow cartfish artificial propagation in 2012 hasten parturition altogether parent 2290kg, the meter 7508 groups, wherein raun 976.5kg, milter 1313.5kg hasten parturition 8 batches altogether, 5,120 ten thousand tails of emerging.The mean spawning rate 84.7%, fertilization rate 88.4%, incubation rate 80.5%.Parent 2409kg, wherein raun 1005kg, milter 1404kg are used in the natural propagation test of soil pond altogether.Collect incubating oosperm every day on May 25 to August 16, except high mild climate variation is too very much not laid eggs, hatch altogether 60 batches, add up to 3,032 ten thousand tails of emerging.
Table 1.2012 year yellow cartfish artificial propagation situation list
Native pond natural propagation method efficient is very low as can be drawn from Table 1, and single batch can not form output.The yellow cartfish propagation method of our invention can singly be criticized and obtain nearly output of 1,000 ten thousand, and efficient is high, production that can real single fixed pattern, and maneuverability is not affected by natural cause.
Embodiment three:
On June 2nd, 2012, on June 11st, 2012, on June 21st, 2012 are from selecting the available parent of hastening parturition from the pond, raun specification 100g/ tail, milter specification 175g/ tail, adopt abdominal cavity pectoral fin base portion injection, carry out different frequency injections and the contrast of different oxytocic drugs to yellow cartfish spawning rate and fertilization rate.
The different frequency injections of table 2., different oxytocic drug are on the impact of yellow cartfish spawning rate and fertilization rate
As can be known from Table 2, a shot is close with the quadratic response effect, does not affect spawning rate and fertilization rate, adopts in addition Malaysia DOM DOM to replace hypophysis PG, reduces the oxytocic drug using dosage spawning rate and fertilization rate are not made a significant impact.Adopt thus shot mode, use every kilogram of DOM 3mg+LRH-A
230 μ g+HCG 800IU drug ratios can reach preferably spawning effect.Compare the biphasic injection mode and saved oxytocic drug, saved the parent and supported the time temporarily, reduced parent's loss, simplified operating procedure, reduced medicine the parent is produced negative interaction, increase work efficiency.
Embodiment four:
On June 29th, 2012, fine, 31.5 ℃ of temperature, 28 ℃ of water temperatures use yellow cartfish raun specification to be 150g/ tail 720 tails as 100g/ tail 1440 tails, milter specification, and male and female were than 2: 1, inject 1 pin, inject time 14:00～17:00, with 0.7% physiological saline preparation liquid medicine injection, oxytocic drug dosage is every kilogram of DOM 3mg+LRH-A
230 μ g+HCG 800IU are with outdoor square cement pool area 15m
2, 25mm
2, indoor shape cement pit area 15m
2, 7.5m
2, indoor circular cement pit 20m
2As spawning pond, begin to chase after the feelings mate and oviposit behind the 20h, the end of laying eggs behind about 7h, 8:00 on the 3rd begins 10:00 and finishes to get the fish nest, washes ovum, hangs the palm sheet.Fish-egg is put into the hatching of throwing cylinder, and 14:00 will throw the rinsing of nautilus seedling on 4, with 8～100,000/m
2Support in cement pit temporarily.Feed-based hatchery was got brown sheet on the 4th, and clingfish seedling on the 5th is removed foul impurity, with 8～100,000/m
2Support temporarily in cement pit, 8:00 sent out the pool on 6, obtained fry 5,340,000 tails.
Table 3. different egg environment is on the impact of spawning rate
As can be drawn from Table 3, spawning pond size, spawning pond position have no significant effect the yellow cartfish spawning rate, and the intensity of light is more remarkable on the impact of spawning rate, and spawning rate was lower when light intensity was strong, and spawning rate was higher when light intensity was weak.
Embodiment five:
Select that yellow cartfish parent raun specification 80g/ tail, milter specification 175g/ tail have carried out different inject times under identical egg-laying environment, difference is washed the ovum time to the impact of yellow cartfish reproductive effect on August 6th, 2012, on August 7th, 2012, on August 10th, 2012.
Table 4. different inject times with wash the ovum time to the impact of yellow cartfish reproductive effect
As known from Table 4, different inject times are not remarkable on spawning rate, fertilization rate impact, and incubation rate is had appreciable impact.From on August 6th, 2012 with Augusts 10 in 2012 the twice-daily result that hastens parturition can draw, it is identical to wash the ovum time, inject time, the former was afternoon, the latter is the morning, the former ovum rate is 84.3%, the latter is 59.6%, the former is apparently higher than the latter.Might be to conform to the yellow cartfish spawning habit owing to the former injects the rear excessively effect time, latter's difference causes.On August 6th, 2012 with Augusts 7 in 2012 the twice-daily result that hastens parturition compare, the former washes the ovum time and is in the primitive gut latter stage that fish-egg is grown, it's the tail dentification phase has past the latter, therefore cause incubation rate not identical, therefore can draw between the primitive gut latter stage and tail dentification phase of washing the most well fish-egg growth of ovum time.Combining rear three spawning effects contrast can draw, and the yellow cartfish artificial propagation is hastened parturition inject time to be advisable afternoon, washes the ovum time to pass between the primitive gut latter stage and tail dentification phase that fish-egg grows.
More than about specific descriptions of the present invention, only for the present invention being described and being not to be subject to the technical scheme that embodiment of the invention rope is described, those of ordinary skill in the art should be appreciated that still and can make amendment or be equal to replacement the present invention, reached constructed effect; Use needs as long as satisfy, all within protection scope of the present invention.
Claims (10)
1. a yellow cartfish artificial propagation technique is characterized in that, may further comprise the steps:
A, parent culture: select the yellow cartfish parent by male and female proportioning 2～3: 1 combo;
B, oxytocic drug injection: carry out 1 oxytocic drug injection;
C, fish nest and placement quantity: put into spawning pond after the injection, the fish nest is identical with male parent mantissa in the spawning pond, and parent's mating is originated in fertilized egg in the fish nest;
D, get the fish nest and wash ovum: lay eggs and the fish nest is taken out after finishing, the palm sheet in the fish nest is carried out rinsing in clear water, the fish-egg that washes is collected;
E, hatching and foster temporarily: the fish-egg that will wash collection is put in throws hatching in the cylinder, be put in behind the fry membrane in the cement pit and support temporarily, palm sheet after simultaneously fish-egg being cleaned hangs on the cement pit yoke hatches, and behind the fry membrane palm sheet and yoke is taken out, and supports temporarily in cement pit;
F, a pool time: treat the complete taking-up of fry membrane palm sheet in the hatchery, clean, continue to be put in to support temporarily in the cement pit and treat a pool.
2. yellow cartfish artificial propagation technique according to claim 1 is characterized in that in parent culture, the parent is with 6000～7500kg/hm in the steps A
2Put in a suitable place to breed, the front water temperature of surviving the winter is thrown something and fed 〉=40% protein feed with 1～1.5% amount more than 12 ℃ the time, the depth of water 1.8～2.0m, transparency is not less than 30cm, be adjusted into 1.0～1.2m with the depth of water spring, water temperature begins the feed of throwing something and feeding after being higher than 12 ℃, admixes vitamin E, Vc and immunity additive in feed, with the throw something and feed feed of albumen≤38% of 1% amount.
3. yellow cartfish artificial propagation technique according to claim 1 is characterized in that, in the oxytocic drug injection, adopts 1 injection of abdominal cavity pectoral fin among the step B, and oxytocic species amount and dosage are every kilogram of PG3～5mg or DOM3～5mg+LRH-A
210～30 μ g/+HCG800～1200IU, needle tubing inserts the about 0.5-1cm of pectoral fin base portion, and every tail is injected the 1ml liquid approximately, the same dosage of female milter.
4. yellow cartfish artificial propagation technique according to claim 1, it is characterized in that, when the fish nest is made with placement among the step C, it is the breeding fish nest that the fish nest is selected the black plastic drum of high 25cm, internal diameter 30cm, the palm sheet is placed in the bottom, the fish nest lies in the cement pit of laying eggs, and quantity is identical with milter parent mantissa, density is 6-8/m
2Place.
5. yellow cartfish artificial propagation technique according to claim 1 is characterized in that, the cement pit depth of water of laying eggs among the step C 0.6～0.8m, and female milter proportioning is 2～3: 1.
6. yellow cartfish artificial propagation technique according to claim 1, it is characterized in that, get the fish nest among the step D and wash the ovum time, getting the fish nest time is 40.5～42h after the yellow cartfish injection, the dried up time of palm sheet is no more than 15min, wash the ovum time the primitive gut latter stage that crosses that fish-egg grows between the tail dentification phase, will be placed with clear water, increase in the cement pit of oxygen gas head and miniflow water and clean with the palm sheet of fish-egg.
7. yellow cartfish artificial propagation technique according to claim 1 is characterized in that, hatching is when supporting temporarily in the step e, and hatching is adopted and thrown the cylinder hatching and hatch the mode that combines with cement pit.
8. yellow cartfish artificial propagation technique according to claim 1 is characterized in that, in the step e with fish-egg with 60～800,000/m
3Density is thrown in wherein, and 28～34h is supported in water hatching that the bottom is through-flow temporarily, and is behind the fry demoulding that its rinsing is clean, with 8～100,000 tails/m
2Support temporarily in the mud of pond, the depth of water 0.1～0.3m, dissolved oxygen DO 〉=5mg/L sends out the pool after supporting temporarily 40～64h during 24～29 ℃ of water temperatures; Palm sheet after egg white is washed in cement pit hatching employing is with 23～27/m
2Hang on the cement pit yoke, place and to increase oxygen gas head, miniflow water, the depth of water 0.4～0.5m behind the fry membrane takes out palm sheet and yoke, and sucking-off fry when treating that fry is high-visible is with foul impurity rinsing removal, with 8～100,000 tails/m
2Support temporarily in the mud of pond.
9. yellow cartfish artificial propagation technique according to claim 1 is characterized in that, sends out the pool time in the step F, and water temperature is in the time of 23～27 ℃, and the membrane time needs two days, and spray is 240～250 tails/g in the time of the 5th day; Or water temperature when reaching more than 28～31.5 ℃ membrane only need one day, the 5th day is 180～200 tails/g.
10. yellow cartfish artificial propagation technique according to claim 1 is characterized in that, the 5th afternoon or the 6th day morning are for sending out the optimum time of seedling in the step F.
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| CN103478032A (en) * | 2013-09-17 | 2014-01-01 | 中国水产科学研究院珠江水产研究所 | Natural spawning and hatching culture method for silver arowana |
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| CN107646757A (en) * | 2017-10-18 | 2018-02-02 | 安徽省农业科学院水产研究所 | A kind of fish laying nest and its application in the Yellow River carp breeds |
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| CN103478032A (en) * | 2013-09-17 | 2014-01-01 | 中国水产科学研究院珠江水产研究所 | Natural spawning and hatching culture method for silver arowana |
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| CN105409851A (en) * | 2015-12-15 | 2016-03-23 | 黄伟 | Feeding method for yellow catfishes |
| CN107646757A (en) * | 2017-10-18 | 2018-02-02 | 安徽省农业科学院水产研究所 | A kind of fish laying nest and its application in the Yellow River carp breeds |
| CN109349171A (en) * | 2018-11-30 | 2019-02-19 | 河南省水产科学研究院 | A kind of quasi- Chang Ecology propagation method |
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