CN102960314B - Device and method for studying phototactic behavior characteristics of inserts - Google Patents
Device and method for studying phototactic behavior characteristics of inserts Download PDFInfo
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- CN102960314B CN102960314B CN201210550196.2A CN201210550196A CN102960314B CN 102960314 B CN102960314 B CN 102960314B CN 201210550196 A CN201210550196 A CN 201210550196A CN 102960314 B CN102960314 B CN 102960314B
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Abstract
The application discloses a device and a method for studying phototactic behavior characteristics of inserts. The device comprises an insert cultivation device, semi-conductor light sources, a video collection module, and a processor, wherein the insert cultivation device comprises a plurality of observation rooms, and each room is equipped with a semi-conductor light source with adjustable wavelength and lighting intensity, so that the semi-conductor light source can give off lights in needed wavelength and lighting intensity directly for the observation room, improving the accuracy of study results. In addition, in the technical solution provided by the embodiment of the invention, the video collection module arranged at a second through hole to collect video images of insert behavior enables real-time observation at 360 degrees on the insert in each observation room, so that insert behavior under different optical spectrums and lighting intensities can be obtained without interruption in a real-time manner, providing reliable data for analyzing insert behavior under different optical spectrums and lighting intensities.
Description
Technical field
The present invention relates to agricultural entomology research field, relate in particular to a kind of become device and method of light behavioral trait of insect of studying.
Background technology
Phototaxis is one of important Ecological Characteristics of numerous night flight or navigation insects.In < < China agricultural encyclopedia > > insect roller, the phototaxis of insect is classified as to the taxis category of insect, being the selectivity of insect to environmental condition, is also the instinct that insect forms in the process that conforms for a long time.Because the phototaxis of insect has very consequence and effect in prediction, the forecast of insect and in preventing and treating, recent decades, Chinese scholars is with regard to the phototaxis and different spectrum, the relation between intensity of illumination of insect, the phototactic physiological foundation of insect, and all many-sides such as the phototactic mechanism of insect, essence have been carried out extensive and deep research.
Yet during being related between the phototaxis of studying insect in prior art and different spectrum, intensity of illumination, experimental provision and method precision are lower, cause result of study inaccurate.
Summary of the invention
For solving the problems of the technologies described above, the embodiment of the present invention provides a kind of become apparatus and method of light behavioral trait of insect of studying, when overcoming being related between the phototaxis of studying insect in prior art and different spectrum, intensity of illumination, experimental provision and the lower problem of method precision, the accuracy of raising result of study.
For addressing the above problem, the embodiment of the present invention provides following technical scheme:
Study the become device of light behavioral trait of insect, comprising:
Insect breeding device, described insect breeding device is gas permeability device, comprise: mutually nested and light tight inner casing that two ends are concordant and light tight shell, the light tight top cover that covers described inner casing and shell two ends completely and light tight bottom and the annular space that described inner casing, shell, top cover and bottom are formed are divided into the light tight division board of a plurality of observation ward, wherein, between described inner casing lower end and described each observation ward, and between described division board and bottom, have for insect first through hole that shuttles back and forth, between described division board and top cover, there is the second through hole;
Be arranged on wavelength and the adjustable semiconductor light sources of intensity of illumination in each observation ward;
Be arranged on the second through hole for the video acquisition module of the motion video image that entomologizes;
Control wavelength and/or the intensity of illumination of described semiconductor light sources, and the processor that obtains and analyze described video acquisition module information.
Preferably, described processor comprises:
Be connected with described semiconductor light sources, regulate described semiconductor light sources, make the intensity of illumination first processor consistent with default intensity of illumination of tested point in each observation ward;
Be connected with described video acquisition module, the sequence of video images of extraction activity insect the insect activity video image gathering from described video acquisition module, and according to the sequence of video images of described movable insect and the state parameter of the Insects crawler behavior obtaining in advance set up HMM, and according to HMM, obtain the second processor of insect behavior state sequence.
Preferably, also comprise:
Be connected with described processor, record the memory of insect behavior state sequence.
Preferably, described memory comprises:
Record the first memory of radical response insect behavior state sequence and normal reaction insect behavior state sequence;
Record insect current behavior status switch respectively with described first memory in the second memory of comparative result of radical response insect behavior state sequence and normal reaction insect behavior state sequence.
Preferably, also comprise:
Be connected with described video acquisition module, the insect activity video image of described video acquisition module collection be uploaded to the communication device of remote data center.
Preferably, described semiconductor light sources comprises:
Be used to plant that luminous energy is provided, the first semiconductor light sources that it can be grown in enclosure space; With,
At least one is for luring the second semiconductor light sources of worm and/or driving.
Preferably, described insect breeding device also comprises: be arranged at the fluorescence cloth on described side wall of outer shell and described division board sidewall.
Preferably, described insect breeding device also comprises: be opened in feeding port on described shell and for sealing the shutter of described feeding port.
Study the become method of light behavioral trait of insect, be applied to device described in claim 1, comprising:
Intensity of illumination or the wavelength of semiconductor light sources in default each observation ward;
Insect to be studied is positioned in the internal chamber of described inner casing, so that described insect to be studied is shuttled back and forth to each observation ward by described the first through hole;
Open video acquisition module, so that described video acquisition module is captured in the video image of the insect activity in each observation ward;
Control intensity of illumination or the wavelength of described semiconductor light sources, and the image information that described video acquisition module is collected is analyzed.
Preferably, intensity of illumination or the wavelength of controlling described semiconductor light sources comprise:
Regulate described semiconductor light sources, make the intensity of illumination of tested point in each observation ward consistent with default intensity of illumination.
Preferably, the image information analysis described video acquisition module being collected comprises:
The sequence of video images of extraction activity insect from the insect activity video image of described video acquisition module Real-time Collection;
According to the state parameter of the sequence of video images of described movable insect and default Insects crawler behavior, set up HMM;
According to HMM, obtain insect current behavior status switch.
Preferably, the state parameter of described default Insects crawler behavior calculates according to insect activity cybernetics control number in insect breeding device.
Preferably, also comprise:
Pre-stored radical response insect behavior state sequence and normal reaction insect behavior state sequence;
Record insect current behavior status switch respectively with the comparative result of described radical response insect behavior state sequence and normal reaction insect behavior state sequence.
Preferably, also comprise: the insect activity video image of described video acquisition module collection is uploaded to remote data center.
Compared with prior art, technique scheme has the following advantages:
In the technical scheme that the embodiment of the present invention provides, insect breeding device comprises a plurality of observation ward, and in each observation ward, be provided with the adjustable semiconductor light sources of wavelength and intensity of illumination, thereby can make the semiconductor light sources in each observation ward, directly send the light of required wavelength in each observation ward or intensity of illumination, solve in prior art scheme, utilization is filtered artificial light source and is regulated, while obtaining required wavelength or intensity, the problem that precision is lower, has improved the accuracy of result of study.And, in the technical scheme that the embodiment of the present invention provides, be arranged on the second through hole for the video acquisition module of the motion video image that entomologizes, can carry out 360 ° of Real Time Observation to the insect in each observation ward, thereby can be continuously or obtain in real time the behavior of insect under different spectrum, intensity of illumination, when analyzing the behavior of insect under different spectrum and intensity of illumination, reliable data are provided.
Accompanying drawing explanation
The become structural representation of device of light behavioral trait of the research insect that Fig. 1 provides for the embodiment of the present invention one;
The structural representation of the insect breeding device that Fig. 2 provides for the embodiment of the present invention one;
The become schematic flow sheet of method of light behavioral trait of the research insect that Fig. 3 provides for the embodiment of the present invention two;
The become schematic flow sheet of method of light behavioral trait of the research insect that Fig. 4 provides for the embodiment of the present invention three.
Embodiment
Just as described in the background section, during being related between the phototaxis of studying insect in prior art and different spectrum, intensity of illumination, experimental provision and method precision are lower, cause result of study inaccurate.
Inventor studies discovery, and during being related between the phototaxis of studying insect in prior art and different spectrum, intensity of illumination, the artificial light source in experimental provision is mainly incandescent lamp or fluorescent lamp.And when utilizing being related between this light source research phototaxis of insect and the light source of different spectrum, intensity of illumination, typically use the net sheet of different colours, or the light path being comprised of mirror group far away, interferometric filter, neutral filter, speculum, frosted glass etc. filters to this light source, thereby obtain the spectrum of required wavelength, and by adjustable transformer, regulate the intensity of light source of artificial light source.
Yet the degree of regulation of incandescent lamp or fluorescent light source strength is inadequate, make leached spectral width in 30nm left and right, make experimental technique precision of the prior art lower, cause result of study inaccurate.And, by adjusting transformer, regulating in the intensity of light source process of this artificial light source, the spectrum of light source can be offset, and also can make experimental technique precision of the prior art lower, thereby further reduces the accuracy of result of study.
In addition, experimental technique of the prior art, behavior to insect under different spectrum, intensity of illumination lacks continuous or real-time observation, causes, when analyzing the behavior of insect under different spectrum and intensity of illumination, lacking reliable data.
In view of this, the invention provides a kind of become apparatus and method of light behavioral trait of insect of studying, when overcoming being related between the phototaxis of studying insect in prior art and different spectrum, intensity of illumination, experimental provision and the lower problem of method precision, improve the accuracy of result of study.
For above-mentioned purpose of the present invention, feature and advantage can more be become apparent, below in conjunction with accompanying drawing, the specific embodiment of the present invention is described in detail.
Set forth detail in the following description so that fully understand the present invention.But the present invention can be different from alternate manner described here and implements with multiple, and those skilled in the art can do similar popularization without prejudice to intension of the present invention in the situation that.Therefore the present invention is not subject to the restriction of following public concrete enforcement.
Embodiment mono-:
As shown in Figure 1, the embodiment of the present invention provides a kind of become device of light behavioral trait of insect of studying, and comprising:
Insect breeding device, described insect breeding device is gas permeability device, as shown in Figure 2, comprise: mutually nested and light tight inner casing 101 that two ends are concordant and light tight shell 102, the light tight top cover 103 that covers described inner casing 101 and shell 102 two ends completely and light tight bottom 104 and the annular space that described inner casing 101, shell 102, top cover 103 and bottom 104 are formed are divided into the light tight division board 105 of a plurality of observation ward, in order clearly to show demarcation strip 105, in Fig. 2 by demarcation strip 105 blackings.Wherein, between described inner casing 101 lower ends and described each observation ward, and between described division board 105 and bottom 104, have for the insect first through hole (not shown) of shuttling back and forth, between described division board and top cover, there is the second through hole (not shown); And in one embodiment of the invention, the height of described the first through hole on along described bottom 104 to top cover 103 directions is preferably 10 ㎝, and the height of described the second through hole on along described bottom 104 to top cover 103 directions is preferably 20 ㎝ but the present invention does not limit this.
Because the material of inner casing 101, shell 102, top cover 103, bottom 104 and the division board 105 of described insect breeding device is light-proof material, therefore, described insect breeding device is an airtight darkroom.When concrete application, in the internal chamber of described inner casing 101, be placed with insect to be studied, in each observation ward, place the plant that promising insect to be studied provides habitat, and between described inner casing 101 lower ends and described each observation ward, and between described division board 105 and bottom 104, have for insect first through hole that shuttles back and forth, thereby make insect to be studied can be between the internal chamber of described insect breeding device and each observation ward free shuttling.
In one embodiment of the invention, the structural framing of described insect breeding device is spliced to form by steel pipe or aluminium-alloy pipe or PVC material pipe, and the remainder in the structural framing of described insect breeding device consists of opaque material.For guaranteeing that described insect breeding device is gas permeability device, in one embodiment of the invention, described shell 102 can be air-permeable structure, as the black gauze of top cover 103 and bottom 104 peripheries as described in being fixed on, also can be the overall structure partly being formed by gas permeability part and air impermeability, in another embodiment of the present invention, described top cover 102 can be air-permeable structure, as the black gauze on shell 102 tops as described in being fixed on, in other embodiments of the invention, also can be air-permeable structure for the miscellaneous part in described insect breeding device, the present invention does not limit this, as long as can guarantee described insect breeding device and external environment, can carry out air exchange.
In one embodiment of the invention, described insect breeding device is preferably six prismatics, comprise Liu Ge observation ward, wherein, the vertical view of described shell 102 is preferably the regular hexagon of the length of side 160 ㎝, the vertical view of described inner casing 101 is preferably the regular hexagon of the length of side 60 ㎝, described bottom 104 is preferably 200 ㎝ to the distance between top cover 103, in other embodiments of the invention, described insect breeding device can also be for cylindrical, seven is prismatic, eight is prismatic etc., the quantity of the observation ward that it is included can be seven, eight etc., the present invention does not limit this, depending on concrete Research Requirements.
In one embodiment of the invention, described bottom 104 is movable, can open and close, convenient food and the water of adding, in another embodiment of the present invention, described insect breeding device also comprises: be opened in feeding port on described shell 102 and for sealing the shutter of described feeding port, to guaranteeing in the lighttight situation of described insect breeding device, be convenient for changing food and water in described insect breeding device, to guarantee the food supply of insect to be studied.
Be arranged on wavelength and the adjustable semiconductor light sources of intensity of illumination in each observation ward, the wave-length coverage of described semiconductor light sources is between 200nm-700nm;
Described semiconductor light sources comprises and is used to plant that luminous energy is provided, and make its first semiconductor light sources that can grow in enclosure space, and at least one is for luring the second semiconductor light sources of worm and/or driving.Because insect to be studied can be freely movable in the internal chamber of described insect breeding device and each observation ward, so be all placed with the plant perching for insect to be studied in the internal chamber of described insect breeding device and each observation ward, in the internal chamber of corresponding described insect breeding device and each observation ward, be provided with as plant provides luminous energy the first semiconductor light sources that it can be grown in enclosure space.For the ease of studying the phototaxis of insect to be studied, in each observation ward of described insect breeding device, be provided with the second semiconductor light sources, the wavelength of the second semiconductor light sources described in each observation ward or intensity of illumination are different, and the second semiconductor light sources in each observation ward is isolated mutually, to avoid the cross influence of the second semiconductor light sources in adjacent observation ward.
It should be noted that, for the second semiconductor light sources and the second semiconductor light sources for driving that lures worm, its spectral composition composition is not identical, in one embodiment of the invention, described semiconductor light sources can comprise that at least one is for luring the second semiconductor light sources of worm, in another embodiment of the present invention, described semiconductor light sources can comprise that at least one is for the second semiconductor light sources of expelling parasite, in the third embodiment of the present invention, described semiconductor light sources can comprise that at least one is for luring the second semiconductor light sources of worm and driving, the present invention does not limit this.
In one embodiment of the invention, the photochromic of described the second semiconductor light sources can be warm white (colour temperature 3200K-6000K and 3200K following), positive white light (6000K-6500K) or cold white light (6500K-7000K and more than 7000K), purple light (370nm-420nm), blue light (420nm-500nm), green glow (500nm-560nm), gold-tinted (560nm-590nm), orange light (59lnm-610nm), six kinds of colors of ruddiness (620nm-700nm).Described the first semiconductor light sources is comprised of the semiconductor light sources of red, blue two kinds of wavelength.In embodiments of the present invention, described the second semiconductor light sources, can directly send the light of required wavelength in each observation ward or intensity of illumination, thereby solved in prior art scheme, utilization is filtered artificial light source and is regulated, while obtaining required wavelength or intensity, the problem that precision is lower, has improved the accuracy of result of study.
Preferably, described semiconductor light sources can comprise diode, and described diode is preferably light emitting diode or laser diode, and wherein light emitting diode includes OLED and inorganic light-emitting diode.
It should be noted that, in one embodiment of the invention, described insect breeding device also comprises: be arranged at the fluorescence cloth on described shell 102 sidewalls and described division board 105 sidewalls, so that the illumination patterns in described observation ward is more even.Preferably, the fluorescence cloth being arranged on described shell 102 sidewalls is totally enclosed type, and described insect breeding device and external environment are isolated completely, and size is 160 ㎝ * 180 ㎝.
Be arranged on the second through hole for the video acquisition module of the motion video image that entomologizes;
Because described video acquisition module is arranged on described the second through hole, and described the second through hole is arranged between division board 105 and top cover 103, therefore, each video acquisition module all can collect in adjacent Liang Ge observation ward, the active situation of insect to be studied, thereby device provided by the present invention can be realized 360 of insect to be studied ° of continuous, Real Time Observation, and then for the behavior of research insect to be studied under different spectrum or intensity of illumination, provide reliable data.
In one embodiment of the invention, described video acquisition module can be camera, is preferably OV7620 camera, and OV7620 camera is the integrated and high-resolution cmos image sensor of a height, support YCbCr and the output of RGB data format, resolution is 640 * 480.
The programmable functions register of OV7620 camera inside is provided with power mode and SCCB programming mode.The embodiment of the present invention can adopt SCCB programming mode, and SCCB programming mode can 8 RGB data outputs of continuous sweep.SCCB is the I2C agreement of simplifying, and SIO-0 is serial bidirectional data line, and SIO-1 is serial clock input line, is equivalent to SCL and SDA in I2C agreement.SCCB bus timing and I2C are basic identical, and the 9th of a transmission unit is its response signal ACK, are divided into NA position and Don ' t care position.NA position is produced by main frame, and Don ' t care position is produced by slave, because SCCB does not support that NA position must keep high level to multibyte continuous read-write.In addition, do not repeat initial concept in SCCB, therefore at SCCB, in the read cycle, in main frame sends sheet, after the address of register, must send bus stop condition, while not so sending read command, slave can not correctly produce Don ' t care response signal.
Control wavelength and/or the intensity of illumination of described semiconductor light sources, and the processor that obtains and analyze described video acquisition module information.
In one embodiment of the invention, described processor comprises:
Be connected with described semiconductor light sources, regulate described semiconductor light sources, make the intensity of illumination first processor consistent with default intensity of illumination of tested point in each observation ward;
Be connected with described video acquisition module, the sequence of video images of extraction activity insect the insect activity video image gathering from described video acquisition module, and according to the sequence of video images of described movable insect and the state parameter of the Insects crawler behavior obtaining in advance set up HMM, and according to HMM, obtain the second processor of insect behavior state sequence.
Preferably, described first processor comprises the light source driver module being connected with described semiconductor light sources, the embedded processing module being connected with described light source driver module, and the intensity of illumination sensing module being connected with described embedded processing module.
Wherein, intelligent light application time and the intensity of illumination of controlling described the second semiconductor light sources of described embedded processing module, and by described light source driver module, described the second semiconductor light sources is driven.Concrete, described embedded processing module is by controlling described the second semiconductor light sources, optical frequency in a periodicity of illumination is dodged the time, realizes the variation of intensity of illumination during different light pulse weeks, thereby utilizes optical pulse irradiation research to affect light pulse for the Diapause of insect.
Described intensity of illumination sensing module can move up and down measure the height of insect to be studied and its intensity of illumination in the plane, and determination data is uploaded to embedded processing module, when the height of insect to be studied and its institute's intensity of illumination are in the plane when inconsistent with setting illumination intensity value, automatically adjust dutycycle and the pwm signal of described light source driver module, thereby make its intensity of illumination reach setting value.
Preferably, described first processor and described the second processor can be 8 ATm ega16 single-chip microcomputers of high-performance, low-power consumption, and this single-chip microcomputer has following features: the In-System Programmable Flash of 16k byte (having the ability reading while write); 512 byte EEPROM; 1k byte SRAM; 32 universaling I/O port lines; 32 general purpose working registers, for the jtag interface of boundary scan, debugging and programming, 3 timer/counters flexibly with comparison pattern in supporting pieces; Serial line interface able to programme; Low-power consumption free time and power-down mode etc., support C Programming with Pascal Language.Built-in analog-to-digital conversion can be saved outside analog to digital conversion circuit when connecting external circuit simultaneously.Program of lower computer required data collection, conversion and with the functions such as communication of host computer.Program should comprise main program initialization, trace routine, measurement subprogram and serial communication programmer.Host computer requires the interface of realizing man-machine interaction, building database.The built-in RC oscillator circuit of ATm ega16 can produce 1M, 2M, and 4M, the frequency of oscillation of 8M, but generally use external crystal-controlled oscillation circuit in the occasion of having relatively high expectations.Y1 is quartz oscillator, and C15 and C16 are two electric capacity that capacitance equates, single-chip microcomputer employing+5V power voltage supply, and at P7 mouth access pull-up resistor and a luminous diode, play power supply indicative function.
Adopt ATm ega16 microprocessor, in conjunction with video acquisition module, the behavior of real-time monitored insect to be studied under different wavelengths of light; Adopt functional module mode to carry out the design of soft and hardware, measurement data can be sent to single-chip microcomputer by serial communication.
In one embodiment of the invention, the become device of light behavioral trait of described research insect also comprises: be connected with described processor, record the memory of insect behavior state sequence.Preferably, described memory comprises: the first memory that records radical response insect behavior state sequence and normal reaction insect behavior state sequence; With record insect current behavior status switch respectively with described first memory in the second memory of comparative result of radical response insect behavior state sequence and normal reaction insect behavior state sequence.
In another embodiment of the present invention, the become device of light behavioral trait of described research insect also comprises: be connected with described video acquisition module, the insect activity video image of described video acquisition module collection be uploaded to the communication device of remote data center.Preferably, described communication device can pass through wired or wireless network, the insect activity video image of described video acquisition module collection is uploaded to remote data center, thereby can be at remote data center, collected insect activity video image is analyzed, obtained insect current behavior status switch.
It should be noted that, described communication device not only can carry out real-time video transmission at certain single network access module, also can be any two kinds of combination of network patterns (being mainly WLAN/WCDMA, CDMA2000TD-SCDMA and WLAN/GPRS TD-LTE network), or carry out transmission of video under four kinds of combination of network mode condition, to obtain best transmission of video effect.
In sum, the research insect that the embodiment of the present invention provides becomes in the device of light behavioral trait, insect breeding device comprises a plurality of observation ward, and in each observation ward, be provided with the adjustable semiconductor light sources of wavelength and intensity of illumination, thereby can make the semiconductor light sources in each observation ward, directly send the light of required wavelength in each observation ward or intensity of illumination, solved in prior art scheme, utilization is filtered artificial light source and is regulated, while obtaining required wavelength or intensity, the problem that precision is lower, has improved the accuracy of result of study.And, in the technical scheme that the embodiment of the present invention provides, be arranged on the second through hole for the video acquisition module of the motion video image that entomologizes, can carry out 360 ° of Real Time Observation to the insect in each observation ward, thereby can be continuously or obtain in real time the behavior of insect under different spectrum, intensity of illumination, when analyzing the behavior of insect under different spectrum and intensity of illumination, reliable data are provided.
Embodiment bis-:
The become method of light behavioral trait of the device research insect providing in a kind of embodiment of utilization mono-is provided, as shown in Figure 3, comprises:
Step 101: intensity of illumination or the wavelength of semiconductor light sources in default each observation ward: the intensity of illumination of the second semiconductor light sources in each observation ward is fixed as to same intensity of illumination, the wavelength of semiconductor light sources in default each observation ward, make photochromic redness, yellow, green, blueness, purple and the white of being respectively of the second semiconductor light sources in each observation ward, as long as described the first semiconductor light sources can be used to plant that luminous energy is provided, it can be grown in enclosure space, and the present invention does not limit this.
Step 102: fangle and the water of identical and equivalent are placed respectively in same position in the internal chamber of described inner casing and each observation ward, and regularly supplement.Insect to be studied is placed in the internal chamber of described inner casing, so that described insect to be studied shuttles back and forth to each observation ward by described the first through hole, it is freely movable to be allowed to condition between the internal chamber of described inner casing and each observation ward.
Step 103: open video acquisition module, utilize described video acquisition module to monitor the active situation of insect to be studied in each observation ward, so that described video acquisition module is captured in the video image of the insect activity in each observation ward, and collected video image is uploaded to the second processor in host computer (calculator, panel computer).
Step 104: control intensity of illumination or the wavelength of described semiconductor light sources, and the image information that described video acquisition module is collected analyzes, obtain the light hobby characteristic of insect to be studied and the result of the behavioral trait under various wavelength light.Wherein, the content that the image information that described video acquisition module is collected is analyzed comprises: the time that described the second semiconductor light sources stops in different light compartment, the insect to be studied number of times that shuttles back and forth between different light compartment, the situation of the illumination compartment that insect to be studied is selected in bed, insect to be studied is the selection situation to illumination compartment when sucking, insect mating to be studied and the situation of laying eggs, insect to be studied at the movement locus of different light compartment, the stop place of plant etc.
Because the wavelength of the second semiconductor light sources in each observation ward is set, in one embodiment of the invention, intensity of illumination or the wavelength of controlling described semiconductor light sources comprise:
Step 10401: provide driving signal for described the second semiconductor light sources by the light source driver module in first processor;
Step 10402: utilize intelligent light application time and the intensity of illumination of controlling described the second semiconductor light sources of embedded processing module in first processor, be specially: by controlling described the second semiconductor light sources, optical frequency in a periodicity of illumination is dodged the time, realize the variation of intensity of illumination during different light pulse weeks, thereby utilize optical pulse irradiation research to affect light pulse for the Diapause of insect;
Step 10403: utilize intensity of illumination sensing module in first processor to move up and down to measure the height of insect to be studied and its intensity of illumination in the plane, and determination data is uploaded to embedded processing module, when the height of insect to be studied and its institute's intensity of illumination are in the plane when inconsistent with setting illumination intensity value, automatically adjust dutycycle and the pwm signal of described light source driver module, thereby make its intensity of illumination reach setting value.
The image information analysis in the embodiment of the present invention, described video acquisition module being collected comprises:
Step 10411: the sequence of video images of extraction activity insect from the insect activity video image of described video acquisition module Real-time Collection;
Step 10412: according to the state parameter of the sequence of video images of described movable insect and default Insects crawler behavior, set up HMM;
Step 10413: according to HMM, obtain insect current behavior status switch.
In one embodiment of the invention, the state parameter of described default Insects crawler behavior calculates according to insect activity cybernetics control number in insect breeding device, comprising: the crawler behavior characteristic parameter that defines insect to be studied in described insect breeding device; By the crawler behavior characteristic parameter of insect to be studied, by the method for data fusion, calculate the state parameter of a plurality of Insects crawler behaviors, as the behavior of searching for food, mating behavior and the behavior etc. of laying eggs.
In one embodiment of the invention, the become method of light behavioral trait of the research insect that the embodiment of the present invention provides also comprises:
Pre-stored radical response insect behavior state sequence and normal reaction insect behavior state sequence;
Insect current behavior status switch to be studied is compared with described radical response insect behavior state sequence and normal reaction insect behavior state sequence respectively, and record insect current behavior status switch to be studied respectively with the comparative result of described radical response insect behavior state sequence and normal reaction insect behavior state sequence.
Wherein, described pre-stored radical response insect behavior state sequence and normal reaction insect behavior state sequence comprise:
In described insect breeding device, place normal insect, and be allowed to condition at normal life in described insect breeding device; Utilize described video acquisition module to observe the crawler behavior of normal insect, and utilize the second processor from the motion video image of the normal insect of described video acquisition module Real-time Collection, extract the motion video image sequence of normal insect; According to the state parameter of the motion video image sequence of described normal insect and default Insects crawler behavior, set up HMM; According to HMM, obtain normal insect behavior state sequence;
In described insect breeding device, place the insect of radical response, and be allowed to condition at normal life in described insect breeding device; Utilize described video acquisition module to observe the crawler behavior of radical response insect, and utilize the second processor from the motion video image of the radical response insect of described video acquisition module Real-time Collection, extract the motion video image sequence of radical response insect; According to the state parameter of the motion video image sequence of radical response insect and default Insects crawler behavior, set up HMM; According to HMM, obtain radical response insect behavior state sequence.
Described normal insect behavior state sequence and radical response insect behavior state sequence are stored.
In another embodiment of the present invention, the become method of light behavioral trait of research provided by the present invention insect also comprises: utilize network technology or video signal transmission technology, the insect activity video image of described video acquisition module collection is uploaded to remote data center, the video image described video acquisition module being collected at remote data center is analyzed, obtain normal insect behavior state sequence, radical response insect behavior state sequence and insect current behavior status switch to be studied, and insect current behavior status switch is compared with described radical response insect behavior state sequence and normal reaction insect behavior state sequence respectively, obtain comparative result.
The research insect that the embodiment of the present invention provides becomes in the method for light behavioral trait, semiconductor light sources in each observation ward, directly send the light of required wavelength in each observation ward or intensity of illumination, solved in prior art scheme, utilization is filtered artificial light source and is regulated, while obtaining required wavelength or intensity, the problem that precision is lower, has improved the accuracy of result of study.And, be arranged on the second through hole for the video acquisition module of the motion video image that entomologizes, can carry out 360 ° of Real Time Observation to the insect in each observation ward, thereby can be continuously or obtain in real time the behavior of insect under different spectrum, intensity of illumination, when analyzing the behavior of insect under different spectrum and intensity of illumination, reliable data are provided.
Embodiment tri-:
The invention provides another kind the become method of light behavioral trait of the device research insect providing in embodiment mono-be provided, as shown in Figure 4, comprising:
Step 201: intensity of illumination or the wavelength of semiconductor light sources in default each observation ward: the second semiconductor light sources in each observation ward is fixed as to a color, as white, the intensity of illumination of semiconductor light sources in default each observation ward, make intensity of illumination be respectively 0.1lux, 1lux, 10lx, 20lx, 30lx, other term harmonizations.
Step 202: fangle and the water of identical and equivalent are placed respectively in same position in the internal chamber of described inner casing and each observation ward, and regularly supplement.Insect to be studied is placed in the internal chamber of described inner casing, so that described insect to be studied shuttles back and forth to each observation ward by described the first through hole, it is freely movable to be allowed to condition between the internal chamber of described inner casing and each observation ward.
Step 203: open video acquisition module, utilize described video acquisition module to monitor the active situation of insect to be studied in each observation ward, so that described video acquisition module is captured in the video image of the insect activity in each observation ward, and collected video image is uploaded to the second processor in host computer (calculator, panel computer).
Step 204: intensity of illumination or the wavelength of controlling described semiconductor light sources, and the image information that described video acquisition module is collected analyzes, obtain the result of walking quickly and keeping away characteristic and behavioral trait under various intensities of illumination of insect to be studied to different illumination intensity.
The research insect that the embodiment of the present invention provides becomes in the method for light behavioral trait, semiconductor light sources in each observation ward, directly send the light of required intensity of illumination in each observation ward, solved in prior art scheme, utilization is filtered artificial light source and is regulated, while obtaining required wavelength or intensity, the problem that precision is lower, has improved the accuracy of result of study.And, be arranged on the second through hole for the video acquisition module of the motion video image that entomologizes, can carry out 360 ° of Real Time Observation to the insect in each observation ward, thereby can be continuously or obtain in real time the behavior of insect under different spectrum, intensity of illumination, when analyzing the behavior of insect under different spectrum and intensity of illumination, reliable data are provided.
In sum, a kind of become apparatus and method of light behavioral trait of insect of studying provided by the present invention, when overcoming being related between the phototaxis of studying insect in prior art and different spectrum, intensity of illumination, experimental provision and the lower problem of method precision, improve the accuracy of result of study.The visual characteristic and behavioral mechanism, the raising that become light behavioral trait and inquire into insect for accurate research insect improve baiting techniques, simultaneously for solving light baiting techniques in agricultural production and significant with illumination relevant issues.
In this specification, various piece adopts the mode go forward one by one to describe, and each part stresses is the difference with other parts, between various piece identical similar part mutually referring to.
Above-mentioned explanation to the disclosed embodiments, makes professional and technical personnel in the field can realize or use the present invention.To the multiple modification of these embodiment, will be apparent for those skilled in the art, General Principle as defined herein can, in the situation that not departing from the spirit or scope of the present invention, realize in other embodiments.Therefore, the present invention will can not be restricted to embodiment illustrated herein, but will meet the widest scope consistent with principle disclosed herein and features of novelty.
Claims (14)
1. study the become device of light behavioral trait of insect, it is characterized in that, comprising:
Insect breeding device, described insect breeding device is gas permeability device, comprise: mutually nested and light tight inner casing that two ends are concordant and light tight shell, the light tight top cover that covers described inner casing and shell two ends completely and light tight bottom and the annular space that described inner casing, shell, top cover and bottom are formed are divided into the light tight division board of a plurality of observation ward, wherein, between described inner casing lower end and described each observation ward, and between described division board and bottom, have for insect first through hole that shuttles back and forth, between described division board and top cover, there is the second through hole;
Be arranged on wavelength and the adjustable semiconductor light sources of intensity of illumination in each observation ward;
Be arranged on the second through hole for the video acquisition module of the motion video image that entomologizes;
Control wavelength and/or the intensity of illumination of described semiconductor light sources, and the processor that obtains and analyze described video acquisition module information.
2. a kind of become device of light behavioral trait of insect of studying according to claim 1, is characterized in that, described processor comprises:
Be connected with described semiconductor light sources, regulate described semiconductor light sources, make the intensity of illumination first processor consistent with default intensity of illumination of tested point in each observation ward;
Be connected with described video acquisition module, the sequence of video images of extraction activity insect the insect activity video image gathering from described video acquisition module, and according to the sequence of video images of described movable insect and the state parameter of the Insects crawler behavior obtaining in advance set up HMM, and according to HMM, obtain the second processor of insect behavior state sequence.
3. a kind of become device of light behavioral trait of insect of studying according to claim 1, is characterized in that, also comprises:
Be connected with described processor, record the memory of insect behavior state sequence.
4. a kind of become device of light behavioral trait of insect of studying according to claim 3, is characterized in that, described memory comprises:
Record the first memory of radical response insect behavior state sequence and normal reaction insect behavior state sequence;
Record insect current behavior status switch respectively with described first memory in the second memory of comparative result of radical response insect behavior state sequence and normal reaction insect behavior state sequence.
5. a kind of become device of light behavioral trait of insect of studying according to claim 1, is characterized in that, also comprises:
Be connected with described video acquisition module, the insect activity video image of described video acquisition module collection be uploaded to the communication device of remote data center.
6. a kind of become device of light behavioral trait of insect of studying according to claim 1, is characterized in that, described semiconductor light sources comprises:
Be used to plant that luminous energy is provided, the first semiconductor light sources that it can be grown in enclosure space; With,
At least one is for luring the second semiconductor light sources of worm and/or driving.
7. a kind of become device of light behavioral trait of insect of studying according to claim 1, is characterized in that, described insect breeding device also comprises: be arranged at the fluorescence cloth on described side wall of outer shell and described division board sidewall.
8. a kind of become device of light behavioral trait of insect of studying according to claim 1, is characterized in that, described insect breeding device also comprises: be opened in feeding port on described shell and for sealing the shutter of described feeding port.
9. study the become method of light behavioral trait of insect, it is characterized in that, be applied to device described in claim 1, comprising:
Intensity of illumination or the wavelength of semiconductor light sources in default each observation ward;
Insect to be studied is positioned in the internal chamber of described inner casing, so that described insect to be studied is shuttled back and forth to each observation ward by described the first through hole;
Open video acquisition module, so that described video acquisition module is captured in the video image of the insect activity in each observation ward;
Control intensity of illumination or the wavelength of described semiconductor light sources, and the image information that described video acquisition module is collected is analyzed.
10. a kind of become method of light behavioral trait of insect of studying according to claim 9, is characterized in that, intensity of illumination or the wavelength of controlling described semiconductor light sources comprise:
Regulate described semiconductor light sources, make the intensity of illumination of tested point in each observation ward consistent with default intensity of illumination.
11. a kind of become methods of light behavioral trait of insect of studying according to claim 9, is characterized in that, the image information analysis that described video acquisition module is collected comprises:
The sequence of video images of extraction activity insect from the insect activity video image of described video acquisition module Real-time Collection;
According to the state parameter of the sequence of video images of described movable insect and default Insects crawler behavior, set up HMM;
According to HMM, obtain insect current behavior status switch.
12. a kind of become methods of light behavioral trait of insect of studying according to claim 11, is characterized in that, the state parameter of described default Insects crawler behavior calculates according to insect activity cybernetics control number in insect breeding device.
13. a kind of become methods of light behavioral trait of insect of studying according to claim 9, is characterized in that, also comprise:
Pre-stored radical response insect behavior state sequence and normal reaction insect behavior state sequence;
Record insect current behavior status switch respectively with the comparative result of described radical response insect behavior state sequence and normal reaction insect behavior state sequence.
14. a kind of become methods of light behavioral trait of insect of studying according to claim 13, is characterized in that, also comprise: the insect activity video image of described video acquisition module collection is uploaded to remote data center.
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| CN103734084B (en) * | 2013-10-09 | 2016-01-13 | 中国农业科学院蜜蜂研究所 | A kind ofly detect the method affected before allogenic material becomes honeybee to honeybee |
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| CN107810927B (en) * | 2017-11-10 | 2021-07-27 | 华中农业大学 | Method for promoting mating and oviposition of hermetia illucens |
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