CN110150237A - A kind of method of indoor quickly measurement lasioderma serricorne phototaxis - Google Patents
A kind of method of indoor quickly measurement lasioderma serricorne phototaxis Download PDFInfo
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- CN110150237A CN110150237A CN201910532565.7A CN201910532565A CN110150237A CN 110150237 A CN110150237 A CN 110150237A CN 201910532565 A CN201910532565 A CN 201910532565A CN 110150237 A CN110150237 A CN 110150237A
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- larva
- adult
- lasioderma serricorne
- phototaxis
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- 241001177117 Lasioderma serricorne Species 0.000 title claims abstract description 45
- 230000029264 phototaxis Effects 0.000 title claims abstract description 34
- 238000005259 measurement Methods 0.000 title claims abstract description 19
- 238000000034 method Methods 0.000 title claims abstract description 18
- 238000012360 testing method Methods 0.000 claims abstract description 19
- 238000012545 processing Methods 0.000 claims abstract description 9
- 238000013102 re-test Methods 0.000 claims abstract description 4
- 238000010998 test method Methods 0.000 claims description 3
- 230000035945 sensitivity Effects 0.000 abstract description 3
- 235000003642 hunger Nutrition 0.000 abstract 1
- 230000037351 starvation Effects 0.000 abstract 1
- 210000004907 gland Anatomy 0.000 description 22
- 241000238631 Hexapoda Species 0.000 description 12
- 244000309464 bull Species 0.000 description 9
- 238000012546 transfer Methods 0.000 description 7
- 235000013339 cereals Nutrition 0.000 description 4
- 230000002265 prevention Effects 0.000 description 4
- 241000208125 Nicotiana Species 0.000 description 2
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 235000021050 feed intake Nutrition 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 230000000266 injurious effect Effects 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 230000033001 locomotion Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000029305 taxis Effects 0.000 description 2
- 230000007704 transition Effects 0.000 description 2
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000019506 cigar Nutrition 0.000 description 1
- 235000019504 cigarettes Nutrition 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 235000019505 tobacco product Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The invention proposes a kind of methods of indoor quickly measurement lasioderma serricorne phototaxis, its step are as follows: 1) with 24 for one group, the adult of lasioderma serricorne is divided into adult group 1, adult group 2 and adult group 3, the larva of lasioderma serricorne is divided into larva group 1, larva group 2 and larva group 3;2) adult group 1 and larva group 1 processing in hungry 0 day, the processing in hungry 1 day of adult group 2 and larva group 2, adult group 3 and 3 starvation of larva group are handled for 2 days;3) phototaxis test is carried out to adult group 1, adult group 2, adult group 3, larva group 1, larva group 2 and larva group 3 respectively using the LED light source of six kinds of different wave lengths, is averaged after retest 3-5 times;The present invention can be used for studying lasioderma serricorne simultaneously to multi-wavelength's light source sensitivity tests, it is possible to reduce the interference problem of different wave length improves the accuracy of result of study.
Description
Technical field
The present invention relates to a kind of measuring method, especially a kind of method of indoor quickly measurement lasioderma serricorne phototaxis.
Background technique
Lasioderma serricorne is a kind of global stored product insect, and feeding habits are complicated.Cereal, beans, oil are endangered in grain depot
The grains such as material;The tobacco leaf of storage, the tobacco products such as cigarette and cigar are endangered in tobacco leaf warehouse.Usually there is chemistry to its prevention and treatment
Prevention and treatment, cultural control, biological control and physical control etc..Wherein in physical control it is most common be using insect taxis-such as
Phototaxis prevents and treats pest.
Phototaxis refers to the positive negative taxis of biological photic stimuli.At present in production practices comparative maturity utilize elder brother
In the case of worm Phototactic behavior pest control, best with black light lamp and frequency vibration lamp effect, light trap technology has become one kind
Efficient and environmentally friendly physical control means play considerable effect in agriculture and forestry injurious insect monitoring prevention and treatment.Insect Phototactic behavior
It is the important biological characteristics of insect, is a kind of instinct that it adapts to and formed for a long time to environment.Due to the insect habitats ring of light
Border is varied, and Phototactic behavior also has very big difference, thus, the Phototactic behavior of insect is observed, determines that insect is sensitive single
Coloured light wavelength is beneficial to application of the light trap technology in agriculture and forestry injurious insect prevention and treatment.
Have longer history to the become research of light of insect both at home and abroad, and there is preferable practice value, such as: Chang Cai
It uses the fluorescent lamp of different wave length as light source, improves observation insect to the taxis of different light sources, and then determine that insect is more sensitive
Optical band.However the device of these Phototactic behaviors is relatively simple, the light source once surveyed is less, and exists between light source mutual
The phenomenon that interference, influences the accuracy of test.
Summary of the invention
The present invention can use in view of the above-mentioned problems, to propose a kind of method of indoor quickly measurement lasioderma serricorne phototaxis
Lasioderma serricorne is studied to multi-wavelength's light source sensitivity tests in simultaneously, it is possible to reduce the interference problem of different wave length improves research
As a result accuracy.
Specific technical solution is as follows:
A kind of method of indoor quickly measurement lasioderma serricorne phototaxis, its step are as follows:
1) adult of lasioderma serricorne is divided into adult group 1, adult group 2 and adult group 3, by lasioderma serricorne for one group with 24
Larva is divided into larva group 1, larva group 2 and larva group 3;
2) adult group 1 and larva group 1 processing in hungry 0 day, the processing in hungry 1 day of adult group 2 and larva group 2, adult group 3 and children
It handles within worm group 3 hungry 2 days;
3) using the LED light source of six kinds of different wave lengths respectively to adult group 1, adult group 2, adult group 3, larva group 1, larva
Group 2 and larva group 3 carry out phototaxis test, are averaged after retest 3-5 times.
Further, step 3) carries out phototaxis test using rapid determination device.
Further, rapid determination device includes sample placed cavity, and uniformly distributed six transfers are logical on the side wall of sample placed cavity
Road is socketed a receiving cavity on each transfering channel respectively, a LED light source is correspondingly arranged on the outside of each receiving cavity, each
A light source cover is covered respectively on LED light source transfering channel corresponding with its, prevents the light source of six transfering channels from interfering with each other.
Further, the test method of rapid determination device are as follows: place one group of 24 lasioderma serricorne in sample placed cavity, often
1h, 2h, 4h and for 24 hours respectively record six receiving cavities in lasioderma serricorne quantity data;It repeats 3-5 times.
Further, the wavelength of six kinds of LED light sources is respectively 395-400nm, 450-480nm, 520-525nm, 540-
565nm, 620-625nm and 6500k.
As a further refinement of the present invention, different type can be placed in six receiving cavities of rapid determination device
Food, to measure food to the allure of lasioderma serricorne.
As a further refinement of the present invention, additionally providing a kind of device of quickly measurement lasioderma serricorne phototaxis.
Further, which includes support base, and support base is equipped with test device;
Test device includes the sample placed cavity of open-topped cylindrical structure, is evenly distributed with six on the side wall of sample placed cavity
The bottom of sample placed cavity is arranged in a transfering channel, transfering channel, and being arranged for being interference fitted on the outboard end of transfering channel is solid
Determine a receiving cavity, a light source fixing seat is correspondingly provided on the outside of each receiving cavity, each light source fixing seat is equipped with one
LED bulb.
Further, support base is equipped with a gland component;
Gland component includes gland, corresponding with six transfering channels on gland to set that there are six light source block covers, when gland lid
When being located in support base, each light source block cover is located at the outside of corresponding light source fixing seat and receiving cavity respectively.
Further, an observation opening is respectively equipped on gland on the position of corresponding six receiving cavities, is observed in opening
Hingedly fix an observation window.
Further, the position of counter sample placed cavity is equipped with the opening that feeds intake on gland, and feed intake the upper clamping that is open
Fix a cover board, the bottom of cover board is equipped with corking, the opening of the insertion sample placed cavity of corking transition fit, in corking with
What cover board was connected is equipped with multiple air holes.
Further, light source fixing seat includes fulcrum bearing, is rotatably equipped with fixed plate, the inside of fixed plate in fulcrum bearing
Wall is equipped with lamp cap, and LED bulb is fixed on lamp cap;
Fulcrum bearing is equipped with the first magnet, and the first magnet is arranged in the inside of fixed plate, in fixed plate with the first magnet pair
That answers is equipped with the second magnet, and the polarity of the second magnet and the opposite face of the first magnet rotates on the contrary, working as fixed plate to being vertically arranged
When, the second magnet and the first magnet mutually adsorb fixation.
Further, channel movable component is equipped in sample placed cavity;Channel movable component includes being rotatably arranged on
The bottom plate of sample placed cavity bottom, vertical on bottom plate to be equipped with a circle outer baffle, the inner sidewall of outer baffle and sample placed cavity pastes
Setting is closed, it is corresponding with six transfering channels on outer baffle to set that there are six transfer openings;
It is vertical at the center of bottom be equipped with a bull stick, it is upwardly extended at the top of rotation and clearance fit passes through lid
Plate.
Further, the non-return ring of interior big outer small truncated cone-shaped structure is equipped in transfering channel, the inner end of non-return ring is viscous
It connects and is fixed in transfering channel, non-return passage is equipped in non-return ring, non-return passage is interior big outer small truncated cone-shaped structure.
The application method of the device of the quick measurement lasioderma serricorne phototaxis are as follows:
(1) gland is opened, rotation bull stick makes outer baffle block transfering channel, receiving cavity is fixed on to the outside of transfering channel
End, and sample placed cavity is fixed in support base;
(2) fixed plate is stirred outward, lamp cap is set upward, is separately connected different wave length in each light source fixing seat
After LED bulb, then fixed plate is inwardly stirred until the first magnet and the second magnet mutually adsorb fixation;
(3) after opening cover board, gland pressure is set and is fixed in support base, is located at six light source block covers respectively accordingly
Light source fixing seat and receiving cavity it is upper;
(4) lasioderma serricorne is put into sample placed cavity by the opening that feeds intake, covers cover board and passes through bull stick rotatably
Cover board;
(5) six LED bulbs are opened, rotation bull stick is correspondingly arranged transfer opening with transfering channel, and transfering channel is in
Opening state, lasioderma serricorne pass through different transfering channels and enter that different receiving cavities is total, can by the movement of lasioderma serricorne into
Enter the quantity of different receiving cavities to detect its phototaxis.
The invention has the benefit that
(1) present invention can do multiple processing simultaneously, and the error of many experiments will be carried out by reducing different disposal;
(2) what the present invention can carry out Multiple factors lures experiment, and flexibility is higher;
(3) present invention can be used for studying lasioderma serricorne simultaneously to multi-wavelength's light source sensitivity tests;
(4) setting of light source block cover, it is possible to reduce the interference problem of different wave length improves the accuracy of result of study;
(5) setting of observation window can observe the mobile trend of lasioderma serricorne at any time during the test;
(6) setting of non-return ring makes lasioderma serricorne by that can not return easily after non-return passage, further improves test
Accuracy.
Detailed description of the invention
The structure chart of the rapid determination device of Fig. 1 embodiment of the present invention one;
Fig. 2 is the cross-sectional view of the device of the quick measurement lasioderma serricorne phototaxis of the embodiment of the present invention two;
Fig. 3 is support base top view;
Fig. 4 is gland component top view;
Fig. 5 is gland component bottom view;
Partial enlarged view in Fig. 6 Fig. 3;
Fig. 7 non-return ring partial enlarged view.
Appended drawing reference:
Support base 1, sample placed cavity 2, transfering channel 3, receiving cavity 4, light source fixing seat 5, LED bulb 6, gland component
7;
Gland 101, light source block cover 102, observation window 103, cover board 104, corking 105;
Fulcrum bearing 201, fixed plate 202, lamp cap 203, the first magnet 204, the second magnet 205;Bottom plate 301, outer baffle
302, transfer opening 303, bull stick 304;
Non-return ring 401, non-return passage 402.
Specific embodiment
It is clear to be more clear technical solution of the present invention, below the present invention is described further, any pair of sheet
The technical characteristic of inventive technique scheme carries out the scheme that equivalencing is obtained with conventional reasoning and each falls within the scope of the present invention.
Embodiment one, embodiment two, the raw material in embodiment three and equipment are Zhejiang Province's emphasis development project
The provincial grain depot technology of fringe in (2017C02G2100800), state key research and development plan (2018YFD0401403), Zhejiang
Joint development project (warehousing department 2018-001) is subsidized.
Embodiment one
A kind of method of indoor quickly measurement lasioderma serricorne phototaxis, its step are as follows:
1) adult of lasioderma serricorne is divided into adult group 1, adult group 2 and adult group 3, by lasioderma serricorne for one group with 24
Larva is divided into larva group 1, larva group 2 and larva group 3;
2) adult group 1 and larva group 1 processing in hungry 0 day, the processing in hungry 1 day of adult group 2 and larva group 2, adult group 3 and children
It handles within worm group 3 hungry 2 days;
3) using the LED light source of six kinds of different wave lengths respectively to adult group 1, adult group 2, adult group 3, larva group 1, larva
Group 2 and larva group 3 carry out phototaxis test, are averaged after retest 3-5 times;The wavelength of six kinds of LED light sources is respectively
395-400nm, 450-480nm, 520-525nm, 540-565nm, 620-625nm and 6500k.
Step 3) carries out phototaxis test using rapid determination device.
As shown in Figure 1, rapid determination device includes sample placed cavity 2, uniformly distributed six transfers on the side wall of sample placed cavity
Channel 3 is socketed a receiving cavity 4 on each transfering channel respectively, a LED bulb 6 is correspondingly arranged on the outside of each receiving cavity,
A light source cover is covered in each LED bulb respectively.
The test method of rapid determination device are as follows: place one group of 24 lasioderma serricorne in sample placed cavity, every 1h, 2h, 4h
Record the data of the quantity of the lasioderma serricorne in six receiving cavities respectively for 24 hours, test result see the table below.
By above-mentioned test result, it can be concluded that, adult is most sensitive to the light source reaction of 395-400nm wavelength, and larva is right
The light source reaction of 620-625nm wavelength is most sensitive.
Embodiment two
As shown in Fig. 2-Fig. 7, a kind of device of indoor quickly measurement lasioderma serricorne phototaxis, including support base 1, support bottom
Seat is equipped with test device;
Test device includes the sample placed cavity 2 of open-topped cylindrical structure, is evenly distributed on the side wall of sample placed cavity
The bottom of sample placed cavity is arranged in six transfering channels 3, transfering channel, and what is be interference fitted on the outboard end of transfering channel is arranged
A receiving cavity 4 is fixed, a light source fixing seat 5 is correspondingly provided on the outside of each receiving cavity, each light source fixing seat is equipped with
One LED bulb 6.
Further, support base is equipped with a gland component 7;
Gland component includes gland 101, corresponding with six transfering channels on gland to set there are six light source block cover 102, when
When gland lid is located in support base, each light source block cover is located at the outside of corresponding light source fixing seat and receiving cavity respectively.
Further, an observation opening is respectively equipped on gland on the position of corresponding six receiving cavities, is observed in opening
Hingedly fix an observation window 103.
Further, the position of counter sample placed cavity is equipped with the opening that feeds intake on gland, and feed intake the upper clamping that is open
A cover board 104 is fixed, the bottom of cover board is equipped with corking 105, the opening of the insertion sample placed cavity of corking transition fit, plug
Cover be connected with cover board be equipped with multiple air holes.
Further, light source fixing seat includes fulcrum bearing 201, is rotatably equipped with fixed plate 202, fixed plate in fulcrum bearing
Inner sidewall be equipped with lamp cap 203, LED bulb is fixed on lamp cap;
Fulcrum bearing is equipped with the first magnet 204, and the first magnet is arranged in the inside of fixed plate, in fixed plate with the first magnet
Corresponding to be equipped with the second magnet 205, the polarity of the second magnet and the opposite face of the first magnet rotates on the contrary, working as fixed plate to vertical
When setting, the second magnet and the first magnet mutually adsorb fixation.
Further, channel movable component is equipped in sample placed cavity;Channel movable component includes being rotatably arranged on
The bottom plate 301 of sample placed cavity bottom, vertical on bottom plate to be equipped with a circle outer baffle 302, outer baffle is interior with sample placed cavity
Side wall fitting setting, it is corresponding with six transfering channels on outer baffle to set that there are six transfer openings 303;
It is vertical at the center of bottom be equipped with a bull stick 304, it is upwardly extended at the top of rotation and clearance fit passes through
Cover board.
Further, the non-return ring 401 of interior big outer small truncated cone-shaped structure, the inner end of non-return ring are equipped in transfering channel
It is adhesively fixed in transfering channel, non-return passage 402 is equipped in non-return ring, non-return passage is interior big outer small truncated cone-shaped structure.
The application method of the device of lasioderma serricorne phototaxis is quickly measured in the present embodiment room are as follows:
(1) gland is opened, rotation bull stick makes outer baffle block transfering channel, receiving cavity is fixed on to the outside of transfering channel
End, and sample placed cavity is fixed in support base;
(2) fixed plate is stirred outward, lamp cap is set upward, is separately connected different wave length in each light source fixing seat
After LED bulb, then fixed plate is inwardly stirred until the first magnet and the second magnet mutually adsorb fixation;
(3) after opening cover board, gland pressure is set and is fixed in support base, is located at six light source block covers respectively accordingly
Light source fixing seat and receiving cavity it is upper;
(4) lasioderma serricorne is put into sample placed cavity by the opening that feeds intake, covers cover board and passes through bull stick rotatably
Cover board;
(5) six LED bulbs are opened, rotation bull stick is correspondingly arranged transfer opening with transfering channel, and transfering channel is in
Opening state, lasioderma serricorne pass through different transfering channels and enter that different receiving cavities is total, can by the movement of lasioderma serricorne into
Enter the quantity of different receiving cavities to detect its phototaxis.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
In the technical scope disclosed by the present invention, any changes or substitutions that can be easily thought of by anyone skilled in the art,
It should be covered by the scope of protection of the present invention.
Claims (6)
1. a kind of method of indoor quickly measurement lasioderma serricorne phototaxis, it is characterized in that, its step are as follows:
1) adult of lasioderma serricorne is divided into adult group 1, adult group 2 and adult group 3, by the larva of lasioderma serricorne for one group with 24
It is divided into larva group 1, larva group 2 and larva group 3;
2) adult group 1 and larva group 1 processing in hungry 0 day, the processing in hungry 1 day of adult group 2 and larva group 2, adult group 3 and larva group
3 are handled for hungry 2 days;
3) using the LED light source of six kinds of different wave lengths respectively to adult group 1, adult group 2, adult group 3, larva group 1, larva group 2
Phototaxis test is carried out with larva group 3, is averaged after retest 3-5 times.
2. a kind of method of indoor quickly measurement lasioderma serricorne phototaxis as described in claim 1, it is characterized in that, step 3) uses
Rapid determination device carries out phototaxis test.
3. a kind of method of indoor quickly measurement lasioderma serricorne phototaxis as claimed in claim 2, it is characterized in that, quick measurement dress
It sets including sample placed cavity, uniformly distributed six transfering channels on the side wall of sample placed cavity, is socketed one respectively on each transfering channel
A receiving cavity is correspondingly arranged a LED light source on the outside of each receiving cavity.
4. a kind of method of indoor quickly measurement lasioderma serricorne phototaxis as claimed in claim 3, it is characterized in that, quick measurement dress
The test method set are as follows: one group of 24 lasioderma serricorne is placed in sample placed cavity, every 1h, 2h, 4h and is recorded one time six respectively for 24 hours
The data of the quantity of lasioderma serricorne in a receiving cavity repeat 3-5 times.
5. a kind of method of indoor quickly measurement lasioderma serricorne phototaxis as described in claim 1,2,3 or 4, it is characterized in that, six
The wavelength of kind of LED light source be respectively 395-400nm, 450-480nm, 520-525nm, 540-565nm, 620-625nm and
6500k。
6. a kind of method of indoor quickly measurement lasioderma serricorne phototaxis as claimed in claim 5, it is characterized in that, each LED light
A light source cover is covered respectively on the transfering channel corresponding with its of source.
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CN201910532565.7A CN110150237A (en) | 2019-06-19 | 2019-06-19 | A kind of method of indoor quickly measurement lasioderma serricorne phototaxis |
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CN201910532565.7A CN110150237A (en) | 2019-06-19 | 2019-06-19 | A kind of method of indoor quickly measurement lasioderma serricorne phototaxis |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN111616122A (en) * | 2020-07-08 | 2020-09-04 | 陕西新金叶烟草科技有限公司 | Tobacco beetle light trap and trapping method |
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CN102696553A (en) * | 2012-04-27 | 2012-10-03 | 河北省农林科学院植物保护研究所 | Insect wavelength selector and application thereof |
CN102960314A (en) * | 2012-12-17 | 2013-03-13 | 杭州汉徽光电科技有限公司 | Device and method for studying phototactic behavior characteristics of inserts |
CN206150268U (en) * | 2016-10-31 | 2017-05-10 | 华南农业大学 | Modular many light sources insect becomes to being bare action reaction unit |
CN107810935A (en) * | 2017-12-11 | 2018-03-20 | 中国计量大学 | A kind of multispectral trap lamp experimental provision and method |
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2019
- 2019-06-19 CN CN201910532565.7A patent/CN110150237A/en active Pending
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JP2009039128A (en) * | 2008-09-22 | 2009-02-26 | N Bii T Kk | Method for manufacturing light source for insect control |
CN102578049A (en) * | 2012-02-29 | 2012-07-18 | 成都鼎明光电科技有限公司 | Insect phototaxis testing device |
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