CN102919133A - Technical method for promoting tissue culture rooting of alpine rose amethyst - Google Patents
Technical method for promoting tissue culture rooting of alpine rose amethyst Download PDFInfo
- Publication number
- CN102919133A CN102919133A CN2012105066792A CN201210506679A CN102919133A CN 102919133 A CN102919133 A CN 102919133A CN 2012105066792 A CN2012105066792 A CN 2012105066792A CN 201210506679 A CN201210506679 A CN 201210506679A CN 102919133 A CN102919133 A CN 102919133A
- Authority
- CN
- China
- Prior art keywords
- amethyst
- days
- culture
- technical method
- alpine rose
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention discloses a technical method for promoting the tissue culture rooting of alpine rose amethyst. The method comprises the following steps of: cutting off robust sterile cluster buds by scissors on a sterile ultra-clean work table, and inoculating into the following rooting culture medium, namely a 1/2 WPM minimal medium, adding 10mg/L of added NAA (l-naphthylacetic acid), 10g/L of cane sugar, 0.3g/L of activated carbon and 6g/L of agar powder. The method comprises the following steps of: firstly, carrying out 3 days of dark culture, carrying out 7 days of light culture, taking out bud seedlings on the sterile ultra-clean work table, and transferring into the hormone-free 1/2 WPM minimal medium to continuously carry out 30-40 days of light culture. After the method is used, the rooting percentage of the tissue culture seedling can be improved by more than 30%, the rooting percentage of the amethyst tissue culture seedling can be greatly improved, the tissue culture seedling root system treated by the method has thicker and more secondary branches and can be more easily transplanted to be survived.
Description
One, technical field
The invention belongs to field of plant growing technology, especially woody ornamentais tissue culture and rapid propagation method field is specifically related to a kind of technical method that promotes that the training of alpine rose amethyst group is taken root.
Two, background technology
Alpine rose is the general name of evergreen broad-leaved kind and filial generation thereof in the Ericaceae Rhododendron, often grows on the above high mountain of height above sea level 1000m.Amethyst (Rhododendron hybrids ' Roseum Elegans ') is one of more welcome alpine rose in the market, evergreen shrubs or dungarunga, 3 meters of Gao Keda, florescence 4-5 month, flower is the lilac that shows slightly rose, funnel-form, and single flower diameter reaches 6cm, petal is with brown floral designs, total shape umbellate form inflorescence diameter can reach 15-30cm, and the large color depth of leaf is special cold-resistant and heat-resisting, do not fall leaves under-26 ℃, premunition is strong.Amethyst is very large because of its inflorescence, pattern is bright-coloured, the liking of the evergreen all the year round people of enjoying of blade, be not only the annual flower grass of domestic top grade, also be that high-grade in the world afforestation beautifies material, will become the rising star that China Top-grade-landscaping seeds are used, have the wide prospect of marketing.
At present, the alpine rose of selling on the domestic market is from external import substantially entirely, and to thousands of units, price is always high up to hundreds of for every basin price, and seedling market is well sold and in short supply.And find in the course of cultivation the problems such as the alpine rose cuttage survival rate is low, the separation of seminal propagation proterties, therefore the domestic research that has had researcher to be engaged in the alpine rose tissue culture technique, the kind of successfully setting up the tissue culture quick breeding system has delavay rhododendron, Rhododendron fortuneilindl． and some external kinds (Lee ' s Dark Purple, Rocket, Jean de Marle Montage, Nova Zembla, Cosmopolitan etc.), but does not appear in the newspapers yet in the research that the amethyst tissue is cultivated the technical elements of particularly taking root.
Three, summary of the invention
The object of the present invention is to provide a kind of technical method that promotes that the training of alpine rose amethyst group is taken root.
For realizing purpose of the present invention, the invention provides following technical scheme:
1, a kind of method that promotes alpine rose group training seedling rooting, its content comprises: on aseptic superclean bench, the aseptic Multiple Buds of robust growth is cut with scissors, be inoculated in the following root media: the 1/2WPM minimal medium, add NAA10mg/L, sucrose 10g/L, active carbon 0.3g/L, agar powder 6g/L.Carry out first 3 days dark cultivation, after then light is cultivated and finished in 7 days, on aseptic superclean bench, the bud seedling is taken out, be transferred in the 1/2WPM minimal medium that does not contain hormone, proceed 30-40 days light and cultivate.
2, in the present invention: the WPM medium is the document with reference to Lloyd G and McCown B (1980), 1/2WPM is that macroelement and micro-consumption are half of element consumption in the former document, and other elements (comprising organic element and molysite) are all constant.List of references: LloydG and McCown B.Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoottip culture[J] .Combined Proceedings-International Plant Propagators ' Society, 1980,30:421-427
3, in the present invention: NAA is methyl α-naphthyl acetate (1-naphthylacetic acid).
4, in the present invention: employing be the cone-shaped glass bottle of 100ml as culture vessel, the medium of packing is 40-50ml in every bottle, after medium added hormone and transfers to pH value 53-5.8, packing was at 121 ℃ of lower autoclaving 20min.
5, in the present invention: the light condition of culture is that day temperature is 25 ± 1 ℃, and nocturnal temperature is 20 ± 1 ℃, and intensity of illumination is 50 μ molm
-2S
-1, illumination every day 12h.Dark condition of culture is that day temperature is 25 ± 1 ℃, and nocturnal temperature is 20 ± 1 ℃, unglazed photograph.
The present invention preferably sums up the technical parameter of above-mentioned the best through following test:
(1), use orthogonal design, from different hormone kinds, hormone concentration, turn the situation that fate before blank cultivation the, 4 aspects of sugared consumption are inquired into amethyst group training seedling rooting, use L
9(3)
4Orthogonal table is established 9 processing altogether, not add hormone and 30g/L sugar as contrast, experimental design and result such as following table 1:
Table 1 alpine rose amethyst group is trained the orthogonal experiments analytical table of taking root
According to quadrature analysis, to select NAA, concentration when hormone kind be 10mg/L, turn before blank the cultivation fate is 10d and sugared consumption when being 10g/L, and rooting efficiency will be best.
(2), on the basis of test (1), optimum test treatment combination (NAA 10mg/L in the medium, turn fate is that 10d and sugared consumption are 10g/L before blank the cultivation) is compared test with orthogonal experiment 4.The result shows that the rooting rate of optimum test treatment combination and orthogonal experiment 4 is respectively 56.8%, 42.1%, and the two exists significant difference, and the group of optimum test treatment combination training shoot root system is more sturdy, root system secondary branch is more.
Embodiment
For further understanding summary of the invention of the present invention and effect, hereby exemplify following examples, if employed experimental technique is conventional method without specified otherwise among the embodiment:
1, the preparation of aseptic Multiple Buds: Rooting began front 60 days, choosing healthy and strong amethyst plant stem with bud without damage by disease and insect is explant, carrying out cleaning, sterilization, the bud of explant sprouts and inducing clumping bud, concrete steps list of references: Liu Xiaoqing, Su Jiale, Xiang Liping, etc. the foundation [J] of alpine rose stem Duan Zupei cultivation and optimization system. Yangzhou University's journal (agricultural and life science version), 2007,28 (3): 91-94.
2, the preparation of medium: with reference to element consumption in the document of Lloyd G and McCown B in 1980, respectively macroelement and micro-consumption are reduced by half, organic element and molysite consumption are constant, are the 1/2WPM minimal medium.Root media is the NAA that has added 10mg/L on the basis of minimal medium.Minimal medium and root media all are to add 10g/L sucrose, 03g/L active carbon, 6g/L agar powder, and after adjust pH was 53-5.8, packing was at 121 ℃ of lower autoclaving 20min.
3, root induction: choose healthy and strong aseptic Multiple Buds, on aseptic superclean bench, Multiple Buds is cut with scissors, be inoculated in the root media, carry out first 3 days dark cultivation, then carrying out light cultivated 7 days, light on aseptic superclean bench, takes out the bud seedling after cultivating and finishing, directly be transferred in the 1/2WPM minimal medium that does not contain hormone, proceed 30-40 days light and cultivate.
4, domestication and transplant: the tissue-culture container seedling that will take root, transfer to that sunny side is indoor to carry out natural daylight docilityization 5-7 days, and then the sealed membrane of tissue culture bottle removed, and in every bottle the distilled water of interpolation 10ml, carry out nonsterile conditions and tamed 2-3 days.Then, to organize training seedling from bottle, taking out gently with tweezers, the medium that carefully root is sticked in clear water washes, and with the potassium permanganate root immersion of 0.1% carbendazim or 0.3%-0.5% 5 minutes with disinfection, then be transplanted to the cultivation matrix (peat: perlite=4: 1) that carried out disinfection with 5% potassium permanganate in 2 days in advance, place in the greenhouse without the light of heating, shade the domestication 3 weeks, during the irregular 1/2WPM of spraying nutrient solution to guarantee enough nutrition and humidity.3 Zhou Houke carry out conventional field maintenance management.
The above; only be the better embodiment of the present invention; not to concrete restriction of the present invention; but protection scope of the present invention is not limited to this; anyly be familiar with those skilled in the art in the technical scope that the present invention discloses; the variation that can expect easily or replacement all should be encompassed within protection scope of the present invention.
Claims (5)
1. one kind promotes alpine rose amethyst group to train the technical method of taking root, it is characterized in that: on aseptic superclean bench, the aseptic Multiple Buds of robust growth is cut with scissors, be inoculated in the following root media: the 1/2WPM minimal medium, add NAA10mg/L, sucrose 10g/L, active carbon 0.3g/L, agar powder 6g/L.Carry out first 3 days dark cultivation, after then light is cultivated and finished in 7 days, on aseptic superclean bench, the bud seedling is taken out, be transferred in the 1/2WPM minimal medium that does not contain hormone, proceed 30-40 days light and cultivate.
2. promotion alpine rose amethyst group according to claim 1 is trained the technical method of taking root, it is characterized in that: the WPM medium is the document with reference to Lloyd G and McCown B (1980), 1/2WPM is macroelement and micro-consumption half of element consumption in former document, and other elements (comprising organic element and molysite) are all constant.
3. promotion alpine rose amethyst group according to claim 1 is trained the technical method of taking root, and it is characterized in that: NAA is methyl α-naphthyl acetate (l-naphthylacetic acid).
4. promotion alpine rose amethyst group according to claim 1 is trained the technical method of taking root, it is characterized in that: employing be that the cone-shaped glass bottle of 100ml is as culture vessel, the medium of packing is 40-50ml in every bottle, after medium adds hormone and transfers to pH value 5.3-5.8, packing is at 121 ℃ of lower autoclaving 20min.
5. promotion alpine rose amethyst group according to claim 1 is trained the technical method of taking root, and it is characterized in that: the light condition of culture is that day temperature is 25 ± 1 ℃, and nocturnal temperature is 20+1 ℃, and intensity of illumination is 50 μ molm
-2S
-1, illumination every day 12h.Dark condition of culture is that day temperature is 25 ± 1 ℃, and nocturnal temperature is 20 ± 1 ℃, unglazed photograph.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012105066792A CN102919133A (en) | 2012-12-03 | 2012-12-03 | Technical method for promoting tissue culture rooting of alpine rose amethyst |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012105066792A CN102919133A (en) | 2012-12-03 | 2012-12-03 | Technical method for promoting tissue culture rooting of alpine rose amethyst |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102919133A true CN102919133A (en) | 2013-02-13 |
Family
ID=47634184
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012105066792A Pending CN102919133A (en) | 2012-12-03 | 2012-12-03 | Technical method for promoting tissue culture rooting of alpine rose amethyst |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102919133A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103636492A (en) * | 2013-11-14 | 2014-03-19 | 石家庄市农林科学研究院 | Rhododendron hybrides cosmopolitan tissue culture rapid propagation method |
| CN103947551A (en) * | 2014-04-22 | 2014-07-30 | 浙江大学 | Method for promoting germination and sprouting of folium rhododendri daurici seeds |
| CN104521741A (en) * | 2014-12-23 | 2015-04-22 | 江苏省农业科学院 | Method for cultivating aromatic alpine rhododendron |
| CN104885932A (en) * | 2014-12-11 | 2015-09-09 | 华南农业大学 | Tissue culture and rapid propagation method for rhododendron moulmainense |
| CN106358898A (en) * | 2016-08-25 | 2017-02-01 | 平顶山学院 | Cutting cultivation method for improving the rooting rate of azalea |
| CN109349109A (en) * | 2018-11-23 | 2019-02-19 | 云南省农业科学院花卉研究所 | Shaping methods in the bottle of alpine rose young beauty |
| CN113767848A (en) * | 2021-08-25 | 2021-12-10 | 山东省农业科学院 | Efficient rooting method for rhododendron lapponicum tissue culture seedlings |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1630711A1 (en) * | 1989-03-06 | 1991-02-28 | Латвийский Государственный Университет Им.П.Стучки | Method of nutrient substrate preparation for plant tissues of rhododendrons and azaleas cultivation |
| JPH06189643A (en) * | 1992-12-25 | 1994-07-12 | New Oji Paper Co Ltd | Large-scale multiplication of rhododendron |
| JPH06189646A (en) * | 1992-12-25 | 1994-07-12 | New Oji Paper Co Ltd | Method for promoting rooting of culture shoot in plant of genus rhododendron |
| WO1998038294A1 (en) * | 1997-02-27 | 1998-09-03 | Union Camp Corporation | Growth-enhanced transgenic plants |
| CN1568670A (en) * | 2004-04-26 | 2005-01-26 | 上海市闵行区农业科学研究所 | Tissue culture and tachytelic propagating method for alpine rhododendron |
| CN1732759A (en) * | 2005-08-17 | 2006-02-15 | 中国林业科学研究院林业研究所 | Tissue culturing, rapid propagating and transplanting method of Rhododendron mucronulatum Turcz. |
| CN101044840A (en) * | 2006-03-31 | 2007-10-03 | 贵州科学院 | Method for fast breeding western azalea and culture medium therefor |
| CN101292628A (en) * | 2008-06-11 | 2008-10-29 | 国家***泡桐研究开发中心 | Cultivation method for north America rhododendron |
-
2012
- 2012-12-03 CN CN2012105066792A patent/CN102919133A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1630711A1 (en) * | 1989-03-06 | 1991-02-28 | Латвийский Государственный Университет Им.П.Стучки | Method of nutrient substrate preparation for plant tissues of rhododendrons and azaleas cultivation |
| JPH06189643A (en) * | 1992-12-25 | 1994-07-12 | New Oji Paper Co Ltd | Large-scale multiplication of rhododendron |
| JPH06189646A (en) * | 1992-12-25 | 1994-07-12 | New Oji Paper Co Ltd | Method for promoting rooting of culture shoot in plant of genus rhododendron |
| WO1998038294A1 (en) * | 1997-02-27 | 1998-09-03 | Union Camp Corporation | Growth-enhanced transgenic plants |
| CN1568670A (en) * | 2004-04-26 | 2005-01-26 | 上海市闵行区农业科学研究所 | Tissue culture and tachytelic propagating method for alpine rhododendron |
| CN1732759A (en) * | 2005-08-17 | 2006-02-15 | 中国林业科学研究院林业研究所 | Tissue culturing, rapid propagating and transplanting method of Rhododendron mucronulatum Turcz. |
| CN101044840A (en) * | 2006-03-31 | 2007-10-03 | 贵州科学院 | Method for fast breeding western azalea and culture medium therefor |
| CN101292628A (en) * | 2008-06-11 | 2008-10-29 | 国家***泡桐研究开发中心 | Cultivation method for north America rhododendron |
Non-Patent Citations (2)
| Title |
|---|
| 刘晓青等: "高山杜鹃茎段组织培养和优化体系的建立", 《扬州大学学报（农业与生命科学版）》 * |
| 苏家乐等: "高山杜鹃新品种富丽金陵的组培快繁技术", 《江苏农业科学》 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103636492A (en) * | 2013-11-14 | 2014-03-19 | 石家庄市农林科学研究院 | Rhododendron hybrides cosmopolitan tissue culture rapid propagation method |
| CN103636492B (en) * | 2013-11-14 | 2015-07-08 | 石家庄市农林科学研究院 | Rhododendron hybrides cosmopolitan tissue culture rapid propagation method |
| CN103947551A (en) * | 2014-04-22 | 2014-07-30 | 浙江大学 | Method for promoting germination and sprouting of folium rhododendri daurici seeds |
| CN103947551B (en) * | 2014-04-22 | 2016-02-03 | 浙江大学 | A kind of method promoting spring cuckoo seed germination seedling |
| CN104885932A (en) * | 2014-12-11 | 2015-09-09 | 华南农业大学 | Tissue culture and rapid propagation method for rhododendron moulmainense |
| CN104521741A (en) * | 2014-12-23 | 2015-04-22 | 江苏省农业科学院 | Method for cultivating aromatic alpine rhododendron |
| CN106358898A (en) * | 2016-08-25 | 2017-02-01 | 平顶山学院 | Cutting cultivation method for improving the rooting rate of azalea |
| CN109349109A (en) * | 2018-11-23 | 2019-02-19 | 云南省农业科学院花卉研究所 | Shaping methods in the bottle of alpine rose young beauty |
| CN113767848A (en) * | 2021-08-25 | 2021-12-10 | 山东省农业科学院 | Efficient rooting method for rhododendron lapponicum tissue culture seedlings |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105475130B (en) | A kind of red cone isolated culture plant strain regeneration method | |
| CN102919133A (en) | Technical method for promoting tissue culture rooting of alpine rose amethyst | |
| CN103314855A (en) | Bletilla seed tissue culture propagation method | |
| CN103704130B (en) | A kind of method of Chunlan and the nursery of hybrid cymbidium crossbreed | |
| Zeng et al. | Asymbiotic seed germination, induction of calli and protocorm-like bodies, and in vitro seedling development of the rare and endangered Nothodoritis zhejiangensis Chinese orchid | |
| CN102845313A (en) | Method for quickly in-vitro actinidia kolomikta propagating | |
| CN103460971B (en) | Method for improving transplanting survival rate of trichosanthes kirilowii tissue culture seedlings | |
| CN106718877B (en) | A kind of flourishing torch Radix Curcumae high quality seedling rapid propagation method | |
| CN104041412A (en) | Rapid propagation method for tissue culture of Guizhou hemiboea cavaleriei | |
| Riva et al. | In vitro regeneration and rapid multiplication of Dendrobium bensoniae, an indigenous ornamental orchid. | |
| CN103404438B (en) | Acer palmatum seed tissue culture method | |
| CN109349105A (en) | A kind of iris tissue-cultured seedling mating system | |
| CN106538382B (en) | Method for establishing efficient eremochloa ophiuroides regeneration system by taking young ears as explants | |
| CN106258960B (en) | A kind of orchid seed sprouting quick-breeding method | |
| CN103583360B (en) | A kind of directional induction improves the method for Abelia biflora nursery stock salt resistance | |
| CN103404437B (en) | Novel method for tissue culture rapid propagation of acer paimatum | |
| CN102893872A (en) | Tissue culture method for domesticated seedlings of iris pallida | |
| CN106954552A (en) | A kind of tissue culture propagation method of cinnamomum camphora stem section | |
| CN104488723A (en) | Tissue-culture and rapid-propagation method of epimedium koreanum nakai | |
| CN104304238A (en) | Embedding drying ultralow temperature preservation method of vitis heyneana stem tip | |
| CN103651141A (en) | Factory-like rapid propagation method for test-tube seedlings of dendranthema morifolium | |
| CN101766123B (en) | Method for rapid propagation of zephyr lily | |
| CN102907325B (en) | Method for utilizing culture technology to produce Solomon turmeric and red tulip seedlings | |
| CN109247235B (en) | Rapid breeding and seedling raising method for cymbidium faberi Rolfe | |
| CN103621405A (en) | Artificial corychophramus violaceua seed making method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130213 |