CN103947551B - A kind of method promoting spring cuckoo seed germination seedling - Google Patents
A kind of method promoting spring cuckoo seed germination seedling Download PDFInfo
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- CN103947551B CN103947551B CN201410163710.6A CN201410163710A CN103947551B CN 103947551 B CN103947551 B CN 103947551B CN 201410163710 A CN201410163710 A CN 201410163710A CN 103947551 B CN103947551 B CN 103947551B
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Abstract
The invention discloses a kind of method promoting spring cuckoo seed germination seedling, comprising: the seed getting spring cuckoo, carry out disinfection with after water logging kind; After sterilization, described seed is inoculated in medium and carries out vernalization cultivation; After seed germination seedling, will continue in seedling replanting to matrix to cultivate; Is described medium for containing 0.4 ~ 0.6mg/L? the 1/4MS medium of KT and 0.03 ~ 0.06mg/LNAA, or is described medium for containing 1.8 ~ 2.2mg/L? GA
3with 0.03 ~ 0.06mg/L? the 1/4MS medium of NAA.Spring cuckoo seed is placed in medium cultivation and impels its germinating to seedling by the present invention, by adding the hormone of suitable kind, suitable concentration in the medium, the germinating time of spring cuckoo seed early, and spring cuckoo seed can reach higher germination rate in the short period of time, the usage amount of hormone is also few compared with the method for traditional seed soaking, saves cost.
Description
Technical field
The invention belongs to plant biotechnology field, is a kind of method promoting spring cuckoo seed germination seedling.
Background technology
Azalea is Ericaceae (Ericaceae) Rhododendron (Rhododendron) plant, is one of large famous flower of China ten.Azalea is one and belongs to greatly, and the whole world about has more than 1000 to plant, and maximum with Asia, and about have 850 kinds, wherein China just has more than 530 to plant.China azalea is of a great variety, aboundresources, and interspecific difference is larger.Greening is Woody flower with azalea, is a kind of evergreen dwarf shrub.According to the difference at florescence, spring cuckoo and Xia Juan can be divided into.Spring, cuckoo had very high ornamental value, large flower and brilliant color, and germination is strong, resistance to pruning.Plantation in blocks, its scenery is particularly grand.Be widely used in gardens, plant and all can form extremely beautiful scenery in grove, sylvan life, small stream limit, lawn and building are other.Potted plant and moulding cuckoo can be done simultaneously.
Spring cuckoo seed is very tiny, and thousand kernel weight only has about 0.05g, but each fruit and seed number is extremely many, about has 150-300 grain, widely different between different cultivars.The breeding of azalea is mainly based on cuttage, grafting.Seminal propagation is generally used for crossbreeding.The spring seed of cuckoo can carry out with adopting with broadcasting, and about 20-30 days is emerged.
In order to promote seed sprouting, the mode of general employing hormone seed soaking carries out vernalization, be placed in the hormone solution of finite concentration, certain kind (being generally gibberellin) by seed, after seed soaking a period of time (being generally 24 hours), then be placed in greenhouse and germinate.But this kind of mode germination rate is usually lower, and the working concentration of hormone is high, usage amount is large.
Seminal propagation is the important technical of conventional hybridization breeding, and crossbreed needs to be bred by seed.Azalea is woody plant, poor growth, at least needs triennium from being seeded into bloom.With the mode of adopting with broadcasting, can not the activity, bud ratio, growth potential etc. of accurate count hybrid seed, be used for judging the index of seed quality.If can solve the problem, theory and technology basis can not only be established for the seed sprouting and rooting of conventional hybridization breeding, the germination rate of hybrid seed can be improved simultaneously, hybrid seed be grown more healthy and strong, for solid foundation is established in the screening of filial generation.
Summary of the invention
The invention provides a kind of method promoting spring cuckoo seed germination seedling, can impel spring cuckoo seed germination within a short period of time, and Corticosteroids amount is few, germination rate is high.
Promote a method for spring cuckoo seed germination seedling, comprising:
(1) get the seed of spring cuckoo, carry out disinfection with after water logging kind;
(2), after sterilization, described seed is inoculated in medium and carries out vernalization cultivation;
(3), after seed germination seedling, will continue in seedling replanting to matrix to cultivate;
Described medium is the 1/4MS medium containing 0.4 ~ 0.6mg/LKT and 0.03 ~ 0.06mg/LNAA, or described medium is for containing 1.8 ~ 2.2mg/LGA
3with the 1/4MS medium of 0.03 ~ 0.06mg/LNAA.
Spring cuckoo seed is placed in medium cultivation and impels its germinating to seedling by the application, by adding the hormone of suitable kind, suitable concentration in the medium, early, spring cuckoo seed can reach higher germination rate to the germinating time of spring cuckoo seed in the short period of time, and the usage amount of hormone is few.
Kind, the germination impact of concentration on spring cuckoo seed of hormone are comparatively large, and test proves, different hormone kinds, and the time of spring cuckoo seed complete germination and the difference of germination rate are very large, even same hormone, the faint change of concentration also can have an impact to it.Preferably, in described medium, the concentration of described NAA is 0.05mg/L.
Preferably, the concentration of described KT is 0.5mg/L.Cultivate 26 day spring cuckoo seed can sprout completely, and germination rate can up to 70%.
Preferably, described GA
3concentration be 2.0mg/L.GA
3during for 2mg/L, cultivate 7 days, spring cuckoo seed starts to sprout, and cultivating 29 day spring cuckoo seed can sprout completely, and germination rate can up to 75%.
Described spring kind of cuckoo is ' The purple butterfly ', is ensure germination rate, and seed preferably takes from ripe uncracked green capsule.
Vernalization need first be soaked seed before cultivating, and seed soaking can impel spring cuckoo seed to germinate ahead of time, and during seed soaking, the temperature of water is generally 18 ~ 23 DEG C, and the time of seed soaking is 20 ~ 30 hours, and the temperature of water is 20 DEG C preferably, and the time of seed soaking is 24 hours preferably.
Suitable temperature and light is conducive to spring cuckoo seed germination according to condition, and generally, the temperature that described vernalization is cultivated is 22 ~ 25 DEG C, is preferably 24 DEG C.The illumination condition that described vernalization is cultivated is: intensity of illumination is 2000 ~ 2500lx, and light application time is 12 ~ 14h/d, and preferably, intensity of illumination is 2500lx, light application time is 12h/d.
When seedling grows to 3 ~ 5 true leaves, seedling replanting can be cultivated to nutritious peat soil, during cultivation, keep temperature to be 18 ~ 22 DEG C, humidity is 60 ~ 80%.
Compared with prior art, beneficial effect of the present invention is:
Spring cuckoo seed is placed in medium cultivation and impels its germinating to seedling by the present invention, by adding the hormone of suitable kind, suitable concentration in the medium, the germinating time of spring cuckoo seed early, and spring cuckoo seed can reach higher germination rate in the short period of time, the usage amount of hormone is also few compared with the method for traditional seed soaking, saves cost.
Method of the present invention is easy and simple to handle, and culture medium prescription is simple, for later crossbreeding work and Hybrid qualification provide technical support.
Accompanying drawing explanation
Fig. 1 is spring cuckoo seed germination figure.
Fig. 2 is embodiment 1 is embodiment 1 spring cuckoo sowing schematic diagram.
Fig. 3 is embodiment 1 for spring cuckoo seedling takes root situation.
Fig. 4 to be embodiment 1 be spring cuckoo seedling transfers to the growing state in import nursery peat soil.
Embodiment
Below in conjunction with embodiment, the present invention is further explained.
Embodiment 1
A kind of spring cuckoo the method for seed quick-speed germination seedling:
(1) seed preparation
Mid or late November, ripe at spring cuckoo ' The purple butterfly ' capsule, but be still uncracked green capsule time, adopted down, after liquid detergent water soaking 20-30min, under being placed on flowing water, rinsed 1-2h.Scrub the foul on capsule surface gently with banister brush after, blotting the moisture on capsule surface with filter paper, installing (without the need to covering culture dish lid) with culture dish.Being put into indoor is equipped with in the container of silica-gel desiccant, carries out drying, until capsule natural cracking.
(2) seed soaking
Before seed soaking, by the capsule strip off of cracking, without the need to carrying out disinfection to spring cuckoo seed.Select the seed of mature and plump after impurity elimination, place it in the warm water of 20 DEG C and soak 24h.
(3) medium preparation and sterilization
The medium of preparation containing hormon, horizontal high voltage sterilizing of going forward side by side (some hormone needs after autoclaving, joins in medium).
CK group:
①1/4MS②1/4MS+0.05mg/LNAA；
KT group:
①1/4MS+0.05mg/LNAA+0.5mg/LKT②1/4MS+0.05mg/LNAA+1.0mg/LKT③1/4MS+0.05mg/LNAA+2.0mg/LKT④1/4MS+0.5mg/LKT⑤1/4MS+1.0mg/LKT⑥1/4MS+2.0mg/LKT；
GA
3group:
①1/4MS+0.05mg/LNAA+0.15mg/LGA
3②1/4MS+0.05mg/LNAA+0.3mg/LGA
3③1/4MS+0.05mg/LNAA+0.6mg/LGA
3④1/4MS+0.05mg/LNAA+1.0mg/LGA
3⑤1/4MS+0.05mg/LNAA+2.0mg/LGA
3⑥1/4MS+0.15mg/LGA
3⑦1/4MS+0.3mg/LGA
3⑧1/4MS+0.6mg/LGA
3⑨1/4MS+1.0mg/LGA
3⑩1/4MS+2.0mg/LGA
3
(4) seed disinfection
Take out the seed soaked, blot the moisture of the surface of the seed with filter paper.First use 70% alcohol-pickled 30s, after aseptic water washing 3-5 time, seed is soaked in 15-18min in the mercuric chloride water of 0.1%, then uses aseptic water washing 3-5 time, each washing time is no less than 3min.Blot the moisture of the surface of the seed with filter paper after sterilization.
(5) aseptic seeding
The seed of sterilizing is inoculated in different culture media respectively, each process 30, repeats for 3 times.Be positioned over after inoculation that temperature is 24 DEG C, intensity of illumination is 2500lx, light application time is cultivate in the Sterile culture room of 12h/d.Observed and recorded seed germination time, germination rate, germination vigor.
(6) seedling replanting
The seedling with 2 cotyledons and 3-5 sheet true leaf is transferred to and is mixed with in the peat soil of carbendazim.Splendid attire is coiled in peat soil cave.Transplant seedlings 4-6 in each aperture, be conducive to the growth of seedling.Being placed on temperature after having transplanted seedlings is 20 DEG C, and humidity is 60%-80%, and light application time is continue in the artificial climate incubator of 12h/d to cultivate.The growth rate of the rear seedling of observation transplanting and growing state etc.
The germination rate of different disposal lower spring cuckoo seed, in table 1, table 2, table 3.
The impact of table 1CK group spring cuckoo seed germination
Table 2 hormone KT is on the impact (KT group) of spring cuckoo seed germination
Table 3GA
3group spring cuckoo seed germination rate (GA
3group)
As can be seen from above data, in 1/4MS medium, adding plant hormone or do not add plant hormone all can inducing sterile seed sprouting.Meanwhile, there were significant differences to the inducing effect of sterile seed germination for different hormone combinations.The inducing effect of some hormone combinations is not even as the blank of 1/4MS medium.0.05mg/LNAA, can sprout ahead of time by inducing sterile seed, improves seed germination rate simultaneously.GA
32.0mg/L+NAA0.05mg/L+1/4MS combines, and remarkable for improving spring cuckoo seed germination rate and accelerating seed germination speed effect, cultivate and within 7 days, start to sprout, within 29 days, sprout completely, germination rate is up to 75%.KT0.5+NAA0.05+1/4MS combination also has good effect, and cultivate and within 11 days, start to sprout, within 26 days, sprout completely, germination rate reaches 70%.
Comparative example
The detailed process of the spring cuckoo seed germination experiment in artificial climate incubator is as follows:
(1) seed collection is identical with embodiment 1.
(2) seed is peeled off from capsule (do not need sterilization), uses 10 respectively, 100,200,400,600, the GA of 800mg/L
3solution seed soaking, seed soaking time is 24h, with deionized water seed soaking 24h for contrast.More than process be designated as respectively process 1., process 2., process 3., process 4., process 5., process 6..Each process 50, repeats for 3 times.The seed processed, after blotting with filter paper, the culture dish be placed in using filter paper as germinating bed is cultivated, and deionized water is as culture matrix, and the moment ensures the moistening of filter paper.Cover culture dish lid to ensure humidity.And be placed in the artificial climate incubator that temperature is 20 DEG C, humidity is 80%-85%.Note moisture situation every day, keep the skin wet in time.Reject mouldy seed, and change clean filter paper in time.Observed and recorded seed germination time, germination rate, germination vigor.
(3) seedling replanting
When transferring them to containing in abundant organic peat soil after seed grows two panels true leaf, in 20 DEG C, humidity is 60%-80%, light application time is continue in the artificial climate incubator of 12h/d to cultivate, and observes the growth rate of seedling after transplanting and growing state etc.
Different GA
3under solution seed soaking process, first seed started rudiment at after planting the 8th day.
Under different disposal, the germination rate of spring cuckoo seed, in table 4.
Adopt conventional GA
3the mode of solution seed soaking, spring cuckoo seed starts the time of sprouting, time of sprouting completely is longer than or suitable with the mode of the application's medium culture, but germination rate is far below the present invention, and germination rate of the present invention can reach more than 70%, and this comparative example is only about 60%, and the GA that comparative example uses
3concentration high, the concentration of 400 ~ 800mg/L just can reach the germination rate of about 60%.
Claims (2)
1. promote a method for spring cuckoo seed germination seedling, it is characterized in that, comprising:
(1) get the seed of spring cuckoo, carry out disinfection with after water logging kind; The temperature of described water is 18 ~ 23 DEG C, and the time of described seed soaking is 20 ~ 30 hours;
(2), after sterilization, described seed is inoculated in medium and carries out vernalization cultivation; The temperature that described vernalization is cultivated is 22 ~ 25 DEG C, and illumination condition is: intensity of illumination is 2000 ~ 2500lx, and light application time is 12 ~ 14h/d;
Described medium is the 1/4MS medium containing 0.5mg/LKT and 0.05mg/LNAA, or described medium is for containing 2.0mg/LGA
3with the 1/4MS medium of 0.05mg/LNAA;
(3), after seed germination seedling, will continue in seedling replanting to peat soil matrix to cultivate; During described cultivation, keep temperature to be 18 ~ 22 DEG C, humidity is 60 ~ 80%.
2. the method for claim 1, is characterized in that, described seed takes from the ripe uncracked green capsule of spring cuckoo.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06189646A (en) * | 1992-12-25 | 1994-07-12 | New Oji Paper Co Ltd | Method for promoting rooting of culture shoot in plant of genus rhododendron |
| JPH06189643A (en) * | 1992-12-25 | 1994-07-12 | New Oji Paper Co Ltd | Large-scale multiplication of rhododendron |
| CN101695284A (en) * | 2009-10-29 | 2010-04-21 | 江苏省农业科学院 | Method for raising seedling rate of rhododendron hybrid seeds |
| CN102792893A (en) * | 2012-08-27 | 2012-11-28 | 云南农业大学 | Tissue culture propagating method of Rhododendron agastum |
| CN102919133A (en) * | 2012-12-03 | 2013-02-13 | 江苏省农业科学院 | Technical method for promoting tissue culture rooting of alpine rose amethyst |
-
2014
- 2014-04-22 CN CN201410163710.6A patent/CN103947551B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06189646A (en) * | 1992-12-25 | 1994-07-12 | New Oji Paper Co Ltd | Method for promoting rooting of culture shoot in plant of genus rhododendron |
| JPH06189643A (en) * | 1992-12-25 | 1994-07-12 | New Oji Paper Co Ltd | Large-scale multiplication of rhododendron |
| CN101695284A (en) * | 2009-10-29 | 2010-04-21 | 江苏省农业科学院 | Method for raising seedling rate of rhododendron hybrid seeds |
| CN102792893A (en) * | 2012-08-27 | 2012-11-28 | 云南农业大学 | Tissue culture propagating method of Rhododendron agastum |
| CN102919133A (en) * | 2012-12-03 | 2013-02-13 | 江苏省农业科学院 | Technical method for promoting tissue culture rooting of alpine rose amethyst |
Non-Patent Citations (3)
| Title |
|---|
| 丁洪玲."牛皮杜鹃组织培养及植株解剖学研究".《中国优秀硕士学位论文全文数据库 农业科技辑》.2010,(第 03 期), * |
| 刘燕等."大白杜鹃组培与快繁研究———基本外植体和初代培养基筛选".《种子》.2010,第29卷(第8期), * |
| 程雪梅等."马缨杜鹃的组织培养与快速繁殖".《植物生理学通讯》.2007,第44卷(第2期), * |
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