CN102757293B - Organic selenium-enzyme and preparation method thereof - Google Patents
Organic selenium-enzyme and preparation method thereof Download PDFInfo
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- CN102757293B CN102757293B CN2012102767930A CN201210276793A CN102757293B CN 102757293 B CN102757293 B CN 102757293B CN 2012102767930 A CN2012102767930 A CN 2012102767930A CN 201210276793 A CN201210276793 A CN 201210276793A CN 102757293 B CN102757293 B CN 102757293B
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- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- 239000000843 powder Substances 0.000 claims abstract description 23
- 240000002791 Brassica napus Species 0.000 claims abstract description 12
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 12
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 11
- 229930006000 Sucrose Natural products 0.000 claims abstract description 11
- 239000005720 sucrose Substances 0.000 claims abstract description 11
- 238000000034 method Methods 0.000 claims abstract description 4
- 102000004190 Enzymes Human genes 0.000 claims description 27
- 108090000790 Enzymes Proteins 0.000 claims description 27
- 235000004977 Brassica sinapistrum Nutrition 0.000 claims description 11
- RXHZUZCZBDBCQL-UHFFFAOYSA-N [K].[Se].P(O)(O)(O)=O Chemical compound [K].[Se].P(O)(O)(O)=O RXHZUZCZBDBCQL-UHFFFAOYSA-N 0.000 claims description 11
- 244000068988 Glycine max Species 0.000 claims description 10
- 235000010469 Glycine max Nutrition 0.000 claims description 10
- 238000004458 analytical method Methods 0.000 claims description 9
- 238000000855 fermentation Methods 0.000 claims description 6
- 230000004151 fermentation Effects 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 4
- 229910052711 selenium Inorganic materials 0.000 abstract description 15
- 239000002689 soil Substances 0.000 abstract description 13
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract description 8
- 239000003337 fertilizer Substances 0.000 abstract description 7
- 244000005700 microbiome Species 0.000 abstract description 6
- 239000000203 mixture Substances 0.000 abstract description 6
- 102000004169 proteins and genes Human genes 0.000 abstract description 4
- 108090000623 proteins and genes Proteins 0.000 abstract description 4
- 239000003864 humus Substances 0.000 abstract description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 2
- 235000011293 Brassica napus Nutrition 0.000 abstract 1
- 229920002261 Corn starch Polymers 0.000 abstract 1
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 abstract 1
- 102100022624 Glucoamylase Human genes 0.000 abstract 1
- 235000019764 Soybean Meal Nutrition 0.000 abstract 1
- 238000001816 cooling Methods 0.000 abstract 1
- 239000008120 corn starch Substances 0.000 abstract 1
- 238000007791 dehumidification Methods 0.000 abstract 1
- 230000002503 metabolic effect Effects 0.000 abstract 1
- 239000011785 micronutrient Substances 0.000 abstract 1
- 235000013369 micronutrients Nutrition 0.000 abstract 1
- 239000004455 soybean meal Substances 0.000 abstract 1
- 230000007306 turnover Effects 0.000 abstract 1
- 238000009849 vacuum degassing Methods 0.000 abstract 1
- 229910009112 xH2O Inorganic materials 0.000 abstract 1
- 239000011669 selenium Substances 0.000 description 14
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 13
- 229940091258 selenium supplement Drugs 0.000 description 13
- 238000006243 chemical reaction Methods 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 239000003643 water by type Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000004021 humic acid Substances 0.000 description 2
- 239000007858 starting material Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 229910052785 arsenic Inorganic materials 0.000 description 1
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000036983 biotransformation Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 229910052793 cadmium Inorganic materials 0.000 description 1
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 229910052804 chromium Inorganic materials 0.000 description 1
- 239000011651 chromium Substances 0.000 description 1
- 238000005056 compaction Methods 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical group [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 229910021432 inorganic complex Inorganic materials 0.000 description 1
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000007269 microbial metabolism Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 230000000529 probiotic effect Effects 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 229960001471 sodium selenite Drugs 0.000 description 1
- 235000015921 sodium selenite Nutrition 0.000 description 1
- 239000011781 sodium selenite Substances 0.000 description 1
- 238000004162 soil erosion Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
Landscapes
- Fertilizers (AREA)
- Soil Conditioners And Soil-Stabilizing Materials (AREA)
Abstract
The invention discloses an organic selenium-enzyme. The content of protein is 25-40%, the content of activated carbon is 8-9.55, humus is smaller than or equal to 50%, Se is larger than or equal to 5%, and H2O is smaller than or equal to 10%. The invention further discloses a preparation method of the organic selenium-enzyme. The method includes mixing, by weight, 60-70 parts of dregs of rapeseeds, 30-40 parts of soybean meal and corn starch with equal proportion, and 1-3 parts of sucrose with water at a temperature of 110-120 DEG C for 120 minutes; cooling the mixture until the temperature is 40-50 DEG C, mixing 0.5-1.5 parts of by weight of yeast powder, placing the mixture into a thermostat at a temperature of 45 DEG C for 120 hours; mixing 0.5-1.5 parts of by weight of glucoamylase and 3-6 parts of by weight of K3SeP3O10.xH2O into the mixture, placing the mixture into a culture pot, placing the culture pot in a sterile workshop for culture at a room temperature of 25-35 DEG C; andplacing the mixture in a pressure kettle for vacuum degassing and dehumidification after 30 days to obtain the organic selenium-enzyme. The organic selenium-enzyme can activate microorganisms in soil, physical and mechanical states of the soil are obviously improved through metabolic turnover of the microorganisms, and the organic selenium-enzyme can be used as a micronutrient fertilizer for improving the soil.
Description
Technical field
The invention provides a kind of organoselenium enzyme and preparation method thereof.
Background technology
Since 1974, China's agroforestry are produced a large amount of chemical fertilizer that use, and have caused the increasingly deterioration of soil organisms and edatope, have produced many adverse consequencess: 1. soil compaction, soil erosion and desertification, 2. the pollution of heavy metal, the 3. decline of farm crop interior quality; The underproduction of unit surface, 4. the pollution of environment is to coercing of human health etc.How to get along amiably and peacefully with nature in the development of modern agricultural production science and technology, the sustainable development of protection Limited Land resource, promotion multi-form agriculture is the problem that we will ponder deeply and expect to solve.
At present, the selenium fertilizer of selling on the market mostly is inorganic selenium, it is Sodium Selenite, the Use Limitation valency is low, and life-time service can be to water quality and environment, the preparation method of existing a kind of organic selenium enzyme preparation, use the probiotic bacteriums such as fungi to be carrier, method by bio-transformation is converted into the low conversion rate of organoselenium to inorganic selenium, only has 30%~60%, affects result of use.Application number is the preparation method that 200910231110.8 file has been announced a kind of organic selenium enzyme preparation, adopts bacterial classification to cultivate for three times, and inorganic selenium is converted into organoselenium, but low conversion rate, and residual inorganic selenium is harmful to for soil microorganisms.
The present invention relates to a kind of yeast powder, saccharifying enzyme used as starter, be that 200610136924X " the phosphoric acid selenium potassium mixture " announced is as catalyzer with the patent No., with the albumen selenium enzyme that powder ﹑ cake-shaped rape seed dregs precursor logical everfermentation ﹑ enzymolysis metabolism in shallow lake forms, it is used as little fertilizer of improveing soil.Utilize it to activate microorganism in soil and harmless to soil microorganisms, by the microbial metabolism conversion, make the soil physical and chemical states obtain obviously improvement.
Summary of the invention
Purpose of the present invention aims to provide a kind of organoselenium enzyme, and it can well be applied to soil improvement.
The objective of the invention is to realize by following manner: a kind of organoselenium enzyme, each component concentration is: protein is 25~40%, activated carbon is 8~9.5%, humus≤50%, Se 〉=5%, H
2O≤10%, described albumen selenium enzyme is brown ceramic powder, microscopically has glomerocryst to exist, and a kind of special ferment flavor is arranged; Solvend 〉=50%.
Another object of the present invention is to provide the preparation method of above-mentioned organoselenium enzyme, may further comprise the steps: with 60~70 weight part cake-shaped rape seed dregs, 30~40 weight parts by the analysis for soybean powder of equal proportion proportioning and W-Gum, 1~3 weight part sucrose, blunge evenly by 110~120 ℃, processed in 120 minutes; Be cooled to 40~50 ℃, admix 0.5~1.5 weight part yeast powder, enter 45 ℃ of thermostat containers, fermentation in 120 hours; In whipping process, 0.5~1.5 weight part saccharifying enzyme and 3~6 weight part phosphoric acid selenium potassium solutions are admixed wherein equably; The dress Culture basin is put sterile workshop training and is grown, and room temperature keeps 25~35 ℃, enters the autoclave pressure vacuum outgas after 30 days, dehumidifying forms.
The starter that the present invention selects is yeast powder, saccharifying enzyme, and catalyzer is phosphoric acid selenium potassium, and fermentation substrate is cake-shaped rape seed dregs, analysis for soybean powder, W-Gum, sucrose.
The invention has the beneficial effects as follows: organoselenium enzyme of the present invention can significantly be urged to reach the purpose of improving soil fertility with the soil microorganisms breeding, is of value to crop growth.
Embodiment
Embodiment 1.
Machine selenium enzyme of the present invention adopts raw material to be: 70 kilograms of the thin cake-shaped rape seed dregs of 100 orders, 15 kilograms of analysis for soybean powder, 15 kilograms of W-Gums, 2 kilograms of sucrose, 1.5 kilograms of yeast powders, 1.5 kilograms in saccharifying enzyme, 50 liters of clean waters, 5 kilograms in phosphoric acid selenium potassium.
Cake-shaped rape seed dregs, analysis for soybean powder, W-Gum, sucrose are blunged evenly by 120 ℃, 120 minutes pyroprocessing materials, be cooled to 50 ℃, admix yeast powder and enter 45 ℃ of thermostat containers, after fermentation in 120 hours, in stirring, saccharifying enzyme and phosphoric acid selenium potassium solution are admixed wherein equably, the dress Culture basin, put sterile workshop training and grow, room temperature keeps 25 ℃, enters the autoclave pressure vacuum outgas after 30 days, dehumidifying forms.
Organoselenium enzyme of the present invention is checked through Hunan Province Institute of Analysis, test basis is GB/T 8572-2010 " mensuration that contains nitrogen content in the composite fertilizer ", GB/T 23349-2009 " arsenic in the fertilizer, plumbous chromium, cadmium, the mensuration of mercury ecological index ", GB18877(5.10)-2009 " mensuration of total humic acid in the organic-inorganic complex fertilizer ", Interventions Requested are humus, water-insoluble, moisture content, activated carbon, selenium, protein, assay are that selenium is 6.44%, and water-insoluble is 63.49%, moisture content 9.82%, humic acids is 23.41%, and activated carbon is 8.91%, and protein is 29.63%.
Embodiment 2.
Machine selenium enzyme of the present invention adopts raw material to be: 60 kilograms of the thin cake-shaped rape seed dregs of 100 orders, 20 kilograms of analysis for soybean powder, 20 kilograms of W-Gums, 2 kilograms of sucrose, 1 kilogram of yeast powder, 1 kilogram in saccharifying enzyme, 50 liters of clean waters, 6 kilograms in phosphoric acid selenium potassium.
Cake-shaped rape seed dregs, analysis for soybean powder, W-Gum, sucrose are blunged evenly by 120 ℃, the pyroprocessing material was cooled to 50 ℃ in 120 minutes, admix yeast powder and enter 45 ℃ of thermostat containers, after fermentation in 120 hours, in stirring saccharifying enzyme and phosphoric acid selenium potassium solution are admixed wherein equably, the dress Culture basin is put the sterile workshop training and is grown, room temperature keeps 35 ℃, enters the autoclave pressure vacuum outgas after 30 days, dehumidifying forms.
Embodiment 3.
Organoselenium enzyme of the present invention adopts raw material to be: 70 kilograms of the thin cake-shaped rape seed dregs of 100 orders, 15 kilograms of analysis for soybean powder, 15 kilograms of W-Gums, 2 kilograms of sucrose, 0.8 kilogram of yeast powder, 0.7 kilogram in saccharifying enzyme, 50 liters of clean waters, 5 kilograms in phosphoric acid selenium potassium.
Cake-shaped rape seed dregs, analysis for soybean powder, W-Gum, sucrose are blunged evenly by 120 ℃, the pyroprocessing material was cooled to 50 ℃ in 120 minutes, admix yeast powder and enter 45 ℃ of thermostat containers, after fermentation in 120 hours, in stirring saccharifying enzyme and phosphoric acid selenium potassium solution are admixed wherein equably, the dress Culture basin is put the sterile workshop training and is grown, room temperature keeps 30 ℃, enters the autoclave pressure vacuum outgas after 30 days, dehumidifying forms.
The above; only for the better embodiment of the present invention, but protection scope of the present invention is not limited to this, anyly is familiar with those skilled in the art in the technical scope that the present invention discloses; the variation that can expect easily or replacement all should be encompassed within protection scope of the present invention.Therefore, protection scope of the present invention should be as the criterion with the protection domain of claims.
Claims (2)
1. the preparation method of an organoselenium enzyme is characterized in that, with the analysis for soybean powder of the equal proportion proportioning of 60~70 weight part cake-shaped rape seed dregs, 30~40 weight parts and W-Gum, 1~3 weight part sucrose, blunges evenly by 110~120 ℃, processes in 120 minutes; Be cooled to 40~50 ℃, admix 0.5~1.5 weight part yeast powder, 0.5~1.5 weight part saccharifying enzyme and 3~6 weight part phosphoric acid selenium potassium, enter 45 ℃ of thermostat containers, fermentation in 120 hours; The dress Culture basin is put sterile workshop training and is grown, and room temperature keeps 25~35 ℃, enters the autoclave pressure vacuum outgas after 30 days, dehumidifying forms.
2. the preparation method of a kind of organoselenium enzyme according to claim 1, it is characterized in that the weight part of material is preferably: 1~2 weight part sucrose, 0.8~1 weight part yeast powder, 0.5~0.8 weight part saccharifying enzyme, 4~5 weight part phosphoric acid selenium potassium, 70 weight part cake-shaped rape seed dregs, 15 weight part soya beans, 15 weight part W-Gums.
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CN105432319A (en) * | 2015-01-13 | 2016-03-30 | 罗大全 | Biological selenium-enriched fungi breeding method |
CN110317100A (en) * | 2019-07-22 | 2019-10-11 | 唐山津国农业技术开发有限公司 | The production technology of organic selenium Liquid Fertilizer |
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