CN102308851A - Bacillus thuringiensis insecticide produced by using sweet potato starch wastewater - Google Patents
Bacillus thuringiensis insecticide produced by using sweet potato starch wastewater Download PDFInfo
- Publication number
- CN102308851A CN102308851A CN2010102115707A CN201010211570A CN102308851A CN 102308851 A CN102308851 A CN 102308851A CN 2010102115707 A CN2010102115707 A CN 2010102115707A CN 201010211570 A CN201010211570 A CN 201010211570A CN 102308851 A CN102308851 A CN 102308851A
- Authority
- CN
- China
- Prior art keywords
- sweet potato
- potato starch
- liquid seeds
- wastewater
- waste water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a method for producing a bacillus thuringiensis (Bt) microbial insecticide by recycling sweet potato starch wastewater, belonging to the technical fields of microbial insecticides and recycling of wastewater. In the method, high-concentration wastewater produced in the production process of sweet potato starch is mainly taken as a main nutrient raw material for producing the Bt pesticide. The method comprises the following steps of: (1) preparing a sweet potato wastewater culture medium; (2) preparing liquid seeds; (3) performing liquid fermentation; and (4) performing post-treatment for preparing a needed dosage form. Due to the adoption of the method, an environmentally-friendly Bt microbial insecticide can be produced at low cost; and moreover, a method for efficiently recycling a large amount of high-concentration wastewater produced in the production process of sweet potato is provided, and pollutant discharge is reduced.
Description
Technical field:
The invention belongs to microbial pesticide and waste water reclaiming technical field.
Background technology:
China is maximum in the world sweet potato producing country, and the total cultivated area of sweet potato remains on about 6,200,000 hectares, and gross yield is stabilized in more than 100,000,000 tons, accounts for global 80%.In the crops main product in the whole nation, Ipomoea batatas is only second to paddy rice, wheat and corn, occupies the 4th.Sweet potato plantation less investment, output is many, and the edible dry matter of unit are occupies first of the various crops.The sweet potato strength of combatting natural disaster is strong, and is drought-enduring, impoverishment tolerant, and the mountain area, hills also can be planted, and also can obtain output preferably in the place of the difficult growth of other crops.It is very high that nutrient sweet potato is worth, and is one of World Health Organization's " optimum food " of choosing out, and many experts think, sweet potato is 21 century one of an optimal food, and simultaneously, sweet potato also is one of most important renewable energy resources raw material.
Sweet potato is perishable, unsuitable long-term storage.The deep processing of sweet potato can solve because of storing the improper phenomenon of mashed potato in a large number that causes of bright potato, and the sweet potato purified starch can be produced hundreds of value products through the processing of different depth, about 10-30 times of increment.But, present processing technology, 10 tons of left and right sides high concentrated organic wastewaters of the about output of 1 ton of sweet potato starch of every production; The COD value of these waste water is up to more than the 10000mg/L, and content of organics is the highest in the sweet potato cell sap after wherein starch separates, and COD can reach more than the 20000mg/L; Mainly contain dissolubility starch, protein, pectin, organic acid and a spot of grease; Deal with improperly, very easily putrefactive fermentation makes the water quality blackout smelly.And adopt the wastewater treatment qualified discharge of general sewage treatment process with high concentration like this, energy resource consumption is very big.Consider that from another angle the organic matter in the sweet potato starch waste water (comprising: protein, pectin, carbohydrate etc.) is the good nutrition substrate of growth of microorganism, can make microbiological culture media, the fermenting and producing microniological proudcts.
Bacillus thuringiensis (Bacillus thuringiensis is called for short Bt) is a kind of Gram-positive bacteria, belongs to bacillus, the bacterium of Lu Sheng habit.In addition, Bt can colonize on the larva of some moth classes and butterfly class, or even plant surface.Its thalline is a rod-short, gives birth to flagellum, single giving birth to or the formation short chain.It produces the desinsection parasporal crystal albumen that is called delta-endotoxin in the gemma forming process, these albumen have very high insecticidal activity.At present, confirmed tens of kinds of Bt fungus strains and more than 130 kind of insecticidal crystal protein, clone Bt gene has also changed plant over to, and in plant corpus, efficiently expresses.
The Bt insecticide is present output maximum, uses a quasi-microorganism insecticide the most widely.The cultural method of Bt has multiple, as: (application number: disclose a kind of production method of Bt medium 200410009911.7), its production technology is Chinese invention patent: in 1kg supporter (rice bran, husk, the corncob of fragmentation or the crop straw of chopping), add 10～15kg water, 0.2～0.8g cellulase; Using dilute sulfuric acid to regulate the pH value is 5～6, and control thermotonus temperature is 30～60 ℃, stirs 40～56 hours; The pH value was 6～7 after reaction finished, and added wheat bran 5～9kg, soybean cake powder 1～3kg; Corn flour 0.5～1.5, kg, dusty yeast 0.1～0.3; Kg stirs and promptly obtains the easy solid-state fermentation culture medium of pulverizing of Bt biopesticide.Chinese invention patent (application number: the Bt that discloses a kind of anti-locust 200610019564.5); And introduced its cultural method; It at first is shake flask fermentation; In the medium of 100 unit volumes, add starch, soybean cake powder, the sub-powder of cottonseed cake, peptone, dusty yeast, corn steep liquor, epsom salt, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, calcium carbonate in proportion, reach AMS, supplementing water; Next is a dress 100ml medium in triangular flask; Sterilization back inoculation Bt; Be cultured to bacillus exfoliation in uniform temperature; The 3rd is to adopt the medium that screens, and in automatic fermenter, ferments, and the acidifying of fermentation ends after fermentation liquid adds emulsifier, and ferment liquid concentrates the direct atomized drying in back and prepares wetting powder.Chinese invention patent (application number: disclose a kind of method of utilizing high-concentration waste organic water in fermentation industry to produce microorganism insecticide 99119084.X); Invention utilizes high-concentration waste organic water in fermentation industry (need pass through preliminary treatment sometimes) and cultivates in a large number in fermentation tank through the Bt bacterial classification of domestication; Again through zymotic fluid is suitably handled; Obtain the good liquid or solid-state microbial insecticide preparation of insecticidal effect; In low-cost processes waste water, can obtain the good microorganism insecticide of ecological benefits in a large number again, turn waste into wealth.At present, be that the method for medium large-scale culture Bt is not seen bibliographical information with sweet potato starch waste water.
Summary of the invention:
The purpose of this invention is to provide a kind of recycling sweet potato starch waste water and produce the method for Bt microorganism insecticide.
The production method of Bt insecticide of the present invention is following:
1, the preparation of sweet potato starch wastewater medium: in high concentration sweet potato starch waste water, add soybean cake powder 0.2%-1.2%, dusty yeast 0.2%-1.2% stirred, in 115-126 ℃ of sterilization 10-35 minute.
2, liquid seeds preparation: the Bt bacterial strain of inoculation slant preservation in the triangular flask that the sweet potato starch wastewater medium is housed, in 25-37 ℃, 120-200 rev/min shaken cultivation 24-48 hour, obtain 1 grade of liquid seeds; The inoculation volume ratio is 1 grade of liquid seeds of 1%-5% in the seeding tank that the sweet potato starch wastewater medium is housed, and in 26-35 ℃, logical filtrated air was cultivated 8-36 hour, obtained 2 grades of liquid seeds;
3, liquid state fermentation: the inoculation volume ratio is 2 grades of liquid seeds of 5%-15% in the fermentation tank that the sweet potato starch wastewater medium is housed, and in 26-35 ℃, logical filtrated air was cultivated 12-48 hour; According to national standard method sampling and measuring spore count and insecticidal effect, the toxicity of zymotic fluid is tired and can be reached more than the 3000IU/ μ l;
4, post processing: with cultured zymotic fluid through ultrafiltration and concentration 2-10 doubly, the conventional method according to the microbial pesticide preparation adds stabilizing agent, synergist again, and adds auxiliary agent according to the formulation needs.The lucifuge packing, the place preserves in the cool place.
Embodiment:
The Bt microorganism insecticide of being produced by technical scheme of the present invention not only has nontoxic, the eco-friendly characteristics of biopesticide; And its production cost is low, has effectively utilized waste water, reduces pollutant emission.Embodiment is following:
Embodiment 1: sweet potato starch waste water is produced the Bt wetting powder
1, the preparation of sweet potato starch wastewater medium: in high concentration sweet potato starch waste water (COD20-30g/L), add soybean cake powder 1.0%, dusty yeast 0.7% stirred, in 121 ℃ of sterilizations 15 minutes.
2, liquid seeds preparation: the Bt bacterial strain of inoculation slant preservation in the triangular flask that the sweet potato starch wastewater medium is housed, in 35 ℃, 150 rev/mins of shaken cultivation 48 hours obtain liquid seeds.
3, liquid state fermentation: the inoculation volume ratio is 5% liquid seeds in the fermentation tank that the sweet potato starch wastewater medium is housed, and in 35 ℃, logical filtrated air was cultivated 48 hours; According to national standard method sampling and measuring insecticidal effect, the toxicity of zymotic fluid is tired to cultivate when reaching 5000IU/ μ l and is finished.
4, post processing: through 3 times of ultrafiltration and concentration, the conventional method according to the microbial pesticide preparation adds stabilizing agent, synergist, wetting agent, filler again with cultured zymotic fluid, atomized drying, and the lucifuge packing, the place preserves in the cool place.
Embodiment 2: sweet potato starch waste water is produced the Bt aqua
1, the preparation of sweet potato starch wastewater medium: in high concentration potato sweet potato starch waste water (COD 20-30g/L), add soybean cake powder 1.0%, dusty yeast 0.5% stirred, in 121 ℃ of sterilizations 20 minutes.
2, liquid seeds preparation: the Bt bacterial strain of inoculation slant preservation in the triangular flask that the sweet potato starch wastewater medium is housed, in 35 ℃, 180 rev/mins of shaken cultivation 48 hours obtain liquid seeds.
3, liquid state fermentation: the inoculation volume ratio is 8% liquid seeds in the fermentation tank that the sweet potato starch wastewater medium is housed, and in 35 ℃, logical filtrated air was cultivated 48 hours; According to national standard method sampling and measuring insecticidal effect, the toxicity of zymotic fluid is tired to cultivate when reaching 3500IU/ μ l and is finished.
4, post processing: through 2 times of ultrafiltration and concentration, the conventional method according to the microbial pesticide preparation adds stabilizing agent, synergist, preservative, spreader-sticker, sticker again with cultured zymotic fluid, the lucifuge packing, and the place preserves in the cool place.
Claims (1)
1. one kind is utilized sweet potato starch waste water to produce bacillus thuringiensis (Bacillus thuringiensis; Abbreviation Bt) method of microorganism insecticide; It is characterized in that; This method is to be that the main medium raw material carries out liquid state fermentation and produces the Bt insecticide with the high-concentration waste water that produces in the sweet potato starch production process, comprises the steps:
1, the preparation of sweet potato starch wastewater medium: in high concentration sweet potato starch waste water, add soybean cake powder 0.2%-1.2%, dusty yeast 0.2%-1.2% stirred, in 115-126 ℃ of sterilization 10-35 minute;
2, liquid seeds preparation: the Bt bacterial strain of inoculation slant preservation in the triangular flask that the sweet potato starch wastewater medium is housed, in 25-37 ℃, 120-200 rev/min shaken cultivation 24-48 hour, obtain 1 grade of liquid seeds; The inoculation volume ratio is 1 grade of liquid seeds of 1%-5% in the seeding tank that the sweet potato starch wastewater medium is housed, and in 26-35 ℃, logical filtrated air was cultivated 8-36 hour, obtained 2 grades of liquid seeds;
3, liquid state fermentation: the inoculation volume ratio is 2 grades of liquid seeds of 5%-15% in the fermentation tank that the sweet potato starch wastewater medium is housed, and in 26-35 ℃, logical filtrated air was cultivated 12-48 hour; According to national standard method sampling and measuring spore count and insecticidal effect, the toxicity of zymotic fluid is tired and can be reached more than the 3000IU/ μ l;
4, post processing: with cultured zymotic fluid through ultrafiltration and concentration 2-10 doubly, the conventional method according to the microbial pesticide preparation adds stabilizing agent, synergist again, and adds auxiliary agent according to the formulation needs.The lucifuge packing, the place preserves in the cool place.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102115707A CN102308851A (en) | 2010-06-29 | 2010-06-29 | Bacillus thuringiensis insecticide produced by using sweet potato starch wastewater |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102115707A CN102308851A (en) | 2010-06-29 | 2010-06-29 | Bacillus thuringiensis insecticide produced by using sweet potato starch wastewater |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102308851A true CN102308851A (en) | 2012-01-11 |
Family
ID=45422719
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010102115707A Pending CN102308851A (en) | 2010-06-29 | 2010-06-29 | Bacillus thuringiensis insecticide produced by using sweet potato starch wastewater |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102308851A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105779323A (en) * | 2014-12-23 | 2016-07-20 | 中国科学院生态环境研究中心 | Bacillus subtilis capable of efficiently degrading quorum sensing signal molecules and production method of inoculant thereof |
| CN109652342A (en) * | 2019-02-01 | 2019-04-19 | 福建农林大学 | A method of Bt liquid fermentation medium is prepared by raw material of vegetable castoff |
| CN117467577A (en) * | 2022-10-16 | 2024-01-30 | 青岛科技大学 | Sweet potato paste fermentation product and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1246284A (en) * | 1999-09-14 | 2000-03-08 | 中国科学院生态环境研究中心 | Process for preparing microbe insecticide with high-concentration waste organic water in fermentation industry |
| CN1799363A (en) * | 2006-01-17 | 2006-07-12 | 北京大学 | Method for preparing bacillus thuringiensis microbiological pesticide by starch waste liquor |
-
2010
- 2010-06-29 CN CN2010102115707A patent/CN102308851A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1246284A (en) * | 1999-09-14 | 2000-03-08 | 中国科学院生态环境研究中心 | Process for preparing microbe insecticide with high-concentration waste organic water in fermentation industry |
| CN1799363A (en) * | 2006-01-17 | 2006-07-12 | 北京大学 | Method for preparing bacillus thuringiensis microbiological pesticide by starch waste liquor |
Non-Patent Citations (3)
| Title |
|---|
| 周学永,等: "利用工业废水生产生物农药苏云金杆菌", 《内蒙古石油化工》 * |
| 王丽芳: "以淀粉废液为原料生产生物农药", 《微生物学杂志》 * |
| 雷发懋,等: "微生物转化淀粉废水制备生物灭蚊剂", 《生态环境》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105779323A (en) * | 2014-12-23 | 2016-07-20 | 中国科学院生态环境研究中心 | Bacillus subtilis capable of efficiently degrading quorum sensing signal molecules and production method of inoculant thereof |
| CN109652342A (en) * | 2019-02-01 | 2019-04-19 | 福建农林大学 | A method of Bt liquid fermentation medium is prepared by raw material of vegetable castoff |
| CN117467577A (en) * | 2022-10-16 | 2024-01-30 | 青岛科技大学 | Sweet potato paste fermentation product and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101892157B (en) | Composite microbial inoculum for preparing biological bacterial fertilizer and preparation method thereof | |
| CN101659934B (en) | Antagonistic bacteria preventing and removing continuous cropping banana Panama wilt disease and microbial organic fertilizer thereof | |
| CN103113167B (en) | Compound microbial organic fertilizer and preparation method thereof | |
| CN106748341A (en) | Potato microbial bacterial manure special and preparation method thereof | |
| CN101734956B (en) | Method for preparing microorganism fermentation liquor from fish skins or fish scales and bacterial manure containing fermentation liquor | |
| CN104177137A (en) | Microbial straw rotting agent and preparation method thereof | |
| CN104293694A (en) | Preparation method for sludge aerobic composting composite inoculum | |
| CN103194405B (en) | Growth-promoting bacteria for promoting ginger growth and preventing and controlling continuous cropping ginger soil-borne wilt and microorganism organic fertilizer produced from growth-promoting bacteria | |
| CN101974428A (en) | Complex microbial preparation capable of resisting replant obstacle resistance and preparation method thereof | |
| CN101255402A (en) | Thallus for producing biological fertilizer by employing cane sugar filter mud fermentation | |
| CN102180729B (en) | Strontium-containing organic microorganism fertilizer and preparation method thereof | |
| CN101948780B (en) | Antagonist bacterium for preventing and treating continuous cropping hot pepper epidemic disease and microbial organic fertilizer thereof | |
| CN105130685A (en) | Preparation method for organic fertilizer containing Nu-polyglutamic acid | |
| CN107043280A (en) | A kind of biological compound fertilizer and preparation method thereof | |
| CN104371949A (en) | Root-knot nematode killing composite microbial inoculum and preparation method thereof | |
| CN108017446A (en) | The preparation method of enzyme containing activated carbon and the nutritional agents of microorganism cenobium | |
| CN104263679A (en) | High-efficiency phosphate-solubilizing bacteria and application thereof | |
| CN103387428A (en) | Preparation method for organic material decomposition agent | |
| CN108558484A (en) | A kind of selenium-enriched microbe fertilizer and preparation method thereof | |
| CN105198512B (en) | A kind of method that double-microbial fermentation prepares gamma-polyglutamic acid organic fertilizer | |
| CN107628894A (en) | Composite bacteria agent increase soil fertility and its preparation method and application | |
| CN102533594B (en) | Fermentation culture of Bacillus vallismortis and production method of compound microbial fertilizer | |
| CN106316659A (en) | Insect-resistant fertilizer filler with purple cabbage waste residues as main material and preparation method thereof | |
| CN103409351A (en) | Growth promoting strain used for promoting banana growth and microbial organic fertilizer produced with same | |
| CN102911905A (en) | Preparation method for microbial fertilizer fungicide |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20120111 |
|
| WD01 | Invention patent application deemed withdrawn after publication |