CN102726203B - Method for propagating radix tetrastigme underground officinal tubers - Google Patents
Method for propagating radix tetrastigme underground officinal tubers Download PDFInfo
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Abstract
The invention relates to a plant propagation method, and particularly relates to a method for propagating radix tetrastigme underground officinal tubers. The method comprises the following steps of: 1) low-temperature moisture preservation, sand storage and dormancy release; 2) germination acceleration and transplanting; and 3) large field transplanting. According to the invention, the radix tetrastigme underground officinal parts are utilized; through the technologies of low-temperature sand storage, dormancy release, greenhouse germination acceleration and large field transplanting, the radix tetrastigme underground officinal parts root after about 45 days. The method provided by the invention has the advantages of rapid propagation, low cost, high seedling rate, low corrosion rate and the like, thereby breaking through the technical defect that the original radix tetrastigme propagation technology is restricted to overground rattan cottage, which is easy for degradation, instable in variety characteristic, high in tissue culture investment, in need of professionals and the like.
Description
Technical field
The present invention relates to a kind of method for propagation, especially the underground medicinal rhizome part of radix tetrastigme propagation method.
Background technology
Radix tetrastigme (Tetrastigma hemsleyanum Diels et Gilg) is vitaceae, has another name called gold thread hoist, silk thread and hangs Jin Zhong, the flat rattan of three leaves, stone mouse, little flat rattan, stone monkey, soil through ball, three leaves pair.The main place of production in Southwestern China portion and Anhui, Zhejiang, Jiangxi, Fujian, Hubei, Hunan, Guangdong, Guangxi.Seldom, the wild reserves in Zhejiang Province are 2000 kilograms of left and right only for wild resource at present, and radix tetrastigme requires harsh to growing environment, generally be grown in the remote, thickly forested mountains of 700 meters of height above sea level and in the shade of overhanging cliff, will have scattered light above, side will have thin water to ooze out, and will have leaf to cover below; Growth rate is extremely slow, add in addition artificial crushing excavation, so output is now few, endangered.
Radix tetrastigme is with stem tuber and all herbal medicine, has clearing heat and detoxicatingly, and wind-expelling pain-stopping, activates blood circulation and disperses blood clots, the effects such as profit gasification phlegm, strengthening spleen and nourishing stomach.Be usually used in clinically treating hyperpyretic convulsion, rheumatism is ached, and irregular menstruation is coughed and breathed heavily phlegm, pharyngalgia scrofula, the diseases such as carbuncle furunculosis.To pneumonia, scrofula, hepatitis, the severe infections diseases such as gastritis, cervicitis, septicemia have obvious curative effects.The diseases such as Several Kinds of Malignancy and hypertension, cardiopathy, diabetes, neurasthenia are all had to extensive and good therapeutic action.
Modern medicine study shows: radix tetrastigme all has good therapeutic action to most primary carcinoma, metastatic carcinoma etc., all bad reactions that chemotherapy of tumors is brought, as low in appetite, vomiting, feel sick, epilation and leucocyte minimizing etc. all improve significantly, patients with terminal is had to alleviating pain effect, and it can also improve the immunologic function of human body effectively; And radix tetrastigme tool wide spectrum (applicable various solid tumors and non-patients with solid tumor are taken), efficiently (can improve rapidly carcinous heating, pain, chest ascites, leucocyte decline, apocleisis, the cachexia symptom such as weak, rapid-action, clinical total effective rate is high), nontoxic (is pure natural plant extract, without any side effects through testing), have no drug resistance (can be comprehensive, the inhibition tumour of too many levels, many target spots, completely different from the cytotoxicity of chemotherapeutics, the not anti-medicine situation of clinical application occurs).
Radix tetrastigme wild resource is very rare at present, belong to rare medicinal plant in imminent danger, radix tetrastigme propagation method is mainly concentrated the acrial part cuttage breeding method research of identification of lligera rhodantha hance in the past, but due to maternal few, the reproductive order of generation of radix tetrastigme too much after, be prone to quality deterioration phenomenon.And adopt the underground medicinal part reproductive effect of radix tetrastigme always undesirable, before main cause, way is directly planted in land for growing field crops by the underground stem tuber of radix tetrastigme, ignore the characteristic that the underground stem tuber of radix tetrastigme has dormancy, directly plantation causes the underground medicinal rhizome rotting rate of radix tetrastigme high, season is restricted, planting percent is low, never has a set of complete feasible high efficiency breeding technology.For protecting our wild radix tetrastigme resource in imminent danger, expand the population of radix tetrastigme, realize artificial high-quality radix tetrastigme industrialization plantation, become this traditional Chinese medicine expert and unanimously want the difficult problem solving.
Radix tetrastigme can pass through branch cutting, but must need a large amount of maternal resources, and can not be with the form of Miao Fanmiao, otherwise be easy to occur degradation phenomena, also can cultivate by tissue, but need professional and technical personnel and professional equipment, investment is large, difficult popularization.The radix tetrastigme of guaranteeing supply a large amount of is maternal, must be by setting up enough maternal regions, and utilizing the underground medicinal rhizome of radix tetrastigme of wild or artificial culture is one of best approach as material.
Summary of the invention
In order to solve above-mentioned technical problem, the object of this invention is to provide the propagation method of the underground medicinal rhizome of a kind of radix tetrastigme, that the method has is simple to operate, breeding fast, cost is low, planting percent is high, be easy to the advantages such as popularization.
In order to realize above-mentioned object, the present invention has adopted following technical scheme:
The propagation method of the underground medicinal rhizome of radix tetrastigme, the method comprises the following steps:
1) the husky Tibetan of low temperature moisturizing, breaking dormancy stage
The underground medicinal rhizome of fresh radix tetrastigme collecting is placed in the foam box that river sand is housed, on river sand, with wet gauze, cover, foam box covers with foam case lid, river sand humidity is controlled between 35%-45%, ambient temperature is controlled between 5-12 degree Celsius, this temperature section holding time be 30 days above;
2) vernalization, transplanting stage
Vernalization, when ambient temperature reaches more than 15 degrees Celsius, takes out radix tetrastigme in the cave dish that is transplanted to matrix, utilize green house, warm indoor temperature is controlled between 20-25 degree Celsius, keep 10-15 days, cave dish is upper to be covered with the gauze with wet, open gauze every day, depending on the humidity of stromal surface, when matrix is turned white, sprayer is sprayed water immediately, with sprayer to matrix water spray once, wait the bud length that radix tetrastigme grows to have 2-3 centimetre, remove immediately gauze;
3) field-transplanting
After the radix tetrastigme of vernalization has 85% plant leaf to have 3 leaf growths to open, can be transplanted to land for growing field crops by carrying substrates.
As further improvement, the concrete operations of above-mentioned step 1) are as follows:
1. first at the bottom of bubble chamber, cover the wet river sand of 1-2 centimetre, the underground medicinal rhizome of radix tetrastigme is evenly placed on river sand, at the underground medicinal rhizome of radix tetrastigme, cover the river sand of 3-5 centimetre again, and then put the underground medicinal rhizome of one deck radix tetrastigme, cover again river sand, when pushing up 3-5 centimetre apart from foam box, with river sand, radix tetrastigme is covered completely;
2. on river sand, with wet gauze, cover, the lid of foam box is covered to foam box;
3. low temperature moisturizing, breaking dormancy stage, if natural mean temperature was lower than 10 degrees Celsius at that time, foam box can be placed directly in the dark place of indoor cool place, lucifuge; If natural mean temperature was higher than 10 degrees Celsius at that time, foam box must be positioned in freezer, the temperature of freezer is arranged between 5-12 degree Celsius.
As further improvement, above-mentioned step 2) seedling medium formula by volume:
Humus soil: 60% ~ 65%
Peat: 35% ~ 30%
Sustained slow-release fertilizer: 2% ~ 3%
Ash 3% ~ 2%.
As further improvement, above-mentioned step 2) according to extraneous different temperatures, can adopt different disposal measure:
1. when natural world mean temperature of air is below 25 degrees Celsius, cave dish can be placed directly in green house, cave dish is upper to be covered with the gauze with wet, airtight green house, suitably heats, and greenhouse temperature is risen between 20-25 degree Celsius, open gauze every day, humidity situation depending on stromal surface is carried out moisturizing, when matrix, turns white, and sprays water immediately with sprayer;
2. work as natural world mean temperature of air more than 25 degrees Celsius, the processing of must sheltering from heat or light with the sunshade net covering green house of the rate of sheltering from heat or light 70%, all the other steps are same 1.;
3. pass through 15-20 days, radix tetrastigme will grow the bud of 2-3 centimetre, when having 80% radix tetrastigme to grow the bud of 2-3 centimetre, removes immediately the gauze removing immediately on the dish of cave.
As further improvement, above-mentioned step 3) is cultivated through vernalization, and 45th ~ 50 days, 90% radix tetrastigme can grow threeleaf loosestrife herb sheet, can go out garden field-transplanting or directly cultivate into maternal.
First the present invention carries out low temperature moisturizing preservation by the underground medicinal part of radix tetrastigme, breaking dormancy, avoiding previous methods is that the underground medicinal part of radix tetrastigme is directly planted in to large field, due to impacts such as the degenerating of extraneous weather, rainwater, causes a large amount of rotten situations to occur.In reproductive process, utilize the underground medicinal rhizome of radix tetrastigme to there is the physiological property of dormancy, by low temperature moisturizing, preserve, impel the composition of the part sprout inhibition containing to decompose; Second stage, in conjunction with the utilization of the conditions, particularly temperature such as suitable light, temperature, water, nutrient matrix, water, matrix, further improves survival rate and the germination rate of the underground medicinal rhizome of radix tetrastigme.
Adopt method provided by the invention to cultivate, by the low temperature moisturizing about 30 days, preserve, the hibernation feature of the underground medicinal rhizome of radix tetrastigme is disengaged, at the temperature of 20-25 degree Celsius, carry out vernalization, general 40-45 days, has 60% left and right to start rudiment, just has 80% rudiment about 50 days.About the 50th day, bastem originally grows to 2-3 centimetre, can remove the gauze that covers moisturizing, after waiting threeleaf loosestrife herb sheet all to trail, can go out garden and transplant.
The present invention can, by utilizing few maternal material to cultivate into greatest extent high-quality seedling, as planting of maternal, be to breed at present one of underground medicinal rhizome optimal path of radix tetrastigme.Utilize the underground medicinal part of radix tetrastigme, by husky breaking dormancy and booth vernalization, the field-transplanting technology of hiding of low temperature, through within approximately 45 days, taking root.This invention has the advantages such as breeding is fast, cost is low, planting percent is high, rotting rate is low, break through the original propagation technique of radix tetrastigme and be only confined to the cuttage of acrial part rattan, be prone to degeneration, breediness is unstable, tissue is cultivated the technological deficiency problem that investment is large, need professional etc.
Embodiment
The underground medicinal rhizome propagation method of radix tetrastigme, the method is undertaken by following following steps:
One, the husky Tibetan of low temperature moisturizing, breaking dormancy stage
A: choose thickness the wide * of long * high=55*35*30cm, thickness is at the foam box of 2 centimetres of left and right.Rugosity, at the river sand below 2 millimeters, is squirted with concentration 0.1% potassium permanganate, with Polypropylence Sheet, cover sterilization in 2-3 hour, then under air spacious 1-2 days or be exposed to the sun 1 day, then with clear water, mix wetly, humidity is controlled between 35%-45%;
B: first cover the wet river sand of 1-2 centimetre at the bottom of bubble chamber, the underground medicinal rhizome of radix tetrastigme is evenly placed on river sand, at the underground medicinal rhizome of radix tetrastigme, cover the river sand of 3-5 centimetre again, and then put the underground medicinal rhizome of one deck radix tetrastigme, cover again river sand, when pushing up 3-5 centimetre apart from foam box, with river sand, radix tetrastigme is covered completely;
C: cover with wet gauze on river sand, the lid of foam box is covered to foam box;
D: low temperature moisturizing, breaking dormancy stage, if natural mean temperature was lower than 10 degrees Celsius at that time, foam box can be placed directly in the dark place of indoor cool place, lucifuge.If natural mean temperature was higher than 10 degrees Celsius at that time, foam box must be positioned in freezer, the temperature of freezer is arranged between 5-12 degree Celsius.General through the low temperature holding time be 30 days above.
Two, vernalization, transplanting stage: vernalization is processed, and the underground medicinal rhizome of the radix tetrastigme through 1 month low temperature treatment is taken out from river sand, put into the cave dish that seedling medium is housed, the formula of seedling medium is (volume ratio):
Humus soil: 60%---65%
Peat: 35%---30%
Sustained slow-release fertilizer: 2%---3%
Ash 3%---2%
According to extraneous different temperatures, can adopt different disposal measure:
A: when natural world mean temperature of air is below 25 degrees Celsius, cave dish can be placed directly in green house, cave dish is upper to be covered with the gauze with wet, airtight green house, suitably heats, and greenhouse temperature is risen between 20-25 degree Celsius, open gauze every day, humidity situation depending on stromal surface is carried out moisturizing, when matrix, turns white, and sprays water immediately with sprayer.
B: when natural world mean temperature of air is more than 25 degrees Celsius, the processing of must sheltering from heat or light with the sunshade net covering green house of the rate of sheltering from heat or light 70%, the same 3A of all the other steps.
C: generally pass through 15-20 days, radix tetrastigme will grow the bud of 2-3 centimetre, when having 80% radix tetrastigme to grow the bud of 2-3 centimetre, removes the gauze removing immediately on the dish of cave immediately.
Three, field-transplanting technology: cultivate the 45th through vernalization---50 days, 90% radix tetrastigme can grow threeleaf loosestrife herb sheet, can go out garden field-transplanting or directly cultivate into maternal.
Claims (2)
1. the propagation method of the underground medicinal rhizome of radix tetrastigme, is characterized in that the method comprises the following steps:
1) the husky Tibetan of low temperature moisturizing, breaking dormancy stage
The underground medicinal rhizome of fresh radix tetrastigme collecting is placed in the foam box that river sand is housed, on river sand, with wet gauze, cover, foam box covers with foam case lid, river sand humidity is controlled between 35%-45%, ambient temperature is controlled between 5-12 degree Celsius, this temperature section holding time be 30 days above; Concrete operations are as follows:
1.1) first at the bottom of bubble chamber, cover the wet river sand of 1-2 centimetre, the underground medicinal rhizome of radix tetrastigme is evenly placed on river sand, at the underground medicinal rhizome of radix tetrastigme, cover the river sand of 3-5 centimetre again, and then put the underground medicinal rhizome of one deck radix tetrastigme, cover again river sand, when pushing up 3-5 centimetre apart from foam box, with river sand, radix tetrastigme is covered completely;
1.2) on river sand, with wet gauze, cover, the lid of foam box is covered to foam box;
1.3) low temperature moisturizing, breaking dormancy stage, if natural mean temperature was lower than 10 degrees Celsius at that time, foam box is placed directly in the dark place of indoor cool place, lucifuge; If natural mean temperature was higher than 10 degrees Celsius at that time, foam box must be positioned in freezer, the temperature of freezer is arranged between 5-12 degree Celsius;
2) vernalization, transplanting stage
Vernalization, when ambient temperature reaches more than 15 degrees Celsius, radix tetrastigme is taken out in the cave dish that is transplanted to matrix to seedling medium formula humus soil by volume: 60% ~ 65%, peat: 35% ~ 30%, sustained slow-release fertilizer: 2% ~ 3%, ash 2% ~ 3%; Utilize green house, warm indoor temperature is controlled between 20-25 degree Celsius, keep 10-15 days, cave dish is upper to be covered with wet gauze, opens gauze every day, humidity depending on stromal surface, when matrix is turned white, sprayer water spray, sprays water once to matrix with sprayer immediately, the bud length growing Deng radix tetrastigme has 2-3 centimetre, removes immediately gauze; According to extraneous different temperatures, adopt different disposal measure:
2.1) when natural world mean temperature of air is below 25 degrees Celsius, cave dish is placed directly in green house, cave dish is upper to be covered with the gauze with wet, airtight green house, suitably heats, and greenhouse temperature is risen between 20-25 degree Celsius, open gauze every day, humidity situation depending on stromal surface is carried out moisturizing, when matrix, turns white, and sprays water immediately with sprayer;
2.2) when natural world mean temperature of air is more than 25 degrees Celsius, the processing of must sheltering from heat or light with the sunshade net covering green house of the rate of sheltering from heat or light 70%, all the other steps are with 2.1);
2.3) through 15-20 days, radix tetrastigme will grow the bud of 2-3 centimetre, when having 80% radix tetrastigme to grow the bud of 2-3 centimetre, removes immediately the gauze on the dish of cave;
3) field-transplanting
After the radix tetrastigme of vernalization has 85% plant leaf to have 3 leaf growths to open, can be transplanted to land for growing field crops by carrying substrates.
2. the propagation method of the underground medicinal rhizome of radix tetrastigme according to claim 1, is characterized in that step 3) is through vernalization cultivation, and 45th ~ 50 days, 90% radix tetrastigme can grow threeleaf loosestrife herb sheet, can go out garden field-transplanting or directly cultivate into maternal.
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Families Citing this family (12)
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| CN102919013A (en) * | 2012-10-31 | 2013-02-13 | 丽水市汉方生物科技有限公司 | High-efficiency imitative wild cultivation method for Tetrastigma hemsleyanum Diels et Gilg |
| CN103004436B (en) * | 2012-12-18 | 2014-10-15 | 胡馨尹 | Method for planting tetrastigma hemsleyanum dielset gilg |
| CN103127314A (en) * | 2013-02-25 | 2013-06-05 | 胡馨尹 | Hemsley rockvine root tuber used for treating cancer fever |
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| CN104782416B (en) * | 2015-03-30 | 2018-02-06 | 浙江省林业科学研究院 | A kind of radix tetrastigme nursery plantation substrate |
| CN104770178B (en) * | 2015-04-17 | 2017-01-11 | 浙江农林大学 | Cuttage seedling culture method for root tuber early-growing of tetrastigma hemsleyanum |
| CN105505982A (en) * | 2016-01-12 | 2016-04-20 | 丽水学院 | Tissue culture method for brassica juncea |
| CN106718579A (en) * | 2016-12-21 | 2017-05-31 | 阜阳市颍泉区玉寿种植专业合作社 | A kind of cutting processing method of pear tree |
| CN107032876A (en) * | 2017-03-13 | 2017-08-11 | 丽水市农业科学研究院 | A kind of radix tetrastigme underground root tuber growth formula fertilizer and its matching method |
| CN110771302A (en) * | 2019-10-25 | 2020-02-11 | 青海省农林科学院 | Method for rapidly promoting germination of jerusalem artichoke tubers after dormancy breaking |
| CN112789965A (en) * | 2021-01-14 | 2021-05-14 | 上饶师范学院 | Method for promoting germination of radix tetrastigme seeds |
| CN116098035A (en) * | 2023-03-17 | 2023-05-12 | 毕节市中药研究所 | Heat-preserving germination accelerating matrix and germination accelerating method thereof |
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