CN102715168A - Sterilization combination containing phenylate diazole and azoxystrobin and application thereof - Google Patents
Sterilization combination containing phenylate diazole and azoxystrobin and application thereof Download PDFInfo
- Publication number
- CN102715168A CN102715168A CN2012102189875A CN201210218987A CN102715168A CN 102715168 A CN102715168 A CN 102715168A CN 2012102189875 A CN2012102189875 A CN 2012102189875A CN 201210218987 A CN201210218987 A CN 201210218987A CN 102715168 A CN102715168 A CN 102715168A
- Authority
- CN
- China
- Prior art keywords
- bactericidal composition
- phenylate
- azoles
- bactericide
- fluoxastrobin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
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- 238000004659 sterilization and disinfection Methods 0.000 title abstract 4
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- 239000003643 water by type Substances 0.000 description 1
Abstract
The invention relates to a sterilization combination and application thereof. The sterilization combination contains phenylate diazole and azoxystrobin and is used for controlling wheat powdery mildew, rust disease, rice blast and vegetable downy mildew. Compared with a single agent, the sterilization combination has remarkable synergistic effect on agricultural diseases, and is favorable for avoiding and delaying generation of drug resistance of germs.
Description
[technical field]
The invention belongs to technical field of pesticide.More specifically, the present invention relates to a kind of bactericidal composition that contains two azoles of phenylate and Fluoxastrobin, also relate to the purposes of said bactericidal composition.
[background technology]
Wheat powdery mildew and rust are the important diseases on the Wheat Production, can cause heavy losses to Wheat Production.Wheat powdery mildew (Blumeria graminis) is a kind of worldwide disease, in each main wheat producing countries distribution is arranged all, and China's Shandong coastal waters, Sichuan, Guizhou, Yunnan take place general, and it is also heavy to cause harm.In recent years should disease northeastward, North China, northwest Mai Qu, the gesture that is on the rise is also arranged.This disease can be encroached on each organ of wheat plant overground part, but is main with blade and leaf sheath, and grain husk shell and awns also can be injured when morbidity was heavy.Seedling stage to becoming the strain phase all can cause harm, main harm blade, also can cause harm when serious leaf sheath, stem stalk and fringe portion.General face of blade scab is more than reverse side, and lower blade is more than upper blade, and scab can heal for a long time in flakes.And cause yellow leaf withered.After stem stalk and leaf sheath were injured, plant was prone to lodging; Wheat rust is jaundice again, and 3 kinds of yellow rust, black stem rust and leaf rust are arranged.Be that distribution is wide on China's Wheat Production, propagation is fast, the important disease that hazard area is big.Wherein take place the most general and serious with stripe rust of wheat.After the wheat generation rust, nutrient is absorbed in the body, and chlorophyll is destroyed, and a large amount of soruss are broken through wheat leaf, straw epidermis, have a strong impact on wheat yield and quality.
Rice blast is the important disease on the paddy rice, and rice blast takes place heavier, especially general and serious with the high-yield field generation of dense planting hybrid rice of short stem in the Yangtze river basin and southern rice district.Mainly cause that by Deuteromycotina Rhizoctonia solani Kuhn sick (Rhizoctonia solani) disease is expanded from bottom to top, can arrive sword-like leave when serious, in addition the morbidity of fringe portion, large stretch of lodging.The heavier field piece of falling ill, the general underproduction 5% ~ 10% seriously can reach 50% ~ 70%.Along with the popularization of multi-spike-type kind of short stem, plant the raising of density and Fertilization Level, become an outstanding problem of rice high yield stable yields.
Vegetables downy mildew is one type of most important disease on the protection ground vegetables, can cause the underproduction 20%～30% generally speaking, can reach more than 50% when serious, and along with the expansion of protecting Di Peng chamber area, its harm is more and more heavy.
The inventor discovers; With two azoles of phenylate and Fluoxastrobin is the bactericidal composition of active ingredient, is used for wheat powdery mildew, rust, the control of rice blast and vegetables downy mildew; Can delay the drug-fast generation of germ; Improving actual preventive effect, enlarge fungicidal spectrum and reduce drug cost, is the important means of disease integrated control.
[summary of the invention]
[technical problem that will solve]
The purpose of this invention is to provide a kind of bactericidal composition that contains two azoles of phenylate and Fluoxastrobin.
Another object of the present invention provides the purposes of said bactericidal composition.
[technical scheme]
The present invention realizes through following technical proposals.
The present invention relates to a kind of bactericidal composition that contains two azoles of phenylate and Fluoxastrobin.This bactericidal composition contains following active component A and B:
A is the two azoles of phenylate with following structural formula:
The two azoles of phenylate (English common name: Difenodiconazole), chemical name: 2-[2-chloro-4-(4-chlorobenzene oxygen) phenyl]-1, two (1H-1,2,4-the triazol-1-yl)-2-propyl alcohol of 3-.CN 200610140836.7 discloses chemical structural formula, preparation method and the sterilizing use of the two azoles of phenylate, in present specification, this patent application is incorporated herein by reference in full.
B is a Fluoxastrobin.
Fluoxastrobin (Azoxystrobin) is methoxy acrylate (Strobilurin) series bactericidal agent; Efficiently, wide spectrum, nearly all mycota (Ascomycotina, Basidiomycotina, Mastigomycotina and Deuteromycotina) disease such as powdery mildew, rust, glume blight, net blotch, downy mildew, rice blast etc. are all had good activity.Can be used for cauline leaf spraying, seed treatment, also can carry out soil treatment, be mainly used in cereal, paddy rice, peanut, grape, potato, fruit tree, vegetables, coffee, lawn etc.
In bactericidal composition of the present invention, the weight ratio of said component A and B component is 1:100 ~ 100:1.
Preferably, the weight ratio of component A and B component is 1:50～50:1 in this bactericidal composition.
More preferably, the weight ratio of component A and B component is 1:20～20:1 in this bactericidal composition.
The invention still further relates to bactericidal composition of the present invention in control wheat powdery mildew, rust, the purposes in rice blast and the vegetables downy mildew.
The present invention relates to a kind of bactericide.
This bactericide contains the above-mentioned bactericidal composition of 0.1～90 weight % and surplus is acceptable carrier and an auxiliary agent on the agricultural chemicals.
Preferably, this bactericide contains the above-mentioned bactericidal composition of 10～80 weight %.
More preferably, this bactericide contains the above-mentioned bactericidal composition of 28～62 weight %.
Except as otherwise noted, " % " involved in the present invention is " percentage by weight ".
Can bactericide involved in the present invention be mixed with agricultural and go up acceptable forms, preferred dosage form is suspending agent, aqueous emulsion, microemulsion, wetting powder or water dispersible granules.
All these formulations all adopt method as well known to those skilled in the art to prepare by acceptable carrier and auxiliary agent on above-mentioned bactericidal composition and the agricultural chemicals.
As for the water-dispersible grain formulation, those skilled in the art can adopt conventional preparation method to be obtained by these the main auxiliary agent preparations of dispersant, wetting agent, disintegrant, binding agent and filler.
Described dispersant is selected for use from polycarboxylate, lignosulfonates or alkylnaphthalene sulfonate.
Described wetting agent is selected from polyoxyethylene alcohol, alkyl sulfate, alkylsulfonate or naphthalene sulfonate.
Described disintegrant is selected from ammonium sulfate, urea, sucrose, glucose, citric acid, succinic acid or sodium bicarbonate.
Described binding agent is selected from diatomite, corn starch, PVA, carboxymethyl (ethyl) cellulose family and microcrystalline cellulose class.
Described filler is selected from diatomite, kaolin, white carbon, fine particle calcium carbonate, sepiolite, talcum powder, attapulgite or potter's clay etc.
Employed dispersant, wetting agent, disintegrant, binding agent and filler all are product solds in the market when preparation water-dispersible grain formulation.
As for the wettable powder formulation, those skilled in the art can adopt conventional preparation method to be obtained by the main auxiliary agent preparation of dispersant, wetting agent and filler.
Described dispersant is selected from polycarboxylate, lignosulfonates or alkylnaphthalene sulfonate.
Described wetting agent is selected from alkyl sulfate, alkylsulfonate or naphthalene sulfonate.
Described filler is selected from diatomite, kaolin, white carbon, fine particle calcium carbonate, talcum powder, attapulgite or potter's clay etc.
Employed dispersant, wetting agent and filler all are product solds in the market when preparation wettable powder formulation.
As for suspending agent type, those skilled in the art can adopt conventional preparation method to be obtained by these auxiliary agent preparations of dispersant, wetting agent, thickener, preservative, antifoaming agent and antifreezing agent.
Described dispersant is selected from polycarboxylate, lignosulfonates, alkylnaphthalene sulfonate or TERSPERSE2425 (U.S. Huntsman Corporation produces, the alkyl naphthalene sulfonic acid salt).
Described wetting agent is selected from alkylphenol-polyethenoxy and gathers basic ether formaldehyde condensation products sulphate, alkylphenol-polyethenoxy base ether phosphate, phenethyl phenol polyethenoxy base ether phosphate, alkyl sulfate, alkylsulfonate, naphthalene sulfonate or TERSPERSE2500 (U.S. Huntsman Corporation product).
Described thickener is selected from xanthans, aluminium-magnesium silicate or bentonite.
Described preservative is selected from benzoic acid, Sodium Benzoate or BIT (1, the 2-benzisothiazole-3-ketone).
Described antifoaming agent is silicone based antifoaming agent.
Described antifreezing agent is selected from ethylene glycol, propane diols, glycerine, urea or inorganic salts such as sodium chloride.
Preparation during suspending agent type employed dispersant, wetting agent, thickener, preservative, antifoaming agent and antifreezing agent all be product sold in the market.
As for the water and milk formulation, those skilled in the art can adopt conventional preparation method to be obtained by these the main auxiliary agent preparations of emulsifier, solvent, stabilizing agent, antifreezing agent, thickener and preservative.
Described emulsifier is selected from Nonyl pheno (EO=10) ether phosphate, triphen ethyl-phenol polyoxyethylene ether phosphate (agricultural newborn 600# phosphate), Nongru-700 #, agricultural newborn 2201#, Span-60#, emulsifier T-60, TX-10, agricultural newborn 1601#, agricultural newborn 600# or agricultural newborn 400#.
Described solvent is selected from xylol, toluene, cyclohexanone or solvent naphtha (trade mark: S-150, S-180, S-200).
Described stabilizing agent is selected from triphenyl phosphite or epoxychloropropane.
Described antifreezing agent is selected from ethylene glycol, propane diols, glycerine, urea or inorganic salts such as sodium chloride.
Described thickener is selected from xanthans, bentonite or aluminium-magnesium silicate.
Described preservative is selected from BIT (1, the 2-benzisothiazole-3-ketone), benzoic acid or Sodium Benzoate.
Employed emulsifier, solvent, stabilizing agent, antifreezing agent, thickener and preservative all are product solds in the market when preparation water and milk formulation.
As for the micro emulsion formulation, those skilled in the art can adopt conventional preparation method to be obtained by these the main auxiliary agent preparations of emulsifier, cosolvent, solvent and stabilizing agent.
Described emulsifier is selected from calcium dodecyl benzene sulfonate (agricultural newborn 500#), Nongru-700 #, agricultural newborn 2201#, this dish-60#, Tween-80, TX-10, farming breast 1601, agricultural newborn 600# or agricultural newborn 400#.
Described cosolvent is selected from methyl alcohol, isopropyl alcohol, n-butanol or ethanol.
Described solvent is selected from cyclohexanone, N-Methyl pyrrolidone, xylol, toluene or solvent naphtha (trade mark: S-150, S-180, S-200).
Described stabilizing agent is selected from triphenyl phosphite or epoxychloropropane.
Employed emulsifier, cosolvent, solvent and stabilizing agent all are product solds in the market when preparation micro emulsion formulation.
Bactericidal composition of the present invention can provide with the finished product preparation form, and promptly each material mixes in the composition; Also can provide, in bucket (jar), directly mix before using with single agent formulation.Concentrate of the present invention mixes the concentration that obtains required active substance usually with water.
The invention still further relates to a kind of control wheat powdery mildew, rust; The method of rice blast and vegetables downy mildew; Before or after wheat, paddy rice or vegetable crop introduced disease, use above-mentioned bactericidal composition of the present invention or bactericide to the place of its growth or storage.Its application process is the conventional application process of agricultural chemicals, for example waters, sprays, spraying, dusting, broadcasts sowing etc.
[beneficial effect]
The present invention has following beneficial effect:
(1) compare with single agent, bactericidal composition according to the invention is to control wheat powdery mildew, rust, and rice blast and vegetables downy mildew disease have the synergistic effect of highly significant, have improved bactericidal effect;
(2) reduce the medication number of times, reduce drug cost, reduce environmental pollution and residue of pesticide;
(3) composition is made up of the active ingredient of different effects mechanism, and the action site increase helps overcoming and delays the drug-fast generation of germ.
[embodiment]
Can understand the present invention better through following embodiment.As not having specified otherwise, the active ingredient ratio among the present invention is weight ratio.
One, biological activity determination
Embodiment 1: two azoles of phenylate and azoxystrobin compound are to the Toxicity Determination of wheat powdery mildew
Test target wheat powdery mildew (Erysiphe graminis) is cultivated by the long-term live body of this laboratory and is selected for use susceptible variety (raising wheat No. 6) potted plant; Treat that seedling grows to 2 leaves～3 leaf phases; Spraying is handled the back and is dried naturally, and the fresh spore of white powder germ that the wheat leaf blade of falling ill was produced in last 24 hour is evenly shaken off to be inoculated on the potted plant wheat seedling of 2～3 leaf phases that chemicals treatment crosses.Postvaccinal examination material moves to 20 ℃, relative moisture 80% left and right sides controllable greenhouse glass room is interior cultivates.Note keeping basin soil moistening every day, investigate according to the classification of blank incidence about 7 days.With reference to pesticide field efficacy medicine test criterion () bactericidal agent for preventing and treating cereal class powdery mildew.
The disease grade scale is following:
0 grade: anosis;
1 grade: lesion area accounts for below 5% of one-piece blade area;
3 grades: lesion area accounts for 6% ~ 15% of one-piece blade area;
5 grades: lesion area accounts for 16% ~ 25% of one-piece blade area;
7 grades: lesion area accounts for 26% ~ 50% of one-piece blade area;
9 grades: lesion area accounts for more than 50% of one-piece blade area.
Preventive effect and co-toxicity coefficient computational methods are following:
On the basis of chemical control efficient, carry out the data analysis statistics with " DPS data handling system " 3.11 professional versions, obtain regression line, EC
50, correlation coefficient.EC that each that will measure according to the abundant method of Sun Yun handled
50Be converted into actual poison exponent (ATI); According to the proportioning of mixture, calculate theoretical poison exponent (TTI), calculate the co-toxicity coefficient (CTC) of mixture by following formula.
Theory is used the content (%) of toxicity index * A in using with of toxicity index (TTI)=A with
The content (%) of toxicity index * B of+B in using with
If co-toxicity coefficient, shows two azoles of phenylate and fluoxastrobin mixture greater than 120 synergistic effect is arranged; If co-toxicity coefficient is starkly lower than 100, show that its mixture is an antagonism; If co-toxicity coefficient is 100～120, show that its mixture is a summation action.
Two azoles of said phenylate and azoxystrobin compound are listed in the table 1 the Toxicity Determination result of wheat powdery mildew.
Table 1: two azoles of phenylate and azoxystrobin compound are to the Toxicity Determination result of wheat powdery mildew
| Medicament | EC 50（mg/L） | ATI | TTI | Co-toxicity coefficient CTC |
| The two azoles (A) of phenylate | 7.43 | 45.09 | ?- | - |
| Fluoxastrobin (B) | 3.35 | 100.00 | ?- | - |
| A:B（100:1） | 5.42 | 61.81 | 45.63 | 135.45 |
| A:B（50:1） | 4.85 | 69.07 | 46.16 | 149.62 |
| A:B（20:1） | 4.16 | 80.53 | 47.70 | 168.82 |
| A:B（10:1） | 4.03 | 83.13 | 50.08 | 165.99 |
| A:B（5:1） | 3.22 | 104.04 | 54.24 | 191.81 |
| A:B（1:1） | 2.48 | 135.08 | 72.54 | 186.21 |
| A:B（1:5） | 2.13 | 157.28 | 90.85 | 173.12 |
| A:B（1:10） | 2.07 | 161.84 | 95.01 | 170.34 |
| A:B（1:20） | 1.98 | 169.19 | 97.39 | 173.73 |
| A:B（1:50） | 2.21 | 151.58 | 98.92 | 153.23 |
| A:B（1:100） | 2.36 | 141.95 | 99.46 | 142.73 |
Embodiment 2: two azoles of phenylate and azoxystrobin compound are to the Toxicity Determination of wheat rust
Experiment target is designated as wheat leaf rust pathogen (Puccinia recondita), and wheat breed is for raising wheat No. 6, and every basin is selected about l0 strain wheat seeding for use; Treat long subsequent use to the 3-4 leaf phase; Supply reagent agent and proportioning medicament to be mixed with the soup that presets concentration, 4 repetitions are established in the processing of spraying, every processing.Get the wheat leaf blade that covers with the leaf rust bacterium behind the 24h, wash gently with sterile water and get surperficial fresh spore, filter with double gauze, processing spore concentration is the suspension about 100,000/ml, spray inoculation (pressure 0.1MPa).Wheat seeding was preserved moisture one day under 20 ℃ of conditions after connecing bacterium, put back to the greenhouse again, when waiting to contrast abundant morbidity after the week, carried out state of an illness investigation by field control effectiveness test criterion grade scale, referred to calculate preventive effect by disease.Obtain EC with return law of the straight line
50
The disease grade scale is following:
0 grade: anosis;
1 grade: lesion area accounts for below 5% of one-piece blade area;
3 grades: lesion area accounts for 6% ~ 25% of one-piece blade area;
5 grades: lesion area accounts for 26% ~ 50% of one-piece blade area;
7 grades: lesion area accounts for 51% ~ 75% of one-piece blade area;
9 grades: lesion area accounts for more than 76% of one-piece blade area.
Preventive effect and co-toxicity coefficient computational methods are following:
On the basis of chemical control efficient, carry out the data analysis statistics with " DPS data handling system " 3.11 professional versions, obtain regression line, EC
50, correlation coefficient.EC that each that will measure according to the abundant method of Sun Yun handled
50Be converted into actual poison exponent (ATI); According to the proportioning of mixture, calculate theoretical poison exponent (TTI), calculate the co-toxicity coefficient (CTC) of mixture by following formula.
Theory is used the content (%) of toxicity index * A in using with of toxicity index (TTI)=A with
The content (%) of toxicity index * B of+B in using with
If co-toxicity coefficient, shows two azoles of phenylate and fluoxastrobin mixture greater than 120 synergistic effect is arranged; If co-toxicity coefficient is starkly lower than 100 (below 80), show that its mixture is an antagonism; If co-toxicity coefficient is 100～120, show that its mixture is a summation action.
Two azoles of said phenylate and azoxystrobin compound are listed in the table 2 the Toxicity Determination result of wheat rust.
Table 2: two azoles of phenylate and azoxystrobin compound are to the Toxicity Determination result of wheat powdery mildew
| Medicament | EC 50（mg/L） | ATI | TTI | Co-toxicity coefficient CTC |
| The two azoles (A) of phenylate | 38.16 | 30.32 | - | - |
| Fluoxastrobin (B) | 11.57 | 100.00 | - | - |
| A:B（100:1） | 27.33 | 42.33 | 31.01 | 136.52 |
| A:B（50:1） | 24.92 | 46.43 | 31.69 | 146.53 |
| A:B（20:1） | 21.16 | 54.68 | 33.64 | 162.55 |
| A:B（10:1） | 18.21 | 63.54 | 36.65 | 173.34 |
| A:B（5:1） | 15.78 | 73.32 | 41.93 | 174.85 |
| A:B（1:1） | 10.42 | 111.04 | 65.16 | 170.41 |
| A:B（1:5） | 8.17 | 141.62 | 88.39 | 160.22 |
| A:B（1:10） | 6.93 | 166.96 | 93.67 | 178.25 |
| A:B（1:20） | 7.49 | 154.47 | 96.68 | 159.77 |
| A:B（1:50） | 8.27 | 139.90 | 98.63 | 141.84 |
| A:B（1:100） | 9.19 | 125.90 | 99.31 | 126.77 |
Embodiment 3: two azoles of phenylate and azoxystrobin compound are to the Toxicity Determination of rice blast
Experiment target is designated as rice blast (Pyricularia oryzae), and test adopts the co-toxicity coefficient method to estimate the co-toxicity effect of complex preparation, clearly than reasonable proportioning.
Two azoles of phenylate and Fluoxastrobin are dissolved with DMF respectively, be mixed with the soup of series concentration again with 0.1% Tween-80 aqueous solution dilution, the more two azoles of phenylate are processed preset series concentration compound solution with Fluoxastrobin respectively by different proportionings.With the rice blast pathogen of cultivating 8 days, break into the bacterium piece with the card punch of diameter 5mm at colony edge, with transfer needle the bacterium piece is moved to the toxic PDA medium central authorities that are mixed with in advance, place cultivation in 25 ℃ of incubators then, every processing repetition 4 times.After 8 days, adopt the right-angled intersection method to measure and respectively handle colony diameter cm, obtain the correction bacteriostasis rate with slide calliper rule.Two diameters are surveyed in each bacterium colony right-angled intersection, represent the bacterium colony size with its mean.Obtain bacteriostasis rate by following formula: colony growth inhibiting rate %=(the blank bacterium colony increases diameter-chemicals treatment bacterium colony and increases diameter) * 100/ blank bacterium colony increases diameter.
On the basis of medicament bacteriostasis rate, carry out the data analysis statistics with " DPS data handling system ", obtain regression line, EC
50, correlation coefficient.EC that each that will measure according to Sun Yunpei (Y-P Sun) method handled
50Be converted into actual poison exponent (ATI); According to the proportioning of mixture, calculate theoretical poison exponent (TTI), calculate the co-toxicity coefficient (CTC) of mixture by following formula.
Theory is used the content (%) of toxicity index * B in using with of content (the %)+B of toxicity index * A in using with of toxicity index (TTI)=A with
If co-toxicity coefficient, shows two azoles of phenylate and Fluoxastrobin mixture greater than 120 synergistic effect is arranged; If co-toxicity coefficient is starkly lower than 100, show that its mixture is an antagonism; If co-toxicity coefficient is 100～120, show that its mixture is a summation action.
Two azoles of said phenylate and azoxystrobin compound are listed in the table 3 Toxicity Determination of rice blast.
Table 3: two azoles of phenylate and azoxystrobin compound are to the Toxicity Determination result of rice blast
| Medicament | EC 50（mg/L） | ATI | TTI | Co-toxicity coefficient CTC |
| The two azoles (A) of phenylate | 3.15 | 56.51 | - | - |
| Fluoxastrobin (B) | 1.78 | 100.00 | - | - |
| A:B（100:1） | 2.32 | 76.72 | 56.94 | 134.75 |
| A:B（50:1） | 2.24 | 79.46 | 57.36 | 138.53 |
| A:B（20:1） | 1.98 | 89.90 | 58.58 | 153.47 |
| A:B（10:1） | 1.74 | 102.30 | 60.46 | 169.20 |
| A:B（5:1） | 1.55 | 114.84 | 63.76 | 180.12 |
| A:B（1:1） | 1.24 | 143.55 | 78.25 | 183.44 |
| A:B（1:5） | 1.12 | 158.93 | 92.75 | 171.35 |
| A:B（1:10） | 0.98 | 181.63 | 96.05 | 189.11 |
| A:B（1:20） | 1.17 | 152.14 | 97.93 | 155.35 |
| A:B（1:50） | 1.24 | 143.55 | 99.15 | 144.78 |
| A:B（1:100） | 1.32 | 134.85 | 99.57 | 135.43 |
Embodiment 4: two azoles of phenylate and azoxystrobin compound are to the Toxicity Determination of vegetables downy mildew
Subjects: Chinese cabbage downy mildew (Peronospora parasitica).
Test method: with reference to " the agricultural industry criteria NY/T 1156.2-2006 of the People's Republic of China (PRC) " mycelial growth rate method.Each medicament is established 5 dosage respectively by active constituent content and is handled; Above-mentioned each pathogen is used the PDA medium culture; When treating that bacterium colony has just covered with culture dish, break into the bacterium piece at colony edge, the bacterium piece is moved to the toxic PDA medium central authorities that are mixed with in advance with transfer needle with the card punch of diameter 5mm; Place then in 25 ℃ of incubators and cultivate every processing repetition 4 times.Look CK colony growth situation, adopt the right-angled intersection method to measure and respectively handle colony diameter cm, obtain to proofread and correct and suppress percentage with slide calliper rule.The EC of each medicament is obtained in linear regression analysis between the probit value through inhibiting rate and the logarithm value of series concentration
50Value is calculated co-toxicity coefficient (CTC) according to the abundant method of Sun Yun again.
Drug effect is calculated: two diameters are surveyed in each bacterium colony right-angled intersection, represent the bacterium colony size with its mean.Obtain the colony growth inhibiting rate by following formula:
Colony growth inhibiting rate %=(the blank bacterium colony increases diameter-chemicals treatment bacterium colony and increases diameter) * 100/ blank bacterium colony increases diameter
The co-toxicity coefficient computational methods are identical with biologicall test embodiment 1.
Table 4: two azoles of phenylate and azoxystrobin compound are to the Toxicity Determination result of Chinese cabbage downy mildew
| Medicament | EC 50（mg/L） | ATI | TTI | Co-toxicity coefficient CTC |
| The two azoles (A) of phenylate | 5.37 | 68.16 | - | - |
| Fluoxastrobin (B) | 3.66 | 100.00 | - | - |
| ?A:B（100:1） | 4.07 | 89.93 | 68.47 | 131.33 |
| ?A:B（50:1） | 3.88 | 94.33 | 68.78 | 137.15 |
| ?A:B（20:1） | 3.12 | 117.31 | 69.67 | 168.37 |
| ?A:B（10:1） | 2.95 | 124.07 | 71.05 | 174.62 |
| ?A:B（5:1） | 2.82 | 129.79 | 73.46 | 176.67 |
| ?A:B（1:1） | 2.28 | 160.53 | 84.08 | 190.92 |
| ?A:B（1:5） | 2.13 | 171.83 | 94.69 | 181.46 |
| ?A:B（1:10） | 2.29 | 159.83 | 97.11 | 164.59 |
| ?A:B（1:20） | 2.12 | 172.64 | 98.48 | 175.30 |
| ?A:B（1:50） | 2.48 | 147.58 | 99.38 | 148.51 |
| ?A:B（1:100） | 2.65 | 138.11 | 99.68 | 138.55 |
Can be known by above-mentioned Toxicity Determination result and to find out that two azoles of phenylate and Fluoxastrobin are mixed, in mass ratio was the scope of 100:1～1:100, to wheat powdery mildew, rust, rice blast and vegetables downy mildew all showed as synergistic effect.Wherein mass ratio is that synergistic effect is the most obvious in the scope of 20:1～1:20.These results show that two azoles of phenylate and Fluoxastrobin are mixed and prevent and treat wheat powdery mildew, rust, and rice blast and vegetables downy mildew have reasonability and feasibility.
Two, preparation embodiment
Percentage composition is the percentage by weight in said total formulation weight amount in all preparation proportionings.
Embodiment 1: the preparation water dispersible granules
Concrete preparation method is following: press formula rate; Former medicine and dispersant, wetting agent, binding agent, filler etc. are mixed, behind comminution by gas stream, obtain pulvis, add a certain amount of water and mix; In comminutor, carry out granulation, dry then, screening promptly gets granular product.
The two azoles azoxystrobin water dispersible granule preparations of 45% phenylate:
Take by weighing the two former medicines of azoles of 25% phenylate, the former medicine of 20% Fluoxastrobin, 3%NNO (alkylnaphthalene sulfonate formaldehyde condensation products), 2% Nekal BX (nekal), 4%K-12 (lauryl sodium sulfate), 5% carboxymethyl (ethyl) cellulose, 3% diatomite, 5% glucose, kaolin and add to 100%.Mix, behind comminution by gas stream, granulate.
Embodiment 2: the preparation suspending agent
Concrete preparation method is following: pressing formula rate, is medium with water, former medicine, dispersant, suspending agent and antifreezing agent etc. is joined shear 30min in the high-shear emulsion machine, use the sand mill sand milling again after, obtain suspending agent.
The two azoles Fluoxastrobin suspending agent preparations of 45% phenylate:
Take by weighing the two former medicines of azoles of 25% phenylate, the former medicine of 20% Fluoxastrobin, 3%FS3000 (phosphate type anion surfactant), 2%SXC (sulfonic acid type anion surfactant), 0.1% xanthans, 1% aluminium-magnesium silicate, 5% ethylene glycol, 0.15%BIT (1; The 2-benzisothiazole-3-ketone), 0.3% organically-modified silicone antifoam agent, deionized water adds to 100%.Process slurry, carry out sand milling, obtain product.
The two azoles Fluoxastrobin suspending agent preparations of 30% phenylate
Take by weighing the two former medicines of azoles of 5% phenylate, the former medicine of 25% Fluoxastrobin, 4%FS3000,1%JFC (AEO), 0.2% xanthans, aluminium-magnesium silicate 1%, 5% ethylene glycol, 0.3% benzoic acid, 0.5% organically-modified silicone antifoam agent, deionized water adds to 100%.Process slurry, carry out sand milling, obtain product.
Embodiment 3: the preparation wetting powder
Concrete preparation method is following: press formula rate, various auxiliary agents, filler, former medicine are mixed after after the Roughpulverizer pulverizing, get into airslide disintegrating mill and fully pulverize the mixed wetting powder that gets.
The two azoles Fluoxastrobin wetting powder preparations of 60% phenylate:
Take by weighing the two former medicines of azoles of 50% phenylate, the former medicine of 10% Fluoxastrobin, 3%NNO, 2% Nekal BX, 5% white carbon, fine particle calcium carbonate and add to 100%.Mix, obtain product after the pulverizing.
The two azoles Fluoxastrobin wetting powder preparations of 80% phenylate:
Take by weighing the two former medicines of azoles of 40% phenylate, the former medicine of 40% Fluoxastrobin, 5% calcium lignosulfonate, 2% Nekal BX, 1%K-12, kaolin and add to 100%.Mix, obtain product after the pulverizing.
Embodiment 4: the preparation microemulsion
The two azoles Fluoxastrobin microemulsion preparations of 25% phenylate:
Take by weighing the two former medicines of azoles of 15% phenylate, the former medicine of 10% Fluoxastrobin, 5% Nongru-700 #, 5% agricultural newborn 500#, 6% agricultural newborn 1601#, 15% cyclohexanone, 5%N-methyl pyrrolidone, 5% n-butanol, through dissolving fully and mixing, deionized water adds to 100%.After stirring, obtain product.
Embodiment 5: the preparation aqueous emulsion
The two azoles aqueous emulsion preparations of 10% Fluoxastrobin phenylate:
Take by weighing the two former medicines of azoles of 5% phenylate, the former medicine of 5% Fluoxastrobin, 3% agricultural newborn 2201#, 1.5% Nonyl pheno (EO=10) ether phosphate, 2.5% agricultural newborn 600# acid phosphatide, 20% cyclohexanone, 10% solvent naphtha S150, mixed dissolution obtains oil phase.With 5% ethylene glycol, 0.3% xanthans, 0.5% benzoic acid, deionized water adds to 100%, is made into water.Oil phase adds aqueous phase and fully mixes, and behind high-shear emulsifying, obtains product.
Three, field application test
Application implementation example 1: control wheat powdery mildew field control effectiveness test
The sub-district of test medicine, contrast medicament and blank is handled and is adopted district's group arrangement at random, sub-district area 20 square meters, 4 repetitions, each spraying control of booting stage and flowering initial stage 1 time.Five point samplings are at random adopted in the 7th day and investigation in 15 days of this test dispenser for the second time, get 20 strains at every, investigate last 3 leaves (having boot leaf then to comprise boot leaf) of every strain.
The disease grade scale is following:
0 grade: no scab;
1 grade: lesion area accounts for whole below 5% of leaf area;
3 grades: lesion area accounts for 6% ~ 15% of whole leaf area;
5 grades: lesion area accounts for 16% ~ 25% of whole leaf area;
7 grades: lesion area accounts for 26% ~ 50% of whole leaf area;
9 grades: lesion area accounts for whole more than 51% of leaf area.
Drug effect computational methods:, calculate disease index and preventive effect according to following (1), (2) formula according to investigation result.
In the formula:
CK
0, CK
1Be respectively the forward and backward disease index of blank district dispenser;
PT
0, PT
1Be respectively the forward and backward disease index of chemicals treatment district medicine.
Control wheat powdery mildew field control effectiveness test result lists in table 5.
Table 5: control wheat powdery mildew field control effectiveness test result
Application implementation example 2: prevent and treat the wheat rust field control effectiveness test
The sub-district of test medicine, contrast medicament and blank is handled and is adopted district's group arrangement at random, sub-district area 20 square meters, and 4 repetitions, this tests dispenser at the initial stage of a disease 1 time.30 days investigation drug effects are taked 5 samplings of every sub-district diagonal behind the medicine during investigation, and 20 leaves of every some investigation amount to 100 leaves, according to the sick level of stage division record different disposal, calculate disease index and control efficiency.
The disease grade scale is following:
0 grade: anosis
1 grade: lesion area accounts for below 5% of one-piece blade area;
3 grades: lesion area accounts for 6%～25% of one-piece blade area:
5 grades: lesion area accounts for the 26%-50% of one-piece blade area;
7 grades: lesion area accounts for 51%.75% of one-piece blade area;
9 grades: lesion area accounts for more than 76% of one-piece blade area.
According to investigation result, calculate disease index and preventive effect according to following (1), (2) formula.
In the formula:
CK
0, CK
1Be respectively the forward and backward disease index of blank district dispenser;
PT
0, PT
1Be respectively the forward and backward disease index of chemicals treatment district medicine.
Prevent and treat wheat rust field control effectiveness test result and list in table 6.
Table 6: prevent and treat wheat rust field control effectiveness test result
Application implementation example 3: control rice blast field control effectiveness test
The sub-district of test medicine, contrast medicament and blank is handled and is adopted district's group arrangement at random, sub-district area 20m
2, 4 repetitions, this test amounts to twice of dispenser.Be in paddy rice before heading stage during dispenser for the first time, full heading time is executed medicine for the second time.5 samplings of diagonal are adopted in every experimental plot, and every some investigation 50 strains more than every some investigation of fringe pest 50 fringes, are the unit record classification with the fringe, record disease grade.
The disease grade scale is following:
0 grade: anosis;
1 grade: every fringe loss 5% is (morbidity of indivedual branch stalk) once;
3 grades: every fringe loss 6% ~ 20% (branch stalk morbidity about 1/3);
5 grades: every fringe loss 21% ~ 50% (fringe neck or main shaft morbidity grain half are flat);
7 grades: every fringe loss 51% ~ 70% (morbidity of fringe neck, big portion shrivelled kernel);
9 grades: every fringe loss 71% ~ 100% (morbidity of fringe neck causes dead ears).
The drug effect computational methods:
According to investigation result, calculate disease index and preventive effect according to following (1), (2) formula.
In the formula:
CK
0, CK
1Be respectively the forward and backward disease index of blank district dispenser;
PT
0, PT
1Be respectively the forward and backward disease index of chemicals treatment district medicine.
Control rice blast field control effectiveness test result lists in table 7.
Table 7: control rice blast field control effectiveness test result
Application implementation example 4: control Chinese cabbage downy mildew field control effectiveness test
Regulation according to medicine inspecting institute of the Ministry of Agriculture " GB/T 17980.115-2004 pesticide field efficacy medicine test criterion ()---bactericidal agent for preventing and treating Chinese cabbage downy mildew "; The sub-district of test medicine, contrast medicament and blank is handled and is adopted district's group arrangement at random, sub-district area 15m
2, 4 repetitions, the dispenser for the first time of disease initial stage, 7d secondary dispenser at interval, the 7d investigation incidence of disease, disease index behind the secondary medicine.5 samplings of diagonal are adopted in every experimental plot, investigate 3 ~ 4 strains at every, investigate whole blades of every strain, record disease grade.
The disease grade scale is following:
0 grade: no scab;
1 grade: lesion area accounts for whole below 5% of leaf area;
3 grades: lesion area accounts for 6% ~ 10% of whole leaf area;
5 grades: lesion area accounts for 11% ~ 20% of whole leaf area;
7 grades: lesion area accounts for 21% ~ 50% of whole leaf area;
9 grades: lesion area accounts for whole more than 51% of leaf area.
The drug effect computational methods:
According to investigation result, calculate disease index and preventive effect according to following (1), (2) formula.
In the formula:
CK
0, CK
1Be respectively the forward and backward disease index of blank district dispenser;
PT
0, PT
1Be respectively the forward and backward disease index of chemicals treatment district medicine.
Control Chinese cabbage downy mildew field control effectiveness test result lists in table 8.
Table 8: control Chinese cabbage downy mildew field control effectiveness test result
Comprehensively above-mentioned, a large amount of indoor biometricses and field control effectiveness test result show that the beneficial effect that bactericidal composition according to the invention produces is:
(1) compare with single agent, this bactericidal composition has significant synergistic effect to disease, has improved bactericidal effect;
(2) reduce the medication number of times, lower drug cost, reduce environmental pollution and residue of pesticide;
(3) composition is made up of the active ingredient of different effects mechanism, and the action site increase helps overcoming and delays the drug-fast generation of germ.The present composition does not all have obvious harmful effect to trial crops, and leaf look, growing way etc. are all normal, and safety is good, meets the security requirement of pesticidal preparations.
Bactericidal composition according to the invention is to wheat powdery mildew, rust, and rice blast and vegetables downy mildew have good preventive effect.
Claims (8)
1. bactericidal composition that contains two azoles of phenylate and Fluoxastrobin is characterized in that this bactericidal composition contains following active component A and B:
A is the two azoles of phenylate with following structural formula:
B is a Fluoxastrobin;
Wherein the weight ratio of component A and B component is 1:100 ~ 100:1.
2. bactericidal composition according to claim 1 is characterized in that the weight ratio of component A and B component is 1:50～50:1 in this bactericidal composition.
3. bactericidal composition according to claim 1 is characterized in that the weight ratio of component A and B component is 1:20～20:1 in this bactericidal composition.
4. bactericide is characterized in that this bactericide contains that the described bactericidal composition of each claim and surplus are acceptable carrier and auxiliary agent on the agricultural chemicals among the claim 1-3 of 0.1～90 weight %.
5. according to the bactericide of claim 4, it is characterized in that this bactericide contains the described bactericidal composition of each claim among the claim 1-3 of 10～80 weight %.
6. according to the bactericide of claim 4, it is characterized in that this bactericide contains the described bactericidal composition of each claim among the claim 1-3 of 28～62 weight %.
7. according to the described bactericide of each claim among the claim 4-6, it is characterized in that this bactericide is mixed with suspending agent, aqueous emulsion, microemulsion, missible oil, wetting powder or water dispersible granules.
8. preventing and treating wheat powdery mildew, rust, the purposes in rice blast and the vegetables downy mildew according to the said bactericidal composition of each claim among the claim 1-4.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014082881A1 (en) | 2012-11-27 | 2014-06-05 | Basf Se | Substituted 2-[phenoxy-phenyl]-1-[1,2,4]triazol-1-yl-ethanol compounds and their use as fungicides |
| WO2014082871A1 (en) | 2012-11-27 | 2014-06-05 | Basf Se | Substituted 2-[phenoxy-phenyl]-1-[1,2,4]triazol-1-yl-ethanol compounds and their use as fungicides |
| EP2783569A1 (en) * | 2013-03-28 | 2014-10-01 | Basf Se | Compositions comprising a triazole compound |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0897664A1 (en) * | 1997-08-22 | 1999-02-24 | Chimac-Agriphar S.A. | Fungicidal composition |
| CN1511144A (en) * | 2001-06-13 | 2004-07-07 | Process for preparation of azoxystrobin and analogues thereof | |
| CN1923819A (en) * | 2006-10-12 | 2007-03-07 | 青岛科技大学 | compound containing aromatic ether and ditriazole and use thereof |
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2012
- 2012-06-28 CN CN 201210218987 patent/CN102715168B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0897664A1 (en) * | 1997-08-22 | 1999-02-24 | Chimac-Agriphar S.A. | Fungicidal composition |
| CN1511144A (en) * | 2001-06-13 | 2004-07-07 | Process for preparation of azoxystrobin and analogues thereof | |
| CN1923819A (en) * | 2006-10-12 | 2007-03-07 | 青岛科技大学 | compound containing aromatic ether and ditriazole and use thereof |
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| Title |
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| 《J. Agric. Food Chem.》 20090705 GUAN-PING YU et al Synthesis and Fungicidal Evaluation of 2-Arylphenyl Ether-3-(1H-1,2,4-triazol-1-yl)propan-2-ol Derivatives 第4854-4860页 1-8 第57卷, * |
| GUAN-PING YU ET AL: "Synthesis and Fungicidal Evaluation of 2-Arylphenyl Ether-3-(1H-1,2,4-triazol-1-yl)propan-2-ol Derivatives", 《J. AGRIC. FOOD CHEM.》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014082881A1 (en) | 2012-11-27 | 2014-06-05 | Basf Se | Substituted 2-[phenoxy-phenyl]-1-[1,2,4]triazol-1-yl-ethanol compounds and their use as fungicides |
| WO2014082871A1 (en) | 2012-11-27 | 2014-06-05 | Basf Se | Substituted 2-[phenoxy-phenyl]-1-[1,2,4]triazol-1-yl-ethanol compounds and their use as fungicides |
| EP2783569A1 (en) * | 2013-03-28 | 2014-10-01 | Basf Se | Compositions comprising a triazole compound |
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