CN102703562A

CN102703562A - Method for prompting extraction of linaloe oil by utilizing flora fermentation

Info

Publication number: CN102703562A
Application number: CN2012101975090A
Authority: CN
Inventors: 唐显
Original assignee: Individual
Current assignee: Individual
Priority date: 2012-06-15
Filing date: 2012-06-15
Publication date: 2012-10-03

Abstract

The invention discloses a method for prompting the extraction of linaloe oil by utilizing flora fermentation. By using the method, on the basis of not increasing the supply of a raw material, the unit yield of the linaloe oil can be greatly improved, and an agilawood material is subjected to mixed fermentation and conversion treatment through extracting and culturing a flora preparation by utilizing agilawood endophyte, so as to obviously promote the extraction rate of the linaloe oil. The method comprises the following culture steps of: extracting the agilawood endophyte, purifying a cultured primary solid test-tube strain, culturing a secondary strain of a flora by a combinative liquid shaking table, culturing an expanded tertiary liquid strain of a seed of the flora through a shaking table, and fermenting with the agilawood material in a mixed way. The extraction rate of the converted agilawood material is greatly improved on an original basis, so that the yield of the linaloe oil is increased from average 0.23% to 0.48% on the basis of not increasing the supply of an agilawood raw material; not only are lots of the agilawood materials saved, but also an agilawood essential oil product with a high added value is created; export foreign exchange earnings are expanded; and the method has considerable economic benefit and social benefit.

Description

Utilize the flora fermentation to promote the method that linaloe oil extracts

Technical field

The present invention relates to biological technical field; A kind of process method of utilizing multiple advantage fungi in the agalloch eaglewood endophyte or flora and agalloch eaglewood material to carry out mixed fermentation; Can significantly promote the further conversion of agalloch eaglewood material by this method, thereby increase substantially the extraction yield of linaloe oil.

Background technology

Homemade agalloch eaglewood is the pathology timber that thymelaeceae eaglewood Lignum Aquilariae Resinatum Aquilaria sinensis (Lour.) Gilg contains black resin, can produce through modes such as nature Edgeworthia chrysantha or artificial Edgeworthia chrysanthas.In China, Lignum Aquilariae Resinatum is unique plant resources of producing homemade agalloch eaglewood, is the distinctive precious medicinal plant of China.The linaloe oil that from agalloch eaglewood, extracts is again the agalloch eaglewood product of high added value, and along with the medicinal of linaloe oil and as the significantly increase of beauty and health care consumption, market is the trend that supply falls short of demand to the demand of linaloe oil series product.At present the first-class linaloe oil price in domestic and international market up to 300 yuan/gram, in great demand especially on middle east market, the foreign exchange earning prospect is very wide.

Because the fat content in the common agalloch eaglewood material is very low, extraction yield is merely about 0.23% usually, and very precious so output is few, therefore the domestic and international market is that supply falls short of demand always, and the agalloch eaglewood cost of material is also rising steadily at present; Become very urgent subject through the extraction yield of raising linaloe oil and the economic benefit of product.

In order to alleviate the imbalance between supply and demand in market; Improve the output and the economic benefit of linaloe oil; According to the fragrant rule of natural agalloch eaglewood natural birth with form mechanism, from the wild agalloch eaglewood that is growing, extract some agalloch eaglewood endogenetic fungus that can promote that agalloch eaglewood generates, ferment with the agalloch eaglewood material mixing through the purified special bacterial colony preparation of cultivating into of optimum combination advantage aroma former strain; Experiment shows the extraction yield that can promote and improve linaloe oil significantly; Extraction yield brings up to 0.48% from original 0.23%, and its output transforms through the flora preparation fermentation and is doubled manyly, gropes and test out operational path and the method that significantly improves linaloe oil output thus.

Summary of the invention

According to the invention on the basis that does not increase the agalloch eaglewood raw material supply, significantly improve the unit output of linaloe oil, utilize the agalloch eaglewood endophyte that the agalloch eaglewood material is carried out mixed fermentation for this reason and promote conversion processing, thereby significantly promote the yield of linaloe oil,

Summary of the invention is; In the natural agalloch eaglewood that is growing, extract many strains and have the fungi that advantage is produced fragrant ability; Coloured two spore bacterium (Diplodia sp.); Diplodia natalensis (Botryosphaeria rhodina); Sickle-like bacteria (fusarium) branch top spore mould (acreonium), light are deceived shell bacterial strains such as (fleischhakii) and are original seed through sterilization respectively, purifying cultivation, and its process is following;

The solid test tube strains that the agalloch eaglewood endophyte extracts, purifying is cultivated → combination liquid shaking table is cultivated the liquid spawn of flora bacterial classification → flora seed expansion and is cultivated through shaking table.

Embodiment

To from the many strains live body agalloch eaglewood endophyte the agalloch eaglewood plantation, Longnan, Qing Wan town, Beiliu City, Guangxi, extract the multiple fungi that produces fragrant advantageous ability that has respectively; Coloured two spore bacterium (Diplodia sp.), Diplodia natalensis (Botryosphaeria rhodina), sickle-like bacteria (fusarium) branch top spore mould (acreonium), light is deceived shell (fleischhakii)

Above-mentioned bacterial strains through extract respectively, sterilize and be inoculated in the seed culture medium of wort solid agar (glucose 10g/L, an albumen 10g/L, yeast extract 5g/L, NaCl10g/L, agar 20g/L pH7.0) puts 28 ℃ and cultivates and became the test tube original seed in 7 days.

Cutting youngster in the test tube plants the original seed fungi and inserts 100ml liquid nutrient medium (glucose 20g/L, an albumen 10g/L, yeast powder 5g/L respectively; Carnis Bovis seu Bubali cream 10g/L, sodium acetate 2g/L, lemon wild jujube g/L; SODIUMNITRATE 2mLpH6.5) triangular flask of 100ml * 10 is cultivated 200～250 rev/mins of rotating speeds 32 ℃ of combinations; Shaking table was cultivated 7 days, got the composite bacteria nutrient solution.

Get composite bacteria nutrient solution 500ml; Insert 10000ml in the liquid spawn culture medium that enlarges, (identical with the aforesaid liquid medium component, it is measured according to the corresponding increase of 10000ml; 28～32 ℃ of fermentation culture of room temperature; 200～250 rev/mins, shaking table was cultivated 5 days, composite bacteria or nutrient solution after obtaining enlarging.

Fermentative prepn with the agalloch eaglewood material; 200 kilograms of agalloch eaglewood siccatives; Pulverize to chip, insert fermentor tank, add flora nutrient solution 1000ml; (with 150 kilograms sterilized water dilution) permeate agent Tween-60 adds 200 gram mixings; The humidity of compound is pinched agglomerating rare spilling water with hand and is got final product, and 32～37 ℃ of fermentation reactions of temperature 15 days promote that fully the whitewood's composition in the agalloch eaglewood material obtains effecting reaction and conversion.

Agalloch eaglewood material after the conversion is again through the solvent extraction linaloe oil; Its extraction yield and former unleavened agalloch eaglewood material extraction contrast are improved largely, thereby on the basis that does not increase the agalloch eaglewood raw material supply, the yield of linaloe oil brings up to 0.48% from average 0.23%; Not only saved a large amount of agalloch eaglewood materials; And significantly increase production and high added value linaloe oil product, enlarged foreign exchange earning, have very considerable economic and social benefit.

Claims

1. utilize the flora fermentation to promote the method that linaloe oil extracts; Its inventive features is meant it can is to utilize single bacterial strain, also can be the flora that several kinds to tens kinds strain combinations are cultivated, through with the method for agalloch eaglewood material combined ferment, be used for significantly promoting and improving the extraction yield of linaloe oil.

2. utilize the flora fermentation to promote that four kinds of strain characteristicses of the method that linaloe oil extracts are sufficient; In the natural agalloch eaglewood that is growing, extract the multiple fungi that produces fragrant ability that has; Coloured two spore bacterium (Diplodia sp.), Diplodia natalensis (Botryosphaeria rhodina), sickle-like bacteria (fusarium) branch top spore mould (acreonium), light are deceived shell bacterial strains such as (fleischhakii) and are original seed through sterilization respectively, purifying cultivation.

3. several kinds of original seed fungies that cut in the test tube are inserted 100ml liquid nutrient medium (glucose 20g/L, an albumen 10g/L, yeast powder 5g/L respectively; Carnis Bovis seu Bubali cream 10g/L, sodium acetate 2g/L, lemon wild jujube g/L; SODIUMNITRATE 2mLpH6.5) triangular flask of 100ml * 10 is cultivated 200～250 rev/mins of rotating speeds 32 ℃ of combinations; Shaking table was cultivated 7 days, got the composite bacteria nutrient solution.

4. get composite bacteria nutrient solution 500ml; Insert 10000ml in the liquid spawn culture medium that enlarges, (identical with the aforesaid liquid medium component, it is measured according to the corresponding increase of 10000ml; 28～32 ℃ of fermentation culture of room temperature; 200～250 rev/mins, shaking table was cultivated 5 days, composite bacteria or nutrient solution after obtaining enlarging.

5. the fermentative prepn of agalloch eaglewood material; 200 kilograms of agalloch eaglewood siccatives; Pulverize to chip, insert fermentor tank, add flora nutrient solution 1000ml; (with 150 kilograms sterilized water dilution) permeate agent Tween-60 adds 200 gram mixings; The humidity of compound is pinched agglomerating not loosing with hand and is got final product, 32～37 ℃ of fermentation reactions of temperature 15 days, and the whitewood's composition in the promotion agalloch eaglewood material is fully reacted and is transformed.