CN102657136A - Indoor mass breeding method of edible fungus and mite in glove box - Google Patents
Indoor mass breeding method of edible fungus and mite in glove box Download PDFInfo
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- CN102657136A CN102657136A CN2012100134882A CN201210013488A CN102657136A CN 102657136 A CN102657136 A CN 102657136A CN 2012100134882 A CN2012100134882 A CN 2012100134882A CN 201210013488 A CN201210013488 A CN 201210013488A CN 102657136 A CN102657136 A CN 102657136A
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Abstract
The invention relates to an indoor mass breeding method of edible fungus and mite in a glove box, and belongs to the technical field of animal feeding. The glove box is characterized in that a ventilating and humidification control box is hung to the upper portion of the left side of a main box body, a diaphragm booster pump is disposed in the control box, a fan and a material access hole are reserved on the right side of the main box body, a seal door is arranged at the material access hole, an air inlet is reserved on the lower left of the main box body, an air outlet is reserved inside the fan on the upper right, a 400-mesh inset net is disposed in each of the air inlet and the air outlet, and a door cover with a sucker is mounted on each of the air inlet and the air outlet. An atomizing and humidifying nozzle is reserved at the central top of the interior of the main box body. A glove operation hole is located in front of the main box body. The mite can be bred in the indoor closed environment by the method, macro fungi (artificially cultivated edible fungi) can be cultivated to obtain mass mites equally developing by the method, and multiple experimental requirements can be met.
Description
One, technical field:
The present invention relates to a kind of method of utilizing the indoor mass rearing edible mushroom of glove box acarid; Comprise use, acarid indoor feeding, source---the cultivation of edible mushroom of obligate food of airtight glove box; Utilize the method can in indoor airtight environment, raise acarid and cultivation edible mushroom simultaneously, the use of operation gloves can be avoided the injury of acarid to human body.
Two, background technology
Edible fungus damage mite from young mite, if mite to the developmental process that becomes mite, all desirable food hypha of edible fungus, mushroom flower bud, fruit body, the propagation disease that often carries the germs of a disease is one of primary pest that causes the in poor harvest and even failure of Edible Fungi.Harm edible mushroom acarid of a great variety, 10 sections are arranged, as in southern mushroom-cultivating district with have a liking for wooden mite (
CaloglyphusSp.), a harmful long mite (
DolichocybeSp.), Lan Shi Boulogne mite (
Brennandania lambiKrozal) be main, Jiangsu one band then with the tyrophagus putrescentiae of Tyroglyphidae (
Tyrophagus putrescentiaeSchrank) be main.In addition, on woodear and Uricularia polytricha, endangering serious auricularia auriculajudae Lu Xi mite in recent years is U.S.'s report
Tarsonemus flovculus, Britain
TarsonemusmyceliophogusHussey,
Linopodes antennaepesBanks.
Present many research units carry out the research of biological property and non-environmental pollution control method to acarid, and the insecticidal power of new biologic product, anti-repelling properties and the test of food refusal property, and the screening of low toxicity, less-persistent pesticide and evaluation of pesticide effectiveness etc. all need a large amount of worm sources.But 2 kinds or 3 kinds of harmful mites mixing generations sometimes in the field, the same a kind of acarid difficulty that gather a large amount of growth unanimities from the field is very big, therefore need the artificial feeding in the laboratory.
To the raising of spider mite kind with test adopts sponge water pond methods, but this method is big for need mite amount, carry out multiple different tests research some deficiency that just seems.And edible mushroom itself is a fungi, and it is different that condition of culture and other plant have much, and at first culture matrix will be through sterilization treatment, and the environmental condition that vegetative stage and fruiting stage require is also different, and mycelial growth does not need illumination, the lucifuge cultivation, temperature 20-25 ℃.The fruiting stage requires that oxygen is sufficient, humidity 85% or more, only need scattered light, temperature different cultivars difference, and diversity ratio is bigger.If raise with blade as spider mite kind, be easy to rot after the high collection of fruit body of edible fungi water content, substitute feed again, therefore raising the edible mushroom acarid need cultivate edible mushroom simultaneously.In order to prevent acarid diffusion harm, need raise at airtight environment, so never be suitable for raising the raising method of edible mushroom acarid.The purpose of this invention is to provide a kind of method that is applicable to the edible fungus damage mite of indoor feeding, the method has designed a seal box.But ventilation in the glove box, humidification can satisfy edible fungi growth and grow needs; Ventilating opening adopts 400 order fly nets, can effectively intercept acarid, prevents diffusion; In addition,, do not open and to carry out various experiments to acarid, can pass in and out bacterium bag and sampling when opening owing to be provided with gathering hole in the side.
Three, summary of the invention
Technical problem
Technical problem to be solved by this invention provides a kind of method of indoor mass rearing edible mushroom acarid, and this method realizes that through a kind of gloves case this raising method has advantages such as easy, practical, efficient.
Technical scheme
A kind of method of utilizing glove box indoor feeding edible mushroom acarid of the present invention; Said glove box is: the casing material of main box (1) is a polymethyl methacrylate; Control panel, diaphragm booster pump (3) are arranged in plug-in ventilation of casing left upper portion and humidification control cabinet (2), the control cabinet (2); Main box (1) right side is provided with blower fan (9), material import and export (12), and material import and export is sealed up a door (13), and air inlet (5) is positioned at the main box lower left; Air outlet (10) is positioned at upper right side blower fan (9), and the door closure of sucker property all is installed on the built-in 400 order fly nets in air port (4 and 8), air inlet, the air outlet;
Directly in the main box atomizing humidifying performance nozzle (7) is arranged, (11) 2 of gloves gathering holes are positioned at the main box dead ahead;
The size of said glove box main box (1) is long 800 mm * wide 500mm * high 400mm; The control cabinet of humidification and ventilation (2) is positioned at outer top, casing left side; Size: long 200mm * wide 200mm * high 100mm, assembling 220v power switch and humidification button, ventilation button on the control panel, diaphragm booster pump (3) is installed in control cabinet (2) casing; Link to each other with humidification button on the control panel; Be provided with inlet tube and outlet tube, water inlet pipe connects the water source, and high-pressure outlet (6) connects atomizing humidifying performance nozzle (7); The atomizing humidifying performance nozzle is installed in main box (1) inner top center; Be connected to diaphragm booster pump (3) delivery port through high-pressure outlet (6); Spray angle 80-90 °, droplet 20-40 μ m, spraying is no dead angle evenly; Hypha of edible fungus and fruit body are not had the machinery injury, reduce the probability of living contaminants;
Air inlet (5) be installed in main box (1) left side apart from the base 100mm, apart from operating surface 100mm; Air outlet (10) is installed in main box (1) three o'clock wind machine enclosure (9) outside; High 40 mm, aperture 75 mm, built-in 400 order fly nets (4,8) can effectively intercept acarid, prevent flight; The door closure of sucker property all is installed on air inlet (5), the air outlet (10), operate simpler, door closure diameter 110 mm, the time spent does not cover door closure, is exactly a closed environment in the case; The direct current turbofan is installed in the blower fan cabinet (9), and the ventilation button links to each other on direct current turbofan and the control panel, blower fan cabinet (9) be installed in main box (1) right side far from the base 220mm place;
Gloves gathering hole (11): high 40mm, diameter 160mm, 2 gathering hole spacing 350mm, centre-to-centre spacing base 130mm adopts thickening, 750mm overlength emgloves;
Material import and export (12): high 30mm, diameter 160mm, be positioned at casing 1 right side far from the base 170mm, apart from operating surface 150mm place; Material import and export (13) diameter 190 mm that seal up a door, the sealing of silicon rubber O type circle, bolt is screwed.
Said diaphragm booster pump (3) adopts Pulan enlightening PLD-1205 type membrane pump
; The atomizing humidifying performance nozzle is that Nanjing corner stone electronics technology Co., Ltd produces; The direct current turbofan is the strange grand AVC BA12032B12M direct current turbofan of 12V, realizes the glove box inner air flow.
Described method comprises:
1) cultivation in acarid food source: the glove box casing is placed edible mushroom bottle or fruiting bag, cultivates edible mushroom flat mushroom, tea tree mushroom, Xingbao mushroom, Uricularia polytricha, woodear, mushroom or elegant precious mushroom etc.;
2) a large amount of artificial feedings of acarid: after edible mushroom sends out bacterium good, insert the female mite of single maturation in each fruiting bottle or the fruiting bag, capped or tighten sack;
It is 18-25 ℃ that air-conditioning is regulated indoor temperature, and glove box ventilates 2 times every day, and 30min ventilates at every turn; Ventilate and finish, air inlet (5), air outlet (10) door closure are set up; Press the humidification button again, humidification 3 minutes during humidification, will confirm that at first water pipe is under water, and water will clean free from admixture, and humidity remains on 60%, and every day, humidification was twice.Promptly obtain the consistent same acarid of a large amount of growths about 40 days.
With growing consistent same acarid in a large number, subculture is raised continuously, all can obtain the population of a large amount of same a kind of acarids throughout the year.
Beneficial effect
The gloves case simplicity of design that the present invention utilizes, workable, safety durable, flexible operation, the use of operation gloves can prevent the injury to the keeper such as acarid, and is capable of washing, recycle.
Whole glove box space is spacious, can satisfy the needs that grow of each plant species, macro fungi, but the successive propagation acarid obtains the sample of indoor high experimental population density.
The method can be raised acarid in indoor airtight environment, can utilize the edible mushroom of cultivation to be grown consistent acarid in a large number again, satisfies multiple test requirements document.
Four, description of drawings
A kind of glove box front view that relates in the method for glove box indoor feeding edible mushroom acarid that utilizes of Fig. 1
Annotate: 800 mm (length) x500 mm (wide) x400 mm (height); Casing material: polymethyl methacrylate (lucite); The high 40mm of gathering hole, R=80mm;
A kind of glove box left view that relates in the method for glove box indoor feeding edible mushroom acarid that utilizes of Fig. 2
Annotate: control cabinet size: 200mm (length) * 200mm (wide) * 100mm (height); The high 40mm of air inlet, internal diameter R=37.5mm, external diameter R=55mm, built-in 400 mesh filter screens.
A kind of glove box right view that relates in the method for glove box indoor feeding edible mushroom acarid that utilizes of Fig. 3
Annotate: high 140 mm of air outlet, internal diameter R=37.5 mm, external diameter R=55mm, filter screen 400 orders; The high 130mm of material import and export, internal diameter R=80mm,, external diameter R=95mm.
Among the figure 1, main box 2, humidification, ventilation control cabinet 3, diaphragm booster pump 4, air inlet fly net 5, air inlet 6, high-pressure outlet 7, atomizing humidifying performance nozzle 8, air outlet fly net 9, blower fan cabinet 10, air outlet 11, gloves gathering hole 12, material import and export 13, material import and export seal up a door
Five, embodiment
Below in conjunction with accompanying drawing the present invention is done further description.
1) acarid raising method of the present invention is to utilize a kind of novel glove box to realize this glove box
Belong to seal box, form by main box and control cabinet two parts:
The size of main box (1) is 800 (length) mm * 500 (wide) mm * 400 (height) mm, and material is polymethyl methacrylate (lucite), is furnished with to ventilate and damping device;
Humidification is positioned at the casing left side with the control cabinet (2) that ventilation is ventilated; Size: 200 (length) mm * 200 (wide) mm * 100 (height) mm; Control cabinet (2) inner control panel-mounted 220v power switch with, diaphragm booster pump (3) is housed in the control cabinet (2); Diaphragm booster pump adopts Pulan enlightening PLD-1205 type membrane pump, links to each other with the humidification button, is provided with inlet tube and outlet tube, and water inlet pipe connects the water source, and high-pressure outlet (6) connects atomizing humidifying performance nozzle (7); Atomizing humidifying performance nozzle (Nanjing corner stone electronics technology Co., Ltd) is installed in main box (1) inner top center, is connected to diaphragm booster pump (3) delivery port, spray angle 80-90 ° through high-pressure outlet (6); Droplet 20-40 μ m, spraying is no dead angle evenly, and hypha of edible fungus and fruit body are not had the machinery injury; Reduce the probability of living contaminants; Can realize that humidification is even in the case, host plant not had the machinery injury, and reach humidity 85%.
2) cultivation in acarid food source.The casing of glove box is provided with and ventilates and the humidification control box, ventilation and humidification in the controlled casing.The high 400mm of main box (2) can satisfy the requirement of growing of most kinds such as flat mushroom, tea tree mushroom, Xingbao mushroom, Uricularia polytricha, woodear, mushroom, elegant precious mushroom.The interior space of main box (2) is spacious, can put simultaneously 30 edible mushroom bottles (volume: 85-1000ml) or the fruiting bag (specification: 170mm * 330mm), be the ecotope of acarid growth can do the ecological population experiment of acarid.
3) a large amount of artificial feedings of acarid.Air inlet (5) and air outlet (10) be installed in respectively main box (1) left side apart from the base 100mm, apart from operating surface 100mm and main box (1) three o'clock wind machine enclosure (9) outside; High 40 mm, aperture 75 mm, built-in 400 order fly nets (4,8) can effectively intercept acarid, prevent flight; The door closure of sucker property all is installed on air inlet (5), the air outlet (10), operate simpler, door closure diameter 110 mm, the time spent does not cover door closure, is exactly a closed environment in the case;
Be installed in the blower fan cabinet (9) main box (1) right side far from the base 220mm place; The direct current turbofan is installed in the blower fan cabinet (9), and (strange grand AVC BA12032B12M 12V) links to each other with the ventilation button, realizes the glove box inner air flow; Power is little; Noise is low, and wind speed slow (75CFM) can prevent that meiofauna such as acarid are flowing in the case with air to drift about;
The built-in 400 order fly nets of inlet and outlet prevent meiofauna---acarid is invaded outward; The door closure of ability sucker property all is installed on air inlet, the air outlet, covers door closure when stuffy, closed environment in the casing.
Gloves gathering hole (11): high 40mm; Diameter 160mm; Two gathering hole spacing 350mm, centre-to-centre spacing base 130mm adopts thickening; 750mm overlength emgloves (Nanjing corner stone electronics technology Co., Ltd) has very outstanding repellence to ozone aging, oxygen, weathering, heating and chemicals;
Material import and export (12): high 30mm, diameter 160mm.Be positioned at casing 1 right side far from the base 170mm, apart from operating surface 150mm place; Material import and export (13) diameter 190 mm that seal up a door, the sealing of silicon rubber O type circle, bolt is screwed.
This glove box simplicity of design is practical, and the little noise of power is low, environmental protection and energy saving.Can solve the same acarid efficient of indoor feeding low, individual little, do not stop changing drawback such as foodstuff.Concrete raising method is following:
At first, make edible mushroom bag/bottle.Suitable prescription according to required edible fungus variety is ready to composts or fertilisers of cultivating, and material is 1:1.2 with the ratio of water, the back charging that stirs, if use the fruiting bag, material is selected polypropylene knuckle bag for use, and size is: 170 * 350 ~ 380mm; As use the fruiting bottle, and select capacity 800-1000ml, bore 58-70mm, the anti-white translucent polypropylene plastics bottle of high temperature more than 130 ℃ for use, bottle cap adopts has cotton lid maybe can satisfy non-cotton cover ventilative and the bacteriological filtration requirement.About every packed siccative 0.5 kg, available automatic packer also can pack by hand, and material is high at 150-180mm.High pressure steam sterilization is adopted in the sterilization in time of charging back, and the autoclave internal pressure is stabilized in 0.15MPa, keeps 2.5h.Sterilization finishes, natural step-down.
In super-clean bench, insert edible fungus species, after the inoculation, lucifuge is cultivated in incubator, requires: temperature 20-24 ℃, and relative air humidity about 65%.Cultivate 20d-25d, treat that mycelia covers with bacterium bag/bottle.Promptly can be used for acarid cultures.
Then, the acarid that collects is taken to indoor, open sealing up a door of material import and export; Fruiting bag/bottle, the acarid sample of sending out bacterium good are put into glove box; Cover and seal up a door, operating personnel are with gloves, get single female one-tenth mite and forward in fruiting bag/bottle; On label, indicate acarid kind, source, date, on the fruiting bag/bottle of registering.Put into a Hygrothermograph in the case.According to the temp. and humidity of its demonstration, environment in the control cabinet.Open left side control cabinet power supply, pressing the ventilation button can ventilate, and during ventilation, the door closure of inlet and outlet will be taken away, and ventilates every day 2 times, and 30min ventilates at every turn; Ventilate and finish, door closure is set up, press the humidification button again, during humidification; Will confirm that at first water pipe is under water, water will clean free from admixture, and the acarid desired moisture level is 60%; Edible mushroom requires more than 85%, adopts each humidification 3 minutes that only need of high atomization humidifying spraying nozzle, and every day, humidification was twice; Press the humidification button and get final product humidification, click again and close.The adjusting of temperature is realized that by air-conditioning indoor temperature keeps 18-25 ℃.Can be grown consistent same acarid about 40 days in a large number, subculture is raised continuously, and the egg laying amount of female one-tenth mite does not obviously reduce, and can obtain the population of a large amount of same a kind of acarids throughout the year.
Claims (4)
1. method of utilizing the indoor mass rearing edible mushroom of glove box acarid; It is characterized in that; Said glove box is: the casing material of main box (1) is a polymethyl methacrylate; Control panel, diaphragm booster pump (3) are arranged in plug-in ventilation of casing left upper portion and humidification control cabinet (2), the control cabinet (2); Main box (1) right side is provided with blower fan (9), material import and export (12), and material import and export is sealed up a door (13), and air inlet (5) is positioned at the main box lower left; Air outlet (10) is positioned at upper right side blower fan (9), all be equipped with on the built-in 400 order fly nets in air port (4 and 8), air inlet, the air outlet can sucker property door closure;
Directly in the main box atomizing humidifying performance nozzle (7) is arranged, (11) 2 of gloves gathering holes are positioned at the main box dead ahead;
The size of said glove box main box (1) is long 800 mm * wide 500mm * high 400mm; The control cabinet of humidification and ventilation (2) is positioned at outer top, casing left side; Size: long 200mm * wide 200mm * high 100mm, assembling 220v power switch and humidification, ventilation button on the control panel, diaphragm booster pump (3) is installed in control cabinet (2) casing; Link to each other with the humidification button; Be provided with inlet tube and outlet tube, water inlet pipe connects the water source, and high-pressure outlet (6) connects atomizing humidifying performance nozzle (7); The atomizing humidifying performance nozzle is installed in main box (1) inner top center, is connected to diaphragm booster pump (3) delivery port through high-pressure outlet (6), and spray angle 80-90 °, droplet 20-40 μ m;
Air inlet (5) be installed in main box (1) left side apart from the base 100mm, apart from operating surface 100mm; Air outlet (10) is installed in main box (1) three o'clock wind machine enclosure (9) outside; High 40 mm, aperture 75 mm, built-in 400 order fly nets (4,8) can effectively intercept acarid, prevent flight; The door closure of sucker property all is installed on air inlet (5), the air outlet (10), operate simpler, door closure diameter 110 mm, the time spent does not cover door closure, is exactly a closed environment in the case; The direct current turbofan is installed in the blower fan cabinet (9), and the ventilation button links to each other on direct current turbofan and the control panel, blower fan cabinet (9) be installed in main box (1) right side far from the base 220mm place;
Gloves gathering hole (11): high 40mm, diameter 160mm, 2 gathering hole spacing 350mm, centre-to-centre spacing base 130mm adopts thickening, 750mm overlength emgloves;
Material import and export (12): high 30mm, diameter 160mm, be positioned at casing 1 right side far from the base 170mm, apart from operating surface 150mm place; Material import and export (13) diameter 190 mm that seal up a door, the sealing of silicon rubber O type circle, bolt is screwed.
2. method according to claim 1; It is characterized in that; Said diaphragm booster pump (3) adopts Pulan enlightening PLD-1205 type membrane pump
; The atomizing humidifying performance nozzle is that Nanjing corner stone electronics technology Co., Ltd produces; The strange grand AVC BA12032B12M direct current turbofan of 12V is installed in the blower fan cabinet (9), is realized the glove box inner air flow.
3. method according to claim 1 and 2 is characterized in that,
1) cultivation in acarid food source: place edible mushroom bottle or fruiting bag in the glove box casing, can cultivate edible mushroom flat mushroom, tea tree mushroom, Xingbao mushroom, Uricularia polytricha, woodear, mushroom or elegant precious mushroom;
2) a large amount of artificial feedings of acarid: after edible mushroom sends out bacterium good, insert the female mite of single maturation in each fruiting bottle or the fruiting bag, capped or tighten sack;
It is 18-25 ℃ that air-conditioning is regulated indoor temperature, before ventilating door closure is opened, and ventilates humidification 2 times every day 2 times; Each ventilation 30min ventilates and finishes, and air inlet (5), air outlet (10) magnetic door closure are set up, and presses humidification button humidification again 3 minutes, promptly obtains the consistent same acarid of a large amount of growths about 40 days.
4. method according to claim 3 is characterized in that, with growing consistent same acarid in a large number, subculture is raised continuously, obtains the population of a large amount of same a kind of acarids.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2012100134882A CN102657136A (en) | 2012-01-17 | 2012-01-17 | Indoor mass breeding method of edible fungus and mite in glove box |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2012100134882A CN102657136A (en) | 2012-01-17 | 2012-01-17 | Indoor mass breeding method of edible fungus and mite in glove box |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105831023A (en) * | 2016-06-14 | 2016-08-10 | 福建省农业科学院食用菌研究所 | Cultivating method of dolichocybidae |
| CN107047488A (en) * | 2017-05-11 | 2017-08-18 | 中华人民共和国无锡出入境检验检疫局 | A kind of bio-safety feeding facility for quarantine procedures |
| CN112005969A (en) * | 2019-05-30 | 2020-12-01 | 杭州博源有害生物防制有限公司 | Cockroach raising box |
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| JPS51135283A (en) * | 1976-04-16 | 1976-11-24 | Reiichi Okada | Experimental breeder |
| CN2538132Y (en) * | 2002-04-18 | 2003-03-05 | 董礼创 | Gas regenerating regulator for edible mushroom chamber |
| KR20050104479A (en) * | 2004-04-29 | 2005-11-03 | 양남웅 | Glove box type incubator |
| CN201369984Y (en) * | 2009-03-24 | 2009-12-30 | 张大维 | Combined type isolator with automatic temperature control |
| CN202061628U (en) * | 2011-04-18 | 2011-12-07 | 浙江工业职业技术学院 | Glove box type biosafety cabinet |
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| JPS51135283A (en) * | 1976-04-16 | 1976-11-24 | Reiichi Okada | Experimental breeder |
| CN2538132Y (en) * | 2002-04-18 | 2003-03-05 | 董礼创 | Gas regenerating regulator for edible mushroom chamber |
| KR20050104479A (en) * | 2004-04-29 | 2005-11-03 | 양남웅 | Glove box type incubator |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105831023A (en) * | 2016-06-14 | 2016-08-10 | 福建省农业科学院食用菌研究所 | Cultivating method of dolichocybidae |
| CN107047488A (en) * | 2017-05-11 | 2017-08-18 | 中华人民共和国无锡出入境检验检疫局 | A kind of bio-safety feeding facility for quarantine procedures |
| CN112005969A (en) * | 2019-05-30 | 2020-12-01 | 杭州博源有害生物防制有限公司 | Cockroach raising box |
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Application publication date: 20120912 |