CN102618532A - 基于磁珠法从植物叶片中提取基因组dna的试剂盒及其提取方法 - Google Patents
基于磁珠法从植物叶片中提取基因组dna的试剂盒及其提取方法 Download PDFInfo
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Cited By (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103898092A (zh) * | 2014-01-27 | 2014-07-02 | 西安天隆科技有限公司 | 一种快速磁珠法提取植物组织基因组dna的试剂盒及方法 |
CN104630208A (zh) * | 2015-02-11 | 2015-05-20 | 杭州百迈生物技术有限公司 | 提取基因组dna的试剂盒和提取方法 |
CN106119394A (zh) * | 2016-08-26 | 2016-11-16 | 刘明涛 | 一种世纬苣苔的核苷酸序列鉴定方法及核苷酸序列的应用 |
CN106480014A (zh) * | 2015-09-02 | 2017-03-08 | 创想生物科技有限公司 | 选择性分离核酸的方法及其套组 |
CN107267502A (zh) * | 2017-08-10 | 2017-10-20 | 广东省生物工程研究所(广州甘蔗糖业研究所) | 一种高效提取甘蔗糖制品dna的试剂盒和方法 |
CN109055363A (zh) * | 2018-09-21 | 2018-12-21 | 武汉菲沙基因信息有限公司 | 一种适用于筛选超长片段核酸的磁珠试剂及筛选方法 |
CN110358762A (zh) * | 2019-07-26 | 2019-10-22 | 云南省农业科学院热区生态农业研究所 | 一种提取植物叶片基因组dna的试剂盒及其提取方法 |
CN111197073A (zh) * | 2019-12-19 | 2020-05-26 | 武汉艾米森生命科技有限公司 | 从粪便中提取dna样本的方法和结直肠癌相关基因的甲基化检测方法 |
CN112501155A (zh) * | 2020-11-06 | 2021-03-16 | 南方医科大学顺德医院(佛山市顺德区第一人民医院) | 一种应用于转基因小鼠基因鉴定的dna提取试剂盒 |
CN114015681A (zh) * | 2021-11-15 | 2022-02-08 | 深圳市人民医院 | 一种改进的多糖多酚植物ctab的提取方法 |
CN114426967A (zh) * | 2020-10-29 | 2022-05-03 | 西南大学 | 一种无毒的植物通用型高纯度基因组dna快速提取方法和试剂盒 |
CN115873848A (zh) * | 2022-12-24 | 2023-03-31 | 蓝景科信河北生物科技有限公司 | 植物dna提取试剂盒 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2000063362A1 (en) * | 1999-04-21 | 2000-10-26 | Annovis, Inc. | Magnetic dna extraction kit for plants |
CN102344921A (zh) * | 2011-10-17 | 2012-02-08 | 西安金磁纳米生物技术有限公司 | 一种用金磁微粒纯化植物组织基因组dna的方法 |
CN102344920A (zh) * | 2011-10-17 | 2012-02-08 | 西安金磁纳米生物技术有限公司 | 一种用金磁微粒纯化植物种子基因组dna的方法 |
-
2012
- 2012-05-02 CN CN2012101313235A patent/CN102618532A/zh active Pending
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2000063362A1 (en) * | 1999-04-21 | 2000-10-26 | Annovis, Inc. | Magnetic dna extraction kit for plants |
CN102344921A (zh) * | 2011-10-17 | 2012-02-08 | 西安金磁纳米生物技术有限公司 | 一种用金磁微粒纯化植物组织基因组dna的方法 |
CN102344920A (zh) * | 2011-10-17 | 2012-02-08 | 西安金磁纳米生物技术有限公司 | 一种用金磁微粒纯化植物种子基因组dna的方法 |
Non-Patent Citations (6)
Title |
---|
丛玉隆: "《临床实验室管理》", 31 August 2004, 中国医药科技出版社 * |
何春燕等: "《医学生物化学实验指导》", 30 September 2010, 湖北科学技术出版社 * |
刘松财: "《生物化学实验技术》", 30 June 2010, 吉林大学出版社 * |
史仁玖等: "药用植物泰山四叶参DNA提取方法的研究", 《时珍国医国药》 * |
许良中: "《实用肿瘤病理方法学》", 31 December 1997, 上海医科大学出版社 * |
闫桂琴: "《遗传学实验教程》", 31 December 2010, 科学出版社 * |
Cited By (17)
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CN103898092B (zh) * | 2014-01-27 | 2016-08-10 | 西安天隆科技有限公司 | 一种快速磁珠法提取植物组织基因组dna的试剂盒及方法 |
CN103898092A (zh) * | 2014-01-27 | 2014-07-02 | 西安天隆科技有限公司 | 一种快速磁珠法提取植物组织基因组dna的试剂盒及方法 |
CN104630208A (zh) * | 2015-02-11 | 2015-05-20 | 杭州百迈生物技术有限公司 | 提取基因组dna的试剂盒和提取方法 |
CN104630208B (zh) * | 2015-02-11 | 2018-01-02 | 杭州百迈生物股份有限公司 | 提取基因组dna的试剂盒和提取方法 |
CN108048449A (zh) * | 2015-02-11 | 2018-05-18 | 杭州百迈生物股份有限公司 | 一种提取基因组dna的试剂盒 |
CN108048449B (zh) * | 2015-02-11 | 2021-10-08 | 杭州百迈生物股份有限公司 | 一种提取基因组dna的试剂盒 |
CN106480014B (zh) * | 2015-09-02 | 2020-05-15 | 创想生物科技有限公司 | 选择性分离核酸的方法及其套组 |
CN106480014A (zh) * | 2015-09-02 | 2017-03-08 | 创想生物科技有限公司 | 选择性分离核酸的方法及其套组 |
CN106119394A (zh) * | 2016-08-26 | 2016-11-16 | 刘明涛 | 一种世纬苣苔的核苷酸序列鉴定方法及核苷酸序列的应用 |
CN107267502A (zh) * | 2017-08-10 | 2017-10-20 | 广东省生物工程研究所(广州甘蔗糖业研究所) | 一种高效提取甘蔗糖制品dna的试剂盒和方法 |
CN109055363A (zh) * | 2018-09-21 | 2018-12-21 | 武汉菲沙基因信息有限公司 | 一种适用于筛选超长片段核酸的磁珠试剂及筛选方法 |
CN110358762A (zh) * | 2019-07-26 | 2019-10-22 | 云南省农业科学院热区生态农业研究所 | 一种提取植物叶片基因组dna的试剂盒及其提取方法 |
CN111197073A (zh) * | 2019-12-19 | 2020-05-26 | 武汉艾米森生命科技有限公司 | 从粪便中提取dna样本的方法和结直肠癌相关基因的甲基化检测方法 |
CN114426967A (zh) * | 2020-10-29 | 2022-05-03 | 西南大学 | 一种无毒的植物通用型高纯度基因组dna快速提取方法和试剂盒 |
CN112501155A (zh) * | 2020-11-06 | 2021-03-16 | 南方医科大学顺德医院(佛山市顺德区第一人民医院) | 一种应用于转基因小鼠基因鉴定的dna提取试剂盒 |
CN114015681A (zh) * | 2021-11-15 | 2022-02-08 | 深圳市人民医院 | 一种改进的多糖多酚植物ctab的提取方法 |
CN115873848A (zh) * | 2022-12-24 | 2023-03-31 | 蓝景科信河北生物科技有限公司 | 植物dna提取试剂盒 |
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