CN102599112B

CN102599112B - Method for quickly collecting pupal parasitic wasps

Info

Publication number: CN102599112B
Application number: CN 201210053323
Authority: CN
Inventors: 张忠; 于爱莲; 任倩; 杜迎钢
Original assignee: Taishan Medical University
Current assignee: Taishan Medical University
Priority date: 2011-06-29
Filing date: 2012-03-03
Publication date: 2013-10-09
Anticipated expiration: 2032-03-03
Also published as: CN102599112A

Abstract

The invention discloses a method for quickly collecting pupal parasitic wasps, which uses a mesh bag for spraying mixture of host pupa homogenate solution and hyromel to trap newly emerging parasitic wasps. Compared with artificial collection by suction pipes and induced collection purely by lamplight, the method is simple and quick. Injury caused by mechanical damage in blowing and suction can be reduced, and the survival time of adult parasitic wasps can be prolonged.

Description

A kind of pupa time parasitic wasp fast collecting method

Technical field

The invention belongs to biotechnology, relate to the fast collecting method of parasitic wasp in a kind of pupa time.

Background technology

Parasitic wasp is the important natural enemy of insect, parasitic wasp breed foundation with method for releasing on a large scale, significant to effectively utilizing of parasitic wasp.In reproductive process, relate to the collection of parasitic wasp, at present, two kinds of methods are mainly adopted in the collection of parasitic wasp: (1) light lures collection, but then efficient is not high for the not strong parasitic wasp of phototaxis; (2) manually utilize the pooter collecting method, waste time and energy, and more to the mechanical wounding of parasitic wasp, thereby how to set up the method for quick collection parasitic wasp, also be one of important technology difficult problem in the parasitic wasp reproductive process.

Summary of the invention

The present invention proposes the method for a kind of quick collection parasitic wasp in pupa time, mainly be to have proposed a kind ofly to lure collection with the mesh bag that sprayed host insect homogenate and hydromel mixture, overcome the deficiencies in the prior art, can improve the collection efficiency of parasitic wasp in pupa time greatly, reduce acquisition time.

For realizing above purpose, the present invention proposes following technical scheme:

A kind of pupa time parasitic wasp fast collecting method, it comprises the steps:

Step 1 with parasitism after, parasitic wasp host's pupa of soon sprouting wings is put in the Cans;

Step 2 is chosen mesh diameter and is made the mesh bag of an end opening less than the gauze of parasitic wasp build, and the mesh bag bore is a bit larger tham the can bottleneck, about 30 cm of mesh bag length, and the mesh bag mouth has bungee, and lanyard is arranged at the bottom;

Step 3 with the homogenate of host insect pupa after, be dissolved in phosphate buffer, after the filtration, add honey in 5% ratio, stir evenly, standby;

The liquid that step 4 makes step 3 is sprayed on the mesh bag equably, with moistening but do not drip and be advisable, dries in the shade;

Step 5 tips upside down on the can bottleneck that fills by the parasitized host pupa with mesh bag, the tension bungee, and with the lanyard hang-up of mesh bag bottom, place under the light;

Be subjected to the attraction of host's homogenate and hydromel mixture and light after step 6 parasitic wasp sprouts wings, upwards fly or creep, fall within on the mesh bag, if parasitic wasp is abundant in the mesh bag, interchangeable another mesh bag is collected;

Step 7 concentrates on the parasitic wasp in the mesh bag, raises the hydromel with 20%, waits for discharging or expanding propagation.

Described host insect pupa is imported cabbageworm pupa or Sarcophga fuscicauda pupa.

A kind of pupa time parasitic wasp fast-collection device, its main body is the mesh bag made of gauze of end opening one by one, sprays the mixture that host insect pupa homogenate and hydromel are arranged on the mesh bag.

Pupa time of the present invention, the parasitic wasp fast collecting method can improve collection efficiency, reduced acquisition time, and reduced the mechanical wounding to parasitic wasp.

The present invention will be further described below in conjunction with embodiment, it is to be noted that following embodiment only is the explanation of the invention explanation, rather than restriction.

Example 1:

To spray the mesh bag of imported cabbageworm pupa homogenate and honey liquor mixture, place and fill by on the Cans of the parasitic imported cabbageworm pupa of crossing of chrysalis tiny golden wasp, collect the chrysalis tiny golden wasp adult after sprouting wings, 95% parasitic wasp adult can enter in the mesh bag in 10 minutes, and only collect with the light method for enticing collection, after 40 minutes, 20% the parasitic wasp of still having an appointment rests in the Cans.Artificially collect with pooter, approximately need 120 minutes, after 1 day, the lethality of parasitic wasp adult but reaches 15%, lures the lethality of integrated worm less than 5%.

Example 2:

To spray the mesh bag of Sarcophga fuscicauda pupa homogenate and honey liquor mixture, place and fill by on the Cans of the parasitic Sarcophga fuscicauda pupa of crossing of calliphorid pupa collection tiny golden wasp, collect the calliphorid pupa collection tiny golden wasp adult after sprouting wings, 80% parasitic wasp adult can enter in the mesh bag in 15 minutes, and only collect with the light method for enticing collection, after 120 minutes, 50% the parasitic wasp of still having an appointment rests in the Cans.Collect with pooter, approximately need 180 minutes, after 1 day, the lethality of parasitic wasp adult but reaches 25%, lures the lethality of collection less than 5%.And when collecting with pooter, need stand the bad smell of fly pupa and the fly puparium that mistake sucks, the harm operator's is healthy.

Claims

One kind pupa time parasitic wasp fast collecting method, it comprises the steps:

Step 1 with parasitism after, parasitic wasp host's pupa of soon sprouting wings is put in the Cans;

Step 2 is chosen mesh diameter and is made the mesh bag of an end opening less than the gauze of parasitic wasp build, and the mesh bag bore is a bit larger tham the can bottleneck, about 30 cm of mesh bag length, and the mesh bag mouth has bungee, and lanyard is arranged at the bottom;

Step 3 with the homogenate of host insect pupa after, be dissolved in phosphate buffer, after the filtration, add honey in 5% ratio, stir evenly, standby;

The liquid that step 4 makes step 3 is sprayed on the mesh bag equably, with moistening but do not drip and be advisable, dries in the shade;

Step 5 tips upside down on the can bottleneck that fills by the parasitized host pupa with mesh bag, the tension bungee, and with the lanyard hang-up of mesh bag bottom, place under the light;

Be subjected to the attraction of host's homogenate and hydromel mixture and light after step 6 parasitic wasp sprouts wings, upwards fly or creep, fall within on the mesh bag, if parasitic wasp is abundant in the mesh bag, interchangeable another mesh bag is collected;

Step 7 concentrates on the parasitic wasp in the mesh bag, raises the hydromel with 20%, waits for discharging or expanding propagation.
A kind of pupa time according to claim 1 parasitic wasp fast collecting method, it is characterized in that: described host insect pupa is imported cabbageworm pupa or Sarcophga fuscicauda pupa.