CN117136913A - Indoor propagation method for grassland caterpillar wasp - Google Patents
Indoor propagation method for grassland caterpillar wasp Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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Abstract
The invention belongs to the field of biotechnology application, and discloses an indoor propagation method for a meadow caterpillar wasp, which utilizes cabbage caterpillar pupae to breed the wasp, and comprises the following steps: 1) Raising cabbage caterpillar larvae until pupation, and then sterilizing and heating; 2) Inserting parasitic wasps into the treated cabbage caterpillar pupae which are obtained in the step 1) and serve as hosts, and carrying out parasitic wasps at room temperature under natural light; 3) Firstly, placing the parasitic pupa obtained in the step 2) at room temperature for 6-8 days, and then breaking diapause of the larvae; 4) Placing the pupae obtained in the step 3) at room temperature until the pupae emerges, and carrying out photoperiod L: d=16: 8, 8; obtaining the emerged golden bees. The diapause of the mature larvae of the trichogramma of part or all grasslands can be broken through the low-temperature treatment and the ecdysone treatment, so that the breeding for a plurality of generations in one year can be realized, and the population expansion scale can be improved.
Description
Technical Field
The invention belongs to the field of biotechnology application, and particularly relates to an indoor propagation method for a parasitic wasp population of grassland caterpillars.
Background
The grassland caterpillars are one kind of pests of Lepidoptera Podoptera grassland caterpillars, and are one of the main pests in the alpine grasslands of Qinghai-Tibet plateau in China. Grassland caterpillars are mainly various grasses of Cyperaceae, gramineae, leguminosae, polygonaceae and Rosaceae, and cause grass deficiency, which seriously affects the development of animal husbandry. In addition, grassland caterpillars have certain influence on human and livestock, and body hair and cocoon hair of the grassland caterpillars are extremely toxic, so that oral mucosa of cattle and sheep are red and swollen, and small bubbles, tooth root or tongue erosion and the like are generated. The prevention and control work of the grassland caterpillars is well done, the outbreak of the grassland caterpillars is restrained, the ecological stability of the grassland is ensured, and the development of animal husbandry is restrained.
At present, the measures for preventing and controlling the grassland caterpillars are mainly chemical prevention and control measures and are supplemented with a certain biological prevention and control measure. The biological control measures mainly depend on parasitic natural enemies and worm-derived microorganisms, for example, the cell test results show that the parasitic bees can be released to reduce the density of the trichogramma by 70.6% and the parasitic rate of the trichogramma can reach 76% when the parasitic natural enemies of the trichogramma in the period of releasing the chrysalis of the grasslands are released, and the good control effect is shown. The biological prevention and control of natural enemies such as parasitic wasps are used as a supplement and partial replacement means for chemical prevention and control of pests, so that the problems of pesticide residues, pest resistance, environmental pollution and the like caused by excessive use and abuse of chemical pesticides can be relieved, and sustainable green development of grassland industry is facilitated.
The grassland trichogramma is parasitic wasp of the family of the hymenoptera, and the parasitic rate can reach 33% when the parasitic wasp is parasitic dominant species of the grassland trichogramma in the pupal period in the Qinghai Jade tree area. Under natural conditions, the first generation of the grassland trichogramma occurs in one year, the adult bee activity time is 7 middle ten days to 8 late ten days, after the parasitic wasp lays eggs in the grassland trichogramma pupa, the eggs develop to the mature larva after 10-15 days, diapause and overwintering are carried out, and the diapause period is about 300 days. In recent years, along with the increasing emphasis of the caterpillars of the grasslands, the demand of dominant parasitic wasps such as the golden wasps of the caterpillars of the grasslands is greatly increased. However, at present, only the grassland trichogramma is known as a natural host, and the grassland trichogramma only occurs in Qinghai-Tibet plateau and occurs for one year, which greatly limits the scale of population expansion of the grassland trichogramma. It is important and urgent to find a common alternative host for the meadow trichogramma and a technical system capable of expanding the propagation for multiple generations in one year.
The invention of CN109874750A (artificial breeding method of golden bee) informs that: the method comprises the following steps:
(1) Cleaning and sterilizing the finger-shaped tube, and airing;
(2) Selecting larvae or pupae of bees or wasps as alternative hosts, and placing the alternative hosts into the middle lower part or the bottom part of the finger-shaped tube;
(3) Inserting fertilized golden-small bees into the finger-shaped pipe, wherein the number ratio of the golden-small bees to the substituted hosts is 1:1-1:5, using a cotton wool plug to cover the open end of the finger-shaped pipe after the bee insertion is finished, and placing the finger-shaped pipe into an artificial climate box for culture;
the culture conditions in the artificial climate box are as follows: the relative humidity is 30% -70%, the temperature is 15-32 ℃, and the photoperiod L8-16:D16-8.
Disclosure of Invention
The invention aims to solve the technical problem of providing a method for breeding parasitic wasps by using cabbage caterpillar pupas to replace grassland caterpillar pupas, which is used for biological control of grassland caterpillar on grasslands by breeding grassland caterpillar wasps by using the cabbage caterpillar pupas which are more widely distributed and easier to obtain.
In order to solve the technical problems, the invention provides an indoor propagation method for a meadow caterpillar wasp, which breeds the wasp by using cabbage caterpillar pupae, and comprises the following steps:
1) Treating cabbage caterpillar pupae:
feeding cabbage caterpillar larvae until pupation, sterilizing and heating the larvae after 48+/-4 hours of pupation, wherein the treated cabbage caterpillar larvae are used as hosts;
the purpose of this step 1) is to enable the cabbage caterpillar pupae to be successfully parasitized by the grassland caterpillar golden bees;
2) Inoculating parasitic wasp parasitism:
inserting parasitic wasps into the treated cabbage caterpillar pupae as the host obtained in the step 1) to carry out parasitic action at room temperature under natural light for 4-12 hours; the parasitic wasp is a grassland caterpillar golden wasp after mating (after full mating);
the inoculation mode is any one of the following:
mode a, inoculum size was set as: 1 parasitic wasp is connected to each 1 treated cabbage caterpillar pupa, and the parasitic cabbage caterpillar pupa is taken out after the set parasitic time is reached;
mode B, inoculum size was set as: the number of the parasitic wasps is half of that of the treated cabbage caterpillar pupas, and then the parasitic cabbage caterpillar pupas are taken out after the set parasitic time is reached;
the parasitic cabbage caterpillar pupae are named as parasitic rear pupae;
description: after the bee is parasitic, a obvious black point exists on the host pupae after the parasitic (pricking), so as to judge whether the cabbage caterpillar pupae are parasitic;
3) Breaking diapause of larvae:
firstly, placing the parasitic pupa obtained in the step 2) at room temperature for 6-8 days, and then, carrying out photoperiod L: d=16: 8, 8; at this time, the parasitic wasps in the pupa develop to mature larvae and start diapause; then, any one of the following methods is selected, so that the diapause of the larvae is broken:
the first method is that parasitic pupa is placed for 6 to 8 days at room temperature and then treated for 7.5 to 8.5 months at the temperature of 3 to 5 ℃,
firstly, placing parasitic pupa for 6-8 days at room temperature, treating the parasitic pupa for 13-15 days at-18 to-22 ℃, and then performing ecdysone treatment;
the photoperiod of the first and second methods is L: d=16: 8, 8;
description: the first method can basically break all the diapause of the larvae, and the second method can break part of the diapause of the larvae;
4) Eclosion of parasitic wasps:
placing the pupae obtained in the step 3) at room temperature until the pupae emerges, and carrying out photoperiod L: d=16: 8, 8; obtaining the emerged golden bees.
As the improvement of the indoor propagation method of the grassland caterpillar wasp, the invention: the disinfection and heating treatment of the step 1) is as follows: sterilizing pupa (hardened pupa shell) with 84 disinfectant diluent, and treating with hot water at 45+ -2deg.C for 10+ -1 min;
the 84 disinfectant diluent is obtained by mixing 84 disinfectant and water according to a volume ratio of 1:9.
As the further improvement of the indoor propagation method of the grassland caterpillar wasp, disclosed by the invention, the method comprises the following steps: feeding the emerged golden bees with 18-22% honey water, thereby prolonging the service life of the golden bees.
As the further improvement of the indoor propagation method of the grassland caterpillar wasp, disclosed by the invention, the method comprises the following steps: placing the emerged golden bees in a low-temperature environment of 3-5 ℃ for preservation, taking the golden bees out of the low-temperature environment every 10-15 days, feeding the golden bees with 18-22% honey water by volume concentration for 1-2 hours at room temperature, and then placing the golden bees back to the low-temperature environment of 3-5 ℃ for preservation; thereby further prolonging the life of the gold wasps.
As the further improvement of the indoor propagation method of the grassland caterpillar wasp, disclosed by the invention, the method comprises the following steps: the ecdysone treatment of the second method is as follows:
and (3) peeling off the parasitic pupa after 13-15 days of treatment at the temperature of minus 18-minus 22 ℃, taking out the parasitic bee larva, placing the parasitic bee larva on a glass slide, dripping 50g/L ecdysone solution dissolved in ethanol on the surfaces of all larva groups, and rotating the larva groups by using a soft brush to ensure that all the larva are fully contacted with the ecdysone solution.
As the further improvement of the indoor propagation method of the grassland caterpillar wasp, disclosed by the invention, the method comprises the following steps: the parasitism of the step 2), the cultivation of the step 3) and the placement of the step 4) are as follows: the relative humidity is 70+/-5% and the temperature is 26+/-1 ℃.
The relative humidity of the preservation of the low-temperature environment of the gold wasps after the treatment of the first method, the treatment of the second method and the eclosion in the step 3) is 70+/-5 percent.
In step 1) of the present invention: cabbage caterpillar larvae collected from the field are placed in an indoor nylon net bag for feeding, and mature larvae around the opening of the net bag are collected.
In the step 2) of the invention, the parasitic time is controlled to be 4-12 hours, and the parasitic rate is possibly lower below 4 hours; higher than 12 hours also results in reduced parasitic success (possibly due to parasitic bees multiple times of parasitism).
In summary, the technical scheme adopted by the invention is as follows: the treated cabbage caterpillar pupae is used as a substitute host to breed parasitic wasps in the grassland caterpillar pupae stage, which comprises (1) obtaining and treating the cabbage caterpillar pupae; (2) breeding the grassland trichogramma.
The invention has the technical effects that:
1. the cabbage caterpillar pupae can be successfully parasitic by sterilizing and heating the cabbage caterpillar pupae. The cabbage caterpillars are used as vegetable pests which are widely distributed in China, have more vegetable pests and are easier to obtain, and can be used as a substitute host of the meadow caterpillars and the meadow bee population propagation scale can be improved.
2. By low-temperature treatment and ecdysone treatment, diapause of the mature larvae of partial or all grassland artemia golden bees can be broken, so that the breeding for a plurality of generations in one year can be realized, and the population expansion scale can be improved.
Detailed Description
The invention will be further described with reference to the following specific examples, but the scope of the invention is not limited thereto:
the following examples are illustrative of the invention and are not intended to limit the scope of the invention. The technical means used in the examples are conventional means known to those skilled in the art, and all raw materials used are commercially available.
Example 1, acquisition and treatment of cabbage caterpillar pupae, comprising the following steps:
1.1, cabbage caterpillar feeding: fresh cabbage leaves which are not treated by pesticides are placed in a nylon mesh bag with the size of 30 multiplied by 45cm and the size of 18 meshes, cabbage caterpillar larvae in different ages collected in the field are placed in the mesh bag, and the mouth of the bag is tied up by nylon ropes at the mouth of the mesh bag and hung indoors. The feces in the bag are removed daily and fresh cabbage leaves are replaced, wherein the number of cabbage leaves is determined according to the number of cabbage caterpillars, and the cabbage leaves are preferably eaten every day.
1.2, collecting mature larvae: by utilizing the upward climbing characteristic of the mature larva of the cabbage caterpillar, the mature larva is collected around the mesh bag port every day, the filaments wound around the abdomen of the larva are picked up by sharp objects such as forceps, the larva is taken down and placed in a plastic square box with 138 multiplied by 85 multiplied by 35mm and a plurality of layers of water absorbing tissues are placed, and the larva is placed for 2 days at room temperature, so that the cabbage caterpillar larva pupates.
Description: the steps 1.1 and 1.2 are conventional steps.
1.3, cabbage caterpillar pupa treatment:
selecting cabbage caterpillar pupa with complete appearance and hardened pupa shell (without obvious deformation when being pinched by hands), removing residues such as faeces and leaves on the surface of the pupa, placing the cabbage caterpillar pupa in a clean beaker, adding purified water, adding the liquid level to cover the host, slightly shaking the beaker for 1 minute, and pouring out liquid in the beaker; adding 84 disinfectant diluent, wherein the liquid level is suitable for covering the host, gently shaking the beaker for 2 minutes, and pouring out the liquid in the beaker; adding purified water, preferably covering the liquid level with the host, gently shaking the beaker for 1 min, and pouring out the liquid in the beaker; obtaining sterilized cabbage caterpillar pupae;
adding purified water into the other beaker, wherein the liquid level is suitable for covering the host, heating the water to 45 ℃ by using a water bath kettle, putting the sterilized cabbage caterpillar pupa, and heating the cabbage caterpillar pupa in the water bath for 10 minutes; pouring out the liquid in the beaker, putting the pupae on a plurality of layers of water-absorbing tissues, naturally airing (generally naturally airing for 10-15 minutes), and taking the treated cabbage caterpillar pupae as a host; is used for parasitizing or temporarily storing in a refrigerator at 4 ℃.
The 84 disinfectant diluent is 10% (volume ratio) 84 disinfectant, namely 84 disinfectant and water are mixed according to a volume ratio of 1:9.
Example 2 breeding a grassland trichogramma, comprising the steps of:
2.1, optionally in any of the following ways:
in the mode A, 1 treated cabbage caterpillar pupae (obtained in the step 1.3 of the example 1) are put into a plastic drosophila tube with the diameter of 25 multiplied by 95mm, 1 head of fully mated grassland caterpillar golden bee females are connected, and a cotton plug is plugged. The capillary glass tube is vertically inserted into 20% (volume ratio) of honey water, the lower part of the capillary tube is filled with liquid and then inserted into and passes through the cotton plug to be positioned in the plastic drosophila tube, and the cotton plug is exposed out of the lower end of the capillary tube positioned in the plastic drosophila tube by about 5mm, so that nutrition is provided for parasitic bees. Until parasitization is complete (typically about 4-12 hours); taking out the parasitic cabbage caterpillar pupae.
Mode B, putting a plurality of treated cabbage caterpillar pupas (obtained in step 1.3 of example 1) into a plastic box according to parasitic wasps: inoculating fully mated grassland artemia golden bee female bees in the ratio of the number of cabbage caterpillar pupae to 1:2, placing cotton dipped with 20% of honey water, and providing nutrition for parasitic bees. Parasitic wasp parasitizing time is 4-12 hours (preferably 8 hours), and when the set parasitizing time is reached, the parasitic cabbage caterpillar pupae are taken out.
The cabbage caterpillar pupae that has been parasitized is named parasitized rear pupae.
The environments parasitic in the above embodiments a and B are set as follows: the relative humidity is 70+/-5%, the temperature is 26+/-1 ℃, and natural illumination is carried out.
After the bee is parasitic, a black spot is obvious after the parasitic (pricking) on the host pupae, so that whether the cabbage caterpillar pupae is parasitic or not is judged.
In the mode B, cotton dipped with 20% honey water is repeatedly used after being placed in the plastic box.
2.2, placing the parasitic pupa at room temperature for 7 days, wherein the cultivation environment is as follows: relative humidity 70±5%, temperature 26±1 ℃, photoperiod L: d=16: 8, 8; at this time, the parasitic wasps in the pupa develop to mature larvae and start diapause, and then any one of the following methods is selected, so that the diapause of the larvae is broken:
placing the parasitic pupa placed at room temperature for 7 days in a refrigerator at 4 ℃ for preservation, taking out after 8 months, and placing at room temperature until the parasitic pupa becomes pupa;
placing the parasitic pupa placed at room temperature for 7 days in a refrigerator at the temperature of minus 20 ℃ for 2 weeks, peeling off the host pupa, taking out the parasitic bee larva, placing the parasitic bee larva on a glass slide, and dripping ecdysone ethanol solution with the concentration of 50g/L on the surfaces of all larva groups; the larva group is rotated by using a soft brush, so that all the larva are fully contacted with the ecdysone solution, and the larva is placed in a 25X 95mm plastic drosophila tube after treatment, and is placed at room temperature until pupation occurs.
The Ecdysone (Ecdysone) is C 27 H 44 O 6 . Generally, 100. Mu.l of ecdysone ethanol solution is used for every 30 to 100 parasitic post-pupae.
The conditions of the refrigerator storage at 4 ℃ and the pupation at room temperature in the first method are as follows: relative humidity 70±5%, photoperiod L: d=16: 8, 8;
the conditions of the refrigerator low-temperature treatment at the temperature of minus 20 ℃ and the pupation standing at the room temperature in the second method are as follows: relative humidity 70±5%, photoperiod L: d=16: 8.
2.3, collecting the meadow trichogramma pupae obtained in the step 2.2, placing the meadow trichogramma pupae in a 25 multiplied by 95mm plastic drosophila tube or plastic round box, placing the meadow trichogramma pupae at room temperature for a plurality of days (generally about 6-9 days), feeding 20% honey water after eclosion, and starting a new cycle of parasitization.
When the hosts are insufficient, the parasitic wasps can be stored in a refrigerator at the temperature of 4 ℃ and fed with 20% honey water, so that the lives of the parasitic wasps are prolonged, and the parasitic wasps are specifically: the emerged golden bees are put into a refrigerator at the temperature of 4 ℃ for preservation, every 10-15 days, the golden bees are taken out of the refrigerator and fed with 20% honey water at room temperature for 1-2 hours, and then are put back into the refrigerator at the temperature of 4 ℃ for preservation. Adult bees can survive for 2 months in a refrigerator at 4 ℃.
The conditions of the eclosion and the refrigerator at 4 ℃ are as follows: relative humidity 70±5%, photoperiod L: d=16: 8.
description: after eclosion, if the bees are not stored in a refrigerator at 4 ℃, only 20% of honey water is fed, and the survival period of the adult bees is only about 3-10 days.
Comparative example 1: 84 disinfectant sterilization and hot water treatment in step 1.3 of example 1 were omitted, and the rest was the same as example 1. Example 2 was then performed.
That is, step 1.3 of example 1 is changed to:
1.3, cabbage caterpillar pupa treatment: selecting cabbage caterpillar pupa with complete appearance and hardened pupa shell, removing residue such as faeces and leaves on the surface of the pupa, placing in a clean beaker, adding purified water, and pouring out liquid in the beaker after gently shaking the beaker for 1 minute when the liquid level is suitable for covering the host; placing the pupae on a plurality of layers of water-absorbing tissues, naturally airing, and obtaining the product named as 'untreated cabbage caterpillar pupae'.
Indoor propagation was performed as in example 2, with untreated cabbage caterpillar pupae as host.
Under laboratory conditions, the parasitic cabbage caterpillar pupae of the grassland caterpillar gold-hornet, after being parasitic, the parasitic position of the untreated cabbage caterpillar pupae starts to turn black and gradually spread to the whole pupae, finally the whole pupae turns black, the inside of the pupae starts to rot, parasitic larva of the bee can be seen occasionally after being stripped, but the larva cannot continue to develop and die in the pupae, and the parasitic success rate is 0%. Either mode a or mode B is the result described above.
The corresponding results of example 2 of the present invention are: after the treated cabbage caterpillar pupae are parasitized, the blackening degree of the pupae is obviously reduced and the pupae cannot be rotted, the parasitic wasp larva in the pupae can continue to develop, the average parasitizing success rate of the method B is 45.5+/-6.32% (average value+/-standard deviation, the same applies below), and the parasitizing success rate is obviously higher than that of the untreated cabbage caterpillar pupae (P is less than 0.01).
Success rate of parasitism = number of pupae successfully parasitized (parasitic bees can develop into mature larvae)/number of pupae used for parasitism.
Comparative example 2, cancellation of "then any one of the following methods was selected in step 2.2 of example 2, thereby achieving breaking of diapause of larvae", i.e. parasitic wasps in pupae that developed to mature larvae and began diapause were directly subjected to step 2.3;
the results obtained were: the average pupation rate was 0 after continuing to stand for 2 weeks under indoor conditions.
The average emergence rate after the treatment by the method is obviously higher than that of the comparative example 2; wherein, the average emergence rate of the first method of example 2 after 8 months of treatment at 4 ℃ in a refrigerator is 82.4+/-4.57% (P < 0.01), and the average pupation rate of the second method of example 2 after 2 weeks of treatment at-20 ℃ in a refrigerator and ecdysone treatment is 9.58+/-2.27% (P=0.018).
In the invention process, the following comparative experiments are also carried out: the 84 sterilization solution sterilization in step 1.3 of example 1 was omitted, only the heat treatment was performed, and the rest was the same as example 1. Alternatively, the heat treatment in step 1.3 of example 1 was omitted, and only 84 sterilization solutions were used for sterilization, and the rest was the same as example 1. Example 2 was then performed. The average parasitic success rate of the method B is less than 15 percent.
Finally, it should also be noted that the above list is merely a few specific embodiments of the present invention. Obviously, the invention is not limited to the above embodiments, but many variations are possible. All modifications directly derived or suggested to one skilled in the art from the present disclosure should be considered as being within the scope of the present invention.
Claims (7)
1. An indoor propagation method for a grassland caterpillar wasp is characterized by comprising the following steps: and breeding the wasp by using cabbage caterpillar pupae.
2. The indoor propagation method of a grassland caterpillar wasp according to claim 1, comprising the steps of:
1) Treating cabbage caterpillar pupae:
feeding cabbage caterpillar larvae until pupation, sterilizing and heating the larvae after 48+/-4 hours of pupation, wherein the treated cabbage caterpillar larvae are used as hosts;
2) Inoculating parasitic wasp parasitism:
inserting parasitic wasps into the treated cabbage caterpillar pupae as the host obtained in the step 1) to carry out parasitic action at room temperature under natural light for 4-12 hours; the parasitic wasp is a mated grassland caterpillar wasp;
the inoculation mode is any one of the following:
mode a, inoculum size was set as: 1 parasitic wasp is connected to each 1 treated cabbage caterpillar pupa, and the parasitic cabbage caterpillar pupa is taken out after the set parasitic time is reached;
mode B, inoculum size was set as: the number of the parasitic wasps is half of that of the treated cabbage caterpillar pupas, and then the parasitic cabbage caterpillar pupas are taken out after the set parasitic time is reached;
the parasitic cabbage caterpillar pupae are named as parasitic rear pupae;
3) Breaking diapause of larvae:
firstly, placing the parasitic pupa obtained in the step 2) at room temperature for 6-8 days, and then, carrying out photoperiod L: d=16: 8, 8; at this time, the parasitic wasps in the pupa develop to mature larvae and start diapause; then, any one of the following methods is selected, so that the diapause of the larvae is broken:
the first method is that parasitic pupa is placed for 6 to 8 days at room temperature and then treated for 7.5 to 8.5 months at the temperature of 3 to 5 ℃,
firstly, placing parasitic pupa for 6-8 days at room temperature, treating the parasitic pupa for 13-15 days at-18 to-22 ℃, and then performing ecdysone treatment;
the photoperiod of the first and second methods is L: d=16: 8, 8;
4) Eclosion of parasitic wasps:
placing the pupae obtained in the step 3) at room temperature until the pupae emerges, and carrying out photoperiod L: d=16: 8, 8; obtaining the emerged golden bees.
3. The indoor propagation method of the grassland caterpillar wasp according to claim 2, wherein the method comprises the following steps: the disinfection and heating treatment of the step 1) is as follows: firstly, sterilizing the pupa by using 84 disinfectant diluent, and then treating the pupa for 10+/-1 min by using hot water at 45+/-2 ℃;
the 84 disinfectant diluent is obtained by mixing 84 disinfectant and water according to a volume ratio of 1:9.
4. A method for indoor propagation of a grassland trichogramma according to claim 2 or 3, characterized in that: feeding the emerged golden bees with 18-22% honey water, thereby prolonging the service life of the golden bees.
5. The indoor propagation method of the grassland trichogramma according to any one of claims 2 to 4, which is characterized in that: placing the emerged golden bees in a low-temperature environment of 3-5 ℃ for preservation, taking the golden bees out of the low-temperature environment every 10-15 days, feeding the golden bees with 18-22% honey water by volume concentration for 1-2 hours at room temperature, and then placing the golden bees back to the low-temperature environment of 3-5 ℃ for preservation; thereby further prolonging the life of the gold wasps.
6. The indoor propagation method of the grassland trichogramma according to any one of claims 2 to 5, characterized in that the ecdysone treatment of the method two is:
and (3) peeling off the parasitic pupa after 2-5 days of treatment at-18 to-22 ℃, taking out the parasitic bee larva, placing the parasitic bee larva on a glass slide, dripping 50g/L ecdysone solution dissolved in ethanol on the surfaces of all larva groups, and rotating the larva groups by using a soft brush to ensure that all the larva are fully contacted with the ecdysone solution.
7. The indoor propagation method for the meadow caterpillar wasps according to any one of claims 2 to 5, which is characterized in that:
the parasitism of the step 2), the cultivation of the step 3) and the placement of the step 4) are as follows: relative humidity 70+ -5%, temperature 26+ -1deg.C;
the relative humidity of the preservation of the low-temperature environment of the gold wasps after the treatment of the first method, the treatment of the second method and the eclosion in the step 3) is 70+/-5 percent.
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