CN102599109A - Simple method for purifying rotifers in batch - Google Patents
Simple method for purifying rotifers in batch Download PDFInfo
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- CN102599109A CN102599109A CN201210047744XA CN201210047744A CN102599109A CN 102599109 A CN102599109 A CN 102599109A CN 201210047744X A CN201210047744X A CN 201210047744XA CN 201210047744 A CN201210047744 A CN 201210047744A CN 102599109 A CN102599109 A CN 102599109A
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Abstract
A simple method for purifying rotifers in batch belongs to the technical field of biological cultivation and includes the steps of catching, initial filtering, cleaning, treatment with medicine, standing, separating and liquid medicine removal. The simple method has the advantages that purification of rotifers in stock solution needs no precision lab equipment and is simple, and the method is applicable to large-scale production of the rotifers, is efficient, economical, practical and the like, and is of important significance to cultivation of the rotifers and juveniles.
Description
Technical field
The invention belongs to the biological technical field of cultivating, relate in particular to the simple and easy method of a kind of mass purification wheel animalcule.
Background technology
In the cultivating process of fry; The live body wheel animalcule is the indispensable animal bait of postlarva; Enlarge in the cultivating process, need the bigger pure wheel animalcule of relative quantity, need a large amount of pure wheel animalcules to throw something and feed as the provenance (underlying group) of wheel animalcule expansion cultivation or in fry rearing wheel animalcule.
At present, carry out the provenance source that wheel animalcule enlarge to cultivate three kinds of approach are arranged.
The one, carry out the wheel animalcule monoclonal: under the laboratory condition, single wheel animalcule is selected, put into one by one in the small container of 50mL triangular flask and cultivate at microscopically.Needed 10 days to 30 days or cultivation (one-level cultivation) for more time.The wheel animalcule that monoclonal cultivates also need pass through intermediate rearing (secondary cultivation) and arrive yielding ability expansion cultivation (three grades of cultivations) again.In these three breeding phases, the provenance in each stage (underlying group) lazy weight will obtain the big cultivation colony of quantity and need pass through the long time.
The single-stage wheel animalcule cultivation time is long, must receive protozoa or scratch the pollution of foot type, finally leads to the failure and can not obtain a large amount of pure wheel animalcules.To the wheel animalcule requirement that satisfies the fry large-scale production, wheel animalcule monoclonal to wheel animalcule large-scale production just approximately needs 2 months to 3 months time from monoclonal.This mode success rate is low, time-consuming, effort, efficient are low.
The 2nd, buy the wheel worm's ovum and hatch and obtain.1. this approach exists buys difficulty, and most breed dealers do not know manufacturer; 2. cost an arm and a leg, cost is high; 3. take turns worm's ovum and exist incubation rate low sometimes, cost performance is low; 4. purchase wheel animalcule incompatibility local environment, cause introducing a fine variety failure, have a big risk.
The 3rd, directly make a big purchase in large quantities or fish for: no matter be the wheel animalcule of making a big purchase in large quantities or fishing for, be the free wheel worm after outdoor production is collected at present, mixing usually has nematode, scratches foot type adult and scratches the foot type six limb young, the shrimp young, assorted shrimp, protozoa etc.
The bulk purchase wheel animalcule has part or most of dead after long-distance transport, can not directly throw something and feed to postlarva as the erbium material, more unsuitablely directly enlarges the provenance of cultivating as yielding ability.1. these wheel animalcules contain various protozoas and scratch sufficient type, and 2. the packed and transported time is unsuitable long; Every bag of Packing Unit is little, and the long-distance transportation cost is high, and 3. survival rate is not high, and the above transportation survival rate of temperature 25 degree is lower; 4. environmental suitability is poor.
Want to obtain comparatively difficulty of the pure wheel animalcule of live body in enormous quantities aborning.
The one, the physical filtering separation difficulty: the individuality big with the wheel animalcule individual difference can be able to filter through screen cloth.The same with wheel animalcule size perhaps than protozoas such as the little individuality of wheel animalcule such as some duricrust worms, just is difficult to be added on filter and has distinguished.
The 2nd, drug effect and separation difficulty: wheel animalcule belongs to shell-fish with scratching the foot class, requires medicine to have the selection property to similar xenogenesis.A lot of medicines comprise wheel animalcule interior all kill or can only partly kill other mix polypide, or wheel animalcule is killed and other polypides are not killed, also just can not reach the purpose of purifying wheel animalcule.
At present in secondary, three grades of wheel animalcules are cultivated; Owing to have no idea to obtain the pure wheel animalcule underlying group of q.s; Cause the scale fry to produce required wheel animalcule and can't realize that cause survival rate to reduce significantly thereby cause hatching not have a large amount of wheel animalcules to ingest, the scale fry produces and can not carry out.In the production of seawater fry, show especially outstandingly.
Summary of the invention
According to above deficiency; The present invention provides the simple and easy method of a kind of mass purification wheel animalcule; Present technique is to carry out purifying to the various small-sized hydrobiological impure wheel animalcules that contain of in the pond, fishing for; The wheel animalcule of mass purification can be satisfied and is used for productive wheel animalcule and enlarges and cultivate, and promptly before protozoa occurs, cultivates more a large amount of wheel animalcules in 7～10 days.
To achieve these goals, the technical scheme that adopts of the present invention is: the simple and easy method of a kind of mass purification wheel animalcule may further comprise the steps: fish for, filter just, cleaning, drug treating, leave standstill, separate, dispel soup; The detail operations of above-mentioned steps is following:
The first step, fish for: in the pond with 200 mesh sieve thin,tough silk diddle-net artificial fish for or less than the water pumper of 0.5KW water be drawn into 200 mesh sieve tulle bag filtrations through small-power obtain wheel animalcule; The wheel animalcule that is obtained mixes usually has nematode, scratch foot type adult and scratch the foot type six limb young, the shrimp young, assorted shrimp, protozoa etc.;
Second step, filter just: with 40 orders or 60 orders diddle-net, remove weeds, big individual aquatile, obtain mixing the wheel animalcule of various little individual animals stoste is arranged like large-scale foreign material such as the shrimps of mixing;
The 3rd step, cleaning: use 200 mesh sieve thin,tough silk diddle-net or 200 mesh sieve tulle bag filtration stostes, rinse well, put into then in clean 20 liters～50 liters separatory funnel of sterilization with the clean water after the sterilization;
The 4th step, drug treating: add the processing medicine of 0.01ppm to 0.015ppm in the transparent separatory funnel,, stop the rotation of water in the funnel then, let water in the funnel is static at short notice to get off with clean glass rod stirring 10 seconds～30 seconds;
Said processing medicine is a fluorine cyanogen chrysanthemum fat, and its consumption calculates according to water body in the separatory funnel and medication concentration.
The 5th goes on foot, leaves standstill: leaving standstill 5 minutes～10 minutes through after the drug treating, is not that the various animals of wheel animalcule are dead one after another, and sinking to funnel bottom becomes hypostasis; And wheel animalcule can be not dead, swims in the separatory funnel surface treatment liquid;
The 6th step, separate: open the switch of separatory funnel, collect hypostasis with Plastic Drum, treatment fluid no longer includes till the foreign material to the separatory funnel, has closed switch; If above-mentioned stoste is too dense, hypostasis possibly have more wheel animalcule, can put into once more in the funnel, adds clean water and carries out static separation once more;
The 7th the step, dispel soup: the said soup process of dispeling is: open the separatory funnel switch; Treatment fluid in the separatory funnel is flow in the 200 purpose bolting silk mesh bags; With clean Plastic Drum then, treat that the treatment fluid in the separatory funnel all flows to the bolting silk mesh bag below, clean about 3 times with clean water behind the detoxicating; Obtain the wheel animalcule of purifying, promptly can be used for productive expansion and cultivate.Said treatment fluid is water and wheel animalcule mixing material.
The invention has the beneficial effects as follows: do not need the laboratory precision equipment; With wheel animalcule purifying in the stoste, operating process is simple, is suitable for the large-scale production wheel animalcule; Having characteristics such as efficient, economic, practical, all is to have crucial meaning to wheel animalcule cultivation and postlarva cultivation.
Embodiment
Further the present invention is explained below in conjunction with embodiment:
The simple and easy method of embodiment, a kind of mass purification wheel animalcule, this method has the following steps: fish for, filter just, cleaning, drug treating, leave standstill, separate, dispel soup.The detail operations of said step is following: the first step, fish for: in the pond with 200 mesh sieve thin,tough silk diddle-net artificial fish for or less than the water pumper of 0.5KW water be drawn into 200 mesh sieve tulle bag filtrations through small-power obtain wheel animalcule; The wheel animalcule that is obtained mixes usually has nematode, scratch foot type adult and scratch the foot type six limb young, the shrimp young, assorted shrimp, protozoa etc.Second step, filter just: with 40 orders or 60 orders diddle-net, remove weeds, big individual aquatile, obtain mixing the wheel animalcule of various little individual animals stoste is arranged like large-scale foreign material such as the shrimps of mixing.The 3rd step, cleaning: use 200 mesh sieve thin,tough silk diddle-net or 200 mesh sieve tulle bag filtration stostes, rinse well, put into then in clean 20 liters～50 liters separatory funnel of sterilization with the clean water after the sterilization.The 4th step, drug treating: add the processing medicine of 0.01ppm to 0.015ppm in the separatory funnel,, stop the rotation of water in the funnel then, let water in the funnel is static at short notice to get off with clean glass rod stirring 10 seconds～30 seconds.Said processing medicine fluorine cyanogen chrysanthemum fat consumption calculates according to water body in the triangle separatory funnel and medication concentration.The 5th step, leave standstill: leaving standstill through 5～10 minutes, is not that the various animals of wheel animalcule are dead one after another, and sinking to funnel bottom becomes hypostasis; And wheel animalcule can be not dead, swims in the separatory funnel surface treatment liquid.The 6th step, separate: open the switch of separatory funnel, collect hypostasis with Plastic Drum, treatment fluid no longer includes till the foreign material to the separatory funnel, has closed switch.Further, if above-mentioned stoste is too dense, hypostasis possibly have more wheel animalcule, can put into once more in the funnel, adds clean water and carries out static separation once more.The 7th the step, dispel soup: open switch; Treatment fluid in the separatory funnel is flow in the 200 purpose bolting silk mesh bags; With clean Plastic Drum then, treat that the treatment fluid in the separatory funnel all flows to the bolting silk mesh bag below, clean about 3 times with clean water behind the detoxicating; Obtain the wheel animalcule of purifying, promptly can be used for productive expansion and cultivate.Said treatment fluid is water and wheel animalcule mixing material.
The present invention is not limited to above-mentioned embodiment, and all are based on technical conceive of the present invention, and the structural improvement of having done all falls among protection scope of the present invention.
Claims (3)
1. the simple and easy method of a mass purification wheel animalcule, characteristic is may further comprise the steps: fish for, filter just, cleaning, drug treating, leave standstill, separate, dispel soup; Detail operations is following: the first step, fish for: in the pond with 200 mesh sieve thin,tough silk diddle-net artificial fish for or less than the water pumper of 0.5KW water be drawn into 200 mesh sieve tulle bag filtrations through small-power obtain wheel animalcule; The wheel animalcule that is obtained mixes usually has nematode, scratch foot type adult and scratch the foot type six limb young, the shrimp young, assorted shrimp, protozoa etc.; Second step, filter just: with 40 orders or 60 orders diddle-net, remove weeds, big individual aquatile, obtain mixing the wheel animalcule of various little individual animals stoste is arranged like large-scale foreign material such as the shrimps of mixing; The 3rd step, cleaning: use 200 mesh sieve thin,tough silk diddle-net or 200 mesh sieve tulle bag filtration stostes, rinse well, put into then in clean 20 liters～50 liters separatory funnel of sterilization with the clean water after the sterilization; The 4th step, drug treating: add the processing medicine of 0.01ppm to 0.015ppm in the transparent separatory funnel,, stop the rotation of water in the funnel then, let water in the funnel is static at short notice to get off with clean glass rod stirring 10 seconds～30 seconds; The 5th goes on foot, leaves standstill: leaving standstill 5 minutes～10 minutes through after the drug treating, is not that the various animals of wheel animalcule are dead one after another, and sinking to funnel bottom becomes hypostasis; And wheel animalcule can be not dead, swims in the separatory funnel surface treatment liquid; The 6th step, separate: open the switch of separatory funnel, collect hypostasis with Plastic Drum, treatment fluid no longer includes till the foreign material to the separatory funnel, has closed switch; If above-mentioned stoste is too dense, hypostasis possibly have more wheel animalcule, can put into once more in the funnel, adds clean water and carries out static separation once more; The 7th the step, dispel soup: the said soup process of dispeling is: open the separatory funnel switch; Treatment fluid in the separatory funnel is flow in the 200 purpose bolting silk mesh bags; Below then with clean Plastic Drum; Treat that the treatment fluid in the separatory funnel all flows to the bolting silk mesh bag, clean about 3 times, obtain the wheel animalcule of purifying with clean water behind the detoxicating.
2. the simple and easy method of a kind of mass purification wheel animalcule according to claim 1 is characterized in that said processing medicine is a fluorine cyanogen chrysanthemum fat, and its consumption calculates according to water body in the separatory funnel and medication concentration.
3. the simple and easy method of a kind of mass purification wheel animalcule according to claim 1 is characterized in that the said drug treating time: 5～10 minutes.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103141441A (en) * | 2012-10-23 | 2013-06-12 | 中国水产科学研究院东海水产研究所 | Method for cultivating rotifers by using dried yeast and Antarctic krill meal |
| CN104304192A (en) * | 2014-10-26 | 2015-01-28 | 中盐工程技术研究院有限公司 | Simple method for increasing hatching rate of resting eggs of sea rotifers |
| CN104396896A (en) * | 2014-12-09 | 2015-03-11 | 南京师范大学 | Method for rapidly producing wheel animalcule and resting eggs of wheel animalcule |
| CN107079854A (en) * | 2017-04-10 | 2017-08-22 | 中国水产科学研究院南海水产研究所 | A kind of purifying protecting method of water rotifer |
| CN109819942A (en) * | 2019-03-15 | 2019-05-31 | 山东省林业科学研究院 | A kind of pine needle sheath cecidomyiia Overwintering Larvae separation method and its application |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1989803A (en) * | 2005-12-26 | 2007-07-04 | 中国水产科学研究院东海水产研究所 | Method for killing copepods in rotaria culture |
| CN101595856A (en) * | 2009-06-25 | 2009-12-09 | 大连水产学院 | A kind of freshwater rotifer common nutrient solution and method for making thereof |
| CN101653106A (en) * | 2009-09-22 | 2010-02-24 | 中国水产科学研究院渔业机械仪器研究所 | Closed circulating rotifer continuous culturing system |
| CN101692805A (en) * | 2009-10-13 | 2010-04-14 | 中国水产科学研究院淡水渔业研究中心 | Method for collecting rotifers, Cladocera and copepods zooplankton |
| CN102106291A (en) * | 2011-01-31 | 2011-06-29 | 中国水产科学研究院东海水产研究所 | Specific-pathogen-free litopenaeus vannamei seedlings breeding method for southern China |
-
2012
- 2012-02-29 CN CN201210047744.XA patent/CN102599109B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1989803A (en) * | 2005-12-26 | 2007-07-04 | 中国水产科学研究院东海水产研究所 | Method for killing copepods in rotaria culture |
| CN101595856A (en) * | 2009-06-25 | 2009-12-09 | 大连水产学院 | A kind of freshwater rotifer common nutrient solution and method for making thereof |
| CN101653106A (en) * | 2009-09-22 | 2010-02-24 | 中国水产科学研究院渔业机械仪器研究所 | Closed circulating rotifer continuous culturing system |
| CN101692805A (en) * | 2009-10-13 | 2010-04-14 | 中国水产科学研究院淡水渔业研究中心 | Method for collecting rotifers, Cladocera and copepods zooplankton |
| CN102106291A (en) * | 2011-01-31 | 2011-06-29 | 中国水产科学研究院东海水产研究所 | Specific-pathogen-free litopenaeus vannamei seedlings breeding method for southern China |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103141441A (en) * | 2012-10-23 | 2013-06-12 | 中国水产科学研究院东海水产研究所 | Method for cultivating rotifers by using dried yeast and Antarctic krill meal |
| CN103141441B (en) * | 2012-10-23 | 2014-06-04 | 中国水产科学研究院东海水产研究所 | Method for cultivating rotifers by using dried yeast and Antarctic krill meal |
| CN104304192A (en) * | 2014-10-26 | 2015-01-28 | 中盐工程技术研究院有限公司 | Simple method for increasing hatching rate of resting eggs of sea rotifers |
| CN104396896A (en) * | 2014-12-09 | 2015-03-11 | 南京师范大学 | Method for rapidly producing wheel animalcule and resting eggs of wheel animalcule |
| CN107079854A (en) * | 2017-04-10 | 2017-08-22 | 中国水产科学研究院南海水产研究所 | A kind of purifying protecting method of water rotifer |
| CN109819942A (en) * | 2019-03-15 | 2019-05-31 | 山东省林业科学研究院 | A kind of pine needle sheath cecidomyiia Overwintering Larvae separation method and its application |
| CN109819942B (en) * | 2019-03-15 | 2021-08-03 | 山东省林业科学研究院 | Pine needle cecidomyiia overwintering larva separation method and application thereof |
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| CN102599109B (en) | 2014-07-30 |
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