CN101653106A - Closed circulating rotifer continuous culturing system - Google Patents
Closed circulating rotifer continuous culturing system Download PDFInfo
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- CN101653106A CN101653106A CN200910196065A CN200910196065A CN101653106A CN 101653106 A CN101653106 A CN 101653106A CN 200910196065 A CN200910196065 A CN 200910196065A CN 200910196065 A CN200910196065 A CN 200910196065A CN 101653106 A CN101653106 A CN 101653106A
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- wheel animalcule
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Abstract
The invention provides a closed circulating rotifer continuous culturing system which comprises at least one group of rotifer culturing barrels, at least one group of rotifer culturing reinforcement barrels, a rotifer harvesting device, a water treatment device and at least one group of microalgae culturing barrels, wherein the output ends of the rotifer barrels are connected with the rotifer culturing reinforcement barrels, the output ends of the rotifer culturing reinforcement barrels are connected with the rotifer harvesting device, the rotifer harvesting device is connected with the watertreatment device, the output end of the water treatment device is connected with the microalgae culturing barrel, and the microalgae culturing barrel is communicated with the rotifer culturing barrels. The invention adopts a special microalgae culture control technology, a water treatment technology and a chemostat theory, realizes the recycle of rotifer culture water the continuous production ofrotifers, saves water resources and reduces emission of harmful substances under the condition of ensuring that the rotifers can be efficiently and stably produced; meanwhile, the invention performs nutrition reinforcement to the cultured rotifers, improves and strengthens the nutrition quality of the rotifers, and achieves the purpose of the invention.
Description
Technical field
The present invention relates to a kind of culture systems that is used to cultivate microorganism, particularly a kind of wheel animalcule continuous culture system that is used to cultivate the closed circulation of wheel animalcule.
Background technology
Wheel animalcule is the multicellular animals of a kind of universal small (0.04-2.0 micron), some advantages such as can artificially strengthen and easily digest and assimilate because but wheel animalcule self has individual little, slow, the fast High Density Cultivation of breeding of moving about, nutrition, make wheel animalcule become a kind of very important biological feed.At present in marine fish is grown seedlings, generally all the wheel animalcule that ingests behind the prelarva opening, also there is not any artificial feed can replace wheel animalcule at present, some microcapsule feedstuffs obtain certain success in prawn seed-rearing, but in fish are grown seedlings, because the fish young can not digest these microcapsule feedstuffs, it is bait that the fish young still needs with wheel animalcule.Wheel animalcule be fish, shrimp, crab class grow seedlings culture in requisite important living bait source.
At present, both at home and abroad the yielding ability of wheel animalcule is cultivated, according to the difference of incubation or operation sequence, can be divided into following several method: batch property cultivation, semicontinuous cultivation, continuous culture and feedback are cultivated.But, wheel animalcule also comes with some shortcomings in production application at present and mainly shows as: 1. a large amount of lability of cultivating, owing to along with the harmful substance of bringing into together, can cause and population density takes place in incubation often die-off in the incubation as little algae of bait; 2. a large amount of cultivate expensive because seedling is very big to the demand of wheel animalcule, and is cultivated wheel animalcule and also need a large amount of little algaes, wheel animalcule is cultivated taken considerable human and material resources and space; 3. the waste water after a large amount of the cultivation discharges wantonly, and environment is polluted, and the nitrogen in the waste water fails to make full use of simultaneously.
Because above these problems have largely restricted the wheel animalcule application and development, therefore, need a kind of wheel animalcule continuous culture system especially, remedy the existing weak point of wheel animalcule in production application.
Summary of the invention
The object of the present invention is to provide a kind of wheel animalcule continuous culture system of closed circulation, remedy the existing weak point of wheel animalcule in production application, adopt specific little algae to cultivate control technology, water technology and chemostat principle, realize the continuous production of the recycling and wheel animalcule of wheel animalcule cultivation water, can be under guaranteeing that the wheel animalcule efficient stable is produced, both save water resource, reduced emission of harmful substances again.
Technical problem solved by the invention can realize by the following technical solutions:
A kind of wheel animalcule continuous culture system of closed circulation, it is characterized in that it is used for wheel animalcule that wheel animalcule cultivates by at least one group and cultivates bucket, at least one group and be used for wheel animalcule recovering device, that wheel animalcule fortification bucket, that wheel animalcule carries out fortification is used to gather in the crops the wheel animalcule after the cultivation and comprise the water treatment facilities of physical filtering, biofiltration or disinfection etc. and at least one group and be used for specific little algae and cultivate and supply with described wheel animalcule and cultivate little algae of bucket and cultivate bucket and constitute; The output that described wheel animalcule is cultivated bucket is connected with described wheel animalcule fortification bucket, the output of described wheel animalcule fortification bucket is connected with described wheel animalcule recovering device, described wheel animalcule recovering device is connected with described water treatment facilities, the output of described water treatment facilities is cultivated bucket with described little algae and is connected, and described little algae is cultivated bucket and cultivates bucket UNICOM with described wheel animalcule.
In one embodiment of the invention, it is one group of chemostat that is used for the wheel animalcule cultivation that described wheel animalcule is cultivated bucket, cultivates in the bucket described wheel animalcule and is provided with aerator, and cultivating for wheel animalcule provides oxygen and realization immixture.
Further, described wheel animalcule is cultivated bucket and also can be provided with an adjustment control device that makes described wheel animalcule cultivation bucket maintenance water temperature as required.
Further, can add nutrition fortifier in the described wheel animalcule fortification bucket wheel animalcule is carried out fortification.
In one embodiment of the invention, described wheel animalcule recovering device adopts bolting silk or mesh to filter, and also can adopt rotation net purpose reaping machine.
Further, the aperture of described mesh is the 80-100 micron.
In one embodiment of the invention, described water treatment facilities comprises physical filtering, biofiltration or disinfection, and described physical filtering adopts sandfiltration pot, Microfilter or air supporting machine; Biofilter is adopted in biofiltration, and disinfection adopts ultraviolet sterilizer, ozone equipment or photo-catalyst device; Described water treatment facilities adopts one of above-mentioned or its combination.
In one embodiment of the invention, described little algae is cultivated the cultivation bucket that bucket is one group of little algae of post-directed training under the illumination effect, and described little algae is cultivated in the bucket and is provided with aerator, and cultivating for little algae provides carbonic acid gas and realize immixture.
Further, cultivate bucket in described little algae and be provided with micro algae culturing liquid, described micro algae culturing liquid adopts the F/2 prescription, specific little algae (being generally the seawater diatom) is cultivated finite concentration, and the algae liquid of then little algae being cultivated 50%-70% in the bucket is transported to described wheel animalcule and cultivates the bait of cultivating as wheel animalcule in the bucket.
The wheel animalcule continuous culture system of closed circulation of the present invention, adopt specific little algae to cultivate control technology, water technology and chemostat principle, realize the continuous production of the recycling and wheel animalcule of wheel animalcule cultivation water, can be under guaranteeing that the wheel animalcule efficient stable is produced, both save water resource, reduced emission of harmful substances again, simultaneously the wheel animalcule of cultivating had been carried out fortification, improve and strengthened the nutritional quality of wheel animalcule, realize purpose of the present invention.
Description of drawings
Fig. 1 is the structural representation of the wheel animalcule continuous culture system of closed circulation of the present invention.
Embodiment
For technological means, creation characteristic that the present invention is realized, reach purpose and effect is easy to understand, below in conjunction with concrete diagram, further set forth the present invention.
As shown in Figure 1, the wheel animalcule continuous culture system of circulation of the present invention sealing, it by be used for the wheel animalcule that wheel animalcule cultivates cultivate bucket 100, be used for wheel animalcule fortification bucket 200 that wheel animalcule carries out fortification, be used to gather in the crops the wheel animalcule after the cultivation wheel animalcule recovering device 300, comprise the water treatment facilities 400 of physical filtering, biofiltration or disinfection etc. and be used for specific little algae and cultivate and supply with wheel animalcule and cultivate little algae of bucket 100 and cultivate bucket 500 and constitute; Water treatment facilities 400 is made of sandfiltration pot 401 and ultraviolet sterilizer 402.
The output that wheel animalcule is cultivated bucket 100 is connected with wheel animalcule fortification bucket 200, and wheel animalcule is cultivated in the bucket 100 wheel animalcule and carries out the wheel animalcule cultivation by adding bait, and cultured wheel animalcule flows to wheel animalcule fortification bucket 200 by action of gravity with water; The output of wheel animalcule fortification bucket 200 is connected with wheel animalcule recovering device 300, add nutrition fortifier in the wheel animalcule fortification bucket 200 wheel animalcule is carried out fortification 12-24h, then wheel animalcule and water are entered into wheel animalcule recovering device 300 together, realize wheel animalcule and moisture from; Wheel animalcule recovering device 300 is connected with water treatment facilities 400, and the waste water of wheel animalcule recovering device 300 dischargings enters water treatment facilities 400 by water pump 110; The output of water treatment facilities 400 is cultivated bucket 500 with little algae and is connected, and the water after handling through water treatment facilities 400 enters into little algae and cultivates bucket 500, and little algae is cultivated bucket 500 little algae of post-directed training under the illumination effect; Little algae cultivation bucket 500 is cultivated barrel 100 UNICOMs with wheel animalcule, algae liquid in little algae cultivation bucket 500 is transported to wheel animalcule by water pump 120 cultivates in the bucket 100, as the bait of wheel animalcule cultivation.
It is one group of chemostat that is used for the wheel animalcule cultivation that wheel animalcule is cultivated bucket 100, cultivates in the bucket 100 wheel animalcule and is provided with aerator, and in the present embodiment, described aerator is a gas stone 101, and cultivating for wheel animalcule provides oxygen and realization immixture; Wheel animalcule is cultivated bucket 100 also can be provided with an adjustment control device that makes described wheel animalcule cultivation bucket maintenance water temperature as required.
Add nutrition fortifier in the wheel animalcule fortification bucket 200, described nutrition fortifier is the n-3 highly unsaturated fatty acid; The time of carrying out fortification of wheel animalcule in wheel animalcule fortification bucket 200 is 12h-24h.
Wheel animalcule recovering device 300 adopts bolting silks or mesh to filter, and also can adopt rotation net purpose reaping machine, and the aperture of described mesh is the 80-100 micron, realize wheel animalcule and moisture from.
Water treatment facilities 400 comprises physical filtering, biofiltration or disinfection, and described physical filtering adopts sandfiltration pot, Microfilter or air supporting machine; Biofilter is adopted in biofiltration, and disinfection adopts ultraviolet sterilizer, ozone equipment or photo-catalyst device; Water treatment facilities 400 also can adopt one of above-mentioned or its combination; In the present embodiment, water treatment facilities 400 is made of sandfiltration pot 401 and ultraviolet sterilizer 402, is introduced into sandfiltration pot 401 and carries out physical filtering, passes through ultraviolet sterilizer 402 sterilizations again.
Little algae is cultivated the cultivation bucket that bucket 500 is one group of little algae of post-directed training under the illumination effect, and little algae is cultivated in the bucket 500 and is provided with aerator, and in the present embodiment, described aerator is a gas stone 501, and cultivating for little algae provides carbonic acid gas and realize immixture.
Cultivate bucket 500 in little algae and be provided with micro algae culturing liquid, described micro algae culturing liquid adopts the F/2 prescription, specific little algae (being generally the seawater diatom) is cultivated finite concentration, and the algae liquid of then little algae being cultivated 50%-70% in the bucket is transported to described wheel animalcule and cultivates the bait of cultivating as wheel animalcule in the bucket.
In the present embodiment, the gentle stone of gas stone 101 501 is connected to each other by pipeline, adopts air pump respectively air to be imported wheel animalcule and cultivates bucket 100 and little algae and cultivate bucket 500 and carry out aeration.
During work, wheel animalcule is cultivated in the bucket 100 wheel animalcule and carries out the wheel animalcule cultivation by adding bait, wheel animalcule is cultivated has gas stone 101 to mix for the wheel animalcule cultivation provides oxygen and realization by aeration in the bucket 100, cultured wheel animalcule (each 40%-60%) flows to wheel animalcule fortification bucket 200 by action of gravity with water, in wheel animalcule fortification bucket 200, add nutrition fortifier (as the n-3 highly unsaturated fatty acid) wheel animalcule is carried out fortification 12-24h, then wheel animalcule and water are entered into together wheel animalcule recovering device 300 realize wheel animalcules and moisture from, the waste water of discharging enters sandfiltration pot 401 by water pump and carries out physical filtering, pass through ultraviolet sterilizer 402 sterilizations again, water after treatment enters into little algae and cultivates bucket 500, little algae is cultivated has gas stone 501 to mix by aeration in the bucket 500, train little algae simultaneously under the illumination effect for a certain area, little concentration of algae reaches (50-200) * 10
4Behind the ind/mL, the algae liquid of little algae being cultivated 50%-70% in the bucket 500 is transported to wheel animalcule by water pump and cultivates the bait of cultivating as wheel animalcule in the bucket 100.
Cultivating water body with wheel animalcule is that the wheel animalcule continuous culture system of the closed circulation of 1m3 is that example further specifies, the bait (diatom and yeast) that system adopts, and consumption is respectively 200 * 10
4Ind/mL and 0.3-0.4 gram/(10
6The wheel animalcule sky).Diatom is to be cultivated to cultivate in the bucket by little algae to provide, and it can satisfy the needs that wheel animalcule is cultivated fully.The 500L water body of can gathering from system every day, the wheel animalcule average yield of system is 160 * 10
6Ind/day, this output reaches the several months and big technical problem do not occur.
Because water is recycling, has reduced external contaminated water source, the cultivation of little algae is guaranteed, and the nutritional quality of wheel animalcule also can obtain stricter control.Simultaneity factor has been saved the amount of labour and production cost greatly by the water body of management much less.
More than show and described basic principle of the present invention and principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; that describes in the foregoing description and the specification just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications; these changes and improvements all fall in the claimed scope of the invention, and the claimed scope of the present invention is defined by appending claims and equivalent thereof.
Claims (9)
1, a kind of wheel animalcule continuous culture system of closed circulation, it is characterized in that it is used for wheel animalcule that wheel animalcule cultivates by at least one group and cultivates bucket, at least one group and be used for wheel animalcule recovering device, that wheel animalcule fortification bucket, that wheel animalcule carries out fortification is used to gather in the crops the wheel animalcule after the cultivation and comprise the water treatment facilities of physical filtering, biofiltration or disinfection etc. and at least one group and be used for specific little algae and cultivate and supply with described wheel animalcule and cultivate little algae of bucket and cultivate bucket and constitute; The output that described wheel animalcule is cultivated bucket is connected with described wheel animalcule fortification bucket, the output of described wheel animalcule fortification bucket is connected with described wheel animalcule recovering device, described wheel animalcule recovering device is connected with water treatment facilities, the output of described water treatment facilities is cultivated bucket with described little algae and is connected, and described little algae is cultivated bucket and cultivates bucket UNICOM with described wheel animalcule.
2, wheel animalcule continuous culture system as claimed in claim 1 is characterized in that, it is one group of chemostat that is used for the wheel animalcule cultivation that described wheel animalcule is cultivated bucket, cultivates in the bucket described wheel animalcule and is provided with aerator, and cultivating for wheel animalcule provides oxygen and realization immixture.
3, wheel animalcule continuous culture system as claimed in claim 2 is characterized in that, described wheel animalcule is cultivated bucket also can be provided with an adjustment control device that makes described wheel animalcule cultivation bucket maintenance water temperature as required.
4, wheel animalcule continuous culture system as claimed in claim 1 is characterized in that, can add nutrition fortifier in the described wheel animalcule fortification bucket wheel animalcule is carried out fortification.
5, wheel animalcule continuous culture system as claimed in claim 1 is characterized in that, described wheel animalcule recovering device adopts bolting silk or mesh to filter, and also can adopt rotation net purpose reaping machine.
6, wheel animalcule continuous culture system as claimed in claim 5 is characterized in that, the aperture of described mesh is the 80-100 micron.
7, wheel animalcule continuous culture system as claimed in claim 1 is characterized in that, described water treatment facilities comprises physical filtering, biofiltration or disinfection, and described physical filtering adopts sandfiltration pot, Microfilter or air supporting machine; Biofilter is adopted in biofiltration, and disinfection adopts ultraviolet sterilizer, ozone equipment or photo-catalyst device; Described water treatment facilities adopts one of above-mentioned or its combination.
8, wheel animalcule continuous culture system as claimed in claim 1, it is characterized in that, described little algae is cultivated the cultivation bucket that bucket is one group of little algae of post-directed training under the illumination effect, and described little algae is cultivated in the bucket and is provided with aerator, and cultivating for little algae provides carbonic acid gas and realize immixture.
9, wheel animalcule continuous culture system as claimed in claim 8, it is characterized in that, cultivate bucket in described little algae and be provided with micro algae culturing liquid, described micro algae culturing liquid adopts the F/2 prescription, little algae is cultivated finite concentration, and the algae liquid of then little algae being cultivated in the bucket is transported to the bait of cultivating as wheel animalcule in the described wheel animalcule cultivation bucket.
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| CN200910196065A CN101653106A (en) | 2009-09-22 | 2009-09-22 | Closed circulating rotifer continuous culturing system |
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| CN200910196065A CN101653106A (en) | 2009-09-22 | 2009-09-22 | Closed circulating rotifer continuous culturing system |
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| CN101653106A true CN101653106A (en) | 2010-02-24 |
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| CN200910196065A Pending CN101653106A (en) | 2009-09-22 | 2009-09-22 | Closed circulating rotifer continuous culturing system |
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Cited By (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102246722A (en) * | 2011-06-10 | 2011-11-23 | 浙江海洋学院 | High density rotifer culture device |
| CN102487903A (en) * | 2011-12-06 | 2012-06-13 | 中国水产科学研究院渔业机械仪器研究所 | Breeding device for planktonic animals |
| CN102599109A (en) * | 2012-02-29 | 2012-07-25 | 谢达祥 | Simple method for purifying rotifers in batch |
| CN102845367A (en) * | 2012-09-26 | 2013-01-02 | 环境保护部南京环境科学研究所 | Automation running-water type cultivation device of daphnia magna |
| CN103743877A (en) * | 2013-12-20 | 2014-04-23 | 常州市环境监测中心 | Dynamic exposure device for daphnia detection |
| CN103858815A (en) * | 2012-12-18 | 2014-06-18 | 上海市水产研究所 | Method for ecologically cultivating rotifers in summer eastern puffer fish fingerling culture pond |
| CN104255671A (en) * | 2014-09-26 | 2015-01-07 | 盐城工学院 | Branchionus plicatilis artificial water circulation high-yield breeding system and method |
| CN104719207A (en) * | 2015-03-24 | 2015-06-24 | 临沂大学 | Method for cultivating branchionus plicatilis through waste plastic buckets |
| CN105284742A (en) * | 2015-11-16 | 2016-02-03 | 中国水产科学研究院淡水渔业研究中心 | Rotifer continuous culturing system and rotifer continuous culturing method |
| CN105613433A (en) * | 2016-03-03 | 2016-06-01 | 武汉康立斯科技发展有限公司 | Method and system for culturing rotifer in freshwater |
| CN105707024A (en) * | 2016-03-03 | 2016-06-29 | 上海海洋大学 | Method for automatically collecting and culturing zooplankton |
| CN105794706A (en) * | 2014-12-31 | 2016-07-27 | 付顺林 | Ecological-type closed-water-recycling aquaculture system |
| CN106212392A (en) * | 2016-08-01 | 2016-12-14 | 中国科学院海洋研究所 | A kind of improve wheel animalcule growth and breeding ability and strengthening its as the method for food nutrition |
| CN107018961A (en) * | 2017-04-14 | 2017-08-08 | 中国科学院海洋研究所 | A kind of wheel animalcule automation high density culture system and method |
| CN107557290A (en) * | 2017-09-28 | 2018-01-09 | 复旦大学 | A kind of perseveranceization bactogen of microalgae |
| CN108719151A (en) * | 2018-05-15 | 2018-11-02 | 盘锦光合蟹业有限公司 | A kind of circulating outdoor earth pond wheel animalcule culture apparatus and method |
| CN110100773A (en) * | 2019-03-25 | 2019-08-09 | 福建省水产研究所（福建水产病害防治中心） | A kind of brachionus plicatilis quick propagation method |
| CN111248139A (en) * | 2020-03-27 | 2020-06-09 | 浙江省海洋水产养殖研究所 | Culture device and method for high-density rotifers |
| CN111528147A (en) * | 2020-05-21 | 2020-08-14 | 中国水产科学研究院东海水产研究所 | Small water body high-density rotifer cultivation method |
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2009
- 2009-09-22 CN CN200910196065A patent/CN101653106A/en active Pending
Cited By (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102246722B (en) * | 2011-06-10 | 2013-03-06 | 浙江海洋学院 | High density rotifer culture device |
| CN102246722A (en) * | 2011-06-10 | 2011-11-23 | 浙江海洋学院 | High density rotifer culture device |
| CN102487903A (en) * | 2011-12-06 | 2012-06-13 | 中国水产科学研究院渔业机械仪器研究所 | Breeding device for planktonic animals |
| CN102599109A (en) * | 2012-02-29 | 2012-07-25 | 谢达祥 | Simple method for purifying rotifers in batch |
| CN102845367A (en) * | 2012-09-26 | 2013-01-02 | 环境保护部南京环境科学研究所 | Automation running-water type cultivation device of daphnia magna |
| CN102845367B (en) * | 2012-09-26 | 2013-12-04 | 环境保护部南京环境科学研究所 | Automation running-water type cultivation device of daphnia magna |
| CN103858815B (en) * | 2012-12-18 | 2015-09-09 | 上海市水产研究所 | A kind of interior ecological method of cultivating wheel animalcule of Fugu fingerling culturing pool in summer |
| CN103858815A (en) * | 2012-12-18 | 2014-06-18 | 上海市水产研究所 | Method for ecologically cultivating rotifers in summer eastern puffer fish fingerling culture pond |
| CN103743877A (en) * | 2013-12-20 | 2014-04-23 | 常州市环境监测中心 | Dynamic exposure device for daphnia detection |
| CN103743877B (en) * | 2013-12-20 | 2015-09-16 | 常州市环境监测中心 | Magna detection dynamic exposing device |
| CN104255671A (en) * | 2014-09-26 | 2015-01-07 | 盐城工学院 | Branchionus plicatilis artificial water circulation high-yield breeding system and method |
| CN105794706A (en) * | 2014-12-31 | 2016-07-27 | 付顺林 | Ecological-type closed-water-recycling aquaculture system |
| CN104719207A (en) * | 2015-03-24 | 2015-06-24 | 临沂大学 | Method for cultivating branchionus plicatilis through waste plastic buckets |
| CN105284742A (en) * | 2015-11-16 | 2016-02-03 | 中国水产科学研究院淡水渔业研究中心 | Rotifer continuous culturing system and rotifer continuous culturing method |
| CN105707024B (en) * | 2016-03-03 | 2018-04-13 | 上海海洋大学 | A kind of method of automatic harvesting culture zooplankter |
| CN105613433A (en) * | 2016-03-03 | 2016-06-01 | 武汉康立斯科技发展有限公司 | Method and system for culturing rotifer in freshwater |
| CN105707024A (en) * | 2016-03-03 | 2016-06-29 | 上海海洋大学 | Method for automatically collecting and culturing zooplankton |
| CN105613433B (en) * | 2016-03-03 | 2018-10-02 | 武汉康立斯科技发展有限公司 | Freshwater rotifer breeding method and system |
| CN106212392A (en) * | 2016-08-01 | 2016-12-14 | 中国科学院海洋研究所 | A kind of improve wheel animalcule growth and breeding ability and strengthening its as the method for food nutrition |
| CN107018961A (en) * | 2017-04-14 | 2017-08-08 | 中国科学院海洋研究所 | A kind of wheel animalcule automation high density culture system and method |
| CN107018961B (en) * | 2017-04-14 | 2020-01-24 | 中国科学院海洋研究所 | Automatic high-density breeding system and method for rotifers |
| CN107557290A (en) * | 2017-09-28 | 2018-01-09 | 复旦大学 | A kind of perseveranceization bactogen of microalgae |
| CN108719151A (en) * | 2018-05-15 | 2018-11-02 | 盘锦光合蟹业有限公司 | A kind of circulating outdoor earth pond wheel animalcule culture apparatus and method |
| CN110100773A (en) * | 2019-03-25 | 2019-08-09 | 福建省水产研究所（福建水产病害防治中心） | A kind of brachionus plicatilis quick propagation method |
| CN110100773B (en) * | 2019-03-25 | 2022-01-25 | 福建省水产研究所（福建水产病害防治中心） | Rapid propagation method for Brachionus plicatilis |
| CN111248139A (en) * | 2020-03-27 | 2020-06-09 | 浙江省海洋水产养殖研究所 | Culture device and method for high-density rotifers |
| CN111528147A (en) * | 2020-05-21 | 2020-08-14 | 中国水产科学研究院东海水产研究所 | Small water body high-density rotifer cultivation method |
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Application publication date: 20100224 |