CN102550246A - Application method of photosynthetic bacteria culture solution in watermelon protected cultivation - Google Patents
Application method of photosynthetic bacteria culture solution in watermelon protected cultivation Download PDFInfo
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- CN102550246A CN102550246A CN2011104506432A CN201110450643A CN102550246A CN 102550246 A CN102550246 A CN 102550246A CN 2011104506432 A CN2011104506432 A CN 2011104506432A CN 201110450643 A CN201110450643 A CN 201110450643A CN 102550246 A CN102550246 A CN 102550246A
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- watermelon
- photosynthetic bacteria
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention provides an application method of a photosynthetic bacteria culture solution in watermelon protected cultivation. The application method comprises the following steps of: diluting the photosynthetic bacteria culture solution, and then, carrying out uniform seed soaking, root irrigation and spraying. According to the application method, the disease resistance, insect resistance and stress resistance of the watermelon in a sheltered ground can be improved, the photosynthetic intensity is enhanced, and the healthy growth of the watermelon is promoted; the soluble sugar content is increased, and the problem of quality decline arising from the use of chemical fertilizers and pesticides in artificial cultivation is solved; the residue of the pesticides and the chemical fertilizers and other toxic and hazardous substances in soil and plants are degraded, so that the safety of agricultural products is ensured; and the organic matter content in the soil can be effectively increased through the carbon and nitrogen fixation function of the watermelon, and therefore, the consumption of the chemical fertilizers is saved by 1/3. According to the application method, the application quantity of the pesticides and the chemical fertilizers in the watermelon protected cultivation is reduced, the residue of any hazardous substance cannot be caused, and therefore, the production demands of the watermelon on green and organic food standards can be met.
Description
Technical field
The invention belongs to the cultivating watermelon technical field, the application method of particularly a kind of photosynthetic bacteria culture fluid in watermelon protection ground cultivation.
Background technology
Watermelon has another name called water melon, cold melon, summer melon, is the fruit of cucurbitaceous plant watermelon, because of being introduces from the Western Regions in the Han dynasty, so claim " watermelon ".Melon is rounded or oval, color of the leather has dark green, green, white or green folder snake etc.It is dense red, light red, yellow or white that flesh is, and the sugariness succulence clearly quenches one's thirst, and is good fruit in midsummer.Watermelon can be rated as " king in the melon ", except that not fatty and cholesterol, contains significant quantities of glucose, malic acid, fructose, Argine Monohydrochloride, tomatin and abundant materials such as vitamin C, is a kind of food of being rich in nutrition, pure, edible safety.All there is cultivation China various places.Improving constantly of Along with people's living standard in recent years, watermelon also begins to adopt facilities such as booth, greenhouse to cultivate with protecting, and the year-round supply of watermelon is become a reality.
In the development of watermelon protection ground cultivation large tracts of land, the defective of its existence and deficiency also exposed day by day are come out.The one, owing to have a moderate climate in the green house, make insect be difficult to rely on arctic weather to be eliminated, add that indoor hot and humid weather very easily brings out the several diseases insect pest of watermelon, the conventional control method is all sprayed insecticide.Along with the drug-fast continuous enhancing of damage by disease and insect to conventional pesticide, the frequency of utilization of agricultural chemicals improves, and dosage strengthens, so that causes that the edible safety of watermelon more and more is a problem on the market.The 2nd, because the closed environment of protection facility, illumination in the facility plastic greenhouse, carbonic acid gas wretched insufficiency, the crop photosynthesis effect is suppressed, and has influenced growing of watermelon plant, thereby causes the dual reduction of The yield of watermelon and quality.The 3rd, in order to pursue unilateral economic benefit; One or two kind of crop such as watermelon only planted at the plantation family throughout the year in green house, think little of the crop rotation with other vegetable crop, and the soil germ is increased year by year; Soil-borne disease increases the weight of every year, has seriously restricted the development of watermelon protection ground cultivation.
At present, researchers apply bio-fertilizer on agricultural, and bio-fertilizer has nontoxic, improves the soil, characteristics free from environmental pollution.Utilize photosynthetic bacteria (Photosynthetic Bacteria; Abbreviation PSB) the photosynthetic bacteria culture fluid of producing promptly is a kind of bio-fertilizer; It is to be that raw material, active photosynthetic bacteria are bacterial classification with the autogamy medium, a kind of plyability bio-fertilizer that develops through modern biotechnology.Because it can promote plant growth, enhancing crop defending and fighting against diseases ability, degrading pesticide residues and fixing poisonous and harmful heavy metallic can promote soil substance to transform again, increase soil fertility, so crops are used its preparation, can receive the effect of increasing of quality and production.Through the existing documents and materials of retrieval, there is not public technology to relate to the report that the photosynthetic bacteria culture fluid is used as yet in watermelon protection ground cultivation.
Summary of the invention
The purpose of this invention is to provide the application method of a kind of photosynthetic bacteria culture fluid in watermelon protection ground cultivation, can satisfy the production needs of pollution-free food, organic food standards watermelon.
For realizing above-mentioned purpose, the technical scheme that the present invention adopts is following:
The application method of a kind of photosynthetic bacteria culture fluid in watermelon protection ground cultivation may further comprise the steps:
⑴ insert 1/10-1/5 photosynthetic bacteria seed culture fluid in the photosynthetic bacteria culture medium through sterilization, 15 ℃-20 ℃ of temperature; Intensity of illumination is that anaerobism was cultivated 3-10 days under the 800lux – 2000lux condition, and obtaining viable count is the photosynthetic bacteria culture fluid of 108-1010 cfu/ml;
⑵ be diluted with water to 20-100 times of original volume with the photosynthetic bacteria culture fluid, and seed soaking is 10-60 minutes before the watermelon sowing;
⑶ be diluted with water to 50-200 times of original volume with the photosynthetic bacteria culture fluid, final singling or transplant after, with the usage amount of every mu of watermelon l-10kg with the filling root that waters;
⑷ be diluted with water to 50-200 times of original volume with the photosynthetic bacteria culture fluid, evenly sprays vegetative period 1 time, expanding stage 2 times with the usage amount of every mu of each l-10kg of watermelon;
Photosynthetic bacteria is a Rhodospirillum in the described photosynthetic bacteria culture fluid
Rhodospirillium, Rhodopseudomonas
Rhodopseudomonas, red bacterium belongs to
RhodobacterIn any strain bacterium or their combination in any.
The photosynthetic bacteria culture medium of said inoculation photosynthetic bacteria, it consists of:
Sodium acetate 1000-2000mg CaCl2.2H2O 50-100 mg
MgSO4.7H2O 100-300 mg EDTA 10-30 mg
Yeast extract 500-1500 mg K2HPO4 500-1500 mg
(NH4)2SO4 1000-2000 mg KH2PO4 400-1000 mg
FeSO4. 7H2O 5-15mg trace element solution 1-5m1
Deionized water 1000-2000 ml pH 6-8
Wherein trace element solution consists of:
H3BO3 200-300 mg Na2MoO4.2H2O 50-100 mg
CuSO4 2-10 mg MnSO4. 4H2O 150-250 mg
ZnSO4.7H2O 20-30 mg deionized water 100-200 ml
Compared with prior art, the present invention has following beneficial effect:
The first, adopt the photosynthetic bacteria culture fluid to improve disease-resistant, pest-resistant, the degeneration-resistant border ability of protection ground watermelon, strengthen photosynthetic intensity, promote protection ground watermelon growing to grow, improve output;
The second, adopt the photosynthetic bacteria culture fluid to reduce the harmful components such as nitrate of protection ground watermelon, increase soluble sugar content, improve quality decline problem because of using chemical fertilizer and agricultural chemicals to cause;
The 3rd, adopt the photosynthetic bacteria culture fluid to improve the soil, residue of pesticide, heavy-metal residual and harmful microorganism ensure agricultural product security to the harm and the pollution of watermelon in the elimination soil;
The 4th, adopt the photosynthetic bacteria culture fluid to improve the rhizosphere nitrogenase activity, solid carbon, nitrogen fixation through himself effectively increase soil with organic matter content, save the fertilizer amount of l/3.
The 5th, photosynthetic bacteria culture fluid raw material sources of the present invention are wide, and low price can not cause any harmful substance residual, can satisfy the production needs of pollution-free food, organic food standards watermelon.
Embodiment
Below in conjunction with embodiment the present invention is made further specific descriptions:
Embodiment 1
Press following set of dispense system photosynthetic bacteria culture medium:
Sodium acetate 1000-2000mg CaCl2.2H2O 50-100 mg
MgSO4.7H2O 100-300 mg EDTA 10-30 mg
Yeast extract 500-1500 mg K2HPO4 500-1500 mg
(NH4)2SO4 1000-2000 mg KH2PO4 400-1000 mg
FeSO4. 7H2O 5-15mg trace element solution 1-5m1
Deionized water 1000-2000 ml pH 6-8
Wherein trace element solution consists of:
H3BO3 200-300 mg Na2MoO4.2H2O 50-100 mg
CuSO4 2-10 mg MnSO4. 4H2O 150-250 mg
ZnSO4.7H2O 20-30 mg deionized water 100-200 ml
In through the photosynthetic bacteria culture medium of sterilization, insert 1/10 photosynthetic bacteria seed culture fluid, 15 ℃-20 ℃ of temperature; Intensity of illumination is that anaerobism was cultivated 10 days under the 1800lux condition, and obtaining viable count is the photosynthetic bacteria culture fluid of 108-1010 cfu/ml.
In general 1 month of the middle and lower reach of Yangtze River area, carry out watermelon protection ground cultivation seedling-cultivating the last ten-days period, before the sowing except that common high-temperature seed presoaking, again with the photosynthetic bacteria culture fluid seed soaking that is diluted to 50 times of original volumes 30 minutes.After the final singling, the photosynthetic bacteria culture fluid is diluted with water to 200 times of original volume with the filling root of irrigating the fields, consumption is 6 kilograms/mu.The photosynthetic bacteria culture fluid is diluted with water to 150 times of original volume, and vegetative period 1 time, expanding stage 2 times evenly spray with the usage amount of every mu of each 7kg.Evidence, disease-resistant, pest-resistant, the degeneration-resistant border ability of watermelon obviously strengthens behind the use photosynthetic bacteria culture fluid, grows soon, and growing power is strong, and it is high to receive luminosity, compares according to plantation group output increase by 19.38%, and rate of fertilizer reduces by 1/3.
Embodiment 2
The preparation method of photosynthetic bacteria culture medium is with embodiment 1.
In through the photosynthetic bacteria culture medium of sterilization, insert 1/5 photosynthetic bacteria seed culture fluid, 15 ℃-20 ℃ of temperature; Intensity of illumination is that anaerobism was cultivated 8 days under the 1200lux condition, and obtaining viable count is the photosynthetic bacteria culture fluid of 108-1010 cfu/ml.
In general 1 month of the middle and lower reach of Yangtze River area, carry out watermelon protection ground cultivation seedling-cultivating the last ten-days period, before the sowing except that common high-temperature seed presoaking, again with the photosynthetic bacteria culture fluid seed soaking that is diluted to 100 times of original volumes 60 minutes.After the final singling, the photosynthetic bacteria culture fluid is diluted with water to 100 times of original volume with the filling root of irrigating the fields, consumption is 10 kilograms/mu.The photosynthetic bacteria culture fluid is diluted with water to 70 times of original volume, and vegetative period 1 time, expanding stage 2 times evenly spray with the usage amount of every mu of each 10kg.Evidence, disease-resistant, pest-resistant, the degeneration-resistant border ability of watermelon obviously strengthens behind the use photosynthetic bacteria culture fluid, grows soon, and growing power is strong, and it is high to receive luminosity, compares according to plantation group output increase by 20.24%, and rate of fertilizer reduces by 1/3.
Claims (2)
1. a photosynthetic bacteria culture fluid is protected the application method in the ground cultivation at watermelon, it is characterized in that may further comprise the steps:
⑴ insert 1/10-1/5 photosynthetic bacteria seed culture fluid in the photosynthetic bacteria culture medium through sterilization, 15 ℃-20 ℃ of temperature; Intensity of illumination is that anaerobism was cultivated 3-10 days under the 800lux – 2000lux condition, and obtaining viable count is the photosynthetic bacteria culture fluid of 108-1010 cfu/ml;
⑵ be diluted with water to 20-100 times of original volume with the photosynthetic bacteria culture fluid, and seed soaking is 10-60 minutes before the watermelon sowing;
⑶ be diluted with water to 50-200 times of original volume with the photosynthetic bacteria culture fluid, final singling or transplant after, with the usage amount of every mu of watermelon l-10kg with the filling root that waters;
⑷ be diluted with water to 50-200 times of original volume with the photosynthetic bacteria culture fluid, evenly sprays vegetative period 1 time, expanding stage 2 times with the usage amount of every mu of each l-10kg of watermelon.
2. the application method of photosynthetic bacteria culture fluid according to claim 1 in watermelon protection ground cultivation, it is characterized in that: photosynthetic bacteria is a Rhodospirillum in the described photosynthetic bacteria culture fluid
Rhodospirillium, Rhodopseudomonas
Rhodopseudomonas, red bacterium belongs to
RhodobacterIn any strain bacterium or their combination in any.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2011104506432A CN102550246A (en) | 2011-12-29 | 2011-12-29 | Application method of photosynthetic bacteria culture solution in watermelon protected cultivation |
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| CN2011104506432A CN102550246A (en) | 2011-12-29 | 2011-12-29 | Application method of photosynthetic bacteria culture solution in watermelon protected cultivation |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102992894A (en) * | 2012-11-20 | 2013-03-27 | 蒋常德 | Preparation method for high-concentration photosynthesis bacterial manure |
| CN103283611A (en) * | 2013-06-13 | 2013-09-11 | 郑洪振 | Automatic pet toilet |
| CN103340086A (en) * | 2013-07-24 | 2013-10-09 | 太仓市临江农场专业合作社 | Method for planting watermelons |
| CN106754468A (en) * | 2016-11-15 | 2017-05-31 | 沈阳师范大学 | Microbial bacterial agent and preparation for leaf vegetables culture, application process |
-
2011
- 2011-12-29 CN CN2011104506432A patent/CN102550246A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102992894A (en) * | 2012-11-20 | 2013-03-27 | 蒋常德 | Preparation method for high-concentration photosynthesis bacterial manure |
| CN102992894B (en) * | 2012-11-20 | 2014-08-06 | 北京市波普克生态科技有限公司 | Preparation method for high-concentration photosynthesis bacterial manure |
| CN103283611A (en) * | 2013-06-13 | 2013-09-11 | 郑洪振 | Automatic pet toilet |
| CN103340086A (en) * | 2013-07-24 | 2013-10-09 | 太仓市临江农场专业合作社 | Method for planting watermelons |
| CN106754468A (en) * | 2016-11-15 | 2017-05-31 | 沈阳师范大学 | Microbial bacterial agent and preparation for leaf vegetables culture, application process |
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Application publication date: 20120711 |