CN103141293B - Scale planting method for increasing survival rate of saussurea obvallata - Google Patents
Scale planting method for increasing survival rate of saussurea obvallata Download PDFInfo
- Publication number
- CN103141293B CN103141293B CN201310112965.5A CN201310112965A CN103141293B CN 103141293 B CN103141293 B CN 103141293B CN 201310112965 A CN201310112965 A CN 201310112965A CN 103141293 B CN103141293 B CN 103141293B
- Authority
- CN
- China
- Prior art keywords
- seedling
- edgew
- saus
- soil
- seed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Cultivation Of Plants (AREA)
Abstract
The invention belongs to the field of manual reproduction of saussurea obvallata, and discloses a scale planting method for increasing the survival rate of saussurea obvallata. The method comprises the following steps of: selecting seeds; preserving heat for seeds to accelerate germination and grow seedlings; growing seedlings through tissue culture; and performing field soil transplanting and planting management. The method has the beneficial effects that 1, seed nutrient seedling growing and tissue seedling growing cooperate with each other, so that higher safety is achieved in comparison to a single way for growing seedlings, limitations on the quantity and seasons of single seed seedling growing are prevented, the defects of ageing and original plants and incapability of storing robust plants for a long time caused by single adoption of tissue seedling growing are overcome, and the survival rate and survival quantity of saussurea obvallata are increased greatly; and 2, a nutritional substrate contains alpine meadow soil, so that the nutritional demand and growing environment for seedling growth obtained through seed seedling growing and tissue seedling growing of the saussurea obvallata are realized, the seedling survival rate of the saussurea obvallata is increased, and field planting transition is performed for seedlings.
Description
Technical field
The invention belongs to the artificial propagation field of Saus-surea obvallata (DC.) Edgew.
Background technology
Saus-surea obvallata (DC.) Edgew (formal name used at school: Saussureaobvallata) be the plant of composite family Genus Saussurea.Perennial root draft.High 30-50cm.Leaf Long Circle or ellipse; Topmost leaf bract shape, yellow, membranous, capitulum 4-5 is wrapped in wherein; Plant tool overpowering odor.The florescence 8-9 month.Like cold wetting, happiness light, barren-resistant.Produce the west and south to the southeast, be born in the Alpine screes of height above sea level 3700-4800m more.Also produce southwest and Qinghai and Himalayas.Tibetan medicine uses this kind for saussurea involucrata, all herbal medicine, cures mainly rheumatic arthritis, high mountain and do not accommodate the diseases such as irregular menstruation.At present not yet by artificial introducing and planting.
Due to awful weather and very harsh geographical growing environment, natural propagation rate is low, poor growth, and wild lanatechead saussurea herb with flower is very rare in addition, and the destruction illegal mining of people to Saus-surea obvallata (DC.) Edgew causes wild Saus-surea obvallata (DC.) Edgew data endangered.From artificial culture lanatechead saussurea herb with flower, not only solve the problem of wild Saus-surea obvallata (DC.) Edgew resource, can also promote local people's economic life level, therefore, artificial breeding Saus-surea obvallata (DC.) Edgew is significant.
In Chinese patent application, disclose the implantation methods to saussurea involucrata, as number of patent application 03108092 discloses a kind of " hiding the implantation methods of saussurea involucrata ", number of patent application 201110454465 discloses a kind of " Xinjiang Saussurea involucrate scaled artificial planting method ".These two kinds of methods are all to transplant to field after the direct cultivation of saussurea involucrata seed is rooted.The drawback of this method is that nutrient matrix used is not too applicable to saussurea involucrata growth of seedling, so its seed bud ratio is low, and the survival rate of saussurea involucrata seedling waste seed, cost of labor is high, and its production cost is high.
The patent No. 200610092920 discloses a kind of " method of saussurea involucrata embryo forming seedling through one step culture ", and it is after saussurea involucrata seed sprouting, after subculture is repeatedly cultivated, to obtain saussurea involucrata seedling.This method subculture number is many, complex procedures.
Summary of the invention
The problem to be solved in the present invention is exactly a kind of method that provides seed seedling-raising and tissue to grow seedlings synergistic raising Saus-surea obvallata (DC.) Edgew seedling percent for above deficiency.Its technical scheme is as follows:
A scale implantation methods that improves Saus-surea obvallata (DC.) Edgew seedling percent, comprises the following steps:
1) seed is selected: screening Saus-surea obvallata (DC.) Edgew seed;
2) seed insulation Pregermination and seedling breeding: in annual March to April, first with the warm water of 15-40 ℃, plant growth regulator is diluted to growth regulating agent solution, with growth regulating agent solution, soak Saus-surea obvallata (DC.) Edgew seed, after 6-8 hour, pull out and dry, then use powerful root-inducing powder to be diluted to the aqueous solution, soak Saus-surea obvallata (DC.) Edgew seed 1-2 minute, again by Saus-surea obvallata (DC.) Edgew planting seed in nutrient matrix, sowing temperature 15-25 ℃, soil moisture 55-60%, sow and emerge for 6-9 days, in nutrient matrix, grow and within 60-90 days, become seedling;
3) tissue cultivating and seedling:
1. select robust plant, the Saus-surea obvallata (DC.) Edgew seedling growing 60-90 days, cleans up with circulating water;
2. the seedling of cleaning or stem or blade are carried out to surface sterilizing second with 75% alcohol-pickled 10-20;
3. the mercuric chloride solution that the seedling of sterilizing or stem or blade is placed in to 0.05-0.2% soaks 4-5 minute, then uses distilled water rinsing 4-5 time, uses aseptic filter paper suck dry moisture;
4. by sterile scissors, seedling or stem or blade are cut into 0.3-0.5cm
2the bulk of size is inoculated into this bulk in evoked callus medium and cultivates, each culture medium inoculated 3-4 piece under aseptic condition;
5. cultivate: the evoked callus medium of inoculating is placed in 20-25 ℃, the darkroom of humidity 55-60% and is cultivated after 5-7 days, again at illumination every day 8-10 hour, under the environment of intensity of illumination 70-90umol/m2.s, cultivate and induce the formation of root, bud in 20-30 days, and grow into Saus-surea obvallata (DC.) Edgew plantlet;
6. be transplanted in nutrient matrix:
Go out to have root to have the Saus-surea obvallata (DC.) Edgew plantlet of bud to be transplanted in nutrient matrix induction incubation growth, cover plastic film, 80-100 days grows seedlings in nutrient matrix;
4) field soil transplant survival:
Before transplanting, transplant field soil is ploughed deeply to thin ploughing, apply the fertilizer of 1000-2000Kg/ mu, plough under in soil to smooth, then the seedling of cultivating is piled to ridge cultivation, the high 20-25 centimetre in ridge, row spacing 25-30 centimetre, spacing in the rows 25-30 centimetre;
5) Cultivate administration: after Saus-surea obvallata (DC.) Edgew is transplanted, control the moisture 50%-60% of soil, soil drought is wanted timely moisturizing; After 70 days after transplanting, apply phosphoric acid calcareous fertilisers, consumption is 20kg/ mu, sprays 0.15% potassium dihydrogen phosphate, promotes the growth of Saus-surea obvallata (DC.) Edgew.
Compared with prior art, the beneficial effect that the present invention has is:
1, use the method to obtain Saus-surea obvallata (DC.) Edgew seedling by amount reproduction, the plant breeding of seedling primary growth stalwartness is expanded, reduce seed usage amount, shorten seed sprouting growth time, keep the stable of tree characteristics.
2, in nutrient matrix, contain alpine meadow soil, meet Saus-surea obvallata (DC.) Edgew by seed seedling-raising and nutritional need and the growing environment of organizing the growth of seedling of the acquisition of growing seedlings, improved the seedling percent of Saus-surea obvallata (DC.) Edgew, for seedling carries out field planting transition.
3, use method for tissue culture greatly to expand the growth population of Saus-surea obvallata (DC.) Edgew, improved output.Because be not to develop into plant with seed sprouting in tissue is cultivated, but become plant by cell development, yet the quantity of cell is very many, so improved the output of growing seedlings.
The synergy of 4, growing seedlings by seed nutrition pot grown seedling and tissue, than adopting separately a kind of mode to grow seedlings, more insure, prevent that single seed seedling-raising is in quantity and seasonal restriction, overcome primordial plant aging that single employing tissue grows seedlings brought and can not preserve for a long time the shortcoming of healthy and strong plant, greatly improve the survival rate of Saus-surea obvallata (DC.) Edgew and survive quantity.
In order to prove the impact of nutrient matrix formula of the present invention on Saus-surea obvallata (DC.) Edgew seedling percent, test below special work:
Formula 1: 60 parts of humus soils, 40 parts of fertilizers;
Formula 2: 60 parts of humus soils, 20 parts of fine sands, 20 parts of fertilizers;
Formula 3: 60 parts of humus soils, 20 parts, alpine meadow soil, 20 parts of fertilizers;
Formula 4: 60 parts of humus soils, 20 parts of fine sands, 16 parts of fertilizers, 1.5 parts, ammonium nitrate, 0.1 part of potassium dihydrogen phosphate;
Formula 5: 50 parts of humus soils, 20 parts of fine sands, 16 parts of fertilizers, 10 parts, alpine meadow soil, 1 part, ammonium nitrate, 0.1 part of potassium dihydrogen phosphate;
Formula 6: 50 parts of humus soils, 15 parts of fine sands, 16 parts of fertilizers, 10 parts, alpine meadow soil, 1 part, ammonium nitrate, 0.1 part of potassium dihydrogen phosphate, 5 parts, nitrogenous fertilizer.
Test divides two groups to be carried out, and selects 600 full Saus-surea obvallata (DC.) Edgew seeds to put into different nutrient matrix formulas for every group and cultivates a seedling, and to record the germination rate of Saus-surea obvallata (DC.) Edgew seed, growing state is index, the formula of selected nutrient matrix.Result of the test is as follows
Different Nutrition matrix formulations affects situation to the germinating growth of Saus-surea obvallata (DC.) Edgew
From result of the test, draw, select formula 6 the nutrient matrix formula Saus-surea obvallata (DC.) Edgew seed of growing seedlings, germination rate is high, and late growing stage is healthy and strong, produces fast.
With the inventive method transplanting and management planting effect:
Adopt the inventive method to transplant region to three tests and carried out Saus-surea obvallata (DC.) Edgew survival rate statistics
From this, the inventive method has remarkable result to improving Saus-surea obvallata (DC.) Edgew seedling percent, is suitable for implant mass.
Embodiment
Embodiment mono-:
A scale implantation methods that improves Saus-surea obvallata (DC.) Edgew seedling percent, comprises the following steps:
1) seed is selected: screening full grains, brownish black, individual greatly with the particle of gloss as Saus-surea obvallata (DC.) Edgew seed;
2) seed insulation Pregermination and seedling breeding: to April, first with the warm water of 15 ℃, plant growth regulator triacontanol being diluted to mass concentration is 0.1*10 in annual March
-16growth regulating agent solution, with growth regulating agent solution, soak Saus-surea obvallata (DC.) Edgew seed (solution flooded Saus-surea obvallata (DC.) Edgew seed 2-5cm is advisable), after 8 hours, pull out and dry, then use powerful root-inducing powder in mass ratio 1:1500 be diluted to the aqueous solution, soak Saus-surea obvallata (DC.) Edgew seed 1 minute, again by Saus-surea obvallata (DC.) Edgew planting seed in nutrient matrix, cover 2 cm thick soil, 25 ℃ of sowing temperature, soil moisture 55%, sow and emerge for 9 days, in nutrient matrix, grow and within 60 days, become seedling;
3) tissue cultivating and seedling:
1. select robust plant, the Saus-surea obvallata (DC.) Edgew seedling growing 60 days (also can select stem or the unaged blade of the fresh Saus-surea obvallata (DC.) Edgew of field acquisition), cleans up with circulating water;
2. by 75% the surface sterilizing that carries out for alcohol-pickled 10 seconds for the seedling of cleaning or stem or blade;
3. the seedling of sterilizing or stem or blade are placed in to 0.05% mercuric chloride solution and soak 5 minutes, in immersion process, stir gently seedling plant, make surface fully touch mercuric chloride solution, then use distilled water rinsing 4 times, use aseptic filter paper suck dry moisture;
4. by sterile scissors, seedling or stem or blade are cut into 0.3-0.5cm
2the bulk of size is inoculated into this bulk in evoked callus medium and cultivates, each culture medium inoculated 3-4 piece under aseptic condition;
5. cultivate: the evoked callus medium of inoculating is placed in 20 ℃, the darkroom of humidity 60% and is cultivated after 5 days, again illumination every day 10 hours, under the environment of intensity of illumination 70umol/m2.s, cultivate and induce the formation of root, bud in 30 days, and grow into Saus-surea obvallata (DC.) Edgew plantlet;
6. be transplanted in nutrient matrix:
Go out to have root to have the Saus-surea obvallata (DC.) Edgew plantlet of bud to be transplanted in the nutrient matrix with soil property induction incubation growth, after transplanting, cover temperature, the humidity of plastic film to keep plant strain growth environment to need, to improve transplanting survival rate, then in nutrient matrix, grow seedlings 80 days;
4) field soil transplant survival:
Select height above sea level more than 1800 meters, day and night temperature is large, and rainfall is plentiful, the place that has accumulated snow winter, and draining is good, is rich in humus, the band sandy soil earth of loosing soil, as transplant field soil, before transplanting, is ploughed deeply thin ploughing to transplant field soil, apply the fertilizer (being farmyard manure) of 1000Kg/ mu, plough under in soil to smooth, then the seedling of cultivating is piled to ridge cultivation, ridge is high 25 centimetres, and 25 centimetres of row spacings, during transplanting, root is implanted together with nutrient matrix between transplant field to 30 centimetres of spacing in the rows;
5) Cultivate administration: after Saus-surea obvallata (DC.) Edgew is transplanted, control the moisture 50% of soil, soil drought is wanted timely moisturizing; Growing period, palpus weeding; After 70 days after transplanting, apply phosphoric acid calcareous fertilisers, consumption is 20kg/ mu, sprays 0.15% potassium dihydrogen phosphate, promotes the growth of Saus-surea obvallata (DC.) Edgew.
Described nutrient matrix comprises the raw material of following weight portion:
50 parts of humus soils, 15 parts of fine sands, 16 parts of fertilizers (being farmyard manure), alpine meadow soil (manually gathers from alpine meadow, take the soil layer of 8-15 centimeters under grassy marshland) 15 parts, after mixing, remove lump and impurity, 1 part, ammonium nitrate, 0.2 part of potassium dihydrogen phosphate, 5 parts, nitrogenous fertilizer.
The preparation method of described nutrient matrix is: get 50 parts of humus soils, 15 parts of fine sands, 16 parts of fertilizers, 15 parts, alpine meadow soil, add 1 part, ammonium nitrate, 0.2 part of potassium dihydrogen phosphate, 5 parts, nitrogenous fertilizer, mix, adjust the pH value 5.5 of nutrient matrix, keep the moisture 55% of nutrient matrix.
The preparation method of described evoked callus medium is: get 0.6 part of 50 parts of sucrose, 15 parts, agar, 0.2 part of a-methyl α-naphthyl acetate, 6-benzyl aminopurine, 2,0.3 part of 4-dichlorphenoxyacetic acid, 0.4 part of mixing of heteroauxin, adjust pH 5.0, after being heated to dissolve, divide in 250 milliliters of triangular flasks that install to sterilizing standby.
Embodiment bis-:
A scale implantation methods that improves Saus-surea obvallata (DC.) Edgew seedling percent, comprises the following steps:
1) seed is selected: screening full grains, brownish black, individual greatly with the particle of gloss as Saus-surea obvallata (DC.) Edgew seed;
2) seed insulation Pregermination and seedling breeding: to April, first with the warm water of 40 ℃, plant growth regulator triacontanol being diluted to mass concentration is 0.1*10 in annual March
-16growth regulating agent solution, with growth regulating agent solution, soak Saus-surea obvallata (DC.) Edgew seed (solution flooded Saus-surea obvallata (DC.) Edgew seed 2-5cm is advisable), after 6 hours, pull out and dry, then use powerful root-inducing powder in mass ratio 1:1500 be diluted to the aqueous solution, soak Saus-surea obvallata (DC.) Edgew seed 2 minutes, again by Saus-surea obvallata (DC.) Edgew planting seed in nutrient matrix, cover 2 cm thick soil, 15 ℃ of sowing temperature, soil moisture 60%, sow and emerge for 6 days, in nutrient matrix, grow and within 90 days, become seedling;
3) tissue cultivating and seedling:
1. select robust plant, the Saus-surea obvallata (DC.) Edgew seedling growing 90 days (also can select stem or the unaged blade of the fresh Saus-surea obvallata (DC.) Edgew of field acquisition), cleans up with circulating water;
2. by 75% the surface sterilizing that carries out for alcohol-pickled 20 seconds for the seedling of cleaning or stem or blade;
3. the seedling of sterilizing or stem or blade are placed in to 0.2% mercuric chloride solution and soak 4 minutes, in immersion process, stir gently seedling plant, make surface fully touch mercuric chloride solution, then use distilled water rinsing 5 times, use aseptic filter paper suck dry moisture;
4. by sterile scissors, seedling or stem or blade are cut into 0.3-0.5cm
2the bulk of size is inoculated into this bulk in evoked callus medium and cultivates, each culture medium inoculated 3-4 piece under aseptic condition;
5. cultivate: the evoked callus medium of inoculating is placed in 25 ℃, the darkroom of humidity 55% and is cultivated after 7 days, again illumination every day 8 hours, under the environment of intensity of illumination 90umol/m2.s, cultivate and induce the formation of root, bud in 20 days, and grow into Saus-surea obvallata (DC.) Edgew plantlet;
6. be transplanted in nutrient matrix:
Go out to have root to have the Saus-surea obvallata (DC.) Edgew plantlet of bud to be transplanted in the nutrient matrix with soil property induction incubation growth, after transplanting, cover temperature, the humidity of plastic film to keep plant strain growth environment to need, to improve transplanting survival rate, then in nutrient matrix, grow seedlings 100 days;
4) field soil transplant survival:
Select height above sea level more than 1800 meters, day and night temperature is large, and rainfall is plentiful, the place that has accumulated snow winter, and draining is good, is rich in humus, the band sandy soil earth of loosing soil, as transplant field soil, before transplanting, is ploughed deeply thin ploughing to transplant field soil, apply the fertilizer (being farmyard manure) of 2000Kg/ mu, plough under in soil to smooth, then the seedling of cultivating is piled to ridge cultivation, ridge is high 20 centimetres, and 30 centimetres of row spacings, during transplanting, root is implanted together with nutrient matrix between transplant field to 25 centimetres of spacing in the rows;
5) Cultivate administration: after Saus-surea obvallata (DC.) Edgew is transplanted, control the moisture 60% of soil, soil drought is wanted timely moisturizing; Growing period, palpus weeding; After 70 days after transplanting, apply phosphoric acid calcareous fertilisers, consumption is 20kg/ mu, sprays 0.15% potassium dihydrogen phosphate, promotes the growth of Saus-surea obvallata (DC.) Edgew.
Described nutrient matrix comprises the raw material of following weight portion: 60 parts of humus soils, 10 parts of fine sands, 20 parts of fertilizers (being farmyard manure), alpine meadow soil (manually gathers from alpine meadow, take the soil layer of 8-15 centimeters under grassy marshland) 10 parts, after mixing, remove lump and impurity, 2 parts, ammonium nitrate, 0.1 part of potassium dihydrogen phosphate, 8 parts, nitrogenous fertilizer.
The preparation method of described nutrient matrix is: get 60 parts of humus soils, 10 parts of fine sands, 20 parts of fertilizers, 10 parts, alpine meadow soil, add 2 parts, ammonium nitrate, 0.1 part of potassium dihydrogen phosphate, 8 parts, nitrogenous fertilizer, mix, adjust the pH value 7 of nutrient matrix, keep the moisture 60% of nutrient matrix.
The preparation method of described evoked callus medium is: get 0.5 part of 60 parts of sucrose, 10 parts, agar, 0.4 part of a-methyl α-naphthyl acetate, 6-benzyl aminopurine, 2,0.4 part of 4-dichlorphenoxyacetic acid, 0.2 part of mixing of heteroauxin, adjust pH 7.0, after being heated to dissolve, divide in 250 milliliters of triangular flasks that install to sterilizing standby.
Embodiment tri-:
A scale implantation methods that improves Saus-surea obvallata (DC.) Edgew seedling percent, comprises the following steps:
1) seed is selected: screening full grains, brownish black, individual greatly with the particle of gloss as Saus-surea obvallata (DC.) Edgew seed;
2) seed insulation Pregermination and seedling breeding: to April, first with the warm water of 28 ℃, plant growth regulator triacontanol being diluted to mass concentration is 0.1*10 in annual March
-16growth regulating agent solution, with growth regulating agent solution, soak Saus-surea obvallata (DC.) Edgew seed (solution flooded Saus-surea obvallata (DC.) Edgew seed 2-5cm is advisable), after 7 hours, pull out and dry, then use powerful root-inducing powder in mass ratio 1:1500 be diluted to the aqueous solution, soak Saus-surea obvallata (DC.) Edgew seed 1.5 minutes, again by Saus-surea obvallata (DC.) Edgew planting seed in nutrient matrix, cover 2 cm thick soil, 20 ℃ of sowing temperature, soil moisture 57%, sow and emerge for 8 days, in nutrient matrix, grow and within 75 days, become seedling;
3) tissue cultivating and seedling:
1. select robust plant, the Saus-surea obvallata (DC.) Edgew seedling growing 75 days (also can select stem or the unaged blade of the fresh Saus-surea obvallata (DC.) Edgew of field acquisition), cleans up with circulating water;
2. by 75% the surface sterilizing that carries out for alcohol-pickled 15 seconds for the seedling of cleaning or stem or blade;
3. the seedling of sterilizing or stem or blade are placed in to 0.1% mercuric chloride solution and soak 4-5 minute, in immersion process, stir gently seedling plant, make surface fully touch mercuric chloride solution, then use distilled water rinsing 4-5 time, use aseptic filter paper suck dry moisture;
4. by sterile scissors, seedling or stem or blade are cut into 0.3-0.5cm
2the bulk of size is inoculated into this bulk in evoked callus medium and cultivates, each culture medium inoculated 3-4 piece under aseptic condition;
5. cultivate: the evoked callus medium of inoculating is placed in 22 ℃, the darkroom of humidity 57% and is cultivated after 6 days, again illumination every day 9 hours, under the environment of intensity of illumination 80umol/m2.s, cultivate and induce the formation of root, bud in 25 days, and grow into Saus-surea obvallata (DC.) Edgew plantlet;
6. be transplanted in nutrient matrix:
Go out to have root to have the Saus-surea obvallata (DC.) Edgew plantlet of bud to be transplanted in the nutrient matrix with soil property induction incubation growth, after transplanting, cover temperature, the humidity of plastic film to keep plant strain growth environment to need, to improve transplanting survival rate, then in nutrient matrix, grow seedlings 90 days;
4) field soil transplant survival:
Select height above sea level more than 1800 meters, day and night temperature is large, and rainfall is plentiful, the place that has accumulated snow winter, and draining is good, is rich in humus, the band sandy soil earth of loosing soil, as transplant field soil, before transplanting, is ploughed deeply thin ploughing to transplant field soil, apply the fertilizer (being farmyard manure) of 1500Kg/ mu, plough under in soil to smooth, then the seedling of cultivating is piled to ridge cultivation, ridge is high 22 centimetres, and 28 centimetres of row spacings, during transplanting, root is implanted together with nutrient matrix between transplant field to 28 centimetres of spacing in the rows;
5) Cultivate administration: after Saus-surea obvallata (DC.) Edgew is transplanted, control the moisture 55% of soil, soil drought is wanted timely moisturizing; Growing period, palpus weeding; After 70 days after transplanting, apply phosphoric acid calcareous fertilisers, consumption is 20kg/ mu, sprays 0.15% potassium dihydrogen phosphate, promotes the growth of Saus-surea obvallata (DC.) Edgew.
Described nutrient matrix comprises the raw material of following weight portion:
55 parts of humus soils, 12 parts of fine sands, 18 parts of fertilizers (being farmyard manure), alpine meadow soil (manually gathers from alpine meadow, take the soil layer of 8-15 centimeters under grassy marshland) 12 parts, after mixing, remove lump and impurity, 1.5 parts, ammonium nitrate, 0.15 part of potassium dihydrogen phosphate, 7 parts, nitrogenous fertilizer.
The preparation method of described nutrient matrix is: get 55 parts of humus soils, 12 parts of fine sands, 18 parts of fertilizers, 12 parts, alpine meadow soil, add 1.5 parts, ammonium nitrate, 0.15 part of potassium dihydrogen phosphate, 7 parts, nitrogenous fertilizer, mix, adjust the pH value 6 of nutrient matrix, keep the moisture 57% of nutrient matrix.
The preparation method of described evoked callus medium is: get 0.55 part of 55 parts of sucrose, 12 parts, agar, 0.3 part of a-methyl α-naphthyl acetate, 6-benzyl aminopurine, 2,0.35 part of 4-dichlorphenoxyacetic acid, 0.3 part of mixing of heteroauxin, adjust pH 6, after being heated to dissolve, divide in 250 milliliters of triangular flasks that install to sterilizing standby.
Claims (2)
1. a scale implantation methods that improves Saus-surea obvallata (DC.) Edgew seedling percent, is characterized in that comprising the following steps:
1) seed is selected: screening Saus-surea obvallata (DC.) Edgew seed;
2) seed insulation Pregermination and seedling breeding: in annual March to April, first with the warm water of 15-40 ℃, plant growth regulator is diluted to growth regulating agent solution, with growth regulating agent solution, soak Saus-surea obvallata (DC.) Edgew seed, after 6-8 hour, pull out and dry, then use powerful root-inducing powder to be diluted to the aqueous solution, soak Saus-surea obvallata (DC.) Edgew seed 1-2 minute, again by Saus-surea obvallata (DC.) Edgew planting seed in nutrient matrix, sowing temperature 15-25 ℃, soil moisture 55-60%, sow and emerge for 6-9 days, in nutrient matrix, grow and within 60-90 days, become seedling;
3) tissue cultivating and seedling:
1. select robust plant, the Saus-surea obvallata (DC.) Edgew seedling growing 60-90 days, cleans up with circulating water;
2. the seedling of cleaning or stem or blade are carried out to surface sterilizing second with 75% alcohol-pickled 10-20;
3. the mercuric chloride solution that the seedling of sterilizing or stem or blade is placed in to 0.05-0.2% soaks 4-5 minute, then uses distilled water rinsing 4-5 time, uses aseptic filter paper suck dry moisture;
4. by sterile scissors, seedling or stem or blade are cut into the bulk of 0.3-0.5cm2 size, under aseptic condition, this bulk are inoculated in evoked callus medium and are cultivated, each culture medium inoculated 3-4 piece;
5. cultivate: the evoked callus medium of inoculating is placed in 20-25 ℃, the darkroom of humidity 55-60% and is cultivated after 5-7 days, then at illumination every day 8-10 hour, intensity of illumination 70-90 μ mol/m
2under the environment of s, cultivate and induce the formation of root, bud in 20-30 days, and grow into Saus-surea obvallata (DC.) Edgew plantlet;
6. be transplanted in nutrient matrix:
Go out to have root to have the Saus-surea obvallata (DC.) Edgew plantlet of bud to be transplanted in nutrient matrix induction incubation growth, cover plastic film, 80-100 days grows seedlings in nutrient matrix;
Nutrient matrix comprises the raw material of following weight portion:
Humus soil 50-60 part, fine sand 10-15 part, fertilizer 16-20 part, alpine meadow soil 10-15 part, ammonium nitrate 1-2 part, potassium dihydrogen phosphate 0.1-0.2 part, nitrogenous fertilizer 5-8 part;
4) field soil transplant survival:
Before transplanting, transplant field soil is ploughed deeply to thin ploughing, apply the fertilizer of 1000-2000Kg/ mu, plough under in soil to smooth, then the seedling of cultivating is piled to ridge cultivation, the high 20-25 centimetre in ridge, row spacing 25-30 centimetre, spacing in the rows 25-30 centimetre;
5) Cultivate administration: after Saus-surea obvallata (DC.) Edgew is transplanted, control the moisture 50%-60% of soil, soil drought is wanted timely moisturizing; After 70 days after transplanting, apply phosphoric acid calcareous fertilisers, consumption is 20kg/ mu, sprays 0.15% potassium dihydrogen phosphate, promotes the growth of Saus-surea obvallata (DC.) Edgew.
2. improve according to claim 1 the scale implantation methods of Saus-surea obvallata (DC.) Edgew seedling percent, the preparation method who it is characterized in that nutrient matrix is: get humus soil 50-60 part, fine sand 10-15 part, fertilizer 16-20 part, alpine meadow soil 10-15 part, adds ammonium nitrate 1-2 part, potassium dihydrogen phosphate 0.1-0.2 part, nitrogenous fertilizer 5-8 part, mix, adjust the pH value 7-5.5 of nutrient matrix, keep the moisture 55-60% of nutrient matrix.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310112965.5A CN103141293B (en) | 2013-03-24 | 2013-04-02 | Scale planting method for increasing survival rate of saussurea obvallata |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310108076.1 | 2013-03-24 | ||
| CN201310108076 | 2013-03-24 | ||
| CN201310112965.5A CN103141293B (en) | 2013-03-24 | 2013-04-02 | Scale planting method for increasing survival rate of saussurea obvallata |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103141293A CN103141293A (en) | 2013-06-12 |
| CN103141293B true CN103141293B (en) | 2014-03-26 |
Family
ID=48539855
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310112965.5A Active CN103141293B (en) | 2013-03-24 | 2013-04-02 | Scale planting method for increasing survival rate of saussurea obvallata |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103141293B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105993557A (en) * | 2016-06-27 | 2016-10-12 | 安徽梅兰园林景观工程有限公司 | Planting technology of lotus flowers |
| CN114766339B (en) * | 2022-04-28 | 2023-05-26 | 西藏自治区农牧科学院蔬菜研究所 | Cultivation method of Tibetan meridian |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1907000A (en) * | 2005-08-01 | 2007-02-07 | 中国科学院过程工程研究所 | Scaled artificial planting method of saussurea involucrate tissue culture sprout |
| CN100337534C (en) * | 2005-08-29 | 2007-09-19 | 中国科学院新疆理化技术研究所 | Method for inductively producing snow lotus somatic embryo |
| CN102487720B (en) * | 2011-12-30 | 2013-09-18 | 中国科学院新疆理化技术研究所 | Large-scale artificial planting method for Xinjiang saussurea involucrata |
-
2013
- 2013-04-02 CN CN201310112965.5A patent/CN103141293B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN103141293A (en) | 2013-06-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105052655B (en) | Oil tea implantation methods | |
| CN102550252B (en) | Cultivation method of Dendrobium officinale | |
| CN102577803B (en) | Method for rapidly culturing improved mulberry seedlings by using sheared waste mulberry branches | |
| CN104322257A (en) | Method for normatively planting dendrobium officinale in simple greenhouse | |
| CN102860258B (en) | Clonal tissue culture breeding method for camphor tree | |
| CN106134896A (en) | A kind of implantation methods of lemon | |
| CN103828717A (en) | Rapid propagating and seedling culturing method by virtue of Bletilla tubers | |
| CN102487676A (en) | Method of planting herba houttuyniae in greenhouse | |
| CN102487720B (en) | Large-scale artificial planting method for Xinjiang saussurea involucrata | |
| CN104770279A (en) | Senecio cineraria plug seedling culture transplanting method | |
| CN106561252A (en) | High-yield planting method of watermelons | |
| CN103460954A (en) | Early-stage culture method for white gourd | |
| CN103694039B (en) | The light cultivation matrix of Hubei Province, a kind of river Camellia fraternal breeding and container seedling culture method thereof | |
| CN103493654A (en) | High-yield corn cultivating method | |
| CN106508383A (en) | Bird king tea cutting reproduction method suitable for karst region | |
| CN104620831A (en) | Lotus root protected medium cultivation method | |
| CN110178690B (en) | Application of carbon nano sol in strawberry cultivation, strawberry seedling cultivation medium, matrix and strawberry seedling cultivation method | |
| CN104770177B (en) | A kind of Rosa roxburghii isolated shoot method for culturing seedlings and the application in Rocky Desertification Control | |
| CN105052465A (en) | Mint planting method | |
| CN107241996A (en) | A kind of cultural method of the peaceful root of purple-flowered peucedanum | |
| CN103141293B (en) | Scale planting method for increasing survival rate of saussurea obvallata | |
| CN103964963A (en) | Wormcast rice seedling raising matrix and seedling raising method | |
| CN104620801A (en) | Technical method for cowpea planting | |
| CN103444520A (en) | Breeding technology of novel strawberry virus-free seedling | |
| CN105493819A (en) | Factory-like rapid cultivation method for dicrano pteris pedata seedlings |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |